Carg17367-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg17367-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	CASP-like protein
Location	Carg_Chr06: 9091274 .. 9092963 (+)
Sequence length	842
RNA-Seq Expression	Carg17367-RA
Synteny	Carg17367-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTAATCGTCTCAAAGAGGGAACATGGAAGTGGAGAGAAGATCAGTGGAAAAGGAAGAAGAAGAAGACATGAACAAGAACGTAATGCTTAACCATATGGCTCAAATGGGTTTTAGGGTTTTGGGCTTTGTATTGAGCTTAGTGGCAGCCATTGTCGTGGGACTCAACAAACAGTCCAAGGTTGTTCCTCTTACTGTCTCCCTCAACCTCCCTCCTTTGGATTATACTCTCACAGCTAACTGGCATTACTTGTCTGCTCTCGTGTAAGTCTTTTCTCTCTCCCGCTCCGTTTTTTCTTTCTATACCTTCATTTTCGTCTTTCGAGTTTAAATCGAGCCCGGTACGAAGATTTGAATGTCTGCCCTTTTAAAACATGTTTGTTTTAATGTTTTTTTTTCGGTATTTTCACTCAAATAGTGATTTCTTATGTTTTTAGTTTAGCTGGAGATTTAAACTCGTGACGGTTTAGTTGAAGATGATCAGTATGTTGAGGATTATTGGGAGTGAGTCTCACGTTGGTTAATTTAGTGGAAGATCATGGGTTTATAAGTGAGAAATACTATCACCATTGATACGAGGCCTTTTGGGAAAGCCCAAAGCAAAGTCACGAGAGCTTATTATACTGAACAATATCATATCATCATAGAGAGTCGTGATTCCTAACATAATATCAGAGTCATGCCCTAAACTTAGCCATGTCAAAAGTGACTAAAGTGTCGAACAAAGGGTCTACTTTGTTTGAGGGCGCTTGAGAAAGGAGTCGAGCCTCAATTAAGGGAGAGACTATCTAAATTTAAACGTGATTTGTGGACATCAATAAAATAAAGAAGGTGATAATATTTCGGGTTTTCAAAAAATTTAATTTTTAGGTAGAATTAGAAATTAACAATAATTGACAATAATGAATATGGTCGGATATTTGTTAAAAATATAAATACGATCAACGGGTTAGTTTTTGTACTTTTACGAGCAAAATAATTAAACTTTTAGTGTTAATTATTGAGTTAAGTATAGCTATTATGAATTTGGTACCTTGACACGTTCGATATTCATATACATATAGATAGAAGTGAAGGTGGTATTGGATTAAAAGTATCTCGTGTTTTTTTAGGTATCTTTTAGCGACGAATGTGATAGCATGTTCGTACAGCTTCCTATCGTTCGTTTTTTTGTTAAAAAACAAGAACAAGGACAACATCTTAGCGTTATTGGTAATTGTTCTCGACACGGTGATGGTGGCGCTGCTCTTCTCCAGCAGCGGAGCTGCAGCGGCGGTCGGAGTCATTGCGTACCACGGGAACTCACACGTGCAATGGAATAAAGTGTGCGATGTATATGGTAGATTTTGCAAGCAAATGGCAGCGTCTACTGTGCTCTCTCTTGCCGGTGCTGTAGTATTTATGTTGCTAGTTGTGTTGGCTTCTGTGGGCCTCCAGAAGAGGCCCAAATAAATGGGCTGGGCTTTGGGCCTGTATTTAAAGCTTCTTCACGGCCCATCTAAATGGGCTTTGGCTCTATTCCTCACAGCCCAATTTAAAGGTTAGGTTATCAGTTGTGGAATTGAAAATGTTACAAGAAGACTATTTATGCATTATATTTTTATTTACCTCAATCTCTTTTTCTCTAAAAAAAATAAAAATAAATTATTTATCAACCGAGCGTAGTTCAATCATTAAAATATTTAATATTTT

mRNA sequence

CCTAATCGTCTCAAAGAGGGAACATGGAAGTGGAGAGAAGATCAGTGGAAAAGGAAGAAGAAGAAGACATGAACAAGAACGTAATGCTTAACCATATGGCTCAAATGGGTTTTAGGGTTTTGGGCTTTGTATTGAGCTTAGTGGCAGCCATTGTCGTGGGACTCAACAAACAGTCCAAGGTTGTTCCTCTTACTGTCTCCCTCAACCTCCCTCCTTTGGATTATACTCTCACAGCTAACTGGCATTACTTGTCTGCTCTCGTGTATCTTTTAGCGACGAATGTGATAGCATGTTCGTACAGCTTCCTATCGTTCGTTTTTTTGTTAAAAAACAAGAACAAGGACAACATCTTAGCGTTATTGGTAATTGTTCTCGACACGGTGATGGTGGCGCTGCTCTTCTCCAGCAGCGGAGCTGCAGCGGCGGTCGGAGTCATTGCGTACCACGGGAACTCACACGTGCAATGGAATAAAGTGTGCGATGTATATGGTAGATTTTGCAAGCAAATGGCAGCGTCTACTGTGCTCTCTCTTGCCGGTGCTGTAGTATTTATGTTGCTAGTTGTGTTGGCTTCTGTGGGCCTCCAGAAGAGGCCCAAATAAATGGGCTGGGCTTTGGGCCTGTATTTAAAGCTTCTTCACGGCCCATCTAAATGGGCTTTGGCTCTATTCCTCACAGCCCAATTTAAAGGTTAGGTTATCAGTTGTGGAATTGAAAATGTTACAAGAAGACTATTTATGCATTATATTTTTATTTACCTCAATCTCTTTTTCTCTAAAAAAAATAAAAATAAATTATTTATCAACCGAGCGTAGTTCAATCATTAAAATATTTAATATTTT

Coding sequence (CDS)

ATGGAAGTGGAGAGAAGATCAGTGGAAAAGGAAGAAGAAGAAGACATGAACAAGAACGTAATGCTTAACCATATGGCTCAAATGGGTTTTAGGGTTTTGGGCTTTGTATTGAGCTTAGTGGCAGCCATTGTCGTGGGACTCAACAAACAGTCCAAGGTTGTTCCTCTTACTGTCTCCCTCAACCTCCCTCCTTTGGATTATACTCTCACAGCTAACTGGCATTACTTGTCTGCTCTCGTGTATCTTTTAGCGACGAATGTGATAGCATGTTCGTACAGCTTCCTATCGTTCGTTTTTTTGTTAAAAAACAAGAACAAGGACAACATCTTAGCGTTATTGGTAATTGTTCTCGACACGGTGATGGTGGCGCTGCTCTTCTCCAGCAGCGGAGCTGCAGCGGCGGTCGGAGTCATTGCGTACCACGGGAACTCACACGTGCAATGGAATAAAGTGTGCGATGTATATGGTAGATTTTGCAAGCAAATGGCAGCGTCTACTGTGCTCTCTCTTGCCGGTGCTGTAGTATTTATGTTGCTAGTTGTGTTGGCTTCTGTGGGCCTCCAGAAGAGGCCCAAATAA

Protein sequence

MEVERRSVEKEEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTVSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIVLDTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFMLLVVLASVGLQKRPK

Homology

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BLAST of Carg17367-RA vs. NCBI nr
Match: KAG7028955.1 (hypothetical protein SDJN02_10138 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 354.0 bits (907), Expect = 8.2e-94
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MEVERRSVEKEEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTVSL 60
           MEVERRSVEKEEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTVSL
Sbjct: 1   MEVERRSVEKEEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTVSL 60

Query: 61  NLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIVLDTV 120
           NLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIVLDTV
Sbjct: 61  NLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIVLDTV 120

Query: 121 MVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFMLLV 180
           MVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFMLLV
Sbjct: 121 MVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFMLLV 180

Query: 181 VLASVGLQKRPK 193
           VLASVGLQKRPK
Sbjct: 181 VLASVGLQKRPK 192

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BLAST of Carg17367-RA vs. NCBI nr
Match: KAG6597496.1 (hypothetical protein SDJN03_10676, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 350.9 bits (899), Expect = 7.0e-93
Identity = 191/192 (99.48%), Postives = 191/192 (99.48%), Query Frame = 0


Query: 1   MEVERRSVEKEEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTVSL 60
           MEVERRSVEKEEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTVSL
Sbjct: 1   MEVERRSVEKEEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTVSL 60

Query: 61  NLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIVLDTV 120
           NLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNK NILALLVIVLDTV
Sbjct: 61  NLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKANILALLVIVLDTV 120

Query: 121 MVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFMLLV 180
           MVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFMLLV
Sbjct: 121 MVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFMLLV 180

Query: 181 VLASVGLQKRPK 193
           VLASVGLQKRPK
Sbjct: 181 VLASVGLQKRPK 192

[Close]

BLAST of Carg17367-RA vs. NCBI nr
Match: XP_022943606.1 (CASP-like protein 1E2 [Cucurbita moschata])

HSP 1 Score: 343.2 bits (879), Expect = 1.5e-90
Identity = 189/195 (96.92%), Postives = 191/195 (97.95%), Query Frame = 0


Query: 1   MEVERRSV---EKEEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLT 60
           MEVERRSV   E+EEEEDMNKNVMLNHMAQMGFR LGFVLSLVAAIVVGLNKQSKVVPLT
Sbjct: 1   MEVERRSVKKEEEEEEEDMNKNVMLNHMAQMGFRFLGFVLSLVAAIVVGLNKQSKVVPLT 60

Query: 61  VSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIVL 120
           VSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKD+ILALLVIVL
Sbjct: 61  VSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDSILALLVIVL 120

Query: 121 DTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFM 180
           DTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFM
Sbjct: 121 DTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFM 180

Query: 181 LLVVLASVGLQKRPK 193
           LLVVLASVGLQKRPK
Sbjct: 181 LLVVLASVGLQKRPK 195

[Close]

BLAST of Carg17367-RA vs. NCBI nr
Match: XP_023540234.1 (CASP-like protein 1E2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 342.4 bits (877), Expect = 2.5e-90
Identity = 188/195 (96.41%), Postives = 191/195 (97.95%), Query Frame = 0


Query: 1   MEVERRSVEKEEEE---DMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLT 60
           MEV RRSV+KEEEE   DMNKNVM+NHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLT
Sbjct: 1   MEVVRRSVKKEEEEEQQDMNKNVMVNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLT 60

Query: 61  VSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIVL 120
           VSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVF+LKNKNKDNILALLVIVL
Sbjct: 61  VSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFMLKNKNKDNILALLVIVL 120

Query: 121 DTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFM 180
           DTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFM
Sbjct: 121 DTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFM 180

Query: 181 LLVVLASVGLQKRPK 193
           LLVVLASVGLQKRPK
Sbjct: 181 LLVVLASVGLQKRPK 195

[Close]

BLAST of Carg17367-RA vs. NCBI nr
Match: XP_022973722.1 (CASP-like protein 1E2 [Cucurbita maxima])

HSP 1 Score: 340.1 bits (871), Expect = 1.2e-89
Identity = 186/194 (95.88%), Postives = 190/194 (97.94%), Query Frame = 0


Query: 1   MEVERRSVEK--EEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTV 60
           MEVERRSV+K  EEEEDMNKNVM+NHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTV
Sbjct: 1   MEVERRSVKKGEEEEEDMNKNVMVNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTV 60

Query: 61  SLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIVLD 120
           SLNLPPLDYTL ANWHYLSALVYLL+TNVIACSYSFLSF FLLKNKNKDNI+ALLVIVLD
Sbjct: 61  SLNLPPLDYTLIANWHYLSALVYLLSTNVIACSYSFLSFAFLLKNKNKDNIVALLVIVLD 120

Query: 121 TVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFML 180
           TVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFML
Sbjct: 121 TVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFML 180

Query: 181 LVVLASVGLQKRPK 193
           LVVLASVGLQKRPK
Sbjct: 181 LVVLASVGLQKRPK 194

[Close]

BLAST of Carg17367-RA vs. ExPASy Swiss-Prot
Match: C6SZP8 (CASP-like protein 1E2 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.4e-43
Identity = 97/183 (53.01%), Postives = 131/183 (71.58%), Query Frame = 0


Query: 8   VEKEEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTVSLNLPPLDY 67
           VE +E E M    +   ++ +  R+L F ++LVAAIV+ ++KQ+KVVP+ +S +LPPLD 
Sbjct: 4   VESKEREVMVAKPVAVGVSDLLLRLLAFTVTLVAAIVIAVDKQTKVVPIQLSDSLPPLDV 63

Query: 68  TLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIVLDTVMVALLFS 127
            LTA WH +SA+VY L TN IAC+Y+ LS +  L N+ K   L  L+ VLD  MVALLFS
Sbjct: 64  PLTAKWHQMSAIVYFLVTNAIACTYAVLSLLLALVNRGKSKGLWTLIAVLDAFMVALLFS 123

Query: 128 SSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFMLLVVLASVGL 187
            +GAAAAVGV+ Y GNSHV WNKVC+V+G+FC QMAAS  +SL G++ F+LLV++  V L
Sbjct: 124 GNGAAAAVGVLGYKGNSHVNWNKVCNVFGKFCDQMAASIGVSLIGSLAFLLLVIIPGVRL 183

Query: 188 QKR 191
            +R
Sbjct: 184 HRR 186

[Close]

BLAST of Carg17367-RA vs. ExPASy Swiss-Prot
Match: C6TBD0 (CASP-like protein 1E1 OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 9.9e-42
Identity = 94/181 (51.93%), Postives = 129/181 (71.27%), Query Frame = 0


Query: 10  KEEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTVSLNLPPLDYTL 69
           KE E  + K V +  +  +  R+L F ++LVAAIV+ ++KQ+K+VP+ +S + PPL+  L
Sbjct: 7   KEREVMVAKPVAVVGVCDLLLRLLAFTVTLVAAIVIAVDKQTKLVPIQLSDSFPPLNVPL 66

Query: 70  TANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIVLDTVMVALLFSSS 129
           TA WH +SA VY L TN IAC+Y+ +S +  L N+ K   L  L+ VLDT MVALLFS +
Sbjct: 67  TAKWHQMSAFVYFLVTNAIACTYAAMSLLLALVNRGKSKGLWTLIAVLDTFMVALLFSGN 126

Query: 130 GAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFMLLVVLASVGLQK 189
           GAAAAVG++ Y GNSHV WNKVC+V+G+FC QMAAS  +SL G++ F+LLVV+  V L +
Sbjct: 127 GAAAAVGILGYKGNSHVNWNKVCNVFGKFCDQMAASIGVSLIGSLAFLLLVVIPVVRLHR 186

Query: 190 R 191
           R
Sbjct: 187 R 187

[Close]

BLAST of Carg17367-RA vs. ExPASy Swiss-Prot
Match: B9RT04 (CASP-like protein 1E1 OS=Ricinus communis OX=3988 GN=RCOM_0680180 PE=2 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 4.3e-37
Identity = 82/162 (50.62%), Postives = 117/162 (72.22%), Query Frame = 0


Query: 31  RVLGFVLSLVAAIVVGLNKQSKVVPLTVSLNLPPLDYTLTANWHYLSALVYLLATNVIAC 90
           RVL FVL+  AAIV G+N Q++ VP+ ++ ++PPL   + A WHYLSA V+ + +N IAC
Sbjct: 34  RVLAFVLTSTAAIVHGVNNQTETVPIQLTSSMPPLYVPVVAKWHYLSAFVFFVVSNAIAC 93

Query: 91  SYSFLSFVFLLKNKNKDNILALLVIVLDTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNK 150
           SY+ +S +     K     +  +++ LD +MVALLFSS+GAA A+GV+ Y GNSHV+WNK
Sbjct: 94  SYAAISVMLSFCGKKS---MVPIILTLDLLMVALLFSSNGAATAIGVMGYKGNSHVKWNK 153

Query: 151 VCDVYGRFCKQMAASTVLSLAGAVVFMLLVVLASVGLQKRPK 193
           VC+V+G+FC Q+AAS VLSL G++VF+LLV+L +  L  + K
Sbjct: 154 VCNVFGKFCNQVAASVVLSLIGSIVFVLLVMLTAFRLHNKSK 192

[Close]

BLAST of Carg17367-RA vs. ExPASy Swiss-Prot
Match: B9HMP6 (CASP-like protein 1E1 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_820934 PE=3 SV=2)

HSP 1 Score: 155.6 bits (392), Expect = 5.6e-37
Identity = 91/190 (47.89%), Postives = 129/190 (67.89%), Query Frame = 0


Query: 3   VERRSVEKEEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTVSLNL 62
           +ERR V  + E     +++L        RVL  VL+L AAIV+G++KQ+KVVP+ +   L
Sbjct: 15  LERRVVASQSEGASTCDLLL--------RVLALVLTLAAAIVLGVDKQTKVVPIKIVDTL 74

Query: 63  PPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIVLDTVMV 122
           P ++  ++A WHYLSA  Y +A+N IACSY+ LS V  +  K     +  +VIVLD +MV
Sbjct: 75  PAINLPVSAKWHYLSAFTYSVASNAIACSYAALSLVLAVSGKKG---IMSIVIVLDLLMV 134

Query: 123 ALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFMLLVVL 182
           A+LFSS+GAA A+G++ Y GNSHV+W KVC V+GRFC Q+A S  LSL G+++F+LLV +
Sbjct: 135 AMLFSSNGAALAIGLMGYQGNSHVRWTKVCHVFGRFCNQVAVSISLSLLGSILFLLLVGI 193

Query: 183 ASVGLQKRPK 193
            S+ L K+ K
Sbjct: 195 TSLRLHKKSK 193

[Close]

BLAST of Carg17367-RA vs. ExPASy Swiss-Prot
Match: A7NW78 (CASP-like protein 1E2 OS=Vitis vinifera OX=29760 GN=VIT_05s0020g01820 PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 2.8e-36
Identity = 81/160 (50.62%), Postives = 113/160 (70.62%), Query Frame = 0


Query: 31  RVLGFVLSLVAAIVVGLNKQSKVVPLTVSLNLPPLDYTLTANWHYLSALVYLLATNVIAC 90
           RV+   L+LVAA+++G++KQ+KVV L +   LPP+D  +TA W YLSA VY + +N IAC
Sbjct: 35  RVVALALTLVAAVLLGVDKQTKVVSLQLLPTLPPMDVPVTAKWRYLSAFVYFVVSNAIAC 94

Query: 91  SYSFLSFVFLLKNKNKDNILALLVIVLDTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNK 150
           SY+ LS +  + N   +  L L + V+D VMVALLFSS+GAA A+G++ Y GNS V+W K
Sbjct: 95  SYAALSLLLSVGNSKGNKGLGLAITVMDLVMVALLFSSNGAAGAIGLMGYEGNSRVRWGK 154

Query: 151 VCDVYGRFCKQMAASTVLSLAGAVVFMLLVVLASVGLQKR 191
           VC+V+G+FC Q+A +  LS  G + F LLVV+A+  L KR
Sbjct: 155 VCNVFGKFCNQVAVALGLSFFGGLAFFLLVVMAAFALNKR 194

[Close]

BLAST of Carg17367-RA vs. ExPASy TrEMBL
Match: A0A6J1FS54 (CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111448322 PE=3 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 7.0e-91
Identity = 189/195 (96.92%), Postives = 191/195 (97.95%), Query Frame = 0


Query: 1   MEVERRSV---EKEEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLT 60
           MEVERRSV   E+EEEEDMNKNVMLNHMAQMGFR LGFVLSLVAAIVVGLNKQSKVVPLT
Sbjct: 1   MEVERRSVKKEEEEEEEDMNKNVMLNHMAQMGFRFLGFVLSLVAAIVVGLNKQSKVVPLT 60

Query: 61  VSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIVL 120
           VSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKD+ILALLVIVL
Sbjct: 61  VSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDSILALLVIVL 120

Query: 121 DTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFM 180
           DTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFM
Sbjct: 121 DTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFM 180

Query: 181 LLVVLASVGLQKRPK 193
           LLVVLASVGLQKRPK
Sbjct: 181 LLVVLASVGLQKRPK 195

[Close]

BLAST of Carg17367-RA vs. ExPASy TrEMBL
Match: A0A6J1I8B2 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111472298 PE=3 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 5.9e-90
Identity = 186/194 (95.88%), Postives = 190/194 (97.94%), Query Frame = 0


Query: 1   MEVERRSVEK--EEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTV 60
           MEVERRSV+K  EEEEDMNKNVM+NHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTV
Sbjct: 1   MEVERRSVKKGEEEEEDMNKNVMVNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTV 60

Query: 61  SLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIVLD 120
           SLNLPPLDYTL ANWHYLSALVYLL+TNVIACSYSFLSF FLLKNKNKDNI+ALLVIVLD
Sbjct: 61  SLNLPPLDYTLIANWHYLSALVYLLSTNVIACSYSFLSFAFLLKNKNKDNIVALLVIVLD 120

Query: 121 TVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFML 180
           TVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFML
Sbjct: 121 TVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFML 180

Query: 181 LVVLASVGLQKRPK 193
           LVVLASVGLQKRPK
Sbjct: 181 LVVLASVGLQKRPK 194

[Close]

BLAST of Carg17367-RA vs. ExPASy TrEMBL
Match: A0A6J1IJ94 (CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111474829 PE=3 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 8.6e-89
Identity = 187/196 (95.41%), Postives = 190/196 (96.94%), Query Frame = 0


Query: 1   MEVERRSVEK--EEEEDM--NKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPL 60
           MEVERRSV+K  EEEEDM  NKNVM+NHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPL
Sbjct: 1   MEVERRSVKKGEEEEEDMNKNKNVMVNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPL 60

Query: 61  TVSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIV 120
           TVSLNLPPLDYTL ANWHYLSALVYLL+TNVIACSYSFLSF FLLKNKNKDNILALLVIV
Sbjct: 61  TVSLNLPPLDYTLIANWHYLSALVYLLSTNVIACSYSFLSFAFLLKNKNKDNILALLVIV 120

Query: 121 LDTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVF 180
           LDTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVF
Sbjct: 121 LDTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVF 180

Query: 181 MLLVVLASVGLQKRPK 193
           MLLVVLASVGLQKRPK
Sbjct: 181 MLLVVLASVGLQKRPK 196

[Close]

BLAST of Carg17367-RA vs. ExPASy TrEMBL
Match: A0A0A0LAI6 (CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G151490 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.3e-68
Identity = 155/197 (78.68%), Postives = 165/197 (83.76%), Query Frame = 0


Query: 1   MEVERRSVEKEEE---EDMNK--NVMLN-HMAQMGFRVLGFVLSLVAAIVVGLNKQSKVV 60
           MEVERR  + EEE   E+M K  N   N H  +M FRVLGFVLS VAAIVVGLN Q+KVV
Sbjct: 1   MEVERRRGKMEEERKNEEMKKMGNDYDNYHKVEMVFRVLGFVLSFVAAIVVGLNNQTKVV 60

Query: 61  PLTVSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLV 120
           PL VSLN PPLDYT  A WHYLSA VYLLATN+IACSYSFLS   LLKNK+KDNIL LL+
Sbjct: 61  PLAVSLNSPPLDYTFIAKWHYLSAFVYLLATNIIACSYSFLSLFLLLKNKSKDNILGLLI 120

Query: 121 IVLDTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAV 180
           IVLDTVMVALLFS SGAA AVGVIAY GN+HVQWNKVCD+YGRFCKQ+AASTVLSLAGAV
Sbjct: 121 IVLDTVMVALLFSGSGAAGAVGVIAYQGNTHVQWNKVCDIYGRFCKQVAASTVLSLAGAV 180

Query: 181 VFMLLVVLASVGLQKRP 192
           VFM LVVLASVGLQKRP
Sbjct: 181 VFMSLVVLASVGLQKRP 197

[Close]

BLAST of Carg17367-RA vs. ExPASy TrEMBL
Match: A0A1S3AWX9 (CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103483799 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.2e-68
Identity = 153/200 (76.50%), Postives = 164/200 (82.00%), Query Frame = 0


Query: 1   MEVERRSVEKEEE---EDMNKNVMLN------HMAQMGFRVLGFVLSLVAAIVVGLNKQS 60
           +E ERR  + EEE   E+M K  M N      H  +M FRVLGFVLS VAAIVVGLN Q+
Sbjct: 3   VETERRRGKMEEERKNEEMKKIKMGNYYDNYYHKVEMVFRVLGFVLSFVAAIVVGLNNQT 62

Query: 61  KVVPLTVSLNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILA 120
           KVVPLTVSLN PPLDYT  A WHYLSA VYLLATN+IACSYSF+S   LLK K+KDNIL 
Sbjct: 63  KVVPLTVSLNTPPLDYTFIAKWHYLSAFVYLLATNIIACSYSFVSLFLLLKKKSKDNILG 122

Query: 121 LLVIVLDTVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLA 180
           LL+IVLDTVMVALLFS SGAA AVGVIAY GNSHVQWNKVCD+YGRFCKQ+AASTVLSLA
Sbjct: 123 LLIIVLDTVMVALLFSGSGAAGAVGVIAYQGNSHVQWNKVCDIYGRFCKQVAASTVLSLA 182

Query: 181 GAVVFMLLVVLASVGLQKRP 192
           GAVVFM LVVLASVGLQKRP
Sbjct: 183 GAVVFMSLVVLASVGLQKRP 202

[Close]

BLAST of Carg17367-RA vs. TAIR 10
Match: AT4G15630.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 139.8 bits (351), Expect = 2.3e-33
Identity = 84/187 (44.92%), Postives = 125/187 (66.84%), Query Frame = 0


Query: 2   EVERRSVEKEEEEDMNKNVMLNHMAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLTVSLN 61
           +V+   +EK ++E  ++  +     ++  RVL  VL++VAA V+G+ KQ+KVVP+ +   
Sbjct: 8   KVDGMEMEKGKKESGSRKGL-----ELTMRVLALVLTMVAATVLGVAKQTKVVPIKLIPT 67

Query: 62  LPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNK-DNILALLVIVLDTV 121
           LPPL+ + TA   YLSA VY ++ N IAC Y+ +S V ++ +K K    L + V++ D +
Sbjct: 68  LPPLNVSTTAKASYLSAFVYNISANAIACGYTAISIVIVMISKGKRSKSLLMAVLIGDLM 127

Query: 122 MVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFMLLV 181
           MVALLFSS+GAA A+G++  HGN HV W KVC V+G+FC Q A S  ++L  +VVFMLLV
Sbjct: 128 MVALLFSSTGAAGAIGLMGRHGNKHVMWKKVCGVFGKFCNQAAVSVAITLIASVVFMLLV 187

Query: 182 VLASVGL 188
           VL ++ L
Sbjct: 188 VLDALKL 189

[Close]

BLAST of Carg17367-RA vs. TAIR 10
Match: AT4G15620.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 136.0 bits (341), Expect = 3.3e-32
Identity = 84/189 (44.44%), Postives = 120/189 (63.49%), Query Frame = 0


Query: 8   VEKEEEEDMNKNVMLNHMAQMG--------FRVLGFVLSLVAAIVVGLNKQSKVVPLTVS 67
           +E E + +MN   M     ++G         RVL  +L++ AA V+G+ KQ+KVV + + 
Sbjct: 1   MEHEGKNNMNGMEMEKGKRELGSRKGVELTMRVLALILTMAAATVLGVAKQTKVVSIKLI 60

Query: 68  LNLPPLDYTLTANWHYLSALVYLLATNVIACSYSFLSFVFLLKNKNKDNILALLVIVL-D 127
             LPPLD T TA   YLSA VY ++ N IAC Y+ +S   L+ ++ + +   L+V++L D
Sbjct: 61  PTLPPLDITTTAKASYLSAFVYNISVNAIACGYTAISIAILMISRGRRSKKLLMVVLLGD 120

Query: 128 TVMVALLFSSSGAAAAVGVIAYHGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFML 187
            VMVALLFS +GAA+A+G++  HGN HV W KVC V+G+FC + A S  L+L  AVVFM 
Sbjct: 121 LVMVALLFSGTGAASAIGLMGLHGNKHVMWKKVCGVFGKFCHRAAPSLPLTLLAAVVFMF 180

[Close]

BLAST of Carg17367-RA vs. TAIR 10
Match: AT4G20390.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 77.4 bits (189), Expect = 1.4e-14
Identity = 49/160 (30.63%), Postives = 90/160 (56.25%), Query Frame = 0


Query: 28  MGFRVLGFVLSLVAAIVVGLNKQSKVVPLTVSLNLPPLDYTLTANWHYLSALVYLLATNV 87
           +G R+  F+ +L AAIV+ LNK++K + +  ++   P+  TLTA + +  A V+ +  NV
Sbjct: 20  LGLRIFAFMATLAAAIVMSLNKETKTL-VVATIGTVPIKATLTAKFQHTPAFVFFVIANV 79

Query: 88  IACSYSFLSFVFLLKNKNKD--NILALLVIVLDTVMVALLFSSSGAAAAVGVIAYHGNSH 147
           +   ++ L  V  + ++  +   +  L + +LD +   L+ +++ AA  V  +  +GN H
Sbjct: 80  MVSFHNLLMIVVQIFSRKLEYKGLRLLSIAILDMLNATLVSAAANAAVFVAELGKNGNKH 139

Query: 148 VQWNKVCDVYGRFCKQMAASTVLSLAGAVVFMLLVVLASV 186
            +WNKVCD +  +C   A + + + AG V+ MLLV   S+
Sbjct: 140 AKWNKVCDRFTTYCDHGAGAIIAAFAG-VILMLLVSAVSI 177

[Close]

BLAST of Carg17367-RA vs. TAIR 10
Match: AT4G15610.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 74.3 bits (181), Expect = 1.2e-13
Identity = 49/170 (28.82%), Postives = 92/170 (54.12%), Query Frame = 0


Query: 25  MAQMGFRVLGFVLSLVAAIVVGLNKQSKVVPLT-VSLNLPPLDYTLTANWHYLSALVYLL 84
           M Q+  R + F  +L + +V+  +KQ+K + L    + +P  ++T +       AL+Y +
Sbjct: 28  MTQVVLRFVLFAATLTSIVVMVTSKQTKNIFLPGTPIRIPAAEFTNS------PALIYFV 87

Query: 85  ATNVIACSYSFLS---FVFLLKNKNKDNILALLVIVLDTVMVALLFSSSGAAAAVGVIAY 144
               +AC YS +S    V   K  +   +L L + ++D VMV ++ S++GA   V  +  
Sbjct: 88  VALSVACFYSIVSTFVTVSAFKKHSCSAVLLLNLAIMDAVMVGIVASATGAGGGVAYLGL 147

Query: 145 HGNSHVQWNKVCDVYGRFCKQMAASTVLSLAGAVVFMLLVVLASVGLQKR 191
            GN  V+W K+C +Y +FC+ +  +  +SL  +VV +LL +++ + L K+
Sbjct: 148 KGNKEVRWGKICHIYDKFCRHVGGAIAVSLFASVVLLLLSIISVLSLYKK 191

[Close]

BLAST of Carg17367-RA vs. TAIR 10
Match: AT5G44550.1 (Uncharacterised protein family (UPF0497) )

HSP 1 Score: 73.2 bits (178), Expect = 2.6e-13
Identity = 48/166 (28.92%), Postives = 88/166 (53.01%), Query Frame = 0


Query: 28  MGFRVLGFVLSLVAAIVVGLNKQSKVVPLTVSLNLPPLDYTLTANWHYLSALVYLLATNV 87
           +G R+L F  +L AAIV+GLNK++K   +   +   P+  T TA + +  A V+ +  N 
Sbjct: 20  LGLRLLAFSATLSAAIVMGLNKETKTF-IVGKVGNTPIQATFTAKFDHTPAFVFFVVANA 79

Query: 88  IACSYSFLSFVFLLKNKNKD--NILALLVIVLDTVMVALLFSSSGAAAAVGVIAYHGNSH 147
           +   ++ L     +     +      L V +LD + V L+ +++ AAA +  +  +GN H
Sbjct: 80  MVSFHNLLMIALQIFGGKMEFTGFRLLSVAILDMLNVTLISAAANAAAFMAEVGKNGNKH 139

Query: 148 VQWNKVCDVYGRFCKQMAASTVLSLAGAVVFMLLVVLASVGLQKRP 192
            +W+K+CD +  +C   A + + + AG V+ ML++  AS+    +P
Sbjct: 140 ARWDKICDRFATYCDHGAGALIAAFAG-VILMLIISAASISRLVQP 183

The following BLAST results are available for this feature:

BLAST of Carg17367-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028955.1	8.2e-94	100.00	hypothetical protein SDJN02_10138 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6597496.1	7.0e-93	99.48	hypothetical protein SDJN03_10676, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022943606.1	1.5e-90	96.92	CASP-like protein 1E2 [Cucurbita moschata]	[more]
XP_023540234.1	2.5e-90	96.41	CASP-like protein 1E2 [Cucurbita pepo subsp. pepo]	[more]
XP_022973722.1	1.2e-89	95.88	CASP-like protein 1E2 [Cucurbita maxima]	[more]

BLAST of Carg17367-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
C6SZP8	2.4e-43	53.01	CASP-like protein 1E2 OS=Glycine max OX=3847 PE=2 SV=1	[more]
C6TBD0	9.9e-42	51.93	CASP-like protein 1E1 OS=Glycine max OX=3847 PE=2 SV=1	[more]
B9RT04	4.3e-37	50.62	CASP-like protein 1E1 OS=Ricinus communis OX=3988 GN=RCOM_0680180 PE=2 SV=1	[more]
B9HMP6	5.6e-37	47.89	CASP-like protein 1E1 OS=Populus trichocarpa OX=3694 GN=POPTRDRAFT_820934 PE=3 S...	[more]
A7NW78	2.8e-36	50.63	CASP-like protein 1E2 OS=Vitis vinifera OX=29760 GN=VIT_05s0020g01820 PE=2 SV=1	[more]

BLAST of Carg17367-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FS54	7.0e-91	96.92	CASP-like protein OS=Cucurbita moschata OX=3662 GN=LOC111448322 PE=3 SV=1	[more]
A0A6J1I8B2	5.9e-90	95.88	CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111472298 PE=3 SV=1	[more]
A0A6J1IJ94	8.6e-89	95.41	CASP-like protein OS=Cucurbita maxima OX=3661 GN=LOC111474829 PE=3 SV=1	[more]
A0A0A0LAI6	1.3e-68	78.68	CASP-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G151490 PE=3 SV=1	[more]
A0A1S3AWX9	2.2e-68	76.50	CASP-like protein OS=Cucumis melo OX=3656 GN=LOC103483799 PE=3 SV=1	[more]

BLAST of Carg17367-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15630.1	2.3e-33	44.92	Uncharacterised protein family (UPF0497)	[more]
AT4G15620.1	3.3e-32	44.44	Uncharacterised protein family (UPF0497)	[more]
AT4G20390.1	1.4e-14	30.63	Uncharacterised protein family (UPF0497)	[more]
AT4G15610.1	1.2e-13	28.82	Uncharacterised protein family (UPF0497)	[more]
AT5G44550.1	2.6e-13	28.92	Uncharacterised protein family (UPF0497)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006459	Casparian strip membrane protein	TIGRFAM	TIGR01569	TIGR01569	coord: 29..184 e-value: 3.4E-40 score: 135.3
IPR006702	Casparian strip membrane protein domain	PFAM	PF04535	DUF588	coord: 24..175 e-value: 4.3E-38 score: 130.4
None	No IPR available	PANTHER	PTHR11615	NITRATE, FORMATE, IRON DEHYDROGENASE	coord: 16..190
None	No IPR available	PANTHER	PTHR11615:SF253	CASP-LIKE PROTEIN 1E1	coord: 16..190

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg17367	Carg17367	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg17367-RA:1	Carg17367-RA:1	exon
Carg17367-RA:2	Carg17367-RA:2	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg17367-RA:five_prime_utr	Carg17367-RA:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg17367-RA:cds	Carg17367-RA:cds	CDS
Carg17367-RA:cds	Carg17367-RA:cds_2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg17367-RA:three_prime_utr	Carg17367-RA:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg17367-RA	Carg17367-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding