Carg14391-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg14391-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	GCK domain-containing protein
Location	Carg_Chr04: 9823479 .. 9824015 (+)
Sequence length	537
RNA-Seq Expression	Carg14391-RA
Synteny	Carg14391-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAACTTCGGGGTCGAAACGCCAAGACGCAGAATCAAACGCCGCCGCCGCCGCTAAATCACCGGAAGATCAACAACAATTGGGCGCCCCAAATCACTCTGAGGAAAAATCGCCCGAGGAATCAGGTCAATCGGGAAAGATTTCCGAAAGTGGAGGTGAAGATGAAGGAGGAGAGGATCTAGACGAGGAAGAGGAGGGGGAGTGTGGGTTTTGCTTGTTTATGAAAGGGGGTGGTTGCAGAGATAGCTTCGTAGAATGGGAGAAATGCATTGAGGAGGCGGAGAAGAACAAGGAGGATATTGTGGAGAAGTGCTTTGAAATCACTGGGGCTCTGAAGAAATGTATGGAGGCTCATGCGGATTATTACGAGCCGATTCTGAAGGCGGAGAAAATGGCAGAAAACGAGGCGATTATCGAATTGGAGAAGGAATCAACTCCAAATGCTACCCAGCAACATACGGATTCCATAGACCTGGAGGTGATTGCAGGTACCGAGAATTTGGAAGAACAGAAGGCCTCTGCTGCTGGTAAGTGA

mRNA sequence

ATGTCAACTTCGGGGTCGAAACGCCAAGACGCAGAATCAAACGCCGCCGCCGCCGCTAAATCACCGGAAGATCAACAACAATTGGGCGCCCCAAATCACTCTGAGGAAAAATCGCCCGAGGAATCAGGTCAATCGGGAAAGATTTCCGAAAGTGGAGGTGAAGATGAAGGAGGAGAGGATCTAGACGAGGAAGAGGAGGGGGAGTGTGGGTTTTGCTTGTTTATGAAAGGGGGTGGTTGCAGAGATAGCTTCGTAGAATGGGAGAAATGCATTGAGGAGGCGGAGAAGAACAAGGAGGATATTGTGGAGAAGTGCTTTGAAATCACTGGGGCTCTGAAGAAATGTATGGAGGCTCATGCGGATTATTACGAGCCGATTCTGAAGGCGGAGAAAATGGCAGAAAACGAGGCGATTATCGAATTGGAGAAGGAATCAACTCCAAATGCTACCCAGCAACATACGGATTCCATAGACCTGGAGGTGATTGCAGGTACCGAGAATTTGGAAGAACAGAAGGCCTCTGCTGCTGGTAAGTGA

Coding sequence (CDS)

ATGTCAACTTCGGGGTCGAAACGCCAAGACGCAGAATCAAACGCCGCCGCCGCCGCTAAATCACCGGAAGATCAACAACAATTGGGCGCCCCAAATCACTCTGAGGAAAAATCGCCCGAGGAATCAGGTCAATCGGGAAAGATTTCCGAAAGTGGAGGTGAAGATGAAGGAGGAGAGGATCTAGACGAGGAAGAGGAGGGGGAGTGTGGGTTTTGCTTGTTTATGAAAGGGGGTGGTTGCAGAGATAGCTTCGTAGAATGGGAGAAATGCATTGAGGAGGCGGAGAAGAACAAGGAGGATATTGTGGAGAAGTGCTTTGAAATCACTGGGGCTCTGAAGAAATGTATGGAGGCTCATGCGGATTATTACGAGCCGATTCTGAAGGCGGAGAAAATGGCAGAAAACGAGGCGATTATCGAATTGGAGAAGGAATCAACTCCAAATGCTACCCAGCAACATACGGATTCCATAGACCTGGAGGTGATTGCAGGTACCGAGAATTTGGAAGAACAGAAGGCCTCTGCTGCTGGTAAGTGA

Protein sequence

MSTSGSKRQDAESNAAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEGGEDLDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCMEAHADYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAAGK

Homology

[Close]

BLAST of Carg14391-RA vs. NCBI nr
Match: KAG7032232.1 (hypothetical protein SDJN02_06275, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 337.4 bits (864), Expect = 7.4e-89
Identity = 178/178 (100.00%), Postives = 178/178 (100.00%), Query Frame = 0


Query: 1   MSTSGSKRQDAESNAAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEGGED 60
           MSTSGSKRQDAESNAAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEGGED
Sbjct: 1   MSTSGSKRQDAESNAAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEGGED 60

Query: 61  LDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCMEAHA 120
           LDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCMEAHA
Sbjct: 61  LDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCMEAHA 120

Query: 121 DYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAAGK 179
           DYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAAGK
Sbjct: 121 DYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAAGK 178

[Close]

BLAST of Carg14391-RA vs. NCBI nr
Match: KAG6601449.1 (hypothetical protein SDJN03_06682, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 333.2 bits (853), Expect = 1.4e-87
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 1   MSTSGSKRQDAESNAAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEGGED 60
           MSTSGSKRQDAESNAAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEGGED
Sbjct: 1   MSTSGSKRQDAESNAAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEGGED 60

Query: 61  LDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCMEAHA 120
           LDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCMEAHA
Sbjct: 61  LDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCMEAHA 120

Query: 121 DYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAA 177
           DYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAA
Sbjct: 121 DYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAA 176

[Close]

BLAST of Carg14391-RA vs. NCBI nr
Match: XP_023536143.1 (uncharacterized protein LOC111797387 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 321.6 bits (823), Expect = 4.2e-84
Identity = 170/176 (96.59%), Postives = 172/176 (97.73%), Query Frame = 0


Query: 1   MSTSGSKRQDAESNAAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEGGED 60
           MSTS SK QDAESNAAAA K+PEDQQQLGAPNHSEEKSPEESGQSGKIS SGGEDEGGED
Sbjct: 1   MSTSESKLQDAESNAAAATKTPEDQQQLGAPNHSEEKSPEESGQSGKISGSGGEDEGGED 60

Query: 61  LDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCMEAHA 120
           LDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFE+TGALKKCMEAHA
Sbjct: 61  LDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEVTGALKKCMEAHA 120

Query: 121 DYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAA 177
           DYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAA
Sbjct: 121 DYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAA 176

[Close]

BLAST of Carg14391-RA vs. NCBI nr
Match: XP_022997117.1 (uncharacterized protein LOC111492108 [Cucurbita maxima])

HSP 1 Score: 312.8 bits (800), Expect = 1.9e-81
Identity = 168/177 (94.92%), Postives = 172/177 (97.18%), Query Frame = 0


Query: 1   MSTSGSKRQDAESN-AAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEGGE 60
           MSTS SK QDAESN AAAAAK+PEDQQQLGAPNHSEEKSP+ES QSGKISESGGEDEGGE
Sbjct: 1   MSTSESKLQDAESNAAAAAAKTPEDQQQLGAPNHSEEKSPQESVQSGKISESGGEDEGGE 60

Query: 61  DLDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCMEAH 120
           DLDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFE+TGALKKCMEAH
Sbjct: 61  DLDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEVTGALKKCMEAH 120

Query: 121 ADYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAA 177
           ADYYEPILKAEKMAENEAIIELEKESTPNATQQHTDS DLEVI+GTENLEEQKASAA
Sbjct: 121 ADYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSKDLEVISGTENLEEQKASAA 177

[Close]

BLAST of Carg14391-RA vs. NCBI nr
Match: XP_022957551.1 (transcription elongation factor spt5 [Cucurbita moschata] >XP_022957552.1 transcription elongation factor spt5 [Cucurbita moschata])

HSP 1 Score: 271.9 bits (694), Expect = 3.8e-69
Identity = 146/153 (95.42%), Postives = 147/153 (96.08%), Query Frame = 0


Query: 1   MSTSGSKRQDAESN---AAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEG 60
           MSTS SK QDAESN   AAAAAK+PEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEG
Sbjct: 1   MSTSESKLQDAESNAAAAAAAAKTPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEG 60

Query: 61  GEDLDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCME 120
           GEDLDEEEE ECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCME
Sbjct: 61  GEDLDEEEEEECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCME 120

Query: 121 AHADYYEPILKAEKMAENEAIIELEKESTPNAT 151
           AHADYYEPILKAEKMAENEAIIELEKESTPNAT
Sbjct: 121 AHADYYEPILKAEKMAENEAIIELEKESTPNAT 153

[Close]

BLAST of Carg14391-RA vs. ExPASy TrEMBL
Match: A0A6J1K434 (uncharacterized protein LOC111492108 OS=Cucurbita maxima OX=3661 GN=LOC111492108 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 9.4e-82
Identity = 168/177 (94.92%), Postives = 172/177 (97.18%), Query Frame = 0


Query: 1   MSTSGSKRQDAESN-AAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEGGE 60
           MSTS SK QDAESN AAAAAK+PEDQQQLGAPNHSEEKSP+ES QSGKISESGGEDEGGE
Sbjct: 1   MSTSESKLQDAESNAAAAAAKTPEDQQQLGAPNHSEEKSPQESVQSGKISESGGEDEGGE 60

Query: 61  DLDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCMEAH 120
           DLDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFE+TGALKKCMEAH
Sbjct: 61  DLDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEVTGALKKCMEAH 120

Query: 121 ADYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAA 177
           ADYYEPILKAEKMAENEAIIELEKESTPNATQQHTDS DLEVI+GTENLEEQKASAA
Sbjct: 121 ADYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSKDLEVISGTENLEEQKASAA 177

[Close]

BLAST of Carg14391-RA vs. ExPASy TrEMBL
Match: A0A6J1GZI9 (transcription elongation factor spt5 OS=Cucurbita moschata OX=3662 GN=LOC111458916 PE=4 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 1.8e-69
Identity = 146/153 (95.42%), Postives = 147/153 (96.08%), Query Frame = 0


Query: 1   MSTSGSKRQDAESN---AAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEG 60
           MSTS SK QDAESN   AAAAAK+PEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEG
Sbjct: 1   MSTSESKLQDAESNAAAAAAAAKTPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEG 60

Query: 61  GEDLDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCME 120
           GEDLDEEEE ECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCME
Sbjct: 61  GEDLDEEEEEECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCME 120

Query: 121 AHADYYEPILKAEKMAENEAIIELEKESTPNAT 151
           AHADYYEPILKAEKMAENEAIIELEKESTPNAT
Sbjct: 121 AHADYYEPILKAEKMAENEAIIELEKESTPNAT 153

[Close]

BLAST of Carg14391-RA vs. ExPASy TrEMBL
Match: A0A0A0KU46 (GCK domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G606380 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.7e-60
Identity = 135/178 (75.84%), Postives = 147/178 (82.58%), Query Frame = 0


Query: 1   MSTSGSKRQDAESNAAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEGGED 60
           MSTS SK QDAESN AAA K+ EDQ Q   PN+SEEKS EE+  SGKISESGG DEGGED
Sbjct: 1   MSTSESKLQDAESN-AAATKTLEDQPQSDTPNYSEEKSSEEASLSGKISESGGGDEGGED 60

Query: 61  LDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCMEAHA 120
           ++EEEEGECGFCLFMKGGGCRDSF+ WEKCIEEAEKNKEDIVEKCFE+TGALKKCMEAH 
Sbjct: 61  IEEEEEGECGFCLFMKGGGCRDSFIAWEKCIEEAEKNKEDIVEKCFEVTGALKKCMEAHP 120

Query: 121 DYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAAGK 179
           DYYEPILKAEKMAE EAIIELEKE+TP+A+QQ T+S            +EQKAS  GK
Sbjct: 121 DYYEPILKAEKMAEEEAIIELEKETTPSASQQDTNS------------DEQKASVGGK 165

[Close]

BLAST of Carg14391-RA vs. ExPASy TrEMBL
Match: A0A5A7SVC8 (Transcription elongation factor spt5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1932G00170 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 8.0e-57
Identity = 132/182 (72.53%), Postives = 147/182 (80.77%), Query Frame = 0


Query: 1   MSTSGSKRQDAESNAAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEGGED 60
           MSTS SK QDAESN AAA+K+ EDQ Q  + +HS+EKS EE+  SGKISESGG DEGGED
Sbjct: 19  MSTSESKLQDAESN-AAASKTLEDQPQSDSASHSDEKSSEEASLSGKISESGGGDEGGED 78

Query: 61  L----DEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCM 120
           +    +EEEEGECGFCLFMKGGGCRDSF+ WEKCIEEAEKNKEDIVEKCFE+TGALKKCM
Sbjct: 79  IEEEEEEEEEGECGFCLFMKGGGCRDSFIAWEKCIEEAEKNKEDIVEKCFEVTGALKKCM 138

Query: 121 EAHADYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAA 179
           EAH DYYEPILKAEKMAE EAIIELEKE  P+A+Q+ T+S            +EQKASA 
Sbjct: 139 EAHPDYYEPILKAEKMAEEEAIIELEKEIAPSASQEDTNS------------DEQKASAG 187

[Close]

BLAST of Carg14391-RA vs. ExPASy TrEMBL
Match: A0A6J1G1F8 (uncharacterized protein LOC111449816 OS=Cucurbita moschata OX=3662 GN=LOC111449816 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 8.0e-57
Identity = 135/178 (75.84%), Postives = 144/178 (80.90%), Query Frame = 0


Query: 1   MSTSGSKRQDAESNAAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEGGED 60
           MSTS SK QDAESN AAA K+ ED+QQ G+P   EEKS  ES QSGKI ESGGED     
Sbjct: 1   MSTSNSKLQDAESN-AAATKTLEDEQQSGSPIPPEEKSSGESDQSGKIPESGGEDP---- 60

Query: 61  LDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCMEAHA 120
            +EEEEGECGFCLFMKGGGC+DSFVEWEKCIEEAEKNKEDIVEKCFE+TGALKKCMEAHA
Sbjct: 61  -EEEEEGECGFCLFMKGGGCKDSFVEWEKCIEEAEKNKEDIVEKCFEVTGALKKCMEAHA 120

Query: 121 DYYEPILKAEKMAENEAIIELEKESTPNATQQHTDSIDLEVIAGTENLEEQKASAAGK 179
           DYYEPILKAEKMAE EAIIELEKE TP+A     +S  LEV  G+ N EEQKASA  K
Sbjct: 121 DYYEPILKAEKMAEEEAIIELEKEMTPDA-----NSNVLEVNEGSNNSEEQKASAGSK 167

[Close]

BLAST of Carg14391-RA vs. TAIR 10
Match: AT1G14060.1 (GCK domain-containing protein )

HSP 1 Score: 132.5 bits (332), Expect = 3.4e-31
Identity = 73/131 (55.73%), Postives = 86/131 (65.65%), Query Frame = 0


Query: 22  PEDQQQLGAPNHSEEKSPEESGQSGKI--------SESGGEDEGGEDLDEEEEGECGFCL 81
           P   ++LG  + S     EE G S  +        S+   E+ GG D D EEEGECGFCL
Sbjct: 27  PAKSEKLGDASPSTPAPSEELGDSSTVWRDPEDSESDEKPEEGGGGDADGEEEGECGFCL 86

Query: 82  FMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCMEAHADYYEPILKAEKMA 141
           FMKGGGC+DSFV WE+C+E AEKNKEDIV  C E+T  LKKCM+ H+DYY+PIL AEK A
Sbjct: 87  FMKGGGCKDSFVAWEECVEAAEKNKEDIVTNCMEVTSMLKKCMDEHSDYYQPILAAEKAA 146

Query: 142 ENEAIIELEKE 145
           E E   ELE E
Sbjct: 147 EAEVKKELEAE 157

[Close]

BLAST of Carg14391-RA vs. TAIR 10
Match: AT5G57640.1 (GCK domain-containing protein )

HSP 1 Score: 73.9 bits (180), Expect = 1.4e-13
Identity = 53/131 (40.46%), Postives = 69/131 (52.67%), Query Frame = 0


Query: 23  EDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEG---GEDLD--EEEEGECGFCLFMKG 82
           EDQ+ +       E   +       I + G E+E     EDLD  EEE GEC  C F+KG
Sbjct: 33  EDQRDVEESKTLSEDQVDVKESKTLIEDQGDEEESKTLDEDLDETEEESGECPLCQFIKG 92

Query: 83  GGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGALKKCMEAHADYYEPILKAEKMAENEA 142
           G C+++F E E C +EA+KN ED V KC E     K CM  +  YYEP+L  E  A  + 
Sbjct: 93  GECKEAFTELENCCDEAKKNNEDHVSKCKEARLIFKTCMYDNPVYYEPLLAGEARAMAKM 152

Query: 143 IIEL---EKES 146
           + EL   EKE+
Sbjct: 153 LSELLNAEKEA 163

[Close]

BLAST of Carg14391-RA vs. TAIR 10
Match: AT5G02210.1 (GCK domain-containing protein )

HSP 1 Score: 73.6 bits (179), Expect = 1.9e-13
Identity = 43/90 (47.78%), Postives = 57/90 (63.33%), Query Frame = 0


Query: 59  EDLDEEEEGECGFCLFMKGGGCRDSFVEW-EKC---IEEAEKNKEDIVEKCFEITGALKK 118
           ++++EEE+ +  F  FMKGGGC++SF  W + C    EEAEKNK D+V KC  + G   K
Sbjct: 70  DEINEEEDQK--FVEFMKGGGCKESFTAWNDDCNFTEEEAEKNK-DLVTKCAGLFGTFSK 129

Query: 119 CMEAHADYYEPILKAEKMAENEAIIELEKE 145
           CM+ H+DYY PIL  +K  E      LEKE
Sbjct: 130 CMDTHSDYYHPILAVKKTTEE----HLEKE 152

[Close]

BLAST of Carg14391-RA vs. TAIR 10
Match: AT5G57570.1 (GCK domain-containing protein )

HSP 1 Score: 61.2 bits (147), Expect = 9.5e-10
Identity = 43/122 (35.25%), Postives = 68/122 (55.74%), Query Frame = 0


Query: 13  SNAAAAAKSPEDQQQLGAPNHSEEKSPEESGQSGKISESGGEDEGGEDLDEEEEGECGFC 72
           +++ + A S ED      P+ ++ +   +  +S  + E   E   G+  +EEE GEC  C
Sbjct: 4   ASSVSMAYSDED------PSANQGQFSRDEDESRTLIEDLNETGEGKG-EEEESGECRLC 63

Query: 73  LFMKGGGCRDSFVEWEKCIEEAEKN-KEDIVEKCFEITGALKKCMEAHADYYEPILKAEK 132
            F+KGG C++SF+  E+C++EAE++ +E    KC E+    K CM  +  YYEPIL  E 
Sbjct: 64  RFIKGGECKESFIALEQCVDEAEESGEESDFTKCKEVRAKFKTCMYENPVYYEPILAGEA 118

Query: 133 MA 134
            A
Sbjct: 124 RA 118

[Close]

BLAST of Carg14391-RA vs. TAIR 10
Match: AT1G14048.1 (GCK domain-containing protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.1e-05
Identity = 31/91 (34.07%), Postives = 44/91 (48.35%), Query Frame = 0


Query: 53  GEDEGGEDLDEEEEGECGFCLFMKGGGCRDSFVEWEKCIEEAEKNKEDIVEKCFEITGAL 112
           GE     ++ EEEE    F  FM+GGGC++ F +WE C +EA             +   +
Sbjct: 14  GESVSPNEVREEEEKGEVFSAFMRGGGCKEPFTDWEDCTDEATN---------VGVFAMM 73

Query: 113 KKCME-AHADYYEPILKAEKMAENEAIIELE 143
            KCM     D+Y P L A+K A+     EL+
Sbjct: 74  TKCMVWMLTDHYRPFLAAKKTAQEHIEKELQ 95

The following BLAST results are available for this feature:

BLAST of Carg14391-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7032232.1	7.4e-89	100.00	hypothetical protein SDJN02_06275, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6601449.1	1.4e-87	100.00	hypothetical protein SDJN03_06682, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023536143.1	4.2e-84	96.59	uncharacterized protein LOC111797387 [Cucurbita pepo subsp. pepo]	[more]
XP_022997117.1	1.9e-81	94.92	uncharacterized protein LOC111492108 [Cucurbita maxima]	[more]
XP_022957551.1	3.8e-69	95.42	transcription elongation factor spt5 [Cucurbita moschata] >XP_022957552.1 transc...	[more]

BLAST of Carg14391-RA vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg14391-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K434	9.4e-82	94.92	uncharacterized protein LOC111492108 OS=Cucurbita maxima OX=3661 GN=LOC111492108...	[more]
A0A6J1GZI9	1.8e-69	95.42	transcription elongation factor spt5 OS=Cucurbita moschata OX=3662 GN=LOC1114589...	[more]
A0A0A0KU46	2.7e-60	75.84	GCK domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G606380 PE=4 SV...	[more]
A0A5A7SVC8	8.0e-57	72.53	Transcription elongation factor spt5-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A6J1G1F8	8.0e-57	75.84	uncharacterized protein LOC111449816 OS=Cucurbita moschata OX=3662 GN=LOC1114498...	[more]

BLAST of Carg14391-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14060.1	3.4e-31	55.73	GCK domain-containing protein	[more]
AT5G57640.1	1.4e-13	40.46	GCK domain-containing protein	[more]
AT5G02210.1	1.9e-13	47.78	GCK domain-containing protein	[more]
AT5G57570.1	9.5e-10	35.25	GCK domain-containing protein	[more]
AT1G14048.1	1.1e-05	34.07	GCK domain-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 84..104
None	No IPR available	SMART	SM01227	GCK_2	coord: 67..141 e-value: 4.9E-38 score: 142.3
None	No IPR available	GENE3D	1.10.287.2900		coord: 47..127 e-value: 4.6E-7 score: 31.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..66
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 19..33
None	No IPR available	PANTHER	PTHR34357:SF2	F7A19.14 PROTEIN-RELATED	coord: 18..155
None	No IPR available	PANTHER	PTHR34357	F7A19.14 PROTEIN-RELATED	coord: 18..155
IPR012891	GCK	PFAM	PF07802	GCK	coord: 67..137 e-value: 4.2E-35 score: 120.0

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg14391	Carg14391	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg14391-RA:1	Carg14391-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg14391-RA:cds	Carg14391-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg14391-RA	Carg14391-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006414	translational elongation
molecular_function	GO:0003746	translation elongation factor activity