Carg12869-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12869-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	meiotic recombination protein SPO11-1
Location	Carg_Chr09: 5625825 .. 5628708 (+)
Sequence length	807
RNA-Seq Expression	Carg12869-RA
Synteny	Carg12869-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGGAAATCTCGGAAGCTCGGAGAGAATCGACATCATGAGGAAAATCAGAGGTCACAATTCCATTGAATTTACTCTTTCTCATATTCATTCCATACGGTTTCTGTTTCAACGTTGCTATTTTCTTTTTCGCTTTTCGATTCCGTGTTTTGCAGAGCTAACAAGATCTATGATCGTTGAACTGAGTAATGGAAGCTCCAAGATGATCGCTATCGATCGGTTTAAAAATTACTGCACTAATTCCATTGAAAACTGGTTCGGATTTTGTTTTGCTTGTTTGCAGTTTTTGTTACTTTTGAATCTTCACAGTCGTACCAAATAATGATCACGATCATCATGTTTCTACGTGATTTACTTGACTACGATTATTTAGTTCTCAATACGAATGACAATTTTCGATAATCTTCGATTTGTTATTTACCAATGGATCTGTTGGATCCTATCGGAGGCCGAGTCAATCGGTTGCATAACTTAGCCGCGATTTGTCATTTTCGGAAGTCACTTCTCTGTTTAAACTCATAAGCGTTTAATATGTAGGACCTAGTTTCCAGTCATCCTTTTCGTAATAGACGAATGGTGTTTCAAACAATAGTTTAATCTTTCAACAAAATAAAGTTCTCGAATGTGCAGTGGCCACTATCTTGAAATTGGACATGTGCTTTGGAAATGACATGTTGCCTACCTAAAGAGATGTTTACACGATTATGGCTATTTTTTATGCAGTTGCTGCAGGTTCAATTTATCTGAAGGGAAGGATATTCTAACGCTTCAAAAGGAAAGCCATGTCCGTAGACTAGGTGATTTAGTTTTGTCGACGAATGATGTTATAAAATCGATTCATATAGACTACAGAGTACACTTGTGACTTATTTGATATCATTTTGCTCTATCAGATGTCTTGCTTAGAGTCCTACTCATTATTCAGCAGCTTCTGCGAGAAAATAGACATAGCTCAAAGAGAGATATATACTATATGCATCCCTCTGTCTTTACAGGTAAAGCTAACCACCGTTTAATCCGCAGGCACCGGTAGATTTGTTTAAGATACATGTTTCATAAAATAATTAGCTCTCCAGTTGAGAAGAATTAAATTTCCACATATCCTTCGAATTTATGTTTTCAGCAAATTTGTGGCAGGAATTTTCTGAACATCTCCTTGTTTTGCTTGATATGACAGAGTTTAATGACATTGGCTTCATTGTAATGATGAATATTTTGCAGATCAGTCTGTTGTTGACCGGGCAATCAACGACATCTGCATTCTTTTGCAATGTAGCCGGCACAATCTAAATGTGGTTGGTGCTGCGCTTCATTTTACTTTCTTTCCAATTTATGTTTCCTCCATAGTTATATAATACAGTTCATGTGGGATCTAATCTCAATATGATATTTACAGTCGGCTGCGTGTCACACAAAATGCGGTTCTTTGATAAAATAAACGTATAGTCATTTTTTGTGATCCAACAGCACTAATAGGTGTTGAAATTAGGAAGATACTCTTCAACAGATCAATTGCAGTTTTCTTTAAATCCTATAAACATGTTTGCAAGCTGCTTCTGATGATCATTTCAACTTGTGTCCTTTTGACAGGTTTCTGTTGGTAATGGGTAAGTATCGAGGAGAACATCTGCTCAGTCCAGTAAAGAAAAGACTAGTGCAGAGTGCTGCTTGCGTTCTTGGCATAAGCCTTTACAAGCAGTCTAATTACACAATTATTTGTTTTGTGGGTCGATACCCATATCAGCCTTTTCTATACCATTAGATTTTGTTACATAACAATTTCCTCTTTTTGCTGCAATCTGTTTCATGGAGGAAGAAAATAAAGAACTGAATGTTGACGTAGTCACTTCTTTTACAGGTTGGTGATGGGTTGGTTGAGGTTCGCAGAAGCTGGTAGGAGATTTGATTGCATAAAGAAGCCTAACAATGTAAAAGTTACTACGGTTCTTCATAATGCAATTGCCAATTTTATCACTCTTAGCTGTAACTGATTTGAACATATGCATCTCAATAACATACAGGCCCACTCCATTCCTGTTCAAGTTGAAGAAGTCACAGGTATTGCTGATACTGGATACATAACTTGCCAATCTGTTAGGCTAGAGCTTAAATTAGACACTCGGAATGATACCTTTCGATATGGATGGTCTAGAATTACGTAGTTCTTATTGTAGAGAATTGTTGGGAGGGAGTCCCACGTCCGCTAATTTAGGGAATCATCGTGAGTTTATAAGTAAGGAATACATCTCTATTGGTATGAGGCCAGTGATTCCTAACACTTATCGTATTCCTCCATATTCTTGAAAGCCCTTTTCACCCGATTCGGTTTTCGCTTATGACTATTTTTATCTTTATATGCAGATATTGCTAGTCCTGCGAATTACATACTCGTTGTTGAGAAAGAATCAGGTGAAGGCTATACTGATAAAAGCTGATTTCACGGTTCAAGGAATGAGTTTCACTTTGATCAGTTAAAAGTACCTGGTTCGATTTTTGAACGTGCAGTTTTTCAGCGTCTAGCAAATGACCATTTCTGCAGCAGAAACCGTTGCATTGTAATCTCGGTGAGCATGTCTCAGGTCCTGCAATTGAAAATTCTAATATTCAAATGTTAACATTTTCTTTTTTCAGGGACGTGGATATCCAGATGTTCCTACTAGAAGGTCTAAAGAAATAAATTATGGTCATAAAAACGCTAATCTCATTTTATTATTGATGATGCGAGACATGAAAATATGCACGCATGTATTGCTATCAGGTTTCTGCGGCTTCTCGTTGATGTCTTGGCATTGCCTGCATTTTGTTTGGTAGATTGTGATCCATATGGGTTTGACATTCTGACAACATATCGATTCGGTTCTATGGTTAGGCTCCTCAATTGA

mRNA sequence

ATGGCGGGAAATCTCGGAAGCTCGGAGAGAATCGACATCATGAGGAAAATCAGAGAGCTAACAAGATCTATGATCGTTGAACTGAGTAATGGAAGCTCCAAGATGATCGCTATCGATCGGTTTAAAAATTACTGCACTAATTCCATTGAAAACTGTTGCTGCAGGTTCAATTTATCTGAAGGGAAGGATATTCTAACGCTTCAAAAGGAAAGCCATGTCCGTAGACTAGATGTCTTGCTTAGAGTCCTACTCATTATTCAGCAGCTTCTGCGAGAAAATAGACATAGCTCAAAGAGAGATATATACTATATGCATCCCTCTGTCTTTACAGATCAGTCTGTTGTTGACCGGGCAATCAACGACATCTGCATTCTTTTGCAATGTAGCCGGCACAATCTAAATGTGGTTTCTGTTGGTAATGGGTTGGTGATGGGTTGGTTGAGGTTCGCAGAAGCTGGTAGGAGATTTGATTGCATAAAGAAGCCTAACAATGTAAAAGTTACTACGGCCCACTCCATTCCTGTTCAAGTTGAAGAAGTCACAGATATTGCTAGTCCTGCGAATTACATACTCGTTGTTGAGAAAGAATCAGTTTTTCAGCGTCTAGCAAATGACCATTTCTGCAGCAGAAACCGTTGCATTGTAATCTCGGGACGTGGATATCCAGATGTTCCTACTAGAAGGTTTCTGCGGCTTCTCGTTGATGTCTTGGCATTGCCTGCATTTTGTTTGGTAGATTGTGATCCATATGGGTTTGACATTCTGACAACATATCGATTCGGTTCTATGGTTAGGCTCCTCAATTGA

Coding sequence (CDS)

ATGGCGGGAAATCTCGGAAGCTCGGAGAGAATCGACATCATGAGGAAAATCAGAGAGCTAACAAGATCTATGATCGTTGAACTGAGTAATGGAAGCTCCAAGATGATCGCTATCGATCGGTTTAAAAATTACTGCACTAATTCCATTGAAAACTGTTGCTGCAGGTTCAATTTATCTGAAGGGAAGGATATTCTAACGCTTCAAAAGGAAAGCCATGTCCGTAGACTAGATGTCTTGCTTAGAGTCCTACTCATTATTCAGCAGCTTCTGCGAGAAAATAGACATAGCTCAAAGAGAGATATATACTATATGCATCCCTCTGTCTTTACAGATCAGTCTGTTGTTGACCGGGCAATCAACGACATCTGCATTCTTTTGCAATGTAGCCGGCACAATCTAAATGTGGTTTCTGTTGGTAATGGGTTGGTGATGGGTTGGTTGAGGTTCGCAGAAGCTGGTAGGAGATTTGATTGCATAAAGAAGCCTAACAATGTAAAAGTTACTACGGCCCACTCCATTCCTGTTCAAGTTGAAGAAGTCACAGATATTGCTAGTCCTGCGAATTACATACTCGTTGTTGAGAAAGAATCAGTTTTTCAGCGTCTAGCAAATGACCATTTCTGCAGCAGAAACCGTTGCATTGTAATCTCGGGACGTGGATATCCAGATGTTCCTACTAGAAGGTTTCTGCGGCTTCTCGTTGATGTCTTGGCATTGCCTGCATTTTGTTTGGTAGATTGTGATCCATATGGGTTTGACATTCTGACAACATATCGATTCGGTTCTATGGTTAGGCTCCTCAATTGA

Protein sequence

MAGNLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSEGKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEVTDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLVDCDPYGFDILTTYRFGSMVRLLN

Homology

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BLAST of Carg12869-RA vs. NCBI nr
Match: KAG7024967.1 (Meiotic recombination protein SPO11-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 541.2 bits (1393), Expect = 5.1e-150
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0


Query: 1   MAGNLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60
           MAGNLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSE
Sbjct: 1   MAGNLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60

Query: 61  GKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           GKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN
Sbjct: 61  GKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEV 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEV
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEV 180

Query: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240
           TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP
Sbjct: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240

Query: 241 AFCLVDCDPYGFDILTTYRFGSMVRLLN 269
           AFCLVDCDPYGFDILTTYRFGSMVRLLN
Sbjct: 241 AFCLVDCDPYGFDILTTYRFGSMVRLLN 268

[Close]

BLAST of Carg12869-RA vs. NCBI nr
Match: XP_022976331.1 (meiotic recombination protein SPO11-1 [Cucurbita maxima])

HSP 1 Score: 505.0 bits (1299), Expect = 4.0e-139
Identity = 252/263 (95.82%), Postives = 255/263 (96.96%), Query Frame = 0


Query: 1   MAGNLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60
           MAGNLG SERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYC NSIENCCCRFNLSE
Sbjct: 1   MAGNLGRSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCINSIENCCCRFNLSE 60

Query: 61  GKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           GK+ILTLQ+ESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN
Sbjct: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEV 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRR DCIKKPN     +AHSIPVQVEEV
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRSDCIKKPN-----SAHSIPVQVEEV 180

Query: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240
           TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP
Sbjct: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240

Query: 241 AFCLVDCDPYGFDILTTYRFGSM 264
           AFCLVDCDPYGFDILTTYRFGSM
Sbjct: 241 AFCLVDCDPYGFDILTTYRFGSM 258

[Close]

BLAST of Carg12869-RA vs. NCBI nr
Match: XP_023536168.1 (meiotic recombination protein SPO11-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 501.9 bits (1291), Expect = 3.4e-138
Identity = 251/263 (95.44%), Postives = 254/263 (96.58%), Query Frame = 0


Query: 1   MAGNLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60
           MAG LGSSERIDIMRKIRELTRSMIVEL N SSKMIAIDRFKNYCTNSIENCCCRFNLSE
Sbjct: 1   MAGKLGSSERIDIMRKIRELTRSMIVELCNRSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60

Query: 61  GKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           GK+ILTLQ+ESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN
Sbjct: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEV 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRR DCIKKPN     +AHSIPVQVEEV
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRSDCIKKPN-----SAHSIPVQVEEV 180

Query: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240
           TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP
Sbjct: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240

Query: 241 AFCLVDCDPYGFDILTTYRFGSM 264
           AFCLVDCDPYGFDILTTYRFGSM
Sbjct: 241 AFCLVDCDPYGFDILTTYRFGSM 258

[Close]

BLAST of Carg12869-RA vs. NCBI nr
Match: KAG6592090.1 (Meiotic recombination protein SPO11-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 500.7 bits (1288), Expect = 7.6e-138
Identity = 255/268 (95.15%), Postives = 256/268 (95.52%), Query Frame = 0


Query: 1   MAGNLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60
           MAG LGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNS EN    FNLSE
Sbjct: 1   MAGKLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENW---FNLSE 60

Query: 61  GKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           GK+ILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN
Sbjct: 61  GKEILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEV 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNN     AHSIPVQVEEV
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNN-----AHSIPVQVEEV 180

Query: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240
           TDIASPANYILVVEKESVFQRLANDHFC RNRCIVISGRGYPDVPTRRFLRLLVDVLALP
Sbjct: 181 TDIASPANYILVVEKESVFQRLANDHFCGRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240

Query: 241 AFCLVDCDPYGFDILTTYRFGSMVRLLN 269
           AFCLVDCDPYGFDILTTYRFGSMVRLLN
Sbjct: 241 AFCLVDCDPYGFDILTTYRFGSMVRLLN 260

[Close]

BLAST of Carg12869-RA vs. NCBI nr
Match: XP_022937001.1 (meiotic recombination protein SPO11-1 [Cucurbita moschata])

HSP 1 Score: 500.7 bits (1288), Expect = 7.6e-138
Identity = 251/265 (94.72%), Postives = 254/265 (95.85%), Query Frame = 0


Query: 1   MAGNLGSSE--RIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNL 60
           MAG LGSSE  RIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNS ENCCCRFNL
Sbjct: 1   MAGKLGSSERIRIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENCCCRFNL 60

Query: 61  SEGKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRA 120
           SEGK+ILTLQ++SHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQS VDRA
Sbjct: 61  SEGKEILTLQRQSHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSAVDRA 120

Query: 121 INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVE 180
           INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNN     AHSIPVQVE
Sbjct: 121 INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNN-----AHSIPVQVE 180

Query: 181 EVTDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLA 240
           EVTDIA PANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLA
Sbjct: 181 EVTDIAGPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLA 240

Query: 241 LPAFCLVDCDPYGFDILTTYRFGSM 264
           LPAFCLVDCDPYGFDILTTYRFGSM
Sbjct: 241 LPAFCLVDCDPYGFDILTTYRFGSM 260

[Close]

BLAST of Carg12869-RA vs. ExPASy Swiss-Prot
Match: Q9M4A2 (Meiotic recombination protein SPO11-1 OS=Arabidopsis thaliana OX=3702 GN=SPO11-1 PE=1 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 1.3e-95
Identity = 169/263 (64.26%), Postives = 208/263 (79.09%), Query Frame = 0


Query: 1   MAGNLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60
           M G    SE  +++++I++ T+S++V+L+ G S  I+I++F+NYC N   +C C  +  +
Sbjct: 1   MEGKFAISESTNLLQRIKDFTQSVVVDLAEGRSPKISINQFRNYCMNPEADCLCSSDKPK 60

Query: 61  GKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           G++I TL+KE    R+D+LLRVLLI+QQLL+ENRH+SKRDIYYMHPS F  QS+VDRAI 
Sbjct: 61  GQEIFTLKKEPQTYRIDMLLRVLLIVQQLLQENRHASKRDIYYMHPSAFKAQSIVDRAIG 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEV 180
           DICIL QCSR+NLNVVSVGNGLVMGWL+F EAGR+FDC+   N     TA+ +PV VEEV
Sbjct: 121 DICILFQCSRYNLNVVSVGNGLVMGWLKFREAGRKFDCLNSLN-----TAYPVPVLVEEV 180

Query: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240
            DI S A YILVVEKE+VFQRLAND FC  NRCIVI+GRGYPDV TRRFLRLL++ L LP
Sbjct: 181 EDIVSLAEYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLP 240

Query: 241 AFCLVDCDPYGFDILTTYRFGSM 264
             CLVDCDPYGF+IL TYRFGSM
Sbjct: 241 VHCLVDCDPYGFEILATYRFGSM 258

[Close]

BLAST of Carg12869-RA vs. ExPASy Swiss-Prot
Match: Q5ZPV8 (Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. indica OX=39946 GN=SPO11-1 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 6.6e-84
Identity = 148/260 (56.92%), Postives = 195/260 (75.00%), Query Frame = 0


Query: 9   ERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTN----SIENCCCRFNLSEGKDI 68
           E   ++ +IR L R ++ E++ G S  +A+ R++NYC++    +   C C +++  G D+
Sbjct: 24  EAATLLHRIRGLVRWVVAEVAAGRSPTVALHRYQNYCSSASAAAASPCACSYDVPVGTDV 83

Query: 69  LT-LQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDIC 128
           L+ L + SH  RL+VLLRVLL++QQLL++N+H SKRDIYYM+PS+F +Q+VVDRAINDIC
Sbjct: 84  LSLLHRGSHASRLNVLLRVLLVVQQLLQQNKHCSKRDIYYMYPSIFQEQAVVDRAINDIC 143

Query: 129 ILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEVTDI 188
           +L +CSRHNLNVV V  GLVMGW+RF E  +   C+       V  A SIPV +E + D+
Sbjct: 144 VLFKCSRHNLNVVPVAKGLVMGWIRFLEGEKEVYCV-----TNVNAAFSIPVSIEAIKDV 203

Query: 189 ASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFC 248
            S A+YIL+VEKE+VFQRLAND FC RNRCIVI+GRGYPD+PTRRFLR LV+ L LP +C
Sbjct: 204 VSVADYILIVEKETVFQRLANDKFCERNRCIVITGRGYPDIPTRRFLRYLVEQLHLPVYC 263

Query: 249 LVDCDPYGFDILTTYRFGSM 264
           LVD DPYGFDIL TY+FGS+
Sbjct: 264 LVDADPYGFDILATYKFGSL 278

[Close]

BLAST of Carg12869-RA vs. ExPASy Swiss-Prot
Match: Q7Y021 (Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. japonica OX=39947 GN=SPO11-1 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 6.6e-84
Identity = 148/260 (56.92%), Postives = 195/260 (75.00%), Query Frame = 0


Query: 9   ERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTN----SIENCCCRFNLSEGKDI 68
           E   ++ +IR L R ++ E++ G S  +A+ R++NYC++    +   C C +++  G D+
Sbjct: 24  EAATLLHRIRGLVRWVVAEVAAGRSPTVALHRYQNYCSSASAAAASPCACSYDVPVGTDV 83

Query: 69  LT-LQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDIC 128
           L+ L + SH  RL+VLLRVLL++QQLL++N+H SKRDIYYM+PS+F +Q+VVDRAINDIC
Sbjct: 84  LSLLHRGSHASRLNVLLRVLLVVQQLLQQNKHCSKRDIYYMYPSIFQEQAVVDRAINDIC 143

Query: 129 ILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEVTDI 188
           +L +CSRHNLNVV V  GLVMGW+RF E  +   C+       V  A SIPV +E + D+
Sbjct: 144 VLFKCSRHNLNVVPVAKGLVMGWIRFLEGEKEVYCV-----TNVNAAFSIPVSIEAIKDV 203

Query: 189 ASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALPAFC 248
            S A+YIL+VEKE+VFQRLAND FC RNRCIVI+GRGYPD+PTRRFLR LV+ L LP +C
Sbjct: 204 VSVADYILIVEKETVFQRLANDKFCERNRCIVITGRGYPDIPTRRFLRYLVEQLHLPVYC 263

Query: 249 LVDCDPYGFDILTTYRFGSM 264
           LVD DPYGFDIL TY+FGS+
Sbjct: 264 LVDADPYGFDILATYKFGSL 278

[Close]

BLAST of Carg12869-RA vs. ExPASy Swiss-Prot
Match: Q9Y5K1 (Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 3.9e-36
Identity = 84/262 (32.06%), Postives = 156/262 (59.54%), Query Frame = 0


Query: 12  DIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSEGKDILT----- 71
           +++  I  + + +I  L+   +    ID       +S EN   +F  S G  +++     
Sbjct: 46  EVLASIENIIQDIITSLARNEAPAFTIDN-----RSSWEN--IKFEDSVGLQMVSHCTTR 105

Query: 72  -LQKES--HVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDIC 131
            ++ +S    ++  ++L++L +I +L++ N +++KRDIYY    +F +Q+VVD  INDI 
Sbjct: 106 KIKSDSPKSAQKFSLILKILSMIYKLVQSNTYATKRDIYYTDSQLFGNQTVVDNIINDIS 165

Query: 132 ILLQCSRHNLNVVSVGNGLVMGWLRFAEA-GRRFDCIKKPNNVKVTTAHSIPVQVEEVTD 191
            +L+ SR +L+++S   GL+ G LR+ E  G + +C          TA ++P  ++ + +
Sbjct: 166 CMLKVSRRSLHILSTSKGLIAGNLRYIEEDGTKVNC------TCGATAVAVPSNIQGIRN 225

Query: 192 IASPANYILVVEKESVFQRLANDHFCSR-NRCIVISGRGYPDVPTRRFLRLLVDVLALPA 251
           + + A ++L+VEK++ FQRL +D+FC++ + CI+I+G+G PD+ TR  ++ L D   +P 
Sbjct: 226 LVTDAKFVLIVEKDATFQRLLDDNFCNKLSPCIMITGKGVPDLNTRLLVKKLWDTFHVPV 285

Query: 252 FCLVDCDPYGFDILTTYRFGSM 264
           F LVD DP+G +I+  Y++GSM
Sbjct: 286 FTLVDADPHGIEIMCIYKYGSM 294

[Close]

BLAST of Carg12869-RA vs. ExPASy Swiss-Prot
Match: Q9WTK8 (Meiotic recombination protein SPO11 OS=Mus musculus OX=10090 GN=Spo11 PE=1 SV=2)

HSP 1 Score: 153.3 bits (386), Expect = 3.9e-36
Identity = 75/193 (38.86%), Postives = 126/193 (65.28%), Query Frame = 0


Query: 73  VRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAINDICILLQCSRHN 132
           V++  ++L+VL +I +L++ + +++KRDIYY    +F +Q+ VD AI+DI  +L+  R +
Sbjct: 108 VKKFALILKVLSMIYKLIQSDTYATKRDIYYTDSQLFGNQAAVDSAIDDISCMLKVPRRS 167

Query: 133 LNVVSVGNGLVMGWLRFAEA-GRRFDCIKKPNNVKVTTAHSIPVQVEEVTDIASPANYIL 192
           L+V+S   GL+ G LR+ E  G R  C          TA ++P  ++ +  + + A ++L
Sbjct: 168 LHVLSTSKGLIAGNLRYMEEDGTRVQCTCS------ATATAVPTNIQGMQHLITDAKFLL 227

Query: 193 VVEKESVFQRLANDHFCSR-NRCIVISGRGYPDVPTRRFLRLLVDVLALPAFCLVDCDPY 252
           +VEK++ FQRL +D+FCSR + CI+++G+G PD+ TR  ++ L D   +P F LVD DPY
Sbjct: 228 IVEKDATFQRLLDDNFCSRMSPCIMVTGKGVPDLNTRLLVKKLWDTFHIPVFTLVDADPY 287

Query: 253 GFDILTTYRFGSM 264
           G +I+  Y++GSM
Sbjct: 288 GIEIMCIYKYGSM 294

[Close]

BLAST of Carg12869-RA vs. ExPASy TrEMBL
Match: A0A6J1IGM0 (meiotic recombination protein SPO11-1 OS=Cucurbita maxima OX=3661 GN=LOC111476764 PE=3 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 1.9e-139
Identity = 252/263 (95.82%), Postives = 255/263 (96.96%), Query Frame = 0


Query: 1   MAGNLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60
           MAGNLG SERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYC NSIENCCCRFNLSE
Sbjct: 1   MAGNLGRSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCINSIENCCCRFNLSE 60

Query: 61  GKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           GK+ILTLQ+ESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN
Sbjct: 61  GKEILTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEV 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRR DCIKKPN     +AHSIPVQVEEV
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRSDCIKKPN-----SAHSIPVQVEEV 180

Query: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240
           TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP
Sbjct: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240

Query: 241 AFCLVDCDPYGFDILTTYRFGSM 264
           AFCLVDCDPYGFDILTTYRFGSM
Sbjct: 241 AFCLVDCDPYGFDILTTYRFGSM 258

[Close]

BLAST of Carg12869-RA vs. ExPASy TrEMBL
Match: A0A6J1F921 (meiotic recombination protein SPO11-1 OS=Cucurbita moschata OX=3662 GN=LOC111443423 PE=3 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 3.7e-138
Identity = 251/265 (94.72%), Postives = 254/265 (95.85%), Query Frame = 0


Query: 1   MAGNLGSSE--RIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNL 60
           MAG LGSSE  RIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNS ENCCCRFNL
Sbjct: 1   MAGKLGSSERIRIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSTENCCCRFNL 60

Query: 61  SEGKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRA 120
           SEGK+ILTLQ++SHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQS VDRA
Sbjct: 61  SEGKEILTLQRQSHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSAVDRA 120

Query: 121 INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVE 180
           INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNN     AHSIPVQVE
Sbjct: 121 INDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNN-----AHSIPVQVE 180

Query: 181 EVTDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLA 240
           EVTDIA PANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLA
Sbjct: 181 EVTDIAGPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLA 240

Query: 241 LPAFCLVDCDPYGFDILTTYRFGSM 264
           LPAFCLVDCDPYGFDILTTYRFGSM
Sbjct: 241 LPAFCLVDCDPYGFDILTTYRFGSM 260

[Close]

BLAST of Carg12869-RA vs. ExPASy TrEMBL
Match: A0A1S3CJR6 (meiotic recombination protein SPO11-1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103501238 PE=3 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 8.8e-132
Identity = 235/263 (89.35%), Postives = 250/263 (95.06%), Query Frame = 0


Query: 1   MAGNLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60
           M GN GS ERI+++RKI ELTRSMIVELSNGSSKMIAIDRFK+YCTNS+ENCCCRFNLSE
Sbjct: 1   MEGNRGSLERIEVLRKITELTRSMIVELSNGSSKMIAIDRFKSYCTNSLENCCCRFNLSE 60

Query: 61  GKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           GKD+LTLQ+ESHVRRLDVLLRVLLIIQQLLRENRHSSKRD+YYMHPS+FTDQSVVDRAIN
Sbjct: 61  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDMYYMHPSIFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEV 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRF+EAGRRFDC+KKPN     +A  IPVQVEEV
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPN-----SAQFIPVQVEEV 180

Query: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240
           TDIA+ A+YILVVEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDVLALP
Sbjct: 181 TDIATAADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALP 240

Query: 241 AFCLVDCDPYGFDILTTYRFGSM 264
           AFCLVDCDPYGFDILTTYRFGSM
Sbjct: 241 AFCLVDCDPYGFDILTTYRFGSM 258

[Close]

BLAST of Carg12869-RA vs. ExPASy TrEMBL
Match: A0A6J1CDC7 (meiotic recombination protein SPO11-1 OS=Momordica charantia OX=3673 GN=LOC111010510 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 3.7e-130
Identity = 232/263 (88.21%), Postives = 247/263 (93.92%), Query Frame = 0


Query: 1   MAGNLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60
           M G   SSER DI+R+IRE+TRSMIVELSNGSSKM+AIDRFKNYCTNS+ENCCC+FNLSE
Sbjct: 1   MEGKRASSERSDILRRIREITRSMIVELSNGSSKMMAIDRFKNYCTNSLENCCCKFNLSE 60

Query: 61  GKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           GK+ILTLQKESHVRRLDVLLRVLLI++QLLRENRHSSKRDIYYMHPSVF+DQSVVDRAIN
Sbjct: 61  GKEILTLQKESHVRRLDVLLRVLLIVRQLLRENRHSSKRDIYYMHPSVFSDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEV 180
           DICILLQCSRHNLNVVS GNGLVMGWLRF+EAGRRFDCIKKPN     +AHSIPVQVEEV
Sbjct: 121 DICILLQCSRHNLNVVSFGNGLVMGWLRFSEAGRRFDCIKKPN-----SAHSIPVQVEEV 180

Query: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240
           TDI S A YIL+VEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDV ALP
Sbjct: 181 TDIVSAAEYILIVEKESVFQRLANDQFCSRNRCIVITGRGYPDVPTRRFLRLLVDVSALP 240

Query: 241 AFCLVDCDPYGFDILTTYRFGSM 264
           AFCLVDCDPYGF+ILTTYRFGSM
Sbjct: 241 AFCLVDCDPYGFEILTTYRFGSM 258

[Close]

BLAST of Carg12869-RA vs. ExPASy TrEMBL
Match: A0A1S3CI64 (meiotic recombination protein SPO11-1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103501238 PE=3 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.5e-126
Identity = 231/263 (87.83%), Postives = 246/263 (93.54%), Query Frame = 0


Query: 1   MAGNLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60
           M GN GS ERI+++RKI ELTRSMIVELSNGSSKMIAIDRFK+YCTNS+EN    FNLSE
Sbjct: 1   MEGNRGSLERIEVLRKITELTRSMIVELSNGSSKMIAIDRFKSYCTNSLENW---FNLSE 60

Query: 61  GKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           GKD+LTLQ+ESHVRRLDVLLRVLLIIQQLLRENRHSSKRD+YYMHPS+FTDQSVVDRAIN
Sbjct: 61  GKDVLTLQRESHVRRLDVLLRVLLIIQQLLRENRHSSKRDMYYMHPSIFTDQSVVDRAIN 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEV 180
           DICILLQCSRHNLNVVSVGNGLVMGWLRF+EAGRRFDC+KKPN     +A  IPVQVEEV
Sbjct: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFSEAGRRFDCLKKPN-----SAQFIPVQVEEV 180

Query: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240
           TDIA+ A+YILVVEKESVFQRLAND FCSRNRCIVI+GRGYPDVPTRRFLRLLVDVLALP
Sbjct: 181 TDIATAADYILVVEKESVFQRLANDRFCSRNRCIVITGRGYPDVPTRRFLRLLVDVLALP 240

Query: 241 AFCLVDCDPYGFDILTTYRFGSM 264
           AFCLVDCDPYGFDILTTYRFGSM
Sbjct: 241 AFCLVDCDPYGFDILTTYRFGSM 255

[Close]

BLAST of Carg12869-RA vs. TAIR 10
Match: AT3G13170.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 350.9 bits (899), Expect = 9.1e-97
Identity = 169/263 (64.26%), Postives = 208/263 (79.09%), Query Frame = 0


Query: 1   MAGNLGSSERIDIMRKIRELTRSMIVELSNGSSKMIAIDRFKNYCTNSIENCCCRFNLSE 60
           M G    SE  +++++I++ T+S++V+L+ G S  I+I++F+NYC N   +C C  +  +
Sbjct: 1   MEGKFAISESTNLLQRIKDFTQSVVVDLAEGRSPKISINQFRNYCMNPEADCLCSSDKPK 60

Query: 61  GKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSVVDRAIN 120
           G++I TL+KE    R+D+LLRVLLI+QQLL+ENRH+SKRDIYYMHPS F  QS+VDRAI 
Sbjct: 61  GQEIFTLKKEPQTYRIDMLLRVLLIVQQLLQENRHASKRDIYYMHPSAFKAQSIVDRAIG 120

Query: 121 DICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIPVQVEEV 180
           DICIL QCSR+NLNVVSVGNGLVMGWL+F EAGR+FDC+   N     TA+ +PV VEEV
Sbjct: 121 DICILFQCSRYNLNVVSVGNGLVMGWLKFREAGRKFDCLNSLN-----TAYPVPVLVEEV 180

Query: 181 TDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLVDVLALP 240
            DI S A YILVVEKE+VFQRLAND FC  NRCIVI+GRGYPDV TRRFLRLL++ L LP
Sbjct: 181 EDIVSLAEYILVVEKETVFQRLANDMFCKTNRCIVITGRGYPDVSTRRFLRLLMEKLHLP 240

Query: 241 AFCLVDCDPYGFDILTTYRFGSM 264
             CLVDCDPYGF+IL TYRFGSM
Sbjct: 241 VHCLVDCDPYGFEILATYRFGSM 258

[Close]

BLAST of Carg12869-RA vs. TAIR 10
Match: AT5G02820.1 (Spo11/DNA topoisomerase VI, subunit A protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.8e-37
Identity = 80/208 (38.46%), Postives = 119/208 (57.21%), Query Frame = 0


Query: 55  RFNLSEGKDILTLQKESHVRRLDVLLRVLLIIQQLLRENRHSSKRDIYYMHPSVFTDQSV 114
           R  L +   +      S VR+  +  R+L +I QL   N H +KRD++Y    +F DQ+ 
Sbjct: 122 RIVLKDKSTLRPFASVSSVRKTTITTRILALIHQLCLRNIHVTKRDLFYTDVKLFQDQTQ 181

Query: 115 VDRAINDICILLQCSRHNLNVVSVGNGLVMGWLRFAEAGRRFDCIKKPNNVKVTTAHSIP 174
            D  ++D+  +L C+R +LNV++   G+V+G L F++ G   DC K     K     +IP
Sbjct: 182 SDAVLDDVSCMLGCTRSSLNVIAAEKGVVVGRLIFSDNGDMIDCTKMGMGGK-----AIP 241

Query: 175 VQVEEVTDIASPANYILVVEKESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFLRLLV 234
             ++ V D+ S A +IL+VEK++ + RLA D F +R  CI+++ +G PDV TR FLR + 
Sbjct: 242 PNIDRVGDMQSDAMFILLVEKDAAYMRLAEDRFYNRFPCIIVTAKGQPDVATRLFLRKMK 301

Query: 235 DVLALPAFCLVDCDPYGFDILTTYRFGS 263
             L LP   LVD DPYG  IL+ Y  GS
Sbjct: 302 MELKLPVLALVDSDPYGLKILSVYGCGS 324

[Close]

BLAST of Carg12869-RA vs. TAIR 10
Match: AT1G63990.1 (sporulation 11-2 )

HSP 1 Score: 109.8 bits (273), Expect = 3.5e-24
Identity = 63/189 (33.33%), Postives = 106/189 (56.08%), Query Frame = 0


Query: 81  RVLLIIQQLLRENRHSSKRDIYYM----HPSVFTDQSVVDRAINDICILLQCSRHNLNVV 140
           +V+ I  Q+L + +  ++R+++Y      P  F+ Q  V+R++ D+  LL+CSR++L ++
Sbjct: 102 KVMEICFQILLQEKRVTQRELFYKLLCDSPDYFSSQIEVNRSVQDVVALLRCSRYSLGIM 161

Query: 141 SVGNGLVMGWLRFAEAGRR-FDCIKKPNNVKVTTAHSIPVQVEEVTDIASPANYILVVEK 200
           +   GLV G L   E G+   DC    ++    T     + + + T + + A YI++VEK
Sbjct: 162 ASSRGLVAGRLFLQEPGKEAVDCSACGSSGFAITG---DLNLLDNTIMRTDARYIIIVEK 221

Query: 201 ESVFQRLANDHFCSRNRCIVISGRGYPDVPTRRFL-RLLVDVLALPAFCLVDCDPYGFDI 260
            ++F RL  D   +   C+ I+ +GYPD+ TR FL R+      LP   LVD +P G  I
Sbjct: 222 HAIFHRLVEDRVFNHIPCVFITAKGYPDIATRFFLHRMSTTFPDLPILVLVDWNPAGLAI 281

Query: 261 LTTYRFGSM 264
           L T++FGS+
Sbjct: 282 LCTFKFGSI 287

The following BLAST results are available for this feature:

BLAST of Carg12869-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7024967.1	5.1e-150	100.00	Meiotic recombination protein SPO11-1, partial [Cucurbita argyrosperma subsp. ar...	[more]
XP_022976331.1	4.0e-139	95.82	meiotic recombination protein SPO11-1 [Cucurbita maxima]	[more]
XP_023536168.1	3.4e-138	95.44	meiotic recombination protein SPO11-1 [Cucurbita pepo subsp. pepo]	[more]
KAG6592090.1	7.6e-138	95.15	Meiotic recombination protein SPO11-1, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022937001.1	7.6e-138	94.72	meiotic recombination protein SPO11-1 [Cucurbita moschata]	[more]

BLAST of Carg12869-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M4A2	1.3e-95	64.26	Meiotic recombination protein SPO11-1 OS=Arabidopsis thaliana OX=3702 GN=SPO11-1...	[more]
Q5ZPV8	6.6e-84	56.92	Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. indica OX=39946 GN=...	[more]
Q7Y021	6.6e-84	56.92	Meiotic recombination protein SPO11-1 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
Q9Y5K1	3.9e-36	32.06	Meiotic recombination protein SPO11 OS=Homo sapiens OX=9606 GN=SPO11 PE=1 SV=1	[more]
Q9WTK8	3.9e-36	38.86	Meiotic recombination protein SPO11 OS=Mus musculus OX=10090 GN=Spo11 PE=1 SV=2	[more]

BLAST of Carg12869-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IGM0	1.9e-139	95.82	meiotic recombination protein SPO11-1 OS=Cucurbita maxima OX=3661 GN=LOC11147676...	[more]
A0A6J1F921	3.7e-138	94.72	meiotic recombination protein SPO11-1 OS=Cucurbita moschata OX=3662 GN=LOC111443...	[more]
A0A1S3CJR6	8.8e-132	89.35	meiotic recombination protein SPO11-1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A6J1CDC7	3.7e-130	88.21	meiotic recombination protein SPO11-1 OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A1S3CI64	2.5e-126	87.83	meiotic recombination protein SPO11-1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC1...	[more]

BLAST of Carg12869-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G13170.1	9.1e-97	64.26	Spo11/DNA topoisomerase VI, subunit A protein	[more]
AT5G02820.1	2.8e-37	38.46	Spo11/DNA topoisomerase VI, subunit A protein	[more]
AT1G63990.1	3.5e-24	33.33	sporulation 11-2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002815	Spo11/DNA topoisomerase VI subunit A	PRINTS	PR01550	TOP6AFAMILY	coord: 130..145 score: 41.41 coord: 98..109 score: 50.0 coord: 112..129 score: 43.06 coord: 214..235 score: 42.9 coord: 246..262 score: 52.57 coord: 190..207 score: 54.17
IPR002815	Spo11/DNA topoisomerase VI subunit A	PANTHER	PTHR10848	MEIOTIC RECOMBINATION PROTEIN SPO11	coord: 8..264
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 4..138 e-value: 1.8E-22 score: 81.7
IPR013049	Spo11/DNA topoisomerase VI, subunit A, N-terminal	PFAM	PF04406	TP6A_N	coord: 74..135 e-value: 4.0E-22 score: 78.0
None	No IPR available	GENE3D	3.40.1360.10		coord: 140..267 e-value: 2.5E-45 score: 156.7
None	No IPR available	PANTHER	PTHR10848:SF3	MEIOTIC RECOMBINATION PROTEIN SPO11-1	coord: 8..264
IPR034136	Topoisomerase 6 subunit A/Spo11, TOPRIM domain	CDD	cd00223	TOPRIM_TopoIIB_SPO	coord: 188..263 e-value: 4.63891E-38 score: 129.297
IPR036078	Spo11/DNA topoisomerase VI subunit A superfamily	SUPERFAMILY	56726	DNA topoisomerase IV, alpha subunit	coord: 73..263

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg12869	Carg12869	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12869-RA:1	Carg12869-RA:1	exon
Carg12869-RA:2	Carg12869-RA:2	exon
Carg12869-RA:3	Carg12869-RA:3	exon
Carg12869-RA:4	Carg12869-RA:4	exon
Carg12869-RA:5	Carg12869-RA:5	exon
Carg12869-RA:6	Carg12869-RA:6	exon
Carg12869-RA:7	Carg12869-RA:7	exon
Carg12869-RA:8	Carg12869-RA:8	exon
Carg12869-RA:9	Carg12869-RA:9	exon
Carg12869-RA:10	Carg12869-RA:10	exon
Carg12869-RA:11	Carg12869-RA:11	exon
Carg12869-RA:12	Carg12869-RA:12	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12869-RA:cds	Carg12869-RA:cds	CDS
Carg12869-RA:cds	Carg12869-RA:cds_2	CDS
Carg12869-RA:cds	Carg12869-RA:cds_3	CDS
Carg12869-RA:cds	Carg12869-RA:cds_4	CDS
Carg12869-RA:cds	Carg12869-RA:cds_5	CDS
Carg12869-RA:cds	Carg12869-RA:cds_6	CDS
Carg12869-RA:cds	Carg12869-RA:cds_7	CDS
Carg12869-RA:cds	Carg12869-RA:cds_8	CDS
Carg12869-RA:cds	Carg12869-RA:cds_9	CDS
Carg12869-RA:cds	Carg12869-RA:cds_10	CDS
Carg12869-RA:cds	Carg12869-RA:cds_11	CDS
Carg12869-RA:cds	Carg12869-RA:cds_12	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg12869-RA	Carg12869-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006259	DNA metabolic process
biological_process	GO:0042138	meiotic DNA double-strand break formation
biological_process	GO:0051026	chiasma assembly
biological_process	GO:0000724	double-strand break repair via homologous recombination
cellular_component	GO:0005694	chromosome
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003918	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0046872	metal ion binding