Carg12512-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12512-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Adenine nucleotide alpha hydrolases-like superfamily protein
Location	Carg_Chr14: 446611 .. 447892 (+)
Sequence length	1027
RNA-Seq Expression	Carg12512-RA
Synteny	Carg12512-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAACTTCAGGTCCGCGTTCTTACTTCCCTTGCGTTTTCGTTTTAATCGCACTGTTCCCATTTCACGAAAAGAGAAGAAACGCCACAAAAATGGTGAAGGATCGGAAGATCGGAGTGGCTCTGGATTTCTCCAGCAGCAGCAAGAATGCTCTGAGATGGGCCATCGATAATTTGGCAGACAAAGACGACACTCTCTTCATCATCTACGTTAACCCCAATTCAGTTAACGAATCCCCCCATAAGCTCTGGGCTGAATCTGGTTCTCGTCAGTAATCGATCCTCTTTTTTCTTTTGATGTTTTCTCATACTCACTTTCTGTAGATCTTGATGATTTGTTTGGGTTTTTGCCAGCTCTGATTCCCTTATCGGAATTCAGAGAGCCTGATGTTTTGAAGAGCTATGATGTCAAAGTCGACATCGAAGCCCTCGATATCCTCGACACTGGCTCTAGACAGAAAGAGGTACTCACTTTGATTTTGAGCTCTAGATTTTTGATGAAAGGTAAAGTAAAGTGCGATTTCAGATTCAAGTTGTGGCGAAACTGTACTGGGGAGATGCGAGGGAGAAGATTGTGGATGCAATCGAAGATCTGAAGCTGGAGTGTCTGGTAATGGGAAGCAGAGGACAGAGTACCATTCGAAGGTTTGTCTTCTTTTTCTTCTTCTGATAGTGAATCGTTTATTGGAAATGATGAACAATTGTTTACGTATTTATTGGGGAAATTTTGTGTTTGGGTGATCGGAAAACAGGATTCTGCTTGGGAGTGTTAGCAACTATGTGATTACTCATGCTCCTTGCCCTGTTACAGTGGTGAAGGATTCAAAATTTCAGCATAATTAAGGCGTGAAATGGGTGATAGGAGGGGGGTTTTTTGTTGTTTGATGATTAAGAGCATGCCCAAACTCCTGAAGTTGTAGCAAATGCTGTTTGTGTCCTTGAAAACGTTGCTCCTCTCCCTGCGCTCTCATATTTAATAATTGCCCCTACCCACACCCACACCCACACCCACACCCATACCCACACTCATCCGAGATCTTCTTCTGGACTGCGCCGCGCCCTGTTTATGTTTCTTTTCTGAAAACATTATTATTATTATTATTACAATGATGCTTTTTAAGAACTAAACTACTTCGTTTCGTTTATTTATTTGATTGGTTGATTTGTTCCAAGTATAAGCGGTGGATAGAATTATTCCTTGGATTCCGAAAATTTCTTTTTATTCCTTTGAAAAATTTAAGGTTATCCTGAATTTGACATAATCAACTGGATTTGGTTGTGTTTC

mRNA sequence

GCAACTTCAGGTCCGCGTTCTTACTTCCCTTGCGTTTTCGTTTTAATCGCACTGTTCCCATTTCACGAAAAGAGAAGAAACGCCACAAAAATGGTGAAGGATCGGAAGATCGGAGTGGCTCTGGATTTCTCCAGCAGCAGCAAGAATGCTCTGAGATGGGCCATCGATAATTTGGCAGACAAAGACGACACTCTCTTCATCATCTACGTTAACCCCAATTCAGTTAACGAATCCCCCCATAAGCTCTGGGCTGAATCTGGTTCTCCTCTGATTCCCTTATCGGAATTCAGAGAGCCTGATGTTTTGAAGAGCTATGATGTCAAAGTCGACATCGAAGCCCTCGATATCCTCGACACTGGCTCTAGACAGAAAGAGATTCAAGTTGTGGCGAAACTGTACTGGGGAGATGCGAGGGAGAAGATTGTGGATGCAATCGAAGATCTGAAGCTGGAGTGTCTGGTAATGGGAAGCAGAGGACAGAGTACCATTCGAAGGATTCTGCTTGGGAGTGTTAGCAACTATGTGATTACTCATGCTCCTTGCCCTGTTACAGTGGTGAAGGATTCAAAATTTCAGCATAATTAAGGCGTGAAATGGGTGATAGGAGGGGGGTTTTTTGTTGTTTGATGATTAAGAGCATGCCCAAACTCCTGAAGTTGTAGCAAATGCTGTTTGTGTCCTTGAAAACGTTGCTCCTCTCCCTGCGCTCTCATATTTAATAATTGCCCCTACCCACACCCACACCCACACCCACACCCATACCCACACTCATCCGAGATCTTCTTCTGGACTGCGCCGCGCCCTGTTTATGTTTCTTTTCTGAAAACATTATTATTATTATTATTACAATGATGCTTTTTAAGAACTAAACTACTTCGTTTCGTTTATTTATTTGATTGGTTGATTTGTTCCAAGTATAAGCGGTGGATAGAATTATTCCTTGGATTCCGAAAATTTCTTTTTATTCCTTTGAAAAATTTAAGGTTATCCTGAATTTGACATAATCAACTGGATTTGGTTGTGTTTC

Coding sequence (CDS)

ATGGTGAAGGATCGGAAGATCGGAGTGGCTCTGGATTTCTCCAGCAGCAGCAAGAATGCTCTGAGATGGGCCATCGATAATTTGGCAGACAAAGACGACACTCTCTTCATCATCTACGTTAACCCCAATTCAGTTAACGAATCCCCCCATAAGCTCTGGGCTGAATCTGGTTCTCCTCTGATTCCCTTATCGGAATTCAGAGAGCCTGATGTTTTGAAGAGCTATGATGTCAAAGTCGACATCGAAGCCCTCGATATCCTCGACACTGGCTCTAGACAGAAAGAGATTCAAGTTGTGGCGAAACTGTACTGGGGAGATGCGAGGGAGAAGATTGTGGATGCAATCGAAGATCTGAAGCTGGAGTGTCTGGTAATGGGAAGCAGAGGACAGAGTACCATTCGAAGGATTCTGCTTGGGAGTGTTAGCAACTATGTGATTACTCATGCTCCTTGCCCTGTTACAGTGGTGAAGGATTCAAAATTTCAGCATAATTAA

Protein sequence

MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPLIPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKLECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN

Homology

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BLAST of Carg12512-RA vs. NCBI nr
Match: KAG6580510.1 (Universal stress protein PHOS32, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017259.1 Universal stress protein PHOS32 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 330.5 bits (846), Expect = 8.3e-87
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60
           MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL
Sbjct: 1   MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60

Query: 61  IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 120
           IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL
Sbjct: 61  IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 120

Query: 121 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN 165
           ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN
Sbjct: 121 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN 164

[Close]

BLAST of Carg12512-RA vs. NCBI nr
Match: XP_022935543.1 (universal stress protein PHOS32-like [Cucurbita moschata])

HSP 1 Score: 326.2 bits (835), Expect = 1.6e-85
Identity = 162/164 (98.78%), Postives = 163/164 (99.39%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60
           MVKDRKIGVALDFSSSSKNALRWAIDNLADK DTLFIIYVNPNSVNESPHKLWAESGSPL
Sbjct: 50  MVKDRKIGVALDFSSSSKNALRWAIDNLADKGDTLFIIYVNPNSVNESPHKLWAESGSPL 109

Query: 61  IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 120
           IPLSEFREPDVLKSYDV+VDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL
Sbjct: 110 IPLSEFREPDVLKSYDVEVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 169

Query: 121 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN 165
           ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN
Sbjct: 170 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN 213

[Close]

BLAST of Carg12512-RA vs. NCBI nr
Match: XP_023526838.1 (universal stress protein PHOS32-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 324.3 bits (830), Expect = 6.0e-85
Identity = 160/164 (97.56%), Postives = 163/164 (99.39%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60
           MVKDRKIGVALDFSSSSKNALRWAIDNLADK DTLFIIYVNPNSVNESPHKLWAESGSPL
Sbjct: 50  MVKDRKIGVALDFSSSSKNALRWAIDNLADKGDTLFIIYVNPNSVNESPHKLWAESGSPL 109

Query: 61  IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 120
           IPLSEFR+PDVLKSYDVKVDIEALDILDTGSRQKEIQVV+KLYWGDAREKIVDAIEDLKL
Sbjct: 110 IPLSEFRQPDVLKSYDVKVDIEALDILDTGSRQKEIQVVSKLYWGDAREKIVDAIEDLKL 169

Query: 121 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN 165
           ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKF+HN
Sbjct: 170 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFEHN 213

[Close]

BLAST of Carg12512-RA vs. NCBI nr
Match: XP_022983606.1 (universal stress protein PHOS32-like [Cucurbita maxima])

HSP 1 Score: 317.4 bits (812), Expect = 7.3e-83
Identity = 156/164 (95.12%), Postives = 161/164 (98.17%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60
           MVKDRKIGVALDFS SSKNALRWAIDNLADK DTLFIIYVNPNSV ESPH+LWAESGSPL
Sbjct: 50  MVKDRKIGVALDFSRSSKNALRWAIDNLADKGDTLFIIYVNPNSVTESPHQLWAESGSPL 109

Query: 61  IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 120
           IPLSEFR+PDVLKSYDVKVDIEALDILDTG+RQKEIQVV+KLYWGDAREKIVDAIEDLKL
Sbjct: 110 IPLSEFRQPDVLKSYDVKVDIEALDILDTGARQKEIQVVSKLYWGDAREKIVDAIEDLKL 169

Query: 121 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN 165
           ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKF+HN
Sbjct: 170 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFEHN 213

[Close]

BLAST of Carg12512-RA vs. NCBI nr
Match: XP_038903504.1 (universal stress protein PHOS32 [Benincasa hispida])

HSP 1 Score: 297.7 bits (761), Expect = 6.0e-77
Identity = 146/164 (89.02%), Postives = 156/164 (95.12%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60
           MVKDRKIGVALDFS+SSKNALRWAIDNLADK DTLFIIYVNPNS++ES H+LWAESGSPL
Sbjct: 1   MVKDRKIGVALDFSNSSKNALRWAIDNLADKGDTLFIIYVNPNSLDESAHRLWAESGSPL 60

Query: 61  IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 120
           IPLSEFREPDVLK YDVK+DIEALD LDTG+RQKEI VV+KLYWGDAREKIVDAIEDLKL
Sbjct: 61  IPLSEFREPDVLKKYDVKIDIEALDTLDTGARQKEITVVSKLYWGDAREKIVDAIEDLKL 120

Query: 121 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN 165
           + LVMGSRG STIRRILLGSVSNYVITHAPCPVTVVK+S FQH+
Sbjct: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKESNFQHH 164

[Close]

BLAST of Carg12512-RA vs. ExPASy Swiss-Prot
Match: Q8VYN9 (Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 4.8e-13
Identity = 53/163 (32.52%), Postives = 83/163 (50.92%), Query Frame = 0


Query: 5   RKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPLIPLS 64
           RKIGVA+D S  S  A+RWA+D+     D + +++V+P SV      L+     PL   +
Sbjct: 48  RKIGVAVDLSEESSFAVRWAVDHYIRPGDAVVLLHVSPTSV------LFGADWGPLPLKT 107

Query: 65  EFREPDVLKSYDVKVDIEAL---DILDTGSRQKEIQVVAKLYW---GDAREKIVDAIEDL 124
           +  +P+  +    + D +A     + D     KE+    K++     D RE++   IE L
Sbjct: 108 QIEDPNA-QPQPSQEDFDAFTSTKVADLAKPLKELGFPYKIHIVKDHDMRERLCLEIERL 167

Query: 125 KLECLVMGSRGQSTIRRI----LLGSVSNYVITHAPCPVTVVK 158
            L  ++MGSRG    ++      LGSVS+Y + H  CPV VV+
Sbjct: 168 GLSAVIMGSRGFGAEKKRGSDGKLGSVSDYCVHHCVCPVVVVR 203

[Close]

BLAST of Carg12512-RA vs. ExPASy Swiss-Prot
Match: Q8L4N1 (Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.7e-11
Identity = 56/167 (33.53%), Postives = 79/167 (47.31%), Query Frame = 0


Query: 5   RKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPLIPLS 64
           RKIGVA+D S  S  A+RWA+D+     D + I++V+P SV      L+     PL PL 
Sbjct: 45  RKIGVAVDLSEESAFAVRWAVDHYIRPGDAVVILHVSPTSV------LFGADWGPL-PLQ 104

Query: 65  EFREPDV-----LKSYDVKVDIEAL---DILDTGSRQKEIQVVAKLYW---GDAREKIVD 124
               P        +    + D +A     + D     KE     K++     D RE++  
Sbjct: 105 TPPPPSAATDPGAQPKPSQEDFDAFTSSKVADLAKPLKEAGFPHKIHIVKDHDMRERLCL 164

Query: 125 AIEDLKLECLVMGSRGQSTIRR---ILLGSVSNYVITHAPCPVTVVK 158
             E L L  ++MGSRG    +R     LGSVS+Y + H  CPV VV+
Sbjct: 165 ETERLNLSAVIMGSRGFGAEKRGSDGKLGSVSDYCVHHCVCPVVVVR 204

[Close]

BLAST of Carg12512-RA vs. ExPASy Swiss-Prot
Match: Q57951 (Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0531 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 6.1e-08
Identity = 51/157 (32.48%), Postives = 80/157 (50.96%), Query Frame = 0


Query: 5   RKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPH-KLWAESGSPLIPL 64
           +KI +  D S  S  A + AI+   + D  ++ IYV    V+ SP   L AE    LI  
Sbjct: 27  KKIVIPTDGSDVSLEAAKHAINIAKEFDAEVYAIYV----VDVSPFVGLPAEGSWELI-- 86

Query: 65  SEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKLECL 124
                 ++LK    +   EAL  +   + +  +++  ++  G    +IV+  E  K + +
Sbjct: 87  -----SELLK----EEGQEALKKVKKMAEEWGVKIHTEMLEGVPANEIVEFAEKKKADLI 146

Query: 125 VMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSK 161
           VMG+ G++ + RILLGSV+  VI +A CPV VVK  K
Sbjct: 147 VMGTTGKTGLERILLGSVAERVIKNAHCPVLVVKKPK 168

[Close]

BLAST of Carg12512-RA vs. ExPASy Swiss-Prot
Match: P87132 (Uncharacterized protein C167.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC167.05 PE=1 SV=2)

HSP 1 Score: 57.4 bits (137), Expect = 1.8e-07
Identity = 42/150 (28.00%), Postives = 74/150 (49.33%), Query Frame = 0


Query: 9   VALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPLI-PLSEFR 68
           + LD SS S +A  WA+  L    DTL I+ V              E   P    + +  
Sbjct: 435 LTLDLSSESLHAAEWAVGILLRNGDTLIIVDV-------------IECDDPSARAVKDRM 494

Query: 69  EPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKLECLVMGS 128
           E + L++ + K+    L +L     + E+ +   ++   A+  I++ I+ ++   +VMGS
Sbjct: 495 ESEQLETLE-KITKYILKLLSKTVLEVEVNIEV-IHHEKAKHLIIEMIDYIEPSLVVMGS 554

Query: 129 RGQSTIRRILLGSVSNYVITHAPCPVTVVK 158
           RG+S ++ +LLGS SNY++  +  PV V +
Sbjct: 555 RGRSHLKGVLLGSFSNYLVNKSSVPVMVAR 569

[Close]

BLAST of Carg12512-RA vs. ExPASy Swiss-Prot
Match: Q57997 (Universal stress protein MJ0577 OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=MJ0577 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 4.9e-05
Identity = 32/85 (37.65%), Postives = 49/85 (57.65%), Query Frame = 0


Query: 73  KSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKLECLVMGSRGQST 132
           K  ++K ++E     D G + K+I VV     G   E+IV   ED  ++ ++MGS G++ 
Sbjct: 84  KMENIKKELE-----DVGFKVKDIIVV-----GIPHEEIVKIAEDEGVDIIIMGSHGKTN 143

Query: 133 IRRILLGSVSNYVITHAPCPVTVVK 158
           ++ ILLGSV+  VI  +  PV VVK
Sbjct: 144 LKEILLGSVTENVIKKSNKPVLVVK 158

[Close]

BLAST of Carg12512-RA vs. ExPASy TrEMBL
Match: A0A6J1FAU1 (universal stress protein PHOS32-like OS=Cucurbita moschata OX=3662 GN=LOC111442383 PE=4 SV=1)

HSP 1 Score: 326.2 bits (835), Expect = 7.6e-86
Identity = 162/164 (98.78%), Postives = 163/164 (99.39%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60
           MVKDRKIGVALDFSSSSKNALRWAIDNLADK DTLFIIYVNPNSVNESPHKLWAESGSPL
Sbjct: 50  MVKDRKIGVALDFSSSSKNALRWAIDNLADKGDTLFIIYVNPNSVNESPHKLWAESGSPL 109

Query: 61  IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 120
           IPLSEFREPDVLKSYDV+VDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL
Sbjct: 110 IPLSEFREPDVLKSYDVEVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 169

Query: 121 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN 165
           ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN
Sbjct: 170 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN 213

[Close]

BLAST of Carg12512-RA vs. ExPASy TrEMBL
Match: A0A6J1J2T3 (universal stress protein PHOS32-like OS=Cucurbita maxima OX=3661 GN=LOC111482162 PE=4 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 3.5e-83
Identity = 156/164 (95.12%), Postives = 161/164 (98.17%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60
           MVKDRKIGVALDFS SSKNALRWAIDNLADK DTLFIIYVNPNSV ESPH+LWAESGSPL
Sbjct: 50  MVKDRKIGVALDFSRSSKNALRWAIDNLADKGDTLFIIYVNPNSVTESPHQLWAESGSPL 109

Query: 61  IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 120
           IPLSEFR+PDVLKSYDVKVDIEALDILDTG+RQKEIQVV+KLYWGDAREKIVDAIEDLKL
Sbjct: 110 IPLSEFRQPDVLKSYDVKVDIEALDILDTGARQKEIQVVSKLYWGDAREKIVDAIEDLKL 169

Query: 121 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN 165
           ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKF+HN
Sbjct: 170 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFEHN 213

[Close]

BLAST of Carg12512-RA vs. ExPASy TrEMBL
Match: A0A0A0LDK3 (Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G733270 PE=4 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 4.9e-77
Identity = 146/164 (89.02%), Postives = 155/164 (94.51%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60
           MVKDRKIGVALDFS+SSKNALRWAIDNLADK DTLFIIYVNPNS+ ES H+LWAESGSPL
Sbjct: 1   MVKDRKIGVALDFSNSSKNALRWAIDNLADKGDTLFIIYVNPNSLEESAHRLWAESGSPL 60

Query: 61  IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 120
           IPLSEFREP+VLK YDVK+DIEALDILDTG+RQKEI VV+KLYWGDAREKIVDAIEDLKL
Sbjct: 61  IPLSEFREPEVLKKYDVKIDIEALDILDTGARQKEITVVSKLYWGDAREKIVDAIEDLKL 120

Query: 121 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN 165
           + LVMGSRG STIRRILLGSVSNYVITHAPCPVTVVKDS F H+
Sbjct: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKDSNFPHH 164

[Close]

BLAST of Carg12512-RA vs. ExPASy TrEMBL
Match: A0A5A7TSD0 (Universal stress protein PHOS32-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001270 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.4e-76
Identity = 144/164 (87.80%), Postives = 155/164 (94.51%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60
           MVKDRKIGVALDFS SSKNAL+WAIDNLADK DTL+IIYVNPNS+NES H+LWAESGSPL
Sbjct: 1   MVKDRKIGVALDFSDSSKNALKWAIDNLADKGDTLYIIYVNPNSLNESAHRLWAESGSPL 60

Query: 61  IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 120
           IPLSEFREP+VLK YDVK+DI+ALDILDTG+RQKEI VV+KLYWGDAREKIVDAIEDLKL
Sbjct: 61  IPLSEFREPEVLKKYDVKIDIDALDILDTGARQKEITVVSKLYWGDAREKIVDAIEDLKL 120

Query: 121 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN 165
           + LVMGSRG STIRRILLGSVSNYVITHAPCPVTVVKDS F H+
Sbjct: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKDSNFPHH 164

[Close]

BLAST of Carg12512-RA vs. ExPASy TrEMBL
Match: A0A1S3B5T0 (uncharacterized protein C167.05 OS=Cucumis melo OX=3656 GN=LOC103486344 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.4e-76
Identity = 144/164 (87.80%), Postives = 155/164 (94.51%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60
           MVKDRKIGVALDFS SSKNAL+WAIDNLADK DTL+IIYVNPNS+NES H+LWAESGSPL
Sbjct: 1   MVKDRKIGVALDFSDSSKNALKWAIDNLADKGDTLYIIYVNPNSLNESAHRLWAESGSPL 60

Query: 61  IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 120
           IPLSEFREP+VLK YDVK+DI+ALDILDTG+RQKEI VV+KLYWGDAREKIVDAIEDLKL
Sbjct: 61  IPLSEFREPEVLKKYDVKIDIDALDILDTGARQKEITVVSKLYWGDAREKIVDAIEDLKL 120

Query: 121 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSKFQHN 165
           + LVMGSRG STIRRILLGSVSNYVITHAPCPVTVVKDS F H+
Sbjct: 121 DSLVMGSRGLSTIRRILLGSVSNYVITHAPCPVTVVKDSNFPHH 164

[Close]

BLAST of Carg12512-RA vs. TAIR 10
Match: AT3G53990.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 233.8 bits (595), Expect = 9.9e-62
Identity = 108/160 (67.50%), Postives = 138/160 (86.25%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60
           M KDR IG+A+DFS SSKNAL+WAI+NLADK DT++II+  P S +ES + LW +SGSPL
Sbjct: 1   MPKDRNIGIAMDFSESSKNALKWAIENLADKGDTIYIIHTLPLSGDESRNSLWFKSGSPL 60

Query: 61  IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 120
           IPL+EFREP++++ Y VK DI  LD+LDTGSRQKE+ VV KLYWGDAREK+VDA++DLKL
Sbjct: 61  IPLAEFREPEIMEKYGVKTDIACLDMLDTGSRQKEVHVVTKLYWGDAREKLVDAVKDLKL 120

Query: 121 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVKDSK 161
           + +VMGSRG S ++RI++GSVS++VI HAPCPVTVVKD++
Sbjct: 121 DSIVMGSRGLSALQRIIMGSVSSFVIQHAPCPVTVVKDNE 160

[Close]

BLAST of Carg12512-RA vs. TAIR 10
Match: AT3G03270.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 183.3 bits (464), Expect = 1.5e-46
Identity = 87/157 (55.41%), Postives = 121/157 (77.07%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60
           M K R +GV +D+S +SK ALRWA +NL +  DT+ +I+V P + + +   L+ E+GSPL
Sbjct: 1   MGKARTVGVGMDYSPTSKLALRWAAENLLEDGDTVILIHVQPQNADHTRKILFEETGSPL 60

Query: 61  IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 120
           IPL EFRE ++ K Y +  D E LD+LDT SR K+++VVAK+YWGD REK+ DA+E+LKL
Sbjct: 61  IPLEEFREVNLSKQYGLAYDPEVLDVLDTLSRAKKVKVVAKVYWGDPREKLCDAVENLKL 120

Query: 121 ECLVMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVK 158
           + +V+GSRG  +++RILLGSVSN+V+T+A CPVTVVK
Sbjct: 121 DSIVLGSRGLGSLKRILLGSVSNHVVTNATCPVTVVK 157

[Close]

BLAST of Carg12512-RA vs. TAIR 10
Match: AT3G17020.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 157.5 bits (397), Expect = 9.0e-39
Identity = 78/154 (50.65%), Postives = 104/154 (67.53%), Query Frame = 0


Query: 5   RKIGVALDFSSSSKNALRWAIDNLA-DKDDTLFIIYVNPNSVNESPHKLWAESGSPLIPL 64
           R+IGVA+DFS  SK AL WAIDN+  D D  + I   +  +  E   +LW   GSP IP+
Sbjct: 7   RRIGVAVDFSDCSKKALSWAIDNVVRDGDHLILITIAHDMNYEEGEMQLWETVGSPFIPM 66

Query: 65  SEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKLECL 124
           SEF +  V+K Y +K D E LDI++T +R+K I VV K+YWGD REKI  A E + L  L
Sbjct: 67  SEFSDAAVMKKYALKPDAETLDIVNTAARKKTITVVMKIYWGDPREKICAAAEQIPLSSL 126

Query: 125 VMGSRGQSTIRRILLGSVSNYVITHAPCPVTVVK 158
           VMG+RG   ++R+++GSVSN+V+ +  CPVTVVK
Sbjct: 127 VMGNRGLGGLKRMIMGSVSNHVVNNVACPVTVVK 160

[Close]

BLAST of Carg12512-RA vs. TAIR 10
Match: AT3G03270.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 148.3 bits (373), Expect = 5.5e-36
Identity = 69/135 (51.11%), Postives = 100/135 (74.07%), Query Frame = 0


Query: 1   MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60
           M K R +GV +D+S +SK ALRWA +NL +  DT+ +I+V P + + +   L+ E+GSPL
Sbjct: 1   MGKARTVGVGMDYSPTSKLALRWAAENLLEDGDTVILIHVQPQNADHTRKILFEETGSPL 60

Query: 61  IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEIQVVAKLYWGDAREKIVDAIEDLKL 120
           IPL EFRE ++ K Y +  D E LD+LDT SR K+++VVAK+YWGD REK+ DA+E+LKL
Sbjct: 61  IPLEEFREVNLSKQYGLAYDPEVLDVLDTLSRAKKVKVVAKVYWGDPREKLCDAVENLKL 120

Query: 121 ECLVMGSRGQSTIRR 136
           + +V+GSRG  +++R
Sbjct: 121 DSIVLGSRGLGSLKR 135

[Close]

BLAST of Carg12512-RA vs. TAIR 10
Match: AT3G53990.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 136.3 bits (342), Expect = 2.1e-32
Identity = 63/96 (65.62%), Postives = 80/96 (83.33%), Query Frame = 0


Query: 1  MVKDRKIGVALDFSSSSKNALRWAIDNLADKDDTLFIIYVNPNSVNESPHKLWAESGSPL 60
          M KDR IG+A+DFS SSKNAL+WAI+NLADK DT++II+  P S +ES + LW +SGSPL
Sbjct: 1  MPKDRNIGIAMDFSESSKNALKWAIENLADKGDTIYIIHTLPLSGDESRNSLWFKSGSPL 60

Query: 61 IPLSEFREPDVLKSYDVKVDIEALDILDTGSRQKEI 97
          IPL+EFREP++++ Y VK DI  LD+LDTGSRQKE+
Sbjct: 61 IPLAEFREPEIMEKYGVKTDIACLDMLDTGSRQKEV 96

The following BLAST results are available for this feature:

BLAST of Carg12512-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6580510.1	8.3e-87	100.00	Universal stress protein PHOS32, partial [Cucurbita argyrosperma subsp. sororia]...	[more]
XP_022935543.1	1.6e-85	98.78	universal stress protein PHOS32-like [Cucurbita moschata]	[more]
XP_023526838.1	6.0e-85	97.56	universal stress protein PHOS32-like [Cucurbita pepo subsp. pepo]	[more]
XP_022983606.1	7.3e-83	95.12	universal stress protein PHOS32-like [Cucurbita maxima]	[more]
XP_038903504.1	6.0e-77	89.02	universal stress protein PHOS32 [Benincasa hispida]	[more]

BLAST of Carg12512-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VYN9	4.8e-13	32.52	Universal stress protein PHOS32 OS=Arabidopsis thaliana OX=3702 GN=PHOS32 PE=1 S...	[more]
Q8L4N1	2.7e-11	33.53	Universal stress protein PHOS34 OS=Arabidopsis thaliana OX=3702 GN=PHOS34 PE=1 S...	[more]
Q57951	6.1e-08	32.48	Universal stress protein MJ0531 OS=Methanocaldococcus jannaschii (strain ATCC 43...	[more]
P87132	1.8e-07	28.00	Uncharacterized protein C167.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC ...	[more]
Q57997	4.9e-05	37.65	Universal stress protein MJ0577 OS=Methanocaldococcus jannaschii (strain ATCC 43...	[more]

BLAST of Carg12512-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FAU1	7.6e-86	98.78	universal stress protein PHOS32-like OS=Cucurbita moschata OX=3662 GN=LOC1114423...	[more]
A0A6J1J2T3	3.5e-83	95.12	universal stress protein PHOS32-like OS=Cucurbita maxima OX=3661 GN=LOC111482162...	[more]
A0A0A0LDK3	4.9e-77	89.02	Usp domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G733270 PE=4 SV...	[more]
A0A5A7TSD0	1.4e-76	87.80	Universal stress protein PHOS32-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3B5T0	1.4e-76	87.80	uncharacterized protein C167.05 OS=Cucumis melo OX=3656 GN=LOC103486344 PE=4 SV=...	[more]

BLAST of Carg12512-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53990.1	9.9e-62	67.50	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G03270.2	1.5e-46	55.41	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G17020.1	9.0e-39	50.65	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G03270.1	5.5e-36	51.11	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]
AT3G53990.2	2.1e-32	65.63	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006015	Universal stress protein A family	PRINTS	PR01438	UNVRSLSTRESS	coord: 4..22 score: 31.58 coord: 117..129 score: 33.23 coord: 135..157 score: 46.78
IPR006016	UspA	PFAM	PF00582	Usp	coord: 5..157 e-value: 2.6E-34 score: 118.8
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 1..163 e-value: 6.5E-36 score: 126.2
None	No IPR available	PANTHER	PTHR46100	IMP2'P	coord: 4..163
None	No IPR available	PANTHER	PTHR46100:SF2	OS05G0453700 PROTEIN	coord: 4..163
None	No IPR available	CDD	cd00293	USP_Like	coord: 6..156 e-value: 1.46233E-30 score: 105.915
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 5..159

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg12512	Carg12512	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12512-RA:1	Carg12512-RA:1	exon
Carg12512-RA:2	Carg12512-RA:2	exon
Carg12512-RA:3	Carg12512-RA:3	exon
Carg12512-RA:4	Carg12512-RA:4	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12512-RA:five_prime_utr	Carg12512-RA:five_prime_utr	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12512-RA:cds	Carg12512-RA:cds	CDS
Carg12512-RA:cds	Carg12512-RA:cds_2	CDS
Carg12512-RA:cds	Carg12512-RA:cds_3	CDS
Carg12512-RA:cds	Carg12512-RA:cds_4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12512-RA:three_prime_utr	Carg12512-RA:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg12512-RA	Carg12512-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0061077	chaperone-mediated protein folding
biological_process	GO:0009409	response to cold
biological_process	GO:0009408	response to heat
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0034337	RNA folding
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003676	nucleic acid binding