Carg12222-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg12222-RA vs. NCBI nr
Match: KAG6587983.1 (E3 ubiquitin-protein ligase ATL42, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 786.9 bits (2031), Expect = 7.9e-224
Identity = 398/398 (100.00%), Postives = 398/398 (100.00%), Query Frame = 0

Query: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC
Sbjct: 183 MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 242

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120
           HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK
Sbjct: 243 HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 302

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL
Sbjct: 303 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 362

Query: 181 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240
           SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY
Sbjct: 363 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 422

Query: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300
           DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE
Sbjct: 423 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 482

Query: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 360
           STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE
Sbjct: 483 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 542

Query: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 399
           SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Sbjct: 543 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 580

BLAST of Carg12222-RA vs. NCBI nr
Match: KAG7021875.1 (E3 ubiquitin-protein ligase ATL42, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 786.9 bits (2031), Expect = 7.9e-224
Identity = 398/398 (100.00%), Postives = 398/398 (100.00%), Query Frame = 0

Query: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC
Sbjct: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120
           HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK
Sbjct: 61  HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL
Sbjct: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180

Query: 181 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240
           SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY
Sbjct: 181 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240

Query: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300
           DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE
Sbjct: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300

Query: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 360
           STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE
Sbjct: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 360

Query: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 399
           SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Sbjct: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 398

BLAST of Carg12222-RA vs. NCBI nr
Match: XP_022929946.1 (E3 ubiquitin-protein ligase ATL42-like [Cucurbita moschata])

HSP 1 Score: 785.8 bits (2028), Expect = 1.8e-223
Identity = 397/398 (99.75%), Postives = 398/398 (100.00%), Query Frame = 0

Query: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC
Sbjct: 40  MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 99

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120
           HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK
Sbjct: 100 HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 159

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL
Sbjct: 160 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 219

Query: 181 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240
           SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY
Sbjct: 220 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 279

Query: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300
           DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE
Sbjct: 280 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 339

Query: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 360
           STLSRSVENEEPL+GFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE
Sbjct: 340 STLSRSVENEEPLRGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 399

Query: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 399
           SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Sbjct: 400 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 437

BLAST of Carg12222-RA vs. NCBI nr
Match: XP_023531707.1 (E3 ubiquitin-protein ligase ATL42-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 770.4 bits (1988), Expect = 7.6e-219
Identity = 391/398 (98.24%), Postives = 394/398 (98.99%), Query Frame = 0

Query: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MIRLNLVTFC FSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC
Sbjct: 1   MIRLNLVTFCFFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120
           HNRSSVHVNNGVTHGGQIRSSSS+PILGIDKTVIESLPFFRFSTLKGSK+GLECAICLSK
Sbjct: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKRGLECAICLSK 120

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL
Sbjct: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180

Query: 181 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240
           SELKQDSNIELFVQR EEEEEEH QQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY
Sbjct: 181 SELKQDSNIELFVQR-EEEEEEHQQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240

Query: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300
           DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE
Sbjct: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300

Query: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 360
           STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHM FNR+IEMGE
Sbjct: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMNFNRKIEMGE 360

Query: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 399
           SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Sbjct: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 397

BLAST of Carg12222-RA vs. NCBI nr
Match: XP_023006562.1 (E3 ubiquitin-protein ligase ATL42-like [Cucurbita maxima])

HSP 1 Score: 770.0 bits (1987), Expect = 1.0e-218
Identity = 391/398 (98.24%), Postives = 394/398 (98.99%), Query Frame = 0

Query: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC
Sbjct: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120
           HNRSSVHVNNGVTHGGQIRSSSS+PILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK
Sbjct: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL
Sbjct: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180

Query: 181 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240
           SELKQDSNIELFVQREEEEEEEH  QQQQIHGSFRFSIGRSFRKMLK DKEGEMLIQKDY
Sbjct: 181 SELKQDSNIELFVQREEEEEEEH--QQQQIHGSFRFSIGRSFRKMLKGDKEGEMLIQKDY 240

Query: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300
           DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFT LETYLEE
Sbjct: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTFLETYLEE 300

Query: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 360
           STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGV+RFGGDVHMKFNR+IEMGE
Sbjct: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVSRFGGDVHMKFNRKIEMGE 360

Query: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 399
           SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Sbjct: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 396

BLAST of Carg12222-RA vs. ExPASy Swiss-Prot
Match: Q5XF85 (E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 SV=2)

HSP 1 Score: 315.8 bits (808), Expect = 6.7e-85
Identity = 189/397 (47.61%), Postives = 247/397 (62.22%), Query Frame = 0

Query: 31  SFQPNLGFVIGILGVMFVLTFVLLVYAKYCHNRSSVHVNNGVTHGGQIR--------SSS 90
           +F+P+L  V G+L +MF LTFVLLVYAK CH        +   H  ++R        ++S
Sbjct: 32  NFEPSLAVVTGVLAIMFALTFVLLVYAKCCHIDLRSGSGDRRRHDRRLRQGIFFNRSTAS 91

Query: 91  SSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSKFEDIEILRLLPKCKHAFHINCI 150
           S    G+DKT IESLP FRFS LKGSKQGL+C++CLSKFE +EILRLLPKC+HAFHI CI
Sbjct: 92  SDRFSGLDKTAIESLPLFRFSALKGSKQGLDCSVCLSKFESVEILRLLPKCRHAFHIGCI 151

Query: 151 DHWLERHATCPLCRQGVS-SEDLQLFTNSSSMRFLWSNLSELKQDSNIELFVQREEEEEE 210
           D WLE+HATCPLCR  VS  ED  + TN +S RFL  N SE+++DS++EL+++REEEEE 
Sbjct: 152 DQWLEQHATCPLCRDRVSMEEDSSVLTNGNSFRFL--NQSEIREDSSLELYIEREEEEER 211

Query: 211 EHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDYDGDKNMKNLHRHNHKIIISDF 270
            H   ++++ GS RFSIG SFRK+LK   + + L+ +  +     K +H+ NH+I++SD 
Sbjct: 212 IH---REELSGSSRFSIGESFRKILKLGNKEKTLLDEHVNDKDEKKLMHKFNHRIVVSDV 271

Query: 271 VFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLE--------------TYLEESTLSRSV 330
           VF NRWSNVSSSD+MFLN EM++SISS RF+SL+                 EE    R +
Sbjct: 272 VFKNRWSNVSSSDLMFLNSEMVNSISSERFSSLDHVKRGDEEDQIGILRIKEEMEAKRML 331

Query: 331 ENE--EPLKGFPS----TSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGES 390
           EN+       F S    +   S  +     RRSVS+IT V R    +H   +       +
Sbjct: 332 ENKLTSMTTMFSSENGDSGSKSRSVMIEPGRRSVSDITAVPRLSISIHGDCSGS-AAETA 391

Query: 391 SELEN------NVKRQTWYPIARKTVQWFANREKTRQ 393
           S L+N        +R+ W PIARKT QWFANREK  Q
Sbjct: 392 SALQNGGNETEERRRRLWLPIARKTAQWFANREKRSQ 422

BLAST of Carg12222-RA vs. ExPASy Swiss-Prot
Match: Q9SL78 (Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.6e-70
Identity = 172/404 (42.57%), Postives = 241/404 (59.65%), Query Frame = 0

Query: 7   VTFCLFSVLF--YVQAQ---PSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYCH 66
           ++   F ++F  YV AQ   P +  A +  F+P+L  + G+  ++F LTFVLLVYAK  H
Sbjct: 7   ISILFFFIIFLDYVSAQSPPPPNLYATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFH 66

Query: 67  NRSSVHVNNGVTHGGQIRS--------SSSSPILGIDKTVIESLPFFRFSTLKGSKQGLE 126
           N      +   + G +IR         + SS   G+DK  IESLPFFRFS LKG KQGLE
Sbjct: 67  NDLRSETD---SDGERIRHDRLWQGLFNRSSRFSGLDKKAIESLPFFRFSALKGLKQGLE 126

Query: 127 CAICLSKFEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSE-DLQLFTNSSS 186
           C++CLSKFED+EILRLLPKC+HAFHI CID WLE+HATCPLCR  V+ E DL +  NSS+
Sbjct: 127 CSVCLSKFEDVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRNRVNIEDDLSVLGNSST 186

Query: 187 MRFLWSNLSELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEG 246
              + +     ++DS +E++++REE   +          GS RFS   SFRK+LK     
Sbjct: 187 SLRILNQSETREEDSRLEIYIEREEGTND----------GSSRFS---SFRKILKK---- 246

Query: 247 EMLIQKDYDGDKNM---KNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSG 306
            +L+++  +G++N+   K +H+ NH+I++SD VF NRWSN++SSD+ FL  EM++S+SS 
Sbjct: 247 SLLLER--EGNENIDEKKLMHKFNHRIVVSDAVFKNRWSNITSSDLTFLTSEMLNSVSSD 306

Query: 307 RFTSLETYLEESTLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTR---FGG 366
           RF+S++  +    L R  E+ E  +      D S        RR+VSEIT V+R    GG
Sbjct: 307 RFSSVDR-VHRGNL-RDKEDMEMKRMLIKHKDSS--------RRTVSEITTVSREKAVGG 366

Query: 367 DVHMKFNRRIEMGESSELENNVKRQTWYPIARKTVQWFANREKT 391
                     +    +      +R+ W PIAR+T QWF NREK+
Sbjct: 367 SYRGSTASTSQNYAVTATTEERRRRLWLPIARRTAQWFVNREKS 378

BLAST of Carg12222-RA vs. ExPASy Swiss-Prot
Match: Q5EAE9 (RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2)

HSP 1 Score: 152.9 bits (385), Expect = 7.6e-36
Identity = 113/317 (35.65%), Postives = 154/317 (48.58%), Query Frame = 0

Query: 22  PSSQDAENY--SFQPNLGFVIGILGVMFVLTFVLLVYAKYCHNRS-SVHVNN-------- 81
           PS     N+  S  P +  VI +L   F LTF+LL+Y K+C  R+ SV+VN+        
Sbjct: 42  PSPPPRHNFTSSLMPGIAVVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQRFAITR 101

Query: 82  ---GVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSKFEDIEIL 141
              G  +GG +     +   GID++VIESLP FRF  L G K GLECA+CL++FE  E+L
Sbjct: 102 YGGGYYNGGGVVGGRKNS--GIDRSVIESLPVFRFGALSGHKDGLECAVCLARFEPTEVL 161

Query: 142 RLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNLSELKQDS 201
           RLLPKCKHAFH+ C+D WL+ H+TCPLCR  V  ED+ L  + +S    W  L   K +S
Sbjct: 162 RLLPKCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDILLIGDCNS----WFELQFSKDES 221

Query: 202 N--------------IELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSD---- 261
           N              +     R     E   +  +    S   S   SFR+ L S     
Sbjct: 222 NSVNNNPPGLTRFIPVSRISGRHSSAGERASRLNEIRTSSSYKSNPMSFRRSLDSSLKVN 281

Query: 262 --------------------KEGEMLI--QKDYDGDKNMKNLHRHNHKIIISDFVFMN-R 284
                               K+G +LI  ++ ++G        R  H+IIIS     + R
Sbjct: 282 DAGEEKSESVAVNCLDRLQRKDGLLLIPNRESFEG--------RFEHRIIISGGNRDDQR 341

BLAST of Carg12222-RA vs. ExPASy Swiss-Prot
Match: Q8W571 (RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3)

HSP 1 Score: 109.8 bits (273), Expect = 7.3e-23
Identity = 60/148 (40.54%), Postives = 84/148 (56.76%), Query Frame = 0

Query: 22  PSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC----HNRSSVHVNNGVTHGGQ 81
           PS  D +     P+   V  +L  +F LT +L VY ++C     + S+ +  N    G  
Sbjct: 33  PSPPDLQT-GHTPSKTTVFAVLVTLFFLTGLLSVYIRHCARSNPDSSTRYFRNRANDGSS 92

Query: 82  IRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQG---LECAICLSKFEDIEILRLLPKCK 141
            R        G+D  V+ES P F +S++K SK G   LECAICL++ ED E +RLLP C 
Sbjct: 93  RRG-------GLDNAVVESFPVFAYSSVKESKIGSKDLECAICLNELEDHETVRLLPICN 152

Query: 142 HAFHINCIDHWLERHATCPLCRQGVSSE 163
           H FHI+CID WL  HATCP+CR  ++++
Sbjct: 153 HLFHIDCIDTWLYSHATCPVCRSNLTAK 172

BLAST of Carg12222-RA vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 3.6e-22
Identity = 57/151 (37.75%), Postives = 90/151 (59.60%), Query Frame = 0

Query: 16  FYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC--HNRSSVHVNNGVT 75
           F++    SS  +   SF P L  +IGIL    +L     + +KYC  H+++S      + 
Sbjct: 41  FFLDDDSSSSSS---SFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLN 100

Query: 76  HGGQIRSSSSSPIL----GIDKTVIESLPFFRFSTLKGSKQGLECAICLSKFEDIEILRL 135
           H G+   SS+  I     G+++++I+S+  +++ +  G   G +C++CLS+FE+ E LRL
Sbjct: 101 HNGEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRL 160

Query: 136 LPKCKHAFHINCIDHWLERHATCPLCRQGVS 161
           LPKC HAFH+ CID WL+ H+ CPLCR  V+
Sbjct: 161 LPKCNHAFHLPCIDTWLKSHSNCPLCRAFVT 188

BLAST of Carg12222-RA vs. ExPASy TrEMBL
Match: A0A6J1EPJ8 (E3 ubiquitin-protein ligase ATL42-like OS=Cucurbita moschata OX=3662 GN=LOC111436403 PE=4 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 8.5e-224
Identity = 397/398 (99.75%), Postives = 398/398 (100.00%), Query Frame = 0

Query: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC
Sbjct: 40  MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 99

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120
           HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK
Sbjct: 100 HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 159

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL
Sbjct: 160 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 219

Query: 181 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240
           SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY
Sbjct: 220 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 279

Query: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300
           DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE
Sbjct: 280 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 339

Query: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 360
           STLSRSVENEEPL+GFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE
Sbjct: 340 STLSRSVENEEPLRGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 399

Query: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 399
           SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Sbjct: 400 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 437

BLAST of Carg12222-RA vs. ExPASy TrEMBL
Match: A0A6J1KW64 (E3 ubiquitin-protein ligase ATL42-like OS=Cucurbita maxima OX=3661 GN=LOC111499247 PE=4 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 4.8e-219
Identity = 391/398 (98.24%), Postives = 394/398 (98.99%), Query Frame = 0

Query: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC
Sbjct: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120
           HNRSSVHVNNGVTHGGQIRSSSS+PILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK
Sbjct: 61  HNRSSVHVNNGVTHGGQIRSSSSTPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL
Sbjct: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180

Query: 181 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240
           SELKQDSNIELFVQREEEEEEEH  QQQQIHGSFRFSIGRSFRKMLK DKEGEMLIQKDY
Sbjct: 181 SELKQDSNIELFVQREEEEEEEH--QQQQIHGSFRFSIGRSFRKMLKGDKEGEMLIQKDY 240

Query: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300
           DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFT LETYLEE
Sbjct: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTFLETYLEE 300

Query: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGE 360
           STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGV+RFGGDVHMKFNR+IEMGE
Sbjct: 301 STLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVSRFGGDVHMKFNRKIEMGE 360

Query: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 399
           SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV
Sbjct: 361 SSELENNVKRQTWYPIARKTVQWFANREKTRQHRVETV 396

BLAST of Carg12222-RA vs. ExPASy TrEMBL
Match: A0A6J1CUL6 (E3 ubiquitin-protein ligase ATL42-like OS=Momordica charantia OX=3673 GN=LOC111014427 PE=4 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 4.5e-148
Identity = 295/438 (67.35%), Postives = 339/438 (77.40%), Query Frame = 0

Query: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           M RL+LV FCLFSV F+V+AQ   QD+EN +FQP+LGFVIGILGVMF+LTFVLL+YAK+C
Sbjct: 1   MKRLSLVAFCLFSVFFHVEAQIDPQDSENSAFQPSLGFVIGILGVMFLLTFVLLLYAKFC 60

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120
           H RS     + V H  QIRSSS     GIDKTVIESLPFFRFSTLKGSK+GLECA+CLSK
Sbjct: 61  HRRSV--RGDSVNHPAQIRSSSRHS--GIDKTVIESLPFFRFSTLKGSKEGLECAVCLSK 120

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLE+HA+CPLCRQ V SEDL+LFT+SSSMR L SN 
Sbjct: 121 FEDIEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVGSEDLKLFTSSSSMRLLLSNQ 180

Query: 181 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDY 240
           SELKQDSNIELFVQREEE+E+E   Q+QQ HGS RFSIGRSFRK+LKS+K+ EMLIQK  
Sbjct: 181 SELKQDSNIELFVQREEEQEQE---QEQQPHGSSRFSIGRSFRKILKSEKDEEMLIQKAC 240

Query: 241 DGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETYLEE 300
           DGD+NMKNLHR NHKII+SDFVFMNRWSNVSSSD+MFLNKEMI SISS RFTS ET +E+
Sbjct: 241 DGDENMKNLHRLNHKIIVSDFVFMNRWSNVSSSDLMFLNKEMIGSISSSRFTSFETDIEQ 300

Query: 301 STLSRSVENEE---------------------------PLKGFPSTSDC------SARIT 360
           ST  RS++NEE                           P+  FPSTS+       +ARI 
Sbjct: 301 STAPRSMQNEEILKIKEEMEIKRSFDSKFSTISQSNSVPILVFPSTSESAAKPCQTARIN 360

Query: 361 STEARRSVSEITGVTRFGGDVHMKFNRRIEMGESSELENNVK----RQTWYPIARKTVQW 399
           S + RRSVSEITG++RF GD+ +KFN R+E GE S+L NN K    RQ WYPIA++TVQW
Sbjct: 361 SPDERRSVSEITGISRFWGDLDLKFNTRVETGEPSDLGNNGKQERMRQNWYPIAKRTVQW 420

BLAST of Carg12222-RA vs. ExPASy TrEMBL
Match: A0A5D3DZ48 (E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00770 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.4e-146
Identity = 298/444 (67.12%), Postives = 347/444 (78.15%), Query Frame = 0

Query: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MI+L+L    LFSV F V+AQ  SQDAEN +FQP+LGFVIGILGVMF+LTF+LLVYAK+C
Sbjct: 1   MIQLSLNVCFLFSVFFVVEAQIDSQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120
           H R+S+ V +GV H  QIRSSS     GIDKTVIESLPFFRFSTLKG+K+GLECA+CLSK
Sbjct: 61  HRRASISV-DGVNHPRQIRSSSR--FSGIDKTVIESLPFFRFSTLKGTKEGLECAVCLSK 120

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLE+HA+CPLCRQ V SEDL+L +NSSSMRFL SNL
Sbjct: 121 FEDIEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVGSEDLKLLSNSSSMRFLLSNL 180

Query: 181 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQK-- 240
           SELKQDSNIELFVQREEEE+    QQQQ +HGS RFSI RSFRK+LK+DKE EMLI +  
Sbjct: 181 SELKQDSNIELFVQREEEEQ----QQQQILHGSSRFSIRRSFRKILKNDKENEMLIPQAS 240

Query: 241 -DYDGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETY 300
            DY+ D+ MKNLHRHNHKII+SDFVFMNRWSN+SSSD+MFLNKEMID+ISS RFTSL+T 
Sbjct: 241 CDYE-DEKMKNLHRHNHKIIVSDFVFMNRWSNISSSDLMFLNKEMIDAISSCRFTSLDTD 300

Query: 301 LEESTLSRSVENEEPLK-------------------------GFPSTSDCS------ARI 360
           +E+STL RS++ +E LK                         G+PSTS  S       RI
Sbjct: 301 IEQSTLPRSMQIDEILKIKEEMEIKRSFESKLNTKIQSNSILGYPSTSQSSTNPSQTTRI 360

Query: 361 TSTEARRSVSEITGVTRFG--GDVHMKFNRRIEMGESSELENNVK----RQTWYPIARKT 399
           TS +ARRSVSEITG++RFG   D++M FNR++E GESS+LE++VK    R+ WYPIA++T
Sbjct: 361 TSPDARRSVSEITGISRFGHDDDLYMNFNRKVENGESSDLESSVKQERMRKIWYPIAKRT 420

BLAST of Carg12222-RA vs. ExPASy TrEMBL
Match: A0A1S3CHK3 (E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo OX=3656 GN=LOC103500995 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.4e-146
Identity = 298/444 (67.12%), Postives = 347/444 (78.15%), Query Frame = 0

Query: 1   MIRLNLVTFCLFSVLFYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC 60
           MI+L+L    LFSV F V+AQ  SQDAEN +FQP+LGFVIGILGVMF+LTF+LLVYAK+C
Sbjct: 1   MIQLSLNVCFLFSVFFVVEAQIDSQDAENSAFQPSLGFVIGILGVMFLLTFILLVYAKFC 60

Query: 61  HNRSSVHVNNGVTHGGQIRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSK 120
           H R+S+ V +GV H  QIRSSS     GIDKTVIESLPFFRFSTLKG+K+GLECA+CLSK
Sbjct: 61  HRRASISV-DGVNHPRQIRSSSR--FSGIDKTVIESLPFFRFSTLKGTKEGLECAVCLSK 120

Query: 121 FEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNL 180
           FEDIEILRLLPKCKHAFHINCIDHWLE+HA+CPLCRQ V SEDL+L +NSSSMRFL SNL
Sbjct: 121 FEDIEILRLLPKCKHAFHINCIDHWLEKHASCPLCRQRVGSEDLKLLSNSSSMRFLLSNL 180

Query: 181 SELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQK-- 240
           SELKQDSNIELFVQREEEE+    QQQQ +HGS RFSI RSFRK+LK+DKE EMLI +  
Sbjct: 181 SELKQDSNIELFVQREEEEQ----QQQQILHGSSRFSIRRSFRKILKNDKENEMLIPQAS 240

Query: 241 -DYDGDKNMKNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLETY 300
            DY+ D+ MKNLHRHNHKII+SDFVFMNRWSN+SSSD+MFLNKEMID+ISS RFTSL+T 
Sbjct: 241 CDYE-DEKMKNLHRHNHKIIVSDFVFMNRWSNISSSDLMFLNKEMIDAISSCRFTSLDTD 300

Query: 301 LEESTLSRSVENEEPLK-------------------------GFPSTSDCS------ARI 360
           +E+STL RS++ +E LK                         G+PSTS  S       RI
Sbjct: 301 IEQSTLPRSMQIDEILKIKEEMEIKRSFESKLNTKIQSNSILGYPSTSQSSTNPSQTTRI 360

Query: 361 TSTEARRSVSEITGVTRFG--GDVHMKFNRRIEMGESSELENNVK----RQTWYPIARKT 399
           TS +ARRSVSEITG++RFG   D++M FNR++E GESS+LE++VK    R+ WYPIA++T
Sbjct: 361 TSPDARRSVSEITGISRFGHDDDLYMNFNRKVENGESSDLESSVKQERMRKIWYPIAKRT 420

BLAST of Carg12222-RA vs. TAIR 10
Match: AT4G28890.1 (RING/U-box superfamily protein )

HSP 1 Score: 315.8 bits (808), Expect = 4.8e-86
Identity = 189/397 (47.61%), Postives = 247/397 (62.22%), Query Frame = 0

Query: 31  SFQPNLGFVIGILGVMFVLTFVLLVYAKYCHNRSSVHVNNGVTHGGQIR--------SSS 90
           +F+P+L  V G+L +MF LTFVLLVYAK CH        +   H  ++R        ++S
Sbjct: 32  NFEPSLAVVTGVLAIMFALTFVLLVYAKCCHIDLRSGSGDRRRHDRRLRQGIFFNRSTAS 91

Query: 91  SSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSKFEDIEILRLLPKCKHAFHINCI 150
           S    G+DKT IESLP FRFS LKGSKQGL+C++CLSKFE +EILRLLPKC+HAFHI CI
Sbjct: 92  SDRFSGLDKTAIESLPLFRFSALKGSKQGLDCSVCLSKFESVEILRLLPKCRHAFHIGCI 151

Query: 151 DHWLERHATCPLCRQGVS-SEDLQLFTNSSSMRFLWSNLSELKQDSNIELFVQREEEEEE 210
           D WLE+HATCPLCR  VS  ED  + TN +S RFL  N SE+++DS++EL+++REEEEE 
Sbjct: 152 DQWLEQHATCPLCRDRVSMEEDSSVLTNGNSFRFL--NQSEIREDSSLELYIEREEEEER 211

Query: 211 EHHQQQQQIHGSFRFSIGRSFRKMLKSDKEGEMLIQKDYDGDKNMKNLHRHNHKIIISDF 270
            H   ++++ GS RFSIG SFRK+LK   + + L+ +  +     K +H+ NH+I++SD 
Sbjct: 212 IH---REELSGSSRFSIGESFRKILKLGNKEKTLLDEHVNDKDEKKLMHKFNHRIVVSDV 271

Query: 271 VFMNRWSNVSSSDMMFLNKEMIDSISSGRFTSLE--------------TYLEESTLSRSV 330
           VF NRWSNVSSSD+MFLN EM++SISS RF+SL+                 EE    R +
Sbjct: 272 VFKNRWSNVSSSDLMFLNSEMVNSISSERFSSLDHVKRGDEEDQIGILRIKEEMEAKRML 331

Query: 331 ENE--EPLKGFPS----TSDCSARITSTEARRSVSEITGVTRFGGDVHMKFNRRIEMGES 390
           EN+       F S    +   S  +     RRSVS+IT V R    +H   +       +
Sbjct: 332 ENKLTSMTTMFSSENGDSGSKSRSVMIEPGRRSVSDITAVPRLSISIHGDCSGS-AAETA 391

Query: 391 SELEN------NVKRQTWYPIARKTVQWFANREKTRQ 393
           S L+N        +R+ W PIARKT QWFANREK  Q
Sbjct: 392 SALQNGGNETEERRRRLWLPIARKTAQWFANREKRSQ 422

BLAST of Carg12222-RA vs. TAIR 10
Match: AT2G20030.1 (RING/U-box superfamily protein )

HSP 1 Score: 268.1 bits (684), Expect = 1.1e-71
Identity = 172/404 (42.57%), Postives = 241/404 (59.65%), Query Frame = 0

Query: 7   VTFCLFSVLF--YVQAQ---PSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYCH 66
           ++   F ++F  YV AQ   P +  A +  F+P+L  + G+  ++F LTFVLLVYAK  H
Sbjct: 7   ISILFFFIIFLDYVSAQSPPPPNLYATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFH 66

Query: 67  NRSSVHVNNGVTHGGQIRS--------SSSSPILGIDKTVIESLPFFRFSTLKGSKQGLE 126
           N      +   + G +IR         + SS   G+DK  IESLPFFRFS LKG KQGLE
Sbjct: 67  NDLRSETD---SDGERIRHDRLWQGLFNRSSRFSGLDKKAIESLPFFRFSALKGLKQGLE 126

Query: 127 CAICLSKFEDIEILRLLPKCKHAFHINCIDHWLERHATCPLCRQGVSSE-DLQLFTNSSS 186
           C++CLSKFED+EILRLLPKC+HAFHI CID WLE+HATCPLCR  V+ E DL +  NSS+
Sbjct: 127 CSVCLSKFEDVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRNRVNIEDDLSVLGNSST 186

Query: 187 MRFLWSNLSELKQDSNIELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSDKEG 246
              + +     ++DS +E++++REE   +          GS RFS   SFRK+LK     
Sbjct: 187 SLRILNQSETREEDSRLEIYIEREEGTND----------GSSRFS---SFRKILKK---- 246

Query: 247 EMLIQKDYDGDKNM---KNLHRHNHKIIISDFVFMNRWSNVSSSDMMFLNKEMIDSISSG 306
            +L+++  +G++N+   K +H+ NH+I++SD VF NRWSN++SSD+ FL  EM++S+SS 
Sbjct: 247 SLLLER--EGNENIDEKKLMHKFNHRIVVSDAVFKNRWSNITSSDLTFLTSEMLNSVSSD 306

Query: 307 RFTSLETYLEESTLSRSVENEEPLKGFPSTSDCSARITSTEARRSVSEITGVTR---FGG 366
           RF+S++  +    L R  E+ E  +      D S        RR+VSEIT V+R    GG
Sbjct: 307 RFSSVDR-VHRGNL-RDKEDMEMKRMLIKHKDSS--------RRTVSEITTVSREKAVGG 366

Query: 367 DVHMKFNRRIEMGESSELENNVKRQTWYPIARKTVQWFANREKT 391
                     +    +      +R+ W PIAR+T QWF NREK+
Sbjct: 367 SYRGSTASTSQNYAVTATTEERRRRLWLPIARRTAQWFVNREKS 378

BLAST of Carg12222-RA vs. TAIR 10
Match: AT5G05810.1 (RING/U-box superfamily protein )

HSP 1 Score: 149.1 bits (375), Expect = 7.8e-36
Identity = 109/303 (35.97%), Postives = 149/303 (49.17%), Query Frame = 0

Query: 34  PNLGFVIGILGVMFVLTFVLLVYAKYCHNRS-SVHVNN-----------GVTHGGQIRSS 93
           P +  VI +L   F LTF+LL+Y K+C  R+ SV+VN+           G  +GG +   
Sbjct: 2   PGIAVVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQRFAITRYGGGYYNGGGVVGG 61

Query: 94  SSSPILGIDKTVIESLPFFRFSTLKGSKQGLECAICLSKFEDIEILRLLPKCKHAFHINC 153
             +   GID++VIESLP FRF  L G K GLECA+CL++FE  E+LRLLPKCKHAFH+ C
Sbjct: 62  RKNS--GIDRSVIESLPVFRFGALSGHKDGLECAVCLARFEPTEVLRLLPKCKHAFHVEC 121

Query: 154 IDHWLERHATCPLCRQGVSSEDLQLFTNSSSMRFLWSNLSELKQDSN------------- 213
           +D WL+ H+TCPLCR  V  ED+ L  + +S    W  L   K +SN             
Sbjct: 122 VDTWLDAHSTCPLCRYRVDPEDILLIGDCNS----WFELQFSKDESNSVNNNPPGLTRFI 181

Query: 214 -IELFVQREEEEEEEHHQQQQQIHGSFRFSIGRSFRKMLKSD------------------ 273
            +     R     E   +  +    S   S   SFR+ L S                   
Sbjct: 182 PVSRISGRHSSAGERASRLNEIRTSSSYKSNPMSFRRSLDSSLKVNDAGEEKSESVAVNC 241

Query: 274 ------KEGEMLI--QKDYDGDKNMKNLHRHNHKIIISDFVFMN-RWSNVSSSDMMFLNK 284
                 K+G +LI  ++ ++G        R  H+IIIS     + RWS V  SD+++L  
Sbjct: 242 LDRLQRKDGLLLIPNRESFEG--------RFEHRIIISGGNRDDQRWSEVRPSDLLYLRS 290

BLAST of Carg12222-RA vs. TAIR 10
Match: AT4G40070.1 (RING/U-box superfamily protein )

HSP 1 Score: 109.8 bits (273), Expect = 5.2e-24
Identity = 60/148 (40.54%), Postives = 84/148 (56.76%), Query Frame = 0

Query: 22  PSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC----HNRSSVHVNNGVTHGGQ 81
           PS  D +     P+   V  +L  +F LT +L VY ++C     + S+ +  N    G  
Sbjct: 33  PSPPDLQT-GHTPSKTTVFAVLVTLFFLTGLLSVYIRHCARSNPDSSTRYFRNRANDGSS 92

Query: 82  IRSSSSSPILGIDKTVIESLPFFRFSTLKGSKQG---LECAICLSKFEDIEILRLLPKCK 141
            R        G+D  V+ES P F +S++K SK G   LECAICL++ ED E +RLLP C 
Sbjct: 93  RRG-------GLDNAVVESFPVFAYSSVKESKIGSKDLECAICLNELEDHETVRLLPICN 152

Query: 142 HAFHINCIDHWLERHATCPLCRQGVSSE 163
           H FHI+CID WL  HATCP+CR  ++++
Sbjct: 153 HLFHIDCIDTWLYSHATCPVCRSNLTAK 172

BLAST of Carg12222-RA vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 107.5 bits (267), Expect = 2.6e-23
Identity = 57/151 (37.75%), Postives = 90/151 (59.60%), Query Frame = 0

Query: 16  FYVQAQPSSQDAENYSFQPNLGFVIGILGVMFVLTFVLLVYAKYC--HNRSSVHVNNGVT 75
           F++    SS  +   SF P L  +IGIL    +L     + +KYC  H+++S      + 
Sbjct: 41  FFLDDDSSSSSS---SFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLN 100

Query: 76  HGGQIRSSSSSPIL----GIDKTVIESLPFFRFSTLKGSKQGLECAICLSKFEDIEILRL 135
           H G+   SS+  I     G+++++I+S+  +++ +  G   G +C++CLS+FE+ E LRL
Sbjct: 101 HNGEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRL 160

Query: 136 LPKCKHAFHINCIDHWLERHATCPLCRQGVS 161
           LPKC HAFH+ CID WL+ H+ CPLCR  V+
Sbjct: 161 LPKCNHAFHLPCIDTWLKSHSNCPLCRAFVT 188