Carg12144-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12144-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	acyl-protein thioesterase 2-like
Location	Carg_Chr11: 2452090 .. 2453535 (+)
Sequence length	726
RNA-Seq Expression	Carg12144-RA
Synteny	Carg12144-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTAAAACTGCCAGAAAGGCAATTGAGTTTGGTAAAACCCATGTAGTGAGGCCCAAGGGAAGACACCAAGCTACTGTTGTCTGGCTTCATGGTCTTGGTGATAATGGCGCAAGGTTTTCTTCTTTCTTTCTTTCTTTCTGTTCTTTTCTTAATTGTTTTTATTATGTTTAGCAATGTTTTGATTTTGTTCGACAGCTGGTCGCAGCTCTTGGAGACCCTTCCCCTACCAAATGTAAGAATATGAAGCTTATCAAACTATTTATAAGCATTTACACTGCTATTCTCCATGGTGGGAAGATGAATTTAAGCTTGGAGTTATTGATGCAGATTAAATGGATATGTCCAACTGCACCTACTCGACCGATAGCTTTATTTGGTGGCTTCCCTTCCACTGCTTGTAAGCTTTACTGAAATCCCTTTTCCTTTAATTATTCAACGTCGCTCGAACCGGGATCTCCATGCTGCAAAGTCTAGTTGTTATTCATGTATTTTACAATAACAAGAGCATCATGACTGATATCTTATATATTCAAAGACTGATGTTCTTGTTAAACCAGGGTTTGATGTGGAAGATCTTTCAGAAGATGGTCCAGATGATTTAGAGGGGTTGGATGCTTCAGCTGCTCACGTTGCATATTTGTTATCAACAGAGCCATCTGACAGTAAGTTTCTCTTGCCCTTCTAAATACTATTTGTTTTTGCTTTTTCTACTGATTGTTGGCGTTATTCTGCTGTAATCTTTTTTACAGTTAAGCTTGCTGTAGGAGGGTTCAGTATGGGAGCAGCTACTGCGCTTTACTCGGCGACCTGCCATTCACTGGGAAAATATGGGAATGGCAATCCTTATCCAGCCAATTTGAGTGCAGTTGTGGGGCTTAGTGGCTGGCTTCCATGTTCAAAGTTGGCTTTTTAATTTTGTCATCTGTACAATCTTTGATCCTTTTAAAATCAATAGAAAGCTTCATTTAACGAGTTATCGTGGTGATTCCAGGACTTTGAAGAGCAAGATGGAAGAGATGAAAGCTAGTAATAGTGGAGCGGGATCATTGCCTGTTTTGCTATGTCATGGAAAAGGTATTTATGAATTTATAATTGTTCATGCAGAATATTACGTTACAAATTTGAGATTGTTGCATTTCTGATAATGGTTTGGTGTGAAACGTGTTGCAGTTGATGATGTGGTTCTTTATAAATTTGGAGAGAAATCTTCACAGGCATTACGTTCATGTGGATTTCAAGACGTTACGTTTAAATCCTACAACACGTATGTGACTTTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTAAAATCAAGAAACTAAGTAACGTGTTGTTTGATATCGAGTCGTGTTTTGTTTACAGACTTGGGCATTACACAGTTCCTGAAGAGATGGATGAGGTTTGCGCCTGGTTAACTTCAAAGTTGGGGCTATAG

mRNA sequence

GTTAAAACTGCCAGAAAGGCAATTGAGTTTGGTAAAACCCATGTAGTGAGGCCCAAGGGAAGACACCAAGCTACTGTTGTCTGGCTTCATGGTCTTGGTGATAATGGCGCAAGCTGGTCGCAGCTCTTGGAGACCCTTCCCCTACCAAATATTAAATGGATATGTCCAACTGCACCTACTCGACCGATAGCTTTATTTGGTGGCTTCCCTTCCACTGCTTGGTTTGATGTGGAAGATCTTTCAGAAGATGGTCCAGATGATTTAGAGGGGTTGGATGCTTCAGCTGCTCACGTTGCATATTTGTTATCAACAGAGCCATCTGACATTAAGCTTGCTGTAGGAGGGTTCAGTATGGGAGCAGCTACTGCGCTTTACTCGGCGACCTGCCATTCACTGGGAAAATATGGGAATGGCAATCCTTATCCAGCCAATTTGAGTGCAGTTGTGGGGCTTAGTGGCTGGCTTCCATGTTCAAAGACTTTGAAGAGCAAGATGGAAGAGATGAAAGCTAGTAATAGTGGAGCGGGATCATTGCCTGTTTTGCTATGTCATGGAAAAGTTGATGATGTGGTTCTTTATAAATTTGGAGAGAAATCTTCACAGGCATTACGTTCATGTGGATTTCAAGACGTTACGTTTAAATCCTACAACACACTTGGGCATTACACAGTTCCTGAAGAGATGGATGAGGTTTGCGCCTGGTTAACTTCAAAGTTGGGGCTATAG

Coding sequence (CDS)

GTTAAAACTGCCAGAAAGGCAATTGAGTTTGGTAAAACCCATGTAGTGAGGCCCAAGGGAAGACACCAAGCTACTGTTGTCTGGCTTCATGGTCTTGGTGATAATGGCGCAAGCTGGTCGCAGCTCTTGGAGACCCTTCCCCTACCAAATATTAAATGGATATGTCCAACTGCACCTACTCGACCGATAGCTTTATTTGGTGGCTTCCCTTCCACTGCTTGGTTTGATGTGGAAGATCTTTCAGAAGATGGTCCAGATGATTTAGAGGGGTTGGATGCTTCAGCTGCTCACGTTGCATATTTGTTATCAACAGAGCCATCTGACATTAAGCTTGCTGTAGGAGGGTTCAGTATGGGAGCAGCTACTGCGCTTTACTCGGCGACCTGCCATTCACTGGGAAAATATGGGAATGGCAATCCTTATCCAGCCAATTTGAGTGCAGTTGTGGGGCTTAGTGGCTGGCTTCCATGTTCAAAGACTTTGAAGAGCAAGATGGAAGAGATGAAAGCTAGTAATAGTGGAGCGGGATCATTGCCTGTTTTGCTATGTCATGGAAAAGTTGATGATGTGGTTCTTTATAAATTTGGAGAGAAATCTTCACAGGCATTACGTTCATGTGGATTTCAAGACGTTACGTTTAAATCCTACAACACACTTGGGCATTACACAGTTCCTGAAGAGATGGATGAGGTTTGCGCCTGGTTAACTTCAAAGTTGGGGCTATAG

Protein sequence

VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL

Homology

[Close]

BLAST of Carg12144-RA vs. NCBI nr
Match: KAG7021798.1 (Acyl-protein thioesterase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 505.0 bits (1299), Expect = 3.6e-139
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0


Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV
Sbjct: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240

Query: 241 L 242
           L
Sbjct: 241 L 241

[Close]

BLAST of Carg12144-RA vs. NCBI nr
Match: XP_022925495.1 (acyl-protein thioesterase 2-like [Cucurbita moschata] >XP_022925502.1 acyl-protein thioesterase 2-like [Cucurbita moschata] >XP_023530534.1 acyl-protein thioesterase 2-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023530535.1 acyl-protein thioesterase 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.0 bits (1299), Expect = 3.6e-139
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0


Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 12  VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 71

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 72  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 131

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV
Sbjct: 132 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 191

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 192 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 251

Query: 241 L 242
           L
Sbjct: 252 L 252

[Close]

BLAST of Carg12144-RA vs. NCBI nr
Match: KAG6587912.1 (Acyl-protein thioesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 505.0 bits (1299), Expect = 3.6e-139
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0


Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 118 VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 177

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 178 RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 237

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV
Sbjct: 238 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 297

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 298 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 357

Query: 241 L 242
           L
Sbjct: 358 L 358

[Close]

BLAST of Carg12144-RA vs. NCBI nr
Match: XP_023530533.1 (acyl-protein thioesterase 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.0 bits (1299), Expect = 3.6e-139
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0


Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 36  VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 95

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 96  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 155

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV
Sbjct: 156 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 215

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 216 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 275

Query: 241 L 242
           L
Sbjct: 276 L 276

[Close]

BLAST of Carg12144-RA vs. NCBI nr
Match: XP_023004193.1 (acyl-protein thioesterase 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 502.3 bits (1292), Expect = 2.3e-138
Identity = 240/241 (99.59%), Postives = 240/241 (99.59%), Query Frame = 0


Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 36  VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 95

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 96  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 155

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKM EMKASNSGAGSLPV
Sbjct: 156 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMVEMKASNSGAGSLPV 215

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 216 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 275

Query: 241 L 242
           L
Sbjct: 276 L 276

[Close]

BLAST of Carg12144-RA vs. ExPASy Swiss-Prot
Match: O95372 (Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 5.2e-32
Identity = 82/225 (36.44%), Postives = 123/225 (54.67%), Query Frame = 0


Query: 21  RHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDL 80
           R  A V++LHGLGD G SW+  L T+ LP++K+ICP AP  P+ L       +WFD+  L
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 81  SEDGPDDLEGLDASAAHVAYLLSTEPSD----IKLAVGGFSMGAATALYSA-TCHSLGKY 140
           S D P+D  G+  +A ++  L+  E  +     ++ +GGFS G A +LY+A TC      
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPANRIVLGGFSQGGALSLYTALTC------ 141

Query: 141 GNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHGKVDDVVLYKF 200
               P+P  L+ +V LS WLP  +         +A+N  A  L +L CHG++D +V  +F
Sbjct: 142 ----PHP--LAGIVALSCWLPLHRAFP------QAANGSAKDLAILQCHGELDPMVPVRF 201

Query: 201 GEKSSQALRSCGF-QDVTFKSYNTLGHYTVPEEMDEVCAWLTSKL 240
           G  +++ LRS      V FK+Y  + H + P+EM  V  +L   L
Sbjct: 202 GALTAEKLRSVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

[Close]

BLAST of Carg12144-RA vs. ExPASy Swiss-Prot
Match: Q9WTL7 (Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.2e-31
Identity = 81/225 (36.00%), Postives = 123/225 (54.67%), Query Frame = 0


Query: 21  RHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDL 80
           R  A V++LHGLGD G SW+  L T+ LP++K+ICP AP  P+ L       +WFD+  L
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 81  SEDGPDDLEGLDASAAHVAYLLSTEPSD----IKLAVGGFSMGAATALYSA-TCHSLGKY 140
           S D P+D  G+  +A ++  L+  E  +     ++ +GGFS G A +LY+A TC      
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPANRIVLGGFSQGGALSLYTALTC------ 141

Query: 141 GNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHGKVDDVVLYKF 200
               P+P  L+ +V LS WLP  +         +A+N  A  L +L CHG++D +V  +F
Sbjct: 142 ----PHP--LAGIVALSCWLPLHRNFP------QAANGSAKDLAILQCHGELDPMVPVRF 201

Query: 201 GEKSSQALRSCGF-QDVTFKSYNTLGHYTVPEEMDEVCAWLTSKL 240
           G  +++ LR+      V FK+Y  + H + P+EM  V  +L   L
Sbjct: 202 GALTAEKLRTVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

[Close]

BLAST of Carg12144-RA vs. ExPASy Swiss-Prot
Match: Q9QYL8 (Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.2e-31
Identity = 81/225 (36.00%), Postives = 123/225 (54.67%), Query Frame = 0


Query: 21  RHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTRPIALFGGFPSTAWFDVEDL 80
           R  A V++LHGLGD G SW+  L T+ LP++K+ICP AP  P+ L       +WFD+  L
Sbjct: 22  RETAAVIFLHGLGDTGHSWADALSTIRLPHVKYICPHAPRIPVTLNMKMVMPSWFDLMGL 81

Query: 81  SEDGPDDLEGLDASAAHVAYLLSTEPSD----IKLAVGGFSMGAATALYSA-TCHSLGKY 140
           S D P+D  G+  +A ++  L+  E  +     ++ +GGFS G A +LY+A TC      
Sbjct: 82  SPDAPEDEAGIKKAAENIKALIEHEMKNGIPANRIVLGGFSQGGALSLYTALTC------ 141

Query: 141 GNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHGKVDDVVLYKF 200
               P+P  L+ +V LS WLP  +         +A+N  A  L +L CHG++D +V  +F
Sbjct: 142 ----PHP--LAGIVALSCWLPLHRNFP------QAANGSAKDLAILQCHGELDPMVPVRF 201

Query: 201 GEKSSQALRSCGF-QDVTFKSYNTLGHYTVPEEMDEVCAWLTSKL 240
           G  +++ LR+      V FK+Y  + H + P+EM  V  +L   L
Sbjct: 202 GALTAEKLRTVVTPARVQFKTYPGVMHSSCPQEMAAVKEFLEKLL 228

[Close]

BLAST of Carg12144-RA vs. ExPASy Swiss-Prot
Match: Q4I8Q4 (Acyl-protein thioesterase 1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=FGRAMPH1_01T20223 PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 2.3e-27
Identity = 79/233 (33.91%), Postives = 122/233 (52.36%), Query Frame = 0


Query: 21  RHQATVVWLHGLGDNGASWSQLLET----LPLPNIKWICPTAPTRPIALFGGFPSTAWFD 80
           +H ATV+++HGLGD G  W+  +E       +  +K+I P AP  PI++  G     WFD
Sbjct: 16  QHTATVIFVHGLGDTGHGWASAVENWRRRQKMDEVKFILPHAPQIPISVNMGMRMPGWFD 75

Query: 81  VEDLSED-----GPDDLEGLDASAAHVAYLLSTE-PSDI---KLAVGGFSMGAATALYSA 140
           ++ L  D       +D EG+  S  +   L+  E  S I   ++ +GGFS G A +L + 
Sbjct: 76  IKQLGGDVDSLIRNEDTEGIKLSQKYFHNLIQQEIDSGIVPERIVLGGFSQGGAMSLLAG 135

Query: 141 -TCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHGK 200
            TC S             L  ++GLS WL  SKT    ++   A+       PV++ HG+
Sbjct: 136 LTCTS------------KLGGILGLSSWLLLSKTFADMVKPTDANR----QTPVMMFHGE 195

Query: 201 VDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKL 240
            D +V  + G+ S++ L+  G+ DV +K+Y  +GH  VPEE+DEV A+L  +L
Sbjct: 196 EDPIVPCERGKLSAELLKGLGY-DVAWKTYPGMGHSAVPEELDEVEAFLRKQL 231

[Close]

BLAST of Carg12144-RA vs. ExPASy Swiss-Prot
Match: P0CL95 (Acyl-protein thioesterase 1 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=CNBF2260 PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.9e-27
Identity = 77/245 (31.43%), Postives = 120/245 (48.98%), Query Frame = 0


Query: 16  VRPKGRHQATVVWLHGLGDNGASWSQLLETL--PLPNIKWICPTAPTRPIALFGGFPSTA 75
           + PK  H ATV++LHGLGD+G  W  + + L    PN+KWI P APT P++L  G    +
Sbjct: 10  ISPKEAHTATVIFLHGLGDSGHGWLPVAKMLWSSFPNVKWILPHAPTIPVSLNHGMAMPS 69

Query: 76  WFDV---EDLSEDGPDDLEGLDASAAHVAYLLSTEPS----DIKLAVGGFSMGAATALYS 135
           WFD+   + L     DD +G+  +   V  L+  E      + ++ +GGFS G A ++  
Sbjct: 70  WFDIRHLDKLDNSENDDEQGMLETLKSVDELIQAEVDSGIPENRIVLGGFSQGGAISVL- 129

Query: 136 ATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHGK 195
                     N       L+ VV LS W+P +  +   M E       A  +PV   HG 
Sbjct: 130 ----------NMLTTKRKLAGVVALSTWVPLNHKIVQMMSE------HAKDIPVFWGHGT 189

Query: 196 VDDVVLYKFGEKSSQAL-RSCGFQ-----------DVTFKSYNTLGHYTVPEEMDEVCAW 240
            D VV Y+FG++S   L + CG++            + F+SY  + H + P+E++++ +W
Sbjct: 190 NDPVVDYRFGQRSVDFLVQKCGYKLLSQGTTFARPGIRFESYPGMPHSSCPQEIEDLKSW 237

[Close]

BLAST of Carg12144-RA vs. ExPASy TrEMBL
Match: A0A6J1EBV8 (acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432778 PE=4 SV=1)

HSP 1 Score: 505.0 bits (1299), Expect = 1.8e-139
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0


Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 12  VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 71

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 72  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 131

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV
Sbjct: 132 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 191

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 192 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 251

Query: 241 L 242
           L
Sbjct: 252 L 252

[Close]

BLAST of Carg12144-RA vs. ExPASy TrEMBL
Match: A0A6J1KTX4 (acyl-protein thioesterase 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111497590 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.1e-138
Identity = 240/241 (99.59%), Postives = 240/241 (99.59%), Query Frame = 0


Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 12  VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 71

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 72  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 131

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKM EMKASNSGAGSLPV
Sbjct: 132 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMVEMKASNSGAGSLPV 191

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 192 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 251

Query: 241 L 242
           L
Sbjct: 252 L 252

[Close]

BLAST of Carg12144-RA vs. ExPASy TrEMBL
Match: A0A6J1KYR2 (acyl-protein thioesterase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111497590 PE=4 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 1.1e-138
Identity = 240/241 (99.59%), Postives = 240/241 (99.59%), Query Frame = 0


Query: 1   VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 60
           VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT
Sbjct: 36  VKTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPT 95

Query: 61  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 120
           RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA
Sbjct: 96  RPIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGA 155

Query: 121 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPV 180
           ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKM EMKASNSGAGSLPV
Sbjct: 156 ATALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMVEMKASNSGAGSLPV 215

Query: 181 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 240
           LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG
Sbjct: 216 LLCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLG 275

Query: 241 L 242
           L
Sbjct: 276 L 276

[Close]

BLAST of Carg12144-RA vs. ExPASy TrEMBL
Match: A0A0A0LU24 (Abhydrolase_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G305760 PE=4 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 2.8e-129
Identity = 219/240 (91.25%), Postives = 233/240 (97.08%), Query Frame = 0


Query: 2   KTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTR 61
           + A+KAIEFGKT+VVRPKG+HQATVVWLHGLGDNG+SWSQLLETLPLPNIKWICPTAPTR
Sbjct: 13  RAAKKAIEFGKTYVVRPKGKHQATVVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPTR 72

Query: 62  PIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAA 121
           PIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEP+DIKL VGGFSMGAA
Sbjct: 73  PIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPADIKLGVGGFSMGAA 132

Query: 122 TALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVL 181
           TALYSATCH++GKYGNGNPYPANLSAVVGLSGWLPCSKTLK+ ME+  A NSGAGSLP+L
Sbjct: 133 TALYSATCHAVGKYGNGNPYPANLSAVVGLSGWLPCSKTLKTNMEQKNAGNSGAGSLPIL 192

Query: 182 LCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL 241
           LCHGKVDDVVLYKFGEKSS+ALRS GF+DVTFKSYN+LGHYTVPEEMDEVCAWLTSKLGL
Sbjct: 193 LCHGKVDDVVLYKFGEKSSEALRSSGFKDVTFKSYNSLGHYTVPEEMDEVCAWLTSKLGL 252

[Close]

BLAST of Carg12144-RA vs. ExPASy TrEMBL
Match: A0A6J1JJZ2 (acyl-protein thioesterase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485265 PE=4 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 3.7e-129
Identity = 221/240 (92.08%), Postives = 230/240 (95.83%), Query Frame = 0


Query: 2   KTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTR 61
           K AR+AIEFGKT+VVRPKG+HQAT+VWLHGLGDNG+SWSQLLE LPLPNIKWICPTAPTR
Sbjct: 13  KAARRAIEFGKTYVVRPKGKHQATIVWLHGLGDNGSSWSQLLEMLPLPNIKWICPTAPTR 72

Query: 62  PIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAA 121
           PIALFGGFPSTAWFDV+DLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKL VGGFSMGAA
Sbjct: 73  PIALFGGFPSTAWFDVKDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLGVGGFSMGAA 132

Query: 122 TALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVL 181
           TALYSATCH++GKYGNGNPYP NLSAVVGLSGWLPCSKTLKSKMEE  ASNSGAGSLP+L
Sbjct: 133 TALYSATCHAVGKYGNGNPYPTNLSAVVGLSGWLPCSKTLKSKMEEKNASNSGAGSLPIL 192

Query: 182 LCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL 241
           LCHGKVDDVVLYKFGEKSSQAL S GFQDV FKSYN LGHYTVPEEMDEVCAWLTSKLGL
Sbjct: 193 LCHGKVDDVVLYKFGEKSSQALHSVGFQDVIFKSYNGLGHYTVPEEMDEVCAWLTSKLGL 252

[Close]

BLAST of Carg12144-RA vs. TAIR 10
Match: AT5G20060.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 403.7 bits (1036), Expect = 1.1e-112
Identity = 182/240 (75.83%), Postives = 214/240 (89.17%), Query Frame = 0


Query: 2   KTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTR 61
           +  R+A+EFGKTHVVRPKG+HQAT+VWLHGLGDNG+SWSQLLETLPLPNIKWICPTAP++
Sbjct: 12  RNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPSQ 71

Query: 62  PIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAA 121
           PI+LFGGFPSTAWFDV D++EDGPDD+EGLD +AAHVA LLS EP+DIKL VGGFSMGAA
Sbjct: 72  PISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAHVANLLSNEPADIKLGVGGFSMGAA 131

Query: 122 TALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVL 181
           T+LYSATC +LGKYGNGNPYP NLSA++GLSGWLPC+KTL  K+EE +  N  A SLP++
Sbjct: 132 TSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPCAKTLAGKLEEEQIKNR-AASLPIV 191

Query: 182 LCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL 241
           +CHGK DDVV +KFGEKSSQAL S GF+ VTFK Y+ LGH+T+P+E+DE+CAWLTS L L
Sbjct: 192 VCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELDELCAWLTSTLSL 250

[Close]

BLAST of Carg12144-RA vs. TAIR 10
Match: AT5G20060.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 403.7 bits (1036), Expect = 1.1e-112
Identity = 182/240 (75.83%), Postives = 214/240 (89.17%), Query Frame = 0


Query: 2   KTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTR 61
           +  R+A+EFGKTHVVRPKG+HQAT+VWLHGLGDNG+SWSQLLETLPLPNIKWICPTAP++
Sbjct: 12  RNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPSQ 71

Query: 62  PIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAA 121
           PI+LFGGFPSTAWFDV D++EDGPDD+EGLD +AAHVA LLS EP+DIKL VGGFSMGAA
Sbjct: 72  PISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAHVANLLSNEPADIKLGVGGFSMGAA 131

Query: 122 TALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVL 181
           T+LYSATC +LGKYGNGNPYP NLSA++GLSGWLPC+KTL  K+EE +  N  A SLP++
Sbjct: 132 TSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPCAKTLAGKLEEEQIKNR-AASLPIV 191

Query: 182 LCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL 241
           +CHGK DDVV +KFGEKSSQAL S GF+ VTFK Y+ LGH+T+P+E+DE+CAWLTS L L
Sbjct: 192 VCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELDELCAWLTSTLSL 250

[Close]

BLAST of Carg12144-RA vs. TAIR 10
Match: AT5G20060.3 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 403.7 bits (1036), Expect = 1.1e-112
Identity = 182/240 (75.83%), Postives = 214/240 (89.17%), Query Frame = 0


Query: 2   KTARKAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTR 61
           +  R+A+EFGKTHVVRPKG+HQAT+VWLHGLGDNG+SWSQLLETLPLPNIKWICPTAP++
Sbjct: 12  RNLRRAVEFGKTHVVRPKGKHQATIVWLHGLGDNGSSWSQLLETLPLPNIKWICPTAPSQ 71

Query: 62  PIALFGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAA 121
           PI+LFGGFPSTAWFDV D++EDGPDD+EGLD +AAHVA LLS EP+DIKL VGGFSMGAA
Sbjct: 72  PISLFGGFPSTAWFDVVDINEDGPDDMEGLDVAAAHVANLLSNEPADIKLGVGGFSMGAA 131

Query: 122 TALYSATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVL 181
           T+LYSATC +LGKYGNGNPYP NLSA++GLSGWLPC+KTL  K+EE +  N  A SLP++
Sbjct: 132 TSLYSATCFALGKYGNGNPYPINLSAIIGLSGWLPCAKTLAGKLEEEQIKNR-AASLPIV 191

Query: 182 LCHGKVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL 241
           +CHGK DDVV +KFGEKSSQAL S GF+ VTFK Y+ LGH+T+P+E+DE+CAWLTS L L
Sbjct: 192 VCHGKADDVVPFKFGEKSSQALLSNGFKKVTFKPYSALGHHTIPQELDELCAWLTSTLSL 250

[Close]

BLAST of Carg12144-RA vs. TAIR 10
Match: AT3G15650.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 339.7 bits (870), Expect = 1.9e-93
Identity = 155/236 (65.68%), Postives = 189/236 (80.08%), Query Frame = 0


Query: 6   KAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTRPIAL 65
           +  EFG+T+VVRPKG+HQAT+VWLHGLGDNG+S SQLLE+LPLPNIKWICPTAP+RP++L
Sbjct: 16  RGYEFGRTYVVRPKGKHQATIVWLHGLGDNGSSSSQLLESLPLPNIKWICPTAPSRPVSL 75

Query: 66  FGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAATALY 125
            GGFP TAWFDV ++SED  DD+EGLDASAAH+A LLS EP+D+K+ +GGFSMGAA ALY
Sbjct: 76  LGGFPCTAWFDVGEISEDLHDDIEGLDASAAHIANLLSAEPTDVKVGIGGFSMGAAIALY 135

Query: 126 SATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHG 185
           S TC++LG+YG G+ Y  NL A VGLSGWLP  ++L+SK+E        A S+P+LL HG
Sbjct: 136 STTCYALGRYGTGHAYTINLRATVGLSGWLPGWRSLRSKIESSNEVARRAASIPILLAHG 195

Query: 186 KVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL 242
             DDVV Y+FGEKS+ +L   GF+   FK Y  LGHYTVP+EMDEV  WL S+LGL
Sbjct: 196 TSDDVVPYRFGEKSAHSLAMAGFRQTMFKPYEGLGHYTVPKEMDEVVHWLVSRLGL 251

[Close]

BLAST of Carg12144-RA vs. TAIR 10
Match: AT1G52700.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 335.5 bits (859), Expect = 3.5e-92
Identity = 155/236 (65.68%), Postives = 191/236 (80.93%), Query Frame = 0


Query: 6   KAIEFGKTHVVRPKGRHQATVVWLHGLGDNGASWSQLLETLPLPNIKWICPTAPTRPIAL 65
           +  EFG+T+VVRPKG+HQAT+VWLHGLGDNG+S SQL+++L LPNIKWICPTAP+RP+  
Sbjct: 16  RGYEFGRTYVVRPKGKHQATLVWLHGLGDNGSSSSQLMDSLHLPNIKWICPTAPSRPVTS 75

Query: 66  FGGFPSTAWFDVEDLSEDGPDDLEGLDASAAHVAYLLSTEPSDIKLAVGGFSMGAATALY 125
            GGF  TAWFDV ++SEDG DDLEGLDASA+H+A LLS+EP+D+K+ +GGFSMGAA +LY
Sbjct: 76  LGGFTCTAWFDVGEISEDGHDDLEGLDASASHIANLLSSEPADVKVGIGGFSMGAAISLY 135

Query: 126 SATCHSLGKYGNGNPYPANLSAVVGLSGWLPCSKTLKSKMEEMKASNSGAGSLPVLLCHG 185
           SATC++LG+YG G+ YP NL AVVGLSGWLP  K+L+SK+E    +   A SLP++L HG
Sbjct: 136 SATCYALGRYGTGHAYPINLQAVVGLSGWLPGWKSLRSKIECSFEAARRAASLPIILTHG 195

Query: 186 KVDDVVLYKFGEKSSQALRSCGFQDVTFKSYNTLGHYTVPEEMDEVCAWLTSKLGL 242
             DDVV Y+FGEKS+Q+L   GF+   FK Y  LGHYTVP EMDEV  WLT+ LGL
Sbjct: 196 TSDDVVPYRFGEKSAQSLGMAGFRLAMFKPYEGLGHYTVPREMDEVVHWLTTMLGL 251

The following BLAST results are available for this feature:

BLAST of Carg12144-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021798.1	3.6e-139	100.00	Acyl-protein thioesterase 2, partial [Cucurbita argyrosperma subsp. argyrosperma...	[more]
XP_022925495.1	3.6e-139	100.00	acyl-protein thioesterase 2-like [Cucurbita moschata] >XP_022925502.1 acyl-prote...	[more]
KAG6587912.1	3.6e-139	100.00	Acyl-protein thioesterase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023530533.1	3.6e-139	100.00	acyl-protein thioesterase 2-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_023004193.1	2.3e-138	99.59	acyl-protein thioesterase 2-like isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg12144-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O95372	5.2e-32	36.44	Acyl-protein thioesterase 2 OS=Homo sapiens OX=9606 GN=LYPLA2 PE=1 SV=1	[more]
Q9WTL7	1.2e-31	36.00	Acyl-protein thioesterase 2 OS=Mus musculus OX=10090 GN=Lypla2 PE=1 SV=1	[more]
Q9QYL8	1.2e-31	36.00	Acyl-protein thioesterase 2 OS=Rattus norvegicus OX=10116 GN=Lypla2 PE=1 SV=1	[more]
Q4I8Q4	2.3e-27	33.91	Acyl-protein thioesterase 1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 12365...	[more]
P0CL95	3.9e-27	31.43	Acyl-protein thioesterase 1 OS=Cryptococcus neoformans var. neoformans serotype ...	[more]

BLAST of Carg12144-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EBV8	1.8e-139	100.00	acyl-protein thioesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432778 P...	[more]
A0A6J1KTX4	1.1e-138	99.59	acyl-protein thioesterase 2-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1KYR2	1.1e-138	99.59	acyl-protein thioesterase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A0A0LU24	2.8e-129	91.25	Abhydrolase_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G3057...	[more]
A0A6J1JJZ2	3.7e-129	92.08	acyl-protein thioesterase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111485265 PE=...	[more]

BLAST of Carg12144-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G20060.2	1.1e-112	75.83	alpha/beta-Hydrolases superfamily protein	[more]
AT5G20060.1	1.1e-112	75.83	alpha/beta-Hydrolases superfamily protein	[more]
AT5G20060.3	1.1e-112	75.83	alpha/beta-Hydrolases superfamily protein	[more]
AT3G15650.1	1.9e-93	65.68	alpha/beta-Hydrolases superfamily protein	[more]
AT1G52700.1	3.5e-92	65.68	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003140	Phospholipase/carboxylesterase/thioesterase	PFAM	PF02230	Abhydrolase_2	coord: 13..235 e-value: 5.5E-40 score: 137.5
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 3..240 e-value: 3.1E-63 score: 215.4
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 14..239
None	No IPR available	PANTHER	PTHR46234:SF9	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 2..241
None	No IPR available	PANTHER	PTHR46234	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 2..241

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg12144	Carg12144	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12144-RA:1	Carg12144-RA:1	exon
Carg12144-RA:2	Carg12144-RA:2	exon
Carg12144-RA:3	Carg12144-RA:3	exon
Carg12144-RA:4	Carg12144-RA:4	exon
Carg12144-RA:5	Carg12144-RA:5	exon
Carg12144-RA:6	Carg12144-RA:6	exon
Carg12144-RA:7	Carg12144-RA:7	exon
Carg12144-RA:8	Carg12144-RA:8	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12144-RA:cds	Carg12144-RA:cds	CDS
Carg12144-RA:cds	Carg12144-RA:cds_2	CDS
Carg12144-RA:cds	Carg12144-RA:cds_3	CDS
Carg12144-RA:cds	Carg12144-RA:cds_4	CDS
Carg12144-RA:cds	Carg12144-RA:cds_5	CDS
Carg12144-RA:cds	Carg12144-RA:cds_6	CDS
Carg12144-RA:cds	Carg12144-RA:cds_7	CDS
Carg12144-RA:cds	Carg12144-RA:cds_8	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg12144-RA	Carg12144-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity