Carg12130-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12130-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ethylene-responsive transcription factor WIN1-like
Location	Carg_Chr11: 2374062 .. 2374800 (+)
Sequence length	660
RNA-Seq Expression	Carg12130-RA
Synteny	Carg12130-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTCCCCCACTGCCCCTTCTCTCTGTACTTCCTTCTCTGACAATGCCCAAGTTTAAGAAGTTTCGTGGTGTTAGGCAGCGCCATTGGGGCTCTTGGGTTTCTGAAATTCGCCATCCATTACTGTAATTGATTCTTCTAATTTCTTCCCCAAATTTTAAATATGTGTATTGTTTAATCAATTTGTGGTTTGATTGCGTGCAGGAAGAGGCGGATTTGGTTGGGGACGTTTGAGACGGCGGAGGAAGCGGCAAGAGCGTATGATCAAGCTGCGATTTTGATGAGCGGACGTAATGCCAAAACCAATTTCCCGACGACCCATGACACCAACATTACTACAACCAAGGATTCCCCCAAGGGGTTGTCTGAAATATTGCAAGCTAAGCTCAAGAAATGCTGTCGGACTCCATCGCCTTCCATGACATGTTTGAGGCTCGACACTGAGAATTCCAACATTGGCGTGTGGCAGAAACGTGCTGGGCCACGGTCGGCATCTAGCTGGGTCATGACTGTTGAGCTTGGAAATAAGAAGAGCCATAACAACGAGGATGATTCTCTGATGGGTGTGGTTGTGGCTGATCAGTCCCCGGTGGCGGCGGAAGTGGCGGAGATTCCATCGGAGTTGGATGAAGAAGAGAGAATGGTGGCTTTGCAAATGGTGGAGGAACTGCTTCATATCAGCTATGATGAACCAAACGAATTGTTTGAGATTCAACAGGGGAAGGATGTTTATCTTTAG

mRNA sequence

ATGTTTCCCCCACTGCCCCTTCTCTCTGTACTTCCTTCTCTGACAATGCCCAAGTTTAAGAAGTTTCGTGGTGTTAGGCAGCGCCATTGGGGCTCTTGGGTTTCTGAAATTCGCCATCCATTACTGAAGAGGCGGATTTGGTTGGGGACGTTTGAGACGGCGGAGGAAGCGGCAAGAGCGTATGATCAAGCTGCGATTTTGATGAGCGGACGTAATGCCAAAACCAATTTCCCGACGACCCATGACACCAACATTACTACAACCAAGGATTCCCCCAAGGGGTTGTCTGAAATATTGCAAGCTAAGCTCAAGAAATGCTGTCGGACTCCATCGCCTTCCATGACATGTTTGAGGCTCGACACTGAGAATTCCAACATTGGCGTGTGGCAGAAACGTGCTGGGCCACGGTCGGCATCTAGCTGGGTCATGACTGTTGAGCTTGGAAATAAGAAGAGCCATAACAACGAGGATGATTCTCTGATGGGTGTGGTTGTGGCTGATCAGTCCCCGGTGGCGGCGGAAGTGGCGGAGATTCCATCGGAGTTGGATGAAGAAGAGAGAATGGTGGCTTTGCAAATGGTGGAGGAACTGCTTCATATCAGCTATGATGAACCAAACGAATTGTTTGAGATTCAACAGGGGAAGGATGTTTATCTTTAG

Coding sequence (CDS)

ATGTTTCCCCCACTGCCCCTTCTCTCTGTACTTCCTTCTCTGACAATGCCCAAGTTTAAGAAGTTTCGTGGTGTTAGGCAGCGCCATTGGGGCTCTTGGGTTTCTGAAATTCGCCATCCATTACTGAAGAGGCGGATTTGGTTGGGGACGTTTGAGACGGCGGAGGAAGCGGCAAGAGCGTATGATCAAGCTGCGATTTTGATGAGCGGACGTAATGCCAAAACCAATTTCCCGACGACCCATGACACCAACATTACTACAACCAAGGATTCCCCCAAGGGGTTGTCTGAAATATTGCAAGCTAAGCTCAAGAAATGCTGTCGGACTCCATCGCCTTCCATGACATGTTTGAGGCTCGACACTGAGAATTCCAACATTGGCGTGTGGCAGAAACGTGCTGGGCCACGGTCGGCATCTAGCTGGGTCATGACTGTTGAGCTTGGAAATAAGAAGAGCCATAACAACGAGGATGATTCTCTGATGGGTGTGGTTGTGGCTGATCAGTCCCCGGTGGCGGCGGAAGTGGCGGAGATTCCATCGGAGTTGGATGAAGAAGAGAGAATGGTGGCTTTGCAAATGGTGGAGGAACTGCTTCATATCAGCTATGATGAACCAAACGAATTGTTTGAGATTCAACAGGGGAAGGATGTTTATCTTTAG

Protein sequence

MFPPLPLLSVLPSLTMPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKTNFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGPRSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVEELLHISYDEPNELFEIQQGKDVYL

Homology

[Close]

BLAST of Carg12130-RA vs. NCBI nr
Match: KAG7021785.1 (Ethylene-responsive transcription factor WIN1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 437.6 bits (1124), Expect = 6.5e-119
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 0


Query: 1   MFPPLPLLSVLPSLTMPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARA 60
           MFPPLPLLSVLPSLTMPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARA
Sbjct: 1   MFPPLPLLSVLPSLTMPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARA 60

Query: 61  YDQAAILMSGRNAKTNFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLD 120
           YDQAAILMSGRNAKTNFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLD
Sbjct: 61  YDQAAILMSGRNAKTNFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLD 120

Query: 121 TENSNIGVWQKRAGPRSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPS 180
           TENSNIGVWQKRAGPRSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPS
Sbjct: 121 TENSNIGVWQKRAGPRSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPS 180

Query: 181 ELDEEERMVALQMVEELLHISYDEPNELFEIQQGKDVYL 220
           ELDEEERMVALQMVEELLHISYDEPNELFEIQQGKDVYL
Sbjct: 181 ELDEEERMVALQMVEELLHISYDEPNELFEIQQGKDVYL 219

[Close]

BLAST of Carg12130-RA vs. NCBI nr
Match: XP_022971945.1 (ethylene-responsive transcription factor WIN1-like [Cucurbita maxima])

HSP 1 Score: 432.6 bits (1111), Expect = 2.1e-117
Identity = 215/219 (98.17%), Postives = 219/219 (100.00%), Query Frame = 0


Query: 1   MFPPLPLLSVLPSLTMPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARA 60
           MFPPLPLLSVLPSLTMPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARA
Sbjct: 1   MFPPLPLLSVLPSLTMPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARA 60

Query: 61  YDQAAILMSGRNAKTNFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLD 120
           YDQAAILMSGRNAKTNFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLD
Sbjct: 61  YDQAAILMSGRNAKTNFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLD 120

Query: 121 TENSNIGVWQKRAGPRSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPS 180
           TENSNIGVWQKRAGPRSASSWVMTVELGNKKSHNNE++SLMGVVVADQSPVAAEVAEIPS
Sbjct: 121 TENSNIGVWQKRAGPRSASSWVMTVELGNKKSHNNEEESLMGVVVADQSPVAAEVAEIPS 180

Query: 181 ELDEEERMVALQMVEELLHISYDEPNELFEIQQGKDVYL 220
           ELDEEERMVALQMVEELLHISYDEPN++FEIQQGKDVYL
Sbjct: 181 ELDEEERMVALQMVEELLHISYDEPNQMFEIQQGKDVYL 219

[Close]

BLAST of Carg12130-RA vs. NCBI nr
Match: XP_022933544.1 (ethylene-responsive transcription factor WIN1-like [Cucurbita moschata])

HSP 1 Score: 409.8 bits (1052), Expect = 1.4e-110
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 0


Query: 16  MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT 75
           MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT
Sbjct: 1   MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT 60

Query: 76  NFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGP 135
           NFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGP
Sbjct: 61  NFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGP 120

Query: 136 RSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVE 195
           RSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVE
Sbjct: 121 RSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVE 180

Query: 196 ELLHISYDEPNELFEIQQGKDVYL 220
           ELLHISYDEPNELFEIQQGKDVYL
Sbjct: 181 ELLHISYDEPNELFEIQQGKDVYL 204

[Close]

BLAST of Carg12130-RA vs. NCBI nr
Match: KAG6587899.1 (Ethylene-responsive transcription factor WIN1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 409.1 bits (1050), Expect = 2.5e-110
Identity = 203/204 (99.51%), Postives = 204/204 (100.00%), Query Frame = 0


Query: 16  MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT 75
           MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT
Sbjct: 1   MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT 60

Query: 76  NFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGP 135
           NFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGP
Sbjct: 61  NFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGP 120

Query: 136 RSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVE 195
           RSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVE
Sbjct: 121 RSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVE 180

Query: 196 ELLHISYDEPNELFEIQQGKDVYL 220
           ELLHISYDEPNE+FEIQQGKDVYL
Sbjct: 181 ELLHISYDEPNEMFEIQQGKDVYL 204

[Close]

BLAST of Carg12130-RA vs. NCBI nr
Match: XP_023531753.1 (ethylene-responsive transcription factor WIN1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 407.9 bits (1047), Expect = 5.5e-110
Identity = 202/204 (99.02%), Postives = 204/204 (100.00%), Query Frame = 0


Query: 16  MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT 75
           MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT
Sbjct: 1   MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT 60

Query: 76  NFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGP 135
           NFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGP
Sbjct: 61  NFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGP 120

Query: 136 RSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVE 195
           RSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVE
Sbjct: 121 RSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVE 180

Query: 196 ELLHISYDEPNELFEIQQGKDVYL 220
           ELLHISYDEPN++FEIQQGKDVYL
Sbjct: 181 ELLHISYDEPNQMFEIQQGKDVYL 204

[Close]

BLAST of Carg12130-RA vs. ExPASy Swiss-Prot
Match: Q9XI33 (Ethylene-responsive transcription factor WIN1 OS=Arabidopsis thaliana OX=3702 GN=WIN1 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 5.2e-55
Identity = 116/199 (58.29%), Postives = 142/199 (71.36%), Query Frame = 0


Query: 16  MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT 75
           M + KKFRGVRQRHWGSWV+EIRHPLLKRRIWLGTFETAEEAARAYD+AA+LMSGRNAKT
Sbjct: 1   MVQTKKFRGVRQRHWGSWVAEIRHPLLKRRIWLGTFETAEEAARAYDEAAVLMSGRNAKT 60

Query: 76  NFPTTHD--------------TNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDT 135
           NFP  ++              ++  ++  S   LS IL AKL+KCC++PSPS+TCLRLDT
Sbjct: 61  NFPLNNNNTGETSEGKTDISASSTMSSSTSSSSLSSILSAKLRKCCKSPSPSLTCLRLDT 120

Query: 136 ENSNIGVWQKRAGPRSASSWVMTVELG-NKKSHNNEDDSLMGVVVADQSPVAAEVAEIPS 195
            +S+IGVWQKRAG +S SSWVMTVELG    S      +    ++A  + V  E+     
Sbjct: 121 ASSHIGVWQKRAGSKSDSSWVMTVELGPASSSQETTSKASQDAILAPTTEV--EIGGSRE 180

Query: 196 ELDEEERMVALQMVEELLH 200
           E+ +EE  VALQM+EELL+
Sbjct: 181 EVLDEEEKVALQMIEELLN 197

[Close]

BLAST of Carg12130-RA vs. ExPASy Swiss-Prot
Match: Q9LFN7 (Ethylene-responsive transcription factor SHINE 2 OS=Arabidopsis thaliana OX=3702 GN=SHN2 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.0e-50
Identity = 104/189 (55.03%), Postives = 142/189 (75.13%), Query Frame = 0


Query: 20  KKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKTNFP- 79
           +KFRGVRQR WGSWVSEIRHPLLKRR+WLGTFETAE AARAYDQAA+LM+G+NAKTNFP 
Sbjct: 5   RKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFETAEAAARAYDQAALLMNGQNAKTNFPV 64

Query: 80  ------TTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKR 139
                 + H  ++ +   SPK LSE+L AKL+K C+  +PS+TCLRLDT++S+IGVWQKR
Sbjct: 65  VKSEEGSDHVKDVNSPLMSPKSLSELLNAKLRKSCKDLTPSLTCLRLDTDSSHIGVWQKR 124

Query: 140 AGPRSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQ 199
           AG +++ +WVM +ELGN  + +  D   +G+   ++  V  E  E  + + +E+++ A++
Sbjct: 125 AGSKTSPTWVMRLELGNVVNESAVD---LGLTTMNKQNVEKEEEEEEAIISDEDQL-AME 184

Query: 200 MVEELLHIS 202
           M+EELL+ S
Sbjct: 185 MIEELLNWS 189

[Close]

BLAST of Carg12130-RA vs. ExPASy Swiss-Prot
Match: Q3E958 (Ethylene-responsive transcription factor SHINE 3 OS=Arabidopsis thaliana OX=3702 GN=SHN3 PE=2 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 4.0e-47
Identity = 98/185 (52.97%), Postives = 131/185 (70.81%), Query Frame = 0


Query: 20  KKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKTNFPT 79
           KKFRGVRQR WGSWVSEIRHPLLKRR+WLGTF+TAE AARAYDQAA+LM+G++AKTNFP 
Sbjct: 5   KKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAETAARAYDQAAVLMNGQSAKTNFPV 64

Query: 80  -----THDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAG 139
                ++   I +   SPK LSE+L AKL+K C+  +P +TCLRLD ++S+IGVWQKRAG
Sbjct: 65  IKSNGSNSLEINSALRSPKSLSELLNAKLRKNCKDQTPYLTCLRLDNDSSHIGVWQKRAG 124

Query: 140 PRSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMV 199
            +++ +WV  VELG+K +     D            +     ++ +E  +E+  +A+QM+
Sbjct: 125 SKTSPNWVKLVELGDKVNARPGGD------------IETNKMKVRNEDVQEDDQMAMQMI 177

[Close]

BLAST of Carg12130-RA vs. ExPASy Swiss-Prot
Match: Q94AW5 (Ethylene-responsive transcription factor ERF003 OS=Arabidopsis thaliana OX=3702 GN=ERF003 PE=1 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 3.3e-25
Identity = 83/180 (46.11%), Postives = 103/180 (57.22%), Query Frame = 0


Query: 20  KKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKTNFPT 79
           ++FRGVRQRHWGSWVSEIRHPLLK RIWLGTFETAE+AARAYD+AA LM G  A+TNFP 
Sbjct: 6   QRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRARTNFP- 65

Query: 80  THDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGPRSAS 139
            ++ N   T  S K LS  L AKL KC       M  L++ T+ +      + A  +SA 
Sbjct: 66  -YNPNAIPTSSS-KLLSATLTAKLHKC------YMASLQM-TKQTQTQTQTQTARSQSAD 125

Query: 140 SWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVEELLH 199
           S  +T      +SH N   +    +  +      +    P E D  E     QM+EELLH
Sbjct: 126 SDGVTA----NESHLNRGVTETTEIKWEDGNANMQQNFRPLEEDHIE-----QMIEELLH 166

[Close]

BLAST of Carg12130-RA vs. ExPASy Swiss-Prot
Match: P16146 (Protein PPLZ02 OS=Lupinus polyphyllus OX=3874 GN=PPLZ02 PE=2 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.2e-21
Identity = 53/87 (60.92%), Postives = 66/87 (75.86%), Query Frame = 0


Query: 20  KKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKTNFPT 79
           +++RG RQRHWGSWVSEIRH +LK RIW GTFE+AE+AARAYD+AA LM G  A+TNFP 
Sbjct: 6   QRYRGFRQRHWGSWVSEIRHSILKTRIWQGTFESAEDAARAYDEAARLMCGTRARTNFP- 65

Query: 80  THDTNITTTKDSPKGLSEILQAKLKKC 107
            ++ N + +  S K LS  L AKL +C
Sbjct: 66  -YNPNASQSSSS-KLLSATLIAKLHRC 89

[Close]

BLAST of Carg12130-RA vs. ExPASy TrEMBL
Match: A0A6J1I753 (ethylene-responsive transcription factor WIN1-like OS=Cucurbita maxima OX=3661 GN=LOC111470614 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.0e-117
Identity = 215/219 (98.17%), Postives = 219/219 (100.00%), Query Frame = 0


Query: 1   MFPPLPLLSVLPSLTMPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARA 60
           MFPPLPLLSVLPSLTMPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARA
Sbjct: 1   MFPPLPLLSVLPSLTMPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARA 60

Query: 61  YDQAAILMSGRNAKTNFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLD 120
           YDQAAILMSGRNAKTNFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLD
Sbjct: 61  YDQAAILMSGRNAKTNFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLD 120

Query: 121 TENSNIGVWQKRAGPRSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPS 180
           TENSNIGVWQKRAGPRSASSWVMTVELGNKKSHNNE++SLMGVVVADQSPVAAEVAEIPS
Sbjct: 121 TENSNIGVWQKRAGPRSASSWVMTVELGNKKSHNNEEESLMGVVVADQSPVAAEVAEIPS 180

Query: 181 ELDEEERMVALQMVEELLHISYDEPNELFEIQQGKDVYL 220
           ELDEEERMVALQMVEELLHISYDEPN++FEIQQGKDVYL
Sbjct: 181 ELDEEERMVALQMVEELLHISYDEPNQMFEIQQGKDVYL 219

[Close]

BLAST of Carg12130-RA vs. ExPASy TrEMBL
Match: A0A6J1F021 (ethylene-responsive transcription factor WIN1-like OS=Cucurbita moschata OX=3662 GN=LOC111440941 PE=4 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 7.0e-111
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 0


Query: 16  MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT 75
           MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT
Sbjct: 1   MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT 60

Query: 76  NFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGP 135
           NFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGP
Sbjct: 61  NFPTTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGP 120

Query: 136 RSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVE 195
           RSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVE
Sbjct: 121 RSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVE 180

Query: 196 ELLHISYDEPNELFEIQQGKDVYL 220
           ELLHISYDEPNELFEIQQGKDVYL
Sbjct: 181 ELLHISYDEPNELFEIQQGKDVYL 204

[Close]

BLAST of Carg12130-RA vs. ExPASy TrEMBL
Match: A0A1S3BA41 (ethylene-responsive transcription factor WIN1-like OS=Cucumis melo OX=3656 GN=LOC103487449 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.8e-83
Identity = 171/219 (78.08%), Postives = 185/219 (84.47%), Query Frame = 0


Query: 16  MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT 75
           MPK KKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAE+AA+AYDQAA+LMSGRNAKT
Sbjct: 1   MPKCKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEQAAKAYDQAAVLMSGRNAKT 60

Query: 76  NFPTTHD-----TNI-TTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVW 135
           NFPT+        NI TTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVW
Sbjct: 61  NFPTSSSNGETINNIDTTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVW 120

Query: 136 QKRAGPRSASSWVMTVELGNKK---------SHNNEDDSLMGVVVAD--QSPVAAEVAEI 195
           QKRAG +S S+WVMTVELGNKK         + NN D  +M VV +D  QSPV  EVAEI
Sbjct: 121 QKRAGQQSTSNWVMTVELGNKKRLSNENENENDNNADSDMMAVVASDHHQSPV-VEVAEI 180

Query: 196 PSELDEEERMVALQMVEELLHISYDEPNELFEIQQGKDV 218
           PSELDEEE+M+ALQMVEELLHI++D P E FEIQQGKD+
Sbjct: 181 PSELDEEEKMLALQMVEELLHINFD-PAEPFEIQQGKDI 217

[Close]

BLAST of Carg12130-RA vs. ExPASy TrEMBL
Match: A0A5D3C530 (Ethylene-responsive transcription factor WIN1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G001380 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 8.0e-83
Identity = 170/219 (77.63%), Postives = 184/219 (84.02%), Query Frame = 0


Query: 16  MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT 75
           MPK KKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAE+AA+AYDQAA+LMSGRNAKT
Sbjct: 1   MPKCKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEQAAKAYDQAAVLMSGRNAKT 60

Query: 76  NFPTTHD-----TNI-TTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVW 135
           NFPT+        NI TTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVW
Sbjct: 61  NFPTSSSNGETINNIDTTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVW 120

Query: 136 QKRAGPRSASSWVMTVELGNKK---------SHNNEDDSLMGVVVAD--QSPVAAEVAEI 195
           QKRAG +S S+WVMTVELGNKK         + NN D  +M VV +D  QSPV  EVAEI
Sbjct: 121 QKRAGQQSTSNWVMTVELGNKKRLSNENENENDNNADSDMMAVVASDHHQSPV-VEVAEI 180

Query: 196 PSELDEEERMVALQMVEELLHISYDEPNELFEIQQGKDV 218
           PSELDEEE+M+ LQMVEELLHI++D P E FEIQQGKD+
Sbjct: 181 PSELDEEEKMLTLQMVEELLHINFD-PAEPFEIQQGKDI 217

[Close]

BLAST of Carg12130-RA vs. ExPASy TrEMBL
Match: A0A5A7TUK2 (Ethylene-responsive transcription factor WIN1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold216G001140 PE=4 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 1.8e-82
Identity = 170/219 (77.63%), Postives = 184/219 (84.02%), Query Frame = 0


Query: 16  MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT 75
           MPK KKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAE+AA+AYDQAA+LMSGRNAKT
Sbjct: 1   MPKCKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEQAAKAYDQAAVLMSGRNAKT 60

Query: 76  NFPTTHD-----TNI-TTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVW 135
           NFPT+        NI TTTKDSPK LSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVW
Sbjct: 61  NFPTSSSNGETINNIDTTTKDSPKDLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVW 120

Query: 136 QKRAGPRSASSWVMTVELGNKK---------SHNNEDDSLMGVVVAD--QSPVAAEVAEI 195
           QKRAG +S S+WVMTVELGNKK         + NN D  +M VV +D  QSPV  EVAEI
Sbjct: 121 QKRAGQQSTSNWVMTVELGNKKRLSNENENENDNNADSDMMAVVASDHHQSPV-VEVAEI 180

Query: 196 PSELDEEERMVALQMVEELLHISYDEPNELFEIQQGKDV 218
           PSELDEEE+M+ALQMVEELLHI++D P E FEIQQGKD+
Sbjct: 181 PSELDEEEKMLALQMVEELLHINFD-PAEPFEIQQGKDI 217

[Close]

BLAST of Carg12130-RA vs. TAIR 10
Match: AT1G15360.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 215.7 bits (548), Expect = 3.7e-56
Identity = 116/199 (58.29%), Postives = 142/199 (71.36%), Query Frame = 0


Query: 16  MPKFKKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKT 75
           M + KKFRGVRQRHWGSWV+EIRHPLLKRRIWLGTFETAEEAARAYD+AA+LMSGRNAKT
Sbjct: 1   MVQTKKFRGVRQRHWGSWVAEIRHPLLKRRIWLGTFETAEEAARAYDEAAVLMSGRNAKT 60

Query: 76  NFPTTHD--------------TNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDT 135
           NFP  ++              ++  ++  S   LS IL AKL+KCC++PSPS+TCLRLDT
Sbjct: 61  NFPLNNNNTGETSEGKTDISASSTMSSSTSSSSLSSILSAKLRKCCKSPSPSLTCLRLDT 120

Query: 136 ENSNIGVWQKRAGPRSASSWVMTVELG-NKKSHNNEDDSLMGVVVADQSPVAAEVAEIPS 195
            +S+IGVWQKRAG +S SSWVMTVELG    S      +    ++A  + V  E+     
Sbjct: 121 ASSHIGVWQKRAGSKSDSSWVMTVELGPASSSQETTSKASQDAILAPTTEV--EIGGSRE 180

Query: 196 ELDEEERMVALQMVEELLH 200
           E+ +EE  VALQM+EELL+
Sbjct: 181 EVLDEEEKVALQMIEELLN 197

[Close]

BLAST of Carg12130-RA vs. TAIR 10
Match: AT5G11190.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 201.4 bits (511), Expect = 7.2e-52
Identity = 104/189 (55.03%), Postives = 142/189 (75.13%), Query Frame = 0


Query: 20  KKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKTNFP- 79
           +KFRGVRQR WGSWVSEIRHPLLKRR+WLGTFETAE AARAYDQAA+LM+G+NAKTNFP 
Sbjct: 5   RKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFETAEAAARAYDQAALLMNGQNAKTNFPV 64

Query: 80  ------TTHDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKR 139
                 + H  ++ +   SPK LSE+L AKL+K C+  +PS+TCLRLDT++S+IGVWQKR
Sbjct: 65  VKSEEGSDHVKDVNSPLMSPKSLSELLNAKLRKSCKDLTPSLTCLRLDTDSSHIGVWQKR 124

Query: 140 AGPRSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQ 199
           AG +++ +WVM +ELGN  + +  D   +G+   ++  V  E  E  + + +E+++ A++
Sbjct: 125 AGSKTSPTWVMRLELGNVVNESAVD---LGLTTMNKQNVEKEEEEEEAIISDEDQL-AME 184

Query: 200 MVEELLHIS 202
           M+EELL+ S
Sbjct: 185 MIEELLNWS 189

[Close]

BLAST of Carg12130-RA vs. TAIR 10
Match: AT5G25390.2 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 189.5 bits (480), Expect = 2.9e-48
Identity = 98/185 (52.97%), Postives = 131/185 (70.81%), Query Frame = 0


Query: 20  KKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKTNFPT 79
           KKFRGVRQR WGSWVSEIRHPLLKRR+WLGTF+TAE AARAYDQAA+LM+G++AKTNFP 
Sbjct: 5   KKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAETAARAYDQAAVLMNGQSAKTNFPV 64

Query: 80  -----THDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAG 139
                ++   I +   SPK LSE+L AKL+K C+  +P +TCLRLD ++S+IGVWQKRAG
Sbjct: 65  IKSNGSNSLEINSALRSPKSLSELLNAKLRKNCKDQTPYLTCLRLDNDSSHIGVWQKRAG 124

Query: 140 PRSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMV 199
            +++ +WV  VELG+K +     D            +     ++ +E  +E+  +A+QM+
Sbjct: 125 SKTSPNWVKLVELGDKVNARPGGD------------IETNKMKVRNEDVQEDDQMAMQMI 177

[Close]

BLAST of Carg12130-RA vs. TAIR 10
Match: AT5G25390.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 178.7 bits (452), Expect = 5.0e-45
Identity = 95/185 (51.35%), Postives = 128/185 (69.19%), Query Frame = 0


Query: 20  KKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKTNFPT 79
           KKFRGVRQR WGSWVSEIRHPLL   +WLGTF+TAE AARAYDQAA+LM+G++AKTNFP 
Sbjct: 5   KKFRGVRQRQWGSWVSEIRHPLL---VWLGTFDTAETAARAYDQAAVLMNGQSAKTNFPV 64

Query: 80  -----THDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAG 139
                ++   I +   SPK LSE+L AKL+K C+  +P +TCLRLD ++S+IGVWQKRAG
Sbjct: 65  IKSNGSNSLEINSALRSPKSLSELLNAKLRKNCKDQTPYLTCLRLDNDSSHIGVWQKRAG 124

Query: 140 PRSASSWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMV 199
            +++ +WV  VELG+K +     D            +     ++ +E  +E+  +A+QM+
Sbjct: 125 SKTSPNWVKLVELGDKVNARPGGD------------IETNKMKVRNEDVQEDDQMAMQMI 174

[Close]

BLAST of Carg12130-RA vs. TAIR 10
Match: AT5G25190.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 2.3e-26
Identity = 83/180 (46.11%), Postives = 103/180 (57.22%), Query Frame = 0


Query: 20  KKFRGVRQRHWGSWVSEIRHPLLKRRIWLGTFETAEEAARAYDQAAILMSGRNAKTNFPT 79
           ++FRGVRQRHWGSWVSEIRHPLLK RIWLGTFETAE+AARAYD+AA LM G  A+TNFP 
Sbjct: 6   QRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRARTNFP- 65

Query: 80  THDTNITTTKDSPKGLSEILQAKLKKCCRTPSPSMTCLRLDTENSNIGVWQKRAGPRSAS 139
            ++ N   T  S K LS  L AKL KC       M  L++ T+ +      + A  +SA 
Sbjct: 66  -YNPNAIPTSSS-KLLSATLTAKLHKC------YMASLQM-TKQTQTQTQTQTARSQSAD 125

Query: 140 SWVMTVELGNKKSHNNEDDSLMGVVVADQSPVAAEVAEIPSELDEEERMVALQMVEELLH 199
           S  +T      +SH N   +    +  +      +    P E D  E     QM+EELLH
Sbjct: 126 SDGVTA----NESHLNRGVTETTEIKWEDGNANMQQNFRPLEEDHIE-----QMIEELLH 166

The following BLAST results are available for this feature:

BLAST of Carg12130-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021785.1	6.5e-119	100.00	Ethylene-responsive transcription factor WIN1, partial [Cucurbita argyrosperma s...	[more]
XP_022971945.1	2.1e-117	98.17	ethylene-responsive transcription factor WIN1-like [Cucurbita maxima]	[more]
XP_022933544.1	1.4e-110	100.00	ethylene-responsive transcription factor WIN1-like [Cucurbita moschata]	[more]
KAG6587899.1	2.5e-110	99.51	Ethylene-responsive transcription factor WIN1, partial [Cucurbita argyrosperma s...	[more]
XP_023531753.1	5.5e-110	99.02	ethylene-responsive transcription factor WIN1-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg12130-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XI33	5.2e-55	58.29	Ethylene-responsive transcription factor WIN1 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q9LFN7	1.0e-50	55.03	Ethylene-responsive transcription factor SHINE 2 OS=Arabidopsis thaliana OX=3702...	[more]
Q3E958	4.0e-47	52.97	Ethylene-responsive transcription factor SHINE 3 OS=Arabidopsis thaliana OX=3702...	[more]
Q94AW5	3.3e-25	46.11	Ethylene-responsive transcription factor ERF003 OS=Arabidopsis thaliana OX=3702 ...	[more]
P16146	2.2e-21	60.92	Protein PPLZ02 OS=Lupinus polyphyllus OX=3874 GN=PPLZ02 PE=2 SV=1	[more]

BLAST of Carg12130-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I753	1.0e-117	98.17	ethylene-responsive transcription factor WIN1-like OS=Cucurbita maxima OX=3661 G...	[more]
A0A6J1F021	7.0e-111	100.00	ethylene-responsive transcription factor WIN1-like OS=Cucurbita moschata OX=3662...	[more]
A0A1S3BA41	2.8e-83	78.08	ethylene-responsive transcription factor WIN1-like OS=Cucumis melo OX=3656 GN=LO...	[more]
A0A5D3C530	8.0e-83	77.63	Ethylene-responsive transcription factor WIN1-like OS=Cucumis melo var. makuwa O...	[more]
A0A5A7TUK2	1.8e-82	77.63	Ethylene-responsive transcription factor WIN1-like OS=Cucumis melo var. makuwa O...	[more]

BLAST of Carg12130-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G15360.1	3.7e-56	58.29	Integrase-type DNA-binding superfamily protein	[more]
AT5G11190.1	7.2e-52	55.03	Integrase-type DNA-binding superfamily protein	[more]
AT5G25390.2	2.9e-48	52.97	Integrase-type DNA-binding superfamily protein	[more]
AT5G25390.1	5.0e-45	51.35	Integrase-type DNA-binding superfamily protein	[more]
AT5G25190.1	2.3e-26	46.11	Integrase-type DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 22..33 score: 61.21 coord: 44..60 score: 71.66
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 21..84 e-value: 1.3E-38 score: 144.2
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 21..69 e-value: 1.4E-12 score: 47.6
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 21..78 score: 21.878006
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 20..78 e-value: 1.70142E-35 score: 118.143
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 20..80 e-value: 9.7E-27 score: 94.9
None	No IPR available	PANTHER	PTHR31194	SHN SHINE , DNA BINDING / TRANSCRIPTION FACTOR	coord: 16..205
None	No IPR available	PANTHER	PTHR31194:SF49	AP2-EREBP TRANSCRIPTION FACTOR	coord: 16..205
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 21..79

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg12130	Carg12130	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12130-RA:1	Carg12130-RA:1	exon
Carg12130-RA:2	Carg12130-RA:2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg12130-RA:cds	Carg12130-RA:cds	CDS
Carg12130-RA:cds	Carg12130-RA:cds_2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg12130-RA	Carg12130-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0004672	protein kinase activity