Carg10813-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg10813-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Phospholipase A2 family protein
Location	Carg_Chr05: 11965472 .. 11968695 (-)
Sequence length	967
RNA-Seq Expression	Carg10813-RA
Synteny	Carg10813-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACGAAGAGTTCATATCGCAGAGCCAATCGGACGGGAGAGACGCGGTACAGAAAAGCTCCGCCAAAGGAATCTGTTTGTGGTCTGAAGAAGGCGACTGAAGATGGTCTGCAATACCAGCATTTCCCTTCGCAAACAAGTCGCATCGGCGATTTTCCTTCTCCTCGTTCTACTCACCGTCGTCTCCGAGTGTTCCAGTCAGGTAATAATCTGCTCCTTTTGTTATTTTAAGAACTGATGCTGGAGAATTTGGATCTTAGATTCGTACATTTGGTTCTTCAGTCTCCTTCGTTTGTTTCTTGATGTGAAATTCAATTTGTTTGGCTTCTATTCAACGATTTGGATGTTAACGTCAAATTTTCGTTGGATGATAGGTTGAATGCAGTAGAACTTGTGCTTCAGAGAAATGCAATAGTGAGTATATTCTGTGTTTTTATATGAAATTCGTCATCTGTTGTTATTATTTTTTGAATTTTAATTTCTCATCAAATATTTATTTTGTTTGTTTATCTGAAAATTAGCTGTAGGGATTCGGTACGGTAAGTTCTGCGGAGTAGGATGGACGGGTTGTGCTGGTGAAAAGCCTTGCGATGAGCTTGATGCTTGTTGCAAAGATCATGACGAATGTGTTGAATATAAAGGTTATTGCCAATCTATAACTACATGCCTTATTGTTTCCTTTCTATACTTCGCACTGTTTGCGATTCCTCATCATTATACTTGCATAGAAAAATTTCCCATCGATCTATATTCCTGTTTTAGTTTTAAGTTGTTGTGAGACCAATGGGGTGAAATCTGGGCATCCTGTTGTTCAATGAACTCTTTTTTCGGGCATTATTGGTATATTCTCTGCTGCATGCTTGGAAAAAGATACGTATGAGATTCAAATATTCGACTACGATTTCTACCCTTTGAGAATAGAAGAATCGAAATTTGCTTTCCCAACAAGATCTTTCTCAATGAGGCTACCCCTTGTTTTCATGCCCCTTAAATCATTTCCGTTCTCAAATAAATTCCCGTTGAATACTTCATCCTCGGCCTCAAATTTAATTTAAAAAGGGCGACAATCCTCGCATCTTGACTGAACTCAGCGGGTCGAACCATTCACATGACTCCTCGATAGTATGATAATGTCCACTTTGGATATAAGTCTCATACCAATGGAGATGGTTTTCTTTATTTTCCCATGATCTTCCCCCTTCCGTAGTCAATGTGAACCTTTGGTCGCACTTTCTACAATCTTCCACCAAATAAAGTGCCATGGAGCCTCCCCGTTCTAGGGCTCACCATCTGTTTGTTTGGCATCCAAAGACTCTTTCAACATGGCTAAGTCAAGAAGGTGATTATGATACCATTACAAGAGTTAGAAAGAGAGAGAAGAGAATGTGATCATAAAAGTACTCCGCTGTTCAACATTAGTGGTTGGAGAAGTAATCCTATTTATAGGACTCTCTGAAACAATATTAACTTACGGTACGACTGTATACTAAAGCAGTATTAGCAAAGATACATCGTATCAAGCTCTTAACAACCAATGAGTTAAGTCTTAACACCTTTGGCTTCTAAATTCTTAAACAATGTCATCAAACTCCAAGCCACCAGTTTCTCCCTACCAACATACATCATCAATGGATCCTAACTAAACCTAACCACGGAGAATAGCTACTTCCATTCGAGGAAACTGTTGTCCTATAGTTTAGTCCCGACTTGTGAGCTGCTGGGTTGGCCACATTAATAATGTTGGAGAGGTTAATGAGTTGTTTTTCTAAACCCGAGTCCTCCCAGTTGAATTTTCTTATGGATGATGTACTGCCATTTCCTCTGTCATATGCTTACCTGTGAGAAGCTTCATCAGATTCTTGATACCTCAAGGTTAGGGTGCTTCCTTATACTGCCAGCGTGTAGAGGCTGATTACCATCCTCAAAATGCTTATGCGGTATAATAAACACTTTTGGTTGTTATTAGATTTTTTTGACAGAAGACAAAGAATGGTTGAATAGTCTATTGTGTTCACAACTTTTAAGTCGAATCCTTCGTGTCGTTAACTTCGGAGTAGGATGACAAACTGTCACATGTTAACTCCCTTTTCAAATGCATGGCAGTAAGGACGTTGAACACTTTTGCCTCCCTTGTAGTGATCATTGAGTGGATTACATCAGTAAATGCCTACGAATAGTCAGTTATTTATATTATGCACATAGTAGACTTCTGTTTATTACGGATCAGTAAAGCTATTGGCCTCCTGGCCATTGAAAAAAAAAATTAGATACAAAATTGGAGATATTAAGGCATGGCCAATCGGCATATTAAAACAAAGAGGAGACACTAGACAGTCACCAATGTTTGGTGTTGCTAAAGTCTATATCTTAGATCTTAGATGTCTTTTATTTATCTGAGATACTACACATTTCAAACTTTGTAGCACTAGATTACAGGAGACCAAGCAATTTACATATATATATACATATATTGGTTCATTTTTGCAATATTGTTGTACCATGTTTTCTTTTTGTTCAATTATGATTTTGGGTGGAATTTTAAGAGTTGGAACTTGAGAGTCACTCTGTTGAATATGTTTTGTTTCAAGACCAATAACAGATATCACATGGGTTTTTCAGGTTTAACCAATATTAAATGTCACATAAAGTTCAAGGATTGTATTACGAAATTGCAGAAATCTGGGATGGCTGGTTTCTCACAGCAGTGCCCTTATTCCATAGCTATTCCTACAATGGTAAATGGCATGGATATAGCCATCATGTTCAGCCAGTTTAGTAGCGGAAAGTTTGAGCTCTGATAGCCACTTTGGTAACTCAACTCGCTCCATCATCCCCGCCCACAATTCATGCCATCAATTATATTGCTCATACTCCCCCAAAACAAGGTGGATAGCTTCACACCACTGATTCATTCTTTTAAATAATATTCTATTGGATTAAGAAATCTCAAACAGAGGATTTGATCCTCATTTTTTTGTCCCCTTTAACTGTTATTTGGACCATCTGCACTTTAAGGATGTTTTTAGCATAGTGAAAGAAGATTCCAATAGGCCAAATTATTTCATGTTTGATGCTTCCTCAATAATCAATAGCTGTTAGGTTTTGGTTTCCCTCCTTATTGTGTGTCAAATACTCCATTTTTAAGGAGTCACCCAATGTGTGGGCAGACTGTTGCAAGTTATCTTCTGTTGTTGCCTTTTCGTTTGAAGTTGAACAATCACAGTTTA

mRNA sequence

ACGAAGAGTTCATATCGCAGAGCCAATCGGACGGGAGAGACGCGGTACAGAAAAGCTCCGCCAAAGGAATCTGTTTGTGGTCTGAAGAAGGCGACTGAAGATGGTCTGCAATACCAGCATTTCCCTTCGCAAACAAGTCGCATCGGCGATTTTCCTTCTCCTCGTTCTACTCACCGTCGTCTCCGAGTGTTCCAGTCAGGTTGAATGCAGTAGAACTTGTGCTTCAGAGAAATGCAATACTGTAGGGATTCGGTACGGTAAGTTCTGCGGAGTAGGATGGACGGGTTGTGCTGGTGAAAAGCCTTGCGATGAGCTTGATGCTTGTTGCAAAGATCATGACGAATGTGTTGAATATAAAGGTTTAACCAATATTAAATGTCACATAAAGTTCAAGGATTGTATTACGAAATTGCAGAAATCTGGGATGGCTGGTTTCTCACAGCAGTGCCCTTATTCCATAGCTATTCCTACAATGGTAAATGGCATGGATATAGCCATCATGTTCAGCCAGTTTAGTAGCGGAAAGTTTGAGCTCTGATAGCCACTTTGGTAACTCAACTCGCTCCATCATCCCCGCCCACAATTCATGCCATCAATTATATTGCTCATACTCCCCCAAAACAAGGTGGATAGCTTCACACCACTGATTCATTCTTTTAAATAATATTCTATTGGATTAAGAAATCTCAAACAGAGGATTTGATCCTCATTTTTTTGTCCCCTTTAACTGTTATTTGGACCATCTGCACTTTAAGGATGTTTTTAGCATAGTGAAAGAAGATTCCAATAGGCCAAATTATTTCATGTTTGATGCTTCCTCAATAATCAATAGCTGTTAGGTTTTGGTTTCCCTCCTTATTGTGTGTCAAATACTCCATTTTTAAGGAGTCACCCAATGTGTGGGCAGACTGTTGCAAGTTATCTTCTGTTGTTGCCTTTTCGTTTGAAGTTGAACAATCACAGTTTA

Coding sequence (CDS)

ATGGTCTGCAATACCAGCATTTCCCTTCGCAAACAAGTCGCATCGGCGATTTTCCTTCTCCTCGTTCTACTCACCGTCGTCTCCGAGTGTTCCAGTCAGGTTGAATGCAGTAGAACTTGTGCTTCAGAGAAATGCAATACTGTAGGGATTCGGTACGGTAAGTTCTGCGGAGTAGGATGGACGGGTTGTGCTGGTGAAAAGCCTTGCGATGAGCTTGATGCTTGTTGCAAAGATCATGACGAATGTGTTGAATATAAAGGTTTAACCAATATTAAATGTCACATAAAGTTCAAGGATTGTATTACGAAATTGCAGAAATCTGGGATGGCTGGTTTCTCACAGCAGTGCCCTTATTCCATAGCTATTCCTACAATGGTAAATGGCATGGATATAGCCATCATGTTCAGCCAGTTTAGTAGCGGAAAGTTTGAGCTCTGA

Protein sequence

MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSIAIPTMVNGMDIAIMFSQFSSGKFEL

Homology

[Close]

BLAST of Carg10813-RA vs. NCBI nr
Match: KAG7030479.1 (putative phospholipase A2-like 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 302.0 bits (772), Expect = 2.8e-78
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW 60
           MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW
Sbjct: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW 60

Query: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120
           TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI
Sbjct: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120

Query: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 146
           AIPTMVNGMDIAIMFSQFSSGKFEL
Sbjct: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 145

[Close]

BLAST of Carg10813-RA vs. NCBI nr
Match: XP_022946468.1 (probable phospholipase A2 homolog 1 [Cucurbita moschata])

HSP 1 Score: 300.8 bits (769), Expect = 6.2e-78
Identity = 144/145 (99.31%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW 60
           MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQV+CSRTCASEKCNTVGIRYGKFCGVGW
Sbjct: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVQCSRTCASEKCNTVGIRYGKFCGVGW 60

Query: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120
           TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI
Sbjct: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120

Query: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 146
           AIPTMVNGMDIAIMFSQFSSGKFEL
Sbjct: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 145

[Close]

BLAST of Carg10813-RA vs. NCBI nr
Match: XP_022999541.1 (probable phospholipase A2 homolog 1 [Cucurbita maxima])

HSP 1 Score: 295.8 bits (756), Expect = 2.0e-76
Identity = 141/145 (97.24%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW 60
           MVCNTSISLRK VASAIFLLLVLL+VVSECSSQVECSRTC SEKCNTVGIRYGKFCGVGW
Sbjct: 1   MVCNTSISLRKHVASAIFLLLVLLSVVSECSSQVECSRTCVSEKCNTVGIRYGKFCGVGW 60

Query: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120
           TGCAGEKPCD+LDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI
Sbjct: 61  TGCAGEKPCDDLDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120

Query: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 146
           AIPTMVNGMDIAIMFSQFSSGKFEL
Sbjct: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 145

[Close]

BLAST of Carg10813-RA vs. NCBI nr
Match: XP_023545532.1 (probable phospholipase A2 homolog 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 292.4 bits (747), Expect = 2.2e-75
Identity = 140/145 (96.55%), Postives = 141/145 (97.24%), Query Frame = 0


Query: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW 60
           MVCN SISLRK VASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW
Sbjct: 1   MVCNISISLRKHVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW 60

Query: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120
           TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCH KFK CITK+QKSGMAGFSQQCPYSI
Sbjct: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHTKFKSCITKVQKSGMAGFSQQCPYSI 120

Query: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 146
           AIPTMVNGMDIAIMFSQFSSGKFEL
Sbjct: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 145

[Close]

BLAST of Carg10813-RA vs. NCBI nr
Match: KAG6599502.1 (60S ribosomal protein L28-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 263.8 bits (673), Expect = 8.5e-67
Identity = 125/125 (100.00%), Postives = 125/125 (100.00%), Query Frame = 0


Query: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW 60
           MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW
Sbjct: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW 60

Query: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120
           TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI
Sbjct: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120

Query: 121 AIPTM 126
           AIPTM
Sbjct: 121 AIPTM 125

[Close]

BLAST of Carg10813-RA vs. ExPASy Swiss-Prot
Match: Q9XG80 (Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-I PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 8.3e-41
Identity = 68/110 (61.82%), Postives = 92/110 (83.64%), Query Frame = 0


Query: 36  CSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHI 95
           CSR+CA+  C++VGIRYGK+CGVGW+GC GE+PCD+LDACC+DHD CV+ KGL ++KCH 
Sbjct: 29  CSRSCAALNCDSVGIRYGKYCGVGWSGCDGEEPCDDLDACCRDHDHCVDKKGLMSVKCHE 88

Query: 96  KFKDCITKLQKSGMAGFSQQCPYSIAIPTMVNGMDIAIMFSQFSSGKFEL 146
           KFK+C+ K++K+G  GFS++CPY +A+ TM +GMD+AIM SQ  + K EL
Sbjct: 89  KFKNCMRKVKKAGKIGFSRKCPYEMAMATMTSGMDMAIMLSQLGTQKLEL 138

[Close]

BLAST of Carg10813-RA vs. ExPASy Swiss-Prot
Match: Q8GZB4 (Phospholipase A2-beta OS=Arabidopsis thaliana OX=3702 GN=PLA2-BETA PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.4e-35
Identity = 67/132 (50.76%), Postives = 92/132 (69.70%), Query Frame = 0


Query: 15  SAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDA 74
           +A F  +V + +V    S+ EC+RTC ++ C+T+ IRYGK+CG+G +GC GE+PCD+LDA
Sbjct: 11  AAAFFAIVFVVLVGVARSE-ECTRTCIAQNCDTLSIRYGKYCGIGHSGCPGEEPCDDLDA 70

Query: 75  CCKDHDECVEYKGLTNIKCHIKFKDCITKL------QKSGMAGFSQQCPYSIAIPTMVNG 134
           CCK HD CVE  G+TNI CH KF+ C+ +L       K+   GFS +CPYS+ IPT+  G
Sbjct: 71  CCKIHDHCVELNGMTNISCHKKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQG 130

Query: 135 MDIAIMFSQFSS 141
           MDI I+FSQ  +
Sbjct: 131 MDIGILFSQLGN 141

[Close]

BLAST of Carg10813-RA vs. ExPASy Swiss-Prot
Match: Q9M0D7 (Phospholipase A2-gamma OS=Arabidopsis thaliana OX=3702 GN=PLA2-GAMMA PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.4e-35
Identity = 69/121 (57.02%), Postives = 85/121 (70.25%), Query Frame = 0


Query: 22  VLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDACCKDHDE 81
           +LL VV   SSQ +CS TC ++ CN++GIRYGK+CG+G+ GC GE PCD+LDACC  HD 
Sbjct: 18  LLLAVV---SSQEKCSNTCIAQNCNSLGIRYGKYCGIGYFGCPGEPPCDDLDACCMTHDN 77

Query: 82  CVEYKGLTNIKCHIKFKDCITKLQKS------GMAGFSQQCPYSIAIPTMVNGMDIAIMF 137
           CV+ KG+T + CH +FK C+ KL KS         GFS QCPYSI IPT+ NGMD  I F
Sbjct: 78  CVDLKGMTYVNCHKQFKRCVNKLSKSIKHSNGEKIGFSTQCPYSIVIPTVFNGMDYGIFF 135

[Close]

BLAST of Carg10813-RA vs. ExPASy Swiss-Prot
Match: Q8GV50 (Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 1.2e-31
Identity = 60/134 (44.78%), Postives = 89/134 (66.42%), Query Frame = 0


Query: 9   LRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKP 68
           +R    + + L L +  +++   SQ +CS+TC ++KCN +GIRYGK+CG+G+ GC GE P
Sbjct: 2   IRGGALTHVALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLGIRYGKYCGIGYFGCPGEPP 61

Query: 69  CDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKS------GMAGFSQQCPYSIAI 128
           CD+LD CC  HD CV+ KG+T + CH +F+ C+ +L++S         GFS++CPYS  I
Sbjct: 62  CDDLDDCCMTHDNCVDLKGMTYVDCHKQFQRCVNELKQSIQESNNQKVGFSKECPYSTVI 121

Query: 129 PTMVNGMDIAIMFS 137
           PT+  GM+  I FS
Sbjct: 122 PTVYRGMNYGIFFS 135

[Close]

BLAST of Carg10813-RA vs. ExPASy Swiss-Prot
Match: Q10E50 (Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 7.1e-16
Identity = 36/83 (43.37%), Postives = 53/83 (63.86%), Query Frame = 0


Query: 36  CSRTCASEKCNTVG-IRYGKFCGVGWTGCAGEKPCDELDACCKDHDECVEYKG-LTNIKC 95
           CSRTC S+ C T   +RYGK+CG+ ++GC GE+PCDELDACC  HD CV+ K    +  C
Sbjct: 55  CSRTCESDHCTTPPLLRYGKYCGILYSGCPGEQPCDELDACCMHHDNCVQAKNDYLSTAC 114

Query: 96  HIKFKDCITKLQKSGMAGFSQQC 117
           + +  +C+ +L++        +C
Sbjct: 115 NEELLECLARLREGSSTFQGNKC 137

[Close]

BLAST of Carg10813-RA vs. ExPASy TrEMBL
Match: A0A6J1G3Y1 (probable phospholipase A2 homolog 1 OS=Cucurbita moschata OX=3662 GN=LOC111450518 PE=4 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 3.0e-78
Identity = 144/145 (99.31%), Postives = 145/145 (100.00%), Query Frame = 0


Query: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW 60
           MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQV+CSRTCASEKCNTVGIRYGKFCGVGW
Sbjct: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVQCSRTCASEKCNTVGIRYGKFCGVGW 60

Query: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120
           TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI
Sbjct: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120

Query: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 146
           AIPTMVNGMDIAIMFSQFSSGKFEL
Sbjct: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 145

[Close]

BLAST of Carg10813-RA vs. ExPASy TrEMBL
Match: A0A6J1KDD2 (probable phospholipase A2 homolog 1 OS=Cucurbita maxima OX=3661 GN=LOC111493870 PE=4 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 9.7e-77
Identity = 141/145 (97.24%), Postives = 143/145 (98.62%), Query Frame = 0


Query: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGW 60
           MVCNTSISLRK VASAIFLLLVLL+VVSECSSQVECSRTC SEKCNTVGIRYGKFCGVGW
Sbjct: 1   MVCNTSISLRKHVASAIFLLLVLLSVVSECSSQVECSRTCVSEKCNTVGIRYGKFCGVGW 60

Query: 61  TGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120
           TGCAGEKPCD+LDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI
Sbjct: 61  TGCAGEKPCDDLDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSI 120

Query: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 146
           AIPTMVNGMDIAIMFSQFSSGKFEL
Sbjct: 121 AIPTMVNGMDIAIMFSQFSSGKFEL 145

[Close]

BLAST of Carg10813-RA vs. ExPASy TrEMBL
Match: A0A6J1DMI5 (probable phospholipase A2 homolog 1 OS=Momordica charantia OX=3673 GN=LOC111021924 PE=4 SV=1)

HSP 1 Score: 219.5 bits (558), Expect = 8.9e-54
Identity = 104/148 (70.27%), Postives = 120/148 (81.08%), Query Frame = 0


Query: 1   MVCNTSISLRKQVASAIFLLLVLLTVVSECS---SQVECSRTCASEKCNTVGIRYGKFCG 60
           MV + SI +RK  +S   L+L+ L+VVSECS   S+VECSRTC +  CNTVGIRYGKFCG
Sbjct: 1   MVRHPSIPIRKHFSSPFLLVLLFLSVVSECSSNESRVECSRTCVAINCNTVGIRYGKFCG 60

Query: 61  VGWTGCAGEKPCDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCP 120
           VGWTGCAGEKPCD+LDACCK HDECVE KGLTNIKCH KFK CI K+QKSG AGFS++CP
Sbjct: 61  VGWTGCAGEKPCDDLDACCKVHDECVERKGLTNIKCHEKFKSCIKKVQKSGKAGFSEECP 120

Query: 121 YSIAIPTMVNGMDIAIMFSQFSSGKFEL 146
           YS A+P MV GMD+AI+FSQ  + K EL
Sbjct: 121 YSTAVPIMVQGMDLAIVFSQLGNSKLEL 148

[Close]

BLAST of Carg10813-RA vs. ExPASy TrEMBL
Match: A0A1S3C986 (probable phospholipase A2 homolog 1 OS=Cucumis melo OX=3656 GN=LOC103498425 PE=4 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.2e-53
Identity = 101/133 (75.94%), Postives = 114/133 (85.71%), Query Frame = 0


Query: 16  AIFLLLVLLTVVSECS---SQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDEL 75
           +I LLL+LLTVVS+CS   S+VECSRTC +  CNTVGIRYGKFCGVGWTGCAGEKPCD+L
Sbjct: 8   SILLLLLLLTVVSDCSNNESRVECSRTCVAVNCNTVGIRYGKFCGVGWTGCAGEKPCDDL 67

Query: 76  DACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSIAIPTMVNGMDIA 135
           DACCK HDECVE KGLTN+KCH KFK CI K+QKSG  GFS++CPYS A+PTMV GMD+A
Sbjct: 68  DACCKVHDECVERKGLTNVKCHEKFKSCIKKVQKSGKVGFSKECPYSTAVPTMVQGMDLA 127

Query: 136 IMFSQFSSGKFEL 146
           IMFSQF + K EL
Sbjct: 128 IMFSQFGNSKLEL 140

[Close]

BLAST of Carg10813-RA vs. ExPASy TrEMBL
Match: A0A0A0LFR9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000560 PE=4 SV=1)

HSP 1 Score: 213.4 bits (542), Expect = 6.3e-52
Identity = 99/133 (74.44%), Postives = 111/133 (83.46%), Query Frame = 0


Query: 16  AIFLLLVLLTVVSECS---SQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDEL 75
           +I LLL+LLTVVS CS   S+VECSRTC +  CNTVGIRYGKFCGVGWTGCAGEKPCD+L
Sbjct: 8   SILLLLLLLTVVSHCSNNESRVECSRTCVAINCNTVGIRYGKFCGVGWTGCAGEKPCDDL 67

Query: 76  DACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKSGMAGFSQQCPYSIAIPTMVNGMDIA 135
           DACCK HDECVE KGLTN+KCH KFK CI K+QKSG  GFS  CPYS A+PTMV GM++A
Sbjct: 68  DACCKVHDECVERKGLTNVKCHEKFKSCIKKVQKSGKVGFSNDCPYSTAVPTMVQGMNLA 127

Query: 136 IMFSQFSSGKFEL 146
           IMFS+F + K EL
Sbjct: 128 IMFSKFGNSKLEL 140

[Close]

BLAST of Carg10813-RA vs. TAIR 10
Match: AT2G19690.1 (phospholipase A2-beta )

HSP 1 Score: 150.6 bits (379), Expect = 9.7e-37
Identity = 67/132 (50.76%), Postives = 92/132 (69.70%), Query Frame = 0


Query: 15  SAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDA 74
           +A F  +V + +V    S+ EC+RTC ++ C+T+ IRYGK+CG+G +GC GE+PCD+LDA
Sbjct: 11  AAAFFAIVFVVLVGVARSE-ECTRTCIAQNCDTLSIRYGKYCGIGHSGCPGEEPCDDLDA 70

Query: 75  CCKDHDECVEYKGLTNIKCHIKFKDCITKL------QKSGMAGFSQQCPYSIAIPTMVNG 134
           CCK HD CVE  G+TNI CH KF+ C+ +L       K+   GFS +CPYS+ IPT+  G
Sbjct: 71  CCKIHDHCVELNGMTNISCHKKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQG 130

Query: 135 MDIAIMFSQFSS 141
           MDI I+FSQ  +
Sbjct: 131 MDIGILFSQLGN 141

[Close]

BLAST of Carg10813-RA vs. TAIR 10
Match: AT2G19690.2 (phospholipase A2-beta )

HSP 1 Score: 150.6 bits (379), Expect = 9.7e-37
Identity = 67/131 (51.15%), Postives = 92/131 (70.23%), Query Frame = 0


Query: 15  SAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDA 74
           +A F  +V + +V    S+ EC+RTC ++ C+T+ IRYGK+CG+G +GC GE+PCD+LDA
Sbjct: 11  AAAFFAIVFVVLVGVARSE-ECTRTCIAQNCDTLSIRYGKYCGIGHSGCPGEEPCDDLDA 70

Query: 75  CCKDHDECVEYKGLTNIKCHIKFKDCITKL------QKSGMAGFSQQCPYSIAIPTMVNG 134
           CCK HD CVE  G+TNI CH KF+ C+ +L       K+   GFS +CPYS+ IPT+  G
Sbjct: 71  CCKIHDHCVELNGMTNISCHKKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQG 130

Query: 135 MDIAIMFSQFS 140
           MDI I+FSQ +
Sbjct: 131 MDIGILFSQLA 140

[Close]

BLAST of Carg10813-RA vs. TAIR 10
Match: AT4G29460.1 (Phospholipase A2 family protein )

HSP 1 Score: 150.6 bits (379), Expect = 9.7e-37
Identity = 69/121 (57.02%), Postives = 85/121 (70.25%), Query Frame = 0


Query: 22  VLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKPCDELDACCKDHDE 81
           +LL VV   SSQ +CS TC ++ CN++GIRYGK+CG+G+ GC GE PCD+LDACC  HD 
Sbjct: 18  LLLAVV---SSQEKCSNTCIAQNCNSLGIRYGKYCGIGYFGCPGEPPCDDLDACCMTHDN 77

Query: 82  CVEYKGLTNIKCHIKFKDCITKLQKS------GMAGFSQQCPYSIAIPTMVNGMDIAIMF 137
           CV+ KG+T + CH +FK C+ KL KS         GFS QCPYSI IPT+ NGMD  I F
Sbjct: 78  CVDLKGMTYVNCHKQFKRCVNKLSKSIKHSNGEKIGFSTQCPYSIVIPTVFNGMDYGIFF 135

[Close]

BLAST of Carg10813-RA vs. TAIR 10
Match: AT4G29470.1 (Phospholipase A2 family protein )

HSP 1 Score: 137.5 bits (345), Expect = 8.5e-33
Identity = 60/134 (44.78%), Postives = 89/134 (66.42%), Query Frame = 0


Query: 9   LRKQVASAIFLLLVLLTVVSECSSQVECSRTCASEKCNTVGIRYGKFCGVGWTGCAGEKP 68
           +R    + + L L +  +++   SQ +CS+TC ++KCN +GIRYGK+CG+G+ GC GE P
Sbjct: 2   IRGGALTHVALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLGIRYGKYCGIGYFGCPGEPP 61

Query: 69  CDELDACCKDHDECVEYKGLTNIKCHIKFKDCITKLQKS------GMAGFSQQCPYSIAI 128
           CD+LD CC  HD CV+ KG+T + CH +F+ C+ +L++S         GFS++CPYS  I
Sbjct: 62  CDDLDDCCMTHDNCVDLKGMTYVDCHKQFQRCVNELKQSIQESNNQKVGFSKECPYSTVI 121

Query: 129 PTMVNGMDIAIMFS 137
           PT+  GM+  I FS
Sbjct: 122 PTVYRGMNYGIFFS 135

[Close]

BLAST of Carg10813-RA vs. TAIR 10
Match: AT2G06925.1 (Phospholipase A2 family protein )

HSP 1 Score: 77.4 bits (189), Expect = 1.0e-14
Identity = 39/104 (37.50%), Postives = 58/104 (55.77%), Query Frame = 0


Query: 35  ECSRTCASEKCNTVG-IRYGKFCGVGWTGCAGEKPCDELDACCKDHDECVEYKG--LTNI 94
           ECSR C SE C+    +RYGK+CG+ ++GC GE+PCD LD+CC  HD CV+ K     + 
Sbjct: 37  ECSRKCESEFCSVPPFLRYGKYCGLLYSGCPGERPCDGLDSCCMKHDACVQSKNNDYLSQ 96

Query: 95  KCHIKFKDCITKLQKSGMAGF-SQQCPYSIAIPTMVNGMDIAIM 135
           +C  KF +C+    +     F   +C     I  +   M+ A++
Sbjct: 97  ECSQKFINCMNNFSQKKQPTFKGNKCDADEVIDVISIVMEAALI 140

The following BLAST results are available for this feature:

BLAST of Carg10813-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7030479.1	2.8e-78	100.00	putative phospholipase A2-like 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022946468.1	6.2e-78	99.31	probable phospholipase A2 homolog 1 [Cucurbita moschata]	[more]
XP_022999541.1	2.0e-76	97.24	probable phospholipase A2 homolog 1 [Cucurbita maxima]	[more]
XP_023545532.1	2.2e-75	96.55	probable phospholipase A2 homolog 1 [Cucurbita pepo subsp. pepo]	[more]
KAG6599502.1	8.5e-67	100.00	60S ribosomal protein L28-2, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Carg10813-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XG80	8.3e-41	61.82	Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
Q8GZB4	1.4e-35	50.76	Phospholipase A2-beta OS=Arabidopsis thaliana OX=3702 GN=PLA2-BETA PE=1 SV=1	[more]
Q9M0D7	1.4e-35	57.02	Phospholipase A2-gamma OS=Arabidopsis thaliana OX=3702 GN=PLA2-GAMMA PE=1 SV=1	[more]
Q8GV50	1.2e-31	44.78	Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1	[more]
Q10E50	7.1e-16	43.37	Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III ...	[more]

BLAST of Carg10813-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G3Y1	3.0e-78	99.31	probable phospholipase A2 homolog 1 OS=Cucurbita moschata OX=3662 GN=LOC11145051...	[more]
A0A6J1KDD2	9.7e-77	97.24	probable phospholipase A2 homolog 1 OS=Cucurbita maxima OX=3661 GN=LOC111493870 ...	[more]
A0A6J1DMI5	8.9e-54	70.27	probable phospholipase A2 homolog 1 OS=Momordica charantia OX=3673 GN=LOC1110219...	[more]
A0A1S3C986	1.2e-53	75.94	probable phospholipase A2 homolog 1 OS=Cucumis melo OX=3656 GN=LOC103498425 PE=4...	[more]
A0A0A0LFR9	6.3e-52	74.44	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000560 PE=4 SV=1	[more]

BLAST of Carg10813-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G19690.1	9.7e-37	50.76	phospholipase A2-beta	[more]
AT2G19690.2	9.7e-37	51.15	phospholipase A2-beta	[more]
AT4G29460.1	9.7e-37	57.02	Phospholipase A2 family protein	[more]
AT4G29470.1	8.5e-33	44.78	Phospholipase A2 family protein	[more]
AT2G06925.1	1.0e-14	37.50	Phospholipase A2 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036444	Phospholipase A2 domain superfamily	GENE3D	1.20.90.10	Phospholipase A2 domain	coord: 22..142 e-value: 7.4E-31 score: 108.5
IPR036444	Phospholipase A2 domain superfamily	SUPERFAMILY	48619	Phospholipase A2, PLA2	coord: 33..137
None	No IPR available	PANTHER	PTHR11716:SF51	PHOSPHOLIPASE A(2)	coord: 20..130
IPR001211	Phospholipase A2	PANTHER	PTHR11716	PHOSPHOLIPASE A2 FAMILY MEMBER	coord: 20..130
IPR033113	Phospholipase A2, histidine active site	PROSITE	PS00118	PA2_HIS	coord: 75..82

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg10813	Carg10813	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg10813-RA:4	Carg10813-RA:4	exon
Carg10813-RA:3	Carg10813-RA:3	exon
Carg10813-RA:2	Carg10813-RA:2	exon
Carg10813-RA:1	Carg10813-RA:1	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg10813-RA:three_prime_utr	Carg10813-RA:three_prime_utr	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg10813-RA:cds	Carg10813-RA:cds_4	CDS
Carg10813-RA:cds	Carg10813-RA:cds_3	CDS
Carg10813-RA:cds	Carg10813-RA:cds_2	CDS
Carg10813-RA:cds	Carg10813-RA:cds	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg10813-RA:five_prime_utr	Carg10813-RA:five_prime_utr	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg10813-RA	Carg10813-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050482	arachidonic acid secretion
biological_process	GO:0016042	lipid catabolic process
biological_process	GO:0006644	phospholipid metabolic process
biological_process	GO:0009555	pollen development
biological_process	GO:0009846	pollen germination
biological_process	GO:0009860	pollen tube growth
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016020	membrane
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0004623	phospholipase A2 activity
molecular_function	GO:0047498	calcium-dependent phospholipase A2 activity
molecular_function	GO:0005543	phospholipid binding