Carg05816-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05816-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Tetratricopeptide-like helical
Location	Carg_Chr16: 4634976 .. 4635371 (-)
Sequence length	396
RNA-Seq Expression	Carg05816-RA
Synteny	Carg05816-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGTTTCTTTGGAAGCTTTGGCAATGGCGGGGATTACTTCCAATGAGTGGGCTATGGATGCTGAAGAATGGGAACGAAACGAAATCGAGAACGACGTTCCACCGCATTTATTAGCCGATGAAGACGACGATGACGATAACGTAACGAGTACTGTCAGCAATTATAGCTTCCCGGTCGCTGGATTCTTCGATAGCTTGCATCGGGAAGATGGCGGAGCCGACGATATCCATTGCTTCTGGGGGATCTTGTGTAGCTTAAGCAAAAGCTTAGAGAAGAGGGCTTCTAATGGGATGAGTTCAGTGAGAATCATTGTGAGAACTATTTTAGGGTTTCTACTGGTGAAGATCTTGAAGATCATTAGTGCACACACATCCAACATGATTATTCCTTGA

mRNA sequence

ATGTCGGTTTCTTTGGAAGCTTTGGCAATGGCGGGGATTACTTCCAATGAGTGGGCTATGGATGCTGAAGAATGGGAACGAAACGAAATCGAGAACGACGTTCCACCGCATTTATTAGCCGATGAAGACGACGATGACGATAACGTAACGAGTACTGTCAGCAATTATAGCTTCCCGGTCGCTGGATTCTTCGATAGCTTGCATCGGGAAGATGGCGGAGCCGACGATATCCATTGCTTCTGGGGGATCTTGTGTAGCTTAAGCAAAAGCTTAGAGAAGAGGGCTTCTAATGGGATGAGTTCAGTGAGAATCATTGTGAGAACTATTTTAGGGTTTCTACTGGTGAAGATCTTGAAGATCATTAGTGCACACACATCCAACATGATTATTCCTTGA

Coding sequence (CDS)

ATGTCGGTTTCTTTGGAAGCTTTGGCAATGGCGGGGATTACTTCCAATGAGTGGGCTATGGATGCTGAAGAATGGGAACGAAACGAAATCGAGAACGACGTTCCACCGCATTTATTAGCCGATGAAGACGACGATGACGATAACGTAACGAGTACTGTCAGCAATTATAGCTTCCCGGTCGCTGGATTCTTCGATAGCTTGCATCGGGAAGATGGCGGAGCCGACGATATCCATTGCTTCTGGGGGATCTTGTGTAGCTTAAGCAAAAGCTTAGAGAAGAGGGCTTCTAATGGGATGAGTTCAGTGAGAATCATTGTGAGAACTATTTTAGGGTTTCTACTGGTGAAGATCTTGAAGATCATTAGTGCACACACATCCAACATGATTATTCCTTGA

Protein sequence

MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDNVTSTVSNYSFPVAGFFDSLHREDGGADDIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLLVKILKIISAHTSNMIIP

Homology

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BLAST of Carg05816-RA vs. NCBI nr
Match: KAG7015383.1 (hypothetical protein SDJN02_23018, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 259.6 bits (662), Expect = 1.4e-65
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDNVTSTVSNYSFPV 60
           MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDNVTSTVSNYSFPV
Sbjct: 1   MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDNVTSTVSNYSFPV 60

Query: 61  AGFFDSLHREDGGADDIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLLVKILKI 120
           AGFFDSLHREDGGADDIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLLVKILKI
Sbjct: 61  AGFFDSLHREDGGADDIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLLVKILKI 120

Query: 121 ISAHTSNMIIP 132
           ISAHTSNMIIP
Sbjct: 121 ISAHTSNMIIP 131

[Close]

BLAST of Carg05816-RA vs. NCBI nr
Match: KAG6577295.1 (hypothetical protein SDJN03_24869, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 253.8 bits (647), Expect = 7.9e-64
Identity = 129/131 (98.47%), Postives = 129/131 (98.47%), Query Frame = 0


Query: 1   MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDNVTSTVSNYSFPV 60
           MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDNVTSTVSNYSFPV
Sbjct: 1   MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDNVTSTVSNYSFPV 60

Query: 61  AGFFDSLHREDGGADDIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLLVKILKI 120
           AGFFDSL REDGGA DIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLLVKILKI
Sbjct: 61  AGFFDSLRREDGGAHDIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLLVKILKI 120

Query: 121 ISAHTSNMIIP 132
           ISAHTSNMIIP
Sbjct: 121 ISAHTSNMIIP 131

[Close]

BLAST of Carg05816-RA vs. NCBI nr
Match: TYK16880.1 (Tetratricopeptide-like helical [Cucumis melo var. makuwa])

HSP 1 Score: 154.5 bits (389), Expect = 6.5e-34
Identity = 89/130 (68.46%), Postives = 98/130 (75.38%), Query Frame = 0


Query: 1   MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDN--VTSTVSNYSF 60
           MSVSLEALAMAGI SNEW MDA+EWERNEI+  VPPHLLADE+DD+D   VTST+SNY F
Sbjct: 1   MSVSLEALAMAGIDSNEWGMDAQEWERNEID-VVPPHLLADEEDDEDGEYVTSTISNYCF 60

Query: 61  PVAGFFDSLHREDGGADDIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLLVKIL 120
            V G F    R D  AD      GI  SLSK +EKRASNGM+SVRIIVRTILGFL+V  +
Sbjct: 61  RVDGSFGGWRRRDWKADGALRILGIFYSLSKVIEKRASNGMNSVRIIVRTILGFLMVMTM 120

Query: 121 KIISAHTSNM 129
           KIISA  S M
Sbjct: 121 KIISAQRSKM 129

[Close]

BLAST of Carg05816-RA vs. NCBI nr
Match: KAA0044707.1 (Tetratricopeptide-like helical [Cucumis melo var. makuwa])

HSP 1 Score: 148.3 bits (373), Expect = 4.7e-32
Identity = 83/117 (70.94%), Postives = 91/117 (77.78%), Query Frame = 0


Query: 1   MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDN--VTSTVSNYSF 60
           MSVSLEALAMAGI SNEW MDA+EWERNEI+  VPPHLLADE+DD+D   VTST+SNY F
Sbjct: 1   MSVSLEALAMAGIDSNEWGMDAQEWERNEID-VVPPHLLADEEDDEDGEYVTSTISNYCF 60

Query: 61  PVAGFFDSLHREDGGADDIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLLV 116
            V G F    R D  AD  H   GI  SLSK +EKRASNGM+SVRIIVRTILGFL+V
Sbjct: 61  RVDGSFGGWRRRDWKADGAHRILGIFYSLSKVIEKRASNGMNSVRIIVRTILGFLMV 116

[Close]

BLAST of Carg05816-RA vs. NCBI nr
Match: XP_022943015.1 (uncharacterized protein LOC111447880 [Cucurbita moschata])

HSP 1 Score: 116.3 bits (290), Expect = 2.0e-22
Identity = 65/126 (51.59%), Postives = 84/126 (66.67%), Query Frame = 0


Query: 1   MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDNVTSTVSNYSFPV 60
           MSVSLEALAMAGI SNEWAMDAEEWERNE+E D+PPHLLADEDD++D +++      FP 
Sbjct: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEMEKDIPPHLLADEDDEEDEMST-----KFPA 60

Query: 61  AGFFDSLHREDGGADDIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLLVKILKI 120
                S  R+D G   + C   I+  L K  EKR  N +SS+ I+   ++GFL+   +KI
Sbjct: 61  TRC--SCCRKDDGVGGVSCSPTIMWHLRKITEKRDLNCLSSMEIVTSAVVGFLMFMTIKI 119

Query: 121 ISAHTS 127
           I++  S
Sbjct: 121 IASKRS 119

[Close]

BLAST of Carg05816-RA vs. ExPASy TrEMBL
Match: A0A0A0KWE7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G026810 PE=4 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 7.5e-36
Identity = 93/134 (69.40%), Postives = 101/134 (75.37%), Query Frame = 0


Query: 1   MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDN----VTSTVSNY 60
           MSVSLEALAMAGI SNEWAMDAEEWERNEI+  VPPHLLADE+DD+D      TST+SNY
Sbjct: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEID-VVPPHLLADEEDDEDGDEHVTTSTISNY 60

Query: 61  SFPVAGFFDSLHREDGGAD--DIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLL 120
            FPV G F+     D  AD    H   GI CSLSK +EKRASNGMSSVRIIVRTILGFL+
Sbjct: 61  CFPVDGSFEGWRGRDCKADGGHGHGILGIFCSLSKVIEKRASNGMSSVRIIVRTILGFLM 120

Query: 121 VKILKIISAHTSNM 129
           V  +KIISA  S M
Sbjct: 121 VMTMKIISAQRSKM 133

[Close]

BLAST of Carg05816-RA vs. ExPASy TrEMBL
Match: A0A5D3CZU0 (Tetratricopeptide-like helical OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00110 PE=4 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 3.2e-34
Identity = 89/130 (68.46%), Postives = 98/130 (75.38%), Query Frame = 0


Query: 1   MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDN--VTSTVSNYSF 60
           MSVSLEALAMAGI SNEW MDA+EWERNEI+  VPPHLLADE+DD+D   VTST+SNY F
Sbjct: 1   MSVSLEALAMAGIDSNEWGMDAQEWERNEID-VVPPHLLADEEDDEDGEYVTSTISNYCF 60

Query: 61  PVAGFFDSLHREDGGADDIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLLVKIL 120
            V G F    R D  AD      GI  SLSK +EKRASNGM+SVRIIVRTILGFL+V  +
Sbjct: 61  RVDGSFGGWRRRDWKADGALRILGIFYSLSKVIEKRASNGMNSVRIIVRTILGFLMVMTM 120

Query: 121 KIISAHTSNM 129
           KIISA  S M
Sbjct: 121 KIISAQRSKM 129

[Close]

BLAST of Carg05816-RA vs. ExPASy TrEMBL
Match: A0A5A7TS68 (Tetratricopeptide-like helical OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004640 PE=4 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.3e-32
Identity = 83/117 (70.94%), Postives = 91/117 (77.78%), Query Frame = 0


Query: 1   MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDN--VTSTVSNYSF 60
           MSVSLEALAMAGI SNEW MDA+EWERNEI+  VPPHLLADE+DD+D   VTST+SNY F
Sbjct: 1   MSVSLEALAMAGIDSNEWGMDAQEWERNEID-VVPPHLLADEEDDEDGEYVTSTISNYCF 60

Query: 61  PVAGFFDSLHREDGGADDIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLLV 116
            V G F    R D  AD  H   GI  SLSK +EKRASNGM+SVRIIVRTILGFL+V
Sbjct: 61  RVDGSFGGWRRRDWKADGAHRILGIFYSLSKVIEKRASNGMNSVRIIVRTILGFLMV 116

[Close]

BLAST of Carg05816-RA vs. ExPASy TrEMBL
Match: A0A6J1FX98 (uncharacterized protein LOC111447880 OS=Cucurbita moschata OX=3662 GN=LOC111447880 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 9.5e-23
Identity = 65/126 (51.59%), Postives = 84/126 (66.67%), Query Frame = 0


Query: 1   MSVSLEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLADEDDDDDNVTSTVSNYSFPV 60
           MSVSLEALAMAGI SNEWAMDAEEWERNE+E D+PPHLLADEDD++D +++      FP 
Sbjct: 1   MSVSLEALAMAGIDSNEWAMDAEEWERNEMEKDIPPHLLADEDDEEDEMST-----KFPA 60

Query: 61  AGFFDSLHREDGGADDIHCFWGILCSLSKSLEKRASNGMSSVRIIVRTILGFLLVKILKI 120
                S  R+D G   + C   I+  L K  EKR  N +SS+ I+   ++GFL+   +KI
Sbjct: 61  TRC--SCCRKDDGVGGVSCSPTIMWHLRKITEKRDLNCLSSMEIVTSAVVGFLMFMTIKI 119

Query: 121 ISAHTS 127
           I++  S
Sbjct: 121 IASKRS 119

[Close]

BLAST of Carg05816-RA vs. ExPASy TrEMBL
Match: A0A0A0KU90 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G026820 PE=4 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 6.4e-11
Identity = 49/100 (49.00%), Postives = 62/100 (62.00%), Query Frame = 0


Query: 1  MSVSLEALAMAGITSNEWAMDAEEWERN--EIEND--VPPHLLADEDDDDDNVTSTVSNY 60
          M VSLEALAMAGI SNEWAMDAEEW+ N  ++END  +PPHLLADED+++      ++  
Sbjct: 1  MRVSLEALAMAGIDSNEWAMDAEEWKTNTHQMENDDIIPPHLLADEDEEN------IAAK 60

Query: 61 SFPVAGFFDSLHREDGGADDIHCFWGILCSLSKSLEKRAS 97
             ++G     HR+D G      F      L K +EKRAS
Sbjct: 61 ELCISG-----HRKDDGLVVFCAFRRSYAILRKMMEKRAS 89

[Close]

BLAST of Carg05816-RA vs. TAIR 10
Match: AT2G22320.1 (unknown protein; Has 7 Blast hits to 7 proteins in 4 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 7; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 41.6 bits (96), Expect = 5.7e-04
Identity = 26/57 (45.61%), Postives = 32/57 (56.14%), Query Frame = 0


Query: 5  LEALAMAGITSNEWAMDAEEWERNEIENDVPPHLLAD------------EDDDDDNV 50
          +EALAMAG+   EW +  EEWE    E+ VP HLLAD            ++DDDD V
Sbjct: 1  MEALAMAGVDYQEWGLSIEEWELQ--ESVVPLHLLADDFKEEEQEEDHQDNDDDDRV 55

The following BLAST results are available for this feature:

BLAST of Carg05816-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7015383.1	1.4e-65	100.00	hypothetical protein SDJN02_23018, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6577295.1	7.9e-64	98.47	hypothetical protein SDJN03_24869, partial [Cucurbita argyrosperma subsp. sorori...	[more]
TYK16880.1	6.5e-34	68.46	Tetratricopeptide-like helical [Cucumis melo var. makuwa]	[more]
KAA0044707.1	4.7e-32	70.94	Tetratricopeptide-like helical [Cucumis melo var. makuwa]	[more]
XP_022943015.1	2.0e-22	51.59	uncharacterized protein LOC111447880 [Cucurbita moschata]	[more]

BLAST of Carg05816-RA vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg05816-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KWE7	7.5e-36	69.40	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G026810 PE=4 SV=1	[more]
A0A5D3CZU0	3.2e-34	68.46	Tetratricopeptide-like helical OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A5A7TS68	2.3e-32	70.94	Tetratricopeptide-like helical OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s...	[more]
A0A6J1FX98	9.5e-23	51.59	uncharacterized protein LOC111447880 OS=Cucurbita moschata OX=3662 GN=LOC1114478...	[more]
A0A0A0KU90	6.4e-11	49.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G026820 PE=4 SV=1	[more]

BLAST of Carg05816-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G22320.1	5.7e-04	45.61	unknown protein; Has 7 Blast hits to 7 proteins in 4 species: Archae - 0; Bacter...	[more]

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg05816	Carg05816	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg05816-RA:1	Carg05816-RA:1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg05816-RA:cds	Carg05816-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg05816-RA	Carg05816-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane