Carg05144-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05144-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Protein kinase domain-containing protein
Location	Carg_Chr07: 3138823 .. 3140205 (-)
Sequence length	891
RNA-Seq Expression	Carg05144-RA
Synteny	Carg05144-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCAGCTGATAATTCAATTCCATTTGACCGAGATGCCGAGCCTTTTGTTGAGGTCGATCCAACTGGAAGGTTTGGACGATACGATGATCTTCTTGGGTCTGGAGCAGTGAAGAAAGTATATCGGGCATTTGATCAGGAGGAAGGTATAGAGGTTGCTTGGAATCAGGTTCGATTGAGGAAATTCAGTGGGGATCCAGTGTTCATCAACCGCTTGAGATCGGAGGTTCAGCTGCTGAGCACGTTGAGTAACGATTATATCATAGTTTGCTACAGTGTTTGGAATGATGATGAGCACAATACGTTGAATTTCATTACCGAGGTGTGTACATCAGGAAATCTGAGGGATTATCGTAAGAAACATCGACATGTGTCAACTAAGGCCTTGAAGAAGTGGTCAAAGCAGGTACTTGAGGGATTGGATTATCTTCATAATCATGAGCCTTGCATTATTCACCGAGATCTCAACTGCAGTAACATATTTGTCAATGGGAATATTGGTCAGGTATTTGCTTCACTCTTGATAATGCTACTATTTCTAACCTAAGATAAATTTTGCAGATGACTTTTCTTTTTTTCGGTTTTTATATTCTTGATCATTATTTTAGGTGCAAGCAAATCACCTTTTGTGGAAATTCAAAAAAAATGCCGATGACTCATTAAAAGATTTGCCTGAGCTGCTTGAATAATAGTCCATATGCTACACATGATTGTCGATTGACTTTGTACTGATTGAAGCTAAAACTGAGGTTATGATCAATTAAAAGATTTGTCGATTGGCTTTGTCAAATCTCCCTTGTTCACAAGAACGTGCATGTGGTTAGACAAATTCATTTTAGAACATTGTTTCCTGAATCCAAGTATCAACTATATAATCTCAAATTTGCCTGCGAATCTATATGATTCCCAAAATAAGATTAAACCACAAGTATCCAATAGTACATCCACTTGTTTATCTTTGTGGTGTTTTGAAGCGTGATTACACTCATGTTTACAGGTGAAAATAGGTGATCTAGGTTTTGCAGCTATAGTTGGCAAGAGTCATGCTGCGCACTCAATCATAGGCACACCGGAGTATATGGCACCAGAACTGTACGAGGAAGATTACACAGAGATGGTGGATATATACTCTTTTGCGATGTGTTTGCTTGAGATGGTCACGATGGAGATACCATACAGTGAATGTGATAGTATTGCCAAGATATACAAGAAGGTGACGACTGGAATAAAACCCCAAGCATTAAACAAAGTGACAAATGATGAAGTCAGGGCTTTCATTGAGAAGTGCATTGCACAACCAAGGGCAAGGCCTACCACTTCTGAACTTCTCAAGGACCCCTTCTTCGATGAAGTGAGGGATGAAGATTCCGAACAGAGTTATTAG

mRNA sequence

ATGCCAGCTGATAATTCAATTCCATTTGACCGAGATGCCGAGCCTTTTGTTGAGGTCGATCCAACTGGAAGGTTTGGACGATACGATGATCTTCTTGGGTCTGGAGCAGTGAAGAAAGTATATCGGGCATTTGATCAGGAGGAAGGTATAGAGGTTGCTTGGAATCAGGTTCGATTGAGGAAATTCAGTGGGGATCCAGTGTTCATCAACCGCTTGAGATCGGAGGTTCAGCTGCTGAGCACGTTGAGTAACGATTATATCATAGTTTGCTACAGTGTTTGGAATGATGATGAGCACAATACGTTGAATTTCATTACCGAGGTGTGTACATCAGGAAATCTGAGGGATTATCGTAAGAAACATCGACATGTGTCAACTAAGGCCTTGAAGAAGTGGTCAAAGCAGGTACTTGAGGGATTGGATTATCTTCATAATCATGAGCCTTGCATTATTCACCGAGATCTCAACTGCAGTAACATATTTGTCAATGGGAATATTGGTCAGGTGAAAATAGGTGATCTAGGTTTTGCAGCTATAGTTGGCAAGAGTCATGCTGCGCACTCAATCATAGGCACACCGGAGTATATGGCACCAGAACTGTACGAGGAAGATTACACAGAGATGGTGGATATATACTCTTTTGCGATGTGTTTGCTTGAGATGGTCACGATGGAGATACCATACAGTGAATGTGATAGTATTGCCAAGATATACAAGAAGGTGACGACTGGAATAAAACCCCAAGCATTAAACAAAGTGACAAATGATGAAGTCAGGGCTTTCATTGAGAAGTGCATTGCACAACCAAGGGCAAGGCCTACCACTTCTGAACTTCTCAAGGACCCCTTCTTCGATGAAGTGAGGGATGAAGATTCCGAACAGAGTTATTAG

Coding sequence (CDS)

ATGCCAGCTGATAATTCAATTCCATTTGACCGAGATGCCGAGCCTTTTGTTGAGGTCGATCCAACTGGAAGGTTTGGACGATACGATGATCTTCTTGGGTCTGGAGCAGTGAAGAAAGTATATCGGGCATTTGATCAGGAGGAAGGTATAGAGGTTGCTTGGAATCAGGTTCGATTGAGGAAATTCAGTGGGGATCCAGTGTTCATCAACCGCTTGAGATCGGAGGTTCAGCTGCTGAGCACGTTGAGTAACGATTATATCATAGTTTGCTACAGTGTTTGGAATGATGATGAGCACAATACGTTGAATTTCATTACCGAGGTGTGTACATCAGGAAATCTGAGGGATTATCGTAAGAAACATCGACATGTGTCAACTAAGGCCTTGAAGAAGTGGTCAAAGCAGGTACTTGAGGGATTGGATTATCTTCATAATCATGAGCCTTGCATTATTCACCGAGATCTCAACTGCAGTAACATATTTGTCAATGGGAATATTGGTCAGGTGAAAATAGGTGATCTAGGTTTTGCAGCTATAGTTGGCAAGAGTCATGCTGCGCACTCAATCATAGGCACACCGGAGTATATGGCACCAGAACTGTACGAGGAAGATTACACAGAGATGGTGGATATATACTCTTTTGCGATGTGTTTGCTTGAGATGGTCACGATGGAGATACCATACAGTGAATGTGATAGTATTGCCAAGATATACAAGAAGGTGACGACTGGAATAAAACCCCAAGCATTAAACAAAGTGACAAATGATGAAGTCAGGGCTTTCATTGAGAAGTGCATTGCACAACCAAGGGCAAGGCCTACCACTTCTGAACTTCTCAAGGACCCCTTCTTCGATGAAGTGAGGGATGAAGATTCCGAACAGAGTTATTAG

Protein sequence

MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLRKFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKKHRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIVGKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKKVTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY

Homology

[Close]

BLAST of Carg05144-RA vs. NCBI nr
Match: KAG7026985.1 (putative serine/threonine-protein kinase WNK11, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 607.1 bits (1564), Expect = 8.3e-170
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0


Query: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60
           MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR
Sbjct: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60

Query: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120
           KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK
Sbjct: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120

Query: 121 HRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180
           HRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV
Sbjct: 121 HRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180

Query: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240
           GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK
Sbjct: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240

Query: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY 297
           VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY
Sbjct: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY 296

[Close]

BLAST of Carg05144-RA vs. NCBI nr
Match: XP_022946942.1 (probable serine/threonine-protein kinase WNK11 [Cucurbita moschata] >XP_022946943.1 probable serine/threonine-protein kinase WNK11 [Cucurbita moschata] >XP_023532547.1 probable serine/threonine-protein kinase WNK11 [Cucurbita pepo subsp. pepo] >XP_023532548.1 probable serine/threonine-protein kinase WNK11 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 604.7 bits (1558), Expect = 4.1e-169
Identity = 295/296 (99.66%), Postives = 295/296 (99.66%), Query Frame = 0


Query: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60
           MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR
Sbjct: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60

Query: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120
           KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK
Sbjct: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120

Query: 121 HRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180
           HRHVS KALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV
Sbjct: 121 HRHVSIKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180

Query: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240
           GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK
Sbjct: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240

Query: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY 297
           VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY
Sbjct: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY 296

[Close]

BLAST of Carg05144-RA vs. NCBI nr
Match: XP_022971060.1 (probable serine/threonine-protein kinase WNK11 [Cucurbita maxima] >XP_022971061.1 probable serine/threonine-protein kinase WNK11 [Cucurbita maxima] >XP_022971062.1 probable serine/threonine-protein kinase WNK11 [Cucurbita maxima])

HSP 1 Score: 601.3 bits (1549), Expect = 4.6e-168
Identity = 294/296 (99.32%), Postives = 294/296 (99.32%), Query Frame = 0


Query: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60
           MPADNSIPFDRDAEPFVEVDPTGRFGRYD LLGSGAVKKVYRAFDQEEGIEVAWNQVRLR
Sbjct: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDVLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60

Query: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120
           KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK
Sbjct: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120

Query: 121 HRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180
           HRHVS KALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV
Sbjct: 121 HRHVSIKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180

Query: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240
           GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK
Sbjct: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240

Query: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY 297
           VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY
Sbjct: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY 296

[Close]

BLAST of Carg05144-RA vs. NCBI nr
Match: KAG6604902.1 (putative serine/threonine-protein kinase WNK11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 586.6 bits (1511), Expect = 1.2e-163
Identity = 285/286 (99.65%), Postives = 285/286 (99.65%), Query Frame = 0


Query: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60
           MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLG GAVKKVYRAFDQEEGIEVAWNQVRLR
Sbjct: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGCGAVKKVYRAFDQEEGIEVAWNQVRLR 60

Query: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120
           KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK
Sbjct: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120

Query: 121 HRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180
           HRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV
Sbjct: 121 HRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180

Query: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240
           GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK
Sbjct: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240

Query: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDE 287
           VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDE
Sbjct: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDE 286

[Close]

BLAST of Carg05144-RA vs. NCBI nr
Match: XP_008464187.1 (PREDICTED: probable serine/threonine-protein kinase WNK11 isoform X1 [Cucumis melo] >XP_008464188.1 PREDICTED: probable serine/threonine-protein kinase WNK11 isoform X1 [Cucumis melo])

HSP 1 Score: 579.7 bits (1493), Expect = 1.4e-161
Identity = 280/295 (94.92%), Postives = 289/295 (97.97%), Query Frame = 0


Query: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60
           MPA+NSIP+DRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR
Sbjct: 2   MPAENSIPYDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 61

Query: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120
            FSGDPVFINRLRSEVQLLSTL+N YIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK
Sbjct: 62  NFSGDPVFINRLRSEVQLLSTLNNKYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 121

Query: 121 HRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180
           HRHVS KALKKWSKQVLEGLDYLH HEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV
Sbjct: 122 HRHVSIKALKKWSKQVLEGLDYLHTHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 181

Query: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240
           G+SHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDS+AKIYKK
Sbjct: 182 GRSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSVAKIYKK 241

Query: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQS 296
           VTTGIKPQA+ KVT+DEVRAFIEKCIAQPRARP+ SELLKDPFFDEVRDEDSEQ+
Sbjct: 242 VTTGIKPQAITKVTDDEVRAFIEKCIAQPRARPSASELLKDPFFDEVRDEDSEQT 296

[Close]

BLAST of Carg05144-RA vs. ExPASy Swiss-Prot
Match: Q6ICW6 (Probable serine/threonine-protein kinase WNK11 OS=Arabidopsis thaliana OX=3702 GN=WNK11 PE=2 SV=1)

HSP 1 Score: 467.2 bits (1201), Expect = 1.3e-130
Identity = 219/290 (75.52%), Postives = 253/290 (87.24%), Query Frame = 0


Query: 4   DNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLRKFS 63
           DN    D+D+E FVEVDPTGR+GRY +LLGSGAVKKVYRAFDQEEGIEVAWNQV+LR FS
Sbjct: 9   DNESEKDKDSESFVEVDPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRCFS 68

Query: 64  GDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKKHRH 123
            DP    RL SEV+LL  L N  II  Y VW D+ +NTLNFITE+CTSGNLR+YRKKHRH
Sbjct: 69  DDPAMTERLYSEVRLLKNLKNSNIITLYKVWRDERNNTLNFITEICTSGNLREYRKKHRH 128

Query: 124 VSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIVGKS 183
           VS +ALKKWSKQ+L+GLDYLH H+PCIIHRDLNCSNIFVNGNIGQVKIGDLG AAIVGK+
Sbjct: 129 VSMRALKKWSKQILKGLDYLHTHDPCIIHRDLNCSNIFVNGNIGQVKIGDLGLAAIVGKN 188

Query: 184 HAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKKVTT 243
           H AHSI+GTPE+MAPELYEE+YTEMVDIYS+ MC+LE+V++EIPYSECDS+AKIYK+V+ 
Sbjct: 189 HLAHSILGTPEFMAPELYEENYTEMVDIYSYGMCVLELVSLEIPYSECDSVAKIYKRVSK 248

Query: 244 GIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSE 294
           G+KP+ALNKV + E +AFIEKCIAQPRARP+ +ELL DPFFD + D+D E
Sbjct: 249 GLKPEALNKVNDPEAKAFIEKCIAQPRARPSAAELLCDPFFDGILDDDDE 298

[Close]

BLAST of Carg05144-RA vs. ExPASy Swiss-Prot
Match: Q0D541 (Probable serine/threonine-protein kinase WNK5 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK5 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.0e-109
Identity = 185/282 (65.60%), Postives = 230/282 (81.56%), Query Frame = 0


Query: 12  DAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLRKFSG-DPVFIN 71
           + E F EVDPTGRFGRY D+LG G+VKKVYR FDQEEGIEVAWN+VRLR  +  DP  + 
Sbjct: 41  EEEAFEEVDPTGRFGRYADVLGLGSVKKVYRGFDQEEGIEVAWNRVRLRALADRDPAMVE 100

Query: 72  RLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKKHRHVSTKALK 131
           RL +EV+LL +L +++II  + VW D +   LNFITEVCTSG+LR+YR +HRHVS KALK
Sbjct: 101 RLHAEVRLLRSLHHEHIIGFHKVWLDRDAGVLNFITEVCTSGSLREYRDRHRHVSVKALK 160

Query: 132 KWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIVGKSHAAHSII 191
           KW++Q+L GLD+LH H+PCIIHRDLNCSN+F+NGN GQVKIGDLG AAIV K+H AH+I+
Sbjct: 161 KWARQILLGLDHLHTHDPCIIHRDLNCSNVFINGNTGQVKIGDLGLAAIVDKTHVAHTIL 220

Query: 192 GTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKKVTTGIKPQAL 251
           GTPE+MAPELY E YTE VDIYS+ MC+LEMVT E+PY+ECDS+ +IY  VT G+ P AL
Sbjct: 221 GTPEFMAPELYTETYTESVDIYSYGMCVLEMVTREMPYAECDSVVQIYHSVTRGVPPAAL 280

Query: 252 NKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDS 293
            ++ + E+RAFIE+CI QPR RP+ +ELL+DPFF  + D+DS
Sbjct: 281 KRIRDPELRAFIERCIGQPRNRPSAAELLRDPFFAGIDDDDS 322

[Close]

BLAST of Carg05144-RA vs. ExPASy Swiss-Prot
Match: Q9STK6 (Probable serine/threonine-protein kinase WNK3 OS=Arabidopsis thaliana OX=3702 GN=WNK3 PE=2 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 8.8e-98
Identity = 174/270 (64.44%), Postives = 212/270 (78.52%), Query Frame = 0


Query: 14  EPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLRKFSGDPVFINRLR 73
           E FVE+DPTGR+GRY ++LG GA K+VYRAFDQ EGIEVAWNQV+L         ++RL 
Sbjct: 10  EEFVEIDPTGRYGRYKEVLGKGAFKEVYRAFDQLEGIEVAWNQVKLDDKFCSSEDLDRLY 69

Query: 74  SEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKKHRHVSTKALKKWS 133
           SEV LL TL +  II  Y+ W D +H T+N ITEV TSGNLR YRKKH+ V  +ALKKWS
Sbjct: 70  SEVHLLKTLKHKSIIKFYTSWIDHQHMTINLITEVFTSGNLRQYRKKHKCVDLRALKKWS 129

Query: 134 KQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIVGKSHAAHSIIGTP 193
           +Q+LEGL YLH+H+P +IHRDL C NIF+NGN G+VKIGDLG AAI+ ++ +AHS+IGTP
Sbjct: 130 RQILEGLVYLHSHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAAILHRARSAHSVIGTP 189

Query: 194 EYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKKVTTGIKPQALNKV 253
           E+MAPELYEEDY  +VDIY+F MCLLE+VT E PYSEC + A+IY+KVT+GIKP AL  V
Sbjct: 190 EFMAPELYEEDYNVLVDIYAFGMCLLELVTFEYPYSECTNAAQIYRKVTSGIKPAALLNV 249

Query: 254 TNDEVRAFIEKCIAQPRARPTTSELLKDPF 284
           T+ +VRAFIEKCIA+   R +  ELL DPF
Sbjct: 250 TDPQVRAFIEKCIAKVSQRLSAKELLDDPF 279

[Close]

BLAST of Carg05144-RA vs. ExPASy Swiss-Prot
Match: Q65X23 (Probable serine/threonine-protein kinase WNK2 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK2 PE=2 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 1.9e-92
Identity = 168/286 (58.74%), Postives = 212/286 (74.13%), Query Frame = 0


Query: 12  DAEP-FVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLRKFSGDPVFIN 71
           D EP F EVDPT R+GRY ++LG GA K VY+AFDQ EG+EVAWNQ+++     +   + 
Sbjct: 13  DTEPEFAEVDPTARYGRYTEVLGKGAFKTVYKAFDQLEGLEVAWNQIKVGDILRNNDDLE 72

Query: 72  RLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKKHRHVSTKALK 131
           RLRSEV+LL TL +  II  Y+ W D ++N +NFITEV TSG LR YR KH+ V  +ALK
Sbjct: 73  RLRSEVRLLKTLKHKNIIKFYNSWLDKKNNNINFITEVFTSGTLRQYRIKHKKVDVRALK 132

Query: 132 KWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIVGKSHAAHSII 191
           KWS+Q+L GL YLH+H+P +IHRDL C NIFVNGN G+VKIGDLG A I+  + +AHSII
Sbjct: 133 KWSRQILSGLVYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLATILDNARSAHSII 192

Query: 192 GTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKKVTTGIKPQAL 251
           GTPE+MAPELY+E+Y E+VDIY+F MCLLE+VT E PY EC + A+IYKKV+ G KP +L
Sbjct: 193 GTPEFMAPELYDEEYNELVDIYAFGMCLLELVTFEYPYCECSNAAQIYKKVSDGEKPSSL 252

Query: 252 NKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY 297
            K+ + EVR FIEKCIA+   R +  ELL DPF   +RD+  +  Y
Sbjct: 253 AKIEDPEVRFFIEKCIAKASQRLSAQELLMDPF---LRDDGEKIFY 295

[Close]

BLAST of Carg05144-RA vs. ExPASy Swiss-Prot
Match: Q9CAV6 (Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=1 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 1.8e-90
Identity = 165/282 (58.51%), Postives = 206/282 (73.05%), Query Frame = 0


Query: 12  DAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLRKFSGDPVFINR 71
           D   FVEVDPTGR+GRY+++LG GA K VYRAFD+ EGIEVAWNQV+L  F   P  + R
Sbjct: 10  DYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDLER 69

Query: 72  LRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKKHRHVSTKALKK 131
           L  E+ LL TL +  I+  Y+ W D  +  +NF+TE+ TSG LR YR +H+ V+ +A+K 
Sbjct: 70  LYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAMKH 129

Query: 132 WSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIVGKSHAAHSIIG 191
           W +Q+L GL YLH+H+P +IHRDL C NIFVNGN G+VKIGDLG AAI+ KSHAAH  +G
Sbjct: 130 WCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAH-CVG 189

Query: 192 TPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKKVTTGIKPQALN 251
           TPE+MAPE+YEE Y E+VDIYSF MC+LEMVT + PYSEC   A+IYKKV +G KP AL 
Sbjct: 190 TPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDALY 249

Query: 252 KVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSE 294
           KV + EV+ FIEKC+A    R +  ELL DPF   +R +D E
Sbjct: 250 KVKDPEVKCFIEKCLATVSLRVSARELLDDPF---LRIDDGE 287

[Close]

BLAST of Carg05144-RA vs. ExPASy TrEMBL
Match: A0A6J1G507 (probable serine/threonine-protein kinase WNK11 OS=Cucurbita moschata OX=3662 GN=LOC111450971 PE=4 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 2.0e-169
Identity = 295/296 (99.66%), Postives = 295/296 (99.66%), Query Frame = 0


Query: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60
           MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR
Sbjct: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60

Query: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120
           KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK
Sbjct: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120

Query: 121 HRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180
           HRHVS KALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV
Sbjct: 121 HRHVSIKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180

Query: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240
           GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK
Sbjct: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240

Query: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY 297
           VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY
Sbjct: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY 296

[Close]

BLAST of Carg05144-RA vs. ExPASy TrEMBL
Match: A0A6J1I0Y1 (probable serine/threonine-protein kinase WNK11 OS=Cucurbita maxima OX=3661 GN=LOC111469844 PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.2e-168
Identity = 294/296 (99.32%), Postives = 294/296 (99.32%), Query Frame = 0


Query: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60
           MPADNSIPFDRDAEPFVEVDPTGRFGRYD LLGSGAVKKVYRAFDQEEGIEVAWNQVRLR
Sbjct: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDVLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60

Query: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120
           KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK
Sbjct: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120

Query: 121 HRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180
           HRHVS KALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV
Sbjct: 121 HRHVSIKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180

Query: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240
           GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK
Sbjct: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240

Query: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY 297
           VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY
Sbjct: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQSY 296

[Close]

BLAST of Carg05144-RA vs. ExPASy TrEMBL
Match: A0A1S3CKX0 (probable serine/threonine-protein kinase WNK11 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103502127 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 6.9e-162
Identity = 280/295 (94.92%), Postives = 289/295 (97.97%), Query Frame = 0


Query: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60
           MPA+NSIP+DRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR
Sbjct: 2   MPAENSIPYDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 61

Query: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120
            FSGDPVFINRLRSEVQLLSTL+N YIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK
Sbjct: 62  NFSGDPVFINRLRSEVQLLSTLNNKYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 121

Query: 121 HRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180
           HRHVS KALKKWSKQVLEGLDYLH HEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV
Sbjct: 122 HRHVSIKALKKWSKQVLEGLDYLHTHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 181

Query: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240
           G+SHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDS+AKIYKK
Sbjct: 182 GRSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSVAKIYKK 241

Query: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQS 296
           VTTGIKPQA+ KVT+DEVRAFIEKCIAQPRARP+ SELLKDPFFDEVRDEDSEQ+
Sbjct: 242 VTTGIKPQAITKVTDDEVRAFIEKCIAQPRARPSASELLKDPFFDEVRDEDSEQT 296

[Close]

BLAST of Carg05144-RA vs. ExPASy TrEMBL
Match: A0A5D3CKR4 (Putative serine/threonine-protein kinase WNK11 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold177G00650 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 6.9e-162
Identity = 280/295 (94.92%), Postives = 289/295 (97.97%), Query Frame = 0


Query: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60
           MPA+NSIP+DRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR
Sbjct: 1   MPAENSIPYDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60

Query: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120
            FSGDPVFINRLRSEVQLLSTL+N YIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK
Sbjct: 61  NFSGDPVFINRLRSEVQLLSTLNNKYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120

Query: 121 HRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180
           HRHVS KALKKWSKQVLEGLDYLH HEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV
Sbjct: 121 HRHVSIKALKKWSKQVLEGLDYLHTHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180

Query: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240
           G+SHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDS+AKIYKK
Sbjct: 181 GRSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSVAKIYKK 240

Query: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQS 296
           VTTGIKPQA+ KVT+DEVRAFIEKCIAQPRARP+ SELLKDPFFDEVRDEDSEQ+
Sbjct: 241 VTTGIKPQAITKVTDDEVRAFIEKCIAQPRARPSASELLKDPFFDEVRDEDSEQT 295

[Close]

BLAST of Carg05144-RA vs. ExPASy TrEMBL
Match: A0A1S3CMF1 (probable serine/threonine-protein kinase WNK11 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103502127 PE=4 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 6.9e-162
Identity = 280/295 (94.92%), Postives = 289/295 (97.97%), Query Frame = 0


Query: 1   MPADNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60
           MPA+NSIP+DRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR
Sbjct: 1   MPAENSIPYDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLR 60

Query: 61  KFSGDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120
            FSGDPVFINRLRSEVQLLSTL+N YIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK
Sbjct: 61  NFSGDPVFINRLRSEVQLLSTLNNKYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKK 120

Query: 121 HRHVSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180
           HRHVS KALKKWSKQVLEGLDYLH HEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV
Sbjct: 121 HRHVSIKALKKWSKQVLEGLDYLHTHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIV 180

Query: 181 GKSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKK 240
           G+SHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDS+AKIYKK
Sbjct: 181 GRSHAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSVAKIYKK 240

Query: 241 VTTGIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSEQS 296
           VTTGIKPQA+ KVT+DEVRAFIEKCIAQPRARP+ SELLKDPFFDEVRDEDSEQ+
Sbjct: 241 VTTGIKPQAITKVTDDEVRAFIEKCIAQPRARPSASELLKDPFFDEVRDEDSEQT 295

[Close]

BLAST of Carg05144-RA vs. TAIR 10
Match: AT5G55560.1 (Protein kinase superfamily protein )

HSP 1 Score: 467.2 bits (1201), Expect = 9.6e-132
Identity = 219/290 (75.52%), Postives = 253/290 (87.24%), Query Frame = 0


Query: 4   DNSIPFDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLRKFS 63
           DN    D+D+E FVEVDPTGR+GRY +LLGSGAVKKVYRAFDQEEGIEVAWNQV+LR FS
Sbjct: 9   DNESEKDKDSESFVEVDPTGRYGRYGELLGSGAVKKVYRAFDQEEGIEVAWNQVKLRCFS 68

Query: 64  GDPVFINRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKKHRH 123
            DP    RL SEV+LL  L N  II  Y VW D+ +NTLNFITE+CTSGNLR+YRKKHRH
Sbjct: 69  DDPAMTERLYSEVRLLKNLKNSNIITLYKVWRDERNNTLNFITEICTSGNLREYRKKHRH 128

Query: 124 VSTKALKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIVGKS 183
           VS +ALKKWSKQ+L+GLDYLH H+PCIIHRDLNCSNIFVNGNIGQVKIGDLG AAIVGK+
Sbjct: 129 VSMRALKKWSKQILKGLDYLHTHDPCIIHRDLNCSNIFVNGNIGQVKIGDLGLAAIVGKN 188

Query: 184 HAAHSIIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKKVTT 243
           H AHSI+GTPE+MAPELYEE+YTEMVDIYS+ MC+LE+V++EIPYSECDS+AKIYK+V+ 
Sbjct: 189 HLAHSILGTPEFMAPELYEENYTEMVDIYSYGMCVLELVSLEIPYSECDSVAKIYKRVSK 248

Query: 244 GIKPQALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSE 294
           G+KP+ALNKV + E +AFIEKCIAQPRARP+ +ELL DPFFD + D+D E
Sbjct: 249 GLKPEALNKVNDPEAKAFIEKCIAQPRARPSAAELLCDPFFDGILDDDDE 298

[Close]

BLAST of Carg05144-RA vs. TAIR 10
Match: AT3G48260.1 (with no lysine (K) kinase 3 )

HSP 1 Score: 358.2 bits (918), Expect = 6.3e-99
Identity = 174/270 (64.44%), Postives = 212/270 (78.52%), Query Frame = 0


Query: 14  EPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLRKFSGDPVFINRLR 73
           E FVE+DPTGR+GRY ++LG GA K+VYRAFDQ EGIEVAWNQV+L         ++RL 
Sbjct: 10  EEFVEIDPTGRYGRYKEVLGKGAFKEVYRAFDQLEGIEVAWNQVKLDDKFCSSEDLDRLY 69

Query: 74  SEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKKHRHVSTKALKKWS 133
           SEV LL TL +  II  Y+ W D +H T+N ITEV TSGNLR YRKKH+ V  +ALKKWS
Sbjct: 70  SEVHLLKTLKHKSIIKFYTSWIDHQHMTINLITEVFTSGNLRQYRKKHKCVDLRALKKWS 129

Query: 134 KQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIVGKSHAAHSIIGTP 193
           +Q+LEGL YLH+H+P +IHRDL C NIF+NGN G+VKIGDLG AAI+ ++ +AHS+IGTP
Sbjct: 130 RQILEGLVYLHSHDPPVIHRDLKCDNIFINGNQGEVKIGDLGLAAILHRARSAHSVIGTP 189

Query: 194 EYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKKVTTGIKPQALNKV 253
           E+MAPELYEEDY  +VDIY+F MCLLE+VT E PYSEC + A+IY+KVT+GIKP AL  V
Sbjct: 190 EFMAPELYEEDYNVLVDIYAFGMCLLELVTFEYPYSECTNAAQIYRKVTSGIKPAALLNV 249

Query: 254 TNDEVRAFIEKCIAQPRARPTTSELLKDPF 284
           T+ +VRAFIEKCIA+   R +  ELL DPF
Sbjct: 250 TDPQVRAFIEKCIAKVSQRLSAKELLDDPF 279

[Close]

BLAST of Carg05144-RA vs. TAIR 10
Match: AT3G04910.1 (with no lysine (K) kinase 1 )

HSP 1 Score: 334.0 bits (855), Expect = 1.3e-91
Identity = 165/282 (58.51%), Postives = 206/282 (73.05%), Query Frame = 0


Query: 12  DAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLRKFSGDPVFINR 71
           D   FVEVDPTGR+GRY+++LG GA K VYRAFD+ EGIEVAWNQV+L  F   P  + R
Sbjct: 10  DYSEFVEVDPTGRYGRYNEVLGKGASKTVYRAFDEYEGIEVAWNQVKLYDFLQSPEDLER 69

Query: 72  LRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKKHRHVSTKALKK 131
           L  E+ LL TL +  I+  Y+ W D  +  +NF+TE+ TSG LR YR +H+ V+ +A+K 
Sbjct: 70  LYCEIHLLKTLKHKNIMKFYTSWVDTANRNINFVTELFTSGTLRQYRLRHKRVNIRAMKH 129

Query: 132 WSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIVGKSHAAHSIIG 191
           W +Q+L GL YLH+H+P +IHRDL C NIFVNGN G+VKIGDLG AAI+ KSHAAH  +G
Sbjct: 130 WCRQILRGLHYLHSHDPPVIHRDLKCDNIFVNGNQGEVKIGDLGLAAILRKSHAAH-CVG 189

Query: 192 TPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKKVTTGIKPQALN 251
           TPE+MAPE+YEE Y E+VDIYSF MC+LEMVT + PYSEC   A+IYKKV +G KP AL 
Sbjct: 190 TPEFMAPEVYEEAYNELVDIYSFGMCILEMVTFDYPYSECTHPAQIYKKVMSGKKPDALY 249

Query: 252 KVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDSE 294
           KV + EV+ FIEKC+A    R +  ELL DPF   +R +D E
Sbjct: 250 KVKDPEVKCFIEKCLATVSLRVSARELLDDPF---LRIDDGE 287

[Close]

BLAST of Carg05144-RA vs. TAIR 10
Match: AT3G22420.1 (with no lysine (K) kinase 2 )

HSP 1 Score: 332.0 bits (850), Expect = 4.8e-91
Identity = 157/278 (56.47%), Postives = 207/278 (74.46%), Query Frame = 0


Query: 9   FDRDAEPFVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLRKFSGDPVF 68
           F  D   FVE+DP+GR+GRYD++LG GA K VYRAFD+ EGIEVAWNQV+LR F+ +P  
Sbjct: 7   FVEDCSVFVEIDPSGRYGRYDEILGKGASKTVYRAFDEYEGIEVAWNQVKLRNFTRNPEE 66

Query: 69  INRLRSEVQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKKHRHVSTKA 128
           + +   E+ LL TL++  I+  Y+ W D  + ++NF+TE+ TSG LR YR +HR V+ +A
Sbjct: 67  LEKFFREIHLLKTLNHQNIMKFYTSWVDTNNLSINFVTELFTSGTLRQYRLRHRRVNIRA 126

Query: 129 LKKWSKQVLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIVGKSHAAHS 188
           +K+W KQ+L+GL YLH+  P IIHRDL C NIF+NGN G+VKIGDLG AAI+ KSHA   
Sbjct: 127 VKQWCKQILKGLLYLHSRSPPIIHRDLKCDNIFINGNQGEVKIGDLGLAAILRKSHAVR- 186

Query: 189 IIGTPEYMAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKKVTTGIKPQ 248
            +GTPE+MAPE+Y+E+Y E+VD+Y+F MC+LEMVT + PYSEC   A+IYKKVT+G KP+
Sbjct: 187 CVGTPEFMAPEVYDEEYNELVDVYAFGMCVLEMVTFDYPYSECTHPAQIYKKVTSGKKPE 246

Query: 249 ALNKVTNDEVRAFIEKCIAQPRARPTTSELLKDPFFDE 287
           A   V + EVR F+EKC+A    R T  ELL+DPF  +
Sbjct: 247 AFYLVKDPEVREFVEKCLANVTCRLTALELLQDPFLQD 283

[Close]

BLAST of Carg05144-RA vs. TAIR 10
Match: AT5G41990.1 (with no lysine (K) kinase 8 )

HSP 1 Score: 325.5 bits (833), Expect = 4.5e-89
Identity = 159/277 (57.40%), Postives = 203/277 (73.29%), Query Frame = 0


Query: 16  FVEVDPTGRFGRYDDLLGSGAVKKVYRAFDQEEGIEVAWNQVRLRKFSGDPVFINRLRSE 75
           F E DP+GR+ RYDD+LG GA K VY+AFD+ +GIEVAWN V +      P  + RL SE
Sbjct: 19  FAEKDPSGRYIRYDDVLGRGAFKTVYKAFDEVDGIEVAWNLVSIEDVMQMPGQLERLYSE 78

Query: 76  VQLLSTLSNDYIIVCYSVWNDDEHNTLNFITEVCTSGNLRDYRKKHRHVSTKALKKWSKQ 135
           V LL  L ++ II  +  W D+++ T+N ITE+ TSG+LR YRKKHR V  KA+K W++Q
Sbjct: 79  VHLLKALKHENIIKLFYSWVDEKNKTINMITELFTSGSLRVYRKKHRKVDPKAIKNWARQ 138

Query: 136 VLEGLDYLHNHEPCIIHRDLNCSNIFVNGNIGQVKIGDLGFAAIVGKSHAAHSIIGTPEY 195
           +L+GL+YLH+  P +IHRDL C NIFVNGN G+VKIGDLG A ++ +   A S+IGTPE+
Sbjct: 139 ILKGLNYLHSQNPPVIHRDLKCDNIFVNGNTGEVKIGDLGLATVL-QQPTARSVIGTPEF 198

Query: 196 MAPELYEEDYTEMVDIYSFAMCLLEMVTMEIPYSECDSIAKIYKKVTTGIKPQALNKVTN 255
           MAPELYEE+Y E+VDIYSF MC+LEMVT E PY+EC + A+IYKKVT+ IKPQ+L KV +
Sbjct: 199 MAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYNECRNQAQIYKKVTSNIKPQSLGKVDD 258

Query: 256 DEVRAFIEKCIAQPRARPTTSELLKDPFFDEVRDEDS 293
            +VR FIEKC+    +RPT  EL KDPF      +DS
Sbjct: 259 PQVRQFIEKCLLPASSRPTALELSKDPFLARDGGKDS 294

The following BLAST results are available for this feature:

BLAST of Carg05144-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7026985.1	8.3e-170	100.00	putative serine/threonine-protein kinase WNK11, partial [Cucurbita argyrosperma ...	[more]
XP_022946942.1	4.1e-169	99.66	probable serine/threonine-protein kinase WNK11 [Cucurbita moschata] >XP_02294694...	[more]
XP_022971060.1	4.6e-168	99.32	probable serine/threonine-protein kinase WNK11 [Cucurbita maxima] >XP_022971061....	[more]
KAG6604902.1	1.2e-163	99.65	putative serine/threonine-protein kinase WNK11, partial [Cucurbita argyrosperma ...	[more]
XP_008464187.1	1.4e-161	94.92	PREDICTED: probable serine/threonine-protein kinase WNK11 isoform X1 [Cucumis me...	[more]

BLAST of Carg05144-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6ICW6	1.3e-130	75.52	Probable serine/threonine-protein kinase WNK11 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q0D541	1.0e-109	65.60	Probable serine/threonine-protein kinase WNK5 OS=Oryza sativa subsp. japonica OX...	[more]
Q9STK6	8.8e-98	64.44	Probable serine/threonine-protein kinase WNK3 OS=Arabidopsis thaliana OX=3702 GN...	[more]
Q65X23	1.9e-92	58.74	Probable serine/threonine-protein kinase WNK2 OS=Oryza sativa subsp. japonica OX...	[more]
Q9CAV6	1.8e-90	58.51	Serine/threonine-protein kinase WNK1 OS=Arabidopsis thaliana OX=3702 GN=WNK1 PE=...	[more]

BLAST of Carg05144-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G507	2.0e-169	99.66	probable serine/threonine-protein kinase WNK11 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1I0Y1	2.2e-168	99.32	probable serine/threonine-protein kinase WNK11 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A1S3CKX0	6.9e-162	94.92	probable serine/threonine-protein kinase WNK11 isoform X1 OS=Cucumis melo OX=365...	[more]
A0A5D3CKR4	6.9e-162	94.92	Putative serine/threonine-protein kinase WNK11 isoform X2 OS=Cucumis melo var. m...	[more]
A0A1S3CMF1	6.9e-162	94.92	probable serine/threonine-protein kinase WNK11 isoform X2 OS=Cucumis melo OX=365...	[more]

BLAST of Carg05144-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55560.1	9.6e-132	75.52	Protein kinase superfamily protein	[more]
AT3G48260.1	6.3e-99	64.44	with no lysine (K) kinase 3	[more]
AT3G04910.1	1.3e-91	58.51	with no lysine (K) kinase 1	[more]
AT3G22420.1	4.8e-91	56.47	with no lysine (K) kinase 2	[more]
AT5G41990.1	4.5e-89	57.40	with no lysine (K) kinase 8	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 109..290 e-value: 1.0E-50 score: 173.8
None	No IPR available	GENE3D	3.30.200.20	Phosphorylase Kinase; domain 1	coord: 11..107 e-value: 3.1E-27 score: 96.4
None	No IPR available	PIRSR	PIRSR000620-2	PIRSR000620-2	coord: 107..288 e-value: 1.7E-10 score: 38.2
None	No IPR available	PANTHER	PTHR13902:SF137	SERINE/THREONINE-PROTEIN KINASE WNK11-RELATED	coord: 1..288
None	No IPR available	PANTHER	PTHR13902	SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATED	coord: 1..288
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 30..284 e-value: 4.6E-51 score: 173.7
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 26..284 score: 39.240944
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 19..285

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg05144	Carg05144	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg05144-RA:1	Carg05144-RA:1	exon
Carg05144-RA:2	Carg05144-RA:2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg05144-RA:cds	Carg05144-RA:cds_2	CDS
Carg05144-RA:cds	Carg05144-RA:cds	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg05144-RA	Carg05144-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0035556	intracellular signal transduction
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity
molecular_function	GO:0004674	protein serine/threonine kinase activity