Carg03912-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg03912-RA
TypemRNA
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptiontRNA (Cytosine(38)-C(5))-methyltransferase
LocationCarg_Chr12: 1991424 .. 1992677 (-)
Sequence length306
RNA-Seq ExpressionCarg03912-RA
SyntenyCarg03912-RA
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGGCATTTCCAAAGCAGCAAGACGAACATTGGAGAGTCCTTGAATTCTTCAGCGGCATTGGTGGCATAGTACTCTTTTCGCCTATGCTTCTCTCTGTATCCTCCTCGATCTCAGGCGGATATCGTACAGAGGCTTTGTTTGCGATTTCAATTATAACAGAGATACTCTCTACTGAGTGCTGGCGTCGATGCGAAAGTAGTACGAGCTTTTGATATCAACGATAAAGCAAACGACGTTTACGAGCATAATTTCGGCGATCGTCCGCATCAGGTATGATAGAATCATTTTCTTCTTATATCGGTTTGAGAACGCCTTGCTTTCTGCCGTATGAATAAGTATTCGGTGCTTCTCAGTGAAAACATTGAAACTAGTTTTCCTTCAAAATCGAATGCAAATTCTCTTCAGTTGTGGTTTAAGTAGATTGAATTTGAGGCTGTTGAATGTTGTAGCCAAGCTTATTTAATTAGTGAGGAACTACCTCCTGGATACTGATGCACGTATGGTATGATAAGCTTTCAATTTACAGCTAGTATGAATTCACAATGTGAGCGGACAATAGATAGGTACATGAAGTGTTTTTGTTGGAGTATCTGATTCAAATGACTGCCGTTGTGGACTTTCTTTTGAACTGCCAAAGTAAAAGCGTTCTACAATCTCTCAACATCGTCTTCTTCTTTTATTCCTTGTTTCGAGAACGATTATGTTGCTTGTCCATCGCAGGAGTAAAGTTTTGCTGCTTCTCAATACAGATAACGGAAGTATTTTGTTCATTCAGAATTAAGATGCCAATTCTTTTCTATCGTGGATTAAGTAGATTGAATTTGAAGTTATTGAATGTTGAAACATAGCTTATCTATTTCGTTGGAAATTACCTACCTCTGACCGATGGACGTATTTGTTAAGTGATTAGATTACAATTTGAAGCTCGTATGAACTCTCAACGTAGTGGAGAACAAGGTAGCTATATGACGTATTTTTGTTGGAATCTCTTATTCAAATGATTAGCGTAGTGAATTTTTTCTTTCATCGATTCGTCAAACCAAAAGCGTCATGCTATTTAGGAACATGTAACAGGTCATTCATAGCTTCCTTTGCATAGGAGTGTCGGTTTTTTCTTATGAGTTTAGTTATGGTCAAAGCAATCCTACGTGCTTGGTCAAGCTACTGTAAAGACTCAGAGAGCAACAGAGAGCTACAAGAAAGAGGCAGAACTCGAGTCTAAAAAACGAATCATGGAGAGACCTTGA

mRNA sequence

ATGGAGAAGGCATTTCCAAAGCAGCAAGACGAACATTGGAGAGTCCTTGAATTCTTCAGCGGCATTGGTGGCATAAGATACTCTCTACTGAGTGCTGGCGTCGATGCGAAAGTAGTACGAGCTTTTGATATCAACGATAAAGCAAACGACGTTTACGAGCATAATTTCGGCGATCGTCCGCATCAGTTATGGTCAAAGCAATCCTACGTGCTTGGTCAAGCTACTGTAAAGACTCAGAGAGCAACAGAGAGCTACAAGAAAGAGGCAGAACTCGAGTCTAAAAAACGAATCATGGAGAGACCTTGA

Coding sequence (CDS)

ATGGAGAAGGCATTTCCAAAGCAGCAAGACGAACATTGGAGAGTCCTTGAATTCTTCAGCGGCATTGGTGGCATAAGATACTCTCTACTGAGTGCTGGCGTCGATGCGAAAGTAGTACGAGCTTTTGATATCAACGATAAAGCAAACGACGTTTACGAGCATAATTTCGGCGATCGTCCGCATCAGTTATGGTCAAAGCAATCCTACGTGCTTGGTCAAGCTACTGTAAAGACTCAGAGAGCAACAGAGAGCTACAAGAAAGAGGCAGAACTCGAGTCTAAAAAACGAATCATGGAGAGACCTTGA

Protein sequence

MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRPHQLWSKQSYVLGQATVKTQRATESYKKEAELESKKRIMERP
Homology
[Close]
BLAST of Carg03912-RA vs. NCBI nr
Match: KAG7020373.1 (DNA (cytosine-5)-methyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 206.1 bits (523), Expect = 1.5e-49
Identity = 101/101 (100.00%), Postives = 101/101 (100.00%), Query Frame = 0 

Query: 1   MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
           MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP
Sbjct: 1   MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60

Query: 61  HQLWSKQSYVLGQATVKTQRATESYKKEAELESKKRIMERP 102
           HQLWSKQSYVLGQATVKTQRATESYKKEAELESKKRIMERP
Sbjct: 61  HQLWSKQSYVLGQATVKTQRATESYKKEAELESKKRIMERP 101

[Close]
BLAST of Carg03912-RA vs. NCBI nr
Match: KAG6585458.1 (tRNA (cytosine(38)-C(5))-methyltransferase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 137.9 bits (346), Expect = 4.9e-29
Identity = 64/72 (88.89%), Postives = 67/72 (93.06%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60

Query: 61 HQLWSKQSYVLG 73
          HQ++     +LG
Sbjct: 61 HQVYKSIPAMLG 72

[Close]
BLAST of Carg03912-RA vs. NCBI nr
Match: KAA0064954.1 (tRNA (cytosine(38)-C(5))-methyltransferase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 117.1 bits (292), Expect = 8.9e-23
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK FPK QDE WRVLEF+SGIGG+RYSLLSAG+ AKVV+AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 63
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Carg03912-RA vs. NCBI nr
Match: XP_008445162.1 (PREDICTED: tRNA (cytosine(38)-C(5))-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 117.1 bits (292), Expect = 8.9e-23
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK FPK QDE WRVLEF+SGIGG+RYSLLSAG+ AKVV+AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 63
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Carg03912-RA vs. NCBI nr
Match: XP_004138735.1 (tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 117.1 bits (292), Expect = 8.9e-23
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK FPK QDE WRVLEF+SGIGG+RYSLLSAG+ AKVV+AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 63
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Carg03912-RA vs. ExPASy Swiss-Prot
Match: F4JWT7 (tRNA (cytosine(38)-C(5))-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=DNMT2 PE=1 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 1.2e-14
Identity = 33/50 (66.00%), Postives = 45/50 (90.00%), Query Frame = 0 

Query: 13 WRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRPHQ 63
          W+VLEF+SGIGG+RYSL+++G+ ++VV AF+IND ANDVY+HNF  RP+Q
Sbjct: 15 WQVLEFYSGIGGMRYSLMASGIVSEVVEAFEINDSANDVYQHNFKHRPYQ 64

[Close]
BLAST of Carg03912-RA vs. ExPASy Swiss-Prot
Match: Q54JH6 (DNA (cytosine-5)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=dnmA PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.1e-07
Identity = 26/52 (50.00%), Postives = 36/52 (69.23%), Query Frame = 0 

Query: 11 EHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRPHQ 63
          E  RVLEF+SGIGG+ Y L  +GVD +V+++FDIN  AN  Y++ F +   Q
Sbjct: 2  EQLRVLEFYSGIGGMHYGLQESGVDFQVIQSFDINTNANLNYKYTFNEDSSQ 53

[Close]
BLAST of Carg03912-RA vs. ExPASy Swiss-Prot
Match: O55055 (tRNA (cytosine(38)-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Trdmt1 PE=1 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 9.3e-07
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 0 

Query: 11 EHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRPH 62
          E  RVLE +SGIGG+ ++L  + + A VV A D+N  AN+VY+HNF   PH
Sbjct: 2  EPLRVLELYSGIGGMHHALRESHIPAHVVAAIDVNTVANEVYKHNF---PH 49

[Close]
BLAST of Carg03912-RA vs. ExPASy Swiss-Prot
Match: Q4G073 (tRNA (cytosine(38)-C(5))-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trdmt1 PE=2 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.7e-06
Identity = 26/51 (50.98%), Postives = 35/51 (68.63%), Query Frame = 0 

Query: 11 EHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRPH 62
          E  RVLE +SGIGG+ ++L  + V A VV A D++  AN+VY+HNF   PH
Sbjct: 2  EPLRVLELYSGIGGMHHALRESRVPAHVVAAIDVSTVANEVYKHNF---PH 49

[Close]
BLAST of Carg03912-RA vs. ExPASy Swiss-Prot
Match: O14717 (tRNA (cytosine(38)-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=TRDMT1 PE=1 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 3.5e-06
Identity = 27/57 (47.37%), Postives = 40/57 (70.18%), Query Frame = 0 

Query: 11 EHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRPH-QLWSK 67
          E  RVLE +SG+GG+ ++L  + + A+VV A D+N  AN+VY++NF   PH QL +K
Sbjct: 2  EPLRVLELYSGVGGMHHALRESCIPAQVVAAIDVNTVANEVYKYNF---PHTQLLAK 55

[Close]
BLAST of Carg03912-RA vs. ExPASy TrEMBL
Match: A0A5A7VCE7 (tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002740 PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.3e-23
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK FPK QDE WRVLEF+SGIGG+RYSLLSAG+ AKVV+AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 63
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Carg03912-RA vs. ExPASy TrEMBL
Match: A0A0A0LSE8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G380010 PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.3e-23
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK FPK QDE WRVLEF+SGIGG+RYSLLSAG+ AKVV+AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 63
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Carg03912-RA vs. ExPASy TrEMBL
Match: A0A1S3BC07 (tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488282 PE=3 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 4.3e-23
Identity = 53/62 (85.48%), Postives = 58/62 (93.55%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK FPK QDE WRVLEF+SGIGG+RYSLLSAG+ AKVV+AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVFPKLQDEPWRVLEFYSGIGGMRYSLLSAGIHAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 63
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Carg03912-RA vs. ExPASy TrEMBL
Match: A0A6J1BVV7 (DNA (Cytosine-5)-methyltransferase isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005184 PE=3 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 8.1e-22
Identity = 52/62 (83.87%), Postives = 57/62 (91.94%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK  P+ Q E WRVLEF+SGIGG+RYSLLSAGVDAKVV+AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVSPQLQGEPWRVLEFYSGIGGMRYSLLSAGVDAKVVQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 63
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Carg03912-RA vs. ExPASy TrEMBL
Match: A0A6J1K9S0 (tRNA (Cytosine(38)-C(5))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111491952 PE=3 SV=1)

HSP 1 Score: 111.7 bits (278), Expect = 1.8e-21
Identity = 50/62 (80.65%), Postives = 56/62 (90.32%), Query Frame = 0 

Query: 1  MEKAFPKQQDEHWRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRP 60
          MEK  PK Q E WRVLEF+SGIGG+RYSLL AG+DAKV++AFDINDKANDVYEHNFGDRP
Sbjct: 1  MEKVSPKLQGEPWRVLEFYSGIGGMRYSLLRAGIDAKVIQAFDINDKANDVYEHNFGDRP 60

Query: 61 HQ 63
          +Q
Sbjct: 61 YQ 62

[Close]
BLAST of Carg03912-RA vs. TAIR 10
Match: AT5G25480.1 (DNA methyltransferase-2 )

HSP 1 Score: 80.5 bits (197), Expect = 8.6e-16
Identity = 33/50 (66.00%), Postives = 45/50 (90.00%), Query Frame = 0 

Query: 13 WRVLEFFSGIGGIRYSLLSAGVDAKVVRAFDINDKANDVYEHNFGDRPHQ 63
          W+VLEF+SGIGG+RYSL+++G+ ++VV AF+IND ANDVY+HNF  RP+Q
Sbjct: 15 WQVLEFYSGIGGMRYSLMASGIVSEVVEAFEINDSANDVYQHNFKHRPYQ 64

The following BLAST results are available for this feature:
BLAST of Carg03912-RA vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7020373.11.5e-49100.00DNA (cytosine-5)-methyltransferase, partial [Cucurbita argyrosperma subsp. argyr... [more]
KAG6585458.14.9e-2988.89tRNA (cytosine(38)-C(5))-methyltransferase 2, partial [Cucurbita argyrosperma su... [more]
KAA0064954.18.9e-2385.48tRNA (cytosine(38)-C(5))-methyltransferase isoform X1 [Cucumis melo var. makuwa][more]
XP_008445162.18.9e-2385.48PREDICTED: tRNA (cytosine(38)-C(5))-methyltransferase isoform X1 [Cucumis melo][more]
XP_004138735.18.9e-2385.48tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X2 [Cucumis sativus][more]
BLAST of Carg03912-RA vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4JWT71.2e-1466.00tRNA (cytosine(38)-C(5))-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q54JH61.1e-0750.00DNA (cytosine-5)-methyltransferase OS=Dictyostelium discoideum OX=44689 GN=dnmA ... [more]
O550559.3e-0750.98tRNA (cytosine(38)-C(5))-methyltransferase OS=Mus musculus OX=10090 GN=Trdmt1 PE... [more]
Q4G0732.7e-0650.98tRNA (cytosine(38)-C(5))-methyltransferase OS=Rattus norvegicus OX=10116 GN=Trdm... [more]
O147173.5e-0647.37tRNA (cytosine(38)-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=TRDMT1 PE=... [more]
BLAST of Carg03912-RA vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7VCE74.3e-2385.48tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo var. makuw... [more]
A0A0A0LSE84.3e-2385.48Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G380010 PE=3 SV=1[more]
A0A1S3BC074.3e-2385.48tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN... [more]
A0A6J1BVV78.1e-2283.87DNA (Cytosine-5)-methyltransferase isoform X1 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1K9S01.8e-2180.65tRNA (Cytosine(38)-C(5))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
BLAST of Carg03912-RA vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G25480.18.6e-1666.00DNA methyltransferase-2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001525C-5 cytosine methyltransferasePFAMPF00145DNA_methylasecoord: 14..60
e-value: 1.2E-6
score: 28.2
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 4..92
e-value: 6.1E-13
score: 50.3
NoneNo IPR availablePANTHERPTHR46098TRNA (CYTOSINE(38)-C(5))-METHYLTRANSFERASEcoord: 10..62
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 13..65

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Carg03912Carg03912gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg03912-RA:1Carg03912-RA:1exon
Carg03912-RA:2Carg03912-RA:2exon
Carg03912-RA:3Carg03912-RA:3exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Carg03912-RA:cdsCarg03912-RA:cds_3CDS
Carg03912-RA:cdsCarg03912-RA:cds_2CDS
Carg03912-RA:cdsCarg03912-RA:cdsCDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Carg03912-RACarg03912-RA-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
cellular_component GO:0005634 nucleus
molecular_function GO:0008168 methyltransferase activity