Carg01857-RA (mRNA) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01857-RA
Type	mRNA
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Auxin-responsive protein
Location	Carg_Chr04: 8975053 .. 8976027 (+)
Sequence length	704
RNA-Seq Expression	Carg01857-RA
Synteny	Carg01857-RA

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCATTTGAGATAATGGAGCCTAATCAGTTCTCTGTTTACTGTGAAGGGAAAGGGGAGAATGGGAGCATTGATTTGGGGCTGAGCCTGAGAACGCTGCAGCCAGAGGTGGTGGGATCAATAGAAGGGTATGGGGAGGAGGATGAGCATGGGATCAACTGGGGGGCGGCGGATCACACGGCGGCGGATCTGTACATGAACATCAACATCAACATGAAACATAGTAATAATAATAATGGAGTTGTAGTAGTACAACAAGATTACTACTGTTGTGCGGAGGAGGAGGAGGAGGAGGCAGAGGAGGTGGTGCAGAGCAAACAGCAGCGCTGGGCTTATGTCAAAGTCAATATGGATGGTGTCATTGTTGGTAGAAAAGTTTGCATCTTTCAACATGGCTCCTATTCCAGTCTCGCGCTTCAGCTCGAAGACATGTTTGGTATGGTTCATTTCTTTTTGTCGATCATAATTTGACGTGTTAAGGAGTGGATAAAGCTTAATTTTGATATGGGTCTCTCTTTTTTGTTGGTTAGGTTAGTTTGGTTTGTTCGAGTTATTTATTTAAATACTTATACTGTCTATGTTTATTGGTGGGATCAGGTCGACAATGTGAATCTGGGTTGAAATTGTTTGAGAATGAATCTGAATTCTCACTCTTTTACAAAGATGGAGACGAAAATTGGAGGAGTGTCGGTGACGTCCCGTGGAAGTAAGTTTATAACAATCGTTCGATTTACTTAAATGAATCAAATTAATTTGAGTGTTAGTTTTAAATTTTTGATCTAATTTGGTGACTTGGTTTTGTTGGTAAACGGACAGGCAATTCGTGGAGGATGTAAAGAGGCTGAGAATTGCTCGGAAAGATGAGGGTTTTGTTGCCATTCATCAAAATTAACTTAAAAACTTTCTTAATGGAATTATATTTTGTGTTACGTTGGAAGCTTTGGATAAATTATCTAATTTTAAGGAACCTTTTATAAC

mRNA sequence

CCATTTGAGATAATGGAGCCTAATCAGTTCTCTGTTTACTGTGAAGGGAAAGGGGAGAATGGGAGCATTGATTTGGGGCTGAGCCTGAGAACGCTGCAGCCAGAGGTGGTGGGATCAATAGAAGGGTATGGGGAGGAGGATGAGCATGGGATCAACTGGGGGGCGGCGGATCACACGGCGGCGGATCTGTACATGAACATCAACATCAACATGAAACATAGTAATAATAATAATGGAGTTGTAGTAGTACAACAAGATTACTACTGTTGTGCGGAGGAGGAGGAGGAGGAGGCAGAGGAGGTGGTGCAGAGCAAACAGCAGCGCTGGGCTTATGTCAAAGTCAATATGGATGGTGTCATTGTTGGTAGAAAAGTTTGCATCTTTCAACATGGCTCCTATTCCAGTCTCGCGCTTCAGCTCGAAGACATGTTTGGTCGACAATGTGAATCTGGGTTGAAATTGTTTGAGAATGAATCTGAATTCTCACTCTTTTACAAAGATGGAGACGAAAATTGGAGGAGTGTCGGTGACGTCCCGTGGAAGCAATTCGTGGAGGATGTAAAGAGGCTGAGAATTGCTCGGAAAGATGAGGGTTTTGTTGCCATTCATCAAAATTAACTTAAAAACTTTCTTAATGGAATTATATTTTGTGTTACGTTGGAAGCTTTGGATAAATTATCTAATTTTAAGGAACCTTTTATAAC

Coding sequence (CDS)

CCATTTGAGATAATGGAGCCTAATCAGTTCTCTGTTTACTGTGAAGGGAAAGGGGAGAATGGGAGCATTGATTTGGGGCTGAGCCTGAGAACGCTGCAGCCAGAGGTGGTGGGATCAATAGAAGGGTATGGGGAGGAGGATGAGCATGGGATCAACTGGGGGGCGGCGGATCACACGGCGGCGGATCTGTACATGAACATCAACATCAACATGAAACATAGTAATAATAATAATGGAGTTGTAGTAGTACAACAAGATTACTACTGTTGTGCGGAGGAGGAGGAGGAGGAGGCAGAGGAGGTGGTGCAGAGCAAACAGCAGCGCTGGGCTTATGTCAAAGTCAATATGGATGGTGTCATTGTTGGTAGAAAAGTTTGCATCTTTCAACATGGCTCCTATTCCAGTCTCGCGCTTCAGCTCGAAGACATGTTTGGTCGACAATGTGAATCTGGGTTGAAATTGTTTGAGAATGAATCTGAATTCTCACTCTTTTACAAAGATGGAGACGAAAATTGGAGGAGTGTCGGTGACGTCCCGTGGAAGCAATTCGTGGAGGATGTAAAGAGGCTGAGAATTGCTCGGAAAGATGAGGGTTTTGTTGCCATTCATCAAAATTAA

Protein sequence

PFEIMEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLYMNININMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRKVCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFVEDVKRLRIARKDEGFVAIHQN

Homology

[Close]

BLAST of Carg01857-RA vs. NCBI nr
Match: KAG7032061.1 (Auxin-responsive protein IAA32, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 426.4 bits (1095), Expect = 1.4e-115
Identity = 205/205 (100.00%), Postives = 205/205 (100.00%), Query Frame = 0


Query: 1   PFEIMEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTA 60
           PFEIMEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTA
Sbjct: 1   PFEIMEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTA 60

Query: 61  ADLYMNININMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQQRWAYVKVNMDGVI 120
           ADLYMNININMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQQRWAYVKVNMDGVI
Sbjct: 61  ADLYMNININMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQQRWAYVKVNMDGVI 120

Query: 121 VGRKVCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPW 180
           VGRKVCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPW
Sbjct: 121 VGRKVCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPW 180

Query: 181 KQFVEDVKRLRIARKDEGFVAIHQN 206
           KQFVEDVKRLRIARKDEGFVAIHQN
Sbjct: 181 KQFVEDVKRLRIARKDEGFVAIHQN 205

[Close]

BLAST of Carg01857-RA vs. NCBI nr
Match: KAG6601273.1 (Auxin-responsive protein IAA32, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 410.2 bits (1053), Expect = 1.0e-110
Identity = 199/201 (99.00%), Postives = 200/201 (99.50%), Query Frame = 0


Query: 5   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY 64
           MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY
Sbjct: 1   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY 60

Query: 65  MNININMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK 124
           MNIN+NMKHSNNNNGVVVVQQDYYCCA EEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK
Sbjct: 61  MNINMNMKHSNNNNGVVVVQQDYYCCA-EEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK 120

Query: 125 VCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFV 184
           VCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFV
Sbjct: 121 VCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFV 180

Query: 185 EDVKRLRIARKDEGFVAIHQN 206
           EDVKRLRIARKDEGFVAIHQN
Sbjct: 181 EDVKRLRIARKDEGFVAIHQN 200

[Close]

BLAST of Carg01857-RA vs. NCBI nr
Match: XP_022957191.1 (auxin-responsive protein IAA32-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 407.1 bits (1045), Expect = 8.7e-110
Identity = 197/201 (98.01%), Postives = 199/201 (99.00%), Query Frame = 0


Query: 5   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY 64
           MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY
Sbjct: 1   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY 60

Query: 65  MNININMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK 124
           MNININMKHSNNNNGVVVV+QDYYCC  EEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK
Sbjct: 61  MNININMKHSNNNNGVVVVEQDYYCC--EEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK 120

Query: 125 VCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFV 184
           VCIFQHGSYS+LALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFV
Sbjct: 121 VCIFQHGSYSNLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFV 180

Query: 185 EDVKRLRIARKDEGFVAIHQN 206
           EDVKRLRIARKDEGFVAIHQN
Sbjct: 181 EDVKRLRIARKDEGFVAIHQN 199

[Close]

BLAST of Carg01857-RA vs. NCBI nr
Match: XP_022957190.1 (auxin-responsive protein IAA32-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 402.1 bits (1032), Expect = 2.8e-108
Identity = 197/203 (97.04%), Postives = 199/203 (98.03%), Query Frame = 0


Query: 5   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY 64
           MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY
Sbjct: 1   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY 60

Query: 65  MNININMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK 124
           MNININMKHSNNNNGVVVV+QDYYCC  EEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK
Sbjct: 61  MNININMKHSNNNNGVVVVEQDYYCC--EEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK 120

Query: 125 VCIFQHGSYSSLALQLEDMF--GRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQ 184
           VCIFQHGSYS+LALQLEDMF  GRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQ
Sbjct: 121 VCIFQHGSYSNLALQLEDMFGSGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQ 180

Query: 185 FVEDVKRLRIARKDEGFVAIHQN 206
           FVEDVKRLRIARKDEGFVAIHQN
Sbjct: 181 FVEDVKRLRIARKDEGFVAIHQN 201

[Close]

BLAST of Carg01857-RA vs. NCBI nr
Match: XP_022986212.1 (auxin-responsive protein IAA32-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 402.1 bits (1032), Expect = 2.8e-108
Identity = 197/203 (97.04%), Postives = 199/203 (98.03%), Query Frame = 0


Query: 5   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY 64
           MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHG+NWGAADHTAADLY
Sbjct: 1   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGMNWGAADHTAADLY 60

Query: 65  --MNININMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQQRWAYVKVNMDGVIVG 124
             MNININMKHSNNNNGVVVV+QDYYCC  EEEEEAEEVVQSKQQRWAYVKVNMDGVIVG
Sbjct: 61  MNMNININMKHSNNNNGVVVVEQDYYCC--EEEEEAEEVVQSKQQRWAYVKVNMDGVIVG 120

Query: 125 RKVCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQ 184
           RKVCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQ
Sbjct: 121 RKVCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQ 180

Query: 185 FVEDVKRLRIARKDEGFVAIHQN 206
           FVEDVKRLRIARKDEGFVAIHQN
Sbjct: 181 FVEDVKRLRIARKDEGFVAIHQN 201

[Close]

BLAST of Carg01857-RA vs. ExPASy Swiss-Prot
Match: Q8RYC6 (Auxin-responsive protein IAA32 OS=Arabidopsis thaliana OX=3702 GN=IAA32 PE=1 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 1.8e-33
Identity = 84/194 (43.30%), Postives = 116/194 (59.79%), Query Frame = 0


Query: 13  YCEGKGENGSIDLGLSLRTLQ------PEVVGSIEGYGEEDEHGINWGAADHTAADLYMN 72
           Y + K   G IDLGLSLRT+Q      P  +  ++GYGE     I+W    +        
Sbjct: 20  YSQTKKGGGVIDLGLSLRTIQHETYLPPARMIGLDGYGEL----IDWSQPSY-------- 79

Query: 73  ININMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRKVC 132
            N   +  + + G   + Q YY        E E      + ++AYVKVN+DG++VGRKVC
Sbjct: 80  -NSITQLKSEDTGHQRLAQGYY------NNEGE-----SRGKYAYVKVNLDGLVVGRKVC 139

Query: 133 IFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFVED 192
           +   G+Y++LALQL DMFG Q  SGL+LF+ ESEFSL Y+D +  WR+VGDVPWK+FVE 
Sbjct: 140 LVDQGAYATLALQLNDMFGMQTVSGLRLFQTESEFSLVYRDREGIWRNVGDVPWKEFVES 189

Query: 193 VKRLRIARKDEGFV 201
           V R+RIAR+++  +
Sbjct: 200 VDRMRIARRNDALL 189

[Close]

BLAST of Carg01857-RA vs. ExPASy Swiss-Prot
Match: Q9C5X0 (Auxin-responsive protein IAA34 OS=Arabidopsis thaliana OX=3702 GN=IAA34 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 4.6e-29
Identity = 56/92 (60.87%), Postives = 74/92 (80.43%), Query Frame = 0


Query: 106 QQRWAYVKVNMDGVIVGRKVCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFY 165
           +++W YVKV MDG++VGRKVC+  HGSYS+LA QLEDMFG Q  SGL+LF+ ESEF L Y
Sbjct: 89  RRKWGYVKVTMDGLVVGRKVCVLDHGSYSTLAHQLEDMFGMQSVSGLRLFQMESEFCLVY 148

Query: 166 KDGDENWRSVGDVPWKQFVEDVKRLRIARKDE 198
           +D +  WR+ GDVPW +F+E V+RLRI R+++
Sbjct: 149 RDEEGLWRNAGDVPWNEFIESVERLRITRRND 180

[Close]

BLAST of Carg01857-RA vs. ExPASy Swiss-Prot
Match: A2XB18 (Auxin-responsive protein IAA10 OS=Oryza sativa subsp. indica OX=39946 GN=IAA10 PE=2 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 3.1e-17
Identity = 51/114 (44.74%), Postives = 70/114 (61.40%), Query Frame = 0


Query: 92  EEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRKVCIFQHGSYSSLALQLEDMFGRQ---- 151
           E + ++ +E  + K +   +VKVNMDG ++GRKV +  H SY +LAL LE MF +     
Sbjct: 146 ESDVQKDKEEGEKKGRVAGWVKVNMDGEVIGRKVDLNAHRSYKTLALALELMFTKPSIGL 205

Query: 152 CES----GLKLFENESEFSLFYKDGDENWRSVGDVPWKQFVEDVKRLRIARKDE 198
           C S     LKL +N +E+ L Y+D D +W  VGDVPW+ FV  VKRLRI R  +
Sbjct: 206 CASHNTNSLKLLDNSAEYQLTYEDRDGDWMLVGDVPWEMFVSSVKRLRIMRTSD 259

[Close]

BLAST of Carg01857-RA vs. ExPASy Swiss-Prot
Match: Q0DWF2 (Auxin-responsive protein IAA10 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA10 PE=2 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 3.1e-17
Identity = 51/114 (44.74%), Postives = 70/114 (61.40%), Query Frame = 0


Query: 92  EEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRKVCIFQHGSYSSLALQLEDMFGRQ---- 151
           E + ++ +E  + K +   +VKVNMDG ++GRKV +  H SY +LAL LE MF +     
Sbjct: 146 ESDVQKDKEEGEKKGRVAGWVKVNMDGEVIGRKVDLNAHRSYKTLALALELMFTKPSIGL 205

Query: 152 CES----GLKLFENESEFSLFYKDGDENWRSVGDVPWKQFVEDVKRLRIARKDE 198
           C S     LKL +N +E+ L Y+D D +W  VGDVPW+ FV  VKRLRI R  +
Sbjct: 206 CASHNTNSLKLLDNSAEYQLTYEDRDGDWMLVGDVPWEMFVSSVKRLRIMRTSD 259

[Close]

BLAST of Carg01857-RA vs. ExPASy Swiss-Prot
Match: Q38831 (Auxin-responsive protein IAA13 OS=Arabidopsis thaliana OX=3702 GN=IAA13 PE=1 SV=2)

HSP 1 Score: 89.0 bits (219), Expect = 6.9e-17
Identity = 49/116 (42.24%), Postives = 68/116 (58.62%), Query Frame = 0


Query: 92  EEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRKVCIFQHGSYSSLALQLEDMFGR----- 151
           ++E ++  + V  K Q   ++KVNMDGV +GRKV +  H SY +LA  LEDMF R     
Sbjct: 113 DDEPKDVTKKVNGKVQ-VGFIKVNMDGVAIGRKVDLNAHSSYENLAQTLEDMFFRTNPGT 172

Query: 152 -----QCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFVEDVKRLRIARKDE 198
                Q    L+L +  SEF L Y+D + +W  VGDVPW+ F+  VKRLR+ +  E
Sbjct: 173 VGLTSQFTKPLRLLDGSSEFVLTYEDKEGDWMLVGDVPWRMFINSVKRLRVMKTSE 227

[Close]

BLAST of Carg01857-RA vs. ExPASy TrEMBL
Match: A0A6J1GYJ4 (Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111458660 PE=3 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 4.2e-110
Identity = 197/201 (98.01%), Postives = 199/201 (99.00%), Query Frame = 0


Query: 5   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY 64
           MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY
Sbjct: 1   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY 60

Query: 65  MNININMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK 124
           MNININMKHSNNNNGVVVV+QDYYCC  EEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK
Sbjct: 61  MNININMKHSNNNNGVVVVEQDYYCC--EEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK 120

Query: 125 VCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFV 184
           VCIFQHGSYS+LALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFV
Sbjct: 121 VCIFQHGSYSNLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFV 180

Query: 185 EDVKRLRIARKDEGFVAIHQN 206
           EDVKRLRIARKDEGFVAIHQN
Sbjct: 181 EDVKRLRIARKDEGFVAIHQN 199

[Close]

BLAST of Carg01857-RA vs. ExPASy TrEMBL
Match: A0A6J1J6Y8 (Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111483950 PE=3 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.4e-108
Identity = 197/203 (97.04%), Postives = 199/203 (98.03%), Query Frame = 0


Query: 5   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY 64
           MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHG+NWGAADHTAADLY
Sbjct: 1   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGMNWGAADHTAADLY 60

Query: 65  --MNININMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQQRWAYVKVNMDGVIVG 124
             MNININMKHSNNNNGVVVV+QDYYCC  EEEEEAEEVVQSKQQRWAYVKVNMDGVIVG
Sbjct: 61  MNMNININMKHSNNNNGVVVVEQDYYCC--EEEEEAEEVVQSKQQRWAYVKVNMDGVIVG 120

Query: 125 RKVCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQ 184
           RKVCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQ
Sbjct: 121 RKVCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQ 180

Query: 185 FVEDVKRLRIARKDEGFVAIHQN 206
           FVEDVKRLRIARKDEGFVAIHQN
Sbjct: 181 FVEDVKRLRIARKDEGFVAIHQN 201

[Close]

BLAST of Carg01857-RA vs. ExPASy TrEMBL
Match: A0A6J1GZV1 (Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111458660 PE=3 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.4e-108
Identity = 197/203 (97.04%), Postives = 199/203 (98.03%), Query Frame = 0


Query: 5   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY 64
           MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY
Sbjct: 1   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY 60

Query: 65  MNININMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK 124
           MNININMKHSNNNNGVVVV+QDYYCC  EEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK
Sbjct: 61  MNININMKHSNNNNGVVVVEQDYYCC--EEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRK 120

Query: 125 VCIFQHGSYSSLALQLEDMF--GRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQ 184
           VCIFQHGSYS+LALQLEDMF  GRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQ
Sbjct: 121 VCIFQHGSYSNLALQLEDMFGSGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQ 180

Query: 185 FVEDVKRLRIARKDEGFVAIHQN 206
           FVEDVKRLRIARKDEGFVAIHQN
Sbjct: 181 FVEDVKRLRIARKDEGFVAIHQN 201

[Close]

BLAST of Carg01857-RA vs. ExPASy TrEMBL
Match: A0A6J1JFN3 (Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111483950 PE=3 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 4.4e-107
Identity = 197/205 (96.10%), Postives = 199/205 (97.07%), Query Frame = 0


Query: 5   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGINWGAADHTAADLY 64
           MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHG+NWGAADHTAADLY
Sbjct: 1   MEPNQFSVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGMNWGAADHTAADLY 60

Query: 65  --MNININMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQQRWAYVKVNMDGVIVG 124
             MNININMKHSNNNNGVVVV+QDYYCC  EEEEEAEEVVQSKQQRWAYVKVNMDGVIVG
Sbjct: 61  MNMNININMKHSNNNNGVVVVEQDYYCC--EEEEEAEEVVQSKQQRWAYVKVNMDGVIVG 120

Query: 125 RKVCIFQHGSYSSLALQLEDMF--GRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPW 184
           RKVCIFQHGSYSSLALQLEDMF  GRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPW
Sbjct: 121 RKVCIFQHGSYSSLALQLEDMFGSGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPW 180

Query: 185 KQFVEDVKRLRIARKDEGFVAIHQN 206
           KQFVEDVKRLRIARKDEGFVAIHQN
Sbjct: 181 KQFVEDVKRLRIARKDEGFVAIHQN 203

[Close]

BLAST of Carg01857-RA vs. ExPASy TrEMBL
Match: A0A5D3CD62 (Auxin-responsive protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001290 PE=3 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 2.3e-63
Identity = 132/198 (66.67%), Postives = 144/198 (72.73%), Query Frame = 0


Query: 11  SVYCEGKGENGSIDLGLSLRTLQPEVVGSIEGYGEEDEHGI-NWGAADHTAADLYMNINI 70
           SVYCEGKGENG IDLGLSLRTL            E+ E GI NWG  D            
Sbjct: 14  SVYCEGKGENGIIDLGLSLRTL------------EQPEMGIMNWGGQD------------ 73

Query: 71  NMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQ--QRWAYVKVNMDGVIVGRKVCI 130
                          QDYYCC ++EE+  ++VVQSK+  Q+WAYVKVNMDGVIVGRKVCI
Sbjct: 74  ---------------QDYYCCTDQEEDHTDQVVQSKEQHQKWAYVKVNMDGVIVGRKVCI 133

Query: 131 FQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFVEDV 190
           FQ+ SYS+LALQLEDMFGRQCESGL+LFEN+SEFSLFYKDGDENWRSVGDVPWKQFVE V
Sbjct: 134 FQNASYSTLALQLEDMFGRQCESGLRLFENDSEFSLFYKDGDENWRSVGDVPWKQFVEGV 172

Query: 191 KRLRIARKDEGFVAIHQN 206
           KRLRIARKDE FV IHQN
Sbjct: 194 KRLRIARKDEAFVVIHQN 172

[Close]

BLAST of Carg01857-RA vs. TAIR 10
Match: AT2G01200.2 (indole-3-acetic acid inducible 32 )

HSP 1 Score: 144.1 bits (362), Expect = 1.3e-34
Identity = 84/194 (43.30%), Postives = 116/194 (59.79%), Query Frame = 0


Query: 13  YCEGKGENGSIDLGLSLRTLQ------PEVVGSIEGYGEEDEHGINWGAADHTAADLYMN 72
           Y + K   G IDLGLSLRT+Q      P  +  ++GYGE     I+W    +        
Sbjct: 20  YSQTKKGGGVIDLGLSLRTIQHETYLPPARMIGLDGYGEL----IDWSQPSY-------- 79

Query: 73  ININMKHSNNNNGVVVVQQDYYCCAEEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRKVC 132
            N   +  + + G   + Q YY        E E      + ++AYVKVN+DG++VGRKVC
Sbjct: 80  -NSITQLKSEDTGHQRLAQGYY------NNEGE-----SRGKYAYVKVNLDGLVVGRKVC 139

Query: 133 IFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFVED 192
           +   G+Y++LALQL DMFG Q  SGL+LF+ ESEFSL Y+D +  WR+VGDVPWK+FVE 
Sbjct: 140 LVDQGAYATLALQLNDMFGMQTVSGLRLFQTESEFSLVYRDREGIWRNVGDVPWKEFVES 189

Query: 193 VKRLRIARKDEGFV 201
           V R+RIAR+++  +
Sbjct: 200 VDRMRIARRNDALL 189

[Close]

BLAST of Carg01857-RA vs. TAIR 10
Match: AT1G15050.1 (indole-3-acetic acid inducible 34 )

HSP 1 Score: 129.4 bits (324), Expect = 3.3e-30
Identity = 56/92 (60.87%), Postives = 74/92 (80.43%), Query Frame = 0


Query: 106 QQRWAYVKVNMDGVIVGRKVCIFQHGSYSSLALQLEDMFGRQCESGLKLFENESEFSLFY 165
           +++W YVKV MDG++VGRKVC+  HGSYS+LA QLEDMFG Q  SGL+LF+ ESEF L Y
Sbjct: 89  RRKWGYVKVTMDGLVVGRKVCVLDHGSYSTLAHQLEDMFGMQSVSGLRLFQMESEFCLVY 148

Query: 166 KDGDENWRSVGDVPWKQFVEDVKRLRIARKDE 198
           +D +  WR+ GDVPW +F+E V+RLRI R+++
Sbjct: 149 RDEEGLWRNAGDVPWNEFIESVERLRITRRND 180

[Close]

BLAST of Carg01857-RA vs. TAIR 10
Match: AT2G33310.1 (auxin-induced protein 13 )

HSP 1 Score: 89.0 bits (219), Expect = 4.9e-18
Identity = 49/116 (42.24%), Postives = 68/116 (58.62%), Query Frame = 0


Query: 92  EEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRKVCIFQHGSYSSLALQLEDMFGR----- 151
           ++E ++  + V  K Q   ++KVNMDGV +GRKV +  H SY +LA  LEDMF R     
Sbjct: 112 DDEPKDVTKKVNGKVQ-VGFIKVNMDGVAIGRKVDLNAHSSYENLAQTLEDMFFRTNPGT 171

Query: 152 -----QCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFVEDVKRLRIARKDE 198
                Q    L+L +  SEF L Y+D + +W  VGDVPW+ F+  VKRLR+ +  E
Sbjct: 172 VGLTSQFTKPLRLLDGSSEFVLTYEDKEGDWMLVGDVPWRMFINSVKRLRVMKTSE 226

[Close]

BLAST of Carg01857-RA vs. TAIR 10
Match: AT2G33310.2 (auxin-induced protein 13 )

HSP 1 Score: 89.0 bits (219), Expect = 4.9e-18
Identity = 49/116 (42.24%), Postives = 68/116 (58.62%), Query Frame = 0


Query: 92  EEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRKVCIFQHGSYSSLALQLEDMFGR----- 151
           ++E ++  + V  K Q   ++KVNMDGV +GRKV +  H SY +LA  LEDMF R     
Sbjct: 113 DDEPKDVTKKVNGKVQ-VGFIKVNMDGVAIGRKVDLNAHSSYENLAQTLEDMFFRTNPGT 172

Query: 152 -----QCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFVEDVKRLRIARKDE 198
                Q    L+L +  SEF L Y+D + +W  VGDVPW+ F+  VKRLR+ +  E
Sbjct: 173 VGLTSQFTKPLRLLDGSSEFVLTYEDKEGDWMLVGDVPWRMFINSVKRLRVMKTSE 227

[Close]

BLAST of Carg01857-RA vs. TAIR 10
Match: AT2G33310.3 (auxin-induced protein 13 )

HSP 1 Score: 89.0 bits (219), Expect = 4.9e-18
Identity = 49/116 (42.24%), Postives = 68/116 (58.62%), Query Frame = 0


Query: 92  EEEEEEAEEVVQSKQQRWAYVKVNMDGVIVGRKVCIFQHGSYSSLALQLEDMFGR----- 151
           ++E ++  + V  K Q   ++KVNMDGV +GRKV +  H SY +LA  LEDMF R     
Sbjct: 112 DDEPKDVTKKVNGKVQ-VGFIKVNMDGVAIGRKVDLNAHSSYENLAQTLEDMFFRTNPGT 171

Query: 152 -----QCESGLKLFENESEFSLFYKDGDENWRSVGDVPWKQFVEDVKRLRIARKDE 198
                Q    L+L +  SEF L Y+D + +W  VGDVPW+ F+  VKRLR+ +  E
Sbjct: 172 VGLTSQFTKPLRLLDGSSEFVLTYEDKEGDWMLVGDVPWRMFINSVKRLRVMKTSE 226

The following BLAST results are available for this feature:

BLAST of Carg01857-RA vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7032061.1	1.4e-115	100.00	Auxin-responsive protein IAA32, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]
KAG6601273.1	1.0e-110	99.00	Auxin-responsive protein IAA32, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022957191.1	8.7e-110	98.01	auxin-responsive protein IAA32-like isoform X2 [Cucurbita moschata]	[more]
XP_022957190.1	2.8e-108	97.04	auxin-responsive protein IAA32-like isoform X1 [Cucurbita moschata]	[more]
XP_022986212.1	2.8e-108	97.04	auxin-responsive protein IAA32-like isoform X2 [Cucurbita maxima]	[more]

BLAST of Carg01857-RA vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RYC6	1.8e-33	43.30	Auxin-responsive protein IAA32 OS=Arabidopsis thaliana OX=3702 GN=IAA32 PE=1 SV=...	[more]
Q9C5X0	4.6e-29	60.87	Auxin-responsive protein IAA34 OS=Arabidopsis thaliana OX=3702 GN=IAA34 PE=1 SV=...	[more]
A2XB18	3.1e-17	44.74	Auxin-responsive protein IAA10 OS=Oryza sativa subsp. indica OX=39946 GN=IAA10 P...	[more]
Q0DWF2	3.1e-17	44.74	Auxin-responsive protein IAA10 OS=Oryza sativa subsp. japonica OX=39947 GN=IAA10...	[more]
Q38831	6.9e-17	42.24	Auxin-responsive protein IAA13 OS=Arabidopsis thaliana OX=3702 GN=IAA13 PE=1 SV=...	[more]

BLAST of Carg01857-RA vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GYJ4	4.2e-110	98.01	Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111458660 PE=3 SV=1	[more]
A0A6J1J6Y8	1.4e-108	97.04	Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111483950 PE=3 SV=1	[more]
A0A6J1GZV1	1.4e-108	97.04	Auxin-responsive protein OS=Cucurbita moschata OX=3662 GN=LOC111458660 PE=3 SV=1	[more]
A0A6J1JFN3	4.4e-107	96.10	Auxin-responsive protein OS=Cucurbita maxima OX=3661 GN=LOC111483950 PE=3 SV=1	[more]
A0A5D3CD62	2.3e-63	66.67	Auxin-responsive protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]

BLAST of Carg01857-RA vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G01200.2	1.3e-34	43.30	indole-3-acetic acid inducible 32	[more]
AT1G15050.1	3.3e-30	60.87	indole-3-acetic acid inducible 34	[more]
AT2G33310.1	4.9e-18	42.24	auxin-induced protein 13	[more]
AT2G33310.2	4.9e-18	42.24	auxin-induced protein 13	[more]
AT2G33310.3	4.9e-18	42.24	auxin-induced protein 13	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.20.90		coord: 103..202 e-value: 4.8E-28 score: 99.1
None	No IPR available	PANTHER	PTHR31734:SF114	AUXIN-RESPONSIVE PROTEIN IAA34	coord: 9..198
None	No IPR available	SUPERFAMILY	54277	CAD & PB1 domains	coord: 105..192
IPR033389	AUX/IAA domain	PFAM	PF02309	AUX_IAA	coord: 93..197 e-value: 8.1E-24 score: 84.8
IPR003311	AUX/IAA protein	PANTHER	PTHR31734	AUXIN-RESPONSIVE PROTEIN IAA17	coord: 9..198
IPR000270	PB1 domain	PROSITE	PS51745	PB1	coord: 109..197 score: 19.707438

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Carg01857	Carg01857	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg01857-RA:1	Carg01857-RA:1	exon
Carg01857-RA:2	Carg01857-RA:2	exon
Carg01857-RA:3	Carg01857-RA:3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg01857-RA:cds	Carg01857-RA:cds	CDS
Carg01857-RA:cds	Carg01857-RA:cds_2	CDS
Carg01857-RA:cds	Carg01857-RA:cds_3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Carg01857-RA:three_prime_utr	Carg01857-RA:three_prime_utr	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Carg01857-RA	Carg01857-RA-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0009734	auxin-activated signaling pathway
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005515	protein binding