Homology
BLAST of CaUC11G218300.1 vs. NCBI nr
Match:
XP_038892964.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Benincasa hispida])
HSP 1 Score: 850.9 bits (2197), Expect = 5.5e-243
Identity = 416/497 (83.70%), Postives = 460/497 (92.56%), Query Frame = 0
Query: 1 MASQEEFLVSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHIS 60
+ASQE+F++ V+ LLLPL F+VFKL P SK+PPIPPGPKPWPILGNLLQIG+NAHIS
Sbjct: 9 VASQEDFVLCVI--LLLPLLFVVFKLISFPSSKKPPIPPGPKPWPILGNLLQIGKNAHIS 68
Query: 61 ITHLAQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECS 120
ITH AQTYGPLISLR GGRLLVVASSPAAAAAVLKTQDRLLS RYVFHMTPD AQH ECS
Sbjct: 69 ITHFAQTYGPLISLRLGGRLLVVASSPAAAAAVLKTQDRLLSNRYVFHMTPDLAQHHECS 128
Query: 121 LVFSPECNDRWRSLRSICKANLFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFV 180
LVFSPECND WRSLRSICKANLFTA+AI+SQ SLRRRKMNEM+E LLSKQGS+VEIRDFV
Sbjct: 129 LVFSPECNDHWRSLRSICKANLFTAKAIKSQASLRRRKMNEMIEFLLSKQGSVVEIRDFV 188
Query: 181 LTTVFNILSNFIFSRDLFDFDGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLR 240
LTTVFN LSNFIFSRDL DFDG+GFNG+KQ FNNLKNLGL PNLGDFYPFL NLD+QGLR
Sbjct: 189 LTTVFNALSNFIFSRDLIDFDGEGFNGLKQSFNNLKNLGLAPNLGDFYPFLRNLDVQGLR 248
Query: 241 NKCSLYSKQINSTFDILINERRKVSSPNSSSPRDFLDVLIQSQFTDDQISYLVIELLLAG 300
NKCS+YSKQINST DI+IN+RRK++S + S+P+DFLDV+IQSQFT+D I+YLV+ELLLAG
Sbjct: 249 NKCSIYSKQINSTLDIIINQRRKLASTDPSAPQDFLDVMIQSQFTNDHINYLVVELLLAG 308
Query: 301 TDTSTMTVEWTMAEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLH 360
TDTST+TVEWTMAE+LKNK+I+EKVREE+ E KQNSIDE+E+ KLKYLWKCVKETLRLH
Sbjct: 309 TDTSTITVEWTMAELLKNKNIIEKVREEVNTEMKQNSIDESEIYKLKYLWKCVKETLRLH 368
Query: 361 PPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVE 420
PPAPFLLPRIAP+DCELMGYSIPK++LIFVN+WGIGRDPSIWE+ LSFNP RFSD DHV+
Sbjct: 369 PPAPFLLPRIAPIDCELMGYSIPKESLIFVNVWGIGRDPSIWEESLSFNPMRFSDCDHVD 428
Query: 421 FKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDG-EDFSKVDMEGMLG 480
KGND+GYLPFGGGRRICPGFPMAT+ LPLILA LIHNFEWSLP+G EDFSK+DMEGMLG
Sbjct: 429 LKGNDFGYLPFGGGRRICPGFPMATVLLPLILANLIHNFEWSLPNGDEDFSKMDMEGMLG 488
Query: 481 VTLQKKQPLKLIPKKRF 497
VTL KKQPLKLIPKKRF
Sbjct: 489 VTLLKKQPLKLIPKKRF 503
BLAST of CaUC11G218300.1 vs. NCBI nr
Match:
XP_022159319.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Momordica charantia])
HSP 1 Score: 715.7 bits (1846), Expect = 2.8e-202
Identity = 355/497 (71.43%), Postives = 415/497 (83.50%), Query Frame = 0
Query: 2 ASQEEFLVSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISI 61
A +E + + I LLL FI K FRSP SKQ +PPGPKPWPILGNLLQIG +AH S+
Sbjct: 6 ADDQEQQLVLPILLLLASLFITLKYFRSPSSKQTKLPPGPKPWPILGNLLQIGEDAHASM 65
Query: 62 THLAQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSL 121
T AQ YGPLISLR GG+LLVVASSP AAA VLKTQDRLLS RY+F D A HRECS
Sbjct: 66 TRFAQAYGPLISLRLGGQLLVVASSPDAAAVVLKTQDRLLSARYIFQTVHDEALHRECSF 125
Query: 122 VFSPECNDRWRSLRSICKANLFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFVL 181
VFSPECNDRWR +RSIC+ANLFTA+AI+SQ +LRR KM+EM+E L+SKQGS+VEIRDFV
Sbjct: 126 VFSPECNDRWRKVRSICRANLFTAKAIDSQANLRRMKMDEMVEFLVSKQGSVVEIRDFVF 185
Query: 182 TTVFNILSNFIFSRDLFDFDGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRN 241
TTVFNILSNFIFSRDLFDF GDGFNGIK + LGLTPNL D+Y L NLD+QGLR
Sbjct: 186 TTVFNILSNFIFSRDLFDFVGDGFNGIKASLKKMVTLGLTPNLADYYSLLRNLDLQGLRK 245
Query: 242 KCSLYSKQINSTFDILINERRKVSSPNSSS--PRDFLDVLIQSQFTDDQISYLVIELLLA 301
K ++Y KQI+S + LI+ERRK+ NS + RDFLDVLIQSQF D+QI++L++E+ LA
Sbjct: 246 KATIYRKQIDSAWKELIDERRKIHLQNSPNFVARDFLDVLIQSQFNDEQINHLIVEIFLA 305
Query: 302 GTDTSTMTVEWTMAEVLKNKDIMEKVREEMKRE-TKQNSIDETEVCKLKYLWKCVKETLR 361
GTDT+TMTVEW MAE+LK +DIM KVREEMKRE Q+++DE+++ +L YLWKCVKETLR
Sbjct: 306 GTDTTTMTVEWAMAEMLKQRDIMAKVREEMKREINNQSTVDESKISQLHYLWKCVKETLR 365
Query: 362 LHPPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDH 421
LHPPAPFLLPR+AP DCELMGYSIPKDTLIFVN+WGIGRDPS+WED LSFNP+RF+D D
Sbjct: 366 LHPPAPFLLPRVAPTDCELMGYSIPKDTLIFVNVWGIGRDPSLWEDSLSFNPERFTDCD- 425
Query: 422 VEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGML 481
V+FKG DY YLPFGGGRRICPG PMA++Q+PLILATLIHNFE SLP+G+DFSK+D++G L
Sbjct: 426 VDFKGYDYRYLPFGGGRRICPGLPMASVQIPLILATLIHNFELSLPNGQDFSKLDLDGKL 485
Query: 482 GVTLQKKQPLKLIPKKR 496
GVTLQKK+PL+LI +KR
Sbjct: 486 GVTLQKKKPLQLILQKR 501
BLAST of CaUC11G218300.1 vs. NCBI nr
Match:
XP_022928791.1 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata])
HSP 1 Score: 676.4 bits (1744), Expect = 1.9e-190
Identity = 331/483 (68.53%), Postives = 392/483 (81.16%), Query Frame = 0
Query: 13 IFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLI 72
+ LLLPL FI+ + S SKQPP+PPGPKPWPILGNLLQIG+NAHIS+T + YGPLI
Sbjct: 9 LLLLLPLIFIILRHI-SSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 68
Query: 73 SLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDRWR 132
SL+ G + LVVASSPAAAAA+LKTQDRLLS RY+F MTPDR H CSL+FSPEC DRW+
Sbjct: 69 SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128
Query: 133 SLRSICKANLFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFVLTTVFNILSNFI 192
+LRSIC+A LFTARAIESQ SLRR+KMNEM+E L SKQGS+VEI++ V TVFN LSNFI
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188
Query: 193 FSRDLFDFDGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKCSLYSKQINS 252
FSRDL F GDGFNGIK PF N+ +LGL PNL DFYP L D+QGLR K ++Y Q++S
Sbjct: 189 FSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDS 248
Query: 253 TFDILINERRKVSSPNSSSPRDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWTM 312
+++ILI ERR + S S+ DFLDVLIQS FTD+QISYL++E+L AGTDT+T TVEW M
Sbjct: 249 SWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEWAM 308
Query: 313 AEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIAP 372
AE+LKN+DIM +VREE K+ +IDE+++ +L YLWKCVKETLRLHPPAPFLLPR AP
Sbjct: 309 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 368
Query: 373 MDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGNDYGYLPFG 432
CELMGYS+PK T+IFVN+WGIGRDPSIWED SF P+RF D + KG+DY +LPFG
Sbjct: 369 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCD-ADLKGHDYRFLPFG 428
Query: 433 GGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPLKLIP 492
GGRRICPG PMA +Q+PLILATLIHNFEW LP+ ED S +D+ G LGVTLQK++PLKL+
Sbjct: 429 GGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLLS 488
Query: 493 KKR 496
K R
Sbjct: 489 KNR 489
BLAST of CaUC11G218300.1 vs. NCBI nr
Match:
KAA0055170.1 (putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. makuwa])
HSP 1 Score: 674.1 bits (1738), Expect = 9.3e-190
Identity = 329/487 (67.56%), Postives = 396/487 (81.31%), Query Frame = 0
Query: 13 IFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLI 72
+ LLLP+ FI+ K +SP SK P+PPGP PWPILGNLLQIG+NAHISIT A YGPLI
Sbjct: 13 LLLLLPI-FIILKHIKSPSSKL-PLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLI 72
Query: 73 SLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDRWR 132
SL+ G + L+VASSPAAA AVLKTQDRLLS RY+F MTPDRA H + SLVFSPEC D+W+
Sbjct: 73 SLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQWK 132
Query: 133 SLRSICKANLFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFVLTTVFNILSNFI 192
+LRS+CK NLFTA+AIESQ LR +KM E+++ L SKQGS+VE++DFV T+VFN LSN I
Sbjct: 133 NLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTLSNLI 192
Query: 193 FSRDLFDFDGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKCSLYSKQINS 252
FS DL DF GDGF GIK PF + +LGLTPNL DFYP L D+QGL+NK +Y +++S
Sbjct: 193 FSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVDS 252
Query: 253 TFDILINERRKVSSPNSSSPRDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWTM 312
+ ILI ERR++ S+ DFLDVLIQS F D QI+ L+IELL AGTDT+T TVEW M
Sbjct: 253 LWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEWAM 312
Query: 313 AEVLKNKDIMEKVREEMKRETKQN-SIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIA 372
+E+LK+KDI+ KVREEMK+ TK+N IDE+ + +L+YLW+CVKETLRLHPPAPFLLPR+A
Sbjct: 313 SEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPRLA 372
Query: 373 PMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFS---DYDHVEFKGNDYGY 432
PMDCE+MGYSIPKD+++FVN+WGIGRDPS+WED +FNPQRF D+V+FKG DY Y
Sbjct: 373 PMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDYRY 432
Query: 433 LPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPL 492
LPFGGGRRICPG PMA +Q+PLILATLIHNFEWSLP+ ED SK+D+ G LGVTLQK + L
Sbjct: 433 LPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYETL 492
Query: 493 KLIPKKR 496
KLIPK+R
Sbjct: 493 KLIPKRR 497
BLAST of CaUC11G218300.1 vs. NCBI nr
Match:
XP_008467223.1 (PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo])
HSP 1 Score: 674.1 bits (1738), Expect = 9.3e-190
Identity = 329/487 (67.56%), Postives = 396/487 (81.31%), Query Frame = 0
Query: 13 IFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLI 72
+ LLLP+ FI+ K +SP SK P+PPGP PWPILGNLLQIG+NAHISIT A YGPLI
Sbjct: 13 LLLLLPI-FIILKHIKSPSSKL-PLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLI 72
Query: 73 SLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDRWR 132
SL+ G + L+VASSPAAA AVLKTQDRLLS RY+F MTPDRA H + SLVFSPEC D+W+
Sbjct: 73 SLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQWK 132
Query: 133 SLRSICKANLFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFVLTTVFNILSNFI 192
+LRS+CK NLFTA+AIESQ LR +KM E+++ L SKQGS+VE++DFV T+VFN LSN I
Sbjct: 133 NLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTLSNLI 192
Query: 193 FSRDLFDFDGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKCSLYSKQINS 252
FS DL DF GDGF GIK PF + +LGLTPNL DFYP L D+QGL+NK +Y +++S
Sbjct: 193 FSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVDS 252
Query: 253 TFDILINERRKVSSPNSSSPRDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWTM 312
+ ILI ERR++ S+ DFLDVLIQS F D QI+ L+IELL AGTDT+T TVEW M
Sbjct: 253 LWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEWAM 312
Query: 313 AEVLKNKDIMEKVREEMKRETKQN-SIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIA 372
+E+LK+KDI+ KVREEMK+ TK+N IDE+ + +L+YLW+CVKETLRLHPPAPFLLPR+A
Sbjct: 313 SEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPRLA 372
Query: 373 PMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFS---DYDHVEFKGNDYGY 432
PMDCE+MGYSIPKD+++FVN+WGIGRDPS+WED +FNPQRF D+V+FKG DY Y
Sbjct: 373 PMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDYRY 432
Query: 433 LPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPL 492
LPFGGGRRICPG PMA +Q+PLILATLIHNFEWSLP+ ED SK+D+ G LGVTLQK + L
Sbjct: 433 LPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYETL 492
Query: 493 KLIPKKR 496
KLIPK+R
Sbjct: 493 KLIPKRR 497
BLAST of CaUC11G218300.1 vs. ExPASy Swiss-Prot
Match:
Q9FXW4 (Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3442 GN=CYP80B2 PE=2 SV=1)
HSP 1 Score: 407.1 bits (1045), Expect = 2.8e-112
Identity = 217/489 (44.38%), Postives = 308/489 (62.99%), Query Frame = 0
Query: 9 VSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTY 68
+ VL ++ F++F F S+ +PPGP+P PI+GNLLQ+G H LAQ Y
Sbjct: 1 MEVLSIAIVSFSFLLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEFAKLAQKY 60
Query: 69 GPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECN 128
G L SL+ G + +VVASSPAAAA +LKT D++LSGRYVF + +H E S+V+S ECN
Sbjct: 61 GELFSLKLGSQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVK-EHVENSIVWS-ECN 120
Query: 129 DRWRSLRSICKANLFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFVLTTVFNIL 188
D W+ LR +C+ LFT + IESQ +R K EM++ L K+G +V+I + V T+ NI
Sbjct: 121 DNWKLLRKVCRTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIF 180
Query: 189 SNFIFSRDLFDFDG--DGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKCSLY 248
N IFS+D+FD + G +K+ L ++G + N D++P + LD+ G R +
Sbjct: 181 GNLIFSKDVFDLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEV 240
Query: 249 SKQINSTFDILINERRKVSSPNSSSPRDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTM 308
+QI + +++ ERR + S S DF+DVL+ + D +I+ L++EL AGT+TS
Sbjct: 241 LQQIYDVWGVMLKERR--GTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSAS 300
Query: 309 TVEWTMAEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAPFL 368
T+EW + E+ K ++ K+R E+ N++ E+++ L YL VKETLRLHPP P L
Sbjct: 301 TIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLL 360
Query: 369 LPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGNDY 428
LPR A C +M Y+IPK+ I VN W IGRDP W+D L+F P+RF D V++KGND+
Sbjct: 361 LPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSD-VDYKGNDF 420
Query: 429 GYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQ 488
+PFGGGRRICPG P+A+ LI+ATL+ NFEWSLP G S++ M+ G+TLQK
Sbjct: 421 ELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDP 480
Query: 489 PLKLIPKKR 496
PL ++ K R
Sbjct: 481 PLLIVLKAR 484
BLAST of CaUC11G218300.1 vs. ExPASy Swiss-Prot
Match:
O64900 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3467 GN=CYP80B2 PE=2 SV=1)
HSP 1 Score: 390.6 bits (1002), Expect = 2.7e-107
Identity = 205/477 (42.98%), Postives = 295/477 (61.84%), Query Frame = 0
Query: 22 IVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLISLRFGGRLL 81
I++ LF S K +PPGPKPWPI+GNLLQ+G H LAQTYG + +L+ G +
Sbjct: 16 ILYLLFGSSGHKN--LPPGPKPWPIVGNLLQLGEKPHAQFAELAQTYGDIFTLKMGTETV 75
Query: 82 VVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDRWRSLRSICKAN 141
VVAS+ +AA+ +LKT DR+LS RYVF + H E S+V+S +C + W++LR +C+
Sbjct: 76 VVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DCTETWKNLRKVCRTE 135
Query: 142 LFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFVLTTVFNILSNFIFSRDLFDF- 201
LFT + IESQ +R +K EM+E L+ KQG V+I + + T+ NI N IFS+++F+
Sbjct: 136 LFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELG 195
Query: 202 -DGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKCSLYSKQINSTFDILIN 261
G + K+ + LG + N D++P L D+ G R + + K I + + ++
Sbjct: 196 XPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQ 255
Query: 262 ERRKVSSPNS-SSPRDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWTMAEVLKN 321
ER+ + S DF+DV + S D QI+ L++EL AGT+TS T+EW M E+ KN
Sbjct: 256 ERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELTKN 315
Query: 322 KDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIAPMDCELM 381
I K+R E++ + S+ E++ L YL VKETLRLHPP P LLPR A C ++
Sbjct: 316 PKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTIL 375
Query: 382 GYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGNDYGYLPFGGGRRIC 441
Y+IPKD I VN WGIGRDP W D L+F+P+RF + V+F+GND+ +PFG GRRIC
Sbjct: 376 NYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLN-SSVDFRGNDFSLIPFGAGRRIC 435
Query: 442 PGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPLKLIPKKR 496
PG P+A + L++AT + N +W LP+G + +E G+TLQK+ PL ++PK R
Sbjct: 436 PGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPKSR 487
BLAST of CaUC11G218300.1 vs. ExPASy Swiss-Prot
Match:
Q9SP06 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum OX=3469 GN=CYP80B3 PE=1 SV=1)
HSP 1 Score: 389.8 bits (1000), Expect = 4.6e-107
Identity = 210/491 (42.77%), Postives = 304/491 (61.91%), Query Frame = 0
Query: 8 LVSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQT 67
LV+V+I L + +FR S +PPGPKPWPI+GNLLQ+G H LA+T
Sbjct: 2 LVAVVITTFL------YLIFRD--SSPKGLPPGPKPWPIVGNLLQLGEKPHSQFAQLAET 61
Query: 68 YGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPEC 127
YG L SL+ G +VVAS+P AA+ +LKT DR+LSGRYVF + +H E S+V+S EC
Sbjct: 62 YGDLFSLKLGSETVVVASTPLAASEILKTHDRVLSGRYVFQSFRVK-EHVENSIVWS-EC 121
Query: 128 NDRWRSLRSICKANLFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFVLTTVFNI 187
N+ W+ LR +C+ LFT + IESQ +R K EM+E L G+ V+I + V T+ NI
Sbjct: 122 NETWKKLRKVCRTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNI 181
Query: 188 LSNFIFSRDLFDFDGDGFNG---IKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKCS 247
N IFS+++F GD +G +K+ + LG + N D++PFL D+ G +
Sbjct: 182 FGNLIFSQNIFKL-GDESSGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVA 241
Query: 248 LYSKQINSTFDILINERRKVSSPNSSSPRDFLDVLIQSQFTDDQISYLVIELLLAGTDTS 307
+ I S + ++ ER+ N+S DF+++L+ S D QI+ L++E+ AGT+TS
Sbjct: 242 DCLQGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETS 301
Query: 308 TMTVEWTMAEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAP 367
T+EW ++E+ KN + +R E+ + + E+++ + YL VKETLRLHP P
Sbjct: 302 ASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATP 361
Query: 368 FLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGN 427
LLPR A C+++ Y+IPK+ I VN WGIGRDP W D L F+P+RF + ++FKGN
Sbjct: 362 LLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLN-SSIDFKGN 421
Query: 428 DYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQK 487
D+ +PFG GRRICPG P+AT + LI+++L+ NF+W P G D S++ ME G+TLQK
Sbjct: 422 DFELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQK 480
Query: 488 KQPLKLIPKKR 496
+ PL ++PK R
Sbjct: 482 EPPLYIVPKTR 480
BLAST of CaUC11G218300.1 vs. ExPASy Swiss-Prot
Match:
O64899 ((S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia californica OX=3467 GN=CYP80B1 PE=1 SV=1)
HSP 1 Score: 386.0 bits (990), Expect = 6.6e-106
Identity = 207/491 (42.16%), Postives = 299/491 (60.90%), Query Frame = 0
Query: 8 LVSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQT 67
L++V+I +L L F S +PPGPKPWPI+GNLLQ+G H LAQT
Sbjct: 7 LIAVIISSILYLLF--------GGSGHKNLPPGPKPWPIVGNLLQLGEKPHAQFAELAQT 66
Query: 68 YGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPEC 127
YG + +L+ G +VVAS+ +AA+ +LKT DR+LS RYVF + H E S+V+S +C
Sbjct: 67 YGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKG-HVENSIVWS-DC 126
Query: 128 NDRWRSLRSICKANLFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFVLTTVFNI 187
+ W++LR +C+ LFT + IESQ +R +K EM+E L+ KQG V+I + + T+ NI
Sbjct: 127 TETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNI 186
Query: 188 LSNFIFSRDLFDF--DGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKCSL 247
N IFS+++F+ G + K+ + LG + N D++P L D+ G R + +
Sbjct: 187 FGNLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAE 246
Query: 248 YSKQINSTFDILINERRKVSSPNS-SSPRDFLDVLIQSQFTDDQISYLVIELLLAGTDTS 307
K I + + ++ ER+ + S DF+DV + S D QI+ L++EL AGT+TS
Sbjct: 247 CLKGIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETS 306
Query: 308 TMTVEWTMAEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAP 367
T+EW M E+ KN I K+R E++ + S+ E++ L YL VKETLRLHPP P
Sbjct: 307 ASTIEWAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTP 366
Query: 368 FLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGN 427
LLPR A C ++ Y+IPKD I VN WGIGRDP W D L+F+P+RF + V+F+GN
Sbjct: 367 LLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLN-SSVDFRGN 426
Query: 428 DYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQK 487
D+ +PFG GRRICPG P+A + L++AT + N +W LP+G + +E G+TLQK
Sbjct: 427 DFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQK 486
Query: 488 KQPLKLIPKKR 496
+ PL ++PK R
Sbjct: 487 EPPLFIVPKSR 486
BLAST of CaUC11G218300.1 vs. ExPASy Swiss-Prot
Match:
A8CDR5 (Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1)
HSP 1 Score: 357.5 bits (916), Expect = 2.5e-97
Identity = 192/492 (39.02%), Postives = 306/492 (62.20%), Query Frame = 0
Query: 9 VSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNA----HISITHL 68
+ + +F L+P+ +VF L P K +PPGP PWP++GNL + N HI++ ++
Sbjct: 3 LQIALFSLIPV-ILVFILLLKP--KYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANM 62
Query: 69 AQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFS 128
A+T+GP++ L G + V+AS+ AA +LKT DR+ S R++ M+ H + SLV+S
Sbjct: 63 ARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHI-RMSFRLKHHIKYSLVWS 122
Query: 129 PECNDRWRSLRSICKANLFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFVLTTV 188
+C D W+ LR I + +F+ + +++Q +R +K+ E+++ L SK+G +V+I FV T+
Sbjct: 123 -DCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTL 182
Query: 189 FNILSNFIFSRDLFDF-DGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKC 248
NIL N +FS+D+F + D GI+ + +G PN+ +FYP L LD+QGL+ KC
Sbjct: 183 LNILGNVVFSKDVFVYGDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKC 242
Query: 249 SLYSKQINSTFDILINERRKVSSPNSSSPRDFLDVLIQSQFTDDQISYLVIELLLAGTDT 308
++ ++ + ER+ + + +D LDVL+ + F D QI+ L +E G++T
Sbjct: 243 DERFIRVMKMWEGTVKERK---ANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSET 302
Query: 309 STMTVEWTMAEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPA 368
S+ T+EW +AE++K+ M KVR+E+ ++I E+++ +L YL C+KE +RLHP A
Sbjct: 303 SSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHPAA 362
Query: 369 PFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKG 428
PFLLPR A CE+MGY+IPK++ + VN + IGRDP W+D +F P+RF + D V+F G
Sbjct: 363 PFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESD-VDFHG 422
Query: 429 NDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQ 488
Y ++PFG GRR C G P+AT +PLI+ +L+HN+++ LP G + M ML +TL
Sbjct: 423 AHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLA 482
Query: 489 KKQPLKLIPKKR 496
L ++PK R
Sbjct: 483 IDPSLCVVPKAR 485
BLAST of CaUC11G218300.1 vs. ExPASy TrEMBL
Match:
A0A6J1E237 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Momordica charantia OX=3673 GN=LOC111025729 PE=3 SV=1)
HSP 1 Score: 715.7 bits (1846), Expect = 1.3e-202
Identity = 355/497 (71.43%), Postives = 415/497 (83.50%), Query Frame = 0
Query: 2 ASQEEFLVSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISI 61
A +E + + I LLL FI K FRSP SKQ +PPGPKPWPILGNLLQIG +AH S+
Sbjct: 6 ADDQEQQLVLPILLLLASLFITLKYFRSPSSKQTKLPPGPKPWPILGNLLQIGEDAHASM 65
Query: 62 THLAQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSL 121
T AQ YGPLISLR GG+LLVVASSP AAA VLKTQDRLLS RY+F D A HRECS
Sbjct: 66 TRFAQAYGPLISLRLGGQLLVVASSPDAAAVVLKTQDRLLSARYIFQTVHDEALHRECSF 125
Query: 122 VFSPECNDRWRSLRSICKANLFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFVL 181
VFSPECNDRWR +RSIC+ANLFTA+AI+SQ +LRR KM+EM+E L+SKQGS+VEIRDFV
Sbjct: 126 VFSPECNDRWRKVRSICRANLFTAKAIDSQANLRRMKMDEMVEFLVSKQGSVVEIRDFVF 185
Query: 182 TTVFNILSNFIFSRDLFDFDGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRN 241
TTVFNILSNFIFSRDLFDF GDGFNGIK + LGLTPNL D+Y L NLD+QGLR
Sbjct: 186 TTVFNILSNFIFSRDLFDFVGDGFNGIKASLKKMVTLGLTPNLADYYSLLRNLDLQGLRK 245
Query: 242 KCSLYSKQINSTFDILINERRKVSSPNSSS--PRDFLDVLIQSQFTDDQISYLVIELLLA 301
K ++Y KQI+S + LI+ERRK+ NS + RDFLDVLIQSQF D+QI++L++E+ LA
Sbjct: 246 KATIYRKQIDSAWKELIDERRKIHLQNSPNFVARDFLDVLIQSQFNDEQINHLIVEIFLA 305
Query: 302 GTDTSTMTVEWTMAEVLKNKDIMEKVREEMKRE-TKQNSIDETEVCKLKYLWKCVKETLR 361
GTDT+TMTVEW MAE+LK +DIM KVREEMKRE Q+++DE+++ +L YLWKCVKETLR
Sbjct: 306 GTDTTTMTVEWAMAEMLKQRDIMAKVREEMKREINNQSTVDESKISQLHYLWKCVKETLR 365
Query: 362 LHPPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDH 421
LHPPAPFLLPR+AP DCELMGYSIPKDTLIFVN+WGIGRDPS+WED LSFNP+RF+D D
Sbjct: 366 LHPPAPFLLPRVAPTDCELMGYSIPKDTLIFVNVWGIGRDPSLWEDSLSFNPERFTDCD- 425
Query: 422 VEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGML 481
V+FKG DY YLPFGGGRRICPG PMA++Q+PLILATLIHNFE SLP+G+DFSK+D++G L
Sbjct: 426 VDFKGYDYRYLPFGGGRRICPGLPMASVQIPLILATLIHNFELSLPNGQDFSKLDLDGKL 485
Query: 482 GVTLQKKQPLKLIPKKR 496
GVTLQKK+PL+LI +KR
Sbjct: 486 GVTLQKKKPLQLILQKR 501
BLAST of CaUC11G218300.1 vs. ExPASy TrEMBL
Match:
A0A6J1ELA6 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata OX=3662 GN=LOC111435609 PE=3 SV=1)
HSP 1 Score: 676.4 bits (1744), Expect = 9.0e-191
Identity = 331/483 (68.53%), Postives = 392/483 (81.16%), Query Frame = 0
Query: 13 IFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLI 72
+ LLLPL FI+ + S SKQPP+PPGPKPWPILGNLLQIG+NAHIS+T + YGPLI
Sbjct: 9 LLLLLPLIFIILRHI-SSSSKQPPLPPGPKPWPILGNLLQIGQNAHISMTQFSHIYGPLI 68
Query: 73 SLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDRWR 132
SL+ G + LVVASSPAAAAA+LKTQDRLLS RY+F MTPDR H CSL+FSPEC DRW+
Sbjct: 69 SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128
Query: 133 SLRSICKANLFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFVLTTVFNILSNFI 192
+LRSIC+A LFTARAIESQ SLRR+KMNEM+E L SKQGS+VEI++ V TVFN LSNFI
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNEMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188
Query: 193 FSRDLFDFDGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKCSLYSKQINS 252
FSRDL F GDGFNGIK PF N+ +LGL PNL DFYP L D+QGLR K ++Y Q++S
Sbjct: 189 FSRDLVGFVGDGFNGIKGPFKNMIDLGLIPNLADFYPLLRCFDLQGLRKKAAIYKNQVDS 248
Query: 253 TFDILINERRKVSSPNSSSPRDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWTM 312
+++ILI ERR + S S+ DFLDVLIQS FTD+QISYL++E+L AGTDT+T TVEW M
Sbjct: 249 SWEILIKERRTIHSQGVSAASDFLDVLIQSGFTDEQISYLIVEVLSAGTDTTTTTVEWAM 308
Query: 313 AEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIAP 372
AE+LKN+DIM +VREE K+ +IDE+++ +L YLWKCVKETLRLHPPAPFLLPR AP
Sbjct: 309 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 368
Query: 373 MDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGNDYGYLPFG 432
CELMGYS+PK T+IFVN+WGIGRDPSIWED SF P+RF D + KG+DY +LPFG
Sbjct: 369 EACELMGYSVPKHTMIFVNVWGIGRDPSIWEDPQSFKPERFDGCD-ADLKGHDYRFLPFG 428
Query: 433 GGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPLKLIP 492
GGRRICPG PMA +Q+PLILATLIHNFEW LP+ ED S +D+ G LGVTLQK++PLKL+
Sbjct: 429 GGRRICPGLPMAAVQVPLILATLIHNFEWCLPNDEDASTLDLNGRLGVTLQKEEPLKLLS 488
Query: 493 KKR 496
K R
Sbjct: 489 KNR 489
BLAST of CaUC11G218300.1 vs. ExPASy TrEMBL
Match:
A0A5A7UIU3 (Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G001030 PE=3 SV=1)
HSP 1 Score: 674.1 bits (1738), Expect = 4.5e-190
Identity = 329/487 (67.56%), Postives = 396/487 (81.31%), Query Frame = 0
Query: 13 IFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLI 72
+ LLLP+ FI+ K +SP SK P+PPGP PWPILGNLLQIG+NAHISIT A YGPLI
Sbjct: 13 LLLLLPI-FIILKHIKSPSSKL-PLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLI 72
Query: 73 SLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDRWR 132
SL+ G + L+VASSPAAA AVLKTQDRLLS RY+F MTPDRA H + SLVFSPEC D+W+
Sbjct: 73 SLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQWK 132
Query: 133 SLRSICKANLFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFVLTTVFNILSNFI 192
+LRS+CK NLFTA+AIESQ LR +KM E+++ L SKQGS+VE++DFV T+VFN LSN I
Sbjct: 133 NLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTLSNLI 192
Query: 193 FSRDLFDFDGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKCSLYSKQINS 252
FS DL DF GDGF GIK PF + +LGLTPNL DFYP L D+QGL+NK +Y +++S
Sbjct: 193 FSMDLIDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVDS 252
Query: 253 TFDILINERRKVSSPNSSSPRDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWTM 312
+ ILI ERR++ S+ DFLDVLIQS F D QI+ L+IELL AGTDT+T TVEW M
Sbjct: 253 LWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEWAM 312
Query: 313 AEVLKNKDIMEKVREEMKRETKQN-SIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIA 372
+E+LK+KDI+ KVREEMK+ TK+N IDE+ + +L+YLW+CVKETLRLHPPAPFLLPR+A
Sbjct: 313 SEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPRLA 372
Query: 373 PMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFS---DYDHVEFKGNDYGY 432
PMDCE+MGYSIPKD+++FVN+WGIGRDPS+WED +FNPQRF D+V+FKG DY Y
Sbjct: 373 PMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDYRY 432
Query: 433 LPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPL 492
LPFGGGRRICPG PMA +Q+PLILATLIHNFEWSLP+ ED SK+D+ G LGVTLQK + L
Sbjct: 433 LPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYETL 492
Query: 493 KLIPKKR 496
KLIPK+R
Sbjct: 493 KLIPKRR 497
BLAST of CaUC11G218300.1 vs. ExPASy TrEMBL
Match:
A0A1S3CT14 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656 GN=LOC103504626 PE=3 SV=1)
HSP 1 Score: 674.1 bits (1738), Expect = 4.5e-190
Identity = 329/487 (67.56%), Postives = 396/487 (81.31%), Query Frame = 0
Query: 13 IFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLI 72
+ LLLP+ FI+ K +SP SK P+PPGP PWPILGNLLQIG+NAHISIT A YGPLI
Sbjct: 13 LLLLLPI-FIILKHIKSPSSKL-PLPPGPNPWPILGNLLQIGQNAHISITQFANIYGPLI 72
Query: 73 SLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDRWR 132
SL+ G + L+VASSPAAA AVLKTQDRLLS RY+F MTPDRA H + SLVFSPEC D+W+
Sbjct: 73 SLKLGAQRLIVASSPAAATAVLKTQDRLLSARYIFQMTPDRALHDQHSLVFSPECGDQWK 132
Query: 133 SLRSICKANLFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFVLTTVFNILSNFI 192
+LRS+CK NLFTA+AIESQ LR +KM E+++ L SKQGS+VE++DFV T+VFN LSN I
Sbjct: 133 NLRSLCKVNLFTAKAIESQAILREKKMKELVKFLESKQGSVVEVKDFVFTSVFNTLSNLI 192
Query: 193 FSRDLFDFDGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKCSLYSKQINS 252
FS DL DF GDGF GIK PF + +LGLTPNL DFYP L D+QGL+NK +Y +++S
Sbjct: 193 FSMDLLDFVGDGFKGIKGPFKKMMDLGLTPNLADFYPLLRRFDLQGLKNKADIYKNEVDS 252
Query: 253 TFDILINERRKVSSPNSSSPRDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWTM 312
+ ILI ERR++ S+ DFLDVLIQS F D QI+ L+IELL AGTDT+T TVEW M
Sbjct: 253 LWGILIKERREIHCQQGSASNDFLDVLIQSGFDDQQINALIIELLSAGTDTTTTTVEWAM 312
Query: 313 AEVLKNKDIMEKVREEMKRETKQN-SIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIA 372
+E+LK+KDI+ KVREEMK+ TK+N IDE+ + +L+YLW+CVKETLRLHPPAPFLLPR+A
Sbjct: 313 SEILKDKDILNKVREEMKKVTKENRPIDESNISELQYLWRCVKETLRLHPPAPFLLPRLA 372
Query: 373 PMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFS---DYDHVEFKGNDYGY 432
PMDCE+MGYSIPKD+++FVN+WGIGRDPS+WED +FNPQRF D+V+FKG DY Y
Sbjct: 373 PMDCEVMGYSIPKDSMVFVNVWGIGRDPSVWEDSQTFNPQRFDVGCGNDNVDFKGYDYRY 432
Query: 433 LPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPL 492
LPFGGGRRICPG PMA +Q+PLILATLIHNFEWSLP+ ED SK+D+ G LGVTLQK + L
Sbjct: 433 LPFGGGRRICPGLPMAIVQVPLILATLIHNFEWSLPNDEDLSKLDLNGRLGVTLQKYETL 492
Query: 493 KLIPKKR 496
KLIPK+R
Sbjct: 493 KLIPKRR 497
BLAST of CaUC11G218300.1 vs. ExPASy TrEMBL
Match:
A0A6J1I4V7 (probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=3661 GN=LOC111469167 PE=3 SV=1)
HSP 1 Score: 673.7 bits (1737), Expect = 5.9e-190
Identity = 328/483 (67.91%), Postives = 394/483 (81.57%), Query Frame = 0
Query: 13 IFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGPLI 72
+ LLLPL F + + S SKQPP+PPGP PWPILGNLLQIG+NAHIS+T + YGPLI
Sbjct: 9 LLLLLPLIFTILRHICS-SSKQPPLPPGPNPWPILGNLLQIGQNAHISMTQFSHFYGPLI 68
Query: 73 SLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDRWR 132
SL+ G + LVVASSPAAAAA+LKTQDRLLS RY+F MTPDR H CSL+FSPEC DRW+
Sbjct: 69 SLKLGAQRLVVASSPAAAAAILKTQDRLLSARYIFQMTPDRPLHDNCSLLFSPECGDRWK 128
Query: 133 SLRSICKANLFTARAIESQGSLRRRKMNEMMELLLSKQGSIVEIRDFVLTTVFNILSNFI 192
+LRSIC+A LFTARAIESQ SLRR+KMN+M+E L SKQGS+VEI++ V TVFN LSNFI
Sbjct: 129 NLRSICRAKLFTARAIESQASLRRKKMNDMVEFLQSKQGSVVEIKELVFITVFNTLSNFI 188
Query: 193 FSRDLFDFDGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKCSLYSKQINS 252
FSRDL F GDGFNGIK F N+ +LGL PNL DFYP L + D+QGLR K ++Y Q++S
Sbjct: 189 FSRDLVGFVGDGFNGIKGSFKNIIDLGLIPNLADFYPLLRSFDLQGLRKKAAIYKNQVDS 248
Query: 253 TFDILINERRKVSSPNSSSPRDFLDVLIQSQFTDDQISYLVIELLLAGTDTSTMTVEWTM 312
+++ILI ERR + S + S+ DFLDVLI+S FTD+QISYL++E+L AGTDT+T TVEW M
Sbjct: 249 SWEILIKERRTIHSRDVSAASDFLDVLIESGFTDEQISYLIVEVLSAGTDTTTTTVEWAM 308
Query: 313 AEVLKNKDIMEKVREEMKRETKQNSIDETEVCKLKYLWKCVKETLRLHPPAPFLLPRIAP 372
AE+LKN+DIM +VREE K+ +IDE+++ +L YLWKCVKETLRLHPPAPFLLPR AP
Sbjct: 309 AELLKNRDIMNRVREETKKAINGGAIDESKLSELHYLWKCVKETLRLHPPAPFLLPRYAP 368
Query: 373 MDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRFSDYDHVEFKGNDYGYLPFG 432
CELMGYS+PKDT+IFVN+WGIGRDPSIWED SF P+RF +D + KG+DY +LPFG
Sbjct: 369 EACELMGYSVPKDTMIFVNVWGIGRDPSIWEDPGSFKPERFDGFD-ADLKGHDYRFLPFG 428
Query: 433 GGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDMEGMLGVTLQKKQPLKLIP 492
GGRRICPG PMA +Q+PLILATLIHNFEW LP+ ED S +D+ G LGVTLQK++PLKL+
Sbjct: 429 GGRRICPGLPMAAVQVPLILATLIHNFEWYLPNDEDASTLDLNGRLGVTLQKEEPLKLLS 488
Query: 493 KKR 496
KKR
Sbjct: 489 KKR 489
BLAST of CaUC11G218300.1 vs. TAIR 10
Match:
AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )
HSP 1 Score: 342.4 bits (877), Expect = 6.0e-94
Identity = 194/503 (38.57%), Postives = 294/503 (58.45%), Query Frame = 0
Query: 13 IFLLLPLFFIVFKLFRSPCS--KQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYGP 72
+F + FFI+F P S K P PPGP PI+GN+ +GRN H S L++TYGP
Sbjct: 12 LFCFVLSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHSFADLSKTYGP 71
Query: 73 LISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRA----QHRECSLVFSPE 132
++SL+FG VV +SP AA VL+T D++LS R TP + H + S+V+ P
Sbjct: 72 IMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSR-----TPTNSIRSINHDKVSVVWLPP 131
Query: 133 CNDRWRSLRSICKANLFTARAIESQGSLRRRKMNEMMELL--LSKQGSIVEIRDFVLTTV 192
+ RWR LR + LF+ + IE+ +LR K+ E++ + S++ V+I T
Sbjct: 132 SSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRATFITA 191
Query: 193 FNILSNFIFSRDLFDFDGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKCS 252
NI+SN +FS DL ++D + + + P+ +F+PFL LD+QG R
Sbjct: 192 LNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDLQGNRKTLK 251
Query: 253 LYSKQINSTFDILINER---RKVSSPNSSS--PRDFLDVLI------QSQFTDDQISYLV 312
S+++ F I+ + + + NS RDF+DVL+ +++ + I +L+
Sbjct: 252 ACSERLFKVFRGFIDAKLAEKSLRDTNSKDVRERDFVDVLLDLTEGDEAELNTNDIVHLL 311
Query: 313 IELLLAGTDTSTMTVEWTMAEVLKNKDIMEKVREEMKRETKQNS-IDETEVCKLKYLWKC 372
++L AGTDT++ TVEW MAE+L+N + M K + E+ Q ++E+++ L YL
Sbjct: 312 LDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVEESDISALPYLQAV 371
Query: 373 VKETLRLHPPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQR 432
VKET RLHP AP L+PR A D E++G+ +PKDT +FVN+W IGRDP++WE+ F P+R
Sbjct: 372 VKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDPNVWENSSRFKPER 431
Query: 433 FSDYDHVEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKV 492
F D ++ +G DY PFG GRRICPG P+A +PL+LA+L+++F+W LP+G +
Sbjct: 432 FLGKD-IDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLLYSFDWKLPNGVGSEDL 491
Query: 493 DMEGMLGVTLQKKQPLKLIPKKR 496
DM+ G+TL K PL +P K+
Sbjct: 492 DMDETFGLTLHKTNPLHAVPVKK 508
BLAST of CaUC11G218300.1 vs. TAIR 10
Match:
AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4 )
HSP 1 Score: 337.0 bits (863), Expect = 2.5e-92
Identity = 193/502 (38.45%), Postives = 293/502 (58.37%), Query Frame = 0
Query: 12 LIFLLLPLFFIVFKLFRSPCS--KQPPIPPGPKPWPILGNLLQIGRNAHISITHLAQTYG 71
L+F + F++ RS S + +PPGP PI+GN+ Q+G+N H S LA+ YG
Sbjct: 11 LLFCFISSCFLISTTARSRRSSGRAATLPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYG 70
Query: 72 PLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQ---HRECSLVFSPE 131
P++SL+FG VV +SP AA VL+T D++LSGR + D + H E S+++ P
Sbjct: 71 PIMSLKFGCLNSVVITSPEAAREVLRTHDQILSGR----KSNDSIRCFGHEEVSVIWLPP 130
Query: 132 CNDRWRSLRSICKANLFTARAIESQGSLRRRKMNEMMELL--LSKQGSIVEIRDFVLTTV 191
+ RWR LR + +F+ + E+ +LR +K+ E++ + S++ V+I TTV
Sbjct: 131 SSARWRMLRKLSVTLMFSPQRTEATKALRMKKVQELVSFMNESSERKEAVDISRASYTTV 190
Query: 192 FNILSNFIFSRDLFDFDGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKCS 251
NI+SN +FS DL +D N + P+ +++PF+ LD+QG R
Sbjct: 191 LNIISNILFSVDLGSYDSKKSNEFQDTVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMR 250
Query: 252 LYSKQINSTF----DILINERRKVSSPNSSSPRDFLDVLI------QSQFTDDQISYLVI 311
++++ F D I E+ + S RDFLD L+ +++ ++ I +L++
Sbjct: 251 GLTERLFRVFRGFMDAKIAEKSLGNYSKDVSNRDFLDSLLILNEGDEAELDNNDIEHLLL 310
Query: 312 ELLLAGTDTSTMTVEWTMAEVLKNKDIMEKVREEMKRETKQNS-IDETEVCKLKYLWKCV 371
++ AGTDTS+ T+EW MAE+L+N M K + EM R QNS + E+++ L YL V
Sbjct: 311 DMFTAGTDTSSSTLEWAMAELLRNPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVV 370
Query: 372 KETLRLHPPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRF 431
KET RLHP AP L+PR A D E++G+ +PKDT + VN+W IGRDPS+WE+ F P+RF
Sbjct: 371 KETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERF 430
Query: 432 SDYDHVEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVD 491
D ++ KG DY PFGGGRRICPG P+A + L+LA+L+++F+W LP+G +D
Sbjct: 431 MGKD-IDVKGRDYELTPFGGGRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLD 490
Query: 492 MEGMLGVTLQKKQPLKLIPKKR 496
M+ G+TL + L IP K+
Sbjct: 491 MDETFGITLHRTNTLYAIPVKK 507
BLAST of CaUC11G218300.1 vs. TAIR 10
Match:
AT1G33720.1 (cytochrome P450, family 76, subfamily C, polypeptide 6 )
HSP 1 Score: 334.7 bits (857), Expect = 1.2e-91
Identity = 197/508 (38.78%), Postives = 291/508 (57.28%), Query Frame = 0
Query: 1 MASQEEFLVSVLIFLLLPLFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQIGRNAHIS 60
++ Q FL+ I L LFF + RSPC PPGP PI+GN+ +G+N H S
Sbjct: 4 ISGQPLFLIFCFILSCL-LFFTTARSRRSPCQLSKS-PPGPPRLPIIGNIHLVGKNPHHS 63
Query: 61 ITHLAQTYGPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECS 120
T L++TYGP++SL+ G VV +S A VLKT D++LSGRY+ T H E S
Sbjct: 64 FTDLSKTYGPVMSLKLGCLNSVVIASRDAVREVLKTHDQILSGRYISEATKSN-NHHEFS 123
Query: 121 LVFSPECNDRWRSLRSICKANLFTARAIESQGSLRRRKMNEMMELLLS--KQGSIVEIRD 180
+ + + R+R LR + LF+ + I++ +LR +K+ E++ L ++ V+I
Sbjct: 124 VGWIHPSSSRFRMLRKLSATQLFSPQCIQATKALRMKKVQELVNFLSESCEREEAVDISH 183
Query: 181 FVLTTVFNILSNFIFSRDLFDFDGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQG 240
T NI+SN +FS +L +D + ++ + P+L +F+PF+ LD+QG
Sbjct: 184 VSFVTALNIISNILFSVNLGSYDSKNSSAFQEMVIGYQESIGNPDLANFFPFMRFLDLQG 243
Query: 241 LRNKCSLYSKQINSTF----DILINERRKVSSPNSSSPRDFLDVLIQSQFTD------DQ 300
K S ++ F D I E+ S S +DFLDVLI Q D D+
Sbjct: 244 NSKKMRESSGRLLQVFREFYDARIVEKSSRSVEKDVSSKDFLDVLIDLQQGDETEINIDE 303
Query: 301 ISYLVIELLLAGTDTSTMTVEWTMAEVLKNKDIMEKVREEMKRETKQN-SIDETEVCKLK 360
I +L++++ +AGTDT++ TVEW MAE+L N M KV++E+ QN E+++ KL
Sbjct: 304 IEHLLLDMFVAGTDTNSSTVEWAMAELLGNPKTMTKVQDEINHVIGQNGDFQESDISKLP 363
Query: 361 YLWKCVKETLRLHPPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLS 420
YL VKET RLHP APFLL R A + E++G+++ KD+ + VN+W IGRDP +WE+
Sbjct: 364 YLKAVVKETFRLHPAAPFLLQRKAETNVEILGFTVLKDSQVLVNVWAIGRDPLVWENPTH 423
Query: 421 FNPQRFSDYDHVEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGE 480
F P+RF + ++ KG DY PFG GRRICPG P+A + L+LA+L++ FEW LP+G
Sbjct: 424 FEPERFLGKE-IDVKGTDYELTPFGAGRRICPGLPLAMKTVHLMLASLLYTFEWKLPNGV 483
Query: 481 DFSKVDMEGMLGVTLQKKQPLKLIPKKR 496
+DME G+T+ K PL +P K+
Sbjct: 484 GSEDLDMEETFGLTVHKTNPLLAVPLKK 507
BLAST of CaUC11G218300.1 vs. TAIR 10
Match:
AT2G45560.1 (cytochrome P450, family 76, subfamily C, polypeptide 1 )
HSP 1 Score: 325.5 bits (833), Expect = 7.5e-89
Identity = 186/504 (36.90%), Postives = 291/504 (57.74%), Query Frame = 0
Query: 11 VLIFLLLPLFFIVFKLFRSPCSKQ--PPIPPGPKPWPILGNLLQIGRNAHISITHLAQTY 70
+L+F + F++F RS + +PPGP PI+GN+ +G++ H S L++TY
Sbjct: 10 LLLFCFILSCFLIFTTTRSGRISRGATALPPGPPRLPIIGNIHLVGKHPHRSFAELSKTY 69
Query: 71 GPLISLRFGGRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRA----QHRECSLVFS 130
GP++SL+ G VV +SP AA VL+T D++LS R +P A H++ SLV+
Sbjct: 70 GPVMSLKLGSLNTVVIASPEAAREVLRTHDQILSAR-----SPTNAVRSINHQDASLVWL 129
Query: 131 PECNDRWRSLRSICKANLFTARAIESQGSLRRRKMNEMMELL--LSKQGSIVEIRDFVLT 190
P + RWR LR + L + + IE+ +LR K+ E++ + S + V+I
Sbjct: 130 PSSSARWRLLRRLSVTQLLSPQRIEATKALRMNKVKELVSFISESSDREESVDISRVAFI 189
Query: 191 TVFNILSNFIFSRDLFDFDGD-GFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRN 250
T NI+SN +FS DL ++ NG++ ++ + TP+ +++PFL LD+QG
Sbjct: 190 TTLNIISNILFSVDLGSYNAKASINGVQDTVISVMDAAGTPDAANYFPFLRFLDLQGNVK 249
Query: 251 KCSLYSKQINSTF----DILINERRKVSSPNSSSPRDFLDVLI-----QSQFTDDQISYL 310
+ ++++ F D I E+ ++P S DF+D L+ +S+ + I +L
Sbjct: 250 TFKVCTERLVRVFRGFIDAKIAEKSSQNNPKDVSKNDFVDNLLDYKGDESELSISDIEHL 309
Query: 311 VIELLLAGTDTSTMTVEWTMAEVLKNKDIMEKVREEMKRETKQNSI-DETEVCKLKYLWK 370
++++ AGTDTS+ T+EW M E+LKN M K + E+ QN I +E+++ KL YL
Sbjct: 310 LLDMFTAGTDTSSSTLEWAMTELLKNPKTMAKAQAEIDCVIGQNGIVEESDISKLPYLQA 369
Query: 371 CVKETLRLHPPAPFLLPRIAPMDCELMGYSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQ 430
VKET RLH P P L+PR A D E++G+ + KDT + VN+W IGRDPS+W++ F P+
Sbjct: 370 VVKETFRLHTPVPLLIPRKAESDAEILGFMVLKDTQVLVNVWAIGRDPSVWDNPSQFEPE 429
Query: 431 RFSDYDHVEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSK 490
RF D ++ +G DY PFG GRRICPG P+A + L+LA+L+++F+W LP G
Sbjct: 430 RFLGKD-MDVRGRDYELTPFGAGRRICPGMPLAMKTVSLMLASLLYSFDWKLPKGVLSED 489
Query: 491 VDMEGMLGVTLQKKQPLKLIPKKR 496
+DM+ G+TL K PL +P K+
Sbjct: 490 LDMDETFGLTLHKTNPLHAVPVKK 507
BLAST of CaUC11G218300.1 vs. TAIR 10
Match:
AT3G52970.1 (cytochrome P450, family 76, subfamily G, polypeptide 1 )
HSP 1 Score: 311.2 bits (796), Expect = 1.5e-84
Identity = 172/497 (34.61%), Postives = 276/497 (55.53%), Query Frame = 0
Query: 19 LFFIVFKLFRSPCSKQPPIPPGPKPWPILGNLLQI-GRNAHISITHLAQTYGPLISLRFG 78
L ++ + C + +PPGP PWP++GN+ Q+ G H S+T L++ +GP+++LR G
Sbjct: 20 LIYVTCLFYTKRCRTR--LPPGPNPWPVIGNIFQLAGLPPHDSLTKLSRRHGPIMTLRIG 79
Query: 79 GRLLVVASSPAAAAAVLKTQDRLLSGRYVFHMTPDRAQHRECSLVFSPECNDRWRSLRSI 138
L VV SS A + K D L+GR ++ + + SL+ + + WR LR +
Sbjct: 80 SMLTVVISSSEVAREIFKKHDAALAGRKIYEAMKG-GKSSDGSLI-TAQYGAYWRMLRRL 139
Query: 139 CKANLFTARAIESQGSLRRRKMNEMMELLLSKQG-----SIVEIRDFVLTTVFNILSNFI 198
C F R +++ +R R +++M+ + ++G +++ + FN++ N +
Sbjct: 140 CTTQFFVTRRLDAMSDVRSRCVDQMLRFV--EEGGQNGTKTIDVGRYFFLMAFNLIGNLM 199
Query: 199 FSRDLFDFDGDGFNGIKQPFNNLKNLGLTPNLGDFYPFLTNLDMQGLRNKCSLYSKQINS 258
FSRDL D D + + PN+ DF+P L LD QG+R K + ++
Sbjct: 200 FSRDLLDPDSKRGSEFFYHTGKVMEFAGKPNVADFFPLLRFLDPQGIRRKTQFHVEKAFE 259
Query: 259 TFDILINERRKVSSPNSS--SPRDFLDVLIQ-----------SQFTDDQISYLVIELLLA 318
I ER +V S +D+LDVL++ S F+ I+ +V E+ A
Sbjct: 260 IAGEFIRERTEVREREKSDEKTKDYLDVLLEFRGGDGVDEEPSSFSSRDINVIVFEMFTA 319
Query: 319 GTDTSTMTVEWTMAEVLKNKDIMEKVREEMKRETKQNS--IDETEVCKLKYLWKCVKETL 378
GTDT+T T+EW +AE+L N + K++ E++ K ++ + E ++ L YL + ETL
Sbjct: 320 GTDTTTSTLEWALAELLHNPRTLTKLQTELRTYFKSSNQKLQEEDLPNLPYLSAVIMETL 379
Query: 379 RLHPPAPFLLPRIAPMDCELMG-YSIPKDTLIFVNIWGIGRDPSIWEDCLSFNPQRF-SD 438
RLHPP PFL+P A C + Y+IPK+T + VN+W IGRDP W D + F P+RF SD
Sbjct: 380 RLHPPLPFLVPHKAMSTCHIFDQYTIPKETQVLVNVWAIGRDPKTWIDPIMFKPERFISD 439
Query: 439 YDHVEFKGNDYGYLPFGGGRRICPGFPMATLQLPLILATLIHNFEWSLPDGEDFSKVDME 493
D +FKG DY +LPFG GRR+CP P+A+ LPL + +++ +F+W+L +G + ++DM
Sbjct: 440 PDARDFKGQDYEFLPFGSGRRMCPALPLASRVLPLAIGSMVRSFDWALENGLNAEEMDMG 499
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038892964.1 | 5.5e-243 | 83.70 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Benincasa hispida] | [more] |
| XP_022159319.1 | 2.8e-202 | 71.43 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Momordica charantia] | [more] |
| XP_022928791.1 | 1.9e-190 | 68.53 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucurbita moschata] | [more] |
| KAA0055170.1 | 9.3e-190 | 67.56 | putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis melo var. maku... | [more] |
| XP_008467223.1 | 9.3e-190 | 67.56 | PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 [Cucumis mel... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q9FXW4 | 2.8e-112 | 44.38 | Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Coptis japonica OX=3... | [more] |
| O64900 | 2.7e-107 | 42.98 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Eschscholzia californica OX=3... | [more] |
| Q9SP06 | 4.6e-107 | 42.77 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Papaver somniferum... | [more] |
| O64899 | 6.6e-106 | 42.16 | (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1 (Fragment) OS=Eschscholzia calif... | [more] |
| A8CDR5 | 2.5e-97 | 39.02 | Corytuberine synthase OS=Coptis japonica OX=3442 GN=CYP80G2 PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1E237 | 1.3e-202 | 71.43 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Momordica charantia ... | [more] |
| A0A6J1ELA6 | 9.0e-191 | 68.53 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita moschata O... | [more] |
| A0A5A7UIU3 | 4.5e-190 | 67.56 | Putative (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo var. ma... | [more] |
| A0A1S3CT14 | 4.5e-190 | 67.56 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucumis melo OX=3656... | [more] |
| A0A6J1I4V7 | 5.9e-190 | 67.91 | probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 OS=Cucurbita maxima OX=... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT2G45570.1 | 6.0e-94 | 38.57 | cytochrome P450, family 76, subfamily C, polypeptide 2 | [more] |
| AT2G45550.1 | 2.5e-92 | 38.45 | cytochrome P450, family 76, subfamily C, polypeptide 4 | [more] |
| AT1G33720.1 | 1.2e-91 | 38.78 | cytochrome P450, family 76, subfamily C, polypeptide 6 | [more] |
| AT2G45560.1 | 7.5e-89 | 36.90 | cytochrome P450, family 76, subfamily C, polypeptide 1 | [more] |
| AT3G52970.1 | 1.5e-84 | 34.61 | cytochrome P450, family 76, subfamily G, polypeptide 1 | [more] |
