CSPI07G16700.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI07G16700.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Methyl-CpG-binding domain-containing protein 4
Location	Chr7: 15268773 .. 15270674 (+)
Sequence length	935
RNA-Seq Expression	CSPI07G16700.1
Synteny	CSPI07G16700.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGGAAAGATTAATAAATTTTTCTTTAAAGCACAATTTACTTTTAAGATTTGTTATTAGAGATCACGCCATAGACGAGGCGAAATGGCCGCCGCCGCCGACAGACCTCCCAAAACGCCGACAAAGGTACGCCGGAAACTCTATTCTCTTCCTCCAATTTATTCTTTATATCCTACATGGTTTCCTCTTTATCGGATTCATTCAATAATTTCAATTATCTCTCATTTGCGTTCTGGGGAACTGTAACCCTTAAATTCTGAAGATTGGAAAATACGGCCTAAGTAAATTGATGCTGTTTGGAGCTGGTTACGCCTTTATTTTCCTCATTTAGCGAGTACTGCGAAGTTTCACGGGTGCATAGGGAAAATTTATTGAATGTGGCTATAATCAAGGGTGCGTAATTATCGGTTACATTGAAGCAGTCTCCTGCAATAATTTCTTGGAAATAGGAAAGTTGAATGGGGAGATAATTAGTTGAGTTTTGCTTAATTATGGAAAAAGGAGGTTTAATTGAACTTGATATTGGTTTGTAGGTCCATCTGTTGTGTGAGAATTAATGCTCATTTAAACATAATCTTAAAGAAATTCATCAAAGTTCAAGTTCGTTAAAATATTTGTTGGCAGTGGCTGTGGGGAAGTTATTATGAAATTCAACGTCAGCTATTCTGGGACACTCATTTTATTCTAGTTTCTTGAAGTTTTAAAGTGAAACTCAGAGGTTCGATGCTTCTTTGTGGGCATTGGTCACTAGGAATTTTTGTAATTATGTCATTGATCTTGTTAGTTTGGGCTCGAGAACATTTTGTAGTTAGTGGAGGACACATACTTCTTTACGGCTTATTCTTTATATTGTTCTTGTATTCTTTATTTGTTTTACTCAAACAGAAGTGAAAGTTAGAGATGGAAGTGAATTTCTGAGTCTCATGTGTTTACCGATTGGATTAAGTGTAACTCTTACAACTTTTAATTTAATACTTGAAAGGATCTTCTTTTATTGTCGCGTTGATTGTTCCAACTATAAAAAAGAAGTCCATCTAAGTTATTAGAAGCCTCTTTTGGTGTAATCTAAGGTGTTATTGGTTTCTGTTTACAGTCAACACGACTCCCAGTGGGTTTATTTGCTGCCCAGTGTGAGGAATGCTTCAAATGGAGGTTAATAAGTACCCAAGAGGAGTATGAAGGTATTAGAAGCAAAATTATAGAAGAACCTTTCACTTGCCAAAGGAAAACCGATACTTCTTGTGAAGATCCTCCCGATATTGACTATGATACAACTCGAACATGGGGAATTGACAAACCCAACATCCCAAAGACCCCAGAAGGTTTCACAAGACGTTTAGTGCTGAGAAAGAACTTCACTAAATTCGATGCTTACTATGTTACACCCACAGGGAAGACAGTGAGATCTTCAACAGAGGTGTTAGCTTTTGTAGAAGCAAATCCTCAATACAAAGATATAGCTCTTTCAAACTTCAGTTTTGCTGTCCCAAAAATTATGGAAGAAACTGTTCCTGAAAGCATCTTGAAAGAGGGTTTAAATAGCAGCAGTGCCAAAAGAATTAAGAAAACAAAGAATGAATCTGCCCATACTGAATGAAAATCACTAGGGATTTGCTCGTAGCATTCTATTGTTGATCTTAACCCTTGTGAATGATTTGATATTGTGCCGAAAGATGTAGAATGATGATTCTCCACTATTGTTTGTACCATTTTCTTGTTCACTTATGAGGAGAGTTGGGGTATATGGTATCATATTGCCATGTTTGATGACTGTCTGAAGATTGTTGGACTGAGTGAATTTGGTGACAGCAATGAGCAGTGACAAATATCTAGGAAAAATAATCCATTTTATCCCATAAACATAGTAAATGGTAGGTAAGAATGGAGGATGATTAGGTGCGACA

mRNA sequence

CGGAAAGATTAATAAATTTTTCTTTAAAGCACAATTTACTTTTAAGATTTGTTATTAGAGATCACGCCATAGACGAGGCGAAATGGCCGCCGCCGCCGACAGACCTCCCAAAACGCCGACAAAGTCAACACGACTCCCAGTGGGTTTATTTGCTGCCCAGTGTGAGGAATGCTTCAAATGGAGGTTAATAAGTACCCAAGAGGAGTATGAAGGTATTAGAAGCAAAATTATAGAAGAACCTTTCACTTGCCAAAGGAAAACCGATACTTCTTGTGAAGATCCTCCCGATATTGACTATGATACAACTCGAACATGGGGAATTGACAAACCCAACATCCCAAAGACCCCAGAAGGTTTCACAAGACGTTTAGTGCTGAGAAAGAACTTCACTAAATTCGATGCTTACTATGTTACACCCACAGGGAAGACAGTGAGATCTTCAACAGAGGTGTTAGCTTTTGTAGAAGCAAATCCTCAATACAAAGATATAGCTCTTTCAAACTTCAGTTTTGCTGTCCCAAAAATTATGGAAGAAACTGTTCCTGAAAGCATCTTGAAAGAGGGTTTAAATAGCAGCAGTGCCAAAAGAATTAAGAAAACAAAGAATGAATCTGCCCATACTGAATGAAAATCACTAGGGATTTGCTCGTAGCATTCTATTGTTGATCTTAACCCTTGTGAATGATTTGATATTGTGCCGAAAGATGTAGAATGATGATTCTCCACTATTGTTTGTACCATTTTCTTGTTCACTTATGAGGAGAGTTGGGGTATATGGTATCATATTGCCATGTTTGATGACTGTCTGAAGATTGTTGGACTGAGTGAATTTGGTGACAGCAATGAGCAGTGACAAATATCTAGGAAAAATAATCCATTTTATCCCATAAACATAGTAAATGGTAGGTAAGAATGGAGGATGATTAGGTGCGACA

Coding sequence (CDS)

ATGGCCGCCGCCGCCGACAGACCTCCCAAAACGCCGACAAAGTCAACACGACTCCCAGTGGGTTTATTTGCTGCCCAGTGTGAGGAATGCTTCAAATGGAGGTTAATAAGTACCCAAGAGGAGTATGAAGGTATTAGAAGCAAAATTATAGAAGAACCTTTCACTTGCCAAAGGAAAACCGATACTTCTTGTGAAGATCCTCCCGATATTGACTATGATACAACTCGAACATGGGGAATTGACAAACCCAACATCCCAAAGACCCCAGAAGGTTTCACAAGACGTTTAGTGCTGAGAAAGAACTTCACTAAATTCGATGCTTACTATGTTACACCCACAGGGAAGACAGTGAGATCTTCAACAGAGGTGTTAGCTTTTGTAGAAGCAAATCCTCAATACAAAGATATAGCTCTTTCAAACTTCAGTTTTGCTGTCCCAAAAATTATGGAAGAAACTGTTCCTGAAAGCATCTTGAAAGAGGGTTTAAATAGCAGCAGTGCCAAAAGAATTAAGAAAACAAAGAATGAATCTGCCCATACTGAATGA

Protein sequence

MAAAADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDTSCEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSSTEVLAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHTE*

Homology

[Close]

BLAST of CSPI07G16700.1 vs. ExPASy Swiss-Prot
Match: Q9LYB9 (Methyl-CpG-binding domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=MBD4 PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 3.9e-48
Identity = 86/151 (56.95%), Postives = 114/151 (75.50%), Query Frame = 0


Query: 7   RPPKTPTKSTRLP---VGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDTS 66
           +P K   K    P   +  +AAQC+ C KWR+I +QEEYE IRSK++E+PF CQ+K   S
Sbjct: 9   KPAKPKAKKDVAPGRLIDTYAAQCDNCHKWRVIDSQEEYEDIRSKMLEDPFNCQKKQGMS 68

Query: 67  CEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSSTEV 126
           CE+P DIDYD++RTW IDKP +PKTP+GF R LVLRK+++K D YY TPTGK +RS  E+
Sbjct: 69  CEEPADIDYDSSRTWVIDKPGLPKTPKGFKRSLVLRKDYSKMDTYYFTPTGKKLRSRNEI 128

Query: 127 LAFVEANPQYKDIALSNFSFAVPKIMEETVP 155
            AFVEANP++++  L +F+F VPK+ME+TVP
Sbjct: 129 AAFVEANPEFRNAPLGDFNFTVPKVMEDTVP 159

[Close]

BLAST of CSPI07G16700.1 vs. ExPASy Swiss-Prot
Match: Q5XEN5 (Methyl-CpG-binding domain-containing protein 1 OS=Arabidopsis thaliana OX=3702 GN=MBD1 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.4e-45
Identity = 84/158 (53.16%), Postives = 113/158 (71.52%), Query Frame = 0


Query: 11  TPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDTSCEDPPDI 70
           TP +S    + +FA QCE+C KWR I TQ+EYE IRS++ E+PF C+ K   SCED  D+
Sbjct: 47  TPGRS----IDVFAVQCEKCMKWRKIDTQDEYEDIRSRVQEDPFFCKTKEGVSCEDVGDL 106

Query: 71  DYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSSTEVLAFVEAN 130
           +YD++RTW IDKP +P+TP GF R L+LRK+++K DAYY+TPTGK ++S  E+ AF++AN
Sbjct: 107 NYDSSRTWVIDKPGLPRTPRGFKRSLILRKDYSKMDAYYITPTGKKLKSRNEIAAFIDAN 166

Query: 131 PQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAK 169
             YK   L +F+F VPK+MEETVP  IL +     S K
Sbjct: 167 QDYKYALLGDFNFTVPKVMEETVPSGILSDRTPKPSRK 200

[Close]

BLAST of CSPI07G16700.1 vs. ExPASy Swiss-Prot
Match: Q8LA53 (Methyl-CpG-binding domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=MBD2 PE=1 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 5.0e-27
Identity = 62/140 (44.29%), Postives = 89/140 (63.57%), Query Frame = 0


Query: 20  VGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTC----QRKTDTSCEDPPDIDYDTT 79
           +G F  QC  CFKWRL+ + ++YE IR +++E PF C    + K D SC+ P DI  D T
Sbjct: 56  IGAFTVQCASCFKWRLMPSMQKYEEIREQLLENPFFCDTAREWKPDISCDVPADIYQDGT 115

Query: 80  RTWGIDKPNIPKTPEGFTRRLVLR-KNFTKF-DAYYVTPTGKTVRSSTEVLAFVEANPQY 139
           R W IDKPNI + P G+ R L +R +  T+F D YYV P+GK +RS+ EV  ++  N +Y
Sbjct: 116 RLWAIDKPNISRPPAGWQRLLRIRGEGGTRFADVYYVAPSGKKLRSTVEVQKYLNDNSEY 175

Query: 140 --KDIALSNFSFAVPKIMEE 152
             + + LS FSF +PK +++
Sbjct: 176 IGEGVKLSQFSFQIPKPLQD 195

[Close]

BLAST of CSPI07G16700.1 vs. ExPASy Swiss-Prot
Match: Q4PSK1 (Methyl-CpG-binding domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=MBD3 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.4e-21
Identity = 62/152 (40.79%), Postives = 91/152 (59.87%), Query Frame = 0


Query: 23  FAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDTSCEDPPD--IDYDT-TRTWG 82
           +AAQC +C K R I +QE+YE IRSK +E+ F C+R     CE+P D  +++D+ T  W 
Sbjct: 12  YAAQCWKCLKVRSIESQEDYEEIRSKTLEKFFECKR-----CEEPGDMVMNFDSLTMRWF 71

Query: 83  IDKPNIPKTPEGFTRRLVLRKNFTKFDAYY--VTPTGKTVRSSTEVLAFVEANPQYKDIA 142
            D+ +IPKTP+G  R LV+R N  K D YY  + P  K  +S  EV  F+E   ++KD+ 
Sbjct: 72  QDEHSIPKTPQGLKRVLVVRTNCVKVDVYYESLAPRRKRFKSIKEVATFIEDKEEFKDMT 131

Query: 143 LSNFSFAVPKIMEETVPESILKEGLNSSSAKR 170
           L   SFA PK ++      + K+ ++S S+ R
Sbjct: 132 LEEVSFAAPKRLK------LKKKPVDSHSSSR 152

[Close]

BLAST of CSPI07G16700.1 vs. ExPASy Swiss-Prot
Match: Q9FZP6 (Putative methyl-CpG-binding domain protein 12 OS=Arabidopsis thaliana OX=3702 GN=MBD12 PE=3 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 5.2e-16
Identity = 49/133 (36.84%), Postives = 76/133 (57.14%), Query Frame = 0


Query: 26  QCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKT----DTSCEDPPDIDYDTTRTWGID 85
           QC +C KWRLI + + Y  I+   ++ PF C   +    + SC  P   D  T  TW   
Sbjct: 3   QCTDCKKWRLIPSMQHYNIIKETQLQTPFVCGTTSGWTPNMSCNVPQ--DGTTCDTW--- 62

Query: 86  KPNIPKTPEGFTRRLVLRKNFTKF-DAYYVTPTGKTVRSSTEVLAFVEANPQY--KDIAL 145
            P+IP  P G++R + +R   TKF D YY  P+G+ +RSS EV +F++ +P+Y  + +  
Sbjct: 63  -PSIPPIPTGWSRSVHIRSESTKFADVYYFPPSGERLRSSAEVQSFLDNHPEYVREGVNR 122

Query: 146 SNFSFAVPKIMEE 152
           S FSF +PK +++
Sbjct: 123 SQFSFQIPKPLDD 129

[Close]

BLAST of CSPI07G16700.1 vs. ExPASy TrEMBL
Match: A0A0A0K900 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G391290 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 5.6e-98
Identity = 181/181 (100.00%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 1   MAAAADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKT 60
           MAAAADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKT
Sbjct: 1   MAAAADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKT 60

Query: 61  DTSCEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSS 120
           DTSCEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSS
Sbjct: 61  DTSCEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSS 120

Query: 121 TEVLAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHT 180
           TEVLAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHT
Sbjct: 121 TEVLAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHT 180

Query: 181 E 182
           E
Sbjct: 181 E 181

[Close]

BLAST of CSPI07G16700.1 vs. ExPASy TrEMBL
Match: A0A5A7UVI7 (Methyl-CpG-binding domain-containing protein 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G002230 PE=4 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 1.8e-91
Identity = 168/179 (93.85%), Postives = 175/179 (97.77%), Query Frame = 0


Query: 3   AAADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDT 62
           AAA RPPKTP KSTR+PVGLFAAQCEECFKWRLISTQEEYE IRS++IEEPFTCQR+TDT
Sbjct: 2   AAAARPPKTPIKSTRVPVGLFAAQCEECFKWRLISTQEEYEDIRSRMIEEPFTCQRRTDT 61

Query: 63  SCEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSSTE 122
           SCEDPPDI+YDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRS TE
Sbjct: 62  SCEDPPDINYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSPTE 121

Query: 123 VLAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHTE 182
           +LAFV+ANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHTE
Sbjct: 122 ILAFVQANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHTE 180

[Close]

BLAST of CSPI07G16700.1 vs. ExPASy TrEMBL
Match: A0A1S3BRE2 (methyl-CpG-binding domain-containing protein 4 OS=Cucumis melo OX=3656 GN=LOC103492710 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 5.6e-90
Identity = 166/179 (92.74%), Postives = 173/179 (96.65%), Query Frame = 0


Query: 3   AAADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDT 62
           AAA RPPKTP KSTR+PV LFA QCEECFKWRLISTQEEYE IRS++IEEPFTCQRKTDT
Sbjct: 2   AAAARPPKTPIKSTRVPVSLFAVQCEECFKWRLISTQEEYEDIRSRMIEEPFTCQRKTDT 61

Query: 63  SCEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSSTE 122
           SCEDPPDI+YDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRS TE
Sbjct: 62  SCEDPPDINYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSPTE 121

Query: 123 VLAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHTE 182
           +LAFV+ANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESA+TE
Sbjct: 122 ILAFVQANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAYTE 180

[Close]

BLAST of CSPI07G16700.1 vs. ExPASy TrEMBL
Match: A0A6J1GNJ9 (methyl-CpG-binding domain-containing protein 4-like OS=Cucurbita moschata OX=3662 GN=LOC111456046 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 6.9e-80
Identity = 145/178 (81.46%), Postives = 165/178 (92.70%), Query Frame = 0


Query: 4   AADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDTS 63
           AA RP +TP KSTRLPVGL+AAQCEECFKWRLISTQEEYE IRSK+IEEPFTC RKTDTS
Sbjct: 2   AAARPTRTPMKSTRLPVGLYAAQCEECFKWRLISTQEEYEDIRSKLIEEPFTCHRKTDTS 61

Query: 64  CEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSSTEV 123
           C+DPPDI+YD TRTWGIDKPNIPKTPEGFTR LV+RKNF+KFDAYYVTPTGKTVRS TE+
Sbjct: 62  CKDPPDIEYDATRTWGIDKPNIPKTPEGFTRHLVVRKNFSKFDAYYVTPTGKTVRSPTEM 121

Query: 124 LAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHTE 182
            AF+ ANP++KD+ L++FSF +P++MEETVPESILKEGL+SSSAKRIKKTK+E AHT+
Sbjct: 122 QAFLGANPRHKDVPLTDFSFTIPRLMEETVPESILKEGLDSSSAKRIKKTKDEFAHTD 179

[Close]

BLAST of CSPI07G16700.1 vs. ExPASy TrEMBL
Match: A0A6J1D952 (methyl-CpG-binding domain-containing protein 4-like OS=Momordica charantia OX=3673 GN=LOC111018404 PE=4 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.9e-78
Identity = 145/178 (81.46%), Postives = 161/178 (90.45%), Query Frame = 0


Query: 4   AADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDTS 63
           A  RPP+ P KS RLPV L+AAQCEECFKWRLISTQEEYE IRSK+IEEPFTC RKTDTS
Sbjct: 27  AGARPPRKPVKSKRLPVDLYAAQCEECFKWRLISTQEEYEDIRSKLIEEPFTCNRKTDTS 86

Query: 64  CEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSSTEV 123
           CED  DIDYD TRTW IDKPNIPKTPEGF RRLVLRK+F+KFDAYY+TPTGKTVRSSTE+
Sbjct: 87  CEDASDIDYDATRTWVIDKPNIPKTPEGFKRRLVLRKDFSKFDAYYITPTGKTVRSSTEM 146

Query: 124 LAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHTE 182
           L+F+EANP+YKDI LS FSFAVPK+MEETVPESIL+E L+SSSAKRIKK K++SAHT+
Sbjct: 147 LSFIEANPKYKDIPLSKFSFAVPKVMEETVPESILREDLSSSSAKRIKKVKDKSAHTD 204

[Close]

BLAST of CSPI07G16700.1 vs. NCBI nr
Match: XP_004148873.1 (methyl-CpG-binding domain-containing protein 4 [Cucumis sativus] >KGN44852.1 hypothetical protein Csa_016159 [Cucumis sativus])

HSP 1 Score: 366.7 bits (940), Expect = 1.2e-97
Identity = 181/181 (100.00%), Postives = 181/181 (100.00%), Query Frame = 0


Query: 1   MAAAADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKT 60
           MAAAADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKT
Sbjct: 1   MAAAADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKT 60

Query: 61  DTSCEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSS 120
           DTSCEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSS
Sbjct: 61  DTSCEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSS 120

Query: 121 TEVLAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHT 180
           TEVLAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHT
Sbjct: 121 TEVLAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHT 180

Query: 181 E 182
           E
Sbjct: 181 E 181

[Close]

BLAST of CSPI07G16700.1 vs. NCBI nr
Match: KAA0057559.1 (methyl-CpG-binding domain-containing protein 4 [Cucumis melo var. makuwa] >TYK18280.1 methyl-CpG-binding domain-containing protein 4 [Cucumis melo var. makuwa])

HSP 1 Score: 345.1 bits (884), Expect = 3.6e-91
Identity = 168/179 (93.85%), Postives = 175/179 (97.77%), Query Frame = 0


Query: 3   AAADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDT 62
           AAA RPPKTP KSTR+PVGLFAAQCEECFKWRLISTQEEYE IRS++IEEPFTCQR+TDT
Sbjct: 2   AAAARPPKTPIKSTRVPVGLFAAQCEECFKWRLISTQEEYEDIRSRMIEEPFTCQRRTDT 61

Query: 63  SCEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSSTE 122
           SCEDPPDI+YDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRS TE
Sbjct: 62  SCEDPPDINYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSPTE 121

Query: 123 VLAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHTE 182
           +LAFV+ANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHTE
Sbjct: 122 ILAFVQANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHTE 180

[Close]

BLAST of CSPI07G16700.1 vs. NCBI nr
Match: XP_008451407.1 (PREDICTED: methyl-CpG-binding domain-containing protein 4 [Cucumis melo])

HSP 1 Score: 340.1 bits (871), Expect = 1.2e-89
Identity = 166/179 (92.74%), Postives = 173/179 (96.65%), Query Frame = 0


Query: 3   AAADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDT 62
           AAA RPPKTP KSTR+PV LFA QCEECFKWRLISTQEEYE IRS++IEEPFTCQRKTDT
Sbjct: 2   AAAARPPKTPIKSTRVPVSLFAVQCEECFKWRLISTQEEYEDIRSRMIEEPFTCQRKTDT 61

Query: 63  SCEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSSTE 122
           SCEDPPDI+YDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRS TE
Sbjct: 62  SCEDPPDINYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSPTE 121

Query: 123 VLAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHTE 182
           +LAFV+ANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESA+TE
Sbjct: 122 ILAFVQANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAYTE 180

[Close]

BLAST of CSPI07G16700.1 vs. NCBI nr
Match: XP_038896997.1 (methyl-CpG-binding domain-containing protein 4-like [Benincasa hispida])

HSP 1 Score: 331.6 bits (849), Expect = 4.1e-87
Identity = 161/177 (90.96%), Postives = 170/177 (96.05%), Query Frame = 0


Query: 4   AADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDTS 63
           AA RPPKTP KS RLPVGLFAAQCEECFKWRLISTQEEYE IRSK+IEEPFTC RKTDTS
Sbjct: 31  AAARPPKTPVKSARLPVGLFAAQCEECFKWRLISTQEEYEDIRSKMIEEPFTCPRKTDTS 90

Query: 64  CEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSSTEV 123
           C++PPDIDYDTTRTWGIDKPNIPKTP GFTRRLVLRKNF+KFDAYYVTPTGKTVRSSTE+
Sbjct: 91  CDNPPDIDYDTTRTWGIDKPNIPKTPHGFTRRLVLRKNFSKFDAYYVTPTGKTVRSSTEM 150

Query: 124 LAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHT 181
           LAFVEANP+YKDI LS+FSFAVPK+MEETVPESILKEGLNSSSAKRIKKTK+ESAHT
Sbjct: 151 LAFVEANPEYKDIPLSSFSFAVPKVMEETVPESILKEGLNSSSAKRIKKTKDESAHT 207

[Close]

BLAST of CSPI07G16700.1 vs. NCBI nr
Match: XP_022953528.1 (methyl-CpG-binding domain-containing protein 4-like [Cucurbita moschata])

HSP 1 Score: 306.6 bits (784), Expect = 1.4e-79
Identity = 145/178 (81.46%), Postives = 165/178 (92.70%), Query Frame = 0


Query: 4   AADRPPKTPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDTS 63
           AA RP +TP KSTRLPVGL+AAQCEECFKWRLISTQEEYE IRSK+IEEPFTC RKTDTS
Sbjct: 2   AAARPTRTPMKSTRLPVGLYAAQCEECFKWRLISTQEEYEDIRSKLIEEPFTCHRKTDTS 61

Query: 64  CEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSSTEV 123
           C+DPPDI+YD TRTWGIDKPNIPKTPEGFTR LV+RKNF+KFDAYYVTPTGKTVRS TE+
Sbjct: 62  CKDPPDIEYDATRTWGIDKPNIPKTPEGFTRHLVVRKNFSKFDAYYVTPTGKTVRSPTEM 121

Query: 124 LAFVEANPQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAKRIKKTKNESAHTE 182
            AF+ ANP++KD+ L++FSF +P++MEETVPESILKEGL+SSSAKRIKKTK+E AHT+
Sbjct: 122 QAFLGANPRHKDVPLTDFSFTIPRLMEETVPESILKEGLDSSSAKRIKKTKDEFAHTD 179

[Close]

BLAST of CSPI07G16700.1 vs. TAIR 10
Match: AT3G63030.1 (methyl-CPG-binding domain 4 )

HSP 1 Score: 192.6 bits (488), Expect = 2.8e-49
Identity = 86/151 (56.95%), Postives = 114/151 (75.50%), Query Frame = 0


Query: 7   RPPKTPTKSTRLP---VGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDTS 66
           +P K   K    P   +  +AAQC+ C KWR+I +QEEYE IRSK++E+PF CQ+K   S
Sbjct: 9   KPAKPKAKKDVAPGRLIDTYAAQCDNCHKWRVIDSQEEYEDIRSKMLEDPFNCQKKQGMS 68

Query: 67  CEDPPDIDYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSSTEV 126
           CE+P DIDYD++RTW IDKP +PKTP+GF R LVLRK+++K D YY TPTGK +RS  E+
Sbjct: 69  CEEPADIDYDSSRTWVIDKPGLPKTPKGFKRSLVLRKDYSKMDTYYFTPTGKKLRSRNEI 128

Query: 127 LAFVEANPQYKDIALSNFSFAVPKIMEETVP 155
            AFVEANP++++  L +F+F VPK+ME+TVP
Sbjct: 129 AAFVEANPEFRNAPLGDFNFTVPKVMEDTVP 159

[Close]

BLAST of CSPI07G16700.1 vs. TAIR 10
Match: AT4G22745.1 (methyl-CPG-binding domain 1 )

HSP 1 Score: 184.1 bits (466), Expect = 1.0e-46
Identity = 84/158 (53.16%), Postives = 113/158 (71.52%), Query Frame = 0


Query: 11  TPTKSTRLPVGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTCQRKTDTSCEDPPDI 70
           TP +S    + +FA QCE+C KWR I TQ+EYE IRS++ E+PF C+ K   SCED  D+
Sbjct: 47  TPGRS----IDVFAVQCEKCMKWRKIDTQDEYEDIRSRVQEDPFFCKTKEGVSCEDVGDL 106

Query: 71  DYDTTRTWGIDKPNIPKTPEGFTRRLVLRKNFTKFDAYYVTPTGKTVRSSTEVLAFVEAN 130
           +YD++RTW IDKP +P+TP GF R L+LRK+++K DAYY+TPTGK ++S  E+ AF++AN
Sbjct: 107 NYDSSRTWVIDKPGLPRTPRGFKRSLILRKDYSKMDAYYITPTGKKLKSRNEIAAFIDAN 166

Query: 131 PQYKDIALSNFSFAVPKIMEETVPESILKEGLNSSSAK 169
             YK   L +F+F VPK+MEETVP  IL +     S K
Sbjct: 167 QDYKYALLGDFNFTVPKVMEETVPSGILSDRTPKPSRK 200

[Close]

BLAST of CSPI07G16700.1 vs. TAIR 10
Match: AT5G35330.1 (methyl-CPG-binding domain protein 02 )

HSP 1 Score: 122.5 bits (306), Expect = 3.6e-28
Identity = 62/140 (44.29%), Postives = 89/140 (63.57%), Query Frame = 0


Query: 20  VGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTC----QRKTDTSCEDPPDIDYDTT 79
           +G F  QC  CFKWRL+ + ++YE IR +++E PF C    + K D SC+ P DI  D T
Sbjct: 56  IGAFTVQCASCFKWRLMPSMQKYEEIREQLLENPFFCDTAREWKPDISCDVPADIYQDGT 115

Query: 80  RTWGIDKPNIPKTPEGFTRRLVLR-KNFTKF-DAYYVTPTGKTVRSSTEVLAFVEANPQY 139
           R W IDKPNI + P G+ R L +R +  T+F D YYV P+GK +RS+ EV  ++  N +Y
Sbjct: 116 RLWAIDKPNISRPPAGWQRLLRIRGEGGTRFADVYYVAPSGKKLRSTVEVQKYLNDNSEY 175

Query: 140 --KDIALSNFSFAVPKIMEE 152
             + + LS FSF +PK +++
Sbjct: 176 IGEGVKLSQFSFQIPKPLQD 195

[Close]

BLAST of CSPI07G16700.1 vs. TAIR 10
Match: AT5G35330.2 (methyl-CPG-binding domain protein 02 )

HSP 1 Score: 122.5 bits (306), Expect = 3.6e-28
Identity = 62/140 (44.29%), Postives = 89/140 (63.57%), Query Frame = 0


Query: 20  VGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTC----QRKTDTSCEDPPDIDYDTT 79
           +G F  QC  CFKWRL+ + ++YE IR +++E PF C    + K D SC+ P DI  D T
Sbjct: 56  IGAFTVQCASCFKWRLMPSMQKYEEIREQLLENPFFCDTAREWKPDISCDVPADIYQDGT 115

Query: 80  RTWGIDKPNIPKTPEGFTRRLVLR-KNFTKF-DAYYVTPTGKTVRSSTEVLAFVEANPQY 139
           R W IDKPNI + P G+ R L +R +  T+F D YYV P+GK +RS+ EV  ++  N +Y
Sbjct: 116 RLWAIDKPNISRPPAGWQRLLRIRGEGGTRFADVYYVAPSGKKLRSTVEVQKYLNDNSEY 175

Query: 140 --KDIALSNFSFAVPKIMEE 152
             + + LS FSF +PK +++
Sbjct: 176 IGEGVKLSQFSFQIPKPLQD 195

[Close]

BLAST of CSPI07G16700.1 vs. TAIR 10
Match: AT5G35330.3 (methyl-CPG-binding domain protein 02 )

HSP 1 Score: 122.5 bits (306), Expect = 3.6e-28
Identity = 62/140 (44.29%), Postives = 89/140 (63.57%), Query Frame = 0


Query: 20  VGLFAAQCEECFKWRLISTQEEYEGIRSKIIEEPFTC----QRKTDTSCEDPPDIDYDTT 79
           +G F  QC  CFKWRL+ + ++YE IR +++E PF C    + K D SC+ P DI  D T
Sbjct: 56  IGAFTVQCASCFKWRLMPSMQKYEEIREQLLENPFFCDTAREWKPDISCDVPADIYQDGT 115

Query: 80  RTWGIDKPNIPKTPEGFTRRLVLR-KNFTKF-DAYYVTPTGKTVRSSTEVLAFVEANPQY 139
           R W IDKPNI + P G+ R L +R +  T+F D YYV P+GK +RS+ EV  ++  N +Y
Sbjct: 116 RLWAIDKPNISRPPAGWQRLLRIRGEGGTRFADVYYVAPSGKKLRSTVEVQKYLNDNSEY 175

Query: 140 --KDIALSNFSFAVPKIMEE 152
             + + LS FSF +PK +++
Sbjct: 176 IGEGVKLSQFSFQIPKPLQD 195

The following BLAST results are available for this feature:

BLAST of CSPI07G16700.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LYB9	3.9e-48	56.95	Methyl-CpG-binding domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q5XEN5	1.4e-45	53.16	Methyl-CpG-binding domain-containing protein 1 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q8LA53	5.0e-27	44.29	Methyl-CpG-binding domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q4PSK1	2.4e-21	40.79	Methyl-CpG-binding domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q9FZP6	5.2e-16	36.84	Putative methyl-CpG-binding domain protein 12 OS=Arabidopsis thaliana OX=3702 GN...	[more]

BLAST of CSPI07G16700.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K900	5.6e-98	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G391290 PE=4 SV=1	[more]
A0A5A7UVI7	1.8e-91	93.85	Methyl-CpG-binding domain-containing protein 4 OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3BRE2	5.6e-90	92.74	methyl-CpG-binding domain-containing protein 4 OS=Cucumis melo OX=3656 GN=LOC103...	[more]
A0A6J1GNJ9	6.9e-80	81.46	methyl-CpG-binding domain-containing protein 4-like OS=Cucurbita moschata OX=366...	[more]
A0A6J1D952	2.9e-78	81.46	methyl-CpG-binding domain-containing protein 4-like OS=Momordica charantia OX=36...	[more]

BLAST of CSPI07G16700.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148873.1	1.2e-97	100.00	methyl-CpG-binding domain-containing protein 4 [Cucumis sativus] >KGN44852.1 hyp...	[more]
KAA0057559.1	3.6e-91	93.85	methyl-CpG-binding domain-containing protein 4 [Cucumis melo var. makuwa] >TYK18...	[more]
XP_008451407.1	1.2e-89	92.74	PREDICTED: methyl-CpG-binding domain-containing protein 4 [Cucumis melo]	[more]
XP_038896997.1	4.1e-87	90.96	methyl-CpG-binding domain-containing protein 4-like [Benincasa hispida]	[more]
XP_022953528.1	1.4e-79	81.46	methyl-CpG-binding domain-containing protein 4-like [Cucurbita moschata]	[more]

BLAST of CSPI07G16700.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G63030.1	2.8e-49	56.95	methyl-CPG-binding domain 4	[more]
AT4G22745.1	1.0e-46	53.16	methyl-CPG-binding domain 1	[more]
AT5G35330.1	3.6e-28	44.29	methyl-CPG-binding domain protein 02	[more]
AT5G35330.2	3.6e-28	44.29	methyl-CPG-binding domain protein 02	[more]
AT5G35330.3	3.6e-28	44.29	methyl-CPG-binding domain protein 02	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001739	Methyl-CpG DNA binding	SMART	SM00391	TAM_2	coord: 80..153 e-value: 6.6E-9 score: 45.6
IPR001739	Methyl-CpG DNA binding	PFAM	PF01429	MBD	coord: 87..149 e-value: 1.7E-12 score: 46.9
IPR001739	Methyl-CpG DNA binding	PROSITE	PS50982	MBD	coord: 78..148 score: 13.887215
IPR011124	Zinc finger, CW-type	PFAM	PF07496	zf-CW	coord: 24..69 e-value: 1.9E-10 score: 40.6
IPR011124	Zinc finger, CW-type	PROSITE	PS51050	ZF_CW	coord: 17..72 score: 11.637567
None	No IPR available	GENE3D	3.30.890.10		coord: 80..154 e-value: 4.1E-14 score: 54.1
None	No IPR available	GENE3D	3.30.40.100		coord: 23..71 e-value: 7.3E-6 score: 27.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 159..181
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 165..181
None	No IPR available	PANTHER	PTHR12396:SF10	METHYL-CPG-BINDING DOMAIN-CONTAINING PROTEIN 1-RELATED	coord: 12..175
None	No IPR available	PANTHER	PTHR12396	METHYL-CPG BINDING PROTEIN, MBD	coord: 12..175
None	No IPR available	CDD	cd01396	MeCP2_MBD	coord: 82..154 e-value: 1.28405E-20 score: 79.3389
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 60..153

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI07G16700	CSPI07G16700	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI07G16700.1.utr5p1	CSPI07G16700.1.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI07G16700.1.cds1	CSPI07G16700.1.cds1	CDS
CSPI07G16700.1.cds2	CSPI07G16700.1.cds2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI07G16700.1.utr3p1	CSPI07G16700.1.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI07G16700.1	CSPI07G16700.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0008270	zinc ion binding