CSPI07G15720.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI07G15720.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	PfkB domain-containing protein
Location	Chr7: 14357318 .. 14358059 (+)
Sequence length	742
RNA-Seq Expression	CSPI07G15720.1
Synteny	CSPI07G15720.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAAATATATGATACTGCTCCGTTGGATTGGATTGAGGGCAAAAGGAATGTTGGCTCTTGGCTCTTGGCTCTCGGCTCTCGCCATTGCCAATAAGCAGAACTCTTTTGAACAATAAACAATCCAACAGCAACTTTTGTAAGAGAGTCCCTTTCACTCTGCATTACCCATTTGGAAAATGAAGCTTTGAGTGGCCATAGGAATAAATTCCCAAAGAACCCATTTCTTGAATCATTTTTTTTACTGGTTTTTTCATATGCAGAGCTTGAAATTCCCACCGTTTCTTCTTCTTTCTCATCTTATTGCTTAATAAATAACCACATACCCATTTCGCTAATTTTCTTCTCTAATCGTCTTCAACCTTTGTCCGAATGCCGCTATCCGCCATTTCTTTCCAATCTTCACTCCATTGTTTCACTGGACTTCCTTTTCCTAGACCCTTTCGCCCCAATTTGGGGCTTCATCCTTCAATTCCTTCAAAACCCACTCGGGGTTTTCTCTGTTGCAAGAAGCTTCAAGTATCGGAGCCTAAGCATTATCCCAACTCTTTCAATTTCTCGAATGAGTGCGTTGCGATTCCTAAATTCAGGAGTTCGAGTTCGAAGGATATTGATGTTGCTACTCTTGGAAATCTTTGCGTGGATATCGTGCTTAATGTTCCTAGTTTACCTCCAGAAAATGATGATGAACGGCGGGCTTATATGGAGCGGTTGTCCTCCTCGCCTCCTGAAAAGGTTTGTTAA

mRNA sequence

GAAAAATATATGATACTGCTCCGTTGGATTGGATTGAGGGCAAAAGGAATGTTGGCTCTTGGCTCTTGGCTCTCGGCTCTCGCCATTGCCAATAAGCAGAACTCTTTTGAACAATAAACAATCCAACAGCAACTTTTGTAAGAGAGTCCCTTTCACTCTGCATTACCCATTTGGAAAATGAAGCTTTGAGTGGCCATAGGAATAAATTCCCAAAGAACCCATTTCTTGAATCATTTTTTTTACTGGTTTTTTCATATGCAGAGCTTGAAATTCCCACCGTTTCTTCTTCTTTCTCATCTTATTGCTTAATAAATAACCACATACCCATTTCGCTAATTTTCTTCTCTAATCGTCTTCAACCTTTGTCCGAATGCCGCTATCCGCCATTTCTTTCCAATCTTCACTCCATTGTTTCACTGGACTTCCTTTTCCTAGACCCTTTCGCCCCAATTTGGGGCTTCATCCTTCAATTCCTTCAAAACCCACTCGGGGTTTTCTCTGTTGCAAGAAGCTTCAAGTATCGGAGCCTAAGCATTATCCCAACTCTTTCAATTTCTCGAATGAGTGCGTTGCGATTCCTAAATTCAGGAGTTCGAGTTCGAAGGATATTGATGTTGCTACTCTTGGAAATCTTTGCGTGGATATCGTGCTTAATGTTCCTAGTTTACCTCCAGAAAATGATGATGAACGGCGGGCTTATATGGAGCGGTTGTCCTCCTCGCCTCCTGAAAAGGTTTGTTAA

Coding sequence (CDS)

ATGCCGCTATCCGCCATTTCTTTCCAATCTTCACTCCATTGTTTCACTGGACTTCCTTTTCCTAGACCCTTTCGCCCCAATTTGGGGCTTCATCCTTCAATTCCTTCAAAACCCACTCGGGGTTTTCTCTGTTGCAAGAAGCTTCAAGTATCGGAGCCTAAGCATTATCCCAACTCTTTCAATTTCTCGAATGAGTGCGTTGCGATTCCTAAATTCAGGAGTTCGAGTTCGAAGGATATTGATGTTGCTACTCTTGGAAATCTTTGCGTGGATATCGTGCTTAATGTTCCTAGTTTACCTCCAGAAAATGATGATGAACGGCGGGCTTATATGGAGCGGTTGTCCTCCTCGCCTCCTGAAAAGGTTTGTTAA

Protein sequence

MPLSAISFQSSLHCFTGLPFPRPFRPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSFNFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPEKVC*

Homology

[Close]

BLAST of CSPI07G15720.1 vs. ExPASy TrEMBL
Match: A0A0A0KAB6 (PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378400 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 7.3e-65
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 0


Query: 1   MPLSAISFQSSLHCFTGLPFPRPFRPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF 60
           MPLSAISFQSSLHCFTGLPFPRPFRPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF
Sbjct: 1   MPLSAISFQSSLHCFTGLPFPRPFRPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF 60

Query: 61  NFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPE 120
           NFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPE
Sbjct: 61  NFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPE 120

Query: 121 K 122
           K
Sbjct: 121 K 121

[Close]

BLAST of CSPI07G15720.1 vs. ExPASy TrEMBL
Match: A0A1S3BRU8 (fructokinase-1 OS=Cucumis melo OX=3656 GN=LOC103492613 PE=4 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 5.8e-62
Identity = 115/121 (95.04%), Postives = 119/121 (98.35%), Query Frame = 0


Query: 1   MPLSAISFQSSLHCFTGLPFPRPFRPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF 60
           MPLSAISFQSSL CFTGLPFP PF+PNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF
Sbjct: 1   MPLSAISFQSSLRCFTGLPFPTPFQPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF 60

Query: 61  NFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPE 120
           NFSNECVA+PKFRS+SSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPP+
Sbjct: 61  NFSNECVAVPKFRSASSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPD 120

Query: 121 K 122
           K
Sbjct: 121 K 121

[Close]

BLAST of CSPI07G15720.1 vs. ExPASy TrEMBL
Match: A0A6J1H739 (fructokinase-1 OS=Cucurbita moschata OX=3662 GN=LOC111461046 PE=4 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.6e-43
Identity = 86/121 (71.07%), Postives = 104/121 (85.95%), Query Frame = 0


Query: 1   MPLSAISFQSSLHCFTGLPFPRPFRPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF 60
           MPL+A+S  SS+HCFTGLP    F  + G+ PSI  KP+R FLCCKK++VSEPKH+ +SF
Sbjct: 1   MPLTAVSLDSSVHCFTGLPSRPGFYRSFGVRPSISPKPSRFFLCCKKVEVSEPKHFASSF 60

Query: 61  NFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPE 120
           NFSNE  A+ KFRS+++KDIDVATLGNLCVDIVLNVP+LPPE+DDER+AYMERLSSSPP+
Sbjct: 61  NFSNEGAAVSKFRSANAKDIDVATLGNLCVDIVLNVPNLPPEDDDERKAYMERLSSSPPD 120

Query: 121 K 122
           K
Sbjct: 121 K 121

[Close]

BLAST of CSPI07G15720.1 vs. ExPASy TrEMBL
Match: A0A6J1KXH7 (uncharacterized protein LOC111498032 OS=Cucurbita maxima OX=3661 GN=LOC111498032 PE=4 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 7.9e-43
Identity = 85/121 (70.25%), Postives = 103/121 (85.12%), Query Frame = 0


Query: 1   MPLSAISFQSSLHCFTGLPFPRPFRPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF 60
           MPL+A+S  SS+HCFTGLP       + G+ PSI  KP+R FLCCKK++VSEPKH+ +SF
Sbjct: 1   MPLTAVSLDSSVHCFTGLPSRPGLYRSFGVRPSISPKPSRFFLCCKKVEVSEPKHFASSF 60

Query: 61  NFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPE 120
           NFSNE  A+ KFRS+++KDIDVATLGNLCVDIVLNVP+LPPE+DDER+AYMERLSSSPP+
Sbjct: 61  NFSNEGAAVSKFRSANAKDIDVATLGNLCVDIVLNVPNLPPEDDDERKAYMERLSSSPPD 120

Query: 121 K 122
           K
Sbjct: 121 K 121

[Close]

BLAST of CSPI07G15720.1 vs. ExPASy TrEMBL
Match: A0A6J1DRF9 (fructokinase-1 OS=Momordica charantia OX=3673 GN=LOC111022867 PE=4 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 3.4e-38
Identity = 80/130 (61.54%), Postives = 100/130 (76.92%), Query Frame = 0


Query: 1   MPLSAISFQSSLHCFTGLPFPRPFRPNLGLH----PSIPSKPTRGFLCCKKLQVSEPKHY 60
           MP SA+S + ++ CFTGLP    F  + GLH    P +P  PT+ FLCCKKL++SEPKH+
Sbjct: 1   MPRSAVSLEPTVPCFTGLPSRPGFHRSFGLHQPIFPPLPRSPTQFFLCCKKLEISEPKHF 60

Query: 61  -----PNSFNFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYM 120
                 +S  + NECV + KFRS+++KDIDVATLGNLCVDIVLNVPSLPPENDDER+AYM
Sbjct: 61  ASSSSSSSLRYGNECVPVSKFRSANAKDIDVATLGNLCVDIVLNVPSLPPENDDERKAYM 120

Query: 121 ERLSSSPPEK 122
           E+LS+SPP+K
Sbjct: 121 EQLSASPPDK 130

[Close]

BLAST of CSPI07G15720.1 vs. NCBI nr
Match: XP_004146987.1 (fructokinase-1 [Cucumis sativus] >KAE8646296.1 hypothetical protein Csa_015704 [Cucumis sativus])

HSP 1 Score: 256.1 bits (653), Expect = 1.5e-64
Identity = 121/121 (100.00%), Postives = 121/121 (100.00%), Query Frame = 0


Query: 1   MPLSAISFQSSLHCFTGLPFPRPFRPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF 60
           MPLSAISFQSSLHCFTGLPFPRPFRPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF
Sbjct: 1   MPLSAISFQSSLHCFTGLPFPRPFRPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF 60

Query: 61  NFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPE 120
           NFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPE
Sbjct: 61  NFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPE 120

Query: 121 K 122
           K
Sbjct: 121 K 121

[Close]

BLAST of CSPI07G15720.1 vs. NCBI nr
Match: XP_008451263.1 (PREDICTED: fructokinase-1 [Cucumis melo])

HSP 1 Score: 246.5 bits (628), Expect = 1.2e-61
Identity = 115/121 (95.04%), Postives = 119/121 (98.35%), Query Frame = 0


Query: 1   MPLSAISFQSSLHCFTGLPFPRPFRPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF 60
           MPLSAISFQSSL CFTGLPFP PF+PNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF
Sbjct: 1   MPLSAISFQSSLRCFTGLPFPTPFQPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF 60

Query: 61  NFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPE 120
           NFSNECVA+PKFRS+SSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPP+
Sbjct: 61  NFSNECVAVPKFRSASSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPD 120

Query: 121 K 122
           K
Sbjct: 121 K 121

[Close]

BLAST of CSPI07G15720.1 vs. NCBI nr
Match: XP_038898169.1 (fructokinase-1 [Benincasa hispida])

HSP 1 Score: 229.2 bits (583), Expect = 2.0e-56
Identity = 107/121 (88.43%), Postives = 114/121 (94.21%), Query Frame = 0


Query: 1   MPLSAISFQSSLHCFTGLPFPRPFRPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF 60
           MPL+AI+FQSSLHCFTG PFP PF PNLG H SIPSKP+R FLCCKKLQVSEPKHYP+SF
Sbjct: 1   MPLTAIAFQSSLHCFTGFPFPPPFHPNLGFHSSIPSKPSRVFLCCKKLQVSEPKHYPSSF 60

Query: 61  NFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPE 120
           NFSNE VA+PKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDER+AYMERLSSSPP+
Sbjct: 61  NFSNESVAVPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERQAYMERLSSSPPD 120

Query: 121 K 122
           K
Sbjct: 121 K 121

[Close]

BLAST of CSPI07G15720.1 vs. NCBI nr
Match: XP_023514771.1 (uncharacterized protein LOC111778983 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 185.3 bits (469), Expect = 3.3e-43
Identity = 86/121 (71.07%), Postives = 104/121 (85.95%), Query Frame = 0


Query: 1   MPLSAISFQSSLHCFTGLPFPRPFRPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF 60
           MPL+A+S  SS+HCFTGLP    F  + G+ PSI  KP+R FLCCKK++VSEPKH+ +SF
Sbjct: 1   MPLTAVSLDSSVHCFTGLPSRPGFYRSFGVRPSISPKPSRFFLCCKKVEVSEPKHFASSF 60

Query: 61  NFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPE 120
           NFSNE  A+ KFRS+++KDIDVATLGNLCVDIVLNVP+LPPE+DDER+AYMERLSSSPP+
Sbjct: 61  NFSNEGAAVSKFRSANAKDIDVATLGNLCVDIVLNVPNLPPEDDDERKAYMERLSSSPPD 120

Query: 121 K 122
           K
Sbjct: 121 K 121

[Close]

BLAST of CSPI07G15720.1 vs. NCBI nr
Match: XP_022960246.1 (fructokinase-1 [Cucurbita moschata] >XP_022960247.1 fructokinase-1 [Cucurbita moschata])

HSP 1 Score: 185.3 bits (469), Expect = 3.3e-43
Identity = 86/121 (71.07%), Postives = 104/121 (85.95%), Query Frame = 0


Query: 1   MPLSAISFQSSLHCFTGLPFPRPFRPNLGLHPSIPSKPTRGFLCCKKLQVSEPKHYPNSF 60
           MPL+A+S  SS+HCFTGLP    F  + G+ PSI  KP+R FLCCKK++VSEPKH+ +SF
Sbjct: 1   MPLTAVSLDSSVHCFTGLPSRPGFYRSFGVRPSISPKPSRFFLCCKKVEVSEPKHFASSF 60

Query: 61  NFSNECVAIPKFRSSSSKDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPE 120
           NFSNE  A+ KFRS+++KDIDVATLGNLCVDIVLNVP+LPPE+DDER+AYMERLSSSPP+
Sbjct: 61  NFSNEGAAVSKFRSANAKDIDVATLGNLCVDIVLNVPNLPPEDDDERKAYMERLSSSPPD 120

Query: 121 K 122
           K
Sbjct: 121 K 121

[Close]

BLAST of CSPI07G15720.1 vs. TAIR 10
Match: AT1G06730.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 61.2 bits (147), Expect = 6.6e-10
Identity = 29/44 (65.91%), Postives = 35/44 (79.55%), Query Frame = 0


Query: 78  KDIDVATLGNLCVDIVLNVPSLPPENDDERRAYMERLSSSPPEK 122
           K IDV+TLGNLCVDIVL+V  LPP +  ER+A M+ LS SPP+K
Sbjct: 83  KPIDVSTLGNLCVDIVLSVHELPPPSRGERKALMDELSMSPPDK 126

The following BLAST results are available for this feature:

BLAST of CSPI07G15720.1 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI07G15720.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KAB6	7.3e-65	100.00	PfkB domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G378400 PE=4 S...	[more]
A0A1S3BRU8	5.8e-62	95.04	fructokinase-1 OS=Cucumis melo OX=3656 GN=LOC103492613 PE=4 SV=1	[more]
A0A6J1H739	1.6e-43	71.07	fructokinase-1 OS=Cucurbita moschata OX=3662 GN=LOC111461046 PE=4 SV=1	[more]
A0A6J1KXH7	7.9e-43	70.25	uncharacterized protein LOC111498032 OS=Cucurbita maxima OX=3661 GN=LOC111498032...	[more]
A0A6J1DRF9	3.4e-38	61.54	fructokinase-1 OS=Momordica charantia OX=3673 GN=LOC111022867 PE=4 SV=1	[more]

BLAST of CSPI07G15720.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004146987.1	1.5e-64	100.00	fructokinase-1 [Cucumis sativus] >KAE8646296.1 hypothetical protein Csa_015704 [...	[more]
XP_008451263.1	1.2e-61	95.04	PREDICTED: fructokinase-1 [Cucumis melo]	[more]
XP_038898169.1	2.0e-56	88.43	fructokinase-1 [Benincasa hispida]	[more]
XP_023514771.1	3.3e-43	71.07	uncharacterized protein LOC111778983 [Cucurbita pepo subsp. pepo]	[more]
XP_022960246.1	3.3e-43	71.07	fructokinase-1 [Cucurbita moschata] >XP_022960247.1 fructokinase-1 [Cucurbita mo...	[more]

BLAST of CSPI07G15720.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G06730.1	6.6e-10	65.91	pfkB-like carbohydrate kinase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 101..123
None	No IPR available	PANTHER	PTHR47826	OS03G0164700 PROTEIN	coord: 22..121

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI07G15720	CSPI07G15720	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI07G15720.1.utr5p1	CSPI07G15720.1.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI07G15720.1.cds1	CSPI07G15720.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI07G15720.1	CSPI07G15720.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
molecular_function	GO:0016301	kinase activity