CSPI07G12470.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI07G12470.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Reverse transcriptase
Location	Chr7: 10751721 .. 10752335 (+)
Sequence length	615
RNA-Seq Expression	CSPI07G12470.1
Synteny	CSPI07G12470.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTATTATCATAAGAAAATAATAAGAGATATCAGCATCGTGGTTATTCTTCTTGTATTAGGGTTTTTGCGTAAATTTGTGTTCGTTCTATAGCTCTACTTTTAATATGGTATCAAAGCGGGATATCGAAGAGACCCTAGACAATCAAACACTTAATGGAACCCTAGTAGAAGGGACAACCATCGACATCAGTGCAGTCGTCACCGCCGCAGTTGGCATCTTCAGAACCCATCGGCCAGCCTTCCTGCCCAACCACAATCGTCTGGCAACCAGCAGCCTTCAGCTGCACAGGTCCCACACGCGCAGCCAATTTGGATTCCCTCATATGCGCTGCCTGGTCTTCATGCACTGCTGCCTGCGCCTACACCTTCACTGTATGCACCACCACACACACTGTCGCATATGTTACTGCACGTGCCATCTTCAGGACTTGCCCAGCCACCGACCTTGGTTCAGTCTTCTGCTGCAGGTGTGATTGCGCCGCCTCCTACTAGGTTAGTCGCAAACCCTAGTGGTTGCCCGGAGGTTGGAAATCCAGCCCAGTCGACCTTCCCAGCACAGTCTAAGTGCCCATGAGCAGGGTTCATCTCTCAGTATTGCTCAACATCAGCAGGAC

mRNA sequence

TTTATTATCATAAGAAAATAATAAGAGATATCAGCATCGTGGTTATTCTTCTTGTATTAGGGTTTTTGCGTAAATTTGTGTTCGTTCTATAGCTCTACTTTTAATATGGTATCAAAGCGGGATATCGAAGAGACCCTAGACAATCAAACACTTAATGGAACCCTAGTAGAAGGGACAACCATCGACATCAGTGCAGTCGTCACCGCCGCAGTTGGCATCTTCAGAACCCATCGGCCAGCCTTCCTGCCCAACCACAATCGTCTGGCAACCAGCAGCCTTCAGCTGCACAGGTCCCACACGCGCAGCCAATTTGGATTCCCTCATATGCGCTGCCTGGTCTTCATGCACTGCTGCCTGCGCCTACACCTTCACTGTATGCACCACCACACACACTGTCGCATATGTTACTGCACGTGCCATCTTCAGGACTTGCCCAGCCACCGACCTTGGTTCAGTCTTCTGCTGCAGGTGTGATTGCGCCGCCTCCTACTAGGTTAGTCGCAAACCCTAGTGGTTGCCCGGAGGTTGGAAATCCAGCCCAGTCGACCTTCCCAGCACAGTCTAAGTGCCCATGAGCAGGGTTCATCTCTCAGTATTGCTCAACATCAGCAGGAC

Coding sequence (CDS)

ATGGTATCAAAGCGGGATATCGAAGAGACCCTAGACAATCAAACACTTAATGGAACCCTAGTAGAAGGGACAACCATCGACATCAGTGCAGTCGTCACCGCCGCAGTTGGCATCTTCAGAACCCATCGGCCAGCCTTCCTGCCCAACCACAATCGTCTGGCAACCAGCAGCCTTCAGCTGCACAGGTCCCACACGCGCAGCCAATTTGGATTCCCTCATATGCGCTGCCTGGTCTTCATGCACTGCTGCCTGCGCCTACACCTTCACTGTATGCACCACCACACACACTGTCGCATATGTTACTGCACGTGCCATCTTCAGGACTTGCCCAGCCACCGACCTTGGTTCAGTCTTCTGCTGCAGGTGTGA

Protein sequence

MVSKRDIEETLDNQTLNGTLVEGTTIDISAVVTAAVGIFRTHRPAFLPNHNRLATSSLQLHRSHTRSQFGFPHMRCLVFMHCCLRLHLHCMHHHTHCRICYCTCHLQDLPSHRPWFSLLLQV*

Homology

[Close]

BLAST of CSPI07G12470.1 vs. ExPASy TrEMBL
Match: A0A5D3DIW2 (Beta-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G00370 PE=4 SV=1)

HSP 1 Score: 66.2 bits (160), Expect = 1.1e-07
Identity = 33/46 (71.74%), Postives = 35/46 (76.09%), Query Frame = 0


Query: 37 GIFRTHRPAFLPNHNRLATSSLQLHRSHTRSQFGFPHMRCLVFMHC 83
          GIF+TH PAFLP+ NR ATSSLQ HRS  R Q GFPHMR L  MHC
Sbjct: 13 GIFKTHWPAFLPSCNRPATSSLQPHRSLMRRQSGFPHMRHLALMHC 58

[Close]

BLAST of CSPI07G12470.1 vs. ExPASy TrEMBL
Match: A0A5D3C538 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold178G00080 PE=4 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 6.4e-05
Identity = 28/36 (77.78%), Postives = 32/36 (88.89%), Query Frame = 0


Query: 1  MVSKRDIEETLDNQTLNGTLVEGTTIDISAVVTAAV 37
          MVSKRDIE+TLDNQTL+GTL +GT +DISAVVT  V
Sbjct: 1  MVSKRDIEKTLDNQTLDGTLAKGTVVDISAVVTTVV 36

[Close]

BLAST of CSPI07G12470.1 vs. ExPASy TrEMBL
Match: A0A5A7UMP8 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold222G00070 PE=4 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 8.4e-05
Identity = 38/73 (52.05%), Postives = 43/73 (58.90%), Query Frame = 0


Query: 1  MVSKRDIEETLDNQTLNGTLVEGTTIDISAVVTAAV------------GIFRTHRPAFLP 60
          MVSK DIEET D+QTL+GTL EG  +DI+A V A V             +F TH    L 
Sbjct: 1  MVSKWDIEETQDDQTLDGTLAEGIVLDINAAVVATVDGKSAPPWMSGFDVFITH----LL 60

Query: 61 NHNRLATSSLQLH 62
          N NR ATSSLQ H
Sbjct: 61 NRNRPATSSLQPH 69

[Close]

BLAST of CSPI07G12470.1 vs. ExPASy TrEMBL
Match: A0A5D3BTH6 (Copia protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold29G00830 PE=4 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 2.4e-04
Identity = 37/73 (50.68%), Postives = 43/73 (58.90%), Query Frame = 0


Query: 1  MVSKRDIEETLDNQTLNGTLVEGTTIDISAVVTAAV------------GIFRTHRPAFLP 60
          MVS+ DIEET D+QTL+GTL EG  +DI+A V A V             +F TH    L 
Sbjct: 1  MVSQWDIEETQDDQTLDGTLAEGIVLDINAAVVATVDGKSAPPWMSGFDVFITH----LL 60

Query: 61 NHNRLATSSLQLH 62
          N NR ATSSLQ H
Sbjct: 61 NRNRPATSSLQPH 69

[Close]

BLAST of CSPI07G12470.1 vs. ExPASy TrEMBL
Match: A0A5A7V5P9 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold468G00320 PE=4 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 3.2e-04
Identity = 54/135 (40.00%), Postives = 69/135 (51.11%), Query Frame = 0


Query: 1   MVSKRDIEETLDNQTLNGTLVEGTTIDIS--AVVTAAVG------IFRTHRPAF---LPN 60
           MVS+ DIEETLD+Q L+GTLVEGT +DIS  AVV A +        +R   P      P+
Sbjct: 1   MVSEWDIEETLDDQALDGTLVEGTIVDISAAAVVDARISDAMDEWFWRLQNPLTSQPQPS 60

Query: 61  HNRLATSSLQLHR-SHTRSQFGFPHMRCLVFMHCCLRLHLH---CMHHHTHCRICYCTCH 120
            NR  ++   LH  S     +  P +    FM    RLHLH     +  THCR  +    
Sbjct: 61  DNRNHSAEQVLHTLSVWIYPYLLPSLSNQAFMCSHSRLHLHLPPSPNRFTHCRTRHRMHR 120

[Close]

BLAST of CSPI07G12470.1 vs. NCBI nr
Match: KAA0032653.1 (Beta-galactosidase [Cucumis melo var. makuwa] >TYK23533.1 Beta-galactosidase [Cucumis melo var. makuwa])

HSP 1 Score: 66.2 bits (160), Expect = 2.2e-07
Identity = 33/46 (71.74%), Postives = 35/46 (76.09%), Query Frame = 0


Query: 37 GIFRTHRPAFLPNHNRLATSSLQLHRSHTRSQFGFPHMRCLVFMHC 83
          GIF+TH PAFLP+ NR ATSSLQ HRS  R Q GFPHMR L  MHC
Sbjct: 13 GIFKTHWPAFLPSCNRPATSSLQPHRSLMRRQSGFPHMRHLALMHC 58

[Close]

BLAST of CSPI07G12470.1 vs. NCBI nr
Match: KAA0063757.1 (reverse transcriptase [Cucumis melo var. makuwa] >TYK05509.1 reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 57.0 bits (136), Expect = 1.3e-04
Identity = 28/36 (77.78%), Postives = 32/36 (88.89%), Query Frame = 0


Query: 1  MVSKRDIEETLDNQTLNGTLVEGTTIDISAVVTAAV 37
          MVSKRDIE+TLDNQTL+GTL +GT +DISAVVT  V
Sbjct: 1  MVSKRDIEKTLDNQTLDGTLAKGTVVDISAVVTTVV 36

[Close]

BLAST of CSPI07G12470.1 vs. NCBI nr
Match: KAA0055536.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 56.6 bits (135), Expect = 1.7e-04
Identity = 38/73 (52.05%), Postives = 43/73 (58.90%), Query Frame = 0


Query: 1  MVSKRDIEETLDNQTLNGTLVEGTTIDISAVVTAAV------------GIFRTHRPAFLP 60
          MVSK DIEET D+QTL+GTL EG  +DI+A V A V             +F TH    L 
Sbjct: 1  MVSKWDIEETQDDQTLDGTLAEGIVLDINAAVVATVDGKSAPPWMSGFDVFITH----LL 60

Query: 61 NHNRLATSSLQLH 62
          N NR ATSSLQ H
Sbjct: 61 NRNRPATSSLQPH 69

[Close]

BLAST of CSPI07G12470.1 vs. NCBI nr
Match: TYK01389.1 (Copia protein [Cucumis melo var. makuwa])

HSP 1 Score: 55.1 bits (131), Expect = 5.1e-04
Identity = 37/73 (50.68%), Postives = 43/73 (58.90%), Query Frame = 0


Query: 1  MVSKRDIEETLDNQTLNGTLVEGTTIDISAVVTAAV------------GIFRTHRPAFLP 60
          MVS+ DIEET D+QTL+GTL EG  +DI+A V A V             +F TH    L 
Sbjct: 1  MVSQWDIEETQDDQTLDGTLAEGIVLDINAAVVATVDGKSAPPWMSGFDVFITH----LL 60

Query: 61 NHNRLATSSLQLH 62
          N NR ATSSLQ H
Sbjct: 61 NRNRPATSSLQPH 69

[Close]

BLAST of CSPI07G12470.1 vs. NCBI nr
Match: KAA0062938.1 (uncharacterized protein E6C27_scaffold468G00320 [Cucumis melo var. makuwa])

HSP 1 Score: 54.7 bits (130), Expect = 6.6e-04
Identity = 54/135 (40.00%), Postives = 69/135 (51.11%), Query Frame = 0


Query: 1   MVSKRDIEETLDNQTLNGTLVEGTTIDIS--AVVTAAVG------IFRTHRPAF---LPN 60
           MVS+ DIEETLD+Q L+GTLVEGT +DIS  AVV A +        +R   P      P+
Sbjct: 1   MVSEWDIEETLDDQALDGTLVEGTIVDISAAAVVDARISDAMDEWFWRLQNPLTSQPQPS 60

Query: 61  HNRLATSSLQLHR-SHTRSQFGFPHMRCLVFMHCCLRLHLH---CMHHHTHCRICYCTCH 120
            NR  ++   LH  S     +  P +    FM    RLHLH     +  THCR  +    
Sbjct: 61  DNRNHSAEQVLHTLSVWIYPYLLPSLSNQAFMCSHSRLHLHLPPSPNRFTHCRTRHRMHR 120

The following BLAST results are available for this feature:

BLAST of CSPI07G12470.1 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI07G12470.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DIW2	1.1e-07	71.74	Beta-galactosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G0...	[more]
A0A5D3C538	6.4e-05	77.78	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold17...	[more]
A0A5A7UMP8	8.4e-05	52.05	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold22...	[more]
A0A5D3BTH6	2.4e-04	50.68	Copia protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold29G00830 P...	[more]
A0A5A7V5P9	3.2e-04	40.00	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]

BLAST of CSPI07G12470.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0032653.1	2.2e-07	71.74	Beta-galactosidase [Cucumis melo var. makuwa] >TYK23533.1 Beta-galactosidase [Cu...	[more]
KAA0063757.1	1.3e-04	77.78	reverse transcriptase [Cucumis melo var. makuwa] >TYK05509.1 reverse transcripta...	[more]
KAA0055536.1	1.7e-04	52.05	reverse transcriptase [Cucumis melo var. makuwa]	[more]
TYK01389.1	5.1e-04	50.68	Copia protein [Cucumis melo var. makuwa]	[more]
KAA0062938.1	6.6e-04	40.00	uncharacterized protein E6C27_scaffold468G00320 [Cucumis melo var. makuwa]	[more]

BLAST of CSPI07G12470.1 vs. NCBI nr


Match Name	E-value	Identity	Description

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI07G12470	CSPI07G12470	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI07G12470.1.utr5p1	CSPI07G12470.1.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI07G12470.1.cds1	CSPI07G12470.1.cds1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI07G12470.1.utr3p1	CSPI07G12470.1.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI07G12470.1	CSPI07G12470.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006278	RNA-dependent DNA biosynthetic process
molecular_function	GO:0003964	RNA-directed DNA polymerase activity