CSPI06G33040.1 (mRNA) Cucumber (PI 183967) v1

Overview
NameCSPI06G33040.1
TypemRNA
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionSulfate adenylyltransferase subunit 2
LocationChr6: 27595341 .. 27597551 (+)
Sequence length1032
RNA-Seq ExpressionCSPI06G33040.1
SyntenyCSPI06G33040.1
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GACGAAGCTTACTCTCTTTATATTTTCATTAGTTTGTTACCATAAAGTTATCCCTTTGAATACCCAAAACTAAACCCTGAGGCCAACGCTAACGTCTCTTCTCCGACTCTGACCATCACCTTTGAAAGCGGAGGCCGTGGTTTTTCGGCCGGGAGATGCAAAAACAATGCTTCAGGCTCTAAATCTAAGACCCCCACCTCCCATTCTCGCCCTATCCTTCTCAGTTTCCGATGACCCAACTTGTTCCCCCCTCCCATTACGTCGCCCTCGTAATCCAATGCACAATTGGGCGCTTTTGCAGTCCAAACTCAAGTGCAACGGCAGATTCTCATGCCTTTTCTCCGACAATCGAAAAGAGGTGCTGTGTGTTTCTTCTCTTTATTTGTTTCAACCTTCAACTGATTACAATTTGTAGCTTCTTCTTTTTCCTGTTCTGTTCGTTTTCAATAACCTAGTTAATTTTCTGAGATTGATATATCCGGTCCGATTTTCTCTACTTTTTCGAGGAAAGATAAATGCGTGGCCAAACTTTATGCAAGGGATTTGCGTTCTGTGGCTTACTTTTTGGGGGAAATTTTGAGAACATATGGAAGGAACACTGAGGTTGGGTAAATACTTCAAAGCTAGGATGGGAGATAGGATGATTTGGAGTTGGAACTTAATACCCATAACAAGCTTCAAATGGATGGAATGGGATATCTTTGTTTGGTATTGCTAGCTATAGCTATTTGTAGATTTATTAGAAAATTTTAGTGGATATTAATCTTCCTATACTTGAATTCTTTAACTTAGGAAGCCACTAATTTGCGCTTTCGTATGAAGAAATGTGGAAGCTGGTCTGCATGCACTCTTGTTTTTAGTACAATAGGCCGTGAAGTCATTGAGTTATGTTCACTTTAGCGATTGCGTGTACTCTGTTGATGCTGCCTTCAACTGTATCTGTCCGCTTTGGCTTTTACTAAATTTGGTCCTAAGAGGAAAAAAAGGGTCAATAAATCAAGTAGACTGGCTGCTGAAAAACTGAAGTATGAATTGTTCTAATCCTGTTTGAGAGAGGACTCAACTTGTTTCTATCTTTTTCAATTATTTTAAGGAAGCATCCTTCTATCCTGTATTTTTTTGTCCATGAGAATTATAAATGATCAACATTTATTCTTGGTAGCTGTAGTTGCCCAGTTACATAATTTTCCTTTTCCCAGGGCAGTGCCACTGTTGTTAGATATGCATATCCATCCCTTCACTTAACTCTCAAAAGAAGGAAAAGAAAAAAAAGAACAACAATGAGAATTGACTTTATGTTGTTATTTAACTTTCTGGATCCAATGTGCACCATATACAGACATGCAGTGCATTCTTCTTCATTATCGGGCCCAACAAAGTGTTTTTAGGAAATCTTGCTTAAATTTGCCTTCTCTCTTTGTTGACATCAGGAACAAGCAAGGAAGGCATTAGAAAGTGCACTTGGGGGAAAGAAAAATGAATTTGAGAAATGGAATAATGAAATAAAGAAAAGAGAGGAGGTTGGCGGTGGCAGTGGCAGTGGTGGAGGACGAGGAGGTTGGTTTGGATCAGGCGGATGGTTTGGTTGGTCAGATGACCAATTCTGGCCAGAAGCACAACAGACCAGTCTTGCTGTTCTTGGTATAATTGTGATGGTAAGTATTTTCCTTTATAATCCATCGTAGATTCAGGTTATAACTATTTTAGAATGCAATCTAACTAACTAATCTCTCATTTCACTGGTGCGAATTTTGAATAAAAGTATCTCCTAGTTGCAAAGGGTGAACTGTTGCTTGCTGTTGTTTTCAACCCACTGCTTTATGCTTTGCGCGGAACAAGAAATGGATTGACTTTTGTAACTTCAAAAATTTTGAGAAAGTCCTCTGCTAGTAATTATGCTGAGGTTGAGATGATTTCAAACAAAGATGTCTCTGCCAAAGATAGAGTTGCAAGGAAATGGGGGAGCGATTGATCTTTTTGACCTTCAAATAGGTTGCTATGTAATTTTTTGGAAAATGTTTGGCGTCTAGAAATAGTTCTTTTTTTTTTTTTTTTATGTGGATTACTTTATTTCATGTTAAAGAATAGTTTCCTTTCAAACCAGATGCTTGAGTTTGTTCCCTTCACCAAAAATCACCCCAAAAGTGAAATATATTGGATTGTATAATTACTCTTCAAACTTTGTTATATCTAATCTGACATTTATTTTCA

mRNA sequence

GACGAAGCTTACTCTCTTTATATTTTCATTAGTTTGTTACCATAAAGTTATCCCTTTGAATACCCAAAACTAAACCCTGAGGCCAACGCTAACGTCTCTTCTCCGACTCTGACCATCACCTTTGAAAGCGGAGGCCGTGGTTTTTCGGCCGGGAGATGCAAAAACAATGCTTCAGGCTCTAAATCTAAGACCCCCACCTCCCATTCTCGCCCTATCCTTCTCAGTTTCCGATGACCCAACTTGTTCCCCCCTCCCATTACGTCGCCCTCGTAATCCAATGCACAATTGGGCGCTTTTGCAGTCCAAACTCAAGTGCAACGGCAGATTCTCATGCCTTTTCTCCGACAATCGAAAAGAGGAACAAGCAAGGAAGGCATTAGAAAGTGCACTTGGGGGAAAGAAAAATGAATTTGAGAAATGGAATAATGAAATAAAGAAAAGAGAGGAGGTTGGCGGTGGCAGTGGCAGTGGTGGAGGACGAGGAGGTTGGTTTGGATCAGGCGGATGGTTTGGTTGGTCAGATGACCAATTCTGGCCAGAAGCACAACAGACCAGTCTTGCTGTTCTTGGTATAATTGTGATGTATCTCCTAGTTGCAAAGGGTGAACTGTTGCTTGCTGTTGTTTTCAACCCACTGCTTTATGCTTTGCGCGGAACAAGAAATGGATTGACTTTTGTAACTTCAAAAATTTTGAGAAAGTCCTCTGCTAGTAATTATGCTGAGGTTGAGATGATTTCAAACAAAGATGTCTCTGCCAAAGATAGAGTTGCAAGGAAATGGGGGAGCGATTGATCTTTTTGACCTTCAAATAGGTTGCTATGTAATTTTTTGGAAAATGTTTGGCGTCTAGAAATAGTTCTTTTTTTTTTTTTTTTATGTGGATTACTTTATTTCATGTTAAAGAATAGTTTCCTTTCAAACCAGATGCTTGAGTTTGTTCCCTTCACCAAAAATCACCCCAAAAGTGAAATATATTGGATTGTATAATTACTCTTCAAACTTTGTTATATCTAATCTGACATTTATTTTCA

Coding sequence (CDS)

ATGCTTCAGGCTCTAAATCTAAGACCCCCACCTCCCATTCTCGCCCTATCCTTCTCAGTTTCCGATGACCCAACTTGTTCCCCCCTCCCATTACGTCGCCCTCGTAATCCAATGCACAATTGGGCGCTTTTGCAGTCCAAACTCAAGTGCAACGGCAGATTCTCATGCCTTTTCTCCGACAATCGAAAAGAGGAACAAGCAAGGAAGGCATTAGAAAGTGCACTTGGGGGAAAGAAAAATGAATTTGAGAAATGGAATAATGAAATAAAGAAAAGAGAGGAGGTTGGCGGTGGCAGTGGCAGTGGTGGAGGACGAGGAGGTTGGTTTGGATCAGGCGGATGGTTTGGTTGGTCAGATGACCAATTCTGGCCAGAAGCACAACAGACCAGTCTTGCTGTTCTTGGTATAATTGTGATGTATCTCCTAGTTGCAAAGGGTGAACTGTTGCTTGCTGTTGTTTTCAACCCACTGCTTTATGCTTTGCGCGGAACAAGAAATGGATTGACTTTTGTAACTTCAAAAATTTTGAGAAAGTCCTCTGCTAGTAATTATGCTGAGGTTGAGATGATTTCAAACAAAGATGTCTCTGCCAAAGATAGAGTTGCAAGGAAATGGGGGAGCGATTGA

Protein sequence

MLQALNLRPPPPILALSFSVSDDPTCSPLPLRRPRNPMHNWALLQSKLKCNGRFSCLFSDNRKEEQARKALESALGGKKNEFEKWNNEIKKREEVGGGSGSGGGRGGWFGSGGWFGWSDDQFWPEAQQTSLAVLGIIVMYLLVAKGELLLAVVFNPLLYALRGTRNGLTFVTSKILRKSSASNYAEVEMISNKDVSAKDRVARKWGSD*
Homology
BLAST of CSPI06G33040.1 vs. ExPASy TrEMBL
Match: A0A1S3B0A1 (uncharacterized protein LOC103484656 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484656 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 5.8e-99
Identity = 191/208 (91.83%), Postives = 196/208 (94.23%), Query Frame = 0

Query: 1   MLQALNLRPPPPILALSFSVSDDPTCSPLPLRRPRNPMHNWALLQSKLKCNGRFSCLFSD 60
           MLQALNLR  PPILALSFSVSDDPTCS LPL RPRNP HNWALL S LKCNGRFSCLFS+
Sbjct: 1   MLQALNLRTSPPILALSFSVSDDPTCSALPLLRPRNPTHNWALLLSNLKCNGRFSCLFSN 60

Query: 61  NRKEEQARKALESALGGKKNEFEKWNNEIKKREEVGGGSGSGGGRGGWFGSGGWFGWSDD 120
           NR+EEQARKALESALGGKKNEFEKWNNEIKKREEVGG  GSGGGRGGWFGSGGWFGWSDD
Sbjct: 61  NRREEQARKALESALGGKKNEFEKWNNEIKKREEVGG--GSGGGRGGWFGSGGWFGWSDD 120

Query: 121 QFWPEAQQTSLAVLGIIVMYLLVAKGELLLAVVFNPLLYALRGTRNGLTFVTSKILRKSS 180
           QFWPEAQQTSLAV GIIVMYL+VAKGELLLAV+FNPLLYALRGTRNGLTFVTSK LRKSS
Sbjct: 121 QFWPEAQQTSLAVFGIIVMYLIVAKGELLLAVIFNPLLYALRGTRNGLTFVTSKFLRKSS 180

Query: 181 ASNYAEVEMISNKDVSAKDRVARKWGSD 209
           ASNYAEVE ISNKDV+AKDRVARKWGSD
Sbjct: 181 ASNYAEVEEISNKDVTAKDRVARKWGSD 206

BLAST of CSPI06G33040.1 vs. ExPASy TrEMBL
Match: A0A1S3AZU3 (uncharacterized protein LOC103484656 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484656 PE=4 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.4e-97
Identity = 191/209 (91.39%), Postives = 196/209 (93.78%), Query Frame = 0

Query: 1   MLQALNLRPPPPILALSFSVSDDPTCSPLPLRRPRNPMHNWALLQSKLKCNGRFSCLFSD 60
           MLQALNLR  PPILALSFSVSDDPTCS LPL RPRNP HNWALL S LKCNGRFSCLFS+
Sbjct: 1   MLQALNLRTSPPILALSFSVSDDPTCSALPLLRPRNPTHNWALLLSNLKCNGRFSCLFSN 60

Query: 61  NRKE-EQARKALESALGGKKNEFEKWNNEIKKREEVGGGSGSGGGRGGWFGSGGWFGWSD 120
           NR+E EQARKALESALGGKKNEFEKWNNEIKKREEVGG  GSGGGRGGWFGSGGWFGWSD
Sbjct: 61  NRREQEQARKALESALGGKKNEFEKWNNEIKKREEVGG--GSGGGRGGWFGSGGWFGWSD 120

Query: 121 DQFWPEAQQTSLAVLGIIVMYLLVAKGELLLAVVFNPLLYALRGTRNGLTFVTSKILRKS 180
           DQFWPEAQQTSLAV GIIVMYL+VAKGELLLAV+FNPLLYALRGTRNGLTFVTSK LRKS
Sbjct: 121 DQFWPEAQQTSLAVFGIIVMYLIVAKGELLLAVIFNPLLYALRGTRNGLTFVTSKFLRKS 180

Query: 181 SASNYAEVEMISNKDVSAKDRVARKWGSD 209
           SASNYAEVE ISNKDV+AKDRVARKWGSD
Sbjct: 181 SASNYAEVEEISNKDVTAKDRVARKWGSD 207

BLAST of CSPI06G33040.1 vs. ExPASy TrEMBL
Match: A0A5D3CM52 (Sulfate adenylyltransferase subunit 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006010 PE=4 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.6e-88
Identity = 192/291 (65.98%), Postives = 197/291 (67.70%), Query Frame = 0

Query: 1   MLQALNLRPPPPILALSFSVSDDPTCSPLPLRRPRNPMHNWALLQSKLKCNGRFSCLFSD 60
           MLQALNLR  PPILALSFSVSDDPTCS LPL RPRNP HNWALL S LKCNGRFSCLFS+
Sbjct: 1   MLQALNLRTAPPILALSFSVSDDPTCSALPLLRPRNPTHNWALLLSNLKCNGRFSCLFSN 60

Query: 61  NRKE-------------------------------------------------------- 120
           NR+E                                                        
Sbjct: 61  NRREVLCFSSLYLFQPSTDYNLLLVRFPILFVFNNLVNFLRLILSGPIFSTFSRKDKWVA 120

Query: 121 ---------------------------EQARKALESALGGKKNEFEKWNNEIKKREEVGG 180
                                      EQARKALESALGGKKNEFEKWNNEIKKREEVGG
Sbjct: 121 KLYAMDLRSVAYFLGANLRTYGRTTEQEQARKALESALGGKKNEFEKWNNEIKKREEVGG 180

Query: 181 GSGSGGGRGGWFGSGGWFGWSDDQFWPEAQQTSLAVLGIIVMYLLVAKGELLLAVVFNPL 209
             GSGGGRGGWFGSGGWFGWSDDQFWPEAQQTSLAV GIIVMYL+VAKGELLLAV+FNPL
Sbjct: 181 --GSGGGRGGWFGSGGWFGWSDDQFWPEAQQTSLAVFGIIVMYLIVAKGELLLAVIFNPL 240

BLAST of CSPI06G33040.1 vs. ExPASy TrEMBL
Match: A0A6J1GG46 (uncharacterized protein LOC111453631 OS=Cucurbita moschata OX=3662 GN=LOC111453631 PE=4 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 1.3e-82
Identity = 170/212 (80.19%), Postives = 181/212 (85.38%), Query Frame = 0

Query: 1   MLQALNLRPPPPILALSFSVSDDPTCSPLPLRRPRNPMHNWALLQSKLKCNGRFSCLFSD 60
           MLQ LNL P    LALS SVSDDPT S LPL RPRN  H WALLQSKLKCN RFSCLFSD
Sbjct: 1   MLQVLNLSPSSRSLALSTSVSDDPTRSGLPLLRPRNATHEWALLQSKLKCNSRFSCLFSD 60

Query: 61  NRKEEQARKALESALGGKKNEFEKWNNEIKKREEVGGGSGSGGGRGGWFGSGGWFGWSDD 120
           NR+EEQARKALESALGGKKNEFEKWNNEIKKREE+ G    GGGRGGWFGSGGWFGWSDD
Sbjct: 61  NRREEQARKALESALGGKKNEFEKWNNEIKKREEMAG--DGGGGRGGWFGSGGWFGWSDD 120

Query: 121 QFWPEAQQTSLAVLGIIVMYLLVAKGELLLAVVFNPLLYALRGTRNGLTFVTSKILRKSS 180
           QFWPEAQQTSLAVLGIIVMYL+VAKG +L+AV+ NPLLYALRGTRNGLT VTSKILRK+ 
Sbjct: 121 QFWPEAQQTSLAVLGIIVMYLIVAKGGMLVAVIINPLLYALRGTRNGLTLVTSKILRKNP 180

Query: 181 ASNYAEVEMISNKD----VSAKDRVARKWGSD 209
           +SN AE + ISN+D    VSAKDRVARKW +D
Sbjct: 181 SSNDAEFDEISNEDVSGHVSAKDRVARKWRND 210

BLAST of CSPI06G33040.1 vs. ExPASy TrEMBL
Match: A0A6J1BSB9 (uncharacterized protein LOC111005307 OS=Momordica charantia OX=3673 GN=LOC111005307 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.9e-81
Identity = 165/212 (77.83%), Postives = 181/212 (85.38%), Query Frame = 0

Query: 1   MLQALNLRPPPPILALSFSVSDDPTCSPLPLRRPRNPMHNWALLQSKLKCNGRFSCLFSD 60
           ML+ LNL PPPP      S+ D+ T S +P  RPRN  HNWA LQ+KLKCN RFSCLFSD
Sbjct: 1   MLRILNLTPPPPT-----SIPDESTRSGVPSIRPRNSAHNWARLQAKLKCNARFSCLFSD 60

Query: 61  NRKEEQARKALESALGGKKNEFEKWNNEIKKREEVGGGSGSGGGRGGWFGSGGWFGWSDD 120
           NR+EEQARKALESALG KKNEFEKWNNEIKKREE+GG  G  GG+GGWFGSGGWFGWSDD
Sbjct: 61  NRREEQARKALESALGEKKNEFEKWNNEIKKREEMGG--GGSGGQGGWFGSGGWFGWSDD 120

Query: 121 QFWPEAQQTSLAVLGIIVMYLLVAKGELLLAVVFNPLLYALRGTRNGLTFVTSKILRKSS 180
            FWPEAQQTSLAVLGIIVMYL+VAKGELLLAV+FNPLLYALRGTRNGLTF+TSKILRKSS
Sbjct: 121 HFWPEAQQTSLAVLGIIVMYLIVAKGELLLAVIFNPLLYALRGTRNGLTFITSKILRKSS 180

Query: 181 ASNYAEVEMISNKD----VSAKDRVARKWGSD 209
           A NYAE + ISN++    VSAK++VARKWGSD
Sbjct: 181 AGNYAEFDEISNREVSGHVSAKEKVARKWGSD 205

BLAST of CSPI06G33040.1 vs. NCBI nr
Match: XP_004134798.1 (uncharacterized protein LOC101207146 [Cucumis sativus] >KAE8647643.1 hypothetical protein Csa_002771 [Cucumis sativus])

HSP 1 Score: 407.9 bits (1047), Expect = 5.2e-110
Identity = 208/208 (100.00%), Postives = 208/208 (100.00%), Query Frame = 0

Query: 1   MLQALNLRPPPPILALSFSVSDDPTCSPLPLRRPRNPMHNWALLQSKLKCNGRFSCLFSD 60
           MLQALNLRPPPPILALSFSVSDDPTCSPLPLRRPRNPMHNWALLQSKLKCNGRFSCLFSD
Sbjct: 1   MLQALNLRPPPPILALSFSVSDDPTCSPLPLRRPRNPMHNWALLQSKLKCNGRFSCLFSD 60

Query: 61  NRKEEQARKALESALGGKKNEFEKWNNEIKKREEVGGGSGSGGGRGGWFGSGGWFGWSDD 120
           NRKEEQARKALESALGGKKNEFEKWNNEIKKREEVGGGSGSGGGRGGWFGSGGWFGWSDD
Sbjct: 61  NRKEEQARKALESALGGKKNEFEKWNNEIKKREEVGGGSGSGGGRGGWFGSGGWFGWSDD 120

Query: 121 QFWPEAQQTSLAVLGIIVMYLLVAKGELLLAVVFNPLLYALRGTRNGLTFVTSKILRKSS 180
           QFWPEAQQTSLAVLGIIVMYLLVAKGELLLAVVFNPLLYALRGTRNGLTFVTSKILRKSS
Sbjct: 121 QFWPEAQQTSLAVLGIIVMYLLVAKGELLLAVVFNPLLYALRGTRNGLTFVTSKILRKSS 180

Query: 181 ASNYAEVEMISNKDVSAKDRVARKWGSD 209
           ASNYAEVEMISNKDVSAKDRVARKWGSD
Sbjct: 181 ASNYAEVEMISNKDVSAKDRVARKWGSD 208

BLAST of CSPI06G33040.1 vs. NCBI nr
Match: XP_008440069.1 (PREDICTED: uncharacterized protein LOC103484656 isoform X2 [Cucumis melo])

HSP 1 Score: 370.2 bits (949), Expect = 1.2e-98
Identity = 191/208 (91.83%), Postives = 196/208 (94.23%), Query Frame = 0

Query: 1   MLQALNLRPPPPILALSFSVSDDPTCSPLPLRRPRNPMHNWALLQSKLKCNGRFSCLFSD 60
           MLQALNLR  PPILALSFSVSDDPTCS LPL RPRNP HNWALL S LKCNGRFSCLFS+
Sbjct: 1   MLQALNLRTSPPILALSFSVSDDPTCSALPLLRPRNPTHNWALLLSNLKCNGRFSCLFSN 60

Query: 61  NRKEEQARKALESALGGKKNEFEKWNNEIKKREEVGGGSGSGGGRGGWFGSGGWFGWSDD 120
           NR+EEQARKALESALGGKKNEFEKWNNEIKKREEVGG  GSGGGRGGWFGSGGWFGWSDD
Sbjct: 61  NRREEQARKALESALGGKKNEFEKWNNEIKKREEVGG--GSGGGRGGWFGSGGWFGWSDD 120

Query: 121 QFWPEAQQTSLAVLGIIVMYLLVAKGELLLAVVFNPLLYALRGTRNGLTFVTSKILRKSS 180
           QFWPEAQQTSLAV GIIVMYL+VAKGELLLAV+FNPLLYALRGTRNGLTFVTSK LRKSS
Sbjct: 121 QFWPEAQQTSLAVFGIIVMYLIVAKGELLLAVIFNPLLYALRGTRNGLTFVTSKFLRKSS 180

Query: 181 ASNYAEVEMISNKDVSAKDRVARKWGSD 209
           ASNYAEVE ISNKDV+AKDRVARKWGSD
Sbjct: 181 ASNYAEVEEISNKDVTAKDRVARKWGSD 206

BLAST of CSPI06G33040.1 vs. NCBI nr
Match: XP_008440068.1 (PREDICTED: uncharacterized protein LOC103484656 isoform X1 [Cucumis melo])

HSP 1 Score: 365.5 bits (937), Expect = 3.0e-97
Identity = 191/209 (91.39%), Postives = 196/209 (93.78%), Query Frame = 0

Query: 1   MLQALNLRPPPPILALSFSVSDDPTCSPLPLRRPRNPMHNWALLQSKLKCNGRFSCLFSD 60
           MLQALNLR  PPILALSFSVSDDPTCS LPL RPRNP HNWALL S LKCNGRFSCLFS+
Sbjct: 1   MLQALNLRTSPPILALSFSVSDDPTCSALPLLRPRNPTHNWALLLSNLKCNGRFSCLFSN 60

Query: 61  NRKE-EQARKALESALGGKKNEFEKWNNEIKKREEVGGGSGSGGGRGGWFGSGGWFGWSD 120
           NR+E EQARKALESALGGKKNEFEKWNNEIKKREEVGG  GSGGGRGGWFGSGGWFGWSD
Sbjct: 61  NRREQEQARKALESALGGKKNEFEKWNNEIKKREEVGG--GSGGGRGGWFGSGGWFGWSD 120

Query: 121 DQFWPEAQQTSLAVLGIIVMYLLVAKGELLLAVVFNPLLYALRGTRNGLTFVTSKILRKS 180
           DQFWPEAQQTSLAV GIIVMYL+VAKGELLLAV+FNPLLYALRGTRNGLTFVTSK LRKS
Sbjct: 121 DQFWPEAQQTSLAVFGIIVMYLIVAKGELLLAVIFNPLLYALRGTRNGLTFVTSKFLRKS 180

Query: 181 SASNYAEVEMISNKDVSAKDRVARKWGSD 209
           SASNYAEVE ISNKDV+AKDRVARKWGSD
Sbjct: 181 SASNYAEVEEISNKDVTAKDRVARKWGSD 207

BLAST of CSPI06G33040.1 vs. NCBI nr
Match: XP_038881277.1 (uncharacterized protein LOC120072833 [Benincasa hispida])

HSP 1 Score: 346.3 bits (887), Expect = 1.9e-91
Identity = 181/208 (87.02%), Postives = 189/208 (90.87%), Query Frame = 0

Query: 1   MLQALNLRPPPPILALSFSVSDDPTCSPLPLRRPRNPMHNWALLQSKLKCNGRFSCLFSD 60
           MLQ LNLRPP PILALS S+S D TCS L L RPRN  HNWALLQS LKCNGRFSCLF D
Sbjct: 1   MLQVLNLRPPTPILALSTSISGDLTCSALLLLRPRNATHNWALLQSNLKCNGRFSCLFPD 60

Query: 61  NRKEEQARKALESALGGKKNEFEKWNNEIKKREEVGGGSGSGGGRGGWFGSGGWFGWSDD 120
           NR+EEQARKALESALGGKKNEFEKWNNEIKKREE+GG  G  GGRGGWFGSG WFGWSDD
Sbjct: 61  NRREEQARKALESALGGKKNEFEKWNNEIKKREEMGG--GGSGGRGGWFGSGRWFGWSDD 120

Query: 121 QFWPEAQQTSLAVLGIIVMYLLVAKGELLLAVVFNPLLYALRGTRNGLTFVTSKILRKSS 180
           QFWPEAQQTSLAVLGIIVMYL+VAKGELLLAVVFNPLLYALRGTRNGLTFVTSKILR +S
Sbjct: 121 QFWPEAQQTSLAVLGIIVMYLIVAKGELLLAVVFNPLLYALRGTRNGLTFVTSKILRNTS 180

Query: 181 ASNYAEVEMISNKDVSAKDRVARKWGSD 209
           ASNYAEVE ISNK+VSAK+RVA+KWGSD
Sbjct: 181 ASNYAEVEDISNKEVSAKERVAKKWGSD 206

BLAST of CSPI06G33040.1 vs. NCBI nr
Match: TYK13003.1 (Sulfate adenylyltransferase subunit 2 [Cucumis melo var. makuwa])

HSP 1 Score: 335.5 bits (859), Expect = 3.3e-88
Identity = 192/291 (65.98%), Postives = 197/291 (67.70%), Query Frame = 0

Query: 1   MLQALNLRPPPPILALSFSVSDDPTCSPLPLRRPRNPMHNWALLQSKLKCNGRFSCLFSD 60
           MLQALNLR  PPILALSFSVSDDPTCS LPL RPRNP HNWALL S LKCNGRFSCLFS+
Sbjct: 1   MLQALNLRTAPPILALSFSVSDDPTCSALPLLRPRNPTHNWALLLSNLKCNGRFSCLFSN 60

Query: 61  NRKE-------------------------------------------------------- 120
           NR+E                                                        
Sbjct: 61  NRREVLCFSSLYLFQPSTDYNLLLVRFPILFVFNNLVNFLRLILSGPIFSTFSRKDKWVA 120

Query: 121 ---------------------------EQARKALESALGGKKNEFEKWNNEIKKREEVGG 180
                                      EQARKALESALGGKKNEFEKWNNEIKKREEVGG
Sbjct: 121 KLYAMDLRSVAYFLGANLRTYGRTTEQEQARKALESALGGKKNEFEKWNNEIKKREEVGG 180

Query: 181 GSGSGGGRGGWFGSGGWFGWSDDQFWPEAQQTSLAVLGIIVMYLLVAKGELLLAVVFNPL 209
             GSGGGRGGWFGSGGWFGWSDDQFWPEAQQTSLAV GIIVMYL+VAKGELLLAV+FNPL
Sbjct: 181 --GSGGGRGGWFGSGGWFGWSDDQFWPEAQQTSLAVFGIIVMYLIVAKGELLLAVIFNPL 240

BLAST of CSPI06G33040.1 vs. TAIR 10
Match: AT5G20130.1 (unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 180.6 bits (457), Expect = 1.3e-45
Identity = 104/180 (57.78%), Postives = 129/180 (71.67%), Query Frame = 0

Query: 33  RPRNPMHNWALLQSKLKCNGRFSCLFS-DNRKEEQARKALESALGGKKNEFEKWNNEIKK 92
           R  +P   ++L  S  K  GRFSCLFS  N++EEQARK+LESALGGKKNEFEKW+ EIKK
Sbjct: 25  RTLDPNSVFSLRTSITKSKGRFSCLFSGGNQREEQARKSLESALGGKKNEFEKWDKEIKK 84

Query: 93  REEVGGGS-GSGGGRGGWFGSGGWFGWSDDQFWPEAQQTSLAVLGIIVMYLLVAKGELLL 152
           REE GGG+ G GGG GGWFG GGWF  S D FW EAQQ +  +L I+ +Y++VAKGE++ 
Sbjct: 85  REESGGGNGGKGGGGGGWFGGGGWF--SGDHFWKEAQQIAFTLLAILAVYMVVAKGEVMA 144

Query: 153 AVVFNPLLYALRGTRNGLTFVTSKILRK--SSASNYAEVEMISNKDVSAKDRVARKWGSD 209
           A V NPLLYALRGTR GL+ ++SK++ +  S  S     EM   +  +AK+ V RKWGSD
Sbjct: 145 AFVLNPLLYALRGTREGLSSLSSKLMGREASKVSGDNSEEMWKKEGSTAKESVVRKWGSD 202

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A1S3B0A15.8e-9991.83uncharacterized protein LOC103484656 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A1S3AZU31.4e-9791.39uncharacterized protein LOC103484656 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A5D3CM521.6e-8865.98Sulfate adenylyltransferase subunit 2 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A6J1GG461.3e-8280.19uncharacterized protein LOC111453631 OS=Cucurbita moschata OX=3662 GN=LOC1114536... [more]
A0A6J1BSB91.9e-8177.83uncharacterized protein LOC111005307 OS=Momordica charantia OX=3673 GN=LOC111005... [more]
Match NameE-valueIdentityDescription
XP_004134798.15.2e-110100.00uncharacterized protein LOC101207146 [Cucumis sativus] >KAE8647643.1 hypothetica... [more]
XP_008440069.11.2e-9891.83PREDICTED: uncharacterized protein LOC103484656 isoform X2 [Cucumis melo][more]
XP_008440068.13.0e-9791.39PREDICTED: uncharacterized protein LOC103484656 isoform X1 [Cucumis melo][more]
XP_038881277.11.9e-9187.02uncharacterized protein LOC120072833 [Benincasa hispida][more]
TYK13003.13.3e-8865.98Sulfate adenylyltransferase subunit 2 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT5G20130.11.3e-4557.78unknown protein; Has 30201 Blast hits to 17322 proteins in 780 species: Archae -... [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 68..88
NoneNo IPR availablePANTHERPTHR36393SULFATE ADENYLYLTRANSFERASE SUBUNITcoord: 1..208

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
CSPI06G33040CSPI06G33040gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI06G33040.1.utr5p1CSPI06G33040.1.utr5p1five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI06G33040.1.cds1CSPI06G33040.1.cds1CDS
CSPI06G33040.1.cds2CSPI06G33040.1.cds2CDS
CSPI06G33040.1.cds3CSPI06G33040.1.cds3CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
CSPI06G33040.1.utr3p1CSPI06G33040.1.utr3p1three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
CSPI06G33040.1CSPI06G33040.1-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016740 transferase activity