CSPI06G25030.2 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI06G25030.2
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Threonine dehydratase
Location	Chr6: 22323370 .. 22326475 (+)
Sequence length	1820
RNA-Seq Expression	CSPI06G25030.2
Synteny	CSPI06G25030.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAAAAGGATGGAATAGAAACAGGGAAGTAAATTCCATGGTTTTGTTTTGATTGGCGAAAACCAAAGGAATCCTTCGTTTCTCATTTCTCCCCATCAAATTCTTAGCTGCACTTAACCACACCCCAAAAAACGAAAACAAATACAAAACTACAGCACAACTTTATTGGGTGGGAGCAGGTGCCGTTCCTCTCCGCCGCCACCCCCCTCTCTTCCTTTTCCCCTCCTCCGATCGCACCCCTCTTCTCTCAACTCCTATGGATGCTCTTCTTCTCAACGCACCCACTTCACACCCAATTCCTCGTAAATCCTTCCTTCCATCCCCTCCTTCCTTCTTCAATGGCTCCTCCATTTCGATTGATGACAATCGAAACAGAGTTCGACTTACCAGGCCTCCAATCATCCTAGCCACGCTTTCAAAGCACGCCCTTGATATCCCTCCTAATTCTGTATCCAACACTTCGGTTCCGACTCCTGGAGATGCATTGGCGCCTACCCCTTTGCCTCCTCGCAAAAGGGTATCGGCGGATTCTCTGCAGTTCCCGCCTGGCTATCTTGGCGCTGTTCCGGACCGTTCCGGTTCTGATAATGGCGAGGATAGTGTTGACGCCATGGAGTATTTGACTAGAATATTGGGTTCGAAGGTTTATGATGTGGCTATTGAATCGCCCTTGCAGCTTGCGCCGACGTTGTCTGAACGGTTTGGGGTTAACATCTGGCTGAAGAGGGAGGACTTGCAGCCTGTAAGTGAATTTTGGGTAGTTTTCATTTTTATTAATCTATTGGTTTGAAAGTTGGTATTTCTTTGGTCGTTTGTTCAAGGATTCTGTGTGAACTAGTACCCATGTTTTCAATTTAAACTTTTGTTTAACATGTTGCGTTCACTGTTGAGCATTCATCGACTTCTTTTTAGAATATCAAATTGCGGCTCGACGGTCTTTTGATTTCAACATCAGATGAAGACGGCTGATTCTGCTGATAAGGACTAATGGACGATTTTGCAAAGTAGTCATGTTTTTTAACCCCCGTTTTTATTTATTTATTTATTTATTTGTAGGTGTTCTCATTCAAGCTCCGAGGAGCTTATAATATGATGGCCAAACTTCCAAAAGAACAGTTGGAAAAAGGAGTTATATGCTCTTCAGCTGGAAATCATGCTCAAGGGGTTGCATTAGCTGCTAAGCGATTAAGATGTAATGCTGTTATTGCTATGCCAGTTACTACGCCTGAAATTAAGGTGCAATAATTTCTTCAGCTATGGGTTCTCTCTTTTTGACCTGGTGTTTCATGCGGATAACAATGTTGTTGGTTTGCTGATGAAAATGAGAACTTTTAACGTTTCAGTGGCAATCAGTTCAAAAATTGGGTGCGACGGTTGTTCTTGTTGGAGATTCATACGATGAGGCACAAGCATATGCTAAAAAGCGGAGCGTAGAGGAAGGCCGCACATTCATTCCCCCTTTCGATCACCCTGATGTTATAGCAGGGCAGGGTACAGTTGGGATGGAAATTGTGCGTCAAATAAAAGGTCCAGTGCATGCAATTTTTGTACCAGTTGGGGGCGGTGGTCTAATAGCTGGTATTGCTGCTTATGTCAAAAGGGTTTCTCCTGAGGTCTGTTGGTCTCTGTTGGAGTGGATTTTTGTGTTAAGCTTTCTTATCCTAACATTATTTTTTTAGTTCAACAATAAGTGGGGGTGGGTATTCGAACAGCCTTTTGATCAAGCTTATAATGTGTTTTAACCAGTTGAGCTATGCTCATTTTTTGTTATCTGTTTACAGTACCTGCTTCATTTGGCCATAAGATTTTTATTAATCTTGAATGGGATGAAGTGTCAGAATTATGGGGATTCTTGATGTTACACTATTTCTTAATATATCTATTCTTCCCTCTAAGGTGCCAGACTTGAAATGTTATCTATTATGGAAATATTAATTGGTTTTATTTTTCACGTCTTTCATACAGGTAAAGATCATTGGGGTAGAGCCATCTGATGCAAATGCTATGGCATTATCATTATGTCATGGCCAACGAGTAATATTGGACAAGGCTGGAGGCTTTGCTGATGGTGTAGCAGTTAAAGAAGTCGGTGAAGAGACTTTTCGCCTTTGCAAAGATCTCATGGATGGTGTAGTTCTTGTTAGCCGTGATGCTATATGTGCCTCGATAAAGGTATGAGTTTCTTTTCTCCCTTTCATGCACACGTGGCATTGTCTTATAACATAAAAAGATTAACTTCTCATTTGTTGAAGCATATGACATTTCCCAGTCGATCTTTATGCTTCCATAGAAAGAATCACAATGAAGATTCAATTGAATGAACGAAAGGCTATTGCTAATTTTGATATTCTTAACACCTAATATAGATATATGCTCAAACTATAACTGGTCTTTTTCTCCTCCTTTAAATGGTATTTGTAAAAATGATGTGATTTTTCTTGTGGAACTTGTCTTCAACATTCTTCAAGCAGTGGTTTTAGCAGAGGTTTTTTATGTTTGTTATTATGTCACAGGATATGTTTGAGGAGAAGAGAAGCATCTTGGAACCTGCAGGTGCTCTTTCTCTTGCTGGAGCTGAGGCCTACTGCAAATATTATGGCTTGAAGGGAGAAAATGTTGTGGTAATAACCAGTGGTGCAAATATGAACTTTGACAAACTGAGTATAGTTACTGAACTTGCCAATGTTGGTCGTGAGAAAGAGGCTGTGCTTGCAACTATATTGCCGGAGACACCTGGAAGCTTTAAAAAATTTTGTGAACTGGTTTGTTTATTAGTATTTCTAAGTTTTCTTGCTAAACATCGTTCTTGGTTGATTTTCATAGTTTTTAGAATGAATGCTATAAACAGGGACTTTGGATTTTCAGGTTGGGCCTATGAACATTACAGAATTCAAGTACAGATATAGTTCTGAAAAAGAAGCTGTTGTTCTTTATAGGTAAGTGCTTATCAAGCTATTGCCAATCTGAATTAAAACTTTTTTTCTAGTTTTTCAGTAGCTTTATAGGTAAGTGCTTATCAAGCTATTGGTAAGGAAAATTTAGAAATAAAAAACAGTGATTTAATGAGAATATTAAAACAAAAGCAAGGGAAACGGAAAGAGGAGTT

mRNA sequence

AGAAAAAGGATGGAATAGAAACAGGGAAGTAAATTCCATGGTTTTGTTTTGATTGGCGAAAACCAAAGGAATCCTTCGTTTCTCATTTCTCCCCATCAAATTCTTAGCTGCACTTAACCACACCCCAAAAAACGAAAACAAATACAAAACTACAGCACAACTTTATTGGGTGGGAGCAGGTGCCGTTCCTCTCCGCCGCCACCCCCCTCTCTTCCTTTTCCCCTCCTCCGATCGCACCCCTCTTCTCTCAACTCCTATGGATGCTCTTCTTCTCAACGCACCCACTTCACACCCAATTCCTCGTAAATCCTTCCTTCCATCCCCTCCTTCCTTCTTCAATGGCTCCTCCATTTCGATTGATGACAATCGAAACAGAGTTCGACTTACCAGGCCTCCAATCATCCTAGCCACGCTTTCAAAGCACGCCCTTGATATCCCTCCTAATTCTGTATCCAACACTTCGGTTCCGACTCCTGGAGATGCATTGGCGCCTACCCCTTTGCCTCCTCGCAAAAGGGTATCGGCGGATTCTCTGCAGTTCCCGCCTGGCTATCTTGGCGCTGTTCCGGACCGTTCCGGTTCTGATAATGGCGAGGATAGTGTTGACGCCATGGAGTATTTGACTAGAATATTGGGTTCGAAGGTTTATGATGTGGCTATTGAATCGCCCTTGCAGCTTGCGCCGACGTTGTCTGAACGGTTTGGGGTTAACATCTGGCTGAAGAGGGAGGACTTGCAGCCTGTGTTCTCATTCAAGCTCCGAGGAGCTTATAATATGATGGCCAAACTTCCAAAAGAACAGTTGGAAAAAGGAGTTATATGCTCTTCAGCTGGAAATCATGCTCAAGGGGTTGCATTAGCTGCTAAGCGATTAAGATGTAATGCTGTTATTGCTATGCCAGTTACTACGCCTGAAATTAAGTGGCAATCAGTTCAAAAATTGGGTGCGACGGTTGTTCTTGTTGGAGATTCATACGATGAGGCACAAGCATATGCTAAAAAGCGGAGCGTAGAGGAAGGCCGCACATTCATTCCCCCTTTCGATCACCCTGATGTTATAGCAGGGCAGGGTACAGTTGGGATGGAAATTGTGCGTCAAATAAAAGGTCCAGTGCATGCAATTTTTGTACCAGTTGGGGGCGGTGGTCTAATAGCTGGTATTGCTGCTTATGTCAAAAGGGTTTCTCCTGAGGTAAAGATCATTGGGGTAGAGCCATCTGATGCAAATGCTATGGCATTATCATTATGTCATGGCCAACGAGTAATATTGGACAAGGCTGGAGGCTTTGCTGATGGTGTAGCAGTTAAAGAAGTCGGTGAAGAGACTTTTCGCCTTTGCAAAGATCTCATGGATGGTGTAGTTCTTGTTAGCCGTGATGCTATATGTGCCTCGATAAAGGATATGTTTGAGGAGAAGAGAAGCATCTTGGAACCTGCAGGTGCTCTTTCTCTTGCTGGAGCTGAGGCCTACTGCAAATATTATGGCTTGAAGGGAGAAAATGTTGTGGTAATAACCAGTGGTGCAAATATGAACTTTGACAAACTGAGTATAGTTACTGAACTTGCCAATGTTGGTCGTGAGAAAGAGGCTGTGCTTGCAACTATATTGCCGGAGACACCTGGAAGCTTTAAAAAATTTTGTGAACTGGTTGGGCCTATGAACATTACAGAATTCAAGTACAGATATAGTTCTGAAAAAGAAGCTGTTGTTCTTTATAGGTAAGTGCTTATCAAGCTATTGGTAAGGAAAATTTAGAAATAAAAAACAGTGATTTAATGAGAATATTAAAACAAAAGCAAGGGAAACGGAAAGAGGAGTT

Coding sequence (CDS)

ATGGATGCTCTTCTTCTCAACGCACCCACTTCACACCCAATTCCTCGTAAATCCTTCCTTCCATCCCCTCCTTCCTTCTTCAATGGCTCCTCCATTTCGATTGATGACAATCGAAACAGAGTTCGACTTACCAGGCCTCCAATCATCCTAGCCACGCTTTCAAAGCACGCCCTTGATATCCCTCCTAATTCTGTATCCAACACTTCGGTTCCGACTCCTGGAGATGCATTGGCGCCTACCCCTTTGCCTCCTCGCAAAAGGGTATCGGCGGATTCTCTGCAGTTCCCGCCTGGCTATCTTGGCGCTGTTCCGGACCGTTCCGGTTCTGATAATGGCGAGGATAGTGTTGACGCCATGGAGTATTTGACTAGAATATTGGGTTCGAAGGTTTATGATGTGGCTATTGAATCGCCCTTGCAGCTTGCGCCGACGTTGTCTGAACGGTTTGGGGTTAACATCTGGCTGAAGAGGGAGGACTTGCAGCCTGTGTTCTCATTCAAGCTCCGAGGAGCTTATAATATGATGGCCAAACTTCCAAAAGAACAGTTGGAAAAAGGAGTTATATGCTCTTCAGCTGGAAATCATGCTCAAGGGGTTGCATTAGCTGCTAAGCGATTAAGATGTAATGCTGTTATTGCTATGCCAGTTACTACGCCTGAAATTAAGTGGCAATCAGTTCAAAAATTGGGTGCGACGGTTGTTCTTGTTGGAGATTCATACGATGAGGCACAAGCATATGCTAAAAAGCGGAGCGTAGAGGAAGGCCGCACATTCATTCCCCCTTTCGATCACCCTGATGTTATAGCAGGGCAGGGTACAGTTGGGATGGAAATTGTGCGTCAAATAAAAGGTCCAGTGCATGCAATTTTTGTACCAGTTGGGGGCGGTGGTCTAATAGCTGGTATTGCTGCTTATGTCAAAAGGGTTTCTCCTGAGGTAAAGATCATTGGGGTAGAGCCATCTGATGCAAATGCTATGGCATTATCATTATGTCATGGCCAACGAGTAATATTGGACAAGGCTGGAGGCTTTGCTGATGGTGTAGCAGTTAAAGAAGTCGGTGAAGAGACTTTTCGCCTTTGCAAAGATCTCATGGATGGTGTAGTTCTTGTTAGCCGTGATGCTATATGTGCCTCGATAAAGGATATGTTTGAGGAGAAGAGAAGCATCTTGGAACCTGCAGGTGCTCTTTCTCTTGCTGGAGCTGAGGCCTACTGCAAATATTATGGCTTGAAGGGAGAAAATGTTGTGGTAATAACCAGTGGTGCAAATATGAACTTTGACAAACTGAGTATAGTTACTGAACTTGCCAATGTTGGTCGTGAGAAAGAGGCTGTGCTTGCAACTATATTGCCGGAGACACCTGGAAGCTTTAAAAAATTTTGTGAACTGGTTGGGCCTATGAACATTACAGAATTCAAGTACAGATATAGTTCTGAAAAAGAAGCTGTTGTTCTTTATAGGTAA

Protein sequence

MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDIPPNSVSNTSVPTPGDALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAMEYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSEKEAVVLYR*

Homology

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BLAST of CSPI06G25030.2 vs. ExPASy Swiss-Prot
Match: Q9ZSS6 (Threonine dehydratase biosynthetic, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OMR1 PE=1 SV=1)

HSP 1 Score: 657.1 bits (1694), Expect = 1.5e-187
Identity = 339/453 (74.83%), Postives = 382/453 (84.33%), Query Frame = 0


Query: 38  RNRVRLTRPPIILAT--LSKHALDIPPNSVSNTSVPTPGDALAPTPLP-PRKRVSADSLQ 97
           R+ +     P++  T   S+  + +   S   TS+  P     P  LP PR +VS +SLQ
Sbjct: 14  RSHIHRPSKPVVGFTHFSSRSRIAVAVLSRDETSMTPP-----PPKLPLPRLKVSPNSLQ 73

Query: 98  FPPGYLGAVPDRSGSDNGEDSVDAMEYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIW 157
           +P GYLGAVP+R+         +AMEYLT IL +KVYD+AIESPLQLA  LS+R GV ++
Sbjct: 74  YPAGYLGAVPERTNEAENGSIAEAMEYLTNILSTKVYDIAIESPLQLAKKLSKRLGVRMY 133

Query: 158 LKREDLQPVFSFKLRGAYNMMAKLPKEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAM 217
           LKREDLQPVFSFKLRGAYNMM KLP +QL KGVICSSAGNHAQGVAL+A +L C AVI M
Sbjct: 134 LKREDLQPVFSFKLRGAYNMMVKLPADQLAKGVICSSAGNHAQGVALSASKLGCTAVIVM 193

Query: 218 PVTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTV 277
           PVTTPEIKWQ+V+ LGATVVL GDSYD+AQA+AK R+ EEG TFIPPFDHPDVIAGQGTV
Sbjct: 194 PVTTPEIKWQAVENLGATVVLFGDSYDQAQAHAKIRAEEEGLTFIPPFDHPDVIAGQGTV 253

Query: 278 GMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQ 337
           GMEI RQ KGP+HAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEP+DANAMALSL HG+
Sbjct: 254 GMEITRQAKGPLHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPADANAMALSLHHGE 313

Query: 338 RVILDKAGGFADGVAVKEVGEETFRLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPA 397
           RVILD+ GGFADGVAVKEVGEETFR+ ++LMDGVVLV+RDAICASIKDMFEEKR+ILEPA
Sbjct: 314 RVILDQVGGFADGVAVKEVGEETFRISRNLMDGVVLVTRDAICASIKDMFEEKRNILEPA 373

Query: 398 GALSLAGAEAYCKYYGLKGENVVVITSGANMNFDKLSIVTELANVGREKEAVLATILPET 457
           GAL+LAGAEAYCKYYGLK  NVV ITSGANMNFDKL IVTELANVGR++EAVLAT++PE 
Sbjct: 374 GALALAGAEAYCKYYGLKDVNVVAITSGANMNFDKLRIVTELANVGRQQEAVLATLMPEK 433

Query: 458 PGSFKKFCELVGPMNITEFKYRYSSEKEAVVLY 488
           PGSFK+FCELVGPMNI+EFKYR SSEKEAVVLY
Sbjct: 434 PGSFKQFCELVGPMNISEFKYRCSSEKEAVVLY 461

[Close]

BLAST of CSPI06G25030.2 vs. ExPASy Swiss-Prot
Match: A0FKE6 (Threonine dehydratase 1 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4081 GN=TD1 PE=1 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 1.0e-183
Identity = 338/465 (72.69%), Postives = 383/465 (82.37%), Query Frame = 0


Query: 24  PSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDIPPNSVSNTSVPTPGDALAPTPLP 83
           P F   SS+ +  +  +V  TR     A +   A +I  +  + T       A AP PL 
Sbjct: 18  PEFTAMSSVIVPISTVKVSGTRKSKKKALICAKATEILSSPATVTEPLKAEPAEAPVPL- 77

Query: 84  PRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAMEYLTRILGSKVYDVAIESPLQLAP 143
              RVS  SLQ  PGYL  +P+      G   V   EYLT IL SKVYDVA E+PLQ AP
Sbjct: 78  --LRVSPSSLQCEPGYL--LPNSPVLGTG--GVTGYEYLTNILSSKVYDVAYETPLQKAP 137

Query: 144 TLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQLEKGVICSSAGNHAQGVALAA 203
            LSER GVN+WLKREDLQPVFSFK+RGAYNMMAKLPKEQLEKGVICSSAGNHAQGVAL+A
Sbjct: 138 KLSERLGVNVWLKREDLQPVFSFKIRGAYNMMAKLPKEQLEKGVICSSAGNHAQGVALSA 197

Query: 204 KRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSVEEGRTFIPPFD 263
           +RL C+AVI MPVTTP+IKW+SV++LGATVVLVGDSYDEAQAYAKKR+  EGRTFIPPFD
Sbjct: 198 QRLGCDAVIVMPVTTPDIKWKSVKRLGATVVLVGDSYDEAQAYAKKRAESEGRTFIPPFD 257

Query: 264 HPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDA 323
           HPDVI GQGTVGMEI RQ+K  +HAIFVPVGGGGLIAGIAAY+KRV+P++KIIGVEP DA
Sbjct: 258 HPDVIVGQGTVGMEINRQLKDNIHAIFVPVGGGGLIAGIAAYLKRVAPDIKIIGVEPLDA 317

Query: 324 NAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKDLMDGVVLVSRDAICASIKDM 383
           NA+ALSL HGQRV+LD+ GGFADGVAVK VGEET+RLC++L+DGVVLV RDAICASIKDM
Sbjct: 318 NALALSLHHGQRVMLDQVGGFADGVAVKVVGEETYRLCEELIDGVVLVGRDAICASIKDM 377

Query: 384 FEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGANMNFDKLSIVTELANVGREK 443
           FEEKRSILEPAGAL+LAGAEAYCKYYGLKGENVV ITSGANMNFD+L +VTELA+VGR++
Sbjct: 378 FEEKRSILEPAGALALAGAEAYCKYYGLKGENVVAITSGANMNFDRLRLVTELADVGRQR 437

Query: 444 EAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSEKE-AVVLY 488
           EAVLAT +PE PGSFKKF E+VGPMNITEFKYRY+S+KE A+VLY
Sbjct: 438 EAVLATFMPEDPGSFKKFAEMVGPMNITEFKYRYNSDKERALVLY 475

[Close]

BLAST of CSPI06G25030.2 vs. ExPASy Swiss-Prot
Match: Q9AXU4 (Threonine dehydratase OS=Nicotiana attenuata OX=49451 GN=TD PE=1 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 4.9e-146
Identity = 267/424 (62.97%), Postives = 330/424 (77.83%), Query Frame = 0


Query: 68  TSVPTPGD-ALAPTPLPPRKRVSADSLQFPPGYLGAVPDR--SGSDNGEDSVDAMEYLTR 127
           TS+P P    L  + L P    SADS+  P         +    + +G D+ +  +YL  
Sbjct: 52  TSIPKPSALPLKVSQLSP----SADSMPVPASLQDVEAGKLIENNPSGGDTEELFQYLVE 111

Query: 128 ILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQLE 187
           IL S+VYDVAI+SPLQ A  LS++ GVN W+KRED+Q VFSFKLRGAYNMM KL KEQLE
Sbjct: 112 ILASRVYDVAIDSPLQNAAKLSKKLGVNFWIKREDMQSVFSFKLRGAYNMMTKLSKEQLE 171

Query: 188 KGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEAQ 247
           +GVI +SAGNHAQGVAL A+RL+C A I MPVTTPEIK ++V+ L   VVL GD++D+AQ
Sbjct: 172 RGVITASAGNHAQGVALGAQRLKCTATIVMPVTTPEIKIEAVKNLDGKVVLHGDTFDKAQ 231

Query: 248 AYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIAA 307
            +A K + +EG TFIPPFDHPDVI GQGT+G EI RQ+K  +HA+FVPVGGGGLIAG+AA
Sbjct: 232 EHALKLAEDEGLTFIPPFDHPDVIIGQGTIGTEINRQLK-DIHAVFVPVGGGGLIAGVAA 291

Query: 308 YVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKDL 367
           Y KRV+P  KIIGVEP  A++M  SL HG+RV L++   FADGVAV  VGEETFRLCKDL
Sbjct: 292 YFKRVAPHTKIIGVEPFGASSMTQSLYHGERVKLEQVDNFADGVAVALVGEETFRLCKDL 351

Query: 368 MDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGAN 427
           +DG+VLVS DAI A++KD+++E R+ILE +GAL++AGAEAYCKYY +KGENVV I SGAN
Sbjct: 352 IDGMVLVSNDAISAAVKDVYDEGRNILETSGALAIAGAEAYCKYYNIKGENVVAIASGAN 411

Query: 428 MNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSS-EKEA 487
           M+F KL +V +LA++G ++EA+LAT +PE PGSFKKFCELVGPMNITEFKYRY+S  K+A
Sbjct: 412 MDFSKLKLVVDLADIGGQREALLATFMPEEPGSFKKFCELVGPMNITEFKYRYNSGRKQA 470

[Close]

BLAST of CSPI06G25030.2 vs. ExPASy Swiss-Prot
Match: P25306 (Threonine dehydratase 2 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4081 GN=TD2 PE=1 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 1.1e-121
Identity = 224/378 (59.26%), Postives = 284/378 (75.13%), Query Frame = 0


Query: 112 GEDSVDAMEYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGA 171
           G DS +  +YL  IL S VYDVAIESPL+LA  LS+R GVN ++KRED Q VFSFKLRGA
Sbjct: 88  GGDSDELFQYLVDILASPVYDVAIESPLELAEKLSDRLGVNFYIKREDKQRVFSFKLRGA 147

Query: 172 YNMMAKLPKEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGA 231
           YNMM+ L +E+L+KGVI +SAGNHAQGVALA +RL C A I MP TTP+IK  +V+ LG 
Sbjct: 148 YNMMSNLSREELDKGVITASAGNHAQGVALAGQRLNCVAKIVMPTTTPQIKIDAVRALGG 207

Query: 232 TVVLVGDSYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFV 291
            VVL G ++DEAQ +A + S ++G  +IPPFD P VI GQGT+G EI RQ+K  +HA+F+
Sbjct: 208 DVVLYGKTFDEAQTHALELSEKDGLKYIPPFDDPGVIKGQGTIGTEINRQLK-DIHAVFI 267

Query: 292 PVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVK 351
           PVGGGGLIAG+A + K+++P  KIIGVEP  A +M LSL  G RV L     FADGVAV 
Sbjct: 268 PVGGGGLIAGVATFFKQIAPNTKIIGVEPYGAASMTLSLHEGHRVKLSNVDTFADGVAVA 327

Query: 352 EVGEETFRLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGL 411
            VGE TF  C++L+DG+VLV+ D I A+IKD+++E R+ILE +GA+++AGA AYC++Y +
Sbjct: 328 LVGEYTFAKCQELIDGMVLVANDGISAAIKDVYDEGRNILETSGAVAIAGAAAYCEFYKI 387

Query: 412 KGENVVVITSGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNIT 471
           K EN+V I SGANM+F KL  VTELA +G  KEA+LAT + E  GSFK F  LVG +N T
Sbjct: 388 KNENIVAIASGANMDFSKLHKVTELAGLGSGKEALLATFMVEQQGSFKTFVGLVGSLNFT 447

Query: 472 EFKYRYSSE-KEAVVLYR 489
           E  YR++SE K A++LYR
Sbjct: 448 ELTYRFTSERKNALILYR 464

[Close]

BLAST of CSPI06G25030.2 vs. ExPASy Swiss-Prot
Match: P53607 (L-threonine dehydratase biosynthetic IlvA OS=Burkholderia multivorans (strain ATCC 17616 / 249) OX=395019 GN=ilvA PE=3 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 1.5e-115
Identity = 209/367 (56.95%), Postives = 271/367 (73.84%), Query Frame = 0


Query: 120 EYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLP 179
           +YL +IL ++VYDVA E+ L+ A  LS R    ++LKRED QPVFSFKLRGAYN MA +P
Sbjct: 5   DYLKKILTARVYDVAFETELEPARNLSARLRNPVYLKREDNQPVFSFKLRGAYNKMAHIP 64

Query: 180 KEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQK---LGATVVLV 239
            + L +GVI +SAGNHAQGVA +A R+   AVI +PVTTP++K  +V+     G  V+  
Sbjct: 65  ADALARGVITASAGNHAQGVAFSAARMGVKAVIVVPVTTPQVKVDAVRAHGGPGVEVIQA 124

Query: 240 GDSYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGG 299
           G+SY +A A+A K   E G TF+ PFD P VIAGQGT+ MEI+RQ +GP+HAIFVP+GGG
Sbjct: 125 GESYSDAYAHALKVQEERGLTFVHPFDDPYVIAGQGTIAMEILRQHQGPIHAIFVPIGGG 184

Query: 300 GLIAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEE 359
           GL AG+AAYVK V PE+K+IGV+  D+ AMA SL  G+RV L + G FADG AVK VGEE
Sbjct: 185 GLAAGVAAYVKAVRPEIKVIGVQAEDSCAMAQSLQAGKRVELAEVGLFADGTAVKLVGEE 244

Query: 360 TFRLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENV 419
           TFRLCK+ +DGVV V  DA+CA+IKD+F++ RS+LEP+GAL++AGA+ Y +  G++ + +
Sbjct: 245 TFRLCKEYLDGVVTVDTDALCAAIKDVFQDTRSVLEPSGALAVAGAKLYAEREGIENQTL 304

Query: 420 VVITSGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYR 479
           V +TSGANMNFD++  V E A VG  +EAV A  +PE  GSFK+FC LVG  N+TEF YR
Sbjct: 305 VAVTSGANMNFDRMRFVAERAEVGEAREAVFAVTIPEERGSFKRFCSLVGDRNVTEFNYR 364

Query: 480 YSSEKEA 484
            +  + A
Sbjct: 365 IADAQSA 371

[Close]

BLAST of CSPI06G25030.2 vs. ExPASy TrEMBL
Match: A0A0A0KII4 (Threonine dehydratase OS=Cucumis sativus OX=3659 GN=Csa_6G449240 PE=3 SV=1)

HSP 1 Score: 961.4 bits (2484), Expect = 1.4e-276
Identity = 486/487 (99.79%), Postives = 486/487 (99.79%), Query Frame = 0


Query: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDI 60
           MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDI
Sbjct: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDI 60

Query: 61  PPNSVSNTSVPTPGDALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120
           PPNSVSNTSVPTP DALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME
Sbjct: 61  PPNSVSNTSVPTPADALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120

Query: 121 YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK 180
           YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK
Sbjct: 121 YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK 180

Query: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240
           EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY
Sbjct: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240

Query: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300
           DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA
Sbjct: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300

Query: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360
           GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL
Sbjct: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360

Query: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420
           CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT
Sbjct: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420

Query: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480
           SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE
Sbjct: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480

Query: 481 KEAVVLY 488
           KEAVVLY
Sbjct: 481 KEAVVLY 487

[Close]

BLAST of CSPI06G25030.2 vs. ExPASy TrEMBL
Match: A0A5A7SGN7 (Threonine dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00800 PE=3 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 1.6e-272
Identity = 477/487 (97.95%), Postives = 483/487 (99.18%), Query Frame = 0


Query: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDI 60
           M+ALLLNAPTSHPIPRKSFLPSPPSFFNG+SISIDDNRNRVRLTRPP I ATLSKHA+DI
Sbjct: 1   MEALLLNAPTSHPIPRKSFLPSPPSFFNGTSISIDDNRNRVRLTRPPFIQATLSKHAVDI 60

Query: 61  PPNSVSNTSVPTPGDALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120
           PPNSVSNTS+PTPGDALAPTPLPPR+RVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME
Sbjct: 61  PPNSVSNTSIPTPGDALAPTPLPPRRRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120

Query: 121 YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK 180
           YLTRILGSKVYDVAIESPLQLAPTLSER G NIWLKREDLQPVFSFKLRGAYNMMAKLP+
Sbjct: 121 YLTRILGSKVYDVAIESPLQLAPTLSERLGNNIWLKREDLQPVFSFKLRGAYNMMAKLPQ 180

Query: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240
           EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY
Sbjct: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240

Query: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300
           DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA
Sbjct: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300

Query: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360
           GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL
Sbjct: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360

Query: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420
           CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT
Sbjct: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420

Query: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480
           SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE
Sbjct: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480

Query: 481 KEAVVLY 488
           KEAVVLY
Sbjct: 481 KEAVVLY 487

[Close]

BLAST of CSPI06G25030.2 vs. ExPASy TrEMBL
Match: A0A1S3CHM7 (Threonine dehydratase OS=Cucumis melo OX=3656 GN=LOC103500854 PE=3 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 1.6e-272
Identity = 477/487 (97.95%), Postives = 483/487 (99.18%), Query Frame = 0


Query: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDI 60
           M+ALLLNAPTSHPIPRKSFLPSPPSFFNG+SISIDDNRNRVRLTRPP I ATLSKHA+DI
Sbjct: 1   MEALLLNAPTSHPIPRKSFLPSPPSFFNGTSISIDDNRNRVRLTRPPFIQATLSKHAVDI 60

Query: 61  PPNSVSNTSVPTPGDALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120
           PPNSVSNTS+PTPGDALAPTPLPPR+RVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME
Sbjct: 61  PPNSVSNTSIPTPGDALAPTPLPPRRRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120

Query: 121 YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK 180
           YLTRILGSKVYDVAIESPLQLAPTLSER G NIWLKREDLQPVFSFKLRGAYNMMAKLP+
Sbjct: 121 YLTRILGSKVYDVAIESPLQLAPTLSERLGNNIWLKREDLQPVFSFKLRGAYNMMAKLPQ 180

Query: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240
           EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY
Sbjct: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240

Query: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300
           DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA
Sbjct: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300

Query: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360
           GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL
Sbjct: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360

Query: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420
           CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT
Sbjct: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420

Query: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480
           SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE
Sbjct: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480

Query: 481 KEAVVLY 488
           KEAVVLY
Sbjct: 481 KEAVVLY 487

[Close]

BLAST of CSPI06G25030.2 vs. ExPASy TrEMBL
Match: A0A6J1IPE0 (Threonine dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111477608 PE=3 SV=1)

HSP 1 Score: 843.2 bits (2177), Expect = 5.5e-241
Identity = 427/484 (88.22%), Postives = 450/484 (92.98%), Query Frame = 0


Query: 4   LLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDIPPN 63
           +LLNAPTSHP+ RK  L SP  F NG+SI IDDNRNR+R+TR P I ATLSKH ++  PN
Sbjct: 3   VLLNAPTSHPLSRKWSLLSPAFFLNGTSIPIDDNRNRIRVTRVPFIQATLSKHTVENLPN 62

Query: 64  SVSNTSVPTPGDALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAMEYLT 123
           SVSNT+V    DALA  PLPPRKRV ADSLQFPPGYLGAVPDR GSD+ EDS+DAMEYLT
Sbjct: 63  SVSNTAVVALEDALAAVPLPPRKRVLADSLQFPPGYLGAVPDRLGSDDEEDSLDAMEYLT 122

Query: 124 RILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL 183
           RILGSKVYDVAIESPLQLAP LSER GVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL
Sbjct: 123 RILGSKVYDVAIESPLQLAPMLSERLGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL 182

Query: 184 EKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA 243
           E+GVICSSAGNHAQGVALAA+RLRC+AVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA
Sbjct: 183 ERGVICSSAGNHAQGVALAARRLRCDAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA 242

Query: 244 QAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIA 303
           QAYAKKRS++EGRTFIPPFDHPDVIAGQGTVGMEIVRQ+K  +HAIFVPVGGGGLIAGIA
Sbjct: 243 QAYAKKRSIDEGRTFIPPFDHPDVIAGQGTVGMEIVRQMKSKLHAIFVPVGGGGLIAGIA 302

Query: 304 AYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKD 363
           AYVKRVSPEVKIIGVEPSDANAMALSLCHGQR+IL+KAGGFADGVAVKEVGEETFRLCK 
Sbjct: 303 AYVKRVSPEVKIIGVEPSDANAMALSLCHGQRIILEKAGGFADGVAVKEVGEETFRLCKG 362

Query: 364 LMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA 423
           LMDGVVLV RDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA
Sbjct: 363 LMDGVVLVGRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA 422

Query: 424 NMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSEKEA 483
           NMNFDKL IVTELANVGRE+EAVL T LPE PGSFK+FCEL+G MNITEFKYRY+SEKEA
Sbjct: 423 NMNFDKLRIVTELANVGREQEAVLVTKLPEKPGSFKRFCELIGAMNITEFKYRYNSEKEA 482

Query: 484 VVLY 488
           VVLY
Sbjct: 483 VVLY 486

[Close]

BLAST of CSPI06G25030.2 vs. ExPASy TrEMBL
Match: A0A6J1EDB3 (Threonine dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111433135 PE=3 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.7e-237
Identity = 422/484 (87.19%), Postives = 445/484 (91.94%), Query Frame = 0


Query: 4   LLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDIPPN 63
           +L NAPT HP+ RK  L SP    NG+SI IDDNRNR+R+ R P I ATLSKH ++  PN
Sbjct: 3   VLPNAPTPHPLSRKWSLLSPAFLLNGTSIPIDDNRNRIRVNRVPFIQATLSKHTVENLPN 62

Query: 64  SVSNTSVPTPGDALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAMEYLT 123
           SVSNT+V    DAL   PLPPRKRV ADSLQFPPGYLGAVPDR GSD+ EDS+DAMEYLT
Sbjct: 63  SVSNTAVVALEDALVAVPLPPRKRVLADSLQFPPGYLGAVPDRLGSDDEEDSLDAMEYLT 122

Query: 124 RILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL 183
           RILGSKVYDVAIESPLQLAP LSER GVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL
Sbjct: 123 RILGSKVYDVAIESPLQLAPMLSERLGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL 182

Query: 184 EKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA 243
           E+GVICSSAGNHAQGVALAA+RLRC+AVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA
Sbjct: 183 ERGVICSSAGNHAQGVALAARRLRCDAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA 242

Query: 244 QAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIA 303
           QAYAKKRSV+EGRTFIPPFDHPDVIAGQGTVGMEIVRQ+K  +HAIFVPVGGGGLIAGIA
Sbjct: 243 QAYAKKRSVDEGRTFIPPFDHPDVIAGQGTVGMEIVRQMKSKLHAIFVPVGGGGLIAGIA 302

Query: 304 AYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKD 363
           AYVKRVSPEVKIIGVEPSDANAMALSLCHGQR+IL+KAGGFADGVAVKEVGEETFRLCK 
Sbjct: 303 AYVKRVSPEVKIIGVEPSDANAMALSLCHGQRIILEKAGGFADGVAVKEVGEETFRLCKG 362

Query: 364 LMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA 423
           LMDGVVLV RDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA
Sbjct: 363 LMDGVVLVGRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA 422

Query: 424 NMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSEKEA 483
           NMNFDKL IVTELANVGRE+EAVL T LPE PGSFK+FCEL+G MNITEFKYRY+SE+EA
Sbjct: 423 NMNFDKLRIVTELANVGREQEAVLVTKLPEKPGSFKRFCELIGAMNITEFKYRYNSEEEA 482

Query: 484 VVLY 488
           VVLY
Sbjct: 483 VVLY 486

[Close]

BLAST of CSPI06G25030.2 vs. NCBI nr
Match: XP_004143291.1 (threonine dehydratase biosynthetic, chloroplastic [Cucumis sativus] >KGN48217.1 hypothetical protein Csa_003876 [Cucumis sativus])

HSP 1 Score: 961.4 bits (2484), Expect = 2.9e-276
Identity = 486/487 (99.79%), Postives = 486/487 (99.79%), Query Frame = 0


Query: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDI 60
           MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDI
Sbjct: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDI 60

Query: 61  PPNSVSNTSVPTPGDALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120
           PPNSVSNTSVPTP DALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME
Sbjct: 61  PPNSVSNTSVPTPADALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120

Query: 121 YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK 180
           YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK
Sbjct: 121 YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK 180

Query: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240
           EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY
Sbjct: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240

Query: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300
           DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA
Sbjct: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300

Query: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360
           GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL
Sbjct: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360

Query: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420
           CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT
Sbjct: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420

Query: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480
           SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE
Sbjct: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480

Query: 481 KEAVVLY 488
           KEAVVLY
Sbjct: 481 KEAVVLY 487

[Close]

BLAST of CSPI06G25030.2 vs. NCBI nr
Match: XP_008462516.1 (PREDICTED: threonine dehydratase biosynthetic, chloroplastic [Cucumis melo] >KAA0025308.1 threonine dehydratase biosynthetic [Cucumis melo var. makuwa] >TYK07361.1 threonine dehydratase biosynthetic [Cucumis melo var. makuwa])

HSP 1 Score: 948.0 bits (2449), Expect = 3.3e-272
Identity = 477/487 (97.95%), Postives = 483/487 (99.18%), Query Frame = 0


Query: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDI 60
           M+ALLLNAPTSHPIPRKSFLPSPPSFFNG+SISIDDNRNRVRLTRPP I ATLSKHA+DI
Sbjct: 1   MEALLLNAPTSHPIPRKSFLPSPPSFFNGTSISIDDNRNRVRLTRPPFIQATLSKHAVDI 60

Query: 61  PPNSVSNTSVPTPGDALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120
           PPNSVSNTS+PTPGDALAPTPLPPR+RVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME
Sbjct: 61  PPNSVSNTSIPTPGDALAPTPLPPRRRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAME 120

Query: 121 YLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPK 180
           YLTRILGSKVYDVAIESPLQLAPTLSER G NIWLKREDLQPVFSFKLRGAYNMMAKLP+
Sbjct: 121 YLTRILGSKVYDVAIESPLQLAPTLSERLGNNIWLKREDLQPVFSFKLRGAYNMMAKLPQ 180

Query: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240
           EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY
Sbjct: 181 EQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSY 240

Query: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300
           DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA
Sbjct: 241 DEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIA 300

Query: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360
           GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL
Sbjct: 301 GIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRL 360

Query: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420
           CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT
Sbjct: 361 CKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVIT 420

Query: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480
           SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE
Sbjct: 421 SGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSE 480

Query: 481 KEAVVLY 488
           KEAVVLY
Sbjct: 481 KEAVVLY 487

[Close]

BLAST of CSPI06G25030.2 vs. NCBI nr
Match: XP_038881616.1 (threonine dehydratase biosynthetic, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 895.6 bits (2313), Expect = 1.9e-256
Identity = 454/489 (92.84%), Postives = 473/489 (96.73%), Query Frame = 0


Query: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFN-GSSISIDDNRNRVRLTRPPIILATLSKHALD 60
           M+ALLLNAPTSHPI R+S L S PSF N G+SIS+DDNR+R+RLTRPP I ATLSKH +D
Sbjct: 1   MEALLLNAPTSHPIARRSLLLSSPSFLNGGASISLDDNRSRIRLTRPPFIQATLSKHTVD 60

Query: 61  IPPNSVSNTSVPTPGDALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGS-DNGEDSVDA 120
           IPPNSV+NTSVPT GDALA TPLPPRKRVSADSLQFPPGYLGAVPDRSGS D+GEDS++A
Sbjct: 61  IPPNSVTNTSVPTLGDALAATPLPPRKRVSADSLQFPPGYLGAVPDRSGSDDDGEDSLNA 120

Query: 121 MEYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKL 180
           MEYLTRILGSKVYDVAIESPLQLAPTLSER GVNIWLKREDLQPVFSFKLRGAYNMMAKL
Sbjct: 121 MEYLTRILGSKVYDVAIESPLQLAPTLSERLGVNIWLKREDLQPVFSFKLRGAYNMMAKL 180

Query: 181 PKEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGD 240
           PKEQL+KGVICSSAGNHAQGVALAAKRLRCNAVIAMP+TTP+IKWQSV+KLGATVVLVGD
Sbjct: 181 PKEQLDKGVICSSAGNHAQGVALAAKRLRCNAVIAMPLTTPQIKWQSVKKLGATVVLVGD 240

Query: 241 SYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGL 300
           SYDEAQAYAKKR VEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGP+HAIFVPVGGGGL
Sbjct: 241 SYDEAQAYAKKRGVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPLHAIFVPVGGGGL 300

Query: 301 IAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETF 360
           IAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHG+R+ILDKAGGFADGVAVKEVGEETF
Sbjct: 301 IAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGERIILDKAGGFADGVAVKEVGEETF 360

Query: 361 RLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVV 420
           RLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYY LKGENVVV
Sbjct: 361 RLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYDLKGENVVV 420

Query: 421 ITSGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYS 480
           ITSGANMNFDKLSIVTELANVGREKEAVLATILPE PGSFK+FCELVGPMNITEFKYRYS
Sbjct: 421 ITSGANMNFDKLSIVTELANVGREKEAVLATILPEIPGSFKRFCELVGPMNITEFKYRYS 480

Query: 481 SEKEAVVLY 488
           SEKEAVVLY
Sbjct: 481 SEKEAVVLY 489

[Close]

BLAST of CSPI06G25030.2 vs. NCBI nr
Match: XP_038881617.1 (threonine dehydratase biosynthetic, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 895.6 bits (2313), Expect = 1.9e-256
Identity = 454/489 (92.84%), Postives = 473/489 (96.73%), Query Frame = 0


Query: 1   MDALLLNAPTSHPIPRKSFLPSPPSFFN-GSSISIDDNRNRVRLTRPPIILATLSKHALD 60
           M+ALLLNAPTSHPI R+S L S PSF N G+SIS+DDNR+R+RLTRPP I ATLSKH +D
Sbjct: 1   MEALLLNAPTSHPIARRSLLLSSPSFLNGGASISLDDNRSRIRLTRPPFIQATLSKHTVD 60

Query: 61  IPPNSVSNTSVPTPGDALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGS-DNGEDSVDA 120
           IPPNSV+NTSVPT GDALA TPLPPRKRVSADSLQFPPGYLGAVPDRSGS D+GEDS++A
Sbjct: 61  IPPNSVTNTSVPTLGDALAATPLPPRKRVSADSLQFPPGYLGAVPDRSGSDDDGEDSLNA 120

Query: 121 MEYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKL 180
           MEYLTRILGSKVYDVAIESPLQLAPTLSER GVNIWLKREDLQPVFSFKLRGAYNMMAKL
Sbjct: 121 MEYLTRILGSKVYDVAIESPLQLAPTLSERLGVNIWLKREDLQPVFSFKLRGAYNMMAKL 180

Query: 181 PKEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGD 240
           PKEQL+KGVICSSAGNHAQGVALAAKRLRCNAVIAMP+TTP+IKWQSV+KLGATVVLVGD
Sbjct: 181 PKEQLDKGVICSSAGNHAQGVALAAKRLRCNAVIAMPLTTPQIKWQSVKKLGATVVLVGD 240

Query: 241 SYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGL 300
           SYDEAQAYAKKR VEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGP+HAIFVPVGGGGL
Sbjct: 241 SYDEAQAYAKKRGVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPLHAIFVPVGGGGL 300

Query: 301 IAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETF 360
           IAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHG+R+ILDKAGGFADGVAVKEVGEETF
Sbjct: 301 IAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGERIILDKAGGFADGVAVKEVGEETF 360

Query: 361 RLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVV 420
           RLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYY LKGENVVV
Sbjct: 361 RLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYDLKGENVVV 420

Query: 421 ITSGANMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYS 480
           ITSGANMNFDKLSIVTELANVGREKEAVLATILPE PGSFK+FCELVGPMNITEFKYRYS
Sbjct: 421 ITSGANMNFDKLSIVTELANVGREKEAVLATILPEIPGSFKRFCELVGPMNITEFKYRYS 480

Query: 481 SEKEAVVLY 488
           SEKEAVVLY
Sbjct: 481 SEKEAVVLY 489

[Close]

BLAST of CSPI06G25030.2 vs. NCBI nr
Match: XP_022977238.1 (threonine dehydratase biosynthetic, chloroplastic-like [Cucurbita maxima] >XP_022977239.1 threonine dehydratase biosynthetic, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 843.2 bits (2177), Expect = 1.1e-240
Identity = 427/484 (88.22%), Postives = 450/484 (92.98%), Query Frame = 0


Query: 4   LLLNAPTSHPIPRKSFLPSPPSFFNGSSISIDDNRNRVRLTRPPIILATLSKHALDIPPN 63
           +LLNAPTSHP+ RK  L SP  F NG+SI IDDNRNR+R+TR P I ATLSKH ++  PN
Sbjct: 3   VLLNAPTSHPLSRKWSLLSPAFFLNGTSIPIDDNRNRIRVTRVPFIQATLSKHTVENLPN 62

Query: 64  SVSNTSVPTPGDALAPTPLPPRKRVSADSLQFPPGYLGAVPDRSGSDNGEDSVDAMEYLT 123
           SVSNT+V    DALA  PLPPRKRV ADSLQFPPGYLGAVPDR GSD+ EDS+DAMEYLT
Sbjct: 63  SVSNTAVVALEDALAAVPLPPRKRVLADSLQFPPGYLGAVPDRLGSDDEEDSLDAMEYLT 122

Query: 124 RILGSKVYDVAIESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL 183
           RILGSKVYDVAIESPLQLAP LSER GVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL
Sbjct: 123 RILGSKVYDVAIESPLQLAPMLSERLGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL 182

Query: 184 EKGVICSSAGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA 243
           E+GVICSSAGNHAQGVALAA+RLRC+AVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA
Sbjct: 183 ERGVICSSAGNHAQGVALAARRLRCDAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEA 242

Query: 244 QAYAKKRSVEEGRTFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIA 303
           QAYAKKRS++EGRTFIPPFDHPDVIAGQGTVGMEIVRQ+K  +HAIFVPVGGGGLIAGIA
Sbjct: 243 QAYAKKRSIDEGRTFIPPFDHPDVIAGQGTVGMEIVRQMKSKLHAIFVPVGGGGLIAGIA 302

Query: 304 AYVKRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKD 363
           AYVKRVSPEVKIIGVEPSDANAMALSLCHGQR+IL+KAGGFADGVAVKEVGEETFRLCK 
Sbjct: 303 AYVKRVSPEVKIIGVEPSDANAMALSLCHGQRIILEKAGGFADGVAVKEVGEETFRLCKG 362

Query: 364 LMDGVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA 423
           LMDGVVLV RDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA
Sbjct: 363 LMDGVVLVGRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGA 422

Query: 424 NMNFDKLSIVTELANVGREKEAVLATILPETPGSFKKFCELVGPMNITEFKYRYSSEKEA 483
           NMNFDKL IVTELANVGRE+EAVL T LPE PGSFK+FCEL+G MNITEFKYRY+SEKEA
Sbjct: 423 NMNFDKLRIVTELANVGREQEAVLVTKLPEKPGSFKRFCELIGAMNITEFKYRYNSEKEA 482

Query: 484 VVLY 488
           VVLY
Sbjct: 483 VVLY 486

[Close]

BLAST of CSPI06G25030.2 vs. TAIR 10
Match: AT3G10050.1 (L-O-methylthreonine resistant 1 )

HSP 1 Score: 657.1 bits (1694), Expect = 1.1e-188
Identity = 339/453 (74.83%), Postives = 382/453 (84.33%), Query Frame = 0


Query: 38  RNRVRLTRPPIILAT--LSKHALDIPPNSVSNTSVPTPGDALAPTPLP-PRKRVSADSLQ 97
           R+ +     P++  T   S+  + +   S   TS+  P     P  LP PR +VS +SLQ
Sbjct: 14  RSHIHRPSKPVVGFTHFSSRSRIAVAVLSRDETSMTPP-----PPKLPLPRLKVSPNSLQ 73

Query: 98  FPPGYLGAVPDRSGSDNGEDSVDAMEYLTRILGSKVYDVAIESPLQLAPTLSERFGVNIW 157
           +P GYLGAVP+R+         +AMEYLT IL +KVYD+AIESPLQLA  LS+R GV ++
Sbjct: 74  YPAGYLGAVPERTNEAENGSIAEAMEYLTNILSTKVYDIAIESPLQLAKKLSKRLGVRMY 133

Query: 158 LKREDLQPVFSFKLRGAYNMMAKLPKEQLEKGVICSSAGNHAQGVALAAKRLRCNAVIAM 217
           LKREDLQPVFSFKLRGAYNMM KLP +QL KGVICSSAGNHAQGVAL+A +L C AVI M
Sbjct: 134 LKREDLQPVFSFKLRGAYNMMVKLPADQLAKGVICSSAGNHAQGVALSASKLGCTAVIVM 193

Query: 218 PVTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSVEEGRTFIPPFDHPDVIAGQGTV 277
           PVTTPEIKWQ+V+ LGATVVL GDSYD+AQA+AK R+ EEG TFIPPFDHPDVIAGQGTV
Sbjct: 194 PVTTPEIKWQAVENLGATVVLFGDSYDQAQAHAKIRAEEEGLTFIPPFDHPDVIAGQGTV 253

Query: 278 GMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDANAMALSLCHGQ 337
           GMEI RQ KGP+HAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEP+DANAMALSL HG+
Sbjct: 254 GMEITRQAKGPLHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPADANAMALSLHHGE 313

Query: 338 RVILDKAGGFADGVAVKEVGEETFRLCKDLMDGVVLVSRDAICASIKDMFEEKRSILEPA 397
           RVILD+ GGFADGVAVKEVGEETFR+ ++LMDGVVLV+RDAICASIKDMFEEKR+ILEPA
Sbjct: 314 RVILDQVGGFADGVAVKEVGEETFRISRNLMDGVVLVTRDAICASIKDMFEEKRNILEPA 373

Query: 398 GALSLAGAEAYCKYYGLKGENVVVITSGANMNFDKLSIVTELANVGREKEAVLATILPET 457
           GAL+LAGAEAYCKYYGLK  NVV ITSGANMNFDKL IVTELANVGR++EAVLAT++PE 
Sbjct: 374 GALALAGAEAYCKYYGLKDVNVVAITSGANMNFDKLRIVTELANVGRQQEAVLATLMPEK 433

Query: 458 PGSFKKFCELVGPMNITEFKYRYSSEKEAVVLY 488
           PGSFK+FCELVGPMNI+EFKYR SSEKEAVVLY
Sbjct: 434 PGSFKQFCELVGPMNISEFKYRCSSEKEAVVLY 461

[Close]

BLAST of CSPI06G25030.2 vs. TAIR 10
Match: AT4G11640.1 (serine racemase )

HSP 1 Score: 156.0 bits (393), Expect = 7.8e-38
Identity = 96/297 (32.32%), Postives = 161/297 (54.21%), Query Frame = 0


Query: 137 SPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQLEKGVICSSAGNHA 196
           +P+  + +L+   G +++ K E LQ   +FK RGA N +  L  EQ  KGV+  S+GNHA
Sbjct: 29  TPVLTSESLNSISGRSLFFKCECLQKGGAFKFRGACNAVLSLDAEQAAKGVVTHSSGNHA 88

Query: 197 QGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSVEEGR 256
             ++LAAK     A I +P   P+ K  +V + G  V+    +    +  A K   E G 
Sbjct: 89  AALSLAAKIQGIPAYIVVPKGAPKCKVDNVIRYGGKVIWSEATMSSREEIASKVLQETGS 148

Query: 257 TFIPPFDHPDVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKII 316
             I P++   +I+GQGT+ +E++ QI+  + AI VP+ GGGLI+G+A   K + P ++II
Sbjct: 149 VLIHPYNDGRIISGQGTIALELLEQIQ-EIDAIVVPISGGGLISGVALAAKSIKPSIRII 208

Query: 317 GVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKDLMDGVVLVSRDAI 376
             EP  A+  A S   G+ + L      ADG+    +G+ T+ + +DL+D VV +    I
Sbjct: 209 AAEPKGADDAAQSKVAGKIITLPVTNTIADGLRA-SLGDLTWPVVRDLVDDVVTLEECEI 268

Query: 377 CASIKDMFEEKRSILEPAGALSLAG--AEAYCKYYGLKG-ENVVVITSGANMNFDKL 431
             ++K  +E  +  +EP+GA+ LA   + ++      +  +N+ ++ SG N++   L
Sbjct: 269 IEAMKMCYEILKVSVEPSGAIGLAAVLSNSFRNNPSCRDCKNIGIVLSGGNVDLGSL 323

[Close]

BLAST of CSPI06G25030.2 vs. TAIR 10
Match: AT3G61440.1 (cysteine synthase C1 )

HSP 1 Score: 65.1 bits (157), Expect = 1.8e-10
Identity = 74/320 (23.12%), Postives = 131/320 (40.94%), Query Frame = 0


Query: 136 ESPLQLAPTLSERFGVNIWLKREDLQPVFSFKLRGAYNMMAKLPKEQL----EKGVICSS 195
           ++PL     ++E     +  K+E  QP  S K R A  M+A   K++L    +  +I  +
Sbjct: 59  KTPLVFLNKVTEGCEAYVAAKQEHFQPTCSIKDRPAIAMIADAEKKKLIIPGKTTLIEPT 118

Query: 196 AGNHAQGVALAAKRLRCNAVIAMPVTTPEIKWQSVQKLGATVVLV----GDSYDEAQAYA 255
           +GN    +A  A       ++ MP  T   +  +++  GA +VL     G      +AY 
Sbjct: 119 SGNMGISLAFMAAMKGYRIIMTMPSYTSLERRVTMRSFGAELVLTDPAKGMGGTVKKAYD 178

Query: 256 KKRSVEEGRTFIPPFDHP-DVIAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYV 315
              S  +       F +P +      T G EI     G V    + +G GG ++G+  Y+
Sbjct: 179 LLDSTPDA-FMCQQFANPANTQIHFDTTGPEIWEDTLGNVDIFVMGIGSGGTVSGVGRYL 238

Query: 316 KRVSPEVKIIGVEPSDANAMALSLCHGQRVILDKAGGFADGVAVKEVGEETFRLCKDLMD 375
           K  +P VKI GVEP+++N            IL+        +    VG +   L  D+M+
Sbjct: 239 KSKNPNVKIYGVEPAESN------------ILNGGKPGPHAITGNGVGFKPEILDMDVME 298

Query: 376 GVVLVSRDAICASIKDMFEEKRSILEPAGALSLAGAEAYCKYYGLKGENVVVITSGANMN 435
            V+ VS +      +++  ++  ++  +   +   A    K    KG+ +V I +     
Sbjct: 299 SVLEVSSEDAIKMARELALKEGLMVGISSGANTVAAIRLAKMPENKGKLIVTIHASFGER 358

Query: 436 FDKLSIVTELANVGREKEAV 447
           +    +  EL     E + V
Sbjct: 359 YLSSVLFDELRKEAEEMKPV 365

[Close]

BLAST of CSPI06G25030.2 vs. TAIR 10
Match: AT2G43750.1 (O-acetylserine (thiol) lyase B )

HSP 1 Score: 62.0 bits (149), Expect = 1.5e-09
Identity = 45/176 (25.57%), Postives = 81/176 (46.02%), Query Frame = 0


Query: 156 KREDLQPVFSFKLRGAYNMMAKLPKEQL----EKGVICSSAGNHAQGVALAAKRLRCNAV 215
           K E ++P  S K R  Y+M+    ++ L    +  ++ S++GN   G+A  A       +
Sbjct: 105 KLEIMEPCCSVKDRIGYSMITDAEEKGLITPGKSVLVESTSGNTGIGLAFIAASKGYKLI 164

Query: 216 IAMPVTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSV---EEGRTFIPPFDHP-DV 275
           + MP +    +   ++  GA +VL   +     A  K   +         +  FD+P + 
Sbjct: 165 LTMPASMSLERRVLLRAFGAELVLTEPAKGMTGAIQKAEEILKKTPNSYMLQQFDNPANP 224

Query: 276 IAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDA 324
                T G EI    +G +  +   +G GG I G+  ++K   PE+K+IGVEP+++
Sbjct: 225 KIHYETTGPEIWEDTRGKIDILVAGIGTGGTITGVGRFIKERKPELKVIGVEPTES 280

[Close]

BLAST of CSPI06G25030.2 vs. TAIR 10
Match: AT2G43750.2 (O-acetylserine (thiol) lyase B )

HSP 1 Score: 62.0 bits (149), Expect = 1.5e-09
Identity = 45/176 (25.57%), Postives = 81/176 (46.02%), Query Frame = 0


Query: 156 KREDLQPVFSFKLRGAYNMMAKLPKEQL----EKGVICSSAGNHAQGVALAAKRLRCNAV 215
           K E ++P  S K R  Y+M+    ++ L    +  ++ S++GN   G+A  A       +
Sbjct: 105 KLEIMEPCCSVKDRIGYSMITDAEEKGLITPGKSVLVESTSGNTGIGLAFIAASKGYKLI 164

Query: 216 IAMPVTTPEIKWQSVQKLGATVVLVGDSYDEAQAYAKKRSV---EEGRTFIPPFDHP-DV 275
           + MP +    +   ++  GA +VL   +     A  K   +         +  FD+P + 
Sbjct: 165 LTMPASMSLERRVLLRAFGAELVLTEPAKGMTGAIQKAEEILKKTPNSYMLQQFDNPANP 224

Query: 276 IAGQGTVGMEIVRQIKGPVHAIFVPVGGGGLIAGIAAYVKRVSPEVKIIGVEPSDA 324
                T G EI    +G +  +   +G GG I G+  ++K   PE+K+IGVEP+++
Sbjct: 225 KIHYETTGPEIWEDTRGKIDILVAGIGTGGTITGVGRFIKERKPELKVIGVEPTES 280

The following BLAST results are available for this feature:

BLAST of CSPI06G25030.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZSS6	1.5e-187	74.83	Threonine dehydratase biosynthetic, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
A0FKE6	1.0e-183	72.69	Threonine dehydratase 1 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4...	[more]
Q9AXU4	4.9e-146	62.97	Threonine dehydratase OS=Nicotiana attenuata OX=49451 GN=TD PE=1 SV=1	[more]
P25306	1.1e-121	59.26	Threonine dehydratase 2 biosynthetic, chloroplastic OS=Solanum lycopersicum OX=4...	[more]
P53607	1.5e-115	56.95	L-threonine dehydratase biosynthetic IlvA OS=Burkholderia multivorans (strain AT...	[more]

BLAST of CSPI06G25030.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KII4	1.4e-276	99.79	Threonine dehydratase OS=Cucumis sativus OX=3659 GN=Csa_6G449240 PE=3 SV=1	[more]
A0A5A7SGN7	1.6e-272	97.95	Threonine dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold20...	[more]
A0A1S3CHM7	1.6e-272	97.95	Threonine dehydratase OS=Cucumis melo OX=3656 GN=LOC103500854 PE=3 SV=1	[more]
A0A6J1IPE0	5.5e-241	88.22	Threonine dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111477608 PE=3 SV=1	[more]
A0A6J1EDB3	1.7e-237	87.19	Threonine dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111433135 PE=3 SV=1	[more]

BLAST of CSPI06G25030.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004143291.1	2.9e-276	99.79	threonine dehydratase biosynthetic, chloroplastic [Cucumis sativus] >KGN48217.1 ...	[more]
XP_008462516.1	3.3e-272	97.95	PREDICTED: threonine dehydratase biosynthetic, chloroplastic [Cucumis melo] >KAA...	[more]
XP_038881616.1	1.9e-256	92.84	threonine dehydratase biosynthetic, chloroplastic-like isoform X1 [Benincasa his...	[more]
XP_038881617.1	1.9e-256	92.84	threonine dehydratase biosynthetic, chloroplastic-like isoform X2 [Benincasa his...	[more]
XP_022977238.1	1.1e-240	88.22	threonine dehydratase biosynthetic, chloroplastic-like [Cucurbita maxima] >XP_02...	[more]

BLAST of CSPI06G25030.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10050.1	1.1e-188	74.83	L-O-methylthreonine resistant 1	[more]
AT4G11640.1	7.8e-38	32.32	serine racemase	[more]
AT3G61440.1	1.8e-10	23.13	cysteine synthase C1	[more]
AT2G43750.1	1.5e-09	25.57	O-acetylserine (thiol) lyase B	[more]
AT2G43750.2	1.5e-09	25.57	O-acetylserine (thiol) lyase B	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001721	Threonine dehydratase, ACT-like domain	PFAM	PF00585	Thr_dehydrat_C	coord: 435..488 e-value: 6.8E-14 score: 51.5
IPR001721	Threonine dehydratase, ACT-like domain	PROSITE	PS51672	ACT_LIKE	coord: 445..488 score: 15.030569
IPR038110	Threonine dehydratase, ACT-like domain superfamily	GENE3D	3.40.1020.10	Biosynthetic Threonine Deaminase; Domain 3	coord: 444..488 e-value: 2.5E-13 score: 52.0
IPR001926	Pyridoxal-phosphate dependent enzyme	PFAM	PF00291	PALP	coord: 133..422 e-value: 3.2E-82 score: 276.2
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 170..261 e-value: 4.7E-109 score: 366.0
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 138..416 e-value: 4.7E-109 score: 366.0
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	SUPERFAMILY	53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 120..472
IPR005787	Threonine dehydratase, biosynthetic	TIGRFAM	TIGR01124	TIGR01124	coord: 120..485 e-value: 5.2E-162 score: 537.9
None	No IPR available	PIRSR	PIRSR006278-2	PIRSR006278-2	coord: 150..320 e-value: 3.4E-5 score: 21.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 61..82
None	No IPR available	PANTHER	PTHR48078	THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED	coord: 63..487
None	No IPR available	PANTHER	PTHR48078:SF1	THREONINE DEHYDRATASE	coord: 63..487
None	No IPR available	CDD	cd01562	Thr-dehyd	coord: 122..426 e-value: 2.62833E-144 score: 413.806
IPR000634	Serine/threonine dehydratase, pyridoxal-phosphate-binding site	PROSITE	PS00165	DEHYDRATASE_SER_THR	coord: 158..171

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI06G25030	CSPI06G25030	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G25030.2.utr5p1	CSPI06G25030.2.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G25030.2.cds1	CSPI06G25030.2.cds1	CDS
CSPI06G25030.2.cds2	CSPI06G25030.2.cds2	CDS
CSPI06G25030.2.cds3	CSPI06G25030.2.cds3	CDS
CSPI06G25030.2.cds4	CSPI06G25030.2.cds4	CDS
CSPI06G25030.2.cds5	CSPI06G25030.2.cds5	CDS
CSPI06G25030.2.cds6	CSPI06G25030.2.cds6	CDS
CSPI06G25030.2.cds7	CSPI06G25030.2.cds7	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G25030.2.utr3p1	CSPI06G25030.2.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI06G25030.2	CSPI06G25030.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006520	cellular amino acid metabolic process
biological_process	GO:0009097	isoleucine biosynthetic process
biological_process	GO:0006567	threonine catabolic process
molecular_function	GO:0004794	L-threonine ammonia-lyase activity
molecular_function	GO:0030170	pyridoxal phosphate binding