CSPI06G24280.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI06G24280.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Conserved peptide upstream open reading frame 46
Location	Chr6: 21761996 .. 21762974 (+)
Sequence length	979
RNA-Seq Expression	CSPI06G24280.1
Synteny	CSPI06G24280.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATCACCTTCTTCTATCCCCAAACCCTAACCCTACACTCTCCATTCACTTCCATTTCCACTCTCACACCCTCTCCCATGGATCTCAAGTTCCACAGATCCCCCATCTTACACGACCCTGCATTCGCAAAGCGGGTCTTCTTCCGTGTCTTCTTATTCGCTTCCGCCATCTCCCTCATTCCCATCCTCCACATTCTCACTTCTTACGATTTCAAATCCTTCCATTTACCCAAATCCCCACCCTGTCACGCCTCCCCTCACTCCACCCCCGATCATCTCCCCCGTGGCTCCTACCTCTTTCAGGGCCACTTCCTTAACCCCGTTTGGGATTCTTTCGATTCTCTCCATTGCCAGCACACTGTGAATCTCACCATCTCCCTCATCAAACTTCTTGTCCACGAAAAGCATCTCTTCAACCACAGCGCTAGAGCTCTCTTCGTCGGAGGAAGTTCTTCCTCCGCCGCTTCGGTGCTCCACGATTTGGGATTTTCCAGGGCTGTTGGGGTTGATAAAGGTCGGTTTATATCGCTGAAACGGATGGAAGTTGGGTATAAACTTGATTATCCAAATAGTTCGTTTGATTTTGTTTTGTTCAAGGGGAAGTTGAAGGTCTCTGTTCCTGATTTGGTTGTGGGTGAACTTGAACGGATTCTCGACGGCGGTGGAATTGGGGCGGTGGTTACCGGAATTAGTAGTCCAATATCGATTGGGTTGGGGGGAAGAGTGAGGAAATTACTGAAATCCTCATGTGTTGTATATTCGGGGAATGTTGAGAAGTTGTATGTGAGTGTATTTAAGAAGAAATCGTTTGATGATGAGGTTCCAATTAATTGTTCGAGTTAATTAGGTAAGATTAACTTCAAGAAAATTAGATAAACCATGTGGTCTCATGAATGTGGGTTGTTTGTAGGACTTGATCTGTATATACACTTACAACTACAATTATTGATGAACACTACAATTTGTTGCATTTCTATCTAC

mRNA sequence

CATCACCTTCTTCTATCCCCAAACCCTAACCCTACACTCTCCATTCACTTCCATTTCCACTCTCACACCCTCTCCCATGGATCTCAAGTTCCACAGATCCCCCATCTTACACGACCCTGCATTCGCAAAGCGGGTCTTCTTCCGTGTCTTCTTATTCGCTTCCGCCATCTCCCTCATTCCCATCCTCCACATTCTCACTTCTTACGATTTCAAATCCTTCCATTTACCCAAATCCCCACCCTGTCACGCCTCCCCTCACTCCACCCCCGATCATCTCCCCCGTGGCTCCTACCTCTTTCAGGGCCACTTCCTTAACCCCGTTTGGGATTCTTTCGATTCTCTCCATTGCCAGCACACTGTGAATCTCACCATCTCCCTCATCAAACTTCTTGTCCACGAAAAGCATCTCTTCAACCACAGCGCTAGAGCTCTCTTCGTCGGAGGAAGTTCTTCCTCCGCCGCTTCGGTGCTCCACGATTTGGGATTTTCCAGGGCTGTTGGGGTTGATAAAGGTCGGTTTATATCGCTGAAACGGATGGAAGTTGGGTATAAACTTGATTATCCAAATAGTTCGTTTGATTTTGTTTTGTTCAAGGGGAAGTTGAAGGTCTCTGTTCCTGATTTGGTTGTGGGTGAACTTGAACGGATTCTCGACGGCGGTGGAATTGGGGCGGTGGTTACCGGAATTAGTAGTCCAATATCGATTGGGTTGGGGGGAAGAGTGAGGAAATTACTGAAATCCTCATGTGTTGTATATTCGGGGAATGTTGAGAAGTTGTATGTGAGTGTATTTAAGAAGAAATCGTTTGATGATGAGGTTCCAATTAATTGTTCGAGTTAATTAGGTAAGATTAACTTCAAGAAAATTAGATAAACCATGTGGTCTCATGAATGTGGGTTGTTTGTAGGACTTGATCTGTATATACACTTACAACTACAATTATTGATGAACACTACAATTTGTTGCATTTCTATCTAC

Coding sequence (CDS)

ATGGATCTCAAGTTCCACAGATCCCCCATCTTACACGACCCTGCATTCGCAAAGCGGGTCTTCTTCCGTGTCTTCTTATTCGCTTCCGCCATCTCCCTCATTCCCATCCTCCACATTCTCACTTCTTACGATTTCAAATCCTTCCATTTACCCAAATCCCCACCCTGTCACGCCTCCCCTCACTCCACCCCCGATCATCTCCCCCGTGGCTCCTACCTCTTTCAGGGCCACTTCCTTAACCCCGTTTGGGATTCTTTCGATTCTCTCCATTGCCAGCACACTGTGAATCTCACCATCTCCCTCATCAAACTTCTTGTCCACGAAAAGCATCTCTTCAACCACAGCGCTAGAGCTCTCTTCGTCGGAGGAAGTTCTTCCTCCGCCGCTTCGGTGCTCCACGATTTGGGATTTTCCAGGGCTGTTGGGGTTGATAAAGGTCGGTTTATATCGCTGAAACGGATGGAAGTTGGGTATAAACTTGATTATCCAAATAGTTCGTTTGATTTTGTTTTGTTCAAGGGGAAGTTGAAGGTCTCTGTTCCTGATTTGGTTGTGGGTGAACTTGAACGGATTCTCGACGGCGGTGGAATTGGGGCGGTGGTTACCGGAATTAGTAGTCCAATATCGATTGGGTTGGGGGGAAGAGTGAGGAAATTACTGAAATCCTCATGTGTTGTATATTCGGGGAATGTTGAGAAGTTGTATGTGAGTGTATTTAAGAAGAAATCGTTTGATGATGAGGTTCCAATTAATTGTTCGAGTTAA

Protein sequence

MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASPHSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALFVGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGKLKVSVPDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVFKKKSFDDEVPINCSS*

Homology

[Close]

BLAST of CSPI06G24280.1 vs. ExPASy TrEMBL
Match: A0A0A0KEM7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G445050 PE=4 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 9.8e-141
Identity = 253/254 (99.61%), Postives = 254/254 (100.00%), Query Frame = 0


Query: 1   MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60
           MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP
Sbjct: 1   MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60

Query: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF 120
           HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF
Sbjct: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF 120

Query: 121 VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGKLKVSV 180
           VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGKLKVSV
Sbjct: 121 VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGKLKVSV 180

Query: 181 PDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVFK 240
           PDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVFK
Sbjct: 181 PDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVFK 240

Query: 241 KKSFDDEVPINCSS 255
           KKSFDD+VPINCSS
Sbjct: 241 KKSFDDDVPINCSS 254

[Close]

BLAST of CSPI06G24280.1 vs. ExPASy TrEMBL
Match: A0A1S3BMI8 (uncharacterized protein LOC103491650 OS=Cucumis melo OX=3656 GN=LOC103491650 PE=4 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 4.0e-126
Identity = 235/256 (91.80%), Postives = 243/256 (94.92%), Query Frame = 0


Query: 1   MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60
           MDLKF +SPIL DPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP
Sbjct: 1   MDLKFLKSPILRDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60

Query: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF 120
           HSTPDHLPRGSYLFQGHFLNPVWDSF+SLHC+ TVNLTISLIKLLV  KHLFNHSARALF
Sbjct: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFESLHCRETVNLTISLIKLLVDHKHLFNHSARALF 120

Query: 121 VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGK-LKVS 180
           VGGSSSSAASVL DLGFS A+GVDKGRFISLKRMEVGYKLDY N+SFDFVLF GK LKVS
Sbjct: 121 VGGSSSSAASVLQDLGFSDALGVDKGRFISLKRMEVGYKLDYANNSFDFVLFTGKLLKVS 180

Query: 181 VPDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVF 240
           VPDLVVGE+ERILDGGGIGAVVTGISSPISIG GGRVRKLLKSSCVVYSGNVEK+YVSVF
Sbjct: 181 VPDLVVGEIERILDGGGIGAVVTGISSPISIGFGGRVRKLLKSSCVVYSGNVEKMYVSVF 240

Query: 241 KKKSFDDEV-PINCSS 255
           KKKSF D+V PINCSS
Sbjct: 241 KKKSFHDQVIPINCSS 256

[Close]

BLAST of CSPI06G24280.1 vs. ExPASy TrEMBL
Match: A0A5A7U1B0 (Methyltransferase type 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold908G00620 PE=4 SV=1)

HSP 1 Score: 449.9 bits (1156), Expect = 7.1e-123
Identity = 228/248 (91.94%), Postives = 236/248 (95.16%), Query Frame = 0


Query: 1   MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60
           MDLKF +SPIL DPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP
Sbjct: 1   MDLKFLKSPILRDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60

Query: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF 120
           HSTPDHLPRGSYLFQGHFLNPVWDSF+SLHC+ TVNLTISLIKLLV  KHLFNHSARALF
Sbjct: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFESLHCRETVNLTISLIKLLVDHKHLFNHSARALF 120

Query: 121 VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGK-LKVS 180
           VGGSSSSAASVL DLGFS A+GVDKGRFISLKRMEVGYKLDY N+SFDFVLF GK LKVS
Sbjct: 121 VGGSSSSAASVLQDLGFSDALGVDKGRFISLKRMEVGYKLDYANNSFDFVLFTGKLLKVS 180

Query: 181 VPDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVF 240
           VPDLVVGE+ERILDGGGIGAVVTGISSPISIG GGRVRKLLKSSCVVYSGNVEK+YVSVF
Sbjct: 181 VPDLVVGEIERILDGGGIGAVVTGISSPISIGFGGRVRKLLKSSCVVYSGNVEKMYVSVF 240

Query: 241 KKKSFDDE 248
           KKKSF D+
Sbjct: 241 KKKSFHDQ 248

[Close]

BLAST of CSPI06G24280.1 vs. ExPASy TrEMBL
Match: A0A6J1EDK4 (uncharacterized protein LOC111433223 OS=Cucurbita moschata OX=3662 GN=LOC111433223 PE=4 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 1.1e-99
Identity = 185/254 (72.83%), Postives = 211/254 (83.07%), Query Frame = 0


Query: 1   MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60
           MDLK  +SPILHD AFA+R+ FR+FLFA A+S+IP +HI TSYDFKSFHLPKSPPCHA+ 
Sbjct: 1   MDLKLGKSPILHDAAFARRLCFRLFLFAFAVSIIPAVHIFTSYDFKSFHLPKSPPCHAAG 60

Query: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF 120
           H   D LPRGSYLFQGHFLNP+WDS +S HCQ TVNLTIS+I+ LV EKHLFNHSARALF
Sbjct: 61  HGGADQLPRGSYLFQGHFLNPIWDSLNSEHCQETVNLTISVIRELVDEKHLFNHSARALF 120

Query: 121 VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGKLKVSV 180
           VG SSS+AASVL DLGF  AVG+DKGRFIS+K+ EVGYKLDYPN SFDFVLF+GK K+SV
Sbjct: 121 VGESSSAAASVLQDLGFPGAVGIDKGRFISVKQREVGYKLDYPNDSFDFVLFRGKFKISV 180

Query: 181 PDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVFK 240
           PDLVVGE+ER+L GGG GAVV GI+SP++IG  GR+  LLKSSCVV S  V  L ++VFK
Sbjct: 181 PDLVVGEIERVLAGGGTGAVVIGINSPVTIGFAGRMPSLLKSSCVVSSKPVNNLLITVFK 240

Query: 241 KKSFDDEVPINCSS 255
           KK    E PINC S
Sbjct: 241 KKHL-HEFPINCPS 253

[Close]

BLAST of CSPI06G24280.1 vs. ExPASy TrEMBL
Match: A0A6J1IPF9 (uncharacterized protein LOC111477627 OS=Cucurbita maxima OX=3661 GN=LOC111477627 PE=4 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.7e-98
Identity = 183/254 (72.05%), Postives = 209/254 (82.28%), Query Frame = 0


Query: 1   MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60
           MDLK  +SPILHD AFA+R+ FR+FLFA A+S+IP +HI TSYD KSFHLPKSPPCH++ 
Sbjct: 1   MDLKLGKSPILHDAAFARRLCFRLFLFAFAVSIIPAVHIFTSYDSKSFHLPKSPPCHSAG 60

Query: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF 120
           H   D LPRGSYLFQGHFLNP+WDS +S HCQ TVNLTIS+I+ L  EKHLFNHSARALF
Sbjct: 61  HGGADQLPRGSYLFQGHFLNPIWDSLNSEHCQETVNLTISVIRELADEKHLFNHSARALF 120

Query: 121 VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGKLKVSV 180
           VGGSSS+AASVL DLGF  AVG+ KGRFISLK+ EVGYKLDYPN SFDFVLF+GK K+SV
Sbjct: 121 VGGSSSAAASVLRDLGFPGAVGIHKGRFISLKQREVGYKLDYPNDSFDFVLFRGKFKISV 180

Query: 181 PDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVFK 240
           PDLVVGE+ER+L GGG GAVV GISSP++IG  GR+  LLKSSCVV S  V  L ++VF+
Sbjct: 181 PDLVVGEIERVLAGGGTGAVVIGISSPVTIGFAGRMPSLLKSSCVVSSEPVNNLLITVFQ 240

Query: 241 KKSFDDEVPINCSS 255
           KK    E PINC S
Sbjct: 241 KKHL-HEFPINCPS 253

[Close]

BLAST of CSPI06G24280.1 vs. NCBI nr
Match: KAE8647389.1 (hypothetical protein Csa_003425 [Cucumis sativus])

HSP 1 Score: 509.2 bits (1310), Expect = 2.0e-140
Identity = 253/254 (99.61%), Postives = 254/254 (100.00%), Query Frame = 0


Query: 1   MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60
           MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP
Sbjct: 62  MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 121

Query: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF 120
           HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF
Sbjct: 122 HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF 181

Query: 121 VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGKLKVSV 180
           VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGKLKVSV
Sbjct: 182 VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGKLKVSV 241

Query: 181 PDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVFK 240
           PDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVFK
Sbjct: 242 PDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVFK 301

Query: 241 KKSFDDEVPINCSS 255
           KKSFDD+VPINCSS
Sbjct: 302 KKSFDDDVPINCSS 315

[Close]

BLAST of CSPI06G24280.1 vs. NCBI nr
Match: XP_011657647.1 (uncharacterized protein LOC105435880 [Cucumis sativus])

HSP 1 Score: 509.2 bits (1310), Expect = 2.0e-140
Identity = 253/254 (99.61%), Postives = 254/254 (100.00%), Query Frame = 0


Query: 1   MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60
           MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP
Sbjct: 1   MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60

Query: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF 120
           HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF
Sbjct: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF 120

Query: 121 VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGKLKVSV 180
           VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGKLKVSV
Sbjct: 121 VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGKLKVSV 180

Query: 181 PDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVFK 240
           PDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVFK
Sbjct: 181 PDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVFK 240

Query: 241 KKSFDDEVPINCSS 255
           KKSFDD+VPINCSS
Sbjct: 241 KKSFDDDVPINCSS 254

[Close]

BLAST of CSPI06G24280.1 vs. NCBI nr
Match: XP_008449921.1 (PREDICTED: uncharacterized protein LOC103491650 [Cucumis melo])

HSP 1 Score: 460.7 bits (1184), Expect = 8.3e-126
Identity = 235/256 (91.80%), Postives = 243/256 (94.92%), Query Frame = 0


Query: 1   MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60
           MDLKF +SPIL DPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP
Sbjct: 1   MDLKFLKSPILRDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60

Query: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF 120
           HSTPDHLPRGSYLFQGHFLNPVWDSF+SLHC+ TVNLTISLIKLLV  KHLFNHSARALF
Sbjct: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFESLHCRETVNLTISLIKLLVDHKHLFNHSARALF 120

Query: 121 VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGK-LKVS 180
           VGGSSSSAASVL DLGFS A+GVDKGRFISLKRMEVGYKLDY N+SFDFVLF GK LKVS
Sbjct: 121 VGGSSSSAASVLQDLGFSDALGVDKGRFISLKRMEVGYKLDYANNSFDFVLFTGKLLKVS 180

Query: 181 VPDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVF 240
           VPDLVVGE+ERILDGGGIGAVVTGISSPISIG GGRVRKLLKSSCVVYSGNVEK+YVSVF
Sbjct: 181 VPDLVVGEIERILDGGGIGAVVTGISSPISIGFGGRVRKLLKSSCVVYSGNVEKMYVSVF 240

Query: 241 KKKSFDDEV-PINCSS 255
           KKKSF D+V PINCSS
Sbjct: 241 KKKSFHDQVIPINCSS 256

[Close]

BLAST of CSPI06G24280.1 vs. NCBI nr
Match: KAA0047269.1 (Methyltransferase type 11 [Cucumis melo var. makuwa])

HSP 1 Score: 449.9 bits (1156), Expect = 1.5e-122
Identity = 228/248 (91.94%), Postives = 236/248 (95.16%), Query Frame = 0


Query: 1   MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60
           MDLKF +SPIL DPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP
Sbjct: 1   MDLKFLKSPILRDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60

Query: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALF 120
           HSTPDHLPRGSYLFQGHFLNPVWDSF+SLHC+ TVNLTISLIKLLV  KHLFNHSARALF
Sbjct: 61  HSTPDHLPRGSYLFQGHFLNPVWDSFESLHCRETVNLTISLIKLLVDHKHLFNHSARALF 120

Query: 121 VGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGK-LKVS 180
           VGGSSSSAASVL DLGFS A+GVDKGRFISLKRMEVGYKLDY N+SFDFVLF GK LKVS
Sbjct: 121 VGGSSSSAASVLQDLGFSDALGVDKGRFISLKRMEVGYKLDYANNSFDFVLFTGKLLKVS 180

Query: 181 VPDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVF 240
           VPDLVVGE+ERILDGGGIGAVVTGISSPISIG GGRVRKLLKSSCVVYSGNVEK+YVSVF
Sbjct: 181 VPDLVVGEIERILDGGGIGAVVTGISSPISIGFGGRVRKLLKSSCVVYSGNVEKMYVSVF 240

Query: 241 KKKSFDDE 248
           KKKSF D+
Sbjct: 241 KKKSFHDQ 248

[Close]

BLAST of CSPI06G24280.1 vs. NCBI nr
Match: XP_038883488.1 (uncharacterized protein LOC120074441 [Benincasa hispida])

HSP 1 Score: 423.3 bits (1087), Expect = 1.5e-114
Identity = 215/255 (84.31%), Postives = 230/255 (90.20%), Query Frame = 0


Query: 1   MDLKFHRSPILHDPAFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASP 60
           MDLKF +SPILHD AFA+R+FFR+FLF S ISLIPILHILTSYDFKSFHLPKSPPCHA  
Sbjct: 1   MDLKFVKSPILHDRAFARRIFFRIFLFGSVISLIPILHILTSYDFKSFHLPKSPPCHAVA 60

Query: 61  HSTP-DHLPRGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARAL 120
            +   D LPRGSYLFQGHFLNPVWDSFDS+HCQ TVNLT+S+IKLLV+EKHLFNHSARAL
Sbjct: 61  RTAAGDQLPRGSYLFQGHFLNPVWDSFDSVHCQETVNLTVSIIKLLVNEKHLFNHSARAL 120

Query: 121 FVGGSSSSAASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGKLKVS 180
           FVGGSSSSAAS+L DLGFS AVGVDKGRFISL++  VGYKLDY N SFDFVLFKGKLKVS
Sbjct: 121 FVGGSSSSAASMLQDLGFSGAVGVDKGRFISLEKRVVGYKLDYSNDSFDFVLFKGKLKVS 180

Query: 181 VPDLVVGELERILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVF 240
           VPDLVVGE+ERIL GGGIGAVVTGISSPISIGL GRV KLLKSSCVVYSGNV KLY+SVF
Sbjct: 181 VPDLVVGEIERILAGGGIGAVVTGISSPISIGLAGRVGKLLKSSCVVYSGNVNKLYISVF 240

Query: 241 KKKSFDDEVPINCSS 255
           KKK F  E+PINCSS
Sbjct: 241 KKKQF-HELPINCSS 254

[Close]

BLAST of CSPI06G24280.1 vs. TAIR 10
Match: AT3G53400.1 (BEST Arabidopsis thaliana protein match is: conserved peptide upstream open reading frame 47 (TAIR:AT5G03190.1); Has 285 Blast hits to 285 proteins in 23 species: Archae - 0; Bacteria - 0; Metazoa - 1; Fungi - 0; Plants - 279; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink). )

HSP 1 Score: 83.6 bits (205), Expect = 2.6e-16
Identity = 70/258 (27.13%), Postives = 116/258 (44.96%), Query Frame = 0


Query: 15  AFAKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASPHST------PDHLP 74
           +F +RV  R  +   A S++ +L  L    ++   +  + PC     +       P    
Sbjct: 14  SFRRRVILRALVIVCAFSVVSVLRNLAG-AYEGETINNTQPCKVDECAVNFAFLGPFLFS 73

Query: 75  RGSYLFQGHFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALFVGGSSSSA 134
               LF   FL PVW+  +S  C+  + LT  +++ L    +L ++ ++AL +G  S SA
Sbjct: 74  GNGLLFSNRFLKPVWNYLESDKCKDNIYLTTQVVRELT-GMNLLSNDSKALCIGRRSVSA 133

Query: 135 ASVLHDLGFSRAVGVDKGRFISLKRMEVGYKLDYPNSSFDFVLFKGKLKVSVPDLVVGEL 194
              ++  G S           + K  +   +L Y ++SF FV       V+VP  +V E+
Sbjct: 134 VLAMNRQGISDVSVAYMPPVFAFKHRKFTSELHYEDASFGFVFSMDLESVAVPASLVYEI 193

Query: 195 ERILDGGGIGAVVTGISSPIS----IGLGGRVRKLLKSSCVVYSGNVEKLYVSVFKKKSF 254
           ERIL  GG GA++ G +S       +     V  LLK+S VV+  ++ K  + VFK+   
Sbjct: 194 ERILKPGGTGAMLVGTTSGSDSNELVRSVSPVSSLLKNSSVVHVASLGKQVLVVFKRDGE 253

[Close]

BLAST of CSPI06G24280.1 vs. TAIR 10
Match: AT5G03190.2 (conserved peptide upstream open reading frame 47 )

HSP 1 Score: 67.4 bits (163), Expect = 1.9e-11
Identity = 70/252 (27.78%), Postives = 109/252 (43.25%), Query Frame = 0


Query: 17  AKRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASPHSTPDHLPRGSYLFQG 76
           ++   FR  + ASA+S++P+L +         H+          H   D +  G ++  G
Sbjct: 40  SRHALFRALIIASALSVVPLLELTAKN-----HV-------FGDHGAVDLVDLGRFVIPG 99

Query: 77  ------HFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALFVGGSSSSAAS 136
                   + P W   ++      V     L+  L+  K L ++ A+ L +G  S SA S
Sbjct: 100 PKLFFHKLVQPFWGKIETEKYPQVV--IADLVDELMGLK-LLHYDAKILCIGQGSDSAVS 159

Query: 137 VLHDLGFSRAVGVDKGRFIS-LKRMEVGYKLDYPNSSFDFVLFKGKLKVSVPDLVVGELE 196
              ++GFS   GV K    S   R  V       + SFDFVL      V+ P L+V E+E
Sbjct: 160 GFKEMGFSVVQGVPKHPLFSFFSRKHVNELELSGDKSFDFVLCGDVDHVASPALLVLEME 219

Query: 197 RILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVFKKKSFD---- 255
           R+L  GG GAV+   ++     L   V   LK S +V   N++K  V VFK+   +    
Sbjct: 220 RVLKPGGTGAVLVSTNAN---RLVKSVTSGLKQSEIVRVNNLDKFTVIVFKRNVTETAYC 273

[Close]

BLAST of CSPI06G24280.1 vs. TAIR 10
Match: AT5G03190.1 (conserved peptide upstream open reading frame 47 )

HSP 1 Score: 66.6 bits (161), Expect = 3.2e-11
Identity = 70/251 (27.89%), Postives = 108/251 (43.03%), Query Frame = 0


Query: 18  KRVFFRVFLFASAISLIPILHILTSYDFKSFHLPKSPPCHASPHSTPDHLPRGSYLFQG- 77
           +   FR  + ASA+S++P+L +         H+          H   D +  G ++  G 
Sbjct: 17  RHALFRALIIASALSVVPLLELTAKN-----HV-------FGDHGAVDLVDLGRFVIPGP 76

Query: 78  -----HFLNPVWDSFDSLHCQHTVNLTISLIKLLVHEKHLFNHSARALFVGGSSSSAASV 137
                  + P W   ++      V     L+  L+  K L ++ A+ L +G  S SA S 
Sbjct: 77  KLFFHKLVQPFWGKIETEKYPQVV--IADLVDELMGLK-LLHYDAKILCIGQGSDSAVSG 136

Query: 138 LHDLGFSRAVGVDKGRFIS-LKRMEVGYKLDYPNSSFDFVLFKGKLKVSVPDLVVGELER 197
             ++GFS   GV K    S   R  V       + SFDFVL      V+ P L+V E+ER
Sbjct: 137 FKEMGFSVVQGVPKHPLFSFFSRKHVNELELSGDKSFDFVLCGDVDHVASPALLVLEMER 196

Query: 198 ILDGGGIGAVVTGISSPISIGLGGRVRKLLKSSCVVYSGNVEKLYVSVFKKKSFD----- 255
           +L  GG GAV+   ++     L   V   LK S +V   N++K  V VFK+   +     
Sbjct: 197 VLKPGGTGAVLVSTNAN---RLVKSVTSGLKQSEIVRVNNLDKFTVIVFKRNVTETAYCI 249

The following BLAST results are available for this feature:

BLAST of CSPI06G24280.1 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI06G24280.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KEM7	9.8e-141	99.61	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G445050 PE=4 SV=1	[more]
A0A1S3BMI8	4.0e-126	91.80	uncharacterized protein LOC103491650 OS=Cucumis melo OX=3656 GN=LOC103491650 PE=...	[more]
A0A5A7U1B0	7.1e-123	91.94	Methyltransferase type 11 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffo...	[more]
A0A6J1EDK4	1.1e-99	72.83	uncharacterized protein LOC111433223 OS=Cucurbita moschata OX=3662 GN=LOC1114332...	[more]
A0A6J1IPF9	2.7e-98	72.05	uncharacterized protein LOC111477627 OS=Cucurbita maxima OX=3661 GN=LOC111477627...	[more]

BLAST of CSPI06G24280.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8647389.1	2.0e-140	99.61	hypothetical protein Csa_003425 [Cucumis sativus]	[more]
XP_011657647.1	2.0e-140	99.61	uncharacterized protein LOC105435880 [Cucumis sativus]	[more]
XP_008449921.1	8.3e-126	91.80	PREDICTED: uncharacterized protein LOC103491650 [Cucumis melo]	[more]
KAA0047269.1	1.5e-122	91.94	Methyltransferase type 11 [Cucumis melo var. makuwa]	[more]
XP_038883488.1	1.5e-114	84.31	uncharacterized protein LOC120074441 [Benincasa hispida]	[more]

BLAST of CSPI06G24280.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53400.1	2.6e-16	27.13	BEST Arabidopsis thaliana protein match is: conserved peptide upstream open read...	[more]
AT5G03190.2	1.9e-11	27.78	conserved peptide upstream open reading frame 47	[more]
AT5G03190.1	3.2e-11	27.89	conserved peptide upstream open reading frame 47	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR47291	PEPTIDE UPSTREAM PROTEIN	coord: 1..245
None	No IPR available	PANTHER	PTHR47291:SF1	PEPTIDE UPSTREAM PROTEIN	coord: 1..245

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI06G24280	CSPI06G24280	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G24280.1.utr5p1	CSPI06G24280.1.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G24280.1.cds1	CSPI06G24280.1.cds1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G24280.1.utr3p1	CSPI06G24280.1.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI06G24280.1	CSPI06G24280.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008168	methyltransferase activity