CSPI06G23260.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI06G23260.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	DNA polymerase epsilon subunit 3
Location	Chr6: 20968654 .. 20971337 (-)
Sequence length	978
RNA-Seq Expression	CSPI06G23260.1
Synteny	CSPI06G23260.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGAACGATAATAATGGCGGGAAACAGATTCGAGGAGGCCGCCGGCACTCTTGACGCCGAAGAACCTTGAAGAAGAAGAAGACGGTGAGATATCGTTAAGGAAATAGAGAAAGATGGAGAAAAAAATAGGTGGCGGAAATGAAGAGCTTCCAAAGACTATAGTTCGTCGAGTGGTCAAAGAGAAGCTTTCTCAGTGCTCTCGTGACCAGGACATCTCCGTCAACAAAGATTCGCTTCTTGCGTTTTGCGAGAGCGCTCGAATCTTCATCCATTACCTCTCCGCTACGTAAGTTCCGCAATCTGCCTTAACATGTTTAGCTGTGAATGAAATTCTATCCATCCTGATGTTCGAAATTAGGGCTCGTTGTTTCTCTGATGTTTGCCTTTAAAACGAAAACGAAATAGATTCAAGCGATAAATCTATTGTTGTTATGGCGCTATGGTTAACGAAAATGTTGCTTTGTATTAGTTAATTCTTTTGTTTGTATTAACTGGTAGATTTCTAAGCAGGGCGAATGATATATGCAAGGAATCAAAGAGGCAGACGATTAAGGCGGAAGATGTATTGAAAGCTTTAGAAGATATGGAGTTTCCCGAATTGGTTAGGCCTCTCAAAGCCTCCCTCGACGGTGAGTAAGCTTCCTATCCCTGTGTTTAACCAATATATCCAGTTGCACTTTAGTTAAACACATGAATCTTATTTTCGATTAGGTTTTCTGCTTACCCTTTTTTCACTATTTGGTTTCCGAATGGTCTTTATGCATTTTCTCGATTTTCCTGCCTAAAAAAGCCTTGCATTGTTAGACGTTCAATCTCGACTTTAAACAAATTTCAGTAACACATCAGTAGAGTGGTTAAATAAATCCATATGCAAAGAGGATGCTGACATTATAAGACGAATCCTTATCATTTCAAATATACAAGATGAGTGAATTTGGCACCATGGCAAGGATAGGACAGGCGTTCAATGTTGGAAAAATCAAGTGACTTCACTTTCTCTATAAGATACATAAATTACTCTTCTCGTTGCCAATTATGTTTTCTTTTTTGTTTAGTTTTGCGGGAGGGGGTTTCCTCTGCCCTGCCCTTAGGCTGTTCTTTTGGTTCTTTTGATATTTATGTTTTTTTTAAATGAAAGTTCATAATATCTAAATCTAAAAGAGGCACCATCTCGAGGGACATCCTGGAACCTTTATTTTCTAATATTTAACAATGTAAAAGTGGATACATGGACACATTAGTATTTAATGCAAGTCTTAATGGCTGCCCTTAATCTTATATTACGCATGTAATATAAGAAGGTTGAATGAAATTCAACTCAGATTCCTTTCTTAGTTTTACTTCATTGAATTTTGATTCTGAGAGGAGCTGGCGAGCTCAAAGAACTAATGCATTCCTTTTATTAAAGGAGTATAGGATTTGATTAGATTGAATAAGCTCCTACAAAATAAATCTTCAAGTGTGTACAAATTCTTGGAAATTTTAGTTTGATGTCTTTTTATTTATCTGTATGTACAAATGCTATTGAGTATTGAGATATCGAGTTGCTTAAGCTTTGCTATGTTGCGATGAGTAAGAGATGGGATTTACTTTCTGGATTTCTTTTAGTACACACATAGTTAGAGCACTCCAAAGTGTCTGTAAAGTGAGCAAGAATATCTGTTTGCTAAGGTGTAAAACAAAACCTTGTTTATGGAATTCTGGAATAGTGGTATTTGACAACGTTTTTGTTTCGTAGTTGGATATTCGAGGATCGATATTGAGTCTGATCACACTTAACATTTTTGGATGGGGAGATGGATAAATTCTTGATGCTGCAATACAAGTTCCATGTGTAGATATTGTCTTACCCGTTGTTGTCATTGTTGCTTGCTTATTATTAGGCGAGGAATGAATTATAATGTCAAAGATAGTTTGTTTAAAATGTGAACGACTGCTGTACTGTGACTCGATTGTATGCAGACGACAATGCTAATCTCTGCCAGCTATGATTTTCAATCTCGTGTTTGCCAAATTCATGTTCACAATTATCCAATATTCGTTTTAAGATAAGCTAGCTGAACCTCTTTAATGATTTATTCATCATGCAACCTCTGTTTGGCTGAATACAGAATTCAGACGCAAGAATGCTGGAAAGAAGGCAGCTGCCTCCAGAGAAAAAGAAGCGAAAAAGAAGAGGAAGGTAGAAGAGCCAACAGTTGAAGGTACAGATGGTGAAAGTATAGAAGACGTTGGAAATGATGATAACGATGATAATGATGAAGACGAAGATGAAGATGAAAATAGAGCCAGTGATGACTGAGAAACATGATCTGTGTCTCTAGTTAGGTACTTGAAATGAGGTTACTCTACACTTGTACGTTATTTATTTTCAAATCAGAAAGAAACTATTTATGAGATTTTATACAAATCTTCTAGAAAAGGTTATCCTACACTTGCAGTTTGTATATTATGAACGGCAGCTTCACCTTTTAGCAAGTTCTATATCTCTTTGTTAGCATGGTTGGTTAAGAGAGTTTAAGGTTACGTCCACCCGTGAAAATGCAGTAGATCAATGGAGATAAAGAAAGTGGTCTCGTTTGATAACTTTTGGAGTTGTTTACATGTGTTGTGCAATTGTACTTAACACATGAAATTCATATTCTAATCTATAATGAAATAACGTGACAAAACCTATTTGTGAT

mRNA sequence

CAGAACGATAATAATGGCGGGAAACAGATTCGAGGAGGCCGCCGGCACTCTTGACGCCGAAGAACCTTGAAGAAGAAGAAGACGGTGAGATATCGTTAAGGAAATAGAGAAAGATGGAGAAAAAAATAGGTGGCGGAAATGAAGAGCTTCCAAAGACTATAGTTCGTCGAGTGGTCAAAGAGAAGCTTTCTCAGTGCTCTCGTGACCAGGACATCTCCGTCAACAAAGATTCGCTTCTTGCGTTTTGCGAGAGCGCTCGAATCTTCATCCATTACCTCTCCGCTACGGCGAATGATATATGCAAGGAATCAAAGAGGCAGACGATTAAGGCGGAAGATGTATTGAAAGCTTTAGAAGATATGGAGTTTCCCGAATTGGTTAGGCCTCTCAAAGCCTCCCTCGACGAATTCAGACGCAAGAATGCTGGAAAGAAGGCAGCTGCCTCCAGAGAAAAAGAAGCGAAAAAGAAGAGGAAGGTAGAAGAGCCAACAGTTGAAGGTACAGATGGTGAAAGTATAGAAGACGTTGGAAATGATGATAACGATGATAATGATGAAGACGAAGATGAAGATGAAAATAGAGCCAGTGATGACTGAGAAACATGATCTGTGTCTCTAGTTAGGTACTTGAAATGAGGTTACTCTACACTTGTACGTTATTTATTTTCAAATCAGAAAGAAACTATTTATGAGATTTTATACAAATCTTCTAGAAAAGGTTATCCTACACTTGCAGTTTGTATATTATGAACGGCAGCTTCACCTTTTAGCAAGTTCTATATCTCTTTGTTAGCATGGTTGGTTAAGAGAGTTTAAGGTTACGTCCACCCGTGAAAATGCAGTAGATCAATGGAGATAAAGAAAGTGGTCTCGTTTGATAACTTTTGGAGTTGTTTACATGTGTTGTGCAATTGTACTTAACACATGAAATTCATATTCTAATCTATAATGAAATAACGTGACAAAACCTATTTGTGAT

Coding sequence (CDS)

ATGGAGAAAAAAATAGGTGGCGGAAATGAAGAGCTTCCAAAGACTATAGTTCGTCGAGTGGTCAAAGAGAAGCTTTCTCAGTGCTCTCGTGACCAGGACATCTCCGTCAACAAAGATTCGCTTCTTGCGTTTTGCGAGAGCGCTCGAATCTTCATCCATTACCTCTCCGCTACGGCGAATGATATATGCAAGGAATCAAAGAGGCAGACGATTAAGGCGGAAGATGTATTGAAAGCTTTAGAAGATATGGAGTTTCCCGAATTGGTTAGGCCTCTCAAAGCCTCCCTCGACGAATTCAGACGCAAGAATGCTGGAAAGAAGGCAGCTGCCTCCAGAGAAAAAGAAGCGAAAAAGAAGAGGAAGGTAGAAGAGCCAACAGTTGAAGGTACAGATGGTGAAAGTATAGAAGACGTTGGAAATGATGATAACGATGATAATGATGAAGACGAAGATGAAGATGAAAATAGAGCCAGTGATGACTGA

Protein sequence

MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKRKVEEPTVEGTDGESIEDVGNDDNDDNDEDEDEDENRASDD*

Homology

[Close]

BLAST of CSPI06G23260.1 vs. ExPASy Swiss-Prot
Match: Q9JKP7 (DNA polymerase epsilon subunit 3 OS=Mus musculus OX=10090 GN=Pole3 PE=1 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 9.0e-12
Identity = 46/139 (33.09%), Postives = 84/139 (60.43%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ AN+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKRKVEEPTVEGTD 131
            A DVL A+E+MEF   + PLK +L+ +RR   GKK A+ ++K+ K K+  EE   +  +
Sbjct: 70  NASDVLSAMEEMEFQRFITPLKEALEAYRRDEKGKKEASEQKKKDKDKKDSEEQD-KSRE 129

Query: 132 GESIEDVGNDDNDDNDEDE 151
            E  ++   D++D N+E+E
Sbjct: 130 VEEEDEERLDEDDQNEEEE 142

[Close]

BLAST of CSPI06G23260.1 vs. ExPASy Swiss-Prot
Match: Q3SZN5 (DNA polymerase epsilon subunit 3 OS=Bos taurus OX=9913 GN=POLE3 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.0e-11
Identity = 48/149 (32.21%), Postives = 88/149 (59.06%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ AN+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKRKVEEPTVEGTD 131
            A DVL A+E+MEF   V PLK +L+ +RR+  GKK A+ ++K+ K K+         TD
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKK---------TD 129

Query: 132 GESIEDVGNDDNDDNDEDEDEDENRASDD 161
            E  +   ++DND+++E  +E+E    ++
Sbjct: 130 SEEQDKSRDEDNDEDEERLEEEEQNEEEE 144

[Close]

BLAST of CSPI06G23260.1 vs. ExPASy Swiss-Prot
Match: Q9NRF9 (DNA polymerase epsilon subunit 3 OS=Homo sapiens OX=9606 GN=POLE3 PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.0e-11
Identity = 48/149 (32.21%), Postives = 88/149 (59.06%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ AN+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKRKVEEPTVEGTD 131
            A DVL A+E+MEF   V PLK +L+ +RR+  GKK A+ ++K+ K K+         TD
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKK---------TD 129

Query: 132 GESIEDVGNDDNDDNDEDEDEDENRASDD 161
            E  +   ++DND+++E  +E+E    ++
Sbjct: 130 SEEQDKSRDEDNDEDEERLEEEEQNEEEE 144

[Close]

BLAST of CSPI06G23260.1 vs. ExPASy Swiss-Prot
Match: Q5R4W3 (DNA polymerase epsilon subunit 3 OS=Pongo abelii OX=9601 GN=POLE3 PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.0e-11
Identity = 48/149 (32.21%), Postives = 88/149 (59.06%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ AN+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKRKVEEPTVEGTD 131
            A DVL A+E+MEF   V PLK +L+ +RR+  GKK A+ ++K+ K K+         TD
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKK---------TD 129

Query: 132 GESIEDVGNDDNDDNDEDEDEDENRASDD 161
            E  +   ++DND+++E  +E+E    ++
Sbjct: 130 SEEQDKSRDEDNDEDEERLEEEEQNEEEE 144

[Close]

BLAST of CSPI06G23260.1 vs. ExPASy Swiss-Prot
Match: Q642A5 (DNA polymerase epsilon subunit 3 OS=Rattus norvegicus OX=10116 GN=Pole3 PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 7.6e-11
Identity = 47/149 (31.54%), Postives = 86/149 (57.72%), Query Frame = 0


Query: 12  LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDICKESKRQTI 71
           LP  ++ R++KE L        ++++K++  A   +A +F+ Y ++ AN+   + KR+T+
Sbjct: 10  LPNAVITRIIKEAL-----PDGVNISKEARSAISRAASVFVLYATSCANNFAMKGKRKTL 69

Query: 72  KAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKRKVEEPTVEGTD 131
            A DVL A+E+MEF   V PLK +L+ +RR+  GKK A+ ++K+ K K+  EE       
Sbjct: 70  NASDVLSAMEEMEFQRFVTPLKEALEAYRREQKGKKEASEQKKKDKDKKDCEE------- 129

Query: 132 GESIEDVGNDDNDDNDEDEDEDENRASDD 161
               +D   ++ D+++E  DE+E    ++
Sbjct: 130 ----QDKSREEEDEDEERLDEEEQNEEEE 142

[Close]

BLAST of CSPI06G23260.1 vs. ExPASy TrEMBL
Match: A0A0A0KEV7 (CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G425700 PE=4 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 2.9e-74
Identity = 159/160 (99.38%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60
           MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN
Sbjct: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120

Query: 121 KVEEPTVEGTDGESIEDVGNDDNDDNDEDEDEDENRASDD 161
           KVEEPTVEGTDGESI+DVGNDDNDDNDEDEDEDENRASDD
Sbjct: 121 KVEEPTVEGTDGESIDDVGNDDNDDNDEDEDEDENRASDD 160

[Close]

BLAST of CSPI06G23260.1 vs. ExPASy TrEMBL
Match: A0A1S3BNA4 (DNA polymerase epsilon subunit D OS=Cucumis melo OX=3656 GN=LOC103491421 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.3e-63
Identity = 144/160 (90.00%), Postives = 148/160 (92.50%), Query Frame = 0


Query: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60
           MEKKI  G EELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN
Sbjct: 1   MEKKIVAGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASL+EFRRKNAGKKAAA REKE KKKR
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRRKNAGKKAAALREKEVKKKR 120

Query: 121 KVEEPTVEGTDGESIEDVGNDDNDDNDEDEDEDENRASDD 161
           KVEEPTVEGTDGESI+ VG+DDN     DEDEDEN ASD+
Sbjct: 121 KVEEPTVEGTDGESIDGVGDDDN-----DEDEDENEASDN 155

[Close]

BLAST of CSPI06G23260.1 vs. ExPASy TrEMBL
Match: A0A6J1K184 (DNA polymerase epsilon subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111490783 PE=4 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 4.6e-59
Identity = 135/159 (84.91%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60
           MEKK+G   EELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESAR+FIHYLSAT+N
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARVFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASL+EFR KNAGKKAAASR KEAKK++
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 KVEEPTVEGTDGESIEDVGN---DDNDDNDEDEDEDENR 157
             EEPTVEGTDGES +DV N   DD+DDND+DEDEDE++
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDDDDDNDKDEDEDEDK 159

[Close]

BLAST of CSPI06G23260.1 vs. ExPASy TrEMBL
Match: A0A6J1H495 (DNA polymerase epsilon subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111459853 PE=4 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 3.0e-58
Identity = 138/165 (83.64%), Postives = 146/165 (88.48%), Query Frame = 0


Query: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60
           MEKK+G   EELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESARIFIHYLSAT+N
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASL+EFR KNAGKKAAASR KEAKK++
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 KVEEPTVEGTDGESIEDV-----GNDDNDDNDEDEDEDENRASDD 161
             EEPTVEGTDGES +DV      NDD +D DEDEDE EN  SDD
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDNDDGNDKDEDEDEVENGGSDD 165

[Close]

BLAST of CSPI06G23260.1 vs. ExPASy TrEMBL
Match: A0A6J1DJA4 (DNA polymerase epsilon subunit 3 OS=Momordica charantia OX=3673 GN=LOC111020575 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 1.1e-57
Identity = 138/167 (82.63%), Postives = 148/167 (88.62%), Query Frame = 0


Query: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60
           MEKK+    EELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESARIFIHYLSAT+N
Sbjct: 1   MEKKVAAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKA+DVLKALEDMEFPELVRPLKASL+EFRRK+AGKKAAASREKEAKKKR
Sbjct: 61  DICKESKRQTIKADDVLKALEDMEFPELVRPLKASLNEFRRKSAGKKAAASREKEAKKKR 120

Query: 121 KVEEPTVEGTDGESIEDVGNDDND-------DNDEDEDEDENRASDD 161
           K+EEPTVE    ES +DVGNDDND       D  EDEDE+EN ASD+
Sbjct: 121 KIEEPTVE---DESKDDVGNDDNDNDHDEDEDEVEDEDEEENGASDE 164

[Close]

BLAST of CSPI06G23260.1 vs. NCBI nr
Match: XP_004140162.1 (DNA polymerase epsilon subunit D [Cucumis sativus] >XP_031742448.1 DNA polymerase epsilon subunit D [Cucumis sativus] >KGN48033.1 hypothetical protein Csa_004222 [Cucumis sativus])

HSP 1 Score: 287.7 bits (735), Expect = 6.1e-74
Identity = 159/160 (99.38%), Postives = 160/160 (100.00%), Query Frame = 0


Query: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60
           MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN
Sbjct: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120

Query: 121 KVEEPTVEGTDGESIEDVGNDDNDDNDEDEDEDENRASDD 161
           KVEEPTVEGTDGESI+DVGNDDNDDNDEDEDEDENRASDD
Sbjct: 121 KVEEPTVEGTDGESIDDVGNDDNDDNDEDEDEDENRASDD 160

[Close]

BLAST of CSPI06G23260.1 vs. NCBI nr
Match: XP_008449581.1 (PREDICTED: DNA polymerase epsilon subunit D [Cucumis melo])

HSP 1 Score: 251.5 bits (641), Expect = 4.8e-63
Identity = 144/160 (90.00%), Postives = 148/160 (92.50%), Query Frame = 0


Query: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60
           MEKKI  G EELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN
Sbjct: 1   MEKKIVAGIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASL+EFRRKNAGKKAAA REKE KKKR
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRRKNAGKKAAALREKEVKKKR 120

Query: 121 KVEEPTVEGTDGESIEDVGNDDNDDNDEDEDEDENRASDD 161
           KVEEPTVEGTDGESI+ VG+DDN     DEDEDEN ASD+
Sbjct: 121 KVEEPTVEGTDGESIDGVGDDDN-----DEDEDENEASDN 155

[Close]

BLAST of CSPI06G23260.1 vs. NCBI nr
Match: XP_038900596.1 (DNA polymerase epsilon subunit 3 [Benincasa hispida] >XP_038900597.1 DNA polymerase epsilon subunit 3 [Benincasa hispida])

HSP 1 Score: 251.5 bits (641), Expect = 4.8e-63
Identity = 143/160 (89.38%), Postives = 149/160 (93.12%), Query Frame = 0


Query: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60
           MEKKIG   EELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSAT+ 
Sbjct: 1   MEKKIGAAIEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATSY 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASL+EFRRKNAGKKAAASREKE KKKR
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRRKNAGKKAAASREKEVKKKR 120

Query: 121 KVEEPTVEGTDGESIEDVGNDDNDDNDEDEDEDENRASDD 161
           K+EEPTVEGTDGES++ VG   NDDNDED +EDEN ASDD
Sbjct: 121 KIEEPTVEGTDGESVDGVG---NDDNDEDGNEDENGASDD 157

[Close]

BLAST of CSPI06G23260.1 vs. NCBI nr
Match: XP_022995161.1 (DNA polymerase epsilon subunit 3 [Cucurbita maxima])

HSP 1 Score: 237.3 bits (604), Expect = 9.4e-59
Identity = 135/159 (84.91%), Postives = 147/159 (92.45%), Query Frame = 0


Query: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60
           MEKK+G   EELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESAR+FIHYLSAT+N
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARVFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASL+EFR KNAGKKAAASR KEAKK++
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 KVEEPTVEGTDGESIEDVGN---DDNDDNDEDEDEDENR 157
             EEPTVEGTDGES +DV N   DD+DDND+DEDEDE++
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDDDDDNDKDEDEDEDK 159

[Close]

BLAST of CSPI06G23260.1 vs. NCBI nr
Match: XP_023533668.1 (DNA polymerase epsilon subunit 3 [Cucurbita pepo subsp. pepo] >XP_023533669.1 DNA polymerase epsilon subunit 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 236.1 bits (601), Expect = 2.1e-58
Identity = 140/165 (84.85%), Postives = 149/165 (90.30%), Query Frame = 0


Query: 1   MEKKIGGGNEELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATAN 60
           MEKK+G   EELPKTIVRRVVKEKLSQCSR+QDISVNKDSLLAFCESARIFIHYLSAT+N
Sbjct: 1   MEKKVGAEIEELPKTIVRRVVKEKLSQCSRNQDISVNKDSLLAFCESARIFIHYLSATSN 60

Query: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKAAASREKEAKKKR 120
           DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASL+EFR KNAGKKAAASR KEAKK++
Sbjct: 61  DICKESKRQTIKAEDVLKALEDMEFPELVRPLKASLNEFRSKNAGKKAAASRIKEAKKRK 120

Query: 121 KVEEPTVEGTDGESIEDVGNDDNDD---NDEDEDED--ENRASDD 161
             EEPTVEGTDGES +DV ND++DD   NDEDEDED  EN  SDD
Sbjct: 121 IEEEPTVEGTDGESKDDVENDNDDDDNGNDEDEDEDEVENGGSDD 165

[Close]

BLAST of CSPI06G23260.1 vs. TAIR 10
Match: AT2G27470.1 (nuclear factor Y, subunit B11 )

HSP 1 Score: 137.9 bits (346), Expect = 7.2e-33
Identity = 87/172 (50.58%), Postives = 118/172 (68.60%), Query Frame = 0


Query: 10  EELPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDICKESKRQ 69
           +ELP  IVRRVVK+KLS+CS D D+S++K++LLAF ESARIFIHYLSATAND CK+++RQ
Sbjct: 9   DELPLAIVRRVVKKKLSECSPDYDVSIHKEALLAFSESARIFIHYLSATANDFCKDARRQ 68

Query: 70  TIKAEDVLKALEDMEFPELVRPLKASLDEFRRKNAGKKA-------------------AA 129
           T+KA+DV KALE+M+F E + PLK+SL++F++KNAGKKA                    A
Sbjct: 69  TMKADDVFKALEEMDFSEFLEPLKSSLEDFKKKNAGKKAGAAAASYPAGGAALKSSSGTA 128

Query: 130 SREKEAKKKRKVEEPTVEGTDGESI--EDVGNDDNDDNDEDEDEDENRASDD 161
           S+ KE KK+++ E  T +G     I  E   ND+  +ND  E+E+ N   D+
Sbjct: 129 SKPKETKKRKQEEPSTQKGARKSKIDEETKRNDEETENDNTEEENGNDEEDE 180

[Close]

BLAST of CSPI06G23260.1 vs. TAIR 10
Match: AT5G23090.2 (nuclear factor Y, subunit B13 )

HSP 1 Score: 49.3 bits (116), Expect = 3.4e-06
Identity = 29/98 (29.59%), Postives = 54/98 (55.10%), Query Frame = 0


Query: 5   IGGGNEE--LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDI 64
           +G   E+  LPK  + +++KE L       D+ V +D+     E    FI+ +S+ +ND+
Sbjct: 7   VGKSKEDASLPKATMTKIIKEML-----PPDVRVARDAQDLLIECCVEFINLVSSESNDV 66

Query: 65  CKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFR 101
           C +  ++TI  E VLKAL+ + F E +  + A+ ++ +
Sbjct: 67  CNKEDKRTIAPEHVLKALQVLGFGEYIEEVYAAYEQHK 99

[Close]

BLAST of CSPI06G23260.1 vs. TAIR 10
Match: AT5G23090.1 (nuclear factor Y, subunit B13 )

HSP 1 Score: 49.3 bits (116), Expect = 3.4e-06
Identity = 29/98 (29.59%), Postives = 54/98 (55.10%), Query Frame = 0


Query: 5   IGGGNEE--LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDI 64
           +G   E+  LPK  + +++KE L       D+ V +D+     E    FI+ +S+ +ND+
Sbjct: 7   VGKSKEDASLPKATMTKIIKEML-----PPDVRVARDAQDLLIECCVEFINLVSSESNDV 66

Query: 65  CKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFR 101
           C +  ++TI  E VLKAL+ + F E +  + A+ ++ +
Sbjct: 67  CNKEDKRTIAPEHVLKALQVLGFGEYIEEVYAAYEQHK 99

[Close]

BLAST of CSPI06G23260.1 vs. TAIR 10
Match: AT5G08190.1 (nuclear factor Y, subunit B12 )

HSP 1 Score: 48.5 bits (114), Expect = 5.8e-06
Identity = 29/98 (29.59%), Postives = 54/98 (55.10%), Query Frame = 0


Query: 5   IGGGNEE--LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDI 64
           +G   E+  LPK  + +++KE L       D+ V +D+     E    FI+ +S+ +N++
Sbjct: 7   VGKSKEDASLPKATMTKIIKEML-----PADVRVARDAQDLLIECCVEFINLISSESNEV 66

Query: 65  CKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFR 101
           C +  ++TI  E VLKAL+ + F E V  + A+ ++ +
Sbjct: 67  CNKEDKRTIAPEHVLKALQVLGFGEYVEEVYAAYEQHK 99

[Close]

BLAST of CSPI06G23260.1 vs. TAIR 10
Match: AT5G08190.2 (nuclear factor Y, subunit B12 )

HSP 1 Score: 48.5 bits (114), Expect = 5.8e-06
Identity = 29/98 (29.59%), Postives = 54/98 (55.10%), Query Frame = 0


Query: 5   IGGGNEE--LPKTIVRRVVKEKLSQCSRDQDISVNKDSLLAFCESARIFIHYLSATANDI 64
           +G   E+  LPK  + +++KE L       D+ V +D+     E    FI+ +S+ +N++
Sbjct: 7   VGKSKEDASLPKATMTKIIKEML-----PADVRVARDAQDLLIECCVEFINLISSESNEV 66

Query: 65  CKESKRQTIKAEDVLKALEDMEFPELVRPLKASLDEFR 101
           C +  ++TI  E VLKAL+ + F E V  + A+ ++ +
Sbjct: 67  CNKEDKRTIAPEHVLKALQVLGFGEYVEEVYAAYEQHK 99

The following BLAST results are available for this feature:

BLAST of CSPI06G23260.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9JKP7	9.0e-12	33.09	DNA polymerase epsilon subunit 3 OS=Mus musculus OX=10090 GN=Pole3 PE=1 SV=1	[more]
Q3SZN5	2.0e-11	32.21	DNA polymerase epsilon subunit 3 OS=Bos taurus OX=9913 GN=POLE3 PE=2 SV=1	[more]
Q9NRF9	2.0e-11	32.21	DNA polymerase epsilon subunit 3 OS=Homo sapiens OX=9606 GN=POLE3 PE=1 SV=1	[more]
Q5R4W3	2.0e-11	32.21	DNA polymerase epsilon subunit 3 OS=Pongo abelii OX=9601 GN=POLE3 PE=2 SV=1	[more]
Q642A5	7.6e-11	31.54	DNA polymerase epsilon subunit 3 OS=Rattus norvegicus OX=10116 GN=Pole3 PE=1 SV=...	[more]

BLAST of CSPI06G23260.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KEV7	2.9e-74	99.38	CBFD_NFYB_HMF domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G4257...	[more]
A0A1S3BNA4	2.3e-63	90.00	DNA polymerase epsilon subunit D OS=Cucumis melo OX=3656 GN=LOC103491421 PE=4 SV...	[more]
A0A6J1K184	4.6e-59	84.91	DNA polymerase epsilon subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111490783 PE=...	[more]
A0A6J1H495	3.0e-58	83.64	DNA polymerase epsilon subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111459853 P...	[more]
A0A6J1DJA4	1.1e-57	82.63	DNA polymerase epsilon subunit 3 OS=Momordica charantia OX=3673 GN=LOC111020575 ...	[more]

BLAST of CSPI06G23260.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140162.1	6.1e-74	99.38	DNA polymerase epsilon subunit D [Cucumis sativus] >XP_031742448.1 DNA polymeras...	[more]
XP_008449581.1	4.8e-63	90.00	PREDICTED: DNA polymerase epsilon subunit D [Cucumis melo]	[more]
XP_038900596.1	4.8e-63	89.38	DNA polymerase epsilon subunit 3 [Benincasa hispida] >XP_038900597.1 DNA polymer...	[more]
XP_022995161.1	9.4e-59	84.91	DNA polymerase epsilon subunit 3 [Cucurbita maxima]	[more]
XP_023533668.1	2.1e-58	84.85	DNA polymerase epsilon subunit 3 [Cucurbita pepo subsp. pepo] >XP_023533669.1 DN...	[more]

BLAST of CSPI06G23260.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G27470.1	7.2e-33	50.58	nuclear factor Y, subunit B11	[more]
AT5G23090.2	3.4e-06	29.59	nuclear factor Y, subunit B13	[more]
AT5G23090.1	3.4e-06	29.59	nuclear factor Y, subunit B13	[more]
AT5G08190.1	5.8e-06	29.59	nuclear factor Y, subunit B12	[more]
AT5G08190.2	5.8e-06	29.59	nuclear factor Y, subunit B12	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009072	Histone-fold	GENE3D	1.10.20.10	Histone, subunit A	coord: 7..157 e-value: 1.7E-34 score: 120.6
IPR009072	Histone-fold	SUPERFAMILY	47113	Histone-fold	coord: 12..119
IPR003958	Transcription factor CBF/NF-Y/archaeal histone domain	PFAM	PF00808	CBFD_NFYB_HMF	coord: 11..80 e-value: 1.4E-9 score: 38.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 100..128
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 100..160
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 129..160
None	No IPR available	PANTHER	PTHR46172	DNA POLYMERASE EPSILON SUBUNIT 3	coord: 4..154

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI06G23260	CSPI06G23260	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G23260.1.utr3p1	CSPI06G23260.1.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G23260.1.cds3	CSPI06G23260.1.cds3	CDS
CSPI06G23260.1.cds2	CSPI06G23260.1.cds2	CDS
CSPI06G23260.1.cds1	CSPI06G23260.1.cds1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI06G23260.1.utr5p1	CSPI06G23260.1.utr5p1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI06G23260.1	CSPI06G23260.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006974	cellular response to DNA damage stimulus
biological_process	GO:0031507	heterochromatin assembly
biological_process	GO:0006272	leading strand elongation
biological_process	GO:0042766	nucleosome mobilization
cellular_component	GO:0008623	CHRAC
cellular_component	GO:0008622	epsilon DNA polymerase complex
molecular_function	GO:0046982	protein heterodimerization activity
molecular_function	GO:0031490	chromatin DNA binding