CSPI05G01400.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI05G01400.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Cytochrome b561 domain-containing protein
Location	Chr5: 1920801 .. 1921327 (+)
Sequence length	390
RNA-Seq Expression	CSPI05G01400.1
Synteny	CSPI05G01400.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATTAGCATGTGGAATCTTTGGGGTTTGCATGGCTTTCAAGTTTCACCATGAAATCAAACTTTCAGACCTTCTTTCATTGCATTCTTGGATTGGTTTGATAGCCGTTTCCTTGTATGCTTTCCAGGTCATTTCTCCCCTTAAACATTACTTTACTATAAGATAAAATTAAAGCTAAGTGCTAACGCATCGCAATTATTCAAGATATTTGTACAACATTTTTGTAAGATACATAATAATTGAAGTGTGTATATTCTTATGCAGTGGATATTTGGTTTCTTTGTGTACTTCTTCCCTGGAGCAGAAGCAAGAAGAAGAGGAAACATGTTGCCATGGCATGTTTACTTTGGAGTATTCATATTTTTAATAGCAACATGCAATGCTGAATTAGGGTTACTTCAAAGGTTCAATACATTGGTGTTAGGTCATTCACAACAAGGCCTTATTGTCAACTTCACTGGCCTTCTTCTTCTTCTCTATGCTGCCACTGTTGTTCTCACTGTCATTCTCCCACCACTTTATTAA

mRNA sequence

ATGGCATTAGCATGTGGAATCTTTGGGGTTTGCATGGCTTTCAAGTTTCACCATGAAATCAAACTTTCAGACCTTCTTTCATTGCATTCTTGGATTGGTTTGATAGCCGTTTCCTTGTATGCTTTCCAGTGGATATTTGGTTTCTTTGTGTACTTCTTCCCTGGAGCAGAAGCAAGAAGAAGAGGAAACATGTTGCCATGGCATGTTTACTTTGGAGTATTCATATTTTTAATAGCAACATGCAATGCTGAATTAGGGTTACTTCAAAGGTTCAATACATTGGTGTTAGGTCATTCACAACAAGGCCTTATTGTCAACTTCACTGGCCTTCTTCTTCTTCTCTATGCTGCCACTGTTGTTCTCACTGTCATTCTCCCACCACTTTATTAA

Coding sequence (CDS)

ATGGCATTAGCATGTGGAATCTTTGGGGTTTGCATGGCTTTCAAGTTTCACCATGAAATCAAACTTTCAGACCTTCTTTCATTGCATTCTTGGATTGGTTTGATAGCCGTTTCCTTGTATGCTTTCCAGTGGATATTTGGTTTCTTTGTGTACTTCTTCCCTGGAGCAGAAGCAAGAAGAAGAGGAAACATGTTGCCATGGCATGTTTACTTTGGAGTATTCATATTTTTAATAGCAACATGCAATGCTGAATTAGGGTTACTTCAAAGGTTCAATACATTGGTGTTAGGTCATTCACAACAAGGCCTTATTGTCAACTTCACTGGCCTTCTTCTTCTTCTCTATGCTGCCACTGTTGTTCTCACTGTCATTCTCCCACCACTTTATTAA

Protein sequence

MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARRRGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVVLTVILPPLY*

Homology

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BLAST of CSPI05G01400.1 vs. ExPASy Swiss-Prot
Match: Q8L856 (Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB561A PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 2.1e-27
Identity = 56/126 (44.44%), Postives = 83/126 (65.87%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +ALA GIFG+C AFK H+E  + +L SLHSWIG+  +SLY FQW++ F V+FFPG     
Sbjct: 89  IALALGIFGICAAFKNHNESHIPNLYSLHSWIGIGVISLYGFQWVYSFIVFFFPGGSTNL 148

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLG----HSQQGLIVNFTGLLLLLYA 120
           +  +LPWH   G+F++++A  NA LG L++   L  G    +  +  ++NFT ++ +L+ 
Sbjct: 149 KSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLENGGLDKYGSEAFLINFTAIITILFG 208

Query: 121 ATVVLT 123
           A VVLT
Sbjct: 209 AFVVLT 214

[Close]

BLAST of CSPI05G01400.1 vs. ExPASy Swiss-Prot
Match: Q7XMK3 (Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0533500 PE=2 SV=2)

HSP 1 Score: 117.9 bits (294), Expect = 8.8e-26
Identity = 56/130 (43.08%), Postives = 83/130 (63.85%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +A+  G  G+  AFKFH+E  +++L SLHSW+G+  +SLY  QWIFGF  +F+PGA    
Sbjct: 92  IAIVLGAVGIYCAFKFHNESGIANLYSLHSWLGIGTISLYGIQWIFGFVAFFYPGAAPHV 151

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLG----HSQQGLIVNFTGLLLLLYA 120
           R   LPWHV FG+F++++    AELGLL++   L       +  +  +VNFTGL++ L+ 
Sbjct: 152 RRGALPWHVLFGLFVYVLTLATAELGLLEKLTFLQSSGLDKYGAEAFLVNFTGLVVALFG 211

Query: 121 ATVVLTVILP 127
           A VV+  + P
Sbjct: 212 AAVVVAAVAP 221

[Close]

BLAST of CSPI05G01400.1 vs. ExPASy Swiss-Prot
Match: Q6I681 (Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=ZCYB PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 4.4e-25
Identity = 56/130 (43.08%), Postives = 84/130 (64.62%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +AL  G  G+  AFK H+E  +++L SLHSWIG+  ++LY  QWI GF  +FFPGA    
Sbjct: 92  IALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTITLYGIQWIIGFVTFFFPGAAPNV 151

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLG----HSQQGLIVNFTGLLLLLYA 120
           +  +LPWHV FG+F++++A  NAELG L++   L       +  +  +VNFT L+++L+ 
Sbjct: 152 KKGVLPWHVLFGLFVYILALANAELGFLEKLTFLESSGLDKYGTEAFLVNFTALVVVLFG 211

Query: 121 ATVVLTVILP 127
           A+VV+  I P
Sbjct: 212 ASVVVAAIAP 221

[Close]

BLAST of CSPI05G01400.1 vs. ExPASy Swiss-Prot
Match: C4IYS8 (Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 7.5e-25
Identity = 55/130 (42.31%), Postives = 84/130 (64.62%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +AL  G  G+  AFK H+E  +++L SLHSWIG+  ++LY  QWI GF  +FFPGA    
Sbjct: 92  IALVLGAVGIYFAFKNHNESGIANLYSLHSWIGIGTITLYGIQWIVGFVTFFFPGAAPNV 151

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLG----HSQQGLIVNFTGLLLLLYA 120
           +  +LPWH+ FG+F++++A  NAELG L++   L       +  +  +VNFT L+++L+ 
Sbjct: 152 KKGVLPWHILFGLFVYILALANAELGFLEKLTFLESSGLDKYGTEAFLVNFTALVVVLFG 211

Query: 121 ATVVLTVILP 127
           A+VV+  I P
Sbjct: 212 ASVVVAAIAP 221

[Close]

BLAST of CSPI05G01400.1 vs. ExPASy Swiss-Prot
Match: A3A9H6 (Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0642300 PE=3 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.3e-24
Identity = 54/130 (41.54%), Postives = 83/130 (63.85%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +AL  G  G+  AFKFH+E  +++L SLHSW+G+  + LY  QWIFGF  +FFP A    
Sbjct: 90  IALVFGAVGIYCAFKFHNESGIANLYSLHSWLGIGTICLYGIQWIFGFVAFFFPRASPSV 149

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLG----HSQQGLIVNFTGLLLLLYA 120
           R  +LPWH+ FG+F++++A   AELG L++   L       +  +  +VNFT L+++L+ 
Sbjct: 150 RKGVLPWHILFGLFVYILALATAELGFLEKLTFLQSSGLDKYGAEAFLVNFTALIVVLFG 209

Query: 121 ATVVLTVILP 127
           A+VV+  + P
Sbjct: 210 ASVVVAAVSP 219

[Close]

BLAST of CSPI05G01400.1 vs. ExPASy TrEMBL
Match: A0A0A0KLE5 (Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G059150 PE=4 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 9.0e-66
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR
Sbjct: 32  MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 91

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV
Sbjct: 92  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 151

Query: 121 LTVILPPLY 130
           LTVILPPLY
Sbjct: 152 LTVILPPLY 160

[Close]

BLAST of CSPI05G01400.1 vs. ExPASy TrEMBL
Match: A0A5D3E074 (Transmembrane ascorbate ferrireductase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold120G002020 PE=4 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 5.0e-64
Identity = 125/129 (96.90%), Postives = 128/129 (99.22%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           MALA GIFGVCMAFKFHHEIKLSDLLSLHSWIGLIA+SLYAFQWIFGFFVYFFPGAE+RR
Sbjct: 23  MALASGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAISLYAFQWIFGFFVYFFPGAESRR 82

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           RGNMLPWHVYFG+FIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV
Sbjct: 83  RGNMLPWHVYFGIFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 142

Query: 121 LTVILPPLY 130
           LTVILPPLY
Sbjct: 143 LTVILPPLY 151

[Close]

BLAST of CSPI05G01400.1 vs. ExPASy TrEMBL
Match: A0A1S3BZN1 (transmembrane ascorbate ferrireductase 1-like OS=Cucumis melo OX=3656 GN=LOC103495163 PE=4 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 5.0e-64
Identity = 125/129 (96.90%), Postives = 128/129 (99.22%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           MALA GIFGVCMAFKFHHEIKLSDLLSLHSWIGLIA+SLYAFQWIFGFFVYFFPGAE+RR
Sbjct: 23  MALASGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAISLYAFQWIFGFFVYFFPGAESRR 82

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           RGNMLPWHVYFG+FIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV
Sbjct: 83  RGNMLPWHVYFGIFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 142

Query: 121 LTVILPPLY 130
           LTVILPPLY
Sbjct: 143 LTVILPPLY 151

[Close]

BLAST of CSPI05G01400.1 vs. ExPASy TrEMBL
Match: A0A6J1DA84 (probable ascorbate-specific transmembrane electron transporter 1 OS=Momordica charantia OX=3673 GN=LOC111018308 PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 3.8e-56
Identity = 106/128 (82.81%), Postives = 119/128 (92.97%), Query Frame = 0


Query: 2   ALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARRR 61
           ALACGIFGVC+AFKFHH+  LSDLLSLHSW GLI +SLYA QW+FGFF YFFPGAE+RRR
Sbjct: 95  ALACGIFGVCLAFKFHHDTHLSDLLSLHSWFGLITISLYALQWVFGFFAYFFPGAESRRR 154

Query: 62  GNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVVL 121
            +++PWH+YFG+FIFL+A C AELGLLQRFNTL+LGHSQ+GLIVNFTGLLLLLYAATVVL
Sbjct: 155 ASIMPWHIYFGIFIFLMAICTAELGLLQRFNTLILGHSQEGLIVNFTGLLLLLYAATVVL 214

Query: 122 TVILPPLY 130
           TVILPPLY
Sbjct: 215 TVILPPLY 222

[Close]

BLAST of CSPI05G01400.1 vs. ExPASy TrEMBL
Match: A0A6J1FVA1 (probable ascorbate-specific transmembrane electron transporter 1 OS=Cucurbita moschata OX=3662 GN=LOC111447173 PE=4 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 4.2e-47
Identity = 91/130 (70.00%), Postives = 113/130 (86.92%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +ALA  IFG+CM+FKFHH+I+L++L+SLHSW GLI V LY  QW+ GFF YFFPGAE+RR
Sbjct: 93  IALAAAIFGICMSFKFHHDIQLTNLVSLHSWFGLITVCLYGLQWVVGFFTYFFPGAESRR 152

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNT-LVLGHSQQGLIVNFTGLLLLLYAATV 120
           R +++PWHVYFG+F+FL+ATC AELGLLQ  +T  +LGH+Q+ LI+NFTGLL+ LYAATV
Sbjct: 153 RASLMPWHVYFGIFVFLMATCTAELGLLQSLDTWFLLGHTQENLIINFTGLLIFLYAATV 212

Query: 121 VLTVILPPLY 130
           VLTVILPPLY
Sbjct: 213 VLTVILPPLY 222

[Close]

BLAST of CSPI05G01400.1 vs. NCBI nr
Match: KAE8648229.1 (hypothetical protein Csa_018523 [Cucumis sativus])

HSP 1 Score: 259.2 bits (661), Expect = 1.9e-65
Identity = 129/129 (100.00%), Postives = 129/129 (100.00%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR
Sbjct: 91  MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 150

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV
Sbjct: 151 RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 210

Query: 121 LTVILPPLY 130
           LTVILPPLY
Sbjct: 211 LTVILPPLY 219

[Close]

BLAST of CSPI05G01400.1 vs. NCBI nr
Match: XP_008454856.1 (PREDICTED: transmembrane ascorbate ferrireductase 1-like [Cucumis melo] >KAA0056445.1 transmembrane ascorbate ferrireductase 1-like [Cucumis melo var. makuwa] >TYK29079.1 transmembrane ascorbate ferrireductase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 253.4 bits (646), Expect = 1.0e-63
Identity = 125/129 (96.90%), Postives = 128/129 (99.22%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           MALA GIFGVCMAFKFHHEIKLSDLLSLHSWIGLIA+SLYAFQWIFGFFVYFFPGAE+RR
Sbjct: 23  MALASGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAISLYAFQWIFGFFVYFFPGAESRR 82

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           RGNMLPWHVYFG+FIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV
Sbjct: 83  RGNMLPWHVYFGIFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 142

Query: 121 LTVILPPLY 130
           LTVILPPLY
Sbjct: 143 LTVILPPLY 151

[Close]

BLAST of CSPI05G01400.1 vs. NCBI nr
Match: XP_022150036.1 (probable ascorbate-specific transmembrane electron transporter 1 [Momordica charantia])

HSP 1 Score: 227.3 bits (578), Expect = 7.9e-56
Identity = 106/128 (82.81%), Postives = 119/128 (92.97%), Query Frame = 0


Query: 2   ALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARRR 61
           ALACGIFGVC+AFKFHH+  LSDLLSLHSW GLI +SLYA QW+FGFF YFFPGAE+RRR
Sbjct: 95  ALACGIFGVCLAFKFHHDTHLSDLLSLHSWFGLITISLYALQWVFGFFAYFFPGAESRRR 154

Query: 62  GNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVVL 121
            +++PWH+YFG+FIFL+A C AELGLLQRFNTL+LGHSQ+GLIVNFTGLLLLLYAATVVL
Sbjct: 155 ASIMPWHIYFGIFIFLMAICTAELGLLQRFNTLILGHSQEGLIVNFTGLLLLLYAATVVL 214

Query: 122 TVILPPLY 130
           TVILPPLY
Sbjct: 215 TVILPPLY 222

[Close]

BLAST of CSPI05G01400.1 vs. NCBI nr
Match: KAG6599883.1 (Transmembrane ascorbate ferrireductase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030568.1 Transmembrane ascorbate ferrireductase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 197.2 bits (500), Expect = 8.7e-47
Identity = 91/130 (70.00%), Postives = 113/130 (86.92%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +ALA  IFG+CM+FKFHH+I+L++L+SLHSW GLI V LY  QW+ GFF YFFPGAE+RR
Sbjct: 93  IALAAAIFGICMSFKFHHDIQLTNLVSLHSWFGLITVCLYGLQWVVGFFAYFFPGAESRR 152

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNT-LVLGHSQQGLIVNFTGLLLLLYAATV 120
           R +++PWHVYFG+F+FL+ATC AELGLLQ  +T  +LGH+Q+ LI+NFTGLL+ LYAATV
Sbjct: 153 RASLMPWHVYFGIFVFLMATCTAELGLLQSLDTWFLLGHTQENLIINFTGLLIFLYAATV 212

Query: 121 VLTVILPPLY 130
           VLTVILPPLY
Sbjct: 213 VLTVILPPLY 222

[Close]

BLAST of CSPI05G01400.1 vs. NCBI nr
Match: XP_022941965.1 (probable ascorbate-specific transmembrane electron transporter 1 [Cucurbita moschata])

HSP 1 Score: 197.2 bits (500), Expect = 8.7e-47
Identity = 91/130 (70.00%), Postives = 113/130 (86.92%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +ALA  IFG+CM+FKFHH+I+L++L+SLHSW GLI V LY  QW+ GFF YFFPGAE+RR
Sbjct: 93  IALAAAIFGICMSFKFHHDIQLTNLVSLHSWFGLITVCLYGLQWVVGFFTYFFPGAESRR 152

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNT-LVLGHSQQGLIVNFTGLLLLLYAATV 120
           R +++PWHVYFG+F+FL+ATC AELGLLQ  +T  +LGH+Q+ LI+NFTGLL+ LYAATV
Sbjct: 153 RASLMPWHVYFGIFVFLMATCTAELGLLQSLDTWFLLGHTQENLIINFTGLLIFLYAATV 212

Query: 121 VLTVILPPLY 130
           VLTVILPPLY
Sbjct: 213 VLTVILPPLY 222

[Close]

BLAST of CSPI05G01400.1 vs. TAIR 10
Match: AT4G25570.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 123.2 bits (308), Expect = 1.5e-28
Identity = 56/126 (44.44%), Postives = 83/126 (65.87%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           +ALA GIFG+C AFK H+E  + +L SLHSWIG+  +SLY FQW++ F V+FFPG     
Sbjct: 89  IALALGIFGICAAFKNHNESHIPNLYSLHSWIGIGVISLYGFQWVYSFIVFFFPGGSTNL 148

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLG----HSQQGLIVNFTGLLLLLYA 120
           +  +LPWH   G+F++++A  NA LG L++   L  G    +  +  ++NFT ++ +L+ 
Sbjct: 149 KSGLLPWHAMLGLFVYILAVGNAALGFLEKLTFLENGGLDKYGSEAFLINFTAIITILFG 208

Query: 121 ATVVLT 123
           A VVLT
Sbjct: 209 AFVVLT 214

[Close]

BLAST of CSPI05G01400.1 vs. TAIR 10
Match: AT5G38630.1 (cytochrome B561-1 )

HSP 1 Score: 96.7 bits (239), Expect = 1.5e-20
Identity = 46/130 (35.38%), Postives = 76/130 (58.46%), Query Frame = 0


Query: 2   ALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARRR 61
           A    + GV  A KFH +  + +  SLHSW+GL  + L+AFQW  GF  Y++PG     R
Sbjct: 91  AFILSLIGVWAALKFHIDKGIENFYSLHSWLGLACLFLFAFQWAAGFVTYWYPGGSRNSR 150

Query: 62  GNMLPWHVYFGVFIFLIATCNAELGLLQR-----FNTLVLGHSQQGLIVNFTGLLLLLYA 121
            +++PWHV+ G+ I+ +A   A  G+L++      N ++  +S + ++VN  G+L+L+  
Sbjct: 151 ASLMPWHVFLGISIYALALVTATTGILEKVTFLQVNQVITRYSTEAMLVNTMGVLILILG 210

Query: 122 ATVVLTVILP 127
             V+L V+ P
Sbjct: 211 GFVILGVVTP 220

[Close]

BLAST of CSPI05G01400.1 vs. TAIR 10
Match: AT1G14730.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 94.7 bits (234), Expect = 5.7e-20
Identity = 48/125 (38.40%), Postives = 71/125 (56.80%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           + L  GI G+C AF+FH ++ L D++SLHSWIGL    L   QW+FG F +  P + +  
Sbjct: 96  IGLVLGIVGICAAFRFHDKVNLKDMVSLHSWIGLTTFILLGVQWLFGAFTFLAPQSSSGT 155

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTLVLGHSQQGLIVNFTGLLLLLYAATVV 120
           R  M+PWHV  G  +  +    A  GL+QR   L    + +  ++NF GL +LL+  +V 
Sbjct: 156 RTRMMPWHVLGGRALLYMGIVAALTGLMQRATMLGQSTNAESRLINFLGLAILLFGVSVD 215

Query: 121 LTVIL 126
            +V L
Sbjct: 216 FSVAL 220

[Close]

BLAST of CSPI05G01400.1 vs. TAIR 10
Match: AT1G26100.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 83.2 bits (204), Expect = 1.7e-16
Identity = 48/131 (36.64%), Postives = 71/131 (54.20%), Query Frame = 0


Query: 1   MALACGIFGVCMAFKFHHEIKLSDLLSLHSWIGLIAVSLYAFQWIFGFFVYFFPGAEARR 60
           MALA  +FG+   F +   +  ++  SLHSW+GL++VSL+A QW+ GF  ++  G     
Sbjct: 83  MALASAVFGIWTKFHYQRGV-FANFYSLHSWMGLLSVSLFAAQWVTGFMSFWHRGEVRTT 142

Query: 61  RGNMLPWHVYFGVFIFLIATCNAELGLLQRFNTL-----VLGHSQQGLIVNFTGLLLLLY 120
           R   LPWHV+ G++ + +A   AE GLL++   L     V     + + VN  GL L L 
Sbjct: 143 RTTFLPWHVFLGLYTYGLAIATAETGLLEKLTFLQTKRNVPRRGSESMTVNGLGLGLALL 202

Query: 121 AATVVLTVILP 127
              V+   ILP
Sbjct: 203 GCIVITAAILP 212

The following BLAST results are available for this feature:

BLAST of CSPI05G01400.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L856	2.1e-27	44.44	Transmembrane ascorbate ferrireductase 1 OS=Arabidopsis thaliana OX=3702 GN=CYB5...	[more]
Q7XMK3	8.8e-26	43.08	Probable ascorbate-specific transmembrane electron transporter 2 OS=Oryza sativa...	[more]
Q6I681	4.4e-25	43.08	Ascorbate-specific transmembrane electron transporter 1 OS=Zea mays OX=4577 GN=Z...	[more]
C4IYS8	7.5e-25	42.31	Ascorbate-specific transmembrane electron transporter 2 OS=Zea mays OX=4577 PE=2...	[more]
A3A9H6	1.3e-24	41.54	Probable ascorbate-specific transmembrane electron transporter 1 OS=Oryza sativa...	[more]

BLAST of CSPI05G01400.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KLE5	9.0e-66	100.00	Cytochrome b561 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G05...	[more]
A0A5D3E074	5.0e-64	96.90	Transmembrane ascorbate ferrireductase 1-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A1S3BZN1	5.0e-64	96.90	transmembrane ascorbate ferrireductase 1-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1DA84	3.8e-56	82.81	probable ascorbate-specific transmembrane electron transporter 1 OS=Momordica ch...	[more]
A0A6J1FVA1	4.2e-47	70.00	probable ascorbate-specific transmembrane electron transporter 1 OS=Cucurbita mo...	[more]

BLAST of CSPI05G01400.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8648229.1	1.9e-65	100.00	hypothetical protein Csa_018523 [Cucumis sativus]	[more]
XP_008454856.1	1.0e-63	96.90	PREDICTED: transmembrane ascorbate ferrireductase 1-like [Cucumis melo] >KAA0056...	[more]
XP_022150036.1	7.9e-56	82.81	probable ascorbate-specific transmembrane electron transporter 1 [Momordica char...	[more]
KAG6599883.1	8.7e-47	70.00	Transmembrane ascorbate ferrireductase 1, partial [Cucurbita argyrosperma subsp....	[more]
XP_022941965.1	8.7e-47	70.00	probable ascorbate-specific transmembrane electron transporter 1 [Cucurbita mosc...	[more]

BLAST of CSPI05G01400.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25570.1	1.5e-28	44.44	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT5G38630.1	1.5e-20	35.38	cytochrome B561-1	[more]
AT1G14730.1	5.7e-20	38.40	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT1G26100.1	1.7e-16	36.64	Cytochrome b561/ferric reductase transmembrane protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006593	Cytochrome b561/ferric reductase transmembrane	SMART	SM00665	561_7	coord: 1..87 e-value: 1.6E-9 score: 47.6
IPR006593	Cytochrome b561/ferric reductase transmembrane	PFAM	PF03188	Cytochrom_B561	coord: 1..90 e-value: 2.3E-27 score: 95.8
IPR006593	Cytochrome b561/ferric reductase transmembrane	PROSITE	PS50939	CYTOCHROME_B561	coord: 1..124 score: 29.496042
None	No IPR available	GENE3D	1.20.120.1770		coord: 1..127 e-value: 3.4E-36 score: 126.5
None	No IPR available	PANTHER	PTHR10106:SF43	CYTOCHROME B561 FAMILY PROTEIN, EXPRESSED	coord: 1..126
IPR043205	Cytochrome b561/Cytochrome b reductase 1-like	PANTHER	PTHR10106	CYTOCHROME B561-RELATED	coord: 1..126

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI05G01400	CSPI05G01400	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI05G01400.1.cds1	CSPI05G01400.1.cds1	CDS
CSPI05G01400.1.cds2	CSPI05G01400.1.cds2	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI05G01400.1	CSPI05G01400.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016491	oxidoreductase activity