CSPI04G24170.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI04G24170.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	alpha/beta-Hydrolases superfamily protein
Location	Chr4: 22032231 .. 22035872 (-)
Sequence length	1464
RNA-Seq Expression	CSPI04G24170.1
Synteny	CSPI04G24170.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTCTAACGAGATACAATTGAATGAAAATGGTCTGACATTAAAGCCAGATAAGGCAAAAGTAAGAGATGTATTGGAGTTCTTACTTCCTTCGGGTTCCAGAAAGATAAAAAAGTTGATGGAATGTCCAGACGACCAAATTGAGCTTTACACCAATTTCAAAGCTCGATGGACCATTTCTGTTTCAATTTTGACCCAGAAATTCCTTTCTGCCATCGCTTCTCTTTTCACAATCTTAGTTACATTTTGGTCAATTATTCAAAAGTTTTGCTACAAGTATGTGCTGGCAGCAGAATTTTGGGTTGCGCCATCTCGGATTATTAATTTCACGGATGTGCAGCCCAGAGTAAGATGTAGTGACTGGGAATTGCTGGTTCCCGACGACAATGCAGACATGAATTCACAAGACCGTGATTTCAAATATTATAGTGCCTTAACCATTATGGCTTCCAAATTGGCATATCAAGATTATTCACGGTCCGCCTCCATCGTTGAGTTCGTAGTAAATGACTGCTGGCAGGTACTCCAAAATTAATTTTAATTTTAACTTTTAAGTAGTTGTTTTAAGTGTTCGGTTGAGTTATTTATAAGTTAGAAATTTTGTTTTAGTTTAAGAAAAAGTTACAGAATTTATATGTTTATCCTGAATTAACCCAATTATAGTAAGTTGGTTTTGTTCAAATAATTGTAGGTAAACATTTTAATTGATCGATTACCAAACAAAATTAAGGTCTTGTTTAAGGTCTTGTTTGCTAATCTATTTTAGTTTTAGCTTTATCTTGATTGGCATCTAATTCCTTAAATATAATAATTGAGTTTTTAGCTAAATTTTAAAAACAAAAACTAGTACAAAGGGATTATGAAATAGAAGTTAAGTAATTTTAAAGAAAAACAGCCAACCCTAATGTTAAATTCTGATTGGAATATATAAACTGATGAGTTTTCTTCTTCTCTTTCTGTTTCATTAGTGAATAATATTCATATGCAAGTAAACTTTGCAATATTTATAAACTTTGGGTAATTGGTTATTTGTTATTAGTTTTTCAGAGAACAAAAATTATTTTATAGCTATTTTTGATAAATACGTGCAGATATGTATTTAGAATTTCGTATGTATTGTTCATTTTATGTTAAGTCTTGTAGTTTTTCTATTTAATCTCATCTCATTTGTAATATTTTACAAGAACACTGAATGCAATATTCAAATCTTCTCTCAAACTTGATACAAATCCAATCCATGATGTTATTTTTTGGTTTTCAAATGCAGATGAAATTAATTGATTGTCGCAATTTCTGGAACGGTAAGATTTTTTTTTTTTTTGAACTTCTAATTATCAAATTAAATATAAATAAGGACTTTGGAATTCTCTTTTTCAAATAAATAAAGACTCTGAAATGAACTTCCTACTTTTCTAACTAAATAAAGACTGTTGACTTCTATATAATTTTGAATAAATAAATACTTAGCTTGGTCTACTTTTTTAGTTGTATTATAAAAAAAATGTATTTATATTATCAATCATAATAATATTAAAACTTAGGTGTACCTTGTTTAATTTACTATTTTTTAGATTTTAGGGTTCGGAAAGATATTAAAGCTCTTGCCCATCTTTGAGCAAGCAGATCCTCCCCTTTGTTCTCAAACTATACAGCCAATCTTAATATCAACATTTTTATTCAATTCTACGGATTATGGGGTTTTCTTGGGTTTCTCTCTAGCCATTGATTTTATTTTCGTTGCTTCTATTAATGAAGGGGTAATGATGATGATGCTATGGAGATGTCCACCTAGTGGAGATGTTCGAGTGCACCTGTTGACCACATCATATTTTTTTTATAAAAAAACTATTTTTTAGAGTTGACCATGAATCTACATTTTATCTTCTTATTTCAATATATATATTTTAAATTAAGATATTGTTTGGTATATGTACTTTTAGTTTTTGAAAATTGAGTGATAGCAACCTTTTTACATTTTCTTATTATATAAAAGGAAATGATGATAGTGTTATAAATGTCTACCTAGTGGAGATGTTCTAGTGCACGTACTTACTAATATTGTGTTTTCTTTTGAAAAAATAAGCTTTTTAATTTTTTTTGGTCAAGTAAGCATTTATATTTGTTGAATTCTTATCTAAATTCGGGAAAAGAAAAGAATGTTTTTTAAAAACTACATATTTTGCAGACTTTCAAAACAAAGCTACGACTCATGCAATTATGTTTGAGAACACACACAAAGATCCAAACGTAACAGTGATTGCATTCAGAGGGACATCCGTACTTGACATTAACGATTGGATGGTTGATCTGGACTTTTCTTGGTTCCTATTAGAAGGCAAGGTCGGCATCCACTCCGGGTTCATGCAAGCCCTTGGCTATCAAAAGTCCGGTGGTTGGCCCAAAGAACTCACCGATCCCAAACATGAATTTGCATATTACTTCCTTCGCCAAAACCTAAGAGAAATTGCTAAATCCAATGACAATGCAAAATTCATCATCACTGGCCATAGTTTGGGTGGTGCCCTTGCTACCCTTTTTGTCACTTTGTTAGCCTACCACAATGAAACAATCCTTCTTGACAAGATTCAAGCTGTTTATACCTTTGGTCAGCCTAGAGTTGGGAATCAAAGTTTTGCTCAGTTTATGGTCGACACTTTCAAAACTCATGACATTAAATATTATAGATATGTTTACTCTTTTGATTTGGTTCCTAGAATTCCTTTCCATTCTCTTGCTAATTTCAGCTACAGACACTTTGGTGGATGCGTCTACTTTGACGTCTTCTACAATGGCAAGGTGACTCTCTACCTATTTGATCATTTATTCGATTTTTTCGTGGTATTAACAACACATTTGTTCAATAATATATTTGTCTCCTTCTCCATCAACACTTAAGCTTTTGAATCAATTCCTTGTTTAAAATGGTATCATAGAACGTGGTCTCTAAAGAGAGGTGATCTTAGACCATAATGCTTTGATATATCATCTCAAGACTTAAATCACTAATTGATACAAAAGCTTTGGACTATACGGATGGAGACAAATTTAATATAAAATATAAGAGAATTGTTGCGTAAATTAATTAAATATATATTTTGGTTCATATAACAAATTTAATATAAAATATAAGAGAATTGTTGTGTAAATTAATTAAATGTATATTTTGGTTCATATAACAAAAGTTGTTGGATGTGACAATAATCTATATCATTGCTACACTGAAATCTACATTATATTATATATTGTGATGGTATATCTTTGAATTTAATTTCTTTTATTTTCCATAATTAAAGAGTGAATAAGTTCTTTTTTTTCTTTTTTTTGGTGTTTTATTTGTATGCAGTTTTTGAAGAAGCAGCCAAACACAAACTACTTTTCACTAATATGGGTTATACCCAAATACTTGAGTGCAGATTGGGAGTTCATTAGAAGTTTAATAATAACCCCAATTGTGAAGGGTAGAAAGTACTTCGAGGGTTTTTTTACAATAATGGAAAGGACAGTTGGGTTGGTTATTCCGGGAATATCAGCTCATGTATGTTCAAATTATGTCAGCCTGACTCGTTGGGGTAATATACATTTGCCACCTGACCACCACGAACTCTTGAAGTTTGCTCACTACATTGAAGCTGACTACTAG

mRNA sequence

ATGGGTTCTAACGAGATACAATTGAATGAAAATGGTCTGACATTAAAGCCAGATAAGGCAAAAGTAAGAGATGTATTGGAGTTCTTACTTCCTTCGGGTTCCAGAAAGATAAAAAAGTTGATGGAATGTCCAGACGACCAAATTGAGCTTTACACCAATTTCAAAGCTCGATGGACCATTTCTGTTTCAATTTTGACCCAGAAATTCCTTTCTGCCATCGCTTCTCTTTTCACAATCTTAGTTACATTTTGGTCAATTATTCAAAAGTTTTGCTACAAGTATGTGCTGGCAGCAGAATTTTGGGTTGCGCCATCTCGGATTATTAATTTCACGGATGTGCAGCCCAGAGTAAGATGTAGTGACTGGGAATTGCTGGTTCCCGACGACAATGCAGACATGAATTCACAAGACCGTGATTTCAAATATTATAGTGCCTTAACCATTATGGCTTCCAAATTGGCATATCAAGATTATTCACGGTCCGCCTCCATCGTTGAGTTCGTAGTAAATGACTGCTGGCAGATGAAATTAATTGATTGTCGCAATTTCTGGAACGACTTTCAAAACAAAGCTACGACTCATGCAATTATGTTTGAGAACACACACAAAGATCCAAACGTAACAGTGATTGCATTCAGAGGGACATCCGTACTTGACATTAACGATTGGATGGTTGATCTGGACTTTTCTTGGTTCCTATTAGAAGGCAAGGTCGGCATCCACTCCGGGTTCATGCAAGCCCTTGGCTATCAAAAGTCCGGTGGTTGGCCCAAAGAACTCACCGATCCCAAACATGAATTTGCATATTACTTCCTTCGCCAAAACCTAAGAGAAATTGCTAAATCCAATGACAATGCAAAATTCATCATCACTGGCCATAGTTTGGGTGGTGCCCTTGCTACCCTTTTTGTCACTTTGTTAGCCTACCACAATGAAACAATCCTTCTTGACAAGATTCAAGCTGTTTATACCTTTGGTCAGCCTAGAGTTGGGAATCAAAGTTTTGCTCAGTTTATGGTCGACACTTTCAAAACTCATGACATTAAATATTATAGATATGTTTACTCTTTTGATTTGGTTCCTAGAATTCCTTTCCATTCTCTTGCTAATTTCAGCTACAGACACTTTGGTGGATGCGTCTACTTTGACGTCTTCTACAATGGCAAGTTTTTGAAGAAGCAGCCAAACACAAACTACTTTTCACTAATATGGGTTATACCCAAATACTTGAGTGCAGATTGGGAGTTCATTAGAAGTTTAATAATAACCCCAATTGTGAAGGGTAGAAAGTACTTCGAGGGTTTTTTTACAATAATGGAAAGGACAGTTGGGTTGGTTATTCCGGGAATATCAGCTCATGTATGTTCAAATTATGTCAGCCTGACTCGTTGGGGTAATATACATTTGCCACCTGACCACCACGAACTCTTGAAGTTTGCTCACTACATTGAAGCTGACTACTAG

Coding sequence (CDS)

ATGGGTTCTAACGAGATACAATTGAATGAAAATGGTCTGACATTAAAGCCAGATAAGGCAAAAGTAAGAGATGTATTGGAGTTCTTACTTCCTTCGGGTTCCAGAAAGATAAAAAAGTTGATGGAATGTCCAGACGACCAAATTGAGCTTTACACCAATTTCAAAGCTCGATGGACCATTTCTGTTTCAATTTTGACCCAGAAATTCCTTTCTGCCATCGCTTCTCTTTTCACAATCTTAGTTACATTTTGGTCAATTATTCAAAAGTTTTGCTACAAGTATGTGCTGGCAGCAGAATTTTGGGTTGCGCCATCTCGGATTATTAATTTCACGGATGTGCAGCCCAGAGTAAGATGTAGTGACTGGGAATTGCTGGTTCCCGACGACAATGCAGACATGAATTCACAAGACCGTGATTTCAAATATTATAGTGCCTTAACCATTATGGCTTCCAAATTGGCATATCAAGATTATTCACGGTCCGCCTCCATCGTTGAGTTCGTAGTAAATGACTGCTGGCAGATGAAATTAATTGATTGTCGCAATTTCTGGAACGACTTTCAAAACAAAGCTACGACTCATGCAATTATGTTTGAGAACACACACAAAGATCCAAACGTAACAGTGATTGCATTCAGAGGGACATCCGTACTTGACATTAACGATTGGATGGTTGATCTGGACTTTTCTTGGTTCCTATTAGAAGGCAAGGTCGGCATCCACTCCGGGTTCATGCAAGCCCTTGGCTATCAAAAGTCCGGTGGTTGGCCCAAAGAACTCACCGATCCCAAACATGAATTTGCATATTACTTCCTTCGCCAAAACCTAAGAGAAATTGCTAAATCCAATGACAATGCAAAATTCATCATCACTGGCCATAGTTTGGGTGGTGCCCTTGCTACCCTTTTTGTCACTTTGTTAGCCTACCACAATGAAACAATCCTTCTTGACAAGATTCAAGCTGTTTATACCTTTGGTCAGCCTAGAGTTGGGAATCAAAGTTTTGCTCAGTTTATGGTCGACACTTTCAAAACTCATGACATTAAATATTATAGATATGTTTACTCTTTTGATTTGGTTCCTAGAATTCCTTTCCATTCTCTTGCTAATTTCAGCTACAGACACTTTGGTGGATGCGTCTACTTTGACGTCTTCTACAATGGCAAGTTTTTGAAGAAGCAGCCAAACACAAACTACTTTTCACTAATATGGGTTATACCCAAATACTTGAGTGCAGATTGGGAGTTCATTAGAAGTTTAATAATAACCCCAATTGTGAAGGGTAGAAAGTACTTCGAGGGTTTTTTTACAATAATGGAAAGGACAGTTGGGTTGGTTATTCCGGGAATATCAGCTCATGTATGTTCAAATTATGTCAGCCTGACTCGTTGGGGTAATATACATTTGCCACCTGACCACCACGAACTCTTGAAGTTTGCTCACTACATTGAAGCTGACTACTAG

Protein sequence

MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTISVSILTQKFLSAIASLFTILVTFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCSDWELLVPDDNADMNSQDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGIHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSADWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWGNIHLPPDHHELLKFAHYIEADY*

Homology

[Close]

BLAST of CSPI04G24170.1 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 8.0e-64
Identity = 130/338 (38.46%), Postives = 195/338 (57.69%), Query Frame = 0


Query: 146 LTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDCRNFWNDFQNKATTHAIMFENTHKDP 205
           L +MASKLAY+    +A +VE VV+  W+M L++  + WND+Q + +T   +F +  KD 
Sbjct: 177 LCVMASKLAYE----NAKVVENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDA 236

Query: 206 NVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGIHSGFMQALGY--------------- 265
           N+ VI+FRGT   D +DW  D D+SW+ +     +H GF++A+G                
Sbjct: 237 NLIVISFRGTEPFDADDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFE 296

Query: 266 ---QKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLL 325
               +     K L D     AYY +R  L+ +   ++NA+F++TGHSLGGALA LF TLL
Sbjct: 297 QTSSEEENSKKNLLDMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLL 356

Query: 326 AYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLVPRIPFHS 385
             + ET ++ ++  VYTFGQPR+GN+    FM         +Y+R VY  D+VPR+P+  
Sbjct: 357 VLNEETEIMKRLLGVYTFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDD 416

Query: 386 LANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSADWEFIRSLIITPIVK 445
              F Y+HFG C+++D FYN    + +P+ N + L + I  ++ A WE +R L +     
Sbjct: 417 -KTFLYKHFGLCLFYDSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTM-GYTH 476

Query: 446 GRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWG 466
           G  Y EG+F I+ R +GLVIPG+S H  ++YV+  R G
Sbjct: 477 GPDYKEGWFRILFRLMGLVIPGLSDHCMTDYVNSVRLG 508

[Close]

BLAST of CSPI04G24170.1 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 3.5e-59
Identity = 129/379 (34.04%), Postives = 197/379 (51.98%), Query Frame = 0


Query: 146 LTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDCRNFWNDFQNKATTHAIMFENTHKDP 205
           L +MASKLAY+    +  +V  VVN  W+M  +D  N WNDF+ + +T   +  +  KD 
Sbjct: 190 LCMMASKLAYE----NEKVVRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDA 249

Query: 206 NVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGIHSGFMQALGYQKSGGW--------- 265
           N+ +++FRGT   D +DW+ D D+SW+ +     +H GF++ALG                
Sbjct: 250 NLILVSFRGTEPFDADDWITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFV 309

Query: 266 ----------------PKELTDPKHEF--------------------------------- 325
                           P E +     F                                 
Sbjct: 310 NNLKFANLENVHATIPPSESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPER 369

Query: 326 -AYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFG 385
            AYY +R  L+ + K + NAKF++TGHSLGGALA LF  +L  H E  +++++  +YT+G
Sbjct: 370 TAYYVVRSKLKRLLKEHKNAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYG 429

Query: 386 QPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFY 445
           QPRVGN+   +FM    +    KY+R VY  DLVPR+P+ +   F ++HFG C Y++  Y
Sbjct: 430 QPRVGNRQLGRFMEAHLEHPVPKYFRVVYCNDLVPRLPYDN-KTFLFKHFGICQYYNSLY 489

Query: 446 NGKFLKKQPNTNYFSLIWVIPKYLSADWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLV 466
             + + ++PN NYF + +++P YL+A WE IRS  +   + G +Y E + ++M R +GL 
Sbjct: 490 IEQNINEEPNPNYFGMRFLVPLYLNAGWELIRSFTM-GYMYGSEYEECWESVMLRALGLF 549

[Close]

BLAST of CSPI04G24170.1 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 5.1e-58
Identity = 164/506 (32.41%), Postives = 246/506 (48.62%), Query Frame = 0


Query: 13  LTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTISVSI-----LTQ 72
           L + PD+    D+ + ++ S      K     D ++     + +RW +SV I     L Q
Sbjct: 13  LIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRWPVSVFICKILQLFQ 72

Query: 73  KFLSAIASLFTILVTFWSIIQKFCYKYVLAAEFWV---APSR-------IINFTDVQ--- 132
              + +  L   L+ F+   Q   +  +L   F +    P R        I + D +   
Sbjct: 73  MPAAMLGHLTDFLLNFY--YQNHGFLGILRNIFLIRLKIPKRGEADFISTIGYLDSRMDL 132

Query: 133 ---PRVRCSDWELLVPDDNADMNSQDRDFKYYSA--------LTIMASKLAYQDYSRSAS 192
              P V     E++   DN  +  +  + K   A        L IMASKLAY+    +  
Sbjct: 133 HGTPMVSHQADEVISNADNPSL-KEGHNSKIKGALGNRSLMDLCIMASKLAYE----NTK 192

Query: 193 IVEFVVNDCWQMKLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDW 252
           +VE VV + W+M  +      N FQ+   THA +F +  KD N+ VI+FRGT    I +W
Sbjct: 193 VVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIPNW 252

Query: 253 MVDLDFSWFLLEGKVGIHSGFMQALGY--------------QKSGGWPKELTD-----PK 312
             D DFS   L     +H GF++A+G                KS G   EL       P 
Sbjct: 253 CTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEMAPD 312

Query: 313 H-----EFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETILLDKI 372
           H     +  Y+     L+ + K + NAKF++TGHSLGGALA LF  +L    ET +LD++
Sbjct: 313 HLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVLDRL 372

Query: 373 QAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLVPRIPFHSLANFSYRHFGGC 432
             VYTFGQPR+GN +   FM +     + +Y+R VY  D+VPR+PF  +  F++ HFG C
Sbjct: 373 LNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVF-FTFEHFGTC 432

Query: 433 VYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSADWEFIRSLIITPIVKGRKYFEGFFTIM 466
           +Y+D  + G F K++P+ N F +   I  +++A WE  RS I+   V G +Y E + + M
Sbjct: 433 IYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNH-VYGAEYKETWESRM 492

[Close]

BLAST of CSPI04G24170.1 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 8.7e-10
Identity = 71/248 (28.63%), Postives = 119/248 (47.98%), Query Frame = 0


Query: 132 DMNSQDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDCRNFWNDFQNKA 191
           D+ +Q   F  YSA    A+     + + + + +    N C +++  D   F   F++  
Sbjct: 27  DLFNQFNLFAQYSA----AAYCGKNNDAPAGTNITCTGNACPEVEKADA-TFLYSFEDSG 86

Query: 192 ---TTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGIHSGFMQAL 251
               T  +  +NT+K   + V++FRG+    I +W+ +L+F    L+    I SG     
Sbjct: 87  VGDVTGFLALDNTNK---LIVLSFRGSR--SIENWIGNLNFD---LKEINDICSGCRGHD 146

Query: 252 GYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLA 311
           G+  S  W + + D         LRQ + +  + + + + + TGHSLGGALAT+    L 
Sbjct: 147 GFTSS--W-RSVADT--------LRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLR 206

Query: 312 YHNETILLDKIQAVYTFGQPRVGNQSFAQFM-VDTFKTHDIKYYRYVYSFDLVPRIPFHS 371
            +   I       V+++G PRVGN++FA+F+ V T  T     YR  ++ D+VPR+P   
Sbjct: 207 GNGYDI------DVFSYGAPRVGNRAFAEFLTVQTGGT----LYRITHTNDIVPRLP--- 237

Query: 372 LANFSYRH 376
              F Y H
Sbjct: 267 PREFGYSH 237

[Close]

BLAST of CSPI04G24170.1 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 1.1e-09
Identity = 59/171 (34.50%), Postives = 80/171 (46.78%), Query Frame = 0


Query: 210 IAFRGTSVLDINDWMVDLDF---SWFLLEGKVGIHSGFMQALGYQKSGGWPKELTDPKHE 269
           I FRG+S   I +W+ DL F   S+  + G   +H GF+ + G            + ++E
Sbjct: 171 IVFRGSS--SIRNWIADLTFVPVSYPPVSG-TKVHKGFLDSYG------------EVQNE 230

Query: 270 FAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFG 329
                L Q      K   + K  +TGHSLGGA A L   L  Y  E  L      +YT G
Sbjct: 231 LVATVLDQ-----FKQYPSYKVAVTGHSLGGATA-LLCALDLYQREEGLSSSNLFLYTQG 290

Query: 330 QPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLVPRIPFHSLANFSYRHFG 378
           QPRVG+ +FA ++V T     I Y R V   D+VP +P    A F + H G
Sbjct: 291 QPRVGDPAFANYVVST----GIPYRRTVNERDIVPHLP---PAAFGFLHAG 313

[Close]

BLAST of CSPI04G24170.1 vs. ExPASy TrEMBL
Match: A0A0A0KZS7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639880 PE=4 SV=1)

HSP 1 Score: 1003.0 bits (2592), Expect = 4.2e-289
Identity = 483/487 (99.18%), Postives = 485/487 (99.59%), Query Frame = 0


Query: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60
           MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI
Sbjct: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60

Query: 61  SVSILTQKFLSAIASLFTILVTFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 120
           SVSILTQKFLSAIASLFTILV FWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS
Sbjct: 61  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 120

Query: 121 DWELLVPDDNADMNSQDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 180
           DWELLVPDDNADMNS DRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC
Sbjct: 121 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 180

Query: 181 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 240
           RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI
Sbjct: 181 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 240

Query: 241 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300
           HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA
Sbjct: 241 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300

Query: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 360
           TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV
Sbjct: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 360

Query: 361 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSADWEFIRSL 420
           PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLK+QPNTNYFSLIWVIPKYLSADWEFIRSL
Sbjct: 361 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL 420

Query: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWGNIHLPPDHHELLKFA 480
           IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYV+LTRWGNIHLPPDHHELLKFA
Sbjct: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA 480

Query: 481 HYIEADY 488
           HYIEADY
Sbjct: 481 HYIEADY 487

[Close]

BLAST of CSPI04G24170.1 vs. ExPASy TrEMBL
Match: A0A6J1FDN5 (uncharacterized protein LOC111443149 OS=Cucurbita moschata OX=3662 GN=LOC111443149 PE=4 SV=1)

HSP 1 Score: 613.2 bits (1580), Expect = 9.3e-172
Identity = 299/487 (61.40%), Postives = 367/487 (75.36%), Query Frame = 0


Query: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60
           MGS + + +E+ L LKP +A + DV  F+LP GSR+I+ L++CPD + E Y++FKAR TI
Sbjct: 3   MGSKQNEFSEDYLILKPKEASLVDVFLFILPLGSRRIRSLIDCPDAKEESYSSFKARCTI 62

Query: 61  SVSILTQKFLSAIASLFTILVTFWSIIQKFCYKYVLA-AEFWVAPSRIINFTDVQPRVRC 120
            VSIL QKFL A+A+    L  F S+IQ   + Y++   EF+         +D  P VRC
Sbjct: 63  FVSILVQKFLLALAAPLLTLRAFLSVIQPLFFNYMIGKGEFYTR-------SDSGPEVRC 122

Query: 121 SDWELLVPDDNADMNSQDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLID 180
            DW++  PD        D+ FKYY ALT+MAS+LAY+DY+ S S+V  VVN CWQM L+ 
Sbjct: 123 RDWQIEDPD--------DKGFKYYGALTMMASQLAYEDYTASPSVVHSVVNGCWQMTLLG 182

Query: 181 CRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVG 240
           C NFWNDFQNKATT A MF+NT KDPNVTV+AFRGTSV + +DWMVD + SW+ +EG   
Sbjct: 183 CFNFWNDFQNKATTGAFMFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWYKIEGIGR 242

Query: 241 IHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGAL 300
           IHSGFMQALG QK+ GWPKEL + +HEFAYY LR+ LR+I KSND AKFIITGHSLGGAL
Sbjct: 243 IHSGFMQALGLQKATGWPKELPEAEHEFAYYTLRRRLRDIVKSNDKAKFIITGHSLGGAL 302

Query: 301 ATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDL 360
           ATLFVTLLA H ++ +L ++QAVYTFGQPRVG++ FA+FMV+T   + +KYYRYVYSFDL
Sbjct: 303 ATLFVTLLALHQDSTILQRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGLKYYRYVYSFDL 362

Query: 361 VPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSADWEFIRS 420
           VPR+PF S+  F Y+HFGGC+YFD FYNGKF K+QPNTNYFSLIWVIPKY SA WE I S
Sbjct: 363 VPRVPFDSII-FKYKHFGGCLYFDCFYNGKFRKEQPNTNYFSLIWVIPKYSSAHWELISS 422

Query: 421 LIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWGNIHLPPDHHELLKF 480
           LII P+ KGR+YFEGF T +ER VGL  PG+SAH+C NYV++TRWG I + PD +E LK 
Sbjct: 423 LIIIPVFKGREYFEGFVTTLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDRNETLKL 473

Query: 481 AHYIEAD 487
             YIEAD
Sbjct: 483 VRYIEAD 473

[Close]

BLAST of CSPI04G24170.1 vs. ExPASy TrEMBL
Match: A0A6J1CEU2 (uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010582 PE=4 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 1.3e-138
Identity = 253/466 (54.29%), Postives = 315/466 (67.60%), Query Frame = 0


Query: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60
           MGS E + + + L LKP++A +  +  F LP G RK++ L++CP  +   YT F  RW I
Sbjct: 1   MGSQENKFSSDYLILKPEEATLLHLFLFTLPFGYRKLRNLVDCPVAKENSYTTFGNRWVI 60

Query: 61  SVSILTQKFLSAIASLFTILVTFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 120
            +SIL QKFL AIA+L     +      K C                       P+V C 
Sbjct: 61  FISILLQKFLLAIANLIKYCKSMRGKFHKTC---------------------AAPKVNCK 120

Query: 121 DWELLVPDDNADMNSQDRDFKYYSALTIMASKLAYQDY-SRSASIVEFVVNDCWQMKLID 180
           +W+++  + N  + + D  FKYY ALT+MAS LAYQDY S   S+V+ VVN CW+M L+ 
Sbjct: 121 EWKIIGMEKNIKL-ADDDTFKYYGALTVMASNLAYQDYNSPEPSVVQTVVNGCWKMNLLG 180

Query: 181 CRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVG 240
           C +FWNDFQNKATT   MF+NT  DPNVTV+AFRG+S  +I DWMVDL+ SW+ +EG   
Sbjct: 181 CYDFWNDFQNKATTQVFMFQNTATDPNVTVVAFRGSS--EIYDWMVDLNLSWYNIEGIGK 240

Query: 241 IHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGAL 300
           IH GFMQALG QK  GWPKEL +P H+FAYY LRQ LR+I KSNDNA+FI TGHSLGGAL
Sbjct: 241 IHGGFMQALGLQKGTGWPKELANPDHDFAYYTLRQQLRDIVKSNDNARFIFTGHSLGGAL 300

Query: 301 ATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDL 360
           A LF TLLA+H++  LL+K+QAVYTFGQPRVG++ FAQFM +T + +  KYYRYVYS DL
Sbjct: 301 AVLFATLLAFHDDATLLEKLQAVYTFGQPRVGDRRFAQFMENTIEKYGFKYYRYVYSSDL 360

Query: 361 VPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSADWEFIRS 420
           VPR+PF    N  Y HFGGCVYF+  YNGKFL+ QPN NYFS IW+I KY+SA WE IRS
Sbjct: 361 VPRVPFDFNDNLWYEHFGGCVYFNCSYNGKFLEVQPNKNYFSPIWLISKYVSAWWELIRS 420

Query: 421 LIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWG 466
           L+I  I     Y EGF  ++ R  GL+ PG SAH+C NY++  RWG
Sbjct: 421 LMIPVIKCSSDYLEGFSNLLLRLFGLLFPGASAHMCLNYINSIRWG 442

[Close]

BLAST of CSPI04G24170.1 vs. ExPASy TrEMBL
Match: A0A6J1C148 (uncharacterized protein LOC111007483 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007483 PE=4 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 4.8e-128
Identity = 245/474 (51.69%), Postives = 328/474 (69.20%), Query Frame = 0


Query: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60
           MG  + Q + + L LKP++A + D+  F+LP G  +++KL++CP+++ E Y +F  RW I
Sbjct: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60

Query: 61  SVSILTQKFLSAIASLFTILVTFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 120
            +SIL+QKFL AIA+L          ++K+     +  +FW    R+  +      V C 
Sbjct: 61  FISILSQKFLLAIANL----------LEKW---EPMRGKFW----RVQTYAS---EVNCR 120

Query: 121 DWELLVPDDNADMNSQDRDFKYYSALTIMASKLAYQDYS-RSASIVEFVVNDCWQMKLID 180
           DW+ +  ++N  + + D  F YY A+T+MAS L YQD S   +S+V  VVN CW+MKL+ 
Sbjct: 121 DWK-IGKEENIKLLNDDDTFVYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLG 180

Query: 181 CRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVG 240
           C +F N F++KA T A MF+NT  DPNVTV+AF+GT+   I D ++DL+FSW+ ++ ++G
Sbjct: 181 CYDFENMFESKARTQATMFQNTATDPNVTVVAFKGTNT--IYDAILDLNFSWYNVDQRIG 240

Query: 241 -IHSGFMQALGYQKSG-GWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGG 300
            IHSGFM+ALG QK   GWPKEL    H+FAYY+LRQ LR+IAKSND AKFI TGHSLGG
Sbjct: 241 NIHSGFMEALGLQKGTIGWPKELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGG 300

Query: 301 ALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSF 360
           ALA LFVT+L+YHNE+ +L+K+QAVYTFGQPRVGN  F +FM  T K +  KYYRYVYS 
Sbjct: 301 ALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFVKFMESTSKQYAFKYYRYVYSM 360

Query: 361 DLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSADWEFI 420
           DLVPRIPF     + Y+HFGGCVYF+  Y+G+F++ QPN NYFS +W+IPKYL A WE I
Sbjct: 361 DLVPRIPFDIFDEW-YKHFGGCVYFNCCYSGQFMEVQPNKNYFSPLWLIPKYLFALWELI 420

Query: 421 RSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWGNIHLPP 472
           RSL++  I     YFEGF T++ R  GL+IPG SAH+C NY+ L R G   +PP
Sbjct: 421 RSLMMPLIKFSLYYFEGFKTLLLRFFGLLIPGASAHLCVNYIYLARQGK--MPP 448

[Close]

BLAST of CSPI04G24170.1 vs. ExPASy TrEMBL
Match: A0A6J1C321 (uncharacterized protein LOC111007483 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007483 PE=4 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 6.9e-127
Identity = 249/474 (52.53%), Postives = 324/474 (68.35%), Query Frame = 0


Query: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60
           MGS E + + + L LKP +A + D+  FLLP GS K++KL++CP ++ E YT+F  RW I
Sbjct: 1   MGSLEYEFSNDYLKLKPKEATLLDLFIFLLPFGSIKLRKLVDCPPEKEESYTSFTNRWVI 60

Query: 61  SVSILTQKFLSAIASLFTILVTFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 120
             SIL+QK L A+A+L   L  + S  +K             AP    +       V C 
Sbjct: 61  FFSILSQKSLFALANL---LEKWESSTEK----------LRTAPQTYAS------EVNCR 120

Query: 121 DWELLVPDDNADMNSQDRDFKYYSALTIMASKLAYQDYS-RSASIVEFVVNDCWQMKLID 180
           DW+ +  ++N  + + D  F YY A+T+MAS L YQD S   +S+V  VVN CW+MKL+ 
Sbjct: 121 DWK-IGKEENIKLLNDDDTFVYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLG 180

Query: 181 CRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVG 240
           C +F N F++KA T A MF+NT  DPNVTV+AF+GT+   I D ++DL+FSW+ ++ ++G
Sbjct: 181 CYDFENMFESKARTQATMFQNTATDPNVTVVAFKGTNT--IYDAILDLNFSWYNVDQRIG 240

Query: 241 -IHSGFMQALGYQKSG-GWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGG 300
            IHSGFM+ALG QK   GWPKEL    H+FAYY+LRQ LR+IAKSND AKFI TGHSLGG
Sbjct: 241 NIHSGFMEALGLQKGTIGWPKELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGG 300

Query: 301 ALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSF 360
           ALA LFVT+L+YHNE+ +L+K+QAVYTFGQPRVGN  F +FM  T K +  KYYRYVYS 
Sbjct: 301 ALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFVKFMESTSKQYAFKYYRYVYSM 360

Query: 361 DLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSADWEFI 420
           DLVPRIPF     + Y+HFGGCVYF+  Y+G+F++ QPN NYFS +W+IPKYL A WE I
Sbjct: 361 DLVPRIPFDIFDEW-YKHFGGCVYFNCCYSGQFMEVQPNKNYFSPLWLIPKYLFALWELI 420

Query: 421 RSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWGNIHLPP 472
           RSL++  I     YFEGF T++ R  GL+IPG SAH+C NY+ L R G   +PP
Sbjct: 421 RSLMMPLIKFSLYYFEGFKTLLLRFFGLLIPGASAHLCVNYIYLARQGK--MPP 449

[Close]

BLAST of CSPI04G24170.1 vs. NCBI nr
Match: XP_031739748.1 (uncharacterized protein LOC105435389 [Cucumis sativus])

HSP 1 Score: 1003.0 bits (2592), Expect = 8.6e-289
Identity = 483/487 (99.18%), Postives = 485/487 (99.59%), Query Frame = 0


Query: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60
           MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI
Sbjct: 12  MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 71

Query: 61  SVSILTQKFLSAIASLFTILVTFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 120
           SVSILTQKFLSAIASLFTILV FWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS
Sbjct: 72  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 131

Query: 121 DWELLVPDDNADMNSQDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 180
           DWELLVPDDNADMNS DRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC
Sbjct: 132 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 191

Query: 181 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 240
           RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI
Sbjct: 192 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 251

Query: 241 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300
           HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA
Sbjct: 252 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 311

Query: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 360
           TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV
Sbjct: 312 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 371

Query: 361 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSADWEFIRSL 420
           PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLK+QPNTNYFSLIWVIPKYLSADWEFIRSL
Sbjct: 372 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL 431

Query: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWGNIHLPPDHHELLKFA 480
           IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYV+LTRWGNIHLPPDHHELLKFA
Sbjct: 432 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA 491

Query: 481 HYIEADY 488
           HYIEADY
Sbjct: 492 HYIEADY 498

[Close]

BLAST of CSPI04G24170.1 vs. NCBI nr
Match: KGN55200.1 (hypothetical protein Csa_012841 [Cucumis sativus])

HSP 1 Score: 1003.0 bits (2592), Expect = 8.6e-289
Identity = 483/487 (99.18%), Postives = 485/487 (99.59%), Query Frame = 0


Query: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60
           MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI
Sbjct: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60

Query: 61  SVSILTQKFLSAIASLFTILVTFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 120
           SVSILTQKFLSAIASLFTILV FWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS
Sbjct: 61  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 120

Query: 121 DWELLVPDDNADMNSQDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 180
           DWELLVPDDNADMNS DRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC
Sbjct: 121 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 180

Query: 181 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 240
           RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI
Sbjct: 181 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 240

Query: 241 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300
           HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA
Sbjct: 241 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300

Query: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 360
           TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV
Sbjct: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 360

Query: 361 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSADWEFIRSL 420
           PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLK+QPNTNYFSLIWVIPKYLSADWEFIRSL
Sbjct: 361 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL 420

Query: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWGNIHLPPDHHELLKFA 480
           IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYV+LTRWGNIHLPPDHHELLKFA
Sbjct: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA 480

Query: 481 HYIEADY 488
           HYIEADY
Sbjct: 481 HYIEADY 487

[Close]

BLAST of CSPI04G24170.1 vs. NCBI nr
Match: XP_038896694.1 (triacylglycerol lipase OBL1-like [Benincasa hispida])

HSP 1 Score: 755.0 bits (1948), Expect = 4.1e-214
Identity = 360/487 (73.92%), Postives = 415/487 (85.22%), Query Frame = 0


Query: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60
           MGSNEI+  EN L LK   AKVRDV  FLLPSGSRKIK ++ CPDDQI  YTNFKARWTI
Sbjct: 1   MGSNEIEFRENYLILKAKDAKVRDVFLFLLPSGSRKIKNVIYCPDDQIGFYTNFKARWTI 60

Query: 61  SVSILTQKFLSAIASLFTILVTFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 120
           SVSIL QKFL  +A+L T+L  FWSIIQ+F YKY+L AEFWVAP + INF D++P+VRC 
Sbjct: 61  SVSILAQKFLLTLATLLTLLAAFWSIIQRFFYKYILLAEFWVAP-QTINFMDLKPKVRCR 120

Query: 121 DWELLVPDDNADMNSQDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 180
           DW++L P+DNADMN ++RDF+YYS LTIMASKLAYQDYS+  S+VE VVNDCWQM LIDC
Sbjct: 121 DWKILDPNDNADMNLEERDFRYYSNLTIMASKLAYQDYSQYPSVVESVVNDCWQMNLIDC 180

Query: 181 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 240
            NFWNDFQ+KATTHA MFENT KDP VTV+AFRGTSVL+ +DW VD +FSW  LEG   I
Sbjct: 181 FNFWNDFQDKATTHAFMFENTSKDPKVTVVAFRGTSVLEPSDWTVDFNFSWSKLEGVGNI 240

Query: 241 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300
           H+GFMQALG+Q   GWPK+L  PKH++AYY LRQ L++IAK+NDNA+FIITGHSLGGALA
Sbjct: 241 HAGFMQALGFQDLAGWPKDLLQPKHQYAYYTLRQKLKDIAKANDNAEFIITGHSLGGALA 300

Query: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 360
           TLFVTLLA+HNET +L KI+AVYT+GQPRVGNQ FAQFMVDT + +  KYYRYVYSFDLV
Sbjct: 301 TLFVTLLAHHNETTILHKIRAVYTYGQPRVGNQEFAQFMVDTVERYGFKYYRYVYSFDLV 360

Query: 361 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSADWEFIRSL 420
           PR+PF S+  F Y+HFGGCVYFD FY+GKFLK+QPNTNYFS IW+IPKYLSA WEFIRSL
Sbjct: 361 PRVPFDSVV-FKYKHFGGCVYFDCFYHGKFLKEQPNTNYFSPIWLIPKYLSACWEFIRSL 420

Query: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWGNIHLPPDHHELLKFA 480
           IITPIVKGR YFEGFFTI+ERT+GL+IPGISAHVCSNYV++TR GNI LP D+ E L+ A
Sbjct: 421 IITPIVKGRDYFEGFFTILERTLGLLIPGISAHVCSNYVNVTRLGNIQLPSDYEETLQLA 480

Query: 481 HYIEADY 488
           HYI+A+Y
Sbjct: 481 HYIDANY 485

[Close]

BLAST of CSPI04G24170.1 vs. NCBI nr
Match: XP_022936593.1 (uncharacterized protein LOC111443149 [Cucurbita moschata])

HSP 1 Score: 613.2 bits (1580), Expect = 1.9e-171
Identity = 299/487 (61.40%), Postives = 367/487 (75.36%), Query Frame = 0


Query: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60
           MGS + + +E+ L LKP +A + DV  F+LP GSR+I+ L++CPD + E Y++FKAR TI
Sbjct: 3   MGSKQNEFSEDYLILKPKEASLVDVFLFILPLGSRRIRSLIDCPDAKEESYSSFKARCTI 62

Query: 61  SVSILTQKFLSAIASLFTILVTFWSIIQKFCYKYVLA-AEFWVAPSRIINFTDVQPRVRC 120
            VSIL QKFL A+A+    L  F S+IQ   + Y++   EF+         +D  P VRC
Sbjct: 63  FVSILVQKFLLALAAPLLTLRAFLSVIQPLFFNYMIGKGEFYTR-------SDSGPEVRC 122

Query: 121 SDWELLVPDDNADMNSQDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLID 180
            DW++  PD        D+ FKYY ALT+MAS+LAY+DY+ S S+V  VVN CWQM L+ 
Sbjct: 123 RDWQIEDPD--------DKGFKYYGALTMMASQLAYEDYTASPSVVHSVVNGCWQMTLLG 182

Query: 181 CRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVG 240
           C NFWNDFQNKATT A MF+NT KDPNVTV+AFRGTSV + +DWMVD + SW+ +EG   
Sbjct: 183 CFNFWNDFQNKATTGAFMFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWYKIEGIGR 242

Query: 241 IHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGAL 300
           IHSGFMQALG QK+ GWPKEL + +HEFAYY LR+ LR+I KSND AKFIITGHSLGGAL
Sbjct: 243 IHSGFMQALGLQKATGWPKELPEAEHEFAYYTLRRRLRDIVKSNDKAKFIITGHSLGGAL 302

Query: 301 ATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDL 360
           ATLFVTLLA H ++ +L ++QAVYTFGQPRVG++ FA+FMV+T   + +KYYRYVYSFDL
Sbjct: 303 ATLFVTLLALHQDSTILQRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGLKYYRYVYSFDL 362

Query: 361 VPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSADWEFIRS 420
           VPR+PF S+  F Y+HFGGC+YFD FYNGKF K+QPNTNYFSLIWVIPKY SA WE I S
Sbjct: 363 VPRVPFDSII-FKYKHFGGCLYFDCFYNGKFRKEQPNTNYFSLIWVIPKYSSAHWELISS 422

Query: 421 LIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWGNIHLPPDHHELLKF 480
           LII P+ KGR+YFEGF T +ER VGL  PG+SAH+C NYV++TRWG I + PD +E LK 
Sbjct: 423 LIIIPVFKGREYFEGFVTTLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDRNETLKL 473

Query: 481 AHYIEAD 487
             YIEAD
Sbjct: 483 VRYIEAD 473

[Close]

BLAST of CSPI04G24170.1 vs. NCBI nr
Match: KAG6591383.1 (Lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 612.8 bits (1579), Expect = 2.5e-171
Identity = 299/487 (61.40%), Postives = 366/487 (75.15%), Query Frame = 0


Query: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60
           MGS + + +E+ L LKP +A + DV  F+LP GSR+I+ L++CPD + E Y++FKAR TI
Sbjct: 3   MGSKQNEFSEDYLILKPKEASLVDVFLFILPLGSRRIRSLIDCPDAKEESYSSFKARCTI 62

Query: 61  SVSILTQKFLSAIASLFTILVTFWSIIQKFCYKYVLA-AEFWVAPSRIINFTDVQPRVRC 120
            VSIL QKFL A+A+    L  F S+IQ   + Y++   EF+         +D  P VRC
Sbjct: 63  FVSILVQKFLLALAAPLLTLRAFLSVIQPLFFNYMIGKGEFYTR-------SDSGPEVRC 122

Query: 121 SDWELLVPDDNADMNSQDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLID 180
            DW++  PD        D+ FKYY ALT+MAS+LAY+DY+ S S+V  VVN CWQM L+ 
Sbjct: 123 RDWQIEDPD--------DKGFKYYGALTMMASQLAYEDYTASPSVVHSVVNGCWQMTLLG 182

Query: 181 CRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVG 240
           C NFWNDFQNKATT A MF+NT KDPNVTV+AFRGTSV + +DWMVD + SW+ +EG   
Sbjct: 183 CFNFWNDFQNKATTGAFMFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWYKIEGIGR 242

Query: 241 IHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGAL 300
           IHSGFMQALG QK+ GWPKEL   +HEFAYY LRQ LR+I KSND AKFI+TGHSLGGAL
Sbjct: 243 IHSGFMQALGLQKATGWPKELPGAEHEFAYYTLRQRLRDIVKSNDKAKFIMTGHSLGGAL 302

Query: 301 ATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDL 360
           ATLFVTLLA H ++ +L ++QAVYTFGQPRVG++ FA+FMV+T   +  KYYRYVYSFDL
Sbjct: 303 ATLFVTLLALHQDSTILQRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGFKYYRYVYSFDL 362

Query: 361 VPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSADWEFIRS 420
           VPR+PF S+  F Y+HFGGC+YFD FYNGKF ++QPNTNYFSLIWVIPKYLSA WE I S
Sbjct: 363 VPRVPFDSII-FKYKHFGGCLYFDCFYNGKFRREQPNTNYFSLIWVIPKYLSAHWELISS 422

Query: 421 LIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWGNIHLPPDHHELLKF 480
           LII P+ KGR+YFEGF T +ER VGL  PG+SAH+C NYV++TRWG I + PD +E LK 
Sbjct: 423 LIIIPVFKGREYFEGFVTTLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDRNETLKL 473

Query: 481 AHYIEAD 487
             YIEAD
Sbjct: 483 VRYIEAD 473

[Close]

BLAST of CSPI04G24170.1 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 322.4 bits (825), Expect = 6.3e-88
Identity = 185/477 (38.78%), Postives = 286/477 (59.96%), Query Frame = 0


Query: 10  ENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIE-LYTNFKARWTISVSILTQK 69
           +N   L P +A V D++  LL S     +K +   +++IE   + F+ RW I VSI+ QK
Sbjct: 5   KNYFVLDPREATVSDLIH-LLFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQK 64

Query: 70  FLSAIASLFTILVTFWSIIQKFCYKYVLAAE--FWVAPSRIINFTDVQPRVRCSDWELLV 129
            +     LF   + F       C+  +L++   F +    +     + P    + +  L 
Sbjct: 65  LI----ILFKKPLYFVG-FSLACWLNLLSSNGGFLMILPNLFKGNIIWPEKASATFASLN 124

Query: 130 P--DDNADMNSQ-DRDFKYYSA-LTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDCRN 189
              D   ++N + +R  K Y A L+IMASKL+Y+    + + V  V+++ W+M L+   +
Sbjct: 125 GNLDRRVELNPKVERGSKRYKAMLSIMASKLSYE----NINFVSSVLHNHWKMDLLGFYS 184

Query: 190 FWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGIHS 249
            WN +Q + +T  I+ ++T  DPN+ +++FRGT   D +DW  DLD SW+ ++    IH 
Sbjct: 185 CWNGYQKQRSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIHG 244

Query: 250 GFMQALGYQKSGGWPKELTDPKHE-----FAYYFLRQNLREIAKSNDNAKFIITGHSLGG 309
           GFM+ALG QK  GWPKE+   + +     +AYY +R++L+EI   N  +KFI+TGHSLGG
Sbjct: 245 GFMKALGLQKE-GWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLGG 304

Query: 310 ALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSF 369
           ALA LF  +L  H+E  +L++++ VYTFGQPRVG++ F  FM D+ K  D+KY RYVY  
Sbjct: 305 ALAILFTAVLVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYCN 364

Query: 370 DLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSADWEFI 429
           D+VPR+PF       ++HFG C+Y+D FY GK  +++PN NYF+L+WV+PK ++A WE I
Sbjct: 365 DMVPRLPFDD-KTLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMNALWELI 424

Query: 430 RSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWGNIHLPPDHH 475
           RS ++ P  KG ++ EG+F    R V L+IPG+ AH  + Y+++T  G++   PD H
Sbjct: 425 RSFVM-PYWKGGEFREGWFLRCFRVVALLIPGLPAHFPNEYINVTLLGDL---PDSH 465

[Close]

BLAST of CSPI04G24170.1 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 318.2 bits (814), Expect = 1.2e-86
Identity = 180/479 (37.58%), Postives = 268/479 (55.95%), Query Frame = 0


Query: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60
           M    ++   +   + P KA   D+L  L  S     + +   PD       +F +RW +
Sbjct: 1   MSKTNMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWIL 60

Query: 61  SVSILTQKFLSAIASLFTIL---VTFWSIIQK-----FCYKYVLAAEFWVAPSRIINFTD 120
           +++I  QK L  ++  F  +   +T+W  +       F     L +   V P +  +   
Sbjct: 61  ALAIFLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKPDK--SSAT 120

Query: 121 VQPRVRCSDWELLVPDDNADMNSQDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDC 180
               + CSD  + + D+  ++ S     +Y S L+IMASK++Y+    S   +  VV + 
Sbjct: 121 YTSFIGCSDRRIEL-DEKINVGS----IEYKSMLSIMASKISYE----SKPYITSVVKNT 180

Query: 181 WQMKLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWF 240
           W+M L+   +F+N FQ    T A +F+ +  +P++ V++FRGT   +  DW  DLD SW+
Sbjct: 181 WKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWY 240

Query: 241 LLEGKVGIHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITG 300
            ++    +H+GF +ALG QK  GWPKE     H++AYY +RQ LR+    N N K+I+TG
Sbjct: 241 EMKNVGKVHAGFSRALGLQKD-GWPKENISLLHQYAYYTIRQMLRDKLGRNKNLKYILTG 300

Query: 301 HSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYR 360
           HSLGGALA LF  +LA H E  LLDK++ +YTFGQPRVG++ F +FM    K H I+Y R
Sbjct: 301 HSLGGALAALFPAILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYER 360

Query: 361 YVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYLSA 420
           +VY+ D+VPR+PF     FSY+H+G C  F+  Y GK  +  PN NYF+L+W+IP+ L+ 
Sbjct: 361 FVYNNDVVPRVPFDDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTG 420

Query: 421 DWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWGNIHLPP 472
            WEFIRS I+    KG +Y E +     R VG+V PG S H   +YV+ TR G +  PP
Sbjct: 421 LWEFIRSFIL-QFWKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRPP 466

[Close]

BLAST of CSPI04G24170.1 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 294.3 bits (752), Expect = 1.8e-79
Identity = 175/481 (36.38%), Postives = 258/481 (53.64%), Query Frame = 0


Query: 10  ENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTISVSILTQKF 69
           +N   L P +A V D++  L  S     K +    ++       F+ RW I VSI+ QK 
Sbjct: 5   KNYFVLDPREATVTDLMRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKL 64

Query: 70  LSAIASLFTIL---VTFWSIIQ-------KFCYKYVLAAEFWVAPSRIINFTDVQPRVRC 129
           L  +    + L   + FW  +        K     V     W   +    F  +   +  
Sbjct: 65  LIILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGRFIWPEKTS-ATFASINGNL-- 124

Query: 130 SDWELLVPDDNADM----NSQDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQM 189
                   D   ++    + +  D +Y   L+IMASKLAY+    +   +  V+ D WQM
Sbjct: 125 --------DQKVELGLGRSIKIGDERYKPLLSIMASKLAYE----NEHFIRSVLQDHWQM 184

Query: 190 KLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLE 249
            L+   +  NDF    +T  I+  +T  +PN+ V++FRGT   + +DW  DLD SW  + 
Sbjct: 185 DLLGFYSCPNDFDQTRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVM 244

Query: 250 GKVGIHSGFMQALGYQKSGGWPKELTDPK-----HEFAYYFLRQNLREIAKSNDNAKFII 309
               IH GFM+ALG  K  GW +E+   +      + AYY + + L+E+ + N  +KFI+
Sbjct: 245 NVGKIHGGFMKALGLPKE-GWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFIL 304

Query: 310 TGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKY 369
           +GHSLGGALA LF  +L  H+E  +L++++ VYTFGQPRVG++ F  +M D  K  D+KY
Sbjct: 305 SGHSLGGALAILFTAVLIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKY 364

Query: 370 YRYVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIPKYL 429
            RYVY  D+VPR+PF       ++HFGGC+Y D FY GK  +++PN NYF++ WVIPK +
Sbjct: 365 KRYVYCNDMVPRLPFDD-KTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKII 424

Query: 430 SADWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTRWGNIHLP 472
           +A WE IRS II+   +GR+Y EG+     R V L+IPG+ AH  + YV++   GN   P
Sbjct: 425 NAVWELIRSFIIS-CWQGREYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLGN--FP 465

[Close]

BLAST of CSPI04G24170.1 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 1.3e-77
Identity = 171/476 (35.92%), Postives = 266/476 (55.88%), Query Frame = 0


Query: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60
           M S++       L L+P++ +  +++  L    S  I+K       + E + +F+ RW I
Sbjct: 2   MNSDDDDEPRGYLILRPEELRPWELVRLLF---SGDIEKPRSVDSSETEEH-SFRHRWLI 61

Query: 61  SVSILTQKFLSAIASLFTILVTFWSIIQKFCYKYVLAAEFW----VAPSRIINFTDVQPR 120
            VS++  K L   + L  ++ +       F      +  F     V P R     + Q  
Sbjct: 62  FVSLVLLKLLRFFSKLLALVGSALEFSLNFLSNNSFSGLFLRGEVVMPQR--TSENYQSF 121

Query: 121 VRCSDWELLVPDDNADMNSQDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMK 180
           +   D  + +   +  +N +D + KYY+AL+IMASK+AY++ +R    ++ VV + W MK
Sbjct: 122 IGHLDTRVSL---DMTLNREDGE-KYYAALSIMASKIAYENAAR----IKHVVENHWNMK 181

Query: 181 LIDCRNFWNDFQNKATTHAIMFE------NTHKDPNVTVIAFRGTSVLDINDWMVDLDFS 240
            +   ++WN++Q K TT A +         ++      V+AFRGT + +  DW  D D +
Sbjct: 182 YLGLVDYWNEYQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDIT 241

Query: 241 WFLLEGKVGIHSGFMQALGYQKSGGWPKE-LTDP--KHEFAYYFLRQNLREIAKSNDNAK 300
           WF L     IH GFM+ALG Q +  WPKE L++P  K   AYY +R +L+ +   N N K
Sbjct: 242 WFELPNIGNIHGGFMKALGLQNNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTK 301

Query: 301 FIITGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHD 360
           F++TGHSLGGALA LF  +L  H+ET LL++IQ VYT+GQPRVG+  F +FM    + ++
Sbjct: 302 FVLTGHSLGGALAILFTAVLVIHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYN 361

Query: 361 IKYYRYVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKKQPNTNYFSLIWVIP 420
           IKYYR+VY+ D+VPR+P+    +  ++HFG C+Y+D  Y  K +++Q + N+F L  +I 
Sbjct: 362 IKYYRFVYNNDIVPRLPYDD-KDLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIK 421

Query: 421 KYLSADWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVSLTR 464
              SA  EFIRS  I    KG +Y EG+     R +G+++PG+S H   +YV+ TR
Sbjct: 422 MMWSAILEFIRSFTIV-AEKGSEYSEGWLLKGGRALGIIVPGVSNHTPQDYVNATR 461

[Close]

BLAST of CSPI04G24170.1 vs. TAIR 10
Match: AT1G45201.2 (triacylglycerol lipase-like 1 )

HSP 1 Score: 251.1 bits (640), Expect = 1.8e-66
Identity = 144/397 (36.27%), Postives = 219/397 (55.16%), Query Frame = 0


Query: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60
           M    ++   +   + P KA   D+L  L  S     + +   PD       +F +RW +
Sbjct: 1   MSKTNMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWIL 60

Query: 61  SVSILTQKFLSAIASLFTIL---VTFWSIIQK-----FCYKYVLAAEFWVAPSRIINFTD 120
           +++I  QK L  ++  F  +   +T+W  +       F     L +   V P +  +   
Sbjct: 61  ALAIFLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKPDK--SSAT 120

Query: 121 VQPRVRCSDWELLVPDDNADMNSQDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDC 180
               + CSD  + + D+  ++ S     +Y S L+IMASK++Y+    S   +  VV + 
Sbjct: 121 YTSFIGCSDRRIEL-DEKINVGS----IEYKSMLSIMASKISYE----SKPYITSVVKNT 180

Query: 181 WQMKLIDCRNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWF 240
           W+M L+   +F+N FQ    T A +F+ +  +P++ V++FRGT   +  DW  DLD SW+
Sbjct: 181 WKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWY 240

Query: 241 LLEGKVGIHSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITG 300
            ++    +H+GF +ALG QK  GWPKE     H++AYY +RQ LR+    N N K+I+TG
Sbjct: 241 EMKNVGKVHAGFSRALGLQKD-GWPKENISLLHQYAYYTIRQMLRDKLGRNKNLKYILTG 300

Query: 301 HSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYR 360
           HSLGGALA LF  +LA H E  LLDK++ +YTFGQPRVG++ F +FM    K H I+Y R
Sbjct: 301 HSLGGALAALFPAILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYER 360

Query: 361 YVYSFDLVPRIPFHSLANFSYRHFGGCVYFDVFYNGK 390
           +VY+ D+VPR+PF     FSY+H+G C  F+  Y GK
Sbjct: 361 FVYNNDVVPRVPFDDKYLFSYKHYGPCNSFNSLYKGK 385

The following BLAST results are available for this feature:

BLAST of CSPI04G24170.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JFU8	8.0e-64	38.46	Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1	[more]
A0A1S3ZP85	3.5e-59	34.04	Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1	[more]
Q5VKJ7	5.1e-58	32.41	Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1	[more]
O59952	8.7e-10	28.63	Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1	[more]
P19515	1.1e-09	34.50	Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2	[more]

BLAST of CSPI04G24170.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZS7	4.2e-289	99.18	Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639880 PE...	[more]
A0A6J1FDN5	9.3e-172	61.40	uncharacterized protein LOC111443149 OS=Cucurbita moschata OX=3662 GN=LOC1114431...	[more]
A0A6J1CEU2	1.3e-138	54.29	uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
A0A6J1C148	4.8e-128	51.69	uncharacterized protein LOC111007483 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1C321	6.9e-127	52.53	uncharacterized protein LOC111007483 isoform X1 OS=Momordica charantia OX=3673 G...	[more]

BLAST of CSPI04G24170.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031739748.1	8.6e-289	99.18	uncharacterized protein LOC105435389 [Cucumis sativus]	[more]
KGN55200.1	8.6e-289	99.18	hypothetical protein Csa_012841 [Cucumis sativus]	[more]
XP_038896694.1	4.1e-214	73.92	triacylglycerol lipase OBL1-like [Benincasa hispida]	[more]
XP_022936593.1	1.9e-171	61.40	uncharacterized protein LOC111443149 [Cucurbita moschata]	[more]
KAG6591383.1	2.5e-171	61.40	Lipase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CSPI04G24170.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42930.1	6.3e-88	38.78	alpha/beta-Hydrolases superfamily protein	[more]
AT1G45201.1	1.2e-86	37.58	triacylglycerol lipase-like 1	[more]
AT1G56630.1	1.8e-79	36.38	alpha/beta-Hydrolases superfamily protein	[more]
AT5G67050.1	1.3e-77	35.92	alpha/beta-Hydrolases superfamily protein	[more]
AT1G45201.2	1.8e-66	36.27	triacylglycerol lipase-like 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 129..403 e-value: 5.3E-47 score: 162.2
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 142..392
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 209..366 e-value: 7.3E-35 score: 119.9
None	No IPR available	PANTHER	PTHR46086:SF4	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 6..470
None	No IPR available	CDD	cd00519	Lipase_3	coord: 140..383 e-value: 1.21209E-40 score: 143.771
IPR044819	Triacylglycerol lipase OBL1-like	PANTHER	PTHR46086	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 6..470

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI04G24170	CSPI04G24170	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G24170.1.cds4	CSPI04G24170.1.cds4	CDS
CSPI04G24170.1.cds3	CSPI04G24170.1.cds3	CDS
CSPI04G24170.1.cds2	CSPI04G24170.1.cds2	CDS
CSPI04G24170.1.cds1	CSPI04G24170.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI04G24170.1	CSPI04G24170.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004806	triglyceride lipase activity