CSPI04G23520.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI04G23520.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	translation initiation factor IF-1, chloroplastic-like
Location	Chr4: 21596258 .. 21597044 (+)
Sequence length	787
RNA-Seq Expression	CSPI04G23520.1
Synteny	CSPI04G23520.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAAAAAAGCGTAGGCCTATCCCCTTCTCCATCGTAGTTTCTTTGGCGCTCCCTCAGTCAGCCAAAATCTTTGAAGACTGAATTTGGAGCAAAAATGTCGAGCACAAATCTCCTACAGTTCCCTCTCCTTCAATCCCACTTCCGCCCACCGTCCACTCTCCTTTTTCCCGCCGTCAAATCCCATCTCTCCCTCGCCTCAATCTCCTTTCCCACCTCCCAATTCCTTCATACCCCTTCTCATGCTTCTCCTGCCTTCGCTCTCATGGCCAAGTCCCCCAAAAACGACACCTCCGAGCAGAAATTGAGCCATGAAGGCTCCGTCGTCGAGTCTCTTCCCAATGGGATGTTCAGGGTTCGGTTGGATAATCAAGACCTCATTCTAGGCTACATATCCGGTAAGATTCGGAAGAATTTTGTTCGTATTTTGCCTGGTGATAGGGTTAGGGTTGAGGTTAGTCGTTATGATTCTACCAAAGGTCGAATTGTTTATAGAATGCGTAGTACTAAAGATTCATCTTCATGAATACTAGTTTTGGATTATCATTATTATTATTATTTGCCTTTAATGTGTTGATTTTGTGACATATTGTGTACATTGATCCCTCTTTTTTTGGCCTTTTTTAGAATGTAAAATGACAAACATTTAGTTTATTTGAGCTCCTTTGTTTTAGTGATTTGTATTTGTGGATGAATCACTTGGAGTATTAGAGCTTGTGAATTTTTGTCTTGTCTTGTAATTGTTTTATACTAAAGAAAGAGGAGTTTCTAAATGATATTGATTTTTCA

mRNA sequence

GAAAAAAAAGCGTAGGCCTATCCCCTTCTCCATCGTAGTTTCTTTGGCGCTCCCTCAGTCAGCCAAAATCTTTGAAGACTGAATTTGGAGCAAAAATGTCGAGCACAAATCTCCTACAGTTCCCTCTCCTTCAATCCCACTTCCGCCCACCGTCCACTCTCCTTTTTCCCGCCGTCAAATCCCATCTCTCCCTCGCCTCAATCTCCTTTCCCACCTCCCAATTCCTTCATACCCCTTCTCATGCTTCTCCTGCCTTCGCTCTCATGGCCAAGTCCCCCAAAAACGACACCTCCGAGCAGAAATTGAGCCATGAAGGCTCCGTCGTCGAGTCTCTTCCCAATGGGATGTTCAGGGTTCGGTTGGATAATCAAGACCTCATTCTAGGCTACATATCCGGTAAGATTCGGAAGAATTTTGTTCGTATTTTGCCTGGTGATAGGGTTAGGGTTGAGGTTAGTCGTTATGATTCTACCAAAGGTCGAATTGTTTATAGAATGCGTAGTACTAAAGATTCATCTTCATGAATACTAGTTTTGGATTATCATTATTATTATTATTTGCCTTTAATGTGTTGATTTTGTGACATATTGTGTACATTGATCCCTCTTTTTTTGGCCTTTTTTAGAATGTAAAATGACAAACATTTAGTTTATTTGAGCTCCTTTGTTTTAGTGATTTGTATTTGTGGATGAATCACTTGGAGTATTAGAGCTTGTGAATTTTTGTCTTGTCTTGTAATTGTTTTATACTAAAGAAAGAGGAGTTTCTAAATGATATTGATTTTTCA

Coding sequence (CDS)

ATGTCGAGCACAAATCTCCTACAGTTCCCTCTCCTTCAATCCCACTTCCGCCCACCGTCCACTCTCCTTTTTCCCGCCGTCAAATCCCATCTCTCCCTCGCCTCAATCTCCTTTCCCACCTCCCAATTCCTTCATACCCCTTCTCATGCTTCTCCTGCCTTCGCTCTCATGGCCAAGTCCCCCAAAAACGACACCTCCGAGCAGAAATTGAGCCATGAAGGCTCCGTCGTCGAGTCTCTTCCCAATGGGATGTTCAGGGTTCGGTTGGATAATCAAGACCTCATTCTAGGCTACATATCCGGTAAGATTCGGAAGAATTTTGTTCGTATTTTGCCTGGTGATAGGGTTAGGGTTGAGGTTAGTCGTTATGATTCTACCAAAGGTCGAATTGTTTATAGAATGCGTAGTACTAAAGATTCATCTTCATGA

Protein sequence

MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAFALMAKSPKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEVSRYDSTKGRIVYRMRSTKDSSS*

Homology

[Close]

BLAST of CSPI04G23520.1 vs. ExPASy Swiss-Prot
Match: Q94KR7 (Translation initiation factor IF-1, chloroplastic OS=Glycine max OX=3847 GN=infA PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.4e-29
Identity = 67/103 (65.05%), Postives = 82/103 (79.61%), Query Frame = 0


Query: 40  TSQFLHTPS--HASPAFALMAKSPKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILG 99
           T  FL  P    A+PA +  + +  + + EQK  HEG ++ESLPNGMFRVRLDN+DLILG
Sbjct: 36  TLSFLAPPPLLPAAPALSAASAAKPDKSGEQKWVHEGLIMESLPNGMFRVRLDNEDLILG 95

Query: 100 YISGKIRKNFVRILPGDRVRVEVSRYDSTKGRIVYRMRSTKDS 141
           YISGKIRKN+VRILPGDRV+VEV+RYDS+KGRIVYR+RS+  S
Sbjct: 96  YISGKIRKNYVRILPGDRVKVEVTRYDSSKGRIVYRLRSSTPS 138

[Close]

BLAST of CSPI04G23520.1 vs. ExPASy Swiss-Prot
Match: A6MMP2 (Translation initiation factor IF-1, chloroplastic OS=Dioscorea elephantipes OX=145284 GN=infA PE=3 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 2.7e-28
Identity = 58/75 (77.33%), Postives = 72/75 (96.00%), Query Frame = 0


Query: 67  EQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEVSRYDST 126
           EQKL HEG ++ESLPNGMFRVRLDN+DLILGY+SG+IR++F+RILPGDRV++EVSRYDST
Sbjct: 3   EQKLIHEGVIIESLPNGMFRVRLDNEDLILGYVSGRIRRSFIRILPGDRVKIEVSRYDST 62

Query: 127 KGRIVYRMRSTKDSS 142
           KGRI+YR+R+ KDS+
Sbjct: 63  KGRIIYRLRN-KDSN 76

[Close]

BLAST of CSPI04G23520.1 vs. ExPASy Swiss-Prot
Match: Q95GN5 (Translation initiation factor IF-1, chloroplastic OS=Asarum canadense OX=28498 GN=infA PE=3 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 6.1e-28
Identity = 57/75 (76.00%), Postives = 72/75 (96.00%), Query Frame = 0


Query: 67  EQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEVSRYDST 126
           EQKL HEG ++ESLPNGMFRVRLDN+DLILGY+SG+IR++F+RILPGDRV++EVSRYDST
Sbjct: 3   EQKLIHEGLIIESLPNGMFRVRLDNEDLILGYVSGRIRRSFIRILPGDRVKIEVSRYDST 62

Query: 127 KGRIVYRMRSTKDSS 142
           +GRI+YR+R+ KDS+
Sbjct: 63  RGRIIYRLRN-KDSN 76

[Close]

BLAST of CSPI04G23520.1 vs. ExPASy Swiss-Prot
Match: A6MMF7 (Translation initiation factor IF-1, chloroplastic OS=Chloranthus spicatus OX=13006 GN=infA PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.4e-27
Identity = 57/75 (76.00%), Postives = 71/75 (94.67%), Query Frame = 0


Query: 67  EQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEVSRYDST 126
           EQKL HEG + ESLPNGMFRVRLDN+DLILGY+SG+IR++F+RILPGDRV++EVSRYDST
Sbjct: 3   EQKLIHEGLITESLPNGMFRVRLDNEDLILGYVSGRIRRSFIRILPGDRVKIEVSRYDST 62

Query: 127 KGRIVYRMRSTKDSS 142
           +GRI+YR+R+ KDS+
Sbjct: 63  RGRIIYRLRN-KDSN 76

[Close]

BLAST of CSPI04G23520.1 vs. ExPASy Swiss-Prot
Match: A0A370 (Translation initiation factor IF-1, chloroplastic OS=Coffea arabica OX=13443 GN=infA PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 1.4e-27
Identity = 57/74 (77.03%), Postives = 70/74 (94.59%), Query Frame = 0


Query: 67  EQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEVSRYDST 126
           EQK +HEG + ESLPNGMFRVRLDN+DLILGY+SGKIR++F+RILPGDRV++EVSRYDST
Sbjct: 3   EQKWTHEGLITESLPNGMFRVRLDNEDLILGYVSGKIRRSFIRILPGDRVKIEVSRYDST 62

Query: 127 KGRIVYRMRSTKDS 141
           +GRI+YR+R+ KDS
Sbjct: 63  RGRIIYRLRN-KDS 75

[Close]

BLAST of CSPI04G23520.1 vs. ExPASy TrEMBL
Match: A0A0A0L4Q7 (S1-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G637710 PE=3 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.8e-70
Identity = 141/142 (99.30%), Postives = 141/142 (99.30%), Query Frame = 0


Query: 1   MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAFALMAKS 60
           MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAF LMAKS
Sbjct: 1   MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAFVLMAKS 60

Query: 61  PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEV 120
           PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEV
Sbjct: 61  PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEV 120

Query: 121 SRYDSTKGRIVYRMRSTKDSSS 143
           SRYDSTKGRIVYRMRSTKDSSS
Sbjct: 121 SRYDSTKGRIVYRMRSTKDSSS 142

[Close]

BLAST of CSPI04G23520.1 vs. ExPASy TrEMBL
Match: A0A5A7V7J3 (Translation initiation factor IF-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003940 PE=3 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 5.8e-66
Identity = 134/143 (93.71%), Postives = 138/143 (96.50%), Query Frame = 0


Query: 1   MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAFALMAKS 60
           MSSTNLLQFPLLQSHFRPPSTLLFP VKSHLS+ SISFPTSQFLHTPSHA P FALMAKS
Sbjct: 1   MSSTNLLQFPLLQSHFRPPSTLLFPPVKSHLSVVSISFPTSQFLHTPSHAPPGFALMAKS 60

Query: 61  PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEV 120
           PKNDTSEQKLSHEGSVVESLPNGMFRVRLDN+DLILGYISGKIRKNFVRILPGDRVRVEV
Sbjct: 61  PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNEDLILGYISGKIRKNFVRILPGDRVRVEV 120

Query: 121 SRYDSTKGRIVYRMRST-KDSSS 143
           SRYDSTKGRIVYR+RS+ KDSSS
Sbjct: 121 SRYDSTKGRIVYRLRSSGKDSSS 143

[Close]

BLAST of CSPI04G23520.1 vs. ExPASy TrEMBL
Match: A0A6J1FLU6 (translation initiation factor IF-1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111446393 PE=3 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 4.9e-57
Identity = 116/142 (81.69%), Postives = 127/142 (89.44%), Query Frame = 0


Query: 1   MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAFALMAKS 60
           MSSTNLLQFPLLQ   RPPST LFPA+KSH+S+ SI FP+SQFLH PSH   +F  MAKS
Sbjct: 1   MSSTNLLQFPLLQFQCRPPSTFLFPAIKSHVSVVSIPFPSSQFLHNPSHGPVSFVPMAKS 60

Query: 61  PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEV 120
           PK+++SEQK SHEGSV+ESLPNGMFRV+LDN+DLILGYISGKIRKNFVRILPGDRVRVEV
Sbjct: 61  PKSESSEQKFSHEGSVIESLPNGMFRVQLDNEDLILGYISGKIRKNFVRILPGDRVRVEV 120

Query: 121 SRYDSTKGRIVYRMRSTKDSSS 143
           SRYDSTKGRIVYR RSTKD SS
Sbjct: 121 SRYDSTKGRIVYRHRSTKDPSS 142

[Close]

BLAST of CSPI04G23520.1 vs. ExPASy TrEMBL
Match: A0A6J1IW62 (translation initiation factor IF-1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111480474 PE=3 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 3.2e-56
Identity = 116/142 (81.69%), Postives = 125/142 (88.03%), Query Frame = 0


Query: 1   MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAFALMAKS 60
           MSSTNLLQFPLLQ   RPP T LFP  KSHLS+ SI FP+SQFLH PSH   +FA MAKS
Sbjct: 1   MSSTNLLQFPLLQFQCRPPYTSLFPTTKSHLSVVSIPFPSSQFLHNPSHGPASFAPMAKS 60

Query: 61  PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEV 120
           PK+++SEQK SHEGSV+ESLPNGMFRV+LDN+DLILGYISGKIRKNFVRILPGDRVRVEV
Sbjct: 61  PKSESSEQKFSHEGSVIESLPNGMFRVQLDNEDLILGYISGKIRKNFVRILPGDRVRVEV 120

Query: 121 SRYDSTKGRIVYRMRSTKDSSS 143
           SRYDSTKGRIVYR RSTKD SS
Sbjct: 121 SRYDSTKGRIVYRHRSTKDPSS 142

[Close]

BLAST of CSPI04G23520.1 vs. ExPASy TrEMBL
Match: A0A6J1IP00 (uncharacterized protein LOC111476947 OS=Cucurbita maxima OX=3661 GN=LOC111476947 PE=3 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 2.3e-54
Identity = 111/137 (81.02%), Postives = 122/137 (89.05%), Query Frame = 0


Query: 1   MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAFALMAKS 60
           MSSTNLL+FPLL  H RPPSTL FPA+KSHLS+ SI+FPTSQFL  PSH    FALMAK 
Sbjct: 1   MSSTNLLRFPLLHLHSRPPSTLFFPAIKSHLSVVSIAFPTSQFLRNPSHGPARFALMAKP 60

Query: 61  PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEV 120
           PK+D++EQK S+EGS+VESLPNGMFRVRLDN+DLILGYI GKIRKNFVRILPGDRVRVEV
Sbjct: 61  PKSDSTEQKWSYEGSIVESLPNGMFRVRLDNEDLILGYICGKIRKNFVRILPGDRVRVEV 120

Query: 121 SRYDSTKGRIVYRMRST 138
           SRYDSTKGRIVYR RS+
Sbjct: 121 SRYDSTKGRIVYRNRSS 137

[Close]

BLAST of CSPI04G23520.1 vs. NCBI nr
Match: XP_011654076.1 (uncharacterized protein LOC101206284 [Cucumis sativus] >KGN55131.1 hypothetical protein Csa_012104 [Cucumis sativus])

HSP 1 Score: 275.0 bits (702), Expect = 3.6e-70
Identity = 141/142 (99.30%), Postives = 141/142 (99.30%), Query Frame = 0


Query: 1   MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAFALMAKS 60
           MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAF LMAKS
Sbjct: 1   MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAFVLMAKS 60

Query: 61  PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEV 120
           PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEV
Sbjct: 61  PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEV 120

Query: 121 SRYDSTKGRIVYRMRSTKDSSS 143
           SRYDSTKGRIVYRMRSTKDSSS
Sbjct: 121 SRYDSTKGRIVYRMRSTKDSSS 142

[Close]

BLAST of CSPI04G23520.1 vs. NCBI nr
Match: KAA0064332.1 (translation initiation factor IF-1 [Cucumis melo var. makuwa] >TYK20254.1 translation initiation factor IF-1 [Cucumis melo var. makuwa])

HSP 1 Score: 260.0 bits (663), Expect = 1.2e-65
Identity = 134/143 (93.71%), Postives = 138/143 (96.50%), Query Frame = 0


Query: 1   MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAFALMAKS 60
           MSSTNLLQFPLLQSHFRPPSTLLFP VKSHLS+ SISFPTSQFLHTPSHA P FALMAKS
Sbjct: 1   MSSTNLLQFPLLQSHFRPPSTLLFPPVKSHLSVVSISFPTSQFLHTPSHAPPGFALMAKS 60

Query: 61  PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEV 120
           PKNDTSEQKLSHEGSVVESLPNGMFRVRLDN+DLILGYISGKIRKNFVRILPGDRVRVEV
Sbjct: 61  PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNEDLILGYISGKIRKNFVRILPGDRVRVEV 120

Query: 121 SRYDSTKGRIVYRMRST-KDSSS 143
           SRYDSTKGRIVYR+RS+ KDSSS
Sbjct: 121 SRYDSTKGRIVYRLRSSGKDSSS 143

[Close]

BLAST of CSPI04G23520.1 vs. NCBI nr
Match: XP_038899010.1 (translation initiation factor IF-1, chloroplastic [Benincasa hispida])

HSP 1 Score: 249.2 bits (635), Expect = 2.1e-62
Identity = 129/143 (90.21%), Postives = 134/143 (93.71%), Query Frame = 0


Query: 1   MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAFALMAKS 60
           MSST LLQFPLLQSHFRPPSTL FPAVKSHLS+ SI+FPT+QFLH PSHAS  F LMAKS
Sbjct: 1   MSSTILLQFPLLQSHFRPPSTLFFPAVKSHLSVVSITFPTTQFLHNPSHASAGFVLMAKS 60

Query: 61  PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEV 120
           PKNDTSEQK SHEGSVVESLPNGMFRVRLDN+DLILGYISGKIRKNFVRILPGDRVRVEV
Sbjct: 61  PKNDTSEQKWSHEGSVVESLPNGMFRVRLDNEDLILGYISGKIRKNFVRILPGDRVRVEV 120

Query: 121 SRYDSTKGRIVYRMR-STKDSSS 143
           SRYDSTKGRIVYR+R STKDSSS
Sbjct: 121 SRYDSTKGRIVYRLRSSTKDSSS 143

[Close]

BLAST of CSPI04G23520.1 vs. NCBI nr
Match: KAG6607876.1 (Translation initiation factor IF-1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 233.8 bits (595), Expect = 9.2e-58
Identity = 118/142 (83.10%), Postives = 128/142 (90.14%), Query Frame = 0


Query: 1   MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAFALMAKS 60
           MSSTNLLQFPLLQ   RPPST LFPA+KSHLS+ SI FP+SQFLH PSH S +F  MAKS
Sbjct: 1   MSSTNLLQFPLLQFQCRPPSTFLFPAIKSHLSVVSIPFPSSQFLHNPSHGSASFVPMAKS 60

Query: 61  PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEV 120
           PK+++SEQK SHEGSV+ESLPNGMFRV+LDN+DLILGYISGKIRKNFVRILPGDRVRVEV
Sbjct: 61  PKSESSEQKFSHEGSVIESLPNGMFRVQLDNEDLILGYISGKIRKNFVRILPGDRVRVEV 120

Query: 121 SRYDSTKGRIVYRMRSTKDSSS 143
           SRYDSTKGRIVYR RSTKD SS
Sbjct: 121 SRYDSTKGRIVYRHRSTKDPSS 142

[Close]

BLAST of CSPI04G23520.1 vs. NCBI nr
Match: XP_022940969.1 (translation initiation factor IF-1, chloroplastic-like [Cucurbita moschata] >XP_023525132.1 translation initiation factor IF-1, chloroplastic-like [Cucurbita pepo subsp. pepo] >KAG7037405.1 Translation initiation factor IF-1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 230.3 bits (586), Expect = 1.0e-56
Identity = 116/142 (81.69%), Postives = 127/142 (89.44%), Query Frame = 0


Query: 1   MSSTNLLQFPLLQSHFRPPSTLLFPAVKSHLSLASISFPTSQFLHTPSHASPAFALMAKS 60
           MSSTNLLQFPLLQ   RPPST LFPA+KSH+S+ SI FP+SQFLH PSH   +F  MAKS
Sbjct: 1   MSSTNLLQFPLLQFQCRPPSTFLFPAIKSHVSVVSIPFPSSQFLHNPSHGPVSFVPMAKS 60

Query: 61  PKNDTSEQKLSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEV 120
           PK+++SEQK SHEGSV+ESLPNGMFRV+LDN+DLILGYISGKIRKNFVRILPGDRVRVEV
Sbjct: 61  PKSESSEQKFSHEGSVIESLPNGMFRVQLDNEDLILGYISGKIRKNFVRILPGDRVRVEV 120

Query: 121 SRYDSTKGRIVYRMRSTKDSSS 143
           SRYDSTKGRIVYR RSTKD SS
Sbjct: 121 SRYDSTKGRIVYRHRSTKDPSS 142

[Close]

BLAST of CSPI04G23520.1 vs. TAIR 10
Match: AT4G11175.1 (Nucleic acid-binding, OB-fold-like protein )

HSP 1 Score: 100.1 bits (248), Expect = 1.5e-21
Identity = 60/127 (47.24%), Postives = 75/127 (59.06%), Query Frame = 0


Query: 11  LLQSHFRPPSTLLFPAVKSHLSLASISF-PTSQFLHTPSHASPAFALMAKSPKNDTSEQK 70
           LL   FR  + +L P +    S + ++  P  +          A       P+      K
Sbjct: 13  LLPCQFRFTNGVLIPQINYVASNSVVNIRPMIRCQRASGGRGGANRSKPAKPQVKEGSNK 72

Query: 71  LSHEGSVVESLPNGMFRVRLDNQDLILGYISGKIRKNFVRILPGDRVRVEVSRYDSTKGR 130
              EG V ESLPNGMFRV L+N D ILGYI GKIRKNF+RILPGD+V+VE+S YDSTKGR
Sbjct: 73  TVIEGLVTESLPNGMFRVDLENGDNILGYICGKIRKNFIRILPGDKVKVEMSVYDSTKGR 132

Query: 131 IVYRMRS 137
           I++RM S
Sbjct: 133 IIFRMSS 139

The following BLAST results are available for this feature:

BLAST of CSPI04G23520.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94KR7	1.4e-29	65.05	Translation initiation factor IF-1, chloroplastic OS=Glycine max OX=3847 GN=infA...	[more]
A6MMP2	2.7e-28	77.33	Translation initiation factor IF-1, chloroplastic OS=Dioscorea elephantipes OX=1...	[more]
Q95GN5	6.1e-28	76.00	Translation initiation factor IF-1, chloroplastic OS=Asarum canadense OX=28498 G...	[more]
A6MMF7	1.4e-27	76.00	Translation initiation factor IF-1, chloroplastic OS=Chloranthus spicatus OX=130...	[more]
A0A370	1.4e-27	77.03	Translation initiation factor IF-1, chloroplastic OS=Coffea arabica OX=13443 GN=...	[more]

BLAST of CSPI04G23520.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L4Q7	1.8e-70	99.30	S1-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G637710 PE=...	[more]
A0A5A7V7J3	5.8e-66	93.71	Translation initiation factor IF-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1FLU6	4.9e-57	81.69	translation initiation factor IF-1, chloroplastic-like OS=Cucurbita moschata OX=...	[more]
A0A6J1IW62	3.2e-56	81.69	translation initiation factor IF-1, chloroplastic-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1IP00	2.3e-54	81.02	uncharacterized protein LOC111476947 OS=Cucurbita maxima OX=3661 GN=LOC111476947...	[more]

BLAST of CSPI04G23520.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011654076.1	3.6e-70	99.30	uncharacterized protein LOC101206284 [Cucumis sativus] >KGN55131.1 hypothetical ...	[more]
KAA0064332.1	1.2e-65	93.71	translation initiation factor IF-1 [Cucumis melo var. makuwa] >TYK20254.1 transl...	[more]
XP_038899010.1	2.1e-62	90.21	translation initiation factor IF-1, chloroplastic [Benincasa hispida]	[more]
KAG6607876.1	9.2e-58	83.10	Translation initiation factor IF-1, chloroplastic, partial [Cucurbita argyrosper...	[more]
XP_022940969.1	1.0e-56	81.69	translation initiation factor IF-1, chloroplastic-like [Cucurbita moschata] >XP_...	[more]

BLAST of CSPI04G23520.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G11175.1	1.5e-21	47.24	Nucleic acid-binding, OB-fold-like protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022967	RNA-binding domain, S1	SMART	SM00316	S1_6	coord: 67..135 e-value: 0.0044 score: 26.3
IPR006196	RNA-binding domain, S1, IF1 type	PFAM	PF01176	eIF-1a	coord: 73..133 e-value: 5.6E-22 score: 77.3
IPR006196	RNA-binding domain, S1, IF1 type	PROSITE	PS50832	S1_IF1_TYPE	coord: 61..135 score: 20.225674
None	No IPR available	GENE3D	2.40.50.140		coord: 61..142 e-value: 5.0E-33 score: 114.5
None	No IPR available	PANTHER	PTHR33370:SF1	TRANSLATION INITIATION FACTOR IF-1, CHLOROPLASTIC	coord: 31..139
IPR004368	Translation initiation factor IF-1	TIGRFAM	TIGR00008	TIGR00008	coord: 67..134 e-value: 2.2E-34 score: 115.3
IPR004368	Translation initiation factor IF-1	PANTHER	PTHR33370	TRANSLATION INITIATION FACTOR IF-1, CHLOROPLASTIC	coord: 31..139
IPR004368	Translation initiation factor IF-1	HAMAP	MF_00075	IF_1	coord: 57..135 score: 23.327375
IPR004368	Translation initiation factor IF-1	CDD	cd04451	S1_IF1	coord: 73..133 e-value: 3.0513E-34 score: 112.229
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 67..133

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI04G23520	CSPI04G23520	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G23520.1.utr5p1	CSPI04G23520.1.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G23520.1.cds1	CSPI04G23520.1.cds1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G23520.1.utr3p1	CSPI04G23520.1.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI04G23520.1	CSPI04G23520.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006413	translational initiation
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005829	cytosol
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0003743	translation initiation factor activity
molecular_function	GO:0043022	ribosome binding