CSPI04G18270.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI04G18270.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Serine hydroxymethyltransferase
Location	Chr4: 15657346 .. 15660877 (+)
Sequence length	2500
RNA-Seq Expression	CSPI04G18270.1
Synteny	CSPI04G18270.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGCTATTCCCCTCATCCGGTTAACACTACCTCCCTCTTCCTTCCTCTTCCTTCCCCCTCCCCCTCCCATCTTTGCATCCACATTCATCGCCCTTCCTTCACTGCCATTTCTTTCTTATTCTCTTCTCCATTTTAAACTGCATTTCCTTCATTTTTATTATAAATTCCATTTTCATTCTCCTTTTCCATCTTCCAGTCCTTACATGGAACTTTCTTCTTCCTCTACAACCCCTCGCCGCTCCTTCTTCCGTTACTGTCACTGTACTTTCTTCCGCCCTTGTTGTCGCTGTTTCTGTTGCTTCTACTCCTATCCCTAATTTCCCATCCCATTCCTTTTCTTCTTCTTCTTTTTCGTCTTCTTCTTCGTCGTCTTCTTCTTCTTCGTCTTCTTCATCTTCTTCATTATTATATTCTGCGTTGTTAGGGTTTATGGATTTTCGCCACGCCCCCTCGGATCTTTCCTTCCGCTTAAGTTCTCATACCACATCTTCCATATCTAGAAGTTCAACTGGGGACAATTCCAGCTATTTGTGTTCCACGGGTTCCAATTCCTCGTACCCGTCGGATTTCCCTCAGCATAAGGATGAGAAATTCGATTTGGTGAAGGGGGTTGCGGATGATGATAGCGACGACGAGAAGTTTAGTCTTCTAGGGCAACCCTTGCGTGTGAAGAGACAGAGGGACGCTCATTCATTTCTTAATCAGGATTCTGCGAAACGGGCTGCAGTCACAGATGAATCGAGCCTTGACATGAGACGAGTTTTGGTAAAGGCATGGGGGAATCAGTCGCTTCGAATTGCTGACCCGGAAATTCATAATATTATGGTGAAGGAGAAGGAGAGACAATATAAGGGTATTGAATTAGTTGCTTCGGAAAATTTTGTTTGTAGGGCTGTGATGGAAGCCTTAGGCAGCCATTTAACGAATAAGTATTCTGAGGGAATGCCTGGCGCTAGATACTATACGGGTAATCAGTATATAGATGAGATTGAATTGCTCTGTTGTTCGAGAGCCTTGGCTGCTTTTGACCTCGATTCTGAGAAATGGGGTGTGAATGTCCAACCTTACTCTTGTACATCAGCAAATTTTGCTGTGTATACAGGTCTTTTGTCTCCGAAGGATCGAATTATGGGTTTAGATTCAGCATCCGGAGGGCATCTTAGTCATGGGTATTATTCTCCTGTTGGGAAAAAAATTTCAGCGACTTCTATTTTCTTTGAGAGTCTTCCTTATAGAGTGAACCCCCTAACTGGATATGTTGATTATGATAAGCTTGAAGAAAAGGCGCTCGATTATCGTCCGAAAATACTCATCTGTGGAGGAAGTTCGTATCCACGCGAGTGGGATTATGCTAGGTGTAGACAGATCGCGGACAAATGCGGGGCGGTTTTGATGTGTGATATGGCCCATATAAGCGGTTTGGTGGCAGCCAAGGTGTGTAAATTTATTTGACGTTACAGTCCTTGGGACTTGTGATTGAATTCTTTTAGTCAGATATATGATTCATGATTCATGATTTATGATAAAGCTTGATTAAGGTAATATGCGCTTGTCCCGGGGTGTAATCATTTTAGTCTGTGAGATTGCAATTTGAACTTCCGTCCCATTCTTGATAATTCATGTATCATCATATCATATTAGCGATCTAAAAGTTAGTCCCACGTATGCCACTCGTTTATTTATGACTTTGTATTTACTGTAGGAATGTGCTAGTCCGTTTGAATATTGTGATATAGTTACTTCAACAACACACAAAAGTCTTCGAGGGCCCAGAGGAGGAATCATATTTTTCAGGAGGGGTCTAAAATCAAGGAAGCAAGGCGTGCTTCTAGCTCGTGGAGATGACACTGGTACTGCTACTTATGATTTTGAGGACAGGATAAACTTTTCTGTTTTCCCGTCGTTACAAGGGGGTCCCCATAATAATCATATTGCTGCCCTGGCCATAGCGTTAAAGCAAGTAGCTTCTCCCGAGTATAGAGTATATATTCGACAGGTGAAGAAAAATGCGCAGGCTTTAGCATCAGCTTTACTAAGAAGAAGTTGCAGATTGGTAACTAATGGTACTGACAATCATTTGGTGCTGTGGGATCTTACTCCTTTGGGACTAACAGGTACTATACTTGGATATTTGCCTCCTCACGGAGACACACTGTTCAATTGCACTGTAGGTTTGGTTTGAATGCTTCAAATTTTGTTGTTAGCTCTTCTAGTACTATTGTGATTTGATTTTGTATATGCTGATATGGTTTTCTTATGCGATAAGACCCTTGAGCAAATATCATTAGGTACTAACTTTTGGTGCTTAGTTTTGCGCTTTCCATTAATCATTTTTGCTGGACTATCAAGTTAACTTGTTCGGTTTGTCTCTGGTTTTGATTTGATTGTCTTATCCTGTCACTATTAGATAAGATTGAAGGAATTAAATCATAGTTCCCCCTTTCCCTGTTGTGTAACTTGGCTTCTCATGCTAAAGTCATAGGTTGATTGCATCCATCTGTGAGCATGTTGTTGAAAAAGAAGTTTCTAGACTTGTTTTCTCTTTAATATTCAGATTCTTTAAACACTTATTTCAATGTTTACATAAGTTTGTACAATATGGAGGAAAGTAACAACAGTGTCAAGACAACACCAAATTCATGTGAATGTTATTGGGTAAAGTTGTCCCTTGTGGGTTGTCAAGAAAAGAGCATCTAAGGTTGTAATGACAACTAATTTCTGATACTTTGATGTTTAATCTTATGCTGCAGCCAAGAATTATGAGATGATCTGTGAGGCATGTCATATTACAGTCAACAAGAGTGCCATTTATGGTGACAATGGAGCCATCTCCCCTAGAGGAGTAAGAATAGGTATGCAACTTTGTCTTATTTAGTACTTACTCAGCTTTGTAATCCTCATAATTCTGATTAGTGATTGCTGAGAGAGAATTTATAATGAAGTTGCGACTTATAGGAAACCAACTTGTTTCCTTGTTCAGGCACTCCTGCAATGACGACTAGAGGCTGTCTAGAGGCTGATTTTGAGACGATAGCTGAGATTCTTCTTAAAGCAGCTCATATCACAAAAATAGTCGTTCGTAGAGGAAAGCTTGGAAAATTACATAAAGGTATCATGAAGAATCTACAAAACAACAAAGATATTGTGGAGCTTCGGAACCAGGTCGAGGCCTTTGCTGCTTCATTTGCAATGCCAGGATTTGATATATGAATATATGAGCTTCTTGCTTGCTGCTATATACAACCACTTCATTTGGTAACACTAAATATGAATTCGTGTGCTTGTAAATTATAGGAAACTACTTAGTACGTAATACTGCACAAAAAGGTCCAAGTTTGACATGTATCATTCCTTTTATTGTAACAAGTGAAATCTCAGTTTATGACAGAGAAATTTTCAAGTCAAATATCTCCCATTTAGACGGTGGTTTTGTGTTGTGGGGTGTGTGTCTGTGGATAATTCAAAACCTCACATGAACTGATTATTCTGTCGTTTGTTTGCTGCAATATATACAATAGACTGTTTTGTCGCTG

mRNA sequence

GGCTATTCCCCTCATCCGGTTAACACTACCTCCCTCTTCCTTCCTCTTCCTTCCCCCTCCCCCTCCCATCTTTGCATCCACATTCATCGCCCTTCCTTCACTGCCATTTCTTTCTTATTCTCTTCTCCATTTTAAACTGCATTTCCTTCATTTTTATTATAAATTCCATTTTCATTCTCCTTTTCCATCTTCCAGTCCTTACATGGAACTTTCTTCTTCCTCTACAACCCCTCGCCGCTCCTTCTTCCGTTACTGTCACTGTACTTTCTTCCGCCCTTGTTGTCGCTGTTTCTGTTGCTTCTACTCCTATCCCTAATTTCCCATCCCATTCCTTTTCTTCTTCTTCTTTTTCGTCTTCTTCTTCGTCGTCTTCTTCTTCTTCGTCTTCTTCATCTTCTTCATTATTATATTCTGCGTTGTTAGGGTTTATGGATTTTCGCCACGCCCCCTCGGATCTTTCCTTCCGCTTAAGTTCTCATACCACATCTTCCATATCTAGAAGTTCAACTGGGGACAATTCCAGCTATTTGTGTTCCACGGGTTCCAATTCCTCGTACCCGTCGGATTTCCCTCAGCATAAGGATGAGAAATTCGATTTGGTGAAGGGGGTTGCGGATGATGATAGCGACGACGAGAAGTTTAGTCTTCTAGGGCAACCCTTGCGTGTGAAGAGACAGAGGGACGCTCATTCATTTCTTAATCAGGATTCTGCGAAACGGGCTGCAGTCACAGATGAATCGAGCCTTGACATGAGACGAGTTTTGGTAAAGGCATGGGGGAATCAGTCGCTTCGAATTGCTGACCCGGAAATTCATAATATTATGGTGAAGGAGAAGGAGAGACAATATAAGGGTATTGAATTAGTTGCTTCGGAAAATTTTGTTTGTAGGGCTGTGATGGAAGCCTTAGGCAGCCATTTAACGAATAAGTATTCTGAGGGAATGCCTGGCGCTAGATACTATACGGGTAATCAGTATATAGATGAGATTGAATTGCTCTGTTGTTCGAGAGCCTTGGCTGCTTTTGACCTCGATTCTGAGAAATGGGGTGTGAATGTCCAACCTTACTCTTGTACATCAGCAAATTTTGCTGTGTATACAGGTCTTTTGTCTCCGAAGGATCGAATTATGGGTTTAGATTCAGCATCCGGAGGGCATCTTAGTCATGGGTATTATTCTCCTGTTGGGAAAAAAATTTCAGCGACTTCTATTTTCTTTGAGAGTCTTCCTTATAGAGTGAACCCCCTAACTGGATATGTTGATTATGATAAGCTTGAAGAAAAGGCGCTCGATTATCGTCCGAAAATACTCATCTGTGGAGGAAGTTCGTATCCACGCGAGTGGGATTATGCTAGGTGTAGACAGATCGCGGACAAATGCGGGGCGGTTTTGATGTGTGATATGGCCCATATAAGCGGTTTGGTGGCAGCCAAGGAATGTGCTAGTCCGTTTGAATATTGTGATATAGTTACTTCAACAACACACAAAAGTCTTCGAGGGCCCAGAGGAGGAATCATATTTTTCAGGAGGGGTCTAAAATCAAGGAAGCAAGGCGTGCTTCTAGCTCGTGGAGATGACACTGGTACTGCTACTTATGATTTTGAGGACAGGATAAACTTTTCTGTTTTCCCGTCGTTACAAGGGGGTCCCCATAATAATCATATTGCTGCCCTGGCCATAGCGTTAAAGCAAGTAGCTTCTCCCGAGTATAGAGTATATATTCGACAGGTGAAGAAAAATGCGCAGGCTTTAGCATCAGCTTTACTAAGAAGAAGTTGCAGATTGGTAACTAATGGTACTGACAATCATTTGGTGCTGTGGGATCTTACTCCTTTGGGACTAACAGCCAAGAATTATGAGATGATCTGTGAGGCATGTCATATTACAGTCAACAAGAGTGCCATTTATGGTGACAATGGAGCCATCTCCCCTAGAGGAGTAAGAATAGGCACTCCTGCAATGACGACTAGAGGCTGTCTAGAGGCTGATTTTGAGACGATAGCTGAGATTCTTCTTAAAGCAGCTCATATCACAAAAATAGTCGTTCGTAGAGGAAAGCTTGGAAAATTACATAAAGGTATCATGAAGAATCTACAAAACAACAAAGATATTGTGGAGCTTCGGAACCAGGTCGAGGCCTTTGCTGCTTCATTTGCAATGCCAGGATTTGATATATGAATATATGAGCTTCTTGCTTGCTGCTATATACAACCACTTCATTTGGTAACACTAAATATGAATTCGTGTGCTTGTAAATTATAGGAAACTACTTAGTACGTAATACTGCACAAAAAGGTCCAAGTTTGACATGTATCATTCCTTTTATTGTAACAAGTGAAATCTCAGTTTATGACAGAGAAATTTTCAAGTCAAATATCTCCCATTTAGACGGTGGTTTTGTGTTGTGGGGTGTGTGTCTGTGGATAATTCAAAACCTCACATGAACTGATTATTCTGTCGTTTGTTTGCTGCAATATATACAATAGACTGTTTTGTCGCTG

Coding sequence (CDS)

ATGGATTTTCGCCACGCCCCCTCGGATCTTTCCTTCCGCTTAAGTTCTCATACCACATCTTCCATATCTAGAAGTTCAACTGGGGACAATTCCAGCTATTTGTGTTCCACGGGTTCCAATTCCTCGTACCCGTCGGATTTCCCTCAGCATAAGGATGAGAAATTCGATTTGGTGAAGGGGGTTGCGGATGATGATAGCGACGACGAGAAGTTTAGTCTTCTAGGGCAACCCTTGCGTGTGAAGAGACAGAGGGACGCTCATTCATTTCTTAATCAGGATTCTGCGAAACGGGCTGCAGTCACAGATGAATCGAGCCTTGACATGAGACGAGTTTTGGTAAAGGCATGGGGGAATCAGTCGCTTCGAATTGCTGACCCGGAAATTCATAATATTATGGTGAAGGAGAAGGAGAGACAATATAAGGGTATTGAATTAGTTGCTTCGGAAAATTTTGTTTGTAGGGCTGTGATGGAAGCCTTAGGCAGCCATTTAACGAATAAGTATTCTGAGGGAATGCCTGGCGCTAGATACTATACGGGTAATCAGTATATAGATGAGATTGAATTGCTCTGTTGTTCGAGAGCCTTGGCTGCTTTTGACCTCGATTCTGAGAAATGGGGTGTGAATGTCCAACCTTACTCTTGTACATCAGCAAATTTTGCTGTGTATACAGGTCTTTTGTCTCCGAAGGATCGAATTATGGGTTTAGATTCAGCATCCGGAGGGCATCTTAGTCATGGGTATTATTCTCCTGTTGGGAAAAAAATTTCAGCGACTTCTATTTTCTTTGAGAGTCTTCCTTATAGAGTGAACCCCCTAACTGGATATGTTGATTATGATAAGCTTGAAGAAAAGGCGCTCGATTATCGTCCGAAAATACTCATCTGTGGAGGAAGTTCGTATCCACGCGAGTGGGATTATGCTAGGTGTAGACAGATCGCGGACAAATGCGGGGCGGTTTTGATGTGTGATATGGCCCATATAAGCGGTTTGGTGGCAGCCAAGGAATGTGCTAGTCCGTTTGAATATTGTGATATAGTTACTTCAACAACACACAAAAGTCTTCGAGGGCCCAGAGGAGGAATCATATTTTTCAGGAGGGGTCTAAAATCAAGGAAGCAAGGCGTGCTTCTAGCTCGTGGAGATGACACTGGTACTGCTACTTATGATTTTGAGGACAGGATAAACTTTTCTGTTTTCCCGTCGTTACAAGGGGGTCCCCATAATAATCATATTGCTGCCCTGGCCATAGCGTTAAAGCAAGTAGCTTCTCCCGAGTATAGAGTATATATTCGACAGGTGAAGAAAAATGCGCAGGCTTTAGCATCAGCTTTACTAAGAAGAAGTTGCAGATTGGTAACTAATGGTACTGACAATCATTTGGTGCTGTGGGATCTTACTCCTTTGGGACTAACAGCCAAGAATTATGAGATGATCTGTGAGGCATGTCATATTACAGTCAACAAGAGTGCCATTTATGGTGACAATGGAGCCATCTCCCCTAGAGGAGTAAGAATAGGCACTCCTGCAATGACGACTAGAGGCTGTCTAGAGGCTGATTTTGAGACGATAGCTGAGATTCTTCTTAAAGCAGCTCATATCACAAAAATAGTCGTTCGTAGAGGAAAGCTTGGAAAATTACATAAAGGTATCATGAAGAATCTACAAAACAACAAAGATATTGTGGAGCTTCGGAACCAGGTCGAGGCCTTTGCTGCTTCATTTGCAATGCCAGGATTTGATATATGA

Protein sequence

MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKGVADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQSLRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSSYPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMICEACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVRRGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI*

Homology

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BLAST of CSPI04G18270.1 vs. ExPASy Swiss-Prot
Match: Q84WV0 (Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 8.6e-222
Identity = 386/577 (66.90%), Postives = 452/577 (78.34%), Query Frame = 0


Query: 15  SSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFP-------QHKDEKFDLVK------GV 74
           +S T +   R+   D+S  L    S  S P+ F        + K EK   V       G 
Sbjct: 19  ASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLEQKAEKTTTVDEPKKDGGG 78

Query: 75  ADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQSL 134
             D  +DE F +LG  + +KRQRD    L Q    + +   +S L+ RR  V+AWG+Q +
Sbjct: 79  GGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSIGDSDLESRRAAVRAWGDQPI 138

Query: 135 RIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGN 194
            +ADP+IH +M KEK+RQ +GIEL+ASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGN
Sbjct: 139 HLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGN 198

Query: 195 QYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSASG 254
           QYID+IE LC  RAL AF L+S+KWGVNVQPYSCTSANFAVYTGLL P +RIMGLDS SG
Sbjct: 199 QYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGERIMGLDSPSG 258

Query: 255 GHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSSY 314
           GH+SHGY +P GKKISA SIFFES PY+VNP TGY+DYDKLE+KALDYRPKILICGGSSY
Sbjct: 259 GHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRPKILICGGSSY 318

Query: 315 PREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGG 374
           PR+WD+AR RQIADKCGAVLMCDMAHISGLVA KEC++PF++CDIVTSTTHK LRGPRGG
Sbjct: 319 PRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTTHKGLRGPRGG 378

Query: 375 IIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQ 434
           IIF+RRG K RKQG   +  D   +  YD E++INF+VFPSLQGGPHNNHIAALAIALKQ
Sbjct: 379 IIFYRRGPKIRKQGHHSSHCDT--STHYDLEEKINFAVFPSLQGGPHNNHIAALAIALKQ 438

Query: 435 VASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMICE 494
           VA+PEY+ YI+Q+KKNAQALA+ALLRR CRLVT GTDNHL+LWDLTP+GLT K YE +CE
Sbjct: 439 VATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGKVYEKVCE 498

Query: 495 ACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVRR 554
            CHIT+NK+AI+GDNG ISP GVRIGTPAMTTRGC+E+DFET+A+ L+KAA IT  + R 
Sbjct: 499 MCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQITSALQR- 558

Query: 555 GKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMP 579
            + GK HK  +K+L  NKDI ELRN+VEAFA  + MP
Sbjct: 559 -EHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

[Close]

BLAST of CSPI04G18270.1 vs. ExPASy Swiss-Prot
Match: Q9LM59 (Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 1.2e-218
Identity = 368/532 (69.17%), Postives = 436/532 (81.95%), Query Frame = 0


Query: 53  EKFDLVKGVADDDS--DDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRR 112
           E+ D V G  DDD   ++E+F LLG P+++KR R  +S+    S+       +S ++ RR
Sbjct: 72  EEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVVDSGIESRR 131

Query: 113 VLVKAWGNQSLRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSE 172
            +V+AWGNQS+  ADPEIH  M KEK+RQ++GIEL+ASENFVCRAVMEALGSHLTNKYSE
Sbjct: 132 AVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSE 191

Query: 173 GMPGARYYTGNQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPK 232
           GMPGARYYTGNQYID+IE+LC  RALAAF L+ EKWGVNVQPYSCTSANFAV+TGLL P 
Sbjct: 192 GMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLMPG 251

Query: 233 DRIMGLDSASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYR 292
           +RIMGLDS SGGH+SHGYY+P GKK+S  SIFFES PY+V+P TGY+DYDKLEEKALDYR
Sbjct: 252 ERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALDYR 311

Query: 293 PKILICGGSSYPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTST 352
           PKILICGGSSYPR+W++ R R IADKCGAVLM DMA ISGLVAAKE  +PF+YCDIVTST
Sbjct: 312 PKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVTST 371

Query: 353 THKSLRGPRGGIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNN 412
           THKSLRGPRGGIIF++RGLK +KQ + L   +      YDFE++INFSVFPSLQGGPHNN
Sbjct: 372 THKSLRGPRGGIIFYKRGLKPKKQSINLNHCE--SNIQYDFEEKINFSVFPSLQGGPHNN 431

Query: 413 HIAALAIALKQVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLG 472
           HIAALAIALKQ ASPEY++Y+RQVKKNA+ALASAL+ R C+L+T GTDNHL+LWDLTPLG
Sbjct: 432 HIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLG 491

Query: 473 LTAKNYEMICEACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLK 532
           LT K YE +CE CHITVNK AI+ +NG ISP GVRIG+PAMT+RGCLE +FET+A+ L +
Sbjct: 492 LTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYR 551

Query: 533 AAHITKIVVRRGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 583
           AA I     R  + GKL K  +K++ + K+I +LRNQVEAFA  FAMP FD+
Sbjct: 552 AAQIASAAQR--EHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

[Close]

BLAST of CSPI04G18270.1 vs. ExPASy Swiss-Prot
Match: O23254 (Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 5.5e-176
Identity = 299/468 (63.89%), Postives = 364/468 (77.78%), Query Frame = 0


Query: 113 VKAWGNQSLRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGM 172
           V +WGN SL   DPEIH+++ KEK RQ +GIEL+ASENF   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 173 PGARYYTGNQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDR 232
           PG RYY GN++IDEIE LC SRAL AF  D   WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 233 IMGLDSASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPK 292
           IMGLD  SGGHL+HGYY+  GKKISATSI+FESLPY+VN  TGY+DYDKLEEKALD+RPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 293 ILICGGSSYPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTH 352
           +LICGGS+YPR+WDYAR R IADK GA+L+CDMAHISGLVAA+E A+PFEYCD+VT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 353 KSLRGPRGGIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHI 412
           KSLRGPR G+IF+R+G K  K+G           A YDFED+INF+VFP+LQGGPHN+ I
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKG-------QPEGAVYDFEDKINFAVFPALQGGPHNHQI 303

Query: 413 AALAIALKQVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLT 472
            ALA+ALKQ  +P ++VY +QVK NA AL + L+ +  ++VTNGT+NHLVLWDL PLGLT
Sbjct: 304 GALAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLT 363

Query: 473 AKNYEMICEACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAA 532
               E +C+ C IT+NK+A++GD+ A++P GVRIG PAMT+RG +E DFE I E L +A 
Sbjct: 364 GNKVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAV 423

Query: 533 HITKIVVRRGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGF 581
            +T  + +    GKL K   K L NNKD+ +L+  VE F+AS+ MPGF
Sbjct: 424 TLTLDIQK--TYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGF 462

[Close]

BLAST of CSPI04G18270.1 vs. ExPASy Swiss-Prot
Match: Q9SVM4 (Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 4.9e-169
Identity = 287/470 (61.06%), Postives = 354/470 (75.32%), Query Frame = 0


Query: 113 VKAWGNQSLRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGM 172
           V +WGN  L   DPEI++++ KEK RQ +GIEL+A+ENF   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 173 PGARYYTGNQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDR 232
           PG RYY G ++IDEIE LC SR+L AF  + EKWGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 233 IMGLDSASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPK 292
           IMGLD  SGGH++HGYYS  GK ISATSI+FE+LPY+V+  TGY+DYDKLEEKA+D+RPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 293 ILICGGSSYPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTH 352
           ++ICGG+SYPREWDYAR R +ADK GA L+CDMAH S LVAA+E A PFEYCD+VT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 353 KSLRGPRGGIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHI 412
           KSLRGPR G+IF+R+G K  K+G             YDF+ +IN +VFP+LQ GPHNN I
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKG-------QPEGEVYDFDAKINSAVFPALQSGPHNNKI 303

Query: 413 AALAIALKQVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLT 472
            ALA+ALKQV +P ++VY +QVK NA  LAS L+ +   LVT+GTDNHL+LWDL PLGLT
Sbjct: 304 GALAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLT 363

Query: 473 AKNYEMICEACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAA 532
               E +CE C+IT+N++A++GD   ++P GVRIGTPAMT+RG +E DFE I E L +A 
Sbjct: 364 GNKVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAV 423

Query: 533 HITKIVVRRGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 583
            IT  +  + + GK+ K   K L NNK+I E++  VE F   F MPGF I
Sbjct: 424 TITLDI--QEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFI 464

[Close]

BLAST of CSPI04G18270.1 vs. ExPASy Swiss-Prot
Match: Q54EW1 (Serine hydroxymethyltransferase 2 OS=Dictyostelium discoideum OX=44689 GN=shmt2 PE=3 SV=1)

HSP 1 Score: 539.7 bits (1389), Expect = 4.2e-152
Identity = 256/462 (55.41%), Postives = 344/462 (74.46%), Query Frame = 0


Query: 118 NQSLRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARY 177
           N+S+  +DPEI+++M+KEK+RQ+ G+EL+ASENF  RAVME++GS  TNKY+EG+PGARY
Sbjct: 31  NRSVSESDPEIYDLMMKEKQRQFTGLELIASENFTSRAVMESIGSCFTNKYAEGLPGARY 90

Query: 178 YTGNQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLD 237
           Y GN+ +D++E LC  RAL  F+L+ E+WGVNVQPYS ++ANFA +TGLL P DRIMGLD
Sbjct: 91  YGGNEVVDQLENLCIKRALETFNLNPEEWGVNVQPYSGSTANFAAFTGLLKPHDRIMGLD 150

Query: 238 SASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICG 297
             SGGHL+HGY +   KKISATSIFFES+PY+VN  TGYVDY+K+E  A  +RPK+LI G
Sbjct: 151 LPSGGHLTHGYQTD-KKKISATSIFFESMPYQVNE-TGYVDYNKMEANAALFRPKLLIAG 210

Query: 298 GSSYPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRG 357
            S+YPREWDY R R+IADK GA L+CDMAHISG+VA K+  SPF +CD+VT+TTHK+LRG
Sbjct: 211 ASAYPREWDYERMRKIADKHGAYLLCDMAHISGMVAGKQAISPFLFCDVVTTTTHKTLRG 270

Query: 358 PRGGIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAI 417
           PR G+IFFR+  +   +G ++   DD      D E+RINF+VFPS QGGPH N IA +A+
Sbjct: 271 PRAGLIFFRKTKRRDAKGNII---DD------DLENRINFAVFPSCQGGPHENTIAGIAV 330

Query: 418 ALKQVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYE 477
           ALK+ +SP+++ Y +QV++N+Q +   L +R   LVT GTDNHLVLWDL P G+T    E
Sbjct: 331 ALKEASSPDFQEYTKQVRRNSQTMGEELKKRGYSLVTEGTDNHLVLWDLRPQGITGSKIE 390

Query: 478 MICEACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKI 537
             C+  HITVNK+A+YGD  AI+P GVR+G PA+T+RG  E DF  + + L +   I+  
Sbjct: 391 KACDEAHITVNKNAVYGDTNAIAPGGVRLGAPALTSRGLKEQDFVKVVDFLDRVVKISLD 450

Query: 538 VVRRGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPG 580
           +  + K+GK      + + +N+D+ ++R +V+ F+  F MPG
Sbjct: 451 I--QSKVGKKMPDFQRAIADNQDLKQIRQEVKEFSTKFGMPG 479

[Close]

BLAST of CSPI04G18270.1 vs. ExPASy TrEMBL
Match: A0A0A0L3A9 (Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_4G377740 PE=3 SV=1)

HSP 1 Score: 1173.3 bits (3034), Expect = 0.0e+00
Identity = 581/582 (99.83%), Postives = 581/582 (99.83%), Query Frame = 0


Query: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG 60
           MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG
Sbjct: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG 60

Query: 61  VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS 120
           VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS
Sbjct: 61  VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS 120

Query: 121 LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240
           NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS
Sbjct: 181 NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGVLL RGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLTRGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420

Query: 421 QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480
           QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC
Sbjct: 421 QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480

Query: 481 EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR 540
           EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR
Sbjct: 481 EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR 540

Query: 541 RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 583
           RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI
Sbjct: 541 RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 582

[Close]

BLAST of CSPI04G18270.1 vs. ExPASy TrEMBL
Match: A0A5A7VB49 (Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold119G001500 PE=3 SV=1)

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 556/582 (95.53%), Postives = 566/582 (97.25%), Query Frame = 0


Query: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG 60
           MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDF Q KDEKFDLVKG
Sbjct: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFRQQKDEKFDLVKG 60

Query: 61  VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS 120
           V DDDSDDEKFSLLGQPLRVKRQRDAHSFL+QDSAKRAAV DE+SL+ RR  VK WGNQS
Sbjct: 61  VVDDDSDDEKFSLLGQPLRVKRQRDAHSFLDQDSAKRAAVKDEASLETRRASVKEWGNQS 120

Query: 121 LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIHNIM+KEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHNIMLKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240
           NQYIDEIELLCC+RALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS
Sbjct: 181 NQYIDEIELLCCARALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGVLL  GDD   ATYDFEDRINFSVFPSLQGGPHNNH+AALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLTHGDDNCNATYDFEDRINFSVFPSLQGGPHNNHVAALAIALK 420

Query: 421 QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480
           QVASPEYRVYIRQVKKNAQALASALLRR+CRLVTNGTDNHL+LWDLTPLGLTAKNYEMIC
Sbjct: 421 QVASPEYRVYIRQVKKNAQALASALLRRNCRLVTNGTDNHLLLWDLTPLGLTAKNYEMIC 480

Query: 481 EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR 540
           EACHITVNKSAIYGDNGAISP GVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIV+R
Sbjct: 481 EACHITVNKSAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVIR 540

Query: 541 RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 583
           RGKLGKLHK IMKNLQNNKDIVELRN+VE FAASFAMPGFDI
Sbjct: 541 RGKLGKLHKDIMKNLQNNKDIVELRNRVETFAASFAMPGFDI 582

[Close]

BLAST of CSPI04G18270.1 vs. ExPASy TrEMBL
Match: A0A1S3B9K5 (Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103487552 PE=3 SV=1)

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 556/582 (95.53%), Postives = 566/582 (97.25%), Query Frame = 0


Query: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG 60
           MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDF Q KDEKFDLVKG
Sbjct: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFRQQKDEKFDLVKG 60

Query: 61  VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS 120
           V DDDSDDEKFSLLGQPLRVKRQRDAHSFL+QDSAKRAAV DE+SL+ RR  VK WGNQS
Sbjct: 61  VVDDDSDDEKFSLLGQPLRVKRQRDAHSFLDQDSAKRAAVKDEASLETRRASVKEWGNQS 120

Query: 121 LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIHNIM+KEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHNIMLKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240
           NQYIDEIELLCC+RALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS
Sbjct: 181 NQYIDEIELLCCARALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGVLL  GDD   ATYDFEDRINFSVFPSLQGGPHNNH+AALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLTHGDDNCNATYDFEDRINFSVFPSLQGGPHNNHVAALAIALK 420

Query: 421 QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480
           QVASPEYRVYIRQVKKNAQALASALLRR+CRLVTNGTDNHL+LWDLTPLGLTAKNYEMIC
Sbjct: 421 QVASPEYRVYIRQVKKNAQALASALLRRNCRLVTNGTDNHLLLWDLTPLGLTAKNYEMIC 480

Query: 481 EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR 540
           EACHITVNKSAIYGDNGAISP GVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIV+R
Sbjct: 481 EACHITVNKSAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVIR 540

Query: 541 RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 583
           RGKLGKLHK IMKNLQNNKDIVELRN+VE FAASFAMPGFDI
Sbjct: 541 RGKLGKLHKDIMKNLQNNKDIVELRNRVETFAASFAMPGFDI 582

[Close]

BLAST of CSPI04G18270.1 vs. ExPASy TrEMBL
Match: A0A6J1IY33 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480261 PE=3 SV=1)

HSP 1 Score: 1025.8 bits (2651), Expect = 7.1e-296
Identity = 511/582 (87.80%), Postives = 535/582 (91.92%), Query Frame = 0


Query: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG 60
           MDFRHA SDLS R SSH TSSISRSS GDNSSYLCST SNSSY  +  + KDEKF+ VKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS 120
             DD SDDEKFSLLGQPLRVKRQRD+ SFLNQDSAKR A+ DESSL+ RR  V+AWGNQS
Sbjct: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120

Query: 121 LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIH+IM KEK+RQY+GIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240
           NQ+IDEIELLCC RALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYY+P GKKISA SIFF+SLPY+VNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDY RCRQIADKCGA+LM DM+HISGLVAAKEC SPF+YCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGVLL  GD+TG  TYDFEDRINFSVFPSLQGGPHNNHIAALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGDETG--TYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420

Query: 421 QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480
           QVAS EYRVYIRQVKKNAQALASALLRR CRLVT+GTDNHLVLWDL PLGLTAKNYE IC
Sbjct: 421 QVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKIC 480

Query: 481 EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR 540
           E CHITVNK AIYGDNGAISP GVRIGTPAMTTRGCLEADFETIAEILLKA  ITK+V+ 
Sbjct: 481 ELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLH 540

Query: 541 RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 583
           R + GKLHK IMKNLQNNKDI+ELRN+VEAFAASFAMPGFDI
Sbjct: 541 R-EHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

[Close]

BLAST of CSPI04G18270.1 vs. ExPASy TrEMBL
Match: A0A6J1FSB1 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446415 PE=3 SV=1)

HSP 1 Score: 1019.2 bits (2634), Expect = 6.7e-294
Identity = 506/582 (86.94%), Postives = 535/582 (91.92%), Query Frame = 0


Query: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG 60
           MDFRHA SDLS R SSH TSSISRSS GDNSSYLCST SNSSY  +  + KDEKF+ VKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS 120
             DD SDDE+FSLLGQPLRVKRQRD+ SFLNQDSAKR A+ DESSL++RR  V+AWGNQS
Sbjct: 61  DPDDHSDDEQFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEVRRASVRAWGNQS 120

Query: 121 LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIH+IM KEK+RQY+GIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240
           NQ+IDEIELLCC RALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYY+P GKKISA SIFF+SLPY+VNPLTGYVDYDKLEEKALDYRPKI+ICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKIVICGGSS 300

Query: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDY RCRQIADKCGA+LM DM+HISGLVAAKEC SPF+YCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGVLL  G++TG  TYDFEDRINFSVFPSLQGGPHNNHIAALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGEETG--TYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420

Query: 421 QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480
           QV S EYRVYIRQVKKNAQALASALLRR CRLVT+GTDNHLVLWDL PLGLTAKNYE IC
Sbjct: 421 QVTSLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKIC 480

Query: 481 EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR 540
           E CHITVNK AIYGDNGAISP GVRIGTPAMTTRGCLEADFETIAEILLKA  ITK+V+ 
Sbjct: 481 ELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLH 540

Query: 541 RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 583
           R + GKLHK +MKNLQNNKDI+ELRN+VEAFAASFAMPGFDI
Sbjct: 541 R-EHGKLHKDMMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

[Close]

BLAST of CSPI04G18270.1 vs. NCBI nr
Match: XP_004142612.1 (serine hydroxymethyltransferase 7 [Cucumis sativus] >KGN54611.1 hypothetical protein Csa_012900 [Cucumis sativus])

HSP 1 Score: 1173.3 bits (3034), Expect = 0.0e+00
Identity = 581/582 (99.83%), Postives = 581/582 (99.83%), Query Frame = 0


Query: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG 60
           MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG
Sbjct: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG 60

Query: 61  VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS 120
           VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS
Sbjct: 61  VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS 120

Query: 121 LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240
           NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS
Sbjct: 181 NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGVLL RGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLTRGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420

Query: 421 QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480
           QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC
Sbjct: 421 QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480

Query: 481 EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR 540
           EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR
Sbjct: 481 EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR 540

Query: 541 RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 583
           RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI
Sbjct: 541 RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 582

[Close]

BLAST of CSPI04G18270.1 vs. NCBI nr
Match: XP_008444112.1 (PREDICTED: serine hydroxymethyltransferase 7-like [Cucumis melo] >KAA0064247.1 serine hydroxymethyltransferase 7-like [Cucumis melo var. makuwa] >TYK18617.1 serine hydroxymethyltransferase 7-like [Cucumis melo var. makuwa])

HSP 1 Score: 1127.1 bits (2914), Expect = 0.0e+00
Identity = 556/582 (95.53%), Postives = 566/582 (97.25%), Query Frame = 0


Query: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG 60
           MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDF Q KDEKFDLVKG
Sbjct: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFRQQKDEKFDLVKG 60

Query: 61  VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS 120
           V DDDSDDEKFSLLGQPLRVKRQRDAHSFL+QDSAKRAAV DE+SL+ RR  VK WGNQS
Sbjct: 61  VVDDDSDDEKFSLLGQPLRVKRQRDAHSFLDQDSAKRAAVKDEASLETRRASVKEWGNQS 120

Query: 121 LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIHNIM+KEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHNIMLKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240
           NQYIDEIELLCC+RALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS
Sbjct: 181 NQYIDEIELLCCARALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGVLL  GDD   ATYDFEDRINFSVFPSLQGGPHNNH+AALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLTHGDDNCNATYDFEDRINFSVFPSLQGGPHNNHVAALAIALK 420

Query: 421 QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480
           QVASPEYRVYIRQVKKNAQALASALLRR+CRLVTNGTDNHL+LWDLTPLGLTAKNYEMIC
Sbjct: 421 QVASPEYRVYIRQVKKNAQALASALLRRNCRLVTNGTDNHLLLWDLTPLGLTAKNYEMIC 480

Query: 481 EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR 540
           EACHITVNKSAIYGDNGAISP GVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIV+R
Sbjct: 481 EACHITVNKSAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVIR 540

Query: 541 RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 583
           RGKLGKLHK IMKNLQNNKDIVELRN+VE FAASFAMPGFDI
Sbjct: 541 RGKLGKLHKDIMKNLQNNKDIVELRNRVETFAASFAMPGFDI 582

[Close]

BLAST of CSPI04G18270.1 vs. NCBI nr
Match: XP_038897933.1 (serine hydroxymethyltransferase 7-like [Benincasa hispida] >XP_038897934.1 serine hydroxymethyltransferase 7-like [Benincasa hispida])

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 546/582 (93.81%), Postives = 561/582 (96.39%), Query Frame = 0


Query: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG 60
           MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPS+F   KDEKFDLVKG
Sbjct: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSEFRHQKDEKFDLVKG 60

Query: 61  VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS 120
           V DDDS+DEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAV DE+SL+ RR LV+AWGNQS
Sbjct: 61  VVDDDSEDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVADEASLETRRALVRAWGNQS 120

Query: 121 LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           +RIADPEIHNIM KE++RQ KGIELVASENFVCRAVMEALGSHLTNKYSEGMPG+RYYTG
Sbjct: 121 IRIADPEIHNIMAKERQRQCKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGSRYYTG 180

Query: 181 NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240
           NQYIDEIELLCC+RALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDR+MGLDS S
Sbjct: 181 NQYIDEIELLCCARALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRLMGLDSTS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGVLL  GDDTGT TYDFEDRINFSVFPSLQGGPHNNHIAALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLTHGDDTGTGTYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420

Query: 421 QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480
           QVASPEY+VYIRQVKKNAQALASALLRR CRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC
Sbjct: 421 QVASPEYKVYIRQVKKNAQALASALLRRKCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480

Query: 481 EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR 540
           EACHITVNK AIYGDNGAISP GVRIGTPAMTTRGCLEADFETIAEILL+AA ITKIV+R
Sbjct: 481 EACHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLQAARITKIVIR 540

Query: 541 RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 583
           RGKLGKLHK IMKNLQNN DIVELRN+VEAFAASFAMPGFDI
Sbjct: 541 RGKLGKLHKDIMKNLQNNNDIVELRNRVEAFAASFAMPGFDI 582

[Close]

BLAST of CSPI04G18270.1 vs. NCBI nr
Match: XP_022980970.1 (serine hydroxymethyltransferase 7-like [Cucurbita maxima])

HSP 1 Score: 1025.8 bits (2651), Expect = 1.5e-295
Identity = 511/582 (87.80%), Postives = 535/582 (91.92%), Query Frame = 0


Query: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG 60
           MDFRHA SDLS R SSH TSSISRSS GDNSSYLCST SNSSY  +  + KDEKF+ VKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS 120
             DD SDDEKFSLLGQPLRVKRQRD+ SFLNQDSAKR A+ DESSL+ RR  V+AWGNQS
Sbjct: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120

Query: 121 LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIH+IM KEK+RQY+GIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240
           NQ+IDEIELLCC RALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLHPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYY+P GKKISA SIFF+SLPY+VNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDY RCRQIADKCGA+LM DM+HISGLVAAKEC SPF+YCDIVTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECVSPFQYCDIVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGVLL  GD+TG  TYDFEDRINFSVFPSLQGGPHNNHIAALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGDETG--TYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420

Query: 421 QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480
           QVAS EYRVYIRQVKKNAQALASALLRR CRLVT+GTDNHLVLWDL PLGLTAKNYE IC
Sbjct: 421 QVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKIC 480

Query: 481 EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR 540
           E CHITVNK AIYGDNGAISP GVRIGTPAMTTRGCLEADFETIAEILLKA  ITK+V+ 
Sbjct: 481 ELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLH 540

Query: 541 RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 583
           R + GKLHK IMKNLQNNKDI+ELRN+VEAFAASFAMPGFDI
Sbjct: 541 R-EHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

[Close]

BLAST of CSPI04G18270.1 vs. NCBI nr
Match: KAG7031702.1 (Serine hydroxymethyltransferase 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1025.0 bits (2649), Expect = 2.5e-295
Identity = 510/582 (87.63%), Postives = 536/582 (92.10%), Query Frame = 0


Query: 1   MDFRHAPSDLSFRLSSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFPQHKDEKFDLVKG 60
           MDFRHA SDLS R SSH TSSISRSS GDNSSYLCST SNSSY  +  + KDEKF+ VKG
Sbjct: 1   MDFRHAQSDLSLRFSSHATSSISRSSIGDNSSYLCSTASNSSYLLELREQKDEKFNFVKG 60

Query: 61  VADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQS 120
             DD SDDEKFSLLGQPLRVKRQRD+ SFLNQDSAKR A+ DESSL+ RR  V+AWGNQS
Sbjct: 61  DPDDHSDDEKFSLLGQPLRVKRQRDSQSFLNQDSAKRLAIMDESSLEARRASVRAWGNQS 120

Query: 121 LRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180
           LRIADPEIH+IM KEK+RQY+GIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG
Sbjct: 121 LRIADPEIHDIMAKEKQRQYRGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTG 180

Query: 181 NQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSAS 240
           NQ+IDEIELLCC RALAAFDLD EKWGVNVQPYSCTSANFAVYTGLL PKDRIMGLDSAS
Sbjct: 181 NQHIDEIELLCCERALAAFDLDPEKWGVNVQPYSCTSANFAVYTGLLLPKDRIMGLDSAS 240

Query: 241 GGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300
           GGHLSHGYY+P GKKISA SIFF+SLPY+VNPLTGYVDYDKLEEKALDYRPKILICGGSS
Sbjct: 241 GGHLSHGYYTPGGKKISAASIFFQSLPYKVNPLTGYVDYDKLEEKALDYRPKILICGGSS 300

Query: 301 YPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRG 360
           YPREWDY RCRQIADKCGA+LM DM+HISGLVAAKECASPF+YCD+VTSTTHKSLRGPRG
Sbjct: 301 YPREWDYGRCRQIADKCGAILMSDMSHISGLVAAKECASPFQYCDVVTSTTHKSLRGPRG 360

Query: 361 GIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420
           GIIFFRRGLKSRKQGVLL  G++TG  TYDFEDRINFSVFPSLQGGPHNNHIAALAIALK
Sbjct: 361 GIIFFRRGLKSRKQGVLLNHGEETG--TYDFEDRINFSVFPSLQGGPHNNHIAALAIALK 420

Query: 421 QVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMIC 480
           QVAS EYRVYIRQVKKNAQALASALLRR CRLVT+GTDNHLVLWDL PLGLTAKNYE IC
Sbjct: 421 QVASLEYRVYIRQVKKNAQALASALLRRKCRLVTDGTDNHLVLWDLIPLGLTAKNYEKIC 480

Query: 481 EACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVR 540
           E CHITVNK AIYGDNGAISP GVRIGTPAMTTRGCLEADFETIAEILLKA  ITK+V+ 
Sbjct: 481 ELCHITVNKCAIYGDNGAISPGGVRIGTPAMTTRGCLEADFETIAEILLKAVQITKMVLH 540

Query: 541 RGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 583
           R + GKLHK IMKNLQNNKDI+ELRN+VEAFAASFAMPGFDI
Sbjct: 541 R-EHGKLHKDIMKNLQNNKDIMELRNRVEAFAASFAMPGFDI 579

[Close]

BLAST of CSPI04G18270.1 vs. TAIR 10
Match: AT1G36370.1 (serine hydroxymethyltransferase 7 )

HSP 1 Score: 771.2 bits (1990), Expect = 6.1e-223
Identity = 386/577 (66.90%), Postives = 452/577 (78.34%), Query Frame = 0


Query: 15  SSHTTSSISRSSTGDNSSYLCSTGSNSSYPSDFP-------QHKDEKFDLVK------GV 74
           +S T +   R+   D+S  L    S  S P+ F        + K EK   V       G 
Sbjct: 19  ASMTPTPTPRAPIADDSINLQVDQSFRSLPTTFSPIPLQLLEQKAEKTTTVDEPKKDGGG 78

Query: 75  ADDDSDDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRRVLVKAWGNQSL 134
             D  +DE F +LG  + +KRQRD    L Q    + +   +S L+ RR  V+AWG+Q +
Sbjct: 79  GGDQKEDEHFRILGHHMCLKRQRDCPLLLTQSKHPKRSSIGDSDLESRRAAVRAWGDQPI 138

Query: 135 RIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGN 194
            +ADP+IH +M KEK+RQ +GIEL+ASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGN
Sbjct: 139 HLADPDIHELMEKEKQRQVRGIELIASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGN 198

Query: 195 QYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSASG 254
           QYID+IE LC  RAL AF L+S+KWGVNVQPYSCTSANFAVYTGLL P +RIMGLDS SG
Sbjct: 199 QYIDQIENLCIERALTAFGLESDKWGVNVQPYSCTSANFAVYTGLLLPGERIMGLDSPSG 258

Query: 255 GHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSSY 314
           GH+SHGY +P GKKISA SIFFES PY+VNP TGY+DYDKLE+KALDYRPKILICGGSSY
Sbjct: 259 GHMSHGYCTPGGKKISAASIFFESFPYKVNPQTGYIDYDKLEDKALDYRPKILICGGSSY 318

Query: 315 PREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGG 374
           PR+WD+AR RQIADKCGAVLMCDMAHISGLVA KEC++PF++CDIVTSTTHK LRGPRGG
Sbjct: 319 PRDWDFARVRQIADKCGAVLMCDMAHISGLVATKECSNPFDHCDIVTSTTHKGLRGPRGG 378

Query: 375 IIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQ 434
           IIF+RRG K RKQG   +  D   +  YD E++INF+VFPSLQGGPHNNHIAALAIALKQ
Sbjct: 379 IIFYRRGPKIRKQGHHSSHCDT--STHYDLEEKINFAVFPSLQGGPHNNHIAALAIALKQ 438

Query: 435 VASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMICE 494
           VA+PEY+ YI+Q+KKNAQALA+ALLRR CRLVT GTDNHL+LWDLTP+GLT K YE +CE
Sbjct: 439 VATPEYKAYIQQMKKNAQALAAALLRRKCRLVTGGTDNHLLLWDLTPMGLTGKVYEKVCE 498

Query: 495 ACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHITKIVVRR 554
            CHIT+NK+AI+GDNG ISP GVRIGTPAMTTRGC+E+DFET+A+ L+KAA IT  + R 
Sbjct: 499 MCHITLNKTAIFGDNGTISPGGVRIGTPAMTTRGCIESDFETMADFLIKAAQITSALQR- 558

Query: 555 GKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMP 579
            + GK HK  +K+L  NKDI ELRN+VEAFA  + MP
Sbjct: 559 -EHGKSHKEFVKSLCTNKDIAELRNRVEAFALQYEMP 591

[Close]

BLAST of CSPI04G18270.1 vs. TAIR 10
Match: AT1G22020.1 (serine hydroxymethyltransferase 6 )

HSP 1 Score: 760.8 bits (1963), Expect = 8.2e-220
Identity = 368/532 (69.17%), Postives = 436/532 (81.95%), Query Frame = 0


Query: 53  EKFDLVKGVADDDS--DDEKFSLLGQPLRVKRQRDAHSFLNQDSAKRAAVTDESSLDMRR 112
           E+ D V G  DDD   ++E+F LLG P+++KR R  +S+    S+       +S ++ RR
Sbjct: 72  EEDDEVVGDNDDDDQREEEQFILLGHPMKLKRGRGGNSYSLASSSPCKRFVVDSGIESRR 131

Query: 113 VLVKAWGNQSLRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSE 172
            +V+AWGNQS+  ADPEIH  M KEK+RQ++GIEL+ASENFVCRAVMEALGSHLTNKYSE
Sbjct: 132 AVVRAWGNQSIEEADPEIHEFMEKEKQRQFRGIELIASENFVCRAVMEALGSHLTNKYSE 191

Query: 173 GMPGARYYTGNQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPK 232
           GMPGARYYTGNQYID+IE+LC  RALAAF L+ EKWGVNVQPYSCTSANFAV+TGLL P 
Sbjct: 192 GMPGARYYTGNQYIDQIEILCQERALAAFGLNHEKWGVNVQPYSCTSANFAVFTGLLMPG 251

Query: 233 DRIMGLDSASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYR 292
           +RIMGLDS SGGH+SHGYY+P GKK+S  SIFFES PY+V+P TGY+DYDKLEEKALDYR
Sbjct: 252 ERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFPYKVDPRTGYIDYDKLEEKALDYR 311

Query: 293 PKILICGGSSYPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTST 352
           PKILICGGSSYPR+W++ R R IADKCGAVLM DMA ISGLVAAKE  +PF+YCDIVTST
Sbjct: 312 PKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQISGLVAAKESPNPFDYCDIVTST 371

Query: 353 THKSLRGPRGGIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNN 412
           THKSLRGPRGGIIF++RGLK +KQ + L   +      YDFE++INFSVFPSLQGGPHNN
Sbjct: 372 THKSLRGPRGGIIFYKRGLKPKKQSINLNHCE--SNIQYDFEEKINFSVFPSLQGGPHNN 431

Query: 413 HIAALAIALKQVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLG 472
           HIAALAIALKQ ASPEY++Y+RQVKKNA+ALASAL+ R C+L+T GTDNHL+LWDLTPLG
Sbjct: 432 HIAALAIALKQAASPEYKLYMRQVKKNAKALASALISRKCKLITGGTDNHLLLWDLTPLG 491

Query: 473 LTAKNYEMICEACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLK 532
           LT K YE +CE CHITVNK AI+ +NG ISP GVRIG+PAMT+RGCLE +FET+A+ L +
Sbjct: 492 LTGKVYEKVCEMCHITVNKVAIFSENGVISPGGVRIGSPAMTSRGCLEPEFETMADFLYR 551

Query: 533 AAHITKIVVRRGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 583
           AA I     R  + GKL K  +K++ + K+I +LRNQVEAFA  FAMP FD+
Sbjct: 552 AAQIASAAQR--EHGKLQKEPLKSIYHCKEIADLRNQVEAFATQFAMPAFDM 599

[Close]

BLAST of CSPI04G18270.1 vs. TAIR 10
Match: AT4G13930.1 (serine hydroxymethyltransferase 4 )

HSP 1 Score: 619.0 bits (1595), Expect = 3.9e-177
Identity = 299/468 (63.89%), Postives = 364/468 (77.78%), Query Frame = 0


Query: 113 VKAWGNQSLRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGM 172
           V +WGN SL   DPEIH+++ KEK RQ +GIEL+ASENF   AV+EALGS LTNKYSEG+
Sbjct: 4   VSSWGNTSLVSVDPEIHDLIEKEKRRQCRGIELIASENFTSFAVIEALGSALTNKYSEGI 63

Query: 173 PGARYYTGNQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDR 232
           PG RYY GN++IDEIE LC SRAL AF  D   WGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGNEFIDEIENLCRSRALEAFHCDPAAWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 233 IMGLDSASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPK 292
           IMGLD  SGGHL+HGYY+  GKKISATSI+FESLPY+VN  TGY+DYDKLEEKALD+RPK
Sbjct: 124 IMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLPYKVNFTTGYIDYDKLEEKALDFRPK 183

Query: 293 ILICGGSSYPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTH 352
           +LICGGS+YPR+WDYAR R IADK GA+L+CDMAHISGLVAA+E A+PFEYCD+VT+TTH
Sbjct: 184 LLICGGSAYPRDWDYARFRAIADKVGALLLCDMAHISGLVAAQEAANPFEYCDVVTTTTH 243

Query: 353 KSLRGPRGGIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHI 412
           KSLRGPR G+IF+R+G K  K+G           A YDFED+INF+VFP+LQGGPHN+ I
Sbjct: 244 KSLRGPRAGMIFYRKGPKPPKKG-------QPEGAVYDFEDKINFAVFPALQGGPHNHQI 303

Query: 413 AALAIALKQVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLT 472
            ALA+ALKQ  +P ++VY +QVK NA AL + L+ +  ++VTNGT+NHLVLWDL PLGLT
Sbjct: 304 GALAVALKQANTPGFKVYAKQVKANAVALGNYLMSKGYQIVTNGTENHLVLWDLRPLGLT 363

Query: 473 AKNYEMICEACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAA 532
               E +C+ C IT+NK+A++GD+ A++P GVRIG PAMT+RG +E DFE I E L +A 
Sbjct: 364 GNKVEKLCDLCSITLNKNAVFGDSSALAPGGVRIGAPAMTSRGLVEKDFEQIGEFLSRAV 423

Query: 533 HITKIVVRRGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGF 581
            +T  + +    GKL K   K L NNKD+ +L+  VE F+AS+ MPGF
Sbjct: 424 TLTLDIQK--TYGKLLKDFNKGLVNNKDLDQLKADVEKFSASYEMPGF 462

[Close]

BLAST of CSPI04G18270.1 vs. TAIR 10
Match: AT4G13890.1 (Pyridoxal phosphate (PLP)-dependent transferases superfamily protein )

HSP 1 Score: 595.9 bits (1535), Expect = 3.5e-170
Identity = 287/470 (61.06%), Postives = 354/470 (75.32%), Query Frame = 0


Query: 113 VKAWGNQSLRIADPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGM 172
           V +WGN  L   DPEI++++ KEK RQ +GIEL+A+ENF   AVMEALGS LTNKYSEGM
Sbjct: 4   VYSWGNTHLDFVDPEIYDLIEKEKHRQCRGIELIAAENFTSVAVMEALGSCLTNKYSEGM 63

Query: 173 PGARYYTGNQYIDEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDR 232
           PG RYY G ++IDEIE LC SR+L AF  + EKWGVNVQPYS + ANFA YT LL P DR
Sbjct: 64  PGNRYYGGTEFIDEIESLCRSRSLEAFHCNPEKWGVNVQPYSGSPANFAAYTALLQPHDR 123

Query: 233 IMGLDSASGGHLSHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPK 292
           IMGLD  SGGH++HGYYS  GK ISATSI+FE+LPY+V+  TGY+DYDKLEEKA+D+RPK
Sbjct: 124 IMGLDLPSGGHITHGYYSSGGKNISATSIYFENLPYKVDSKTGYIDYDKLEEKAMDFRPK 183

Query: 293 ILICGGSSYPREWDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTH 352
           ++ICGG+SYPREWDYAR R +ADK GA L+CDMAH S LVAA+E A PFEYCD+VT++TH
Sbjct: 184 LIICGGTSYPREWDYARFRAVADKVGAFLLCDMAHNSALVAAQEAADPFEYCDVVTTSTH 243

Query: 353 KSLRGPRGGIIFFRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHI 412
           KSLRGPR G+IF+R+G K  K+G             YDF+ +IN +VFP+LQ GPHNN I
Sbjct: 244 KSLRGPRAGMIFYRKGPKPAKKG-------QPEGEVYDFDAKINSAVFPALQSGPHNNKI 303

Query: 413 AALAIALKQVASPEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLT 472
            ALA+ALKQV +P ++VY +QVK NA  LAS L+ +   LVT+GTDNHL+LWDL PLGLT
Sbjct: 304 GALAVALKQVMAPSFKVYAKQVKANAACLASYLINKGYTLVTDGTDNHLILWDLRPLGLT 363

Query: 473 AKNYEMICEACHITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAA 532
               E +CE C+IT+N++A++GD   ++P GVRIGTPAMT+RG +E DFE I E L +A 
Sbjct: 364 GNKVEKVCELCYITLNRNAVFGDTSFLAPGGVRIGTPAMTSRGLVEKDFEKIGEFLHRAV 423

Query: 533 HITKIVVRRGKLGKLHKGIMKNLQNNKDIVELRNQVEAFAASFAMPGFDI 583
            IT  +  + + GK+ K   K L NNK+I E++  VE F   F MPGF I
Sbjct: 424 TITLDI--QEQYGKVMKDFNKGLVNNKEIDEIKADVEEFTYDFDMPGFFI 464

[Close]

BLAST of CSPI04G18270.1 vs. TAIR 10
Match: AT4G32520.1 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 480.7 bits (1236), Expect = 1.6e-135
Identity = 244/459 (53.16%), Postives = 314/459 (68.41%), Query Frame = 0


Query: 125 DPEIHNIMVKEKERQYKGIELVASENFVCRAVMEALGSHLTNKYSEGMPGARYYTGNQYI 184
           DPE+  I+ KEK+RQ++ +EL+ASENF  RAVMEA+GS LTNKYSEG+PG RYY GN+YI
Sbjct: 87  DPEVRTIITKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGGNEYI 146

Query: 185 DEIELLCCSRALAAFDLDSEKWGVNVQPYSCTSANFAVYTGLLSPKDRIMGLDSASGGHL 244
           D++E LC +RALAAF LDS KWGVNVQP S + ANFAVYT +LSP DRIMGLD   GGHL
Sbjct: 147 DQLETLCQNRALAAFRLDSTKWGVNVQPLSGSPANFAVYTAILSPHDRIMGLDLPHGGHL 206

Query: 245 SHGYYSPVGKKISATSIFFESLPYRVNPLTGYVDYDKLEEKALDYRPKILICGGSSYPRE 304
           SHG+ +   +++S TSI+FES+PYR++  TG VDYD LE+ A  +RPK++I G S+Y R+
Sbjct: 207 SHGFMT-AKRRVSGTSIYFESMPYRLDESTGIVDYDMLEKTATLFRPKLIIAGASAYSRD 266

Query: 305 WDYARCRQIADKCGAVLMCDMAHISGLVAAKECASPFEYCDIVTSTTHKSLRGPRGGIIF 364
           +DY R R+IAD  GA LM DMAHISGLVAA   A PFEYCDIVT+TTHKSLRGPRGG+IF
Sbjct: 267 FDYPRMRKIADSVGAFLMMDMAHISGLVAASVVADPFEYCDIVTTTTHKSLRGPRGGMIF 326

Query: 365 FRRGLKSRKQGVLLARGDDTGTATYDFEDRINFSVFPSLQGGPHNNHIAALAIALKQVAS 424
           FR   K    GV             D E  +N +VFP LQGGPHN+ I  LA+ LK   S
Sbjct: 327 FR---KDPINGV-------------DLESAVNNAVFPGLQGGPHNHTIGGLAVCLKHAQS 386

Query: 425 PEYRVYIRQVKKNAQALASALLRRSCRLVTNGTDNHLVLWDLTPLGLTAKNYEMICEACH 484
           PE++ Y ++V  N +ALA+ L+    +LV+ G+DNHLVL DL P+G+     E I +   
Sbjct: 387 PEFKAYQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGARVEKILDMAS 446

Query: 485 ITVNKSAIYGDNGAISPRGVRIGTPAMTTRGCLEADFETIAEILLKAAHIT---KIVVRR 544
           IT+NK+++ GD  A+ P G+RIG+PAMTTRG  E DF  +A+ + +   IT   K     
Sbjct: 447 ITLNKNSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEITMEAKKAAPG 506

Query: 545 GKLGKLHKGIMK-NLQNNKDIVELRNQVEAFAASFAMPG 580
            KL   +K +        + +  L+ +VE F + F +PG
Sbjct: 507 SKLQDFNKFVTSPEFPLKERVKSLKERVETFTSRFPIPG 528

The following BLAST results are available for this feature:

BLAST of CSPI04G18270.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84WV0	8.6e-222	66.90	Serine hydroxymethyltransferase 7 OS=Arabidopsis thaliana OX=3702 GN=SHM7 PE=2 S...	[more]
Q9LM59	1.2e-218	69.17	Serine hydroxymethyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=SHM6 PE=2 S...	[more]
O23254	5.5e-176	63.89	Serine hydroxymethyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=SHM4 PE=1 S...	[more]
Q9SVM4	4.9e-169	61.06	Serine hydroxymethyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=SHM5 PE=3 S...	[more]
Q54EW1	4.2e-152	55.41	Serine hydroxymethyltransferase 2 OS=Dictyostelium discoideum OX=44689 GN=shmt2 ...	[more]

BLAST of CSPI04G18270.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3A9	0.0e+00	99.83	Serine hydroxymethyltransferase OS=Cucumis sativus OX=3659 GN=Csa_4G377740 PE=3 ...	[more]
A0A5A7VB49	0.0e+00	95.53	Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3B9K5	0.0e+00	95.53	Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103487552 PE=3 SV=...	[more]
A0A6J1IY33	7.1e-296	87.80	Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111480261 PE=3...	[more]
A0A6J1FSB1	6.7e-294	86.94	Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111446415 PE...	[more]

BLAST of CSPI04G18270.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004142612.1	0.0e+00	99.83	serine hydroxymethyltransferase 7 [Cucumis sativus] >KGN54611.1 hypothetical pro...	[more]
XP_008444112.1	0.0e+00	95.53	PREDICTED: serine hydroxymethyltransferase 7-like [Cucumis melo] >KAA0064247.1 s...	[more]
XP_038897933.1	0.0e+00	93.81	serine hydroxymethyltransferase 7-like [Benincasa hispida] >XP_038897934.1 serin...	[more]
XP_022980970.1	1.5e-295	87.80	serine hydroxymethyltransferase 7-like [Cucurbita maxima]	[more]
KAG7031702.1	2.5e-295	87.63	Serine hydroxymethyltransferase 6 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CSPI04G18270.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G36370.1	6.1e-223	66.90	serine hydroxymethyltransferase 7	[more]
AT1G22020.1	8.2e-220	69.17	serine hydroxymethyltransferase 6	[more]
AT4G13930.1	3.9e-177	63.89	serine hydroxymethyltransferase 4	[more]
AT4G13890.1	3.5e-170	61.06	Pyridoxal phosphate (PLP)-dependent transferases superfamily protein	[more]
AT4G32520.1	1.6e-135	53.16	serine hydroxymethyltransferase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015421	Pyridoxal phosphate-dependent transferase, major domain	GENE3D	3.40.640.10		coord: 150..432 e-value: 4.1E-193 score: 643.7
IPR039429	Serine hydroxymethyltransferase-like domain	PFAM	PF00464	SHMT	coord: 123..527 e-value: 2.4E-170 score: 566.4
IPR015422	Pyridoxal phosphate-dependent transferase, small domain	GENE3D	3.90.1150.10	Aspartate Aminotransferase, domain 1	coord: 125..578 e-value: 4.1E-193 score: 643.7
None	No IPR available	PIRSR	PIRSR000412-50	PIRSR000412-50	coord: 120..580 e-value: 2.9E-167 score: 554.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..48
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 11..46
None	No IPR available	PANTHER	PTHR11680:SF7	SERINE HYDROXYMETHYLTRANSFERASE 7	coord: 83..582
IPR001085	Serine hydroxymethyltransferase	PANTHER	PTHR11680	SERINE HYDROXYMETHYLTRANSFERASE	coord: 83..582
IPR001085	Serine hydroxymethyltransferase	HAMAP	MF_00051	SHMT	coord: 120..579 score: 39.250679
IPR001085	Serine hydroxymethyltransferase	CDD	cd00378	SHMT	coord: 125..571 e-value: 0.0 score: 562.979
IPR019798	Serine hydroxymethyltransferase, pyridoxal phosphate binding site	PROSITE	PS00096	SHMT	coord: 345..361
IPR015424	Pyridoxal phosphate-dependent transferase	SUPERFAMILY	53383	PLP-dependent transferases	coord: 109..581

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI04G18270	CSPI04G18270	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G18270.1.utr5p1	CSPI04G18270.1.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G18270.1.cds1	CSPI04G18270.1.cds1	CDS
CSPI04G18270.1.cds2	CSPI04G18270.1.cds2	CDS
CSPI04G18270.1.cds3	CSPI04G18270.1.cds3	CDS
CSPI04G18270.1.cds4	CSPI04G18270.1.cds4	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G18270.1.utr3p1	CSPI04G18270.1.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI04G18270.1	CSPI04G18270.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019264	glycine biosynthetic process from serine
biological_process	GO:0035999	tetrahydrofolate interconversion
biological_process	GO:0046655	folic acid metabolic process
biological_process	GO:0006565	L-serine catabolic process
biological_process	GO:0032259	methylation
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004372	glycine hydroxymethyltransferase activity
molecular_function	GO:0030170	pyridoxal phosphate binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0070905	serine binding
molecular_function	GO:0008270	zinc ion binding