CSPI04G16180.2 (mRNA) Cucumber (PI 183967) v1

BLAST of CSPI04G16180.2 vs. ExPASy Swiss-Prot
Match: Q84K71 (Protein DETOXIFICATION 44, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX44 PE=2 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 3.1e-160
Identity = 309/509 (60.71%), Postives = 387/509 (76.03%), Query Frame = 0

Query: 39  PKPSFHRNLTTSSLKSPLEESKSTPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDE 98
           P  S  R +   S  SP +ES +  +S Q    RP+   +        +          +
Sbjct: 22  PNSSIRRTIVCKS--SPRDESPAVSTSSQ----RPEKQQNPLTSQNKPDHDHKPDPGIGK 81

Query: 99  LALDILAIALPAALALAADPIASLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLL 158
           + ++I++IALPAALALAADPI SL+DTAFVGHIGS ELAA+GVS SVFNLVSKLFNVPLL
Sbjct: 82  IGMEIMSIALPAALALAADPITSLVDTAFVGHIGSAELAAVGVSVSVFNLVSKLFNVPLL 141

Query: 159 NITTSFVAEEQALINADEKNIVQTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLS 218
           N+TTSFVAEEQA+   D+ + ++T          KK+L SVSTSL LA G+GIAEA+ LS
Sbjct: 142 NVTTSFVAEEQAIAAKDDNDSIET---------SKKVLPSVSTSLVLAAGVGIAEAIALS 201

Query: 219 LGSGTLMDIMGIPVDSSMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATA 278
           LGS  LMD+M IP DS MR PAEQFL LRA+GAPPIV+ALAAQG FRGFKDT TPLYA  
Sbjct: 202 LGSDFLMDVMAIPFDSPMRIPAEQFLRLRAYGAPPIVVALAAQGAFRGFKDTTTPLYAVV 261

Query: 279 AGNLLNAVLDPLLIFFCGFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIA 338
           AGN+LNAVLDP+LIF  GFGI GAA ATVISEYLIAF+LLW+LN  +      I  GR  
Sbjct: 262 AGNVLNAVLDPILIFVLGFGISGAAAATVISEYLIAFILLWKLNENVVLLSPQIKVGRAN 321

Query: 339 RYLQSGGLLMARTLAVLVTLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQ 398
           +YL+SGGLL+ RT+A+LV  TLATS+AA+ GP  MAG+QI ++IW+A+SLLTDALA+A Q
Sbjct: 322 QYLKSGGLLIGRTVALLVPFTLATSLAAQNGPTQMAGHQIVLEIWLAVSLLTDALAIAAQ 381

Query: 399 ALLAGSFTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARS 458
           +LLA +++  +Y+ +R+V++  LQ+GL +G  LA +LF+ F  FS LF+ D+EVL+ A S
Sbjct: 382 SLLATTYSQGEYKQAREVLFGVLQVGLATGTGLAAVLFITFEPFSSLFTTDSEVLKIALS 441

Query: 459 GLLFVAGSQPVNALAFVVDGLYYGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWS 518
           G LFVAGSQPVNALAFV+DGLYYGVSDFG+AAYSMV+VG +SS+++LV  PTFGL G+W+
Sbjct: 442 GTLFVAGSQPVNALAFVLDGLYYGVSDFGFAAYSMVIVGFISSLFMLVAAPTFGLAGIWT 501

Query: 519 GLFLFMMLRLVAGVWRLGTKSGPWELVFN 548
           GLFLFM LRLVAG WRLGT++GPW+++++
Sbjct: 502 GLFLFMALRLVAGAWRLGTRTGPWKMLWS 515

BLAST of CSPI04G16180.2 vs. ExPASy Swiss-Prot
Match: Q9SVE7 (Protein DETOXIFICATION 45, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX45 PE=2 SV=2)

HSP 1 Score: 399.4 bits (1025), Expect = 6.5e-110
Identity = 237/505 (46.93%), Postives = 329/505 (65.15%), Query Frame = 0

Query: 40  KPSFHRNLTTSSLKSPLEESKSTPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDEL 99
           K S H N           +S++ P    V     D++ S       VN       +  ++
Sbjct: 46  KCSLHTNPNPMFPFVTRRKSQTNPDCGVVKLGEEDDSCSSLDKLPEVNGVHTGVARPVDI 105

Query: 100 ALDILAIALPAALALAADPIASLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLN 159
             +++ ++LPA    A DP+  L++TA++G +GS EL + GVS ++FN +SKLFN+PLL+
Sbjct: 106 KRELVMLSLPAIAGQAIDPLTLLMETAYIGRLGSVELGSAGVSMAIFNTISKLFNIPLLS 165

Query: 160 ITTSFVAEEQALINADE--KNIVQTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVML 219
           + TSFVAE+ A I A +      Q+DI + +   E+K LSSVST+L LA G+GI EA+ L
Sbjct: 166 VATSFVAEDIAKIAAQDLASEDSQSDIPS-QGLPERKQLSSVSTALVLAIGIGIFEALAL 225

Query: 220 SLGSGTLMDIMGIPVDSSMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYAT 279
           SL SG  + +MGI   S M  PA QFL LRA GAP  V++LA QG FRGFKDTKTP+Y  
Sbjct: 226 SLASGPFLRLMGIQSMSEMFIPARQFLVLRALGAPAYVVSLALQGIFRGFKDTKTPVYCL 285

Query: 280 AAGNLLNAVLDPLLIFFCGFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRI 339
             GN L   L PL I+    G+ GAAI++VIS+Y +A ++L  LN  +      I   + 
Sbjct: 286 GIGNFLAVFLFPLFIYKFRMGVAGAAISSVISQYTVAILMLILLNKRVILLPPKIGSLKF 345

Query: 340 ARYLQSGGLLMARTLAVLVTLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAG 399
             YL+SGG ++ RTL+VLVT+T+ATSMAAR+G   MA +QIC+Q+W+A+SLLTDALA +G
Sbjct: 346 GDYLKSGGFVLGRTLSVLVTMTVATSMAARQGVFAMAAHQICMQVWLAVSLLTDALASSG 405

Query: 400 QALLAGSFTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETAR 459
           QAL+A S + +D+E  ++V    L+IG+++GI+LAI+L + F + +GLFS D EVL   R
Sbjct: 406 QALIASSASKRDFEGVKEVTTFVLKIGVVTGIALAIVLGMSFSSIAGLFSKDPEVLRIVR 465

Query: 460 SGLLFVAGSQPVNALAFVVDGLYYGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVW 519
            G+LFVA +QP+ ALAF+ DGL+YG+SDF YAA SM++VG +SS ++L      GL GVW
Sbjct: 466 KGVLFVAATQPITALAFIFDGLHYGMSDFPYAACSMMVVGGISSAFMLYAPAGLGLSGVW 525

Query: 520 SGLFLFMMLRLVAGVWRLGTKSGPW 543
            GL +FM LR+VAG  RL  + GPW
Sbjct: 526 VGLSMFMGLRMVAGFSRLMWRKGPW 549

BLAST of CSPI04G16180.2 vs. ExPASy Swiss-Prot
Match: Q9SYD6 (Protein DETOXIFICATION 42 OS=Arabidopsis thaliana OX=3702 GN=DTX42 PE=2 SV=2)

HSP 1 Score: 385.2 bits (988), Expect = 1.3e-105
Identity = 222/489 (45.40%), Postives = 312/489 (63.80%), Query Frame = 0

Query: 92  AAFKFDELALDILAIALPAALALAADPIASLIDTAFVGHIGSTELAAIGVSASVFNLVSK 151
           +  KFDEL L+I  IALPAALAL ADPIASL+DTAF+G IG  ELAA+GVS ++FN VS+
Sbjct: 25  SVLKFDELGLEIARIALPAALALTADPIASLVDTAFIGQIGPVELAAVGVSIALFNQVSR 84

Query: 152 LFNVPLLNITTSFVAEEQALIN-----ADEKNIVQTDI-DAIEETRE------------- 211
           +   PL++ITTSFVAEE A  +      D K  ++  I +  EET E             
Sbjct: 85  IAIFPLVSITTSFVAEEDACSSQQDTVRDHKECIEIGINNPTEETIELIPEKHKDSLSDE 144

Query: 212 ----------------KKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSSM 271
                           K+ + S S++L +   LG+ +AV L   +  L+  MG+  DS M
Sbjct: 145 FKTSSSIFSISKPPAKKRNIPSASSALIIGGVLGLFQAVFLISAAKPLLSFMGVKHDSPM 204

Query: 272 RAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCG 331
             P++++LSLR+ GAP ++++LAAQG FRGFKDT TPL+AT  G++ N +LDP+ IF   
Sbjct: 205 MRPSQRYLSLRSLGAPAVLLSLAAQGVFRGFKDTTTPLFATVIGDVTNIILDPIFIFVFR 264

Query: 332 FGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLV 391
            G+ GAA A VIS+YL+  +LLW+L G++     S    +  R++++G LL+ R +AV  
Sbjct: 265 LGVTGAATAHVISQYLMCGILLWKLMGQVDIFNMSTKHLQFCRFMKNGFLLLMRVIAVTF 324

Query: 392 TLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQV 451
            +TL+ S+AAREG   MA +Q+C+Q+W+A SLL D  A+AGQA+LA +F  +DY+ +   
Sbjct: 325 CVTLSASLAAREGSTSMAAFQVCLQVWLATSLLADGYAVAGQAILASAFAKKDYKRAAAT 384

Query: 452 IYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFVV 511
             R LQ+GL+ G  LA+IL  G    + +F+ D +VL     GL FVAG+QP+NALAFV 
Sbjct: 385 ASRVLQLGLVLGFVLAVILGAGLHFGARVFTKDDKVLHLISIGLPFVAGTQPINALAFVF 444

Query: 512 DGLYYGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRLG 546
           DG+ +G SDFGYAA S+V+V +VS + LL ++ T G  G+W GL ++M LR   G WR+G
Sbjct: 445 DGVNFGASDFGYAAASLVMVAIVSILCLLFLSSTHGFIGLWFGLTIYMSLRAAVGFWRIG 504

BLAST of CSPI04G16180.2 vs. ExPASy Swiss-Prot
Match: Q9SFB0 (Protein DETOXIFICATION 43 OS=Arabidopsis thaliana OX=3702 GN=DTX43 PE=1 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 8.2e-97
Identity = 205/490 (41.84%), Postives = 302/490 (61.63%), Query Frame = 0

Query: 94  FKFDELALDILAIALPAALALAADPIASLIDTAFVGHIGSTELAAIGVSASVFNLVSKLF 153
           F  D    +IL IA PAALALAADPIASLIDTAFVG +G+ +LAA+GVS ++FN  S++ 
Sbjct: 28  FSRDTTGREILGIAFPAALALAADPIASLIDTAFVGRLGAVQLAAVGVSIAIFNQASRIT 87

Query: 154 NVPLLNITTSFVAEEQAL----INADEKNIVQTDIDAIEET------------------- 213
             PL+++TTSFVAEE  +      A++ N+V  +   ++++                   
Sbjct: 88  IFPLVSLTTSFVAEEDTMEKMKEEANKANLVHAETILVQDSLEKGISSPTSNDTNQPQQP 147

Query: 214 ---------------REKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSS 273
                          +EK+ + + ST++ L   LG+ +A+ L   S  L+ +MG+  +S 
Sbjct: 148 PAPDTKSNSGNKSNKKEKRTIRTASTAMILGLILGLVQAIFLIFSSKLLLGVMGVKPNSP 207

Query: 274 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 333
           M +PA ++LS+RA GAP ++++LA QG FRGFKDTKTPL+AT   +++N VLDP+ IF  
Sbjct: 208 MLSPAHKYLSIRALGAPALLLSLAMQGIFRGFKDTKTPLFATVVADVINIVLDPIFIFVL 267

Query: 334 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVL 393
             GI GAAIA VIS+Y +  +L   L  +++    +    +  R+L++G LL+ART+AV 
Sbjct: 268 RLGIIGAAIAHVISQYFMTLILFVFLAKKVNLIPPNFGDLQFGRFLKNGLLLLARTIAVT 327

Query: 394 VTLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQ 453
              TLA +MAAR G  PMA +QIC+Q+W+  SLL D LA+AGQA+LA SF  +DY     
Sbjct: 328 FCQTLAAAMAARLGTTPMAAFQICLQVWLTSSLLNDGLAVAGQAILACSFAEKDYNKVTA 387

Query: 454 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFV 513
           V  R LQ+G + G+ L++ + LG    +G+FS D  V+     G+ F+A +QP+N+LAFV
Sbjct: 388 VASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDPAVIHLMAIGIPFIAATQPINSLAFV 447

Query: 514 VDGLYYGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRL 546
           +DG+ +G SDF Y AYSMV V  +S   ++ +  T G  G+W  L ++M LR + G+ R+
Sbjct: 448 LDGVNFGASDFAYTAYSMVGVAAISIAAVIYMAKTNGFIGIWIALTIYMALRAITGIARM 507

BLAST of CSPI04G16180.2 vs. ExPASy Swiss-Prot
Match: Q8W4G3 (Protein DETOXIFICATION 46, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX46 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 4.6e-15
Identity = 107/447 (23.94%), Postives = 193/447 (43.18%), Query Frame = 0

Query: 102 DILAIALPAALALAADPIASLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNIT 161
           +I+    PAA      P+ SLIDTA +G   S ELAA+G +  + + +   F    L++ 
Sbjct: 118 EIVMFTGPAAGLWLCGPLMSLIDTAVIGQGSSLELAALGPATVICDYLCYTF--MFLSVA 177

Query: 162 TSFVAEEQALINADEKNIVQTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLSL-G 221
           TS         N    ++ + D D ++          +S  L +    G+   V+  L G
Sbjct: 178 TS---------NLVATSLARQDKDEVQH--------QISILLFIGLACGVTMMVLTRLFG 237

Query: 222 SGTLMDIMGIPVDSSMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAG 281
           S  L    G+  ++ +   A +++ +R    P ++I   AQ    G KD+  PL A A  
Sbjct: 238 SWALTAFTGVK-NADIVPAANKYVQIRGLAWPAVLIGWVAQSASLGMKDSWGPLKALAVA 297

Query: 282 NLLNAVLDPLLIFFCGFGIGGAAIATVISEYLIAFVLLWRLN--GEISFTLSSIDGGRIA 341
           + +N V D +L  F G+GI GAA AT++S+ + A++++  LN  G  +F+        + 
Sbjct: 298 SAINGVGDVVLCTFLGYGIAGAAWATMVSQVVAAYMMMDALNKKGYSAFSFCVPSPSELL 357

Query: 342 RYLQSGGLLMARTLAVLVTLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQ 401
                   +    ++ ++  TL    A   G   +A +Q+ +QI+   ++  + L+   Q
Sbjct: 358 TIFGLAAPVFITMMSKVLFYTLLVYFATSMGTNIIAAHQVMLQIYTMSTVWGEPLSQTAQ 417

Query: 402 A----LLAGSFTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLE 461
           +    LL G    ++   +R ++   + IG   GI +  I       F G+F+ D  V  
Sbjct: 418 SFMPELLFG--INRNLPKARVLLKSLVIIGATLGIVVGTIGTAVPWLFPGIFTRDKVVTS 477

Query: 462 TARSGLLFVAGSQPVNALAFVVDGLYYGVSDFGYAAYSMV-LVGLVSSIYLLVVTPTFGL 521
                ++    +  +      ++G      D  Y + SM   + +   + +L+    FGL
Sbjct: 478 EMHKVIIPYFLALSITPSTHSLEGTLLAGRDLRYISLSMTGCLAVAGLLLMLLSNGGFGL 537

Query: 522 PGVWSGLFLFMMLRLVAGVWRLGTKSG 541
            G W  L  F   R    ++RL ++ G
Sbjct: 538 RGCWYALVGFQWARFSLSLFRLLSRDG 542

BLAST of CSPI04G16180.2 vs. ExPASy TrEMBL
Match: A0A1S3CL06 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103502163 PE=3 SV=1)

HSP 1 Score: 979.9 bits (2532), Expect = 4.3e-282
Identity = 528/554 (95.31%), Postives = 538/554 (97.11%), Query Frame = 0

Query: 1   MATGLSVYAPSFNTHTNLSFKCQMLRRNANSGLRLPYFPKPSFHRNLTTSSLKSPLEESK 60
           MATGLS+YAPSFNTHTNLSFKCQMLRRNAN GL  PY PKPSFH+NLTTSSLKSPLEESK
Sbjct: 1   MATGLSIYAPSFNTHTNLSFKCQMLRRNANCGLLFPYLPKPSFHKNLTTSSLKSPLEESK 60

Query: 61  STPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA 120
           ST SSDQV  NR D NSS SFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA
Sbjct: 61  STASSDQVRRNRLDKNSSNSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA 120

Query: 121 SLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINADEKNIV 180
           SLIDTAFVGHIGSTELAA+GVSASVFNLVSKLFNVPLLNITTSFVAEEQALIN DEKNIV
Sbjct: 121 SLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINTDEKNIV 180

Query: 181 QTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA 240
           QTDID IE+T+EKKLLSSVSTSLALAT LGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA
Sbjct: 181 QTDIDDIEDTQEKKLLSSVSTSLALATCLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA 240

Query: 241 EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCGFGIG 300
           EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGN LNA+LDPLLIFFCGFGIG
Sbjct: 241 EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNFLNAILDPLLIFFCGFGIG 300

Query: 301 GAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL 360
           GAAIATVISEYLIAF+LLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL
Sbjct: 301 GAAIATVISEYLIAFILLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL 360

Query: 361 ATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQVIYRT 420
           ATSMAAREGPVPMAGYQI VQIWMAISLLTDALALAGQALLAGSFTLQDY+HSRQVIYRT
Sbjct: 361 ATSMAAREGPVPMAGYQISVQIWMAISLLTDALALAGQALLAGSFTLQDYKHSRQVIYRT 420

Query: 421 LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFVVDGLY 480
           LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSG LFVAGSQPVNALAFVVDGLY
Sbjct: 421 LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGSLFVAGSQPVNALAFVVDGLY 480

Query: 481 YGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRLGTKSG 540
           YGVSDFGYAAYSMVLVGLVSSI+LLVVTPTFGLPGVWSGLFLFMMLRLVAG+WRLGTKSG
Sbjct: 481 YGVSDFGYAAYSMVLVGLVSSIFLLVVTPTFGLPGVWSGLFLFMMLRLVAGIWRLGTKSG 540

Query: 541 PWELVFNEVDGKSD 555
           PWELVFNEVDGKSD
Sbjct: 541 PWELVFNEVDGKSD 554

BLAST of CSPI04G16180.2 vs. ExPASy TrEMBL
Match: A0A5D3CIE7 (Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold107G00260 PE=3 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 7.1e-269
Identity = 509/537 (94.79%), Postives = 519/537 (96.65%), Query Frame = 0

Query: 1   MATGLSVYAPSFNTHTNLSFKCQMLRRNANSGLRLPYFPKPSFHRNLTTSSLKSPLEESK 60
           MATGLS+YAPSFNTHTNLSFKCQMLRRNAN GL  PY PKPSFH+NLTTSSLKSPLEESK
Sbjct: 1   MATGLSIYAPSFNTHTNLSFKCQMLRRNANCGLLFPYLPKPSFHKNLTTSSLKSPLEESK 60

Query: 61  STPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA 120
           ST SSDQV  NR D NSS SFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA
Sbjct: 61  STASSDQVRRNRLDKNSSNSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA 120

Query: 121 SLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINADEKNIV 180
           SLIDTAFVGHIGSTELAA+GVSASVFNLVSKLFNVPLLNITTSFVAEEQALIN DEKNIV
Sbjct: 121 SLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINTDEKNIV 180

Query: 181 QTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA 240
           QTDID IE+T+EKKLLSSVSTSLALAT LGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA
Sbjct: 181 QTDIDDIEDTQEKKLLSSVSTSLALATCLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA 240

Query: 241 EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCGFGIG 300
           EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNA+LDPLLIFFCGFGIG
Sbjct: 241 EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAILDPLLIFFCGFGIG 300

Query: 301 GAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL 360
           GAAIATVISEYLIAF+LLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL
Sbjct: 301 GAAIATVISEYLIAFILLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL 360

Query: 361 ATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQVIYRT 420
           ATSMAAREGPVPMAGYQI VQIWMAISLLTDALALAGQALLAGSFTLQDY+HSRQVIYRT
Sbjct: 361 ATSMAAREGPVPMAGYQISVQIWMAISLLTDALALAGQALLAGSFTLQDYKHSRQVIYRT 420

Query: 421 LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFVVDGLY 480
           LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSG LFVAGSQPVNALAFVVDGLY
Sbjct: 421 LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGSLFVAGSQPVNALAFVVDGLY 480

Query: 481 YGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRLGT 538
           YGVSDFGYAAYSMVLVGLVSSI+LLVVTPTFGLPGVWSGLFLFMMLRLVAG+W   T
Sbjct: 481 YGVSDFGYAAYSMVLVGLVSSIFLLVVTPTFGLPGVWSGLFLFMMLRLVAGIWSENT 537

BLAST of CSPI04G16180.2 vs. ExPASy TrEMBL
Match: A0A6J1GD47 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111453089 PE=3 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 1.1e-258
Identity = 491/559 (87.84%), Postives = 515/559 (92.13%), Query Frame = 0

Query: 1   MATGLSVYAPSFNTHTNLSFKCQMLRRNANSGLRLPYFPKPSFHRNLTTSSLKSPLEESK 60
           MATGLS+Y P FNT TNLS KCQ+LRRNAN  +R  YF K SF +NLTTSSLKSP EE K
Sbjct: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STPSSDQVPPNRPDNNSSKSFLSASV-----NRFRDAAFKFDELALDILAIALPAALALA 120
           ST SS+Q+  N P   SS S LSASV     NRFRDA FKFD+LALDILAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSASVSVPLLNRFRDADFKFDQLALDILAIALPAALALA 120

Query: 121 ADPIASLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINAD 180
           ADPIASLIDTAFVGHIGSTELAA+GVSASVFNLVSKLFNVPLLNITTSFVAEEQALI+ +
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHTN 180

Query: 181 EKNIVQTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSS 240
           EKN  Q +ID IEE + KKLLSSVSTSLALA GLGIAEAVMLSLGSG+LMDIMGIPV SS
Sbjct: 181 EKNTAQINIDGIEENQGKKLLSSVSTSLALAAGLGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300
           MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGR+ARYL+SGGLLM RTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRVARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQ 420
           VTLTLATSMAAREG VPMAGYQICVQIW+AISLLTDALALAGQALLA S+TLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFV 480
           VIYRTLQIGLISGISL+IILFLGFGAFSGLFSADAEVLE ARSGL FVAGSQPVNALAFV
Sbjct: 421 VIYRTLQIGLISGISLSIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVNALAFV 480

Query: 481 VDGLYYGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRL 540
           VDGLYYGVSDFGYAAYSMVLVGL+SS+YLLVVTP FGLPGVWSGLFLFMMLRL+AG+WRL
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540

Query: 541 GTKSGPWELVFNEVDGKSD 555
           GTKSGPWE+VFNE+D KSD
Sbjct: 541 GTKSGPWEMVFNELDEKSD 559

BLAST of CSPI04G16180.2 vs. ExPASy TrEMBL
Match: A0A6J1IP82 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111478206 PE=3 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 6.2e-257
Identity = 489/559 (87.48%), Postives = 513/559 (91.77%), Query Frame = 0

Query: 1   MATGLSVYAPSFNTHTNLSFKCQMLRRNANSGLRLPYFPKPSFHRNLTTSSLKSPLEESK 60
           MATGLS+Y P FNT TNLS KCQ+LRRNAN  +R  YF K SF +NLTTSSLKSP EE K
Sbjct: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STPSSDQVPPNRPDNNSSKSFLSASV-----NRFRDAAFKFDELALDILAIALPAALALA 120
           ST SS+Q+  N P   SS S LS  V     NRFRDA FKFD+LALDILAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120

Query: 121 ADPIASLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINAD 180
           ADPIASLIDTAFVGHIGSTELAA+GVSASVFNLVSKLFNVPLLNITTSFVAEEQALI+ +
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN 180

Query: 181 EKNIVQTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSS 240
           EKN VQ +ID IEE + KKLLSSVSTSLALA G+GIAEAVMLSLGSG+LMDIMGIPV SS
Sbjct: 181 EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300
           MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEIS TLSSIDGGRIARYL+SGGLLM RTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQ 420
           VTLTLATSMAAREG VPMAGYQICVQIW+AISLLTDALALAGQALLA S+TLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFV 480
           VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLE ARSGL FVAGSQPV+ALAFV
Sbjct: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV 480

Query: 481 VDGLYYGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRL 540
           VDGLYYGVSDFGYAAYSMVLVGL+SS+YLLVVTP FGLPGVWSGLFLFMMLRL+AG+WRL
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540

Query: 541 GTKSGPWELVFNEVDGKSD 555
           GTKSGPWE+VFNE+D KSD
Sbjct: 541 GTKSGPWEMVFNELDEKSD 559

BLAST of CSPI04G16180.2 vs. ExPASy TrEMBL
Match: A0A5A7T830 (Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold141G00090 PE=3 SV=1)

HSP 1 Score: 880.2 bits (2273), Expect = 4.6e-252
Identity = 489/538 (90.89%), Postives = 498/538 (92.57%), Query Frame = 0

Query: 1   MATGLSVYAPSFNTHTNLSFKCQMLRRNANSGLRLPYFPKPSFHRNLTTSSLKSPLEESK 60
           MATGLS+YAPSFNTHTNLSFKCQMLRRNAN GL  PY PKPSFH+NLTTSSLKSPLEESK
Sbjct: 1   MATGLSIYAPSFNTHTNLSFKCQMLRRNANCGLLFPYLPKPSFHKNLTTSSLKSPLEESK 60

Query: 61  STPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA 120
           ST SSDQV  NR D NSS SFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA
Sbjct: 61  STASSDQVRRNRLDKNSSNSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA 120

Query: 121 SLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINADEKNIV 180
           SLIDTAFVGHIGSTELAA+GVSASVFNLVSKLFNVPLLNITTSFVAEEQALIN DEKNIV
Sbjct: 121 SLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINTDEKNIV 180

Query: 181 QTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA 240
           QTDID IE+T+EKKLLSSVSTSLALAT LGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA
Sbjct: 181 QTDIDDIEDTQEKKLLSSVSTSLALATCLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA 240

Query: 241 EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCGFGIG 300
           EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGN LNA+LDPLLIFFCGFGIG
Sbjct: 241 EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNFLNAILDPLLIFFCGFGIG 300

Query: 301 GAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL 360
           GAAIATVISEYLIAF+LLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL
Sbjct: 301 GAAIATVISEYLIAFILLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL 360

Query: 361 ATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQA-LLAGSFTLQDYEHSRQVIYR 420
           ATSMAAREGPVPMAGYQI VQIWMAISLLTDALALAGQ        T   Y H       
Sbjct: 361 ATSMAAREGPVPMAGYQISVQIWMAISLLTDALALAGQVQRFPPKLT---YIHYSAKPLT 420

Query: 421 TLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFVVDGL 480
            L IGLISGISLAIILFLGFGAFSGLFSADAEVLETARSG LFVAGSQPVNALAFVVDGL
Sbjct: 421 FLLIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGSLFVAGSQPVNALAFVVDGL 480

Query: 481 YYGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRLGT 538
           YYGVSDFGYAAYSMVLVGLVSSI+LLVVTPTFGLPGVWSGLFLFMMLRLVAG+W   T
Sbjct: 481 YYGVSDFGYAAYSMVLVGLVSSIFLLVVTPTFGLPGVWSGLFLFMMLRLVAGIWSENT 535

BLAST of CSPI04G16180.2 vs. NCBI nr
Match: XP_031739706.1 (protein DETOXIFICATION 44, chloroplastic isoform X1 [Cucumis sativus] >XP_031739707.1 protein DETOXIFICATION 44, chloroplastic isoform X1 [Cucumis sativus] >XP_031740771.1 protein DETOXIFICATION 44, chloroplastic isoform X1 [Cucumis sativus] >XP_031740772.1 protein DETOXIFICATION 44, chloroplastic isoform X1 [Cucumis sativus] >KAE8649578.1 hypothetical protein Csa_021546 [Cucumis sativus] >KAE8649582.1 hypothetical protein Csa_012793 [Cucumis sativus])

HSP 1 Score: 1030.4 bits (2663), Expect = 5.7e-297
Identity = 554/554 (100.00%), Postives = 554/554 (100.00%), Query Frame = 0

Query: 1   MATGLSVYAPSFNTHTNLSFKCQMLRRNANSGLRLPYFPKPSFHRNLTTSSLKSPLEESK 60
           MATGLSVYAPSFNTHTNLSFKCQMLRRNANSGLRLPYFPKPSFHRNLTTSSLKSPLEESK
Sbjct: 1   MATGLSVYAPSFNTHTNLSFKCQMLRRNANSGLRLPYFPKPSFHRNLTTSSLKSPLEESK 60

Query: 61  STPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA 120
           STPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA
Sbjct: 61  STPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA 120

Query: 121 SLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINADEKNIV 180
           SLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINADEKNIV
Sbjct: 121 SLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINADEKNIV 180

Query: 181 QTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA 240
           QTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA
Sbjct: 181 QTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA 240

Query: 241 EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCGFGIG 300
           EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCGFGIG
Sbjct: 241 EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCGFGIG 300

Query: 301 GAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL 360
           GAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL
Sbjct: 301 GAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL 360

Query: 361 ATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQVIYRT 420
           ATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQVIYRT
Sbjct: 361 ATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQVIYRT 420

Query: 421 LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFVVDGLY 480
           LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFVVDGLY
Sbjct: 421 LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFVVDGLY 480

Query: 481 YGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRLGTKSG 540
           YGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRLGTKSG
Sbjct: 481 YGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRLGTKSG 540

Query: 541 PWELVFNEVDGKSD 555
           PWELVFNEVDGKSD
Sbjct: 541 PWELVFNEVDGKSD 554

BLAST of CSPI04G16180.2 vs. NCBI nr
Match: XP_008464231.1 (PREDICTED: protein DETOXIFICATION 44, chloroplastic isoform X1 [Cucumis melo])

HSP 1 Score: 979.9 bits (2532), Expect = 8.8e-282
Identity = 528/554 (95.31%), Postives = 538/554 (97.11%), Query Frame = 0

Query: 1   MATGLSVYAPSFNTHTNLSFKCQMLRRNANSGLRLPYFPKPSFHRNLTTSSLKSPLEESK 60
           MATGLS+YAPSFNTHTNLSFKCQMLRRNAN GL  PY PKPSFH+NLTTSSLKSPLEESK
Sbjct: 1   MATGLSIYAPSFNTHTNLSFKCQMLRRNANCGLLFPYLPKPSFHKNLTTSSLKSPLEESK 60

Query: 61  STPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA 120
           ST SSDQV  NR D NSS SFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA
Sbjct: 61  STASSDQVRRNRLDKNSSNSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA 120

Query: 121 SLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINADEKNIV 180
           SLIDTAFVGHIGSTELAA+GVSASVFNLVSKLFNVPLLNITTSFVAEEQALIN DEKNIV
Sbjct: 121 SLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINTDEKNIV 180

Query: 181 QTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA 240
           QTDID IE+T+EKKLLSSVSTSLALAT LGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA
Sbjct: 181 QTDIDDIEDTQEKKLLSSVSTSLALATCLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA 240

Query: 241 EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCGFGIG 300
           EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGN LNA+LDPLLIFFCGFGIG
Sbjct: 241 EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNFLNAILDPLLIFFCGFGIG 300

Query: 301 GAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL 360
           GAAIATVISEYLIAF+LLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL
Sbjct: 301 GAAIATVISEYLIAFILLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL 360

Query: 361 ATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQVIYRT 420
           ATSMAAREGPVPMAGYQI VQIWMAISLLTDALALAGQALLAGSFTLQDY+HSRQVIYRT
Sbjct: 361 ATSMAAREGPVPMAGYQISVQIWMAISLLTDALALAGQALLAGSFTLQDYKHSRQVIYRT 420

Query: 421 LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFVVDGLY 480
           LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSG LFVAGSQPVNALAFVVDGLY
Sbjct: 421 LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGSLFVAGSQPVNALAFVVDGLY 480

Query: 481 YGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRLGTKSG 540
           YGVSDFGYAAYSMVLVGLVSSI+LLVVTPTFGLPGVWSGLFLFMMLRLVAG+WRLGTKSG
Sbjct: 481 YGVSDFGYAAYSMVLVGLVSSIFLLVVTPTFGLPGVWSGLFLFMMLRLVAGIWRLGTKSG 540

Query: 541 PWELVFNEVDGKSD 555
           PWELVFNEVDGKSD
Sbjct: 541 PWELVFNEVDGKSD 554

BLAST of CSPI04G16180.2 vs. NCBI nr
Match: TYK10944.1 (protein DETOXIFICATION 44 [Cucumis melo var. makuwa])

HSP 1 Score: 936.0 bits (2418), Expect = 1.5e-268
Identity = 509/537 (94.79%), Postives = 519/537 (96.65%), Query Frame = 0

Query: 1   MATGLSVYAPSFNTHTNLSFKCQMLRRNANSGLRLPYFPKPSFHRNLTTSSLKSPLEESK 60
           MATGLS+YAPSFNTHTNLSFKCQMLRRNAN GL  PY PKPSFH+NLTTSSLKSPLEESK
Sbjct: 1   MATGLSIYAPSFNTHTNLSFKCQMLRRNANCGLLFPYLPKPSFHKNLTTSSLKSPLEESK 60

Query: 61  STPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA 120
           ST SSDQV  NR D NSS SFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA
Sbjct: 61  STASSDQVRRNRLDKNSSNSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA 120

Query: 121 SLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINADEKNIV 180
           SLIDTAFVGHIGSTELAA+GVSASVFNLVSKLFNVPLLNITTSFVAEEQALIN DEKNIV
Sbjct: 121 SLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINTDEKNIV 180

Query: 181 QTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA 240
           QTDID IE+T+EKKLLSSVSTSLALAT LGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA
Sbjct: 181 QTDIDDIEDTQEKKLLSSVSTSLALATCLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA 240

Query: 241 EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCGFGIG 300
           EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNA+LDPLLIFFCGFGIG
Sbjct: 241 EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAILDPLLIFFCGFGIG 300

Query: 301 GAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL 360
           GAAIATVISEYLIAF+LLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL
Sbjct: 301 GAAIATVISEYLIAFILLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL 360

Query: 361 ATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQVIYRT 420
           ATSMAAREGPVPMAGYQI VQIWMAISLLTDALALAGQALLAGSFTLQDY+HSRQVIYRT
Sbjct: 361 ATSMAAREGPVPMAGYQISVQIWMAISLLTDALALAGQALLAGSFTLQDYKHSRQVIYRT 420

Query: 421 LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFVVDGLY 480
           LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSG LFVAGSQPVNALAFVVDGLY
Sbjct: 421 LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGSLFVAGSQPVNALAFVVDGLY 480

Query: 481 YGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRLGT 538
           YGVSDFGYAAYSMVLVGLVSSI+LLVVTPTFGLPGVWSGLFLFMMLRLVAG+W   T
Sbjct: 481 YGVSDFGYAAYSMVLVGLVSSIFLLVVTPTFGLPGVWSGLFLFMMLRLVAGIWSENT 537

BLAST of CSPI04G16180.2 vs. NCBI nr
Match: XP_038883441.1 (protein DETOXIFICATION 44, chloroplastic [Benincasa hispida])

HSP 1 Score: 912.9 bits (2358), Expect = 1.3e-261
Identity = 500/560 (89.29%), Postives = 520/560 (92.86%), Query Frame = 0

Query: 1   MATGLSVYAPSFNTHTNLSFKCQMLRRNANSGLRLPYFPKPSFHRNLTTSSLKSPLEESK 60
           MATGLS+Y P FNT TNLS KCQMLRR AN  +R   FPK SF +NLTTSSLKSP EE K
Sbjct: 1   MATGLSIYVPCFNTDTNLSSKCQMLRRIANCEVRFRDFPKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STPSSDQVPPNRPDNNSSKSFLSASV-----NRFRDAAFKFDELALDILAIALPAALALA 120
           ST SS+QV  NR D  SS SFLS SV     NRFRDA FKFD+LALDILAIALPAALALA
Sbjct: 61  STASSNQVRRNRLDKKSSNSFLSKSVSVPLLNRFRDAGFKFDKLALDILAIALPAALALA 120

Query: 121 ADPIASLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINAD 180
           ADPIASLIDTAFVGHIGSTELAA+GVSASVFNLVSKLFNVPLLNITTSFVAEEQALIN +
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINTN 180

Query: 181 EKNIVQTDIDAI-EETREKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDS 240
           EKN+VQ +ID I EE +EKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDS
Sbjct: 181 EKNVVQLNIDGIEEENQEKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDS 240

Query: 241 SMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFF 300
           SMRAPAEQFLSLRAFGAPPIV+ALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFF
Sbjct: 241 SMRAPAEQFLSLRAFGAPPIVLALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFF 300

Query: 301 CGFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAV 360
           CGFGIGGAAIATVI+EYL+A VL+WRL+GEISFTLSSIDG RIARYLQSGGLLM RTLAV
Sbjct: 301 CGFGIGGAAIATVITEYLVASVLVWRLSGEISFTLSSIDGSRIARYLQSGGLLMGRTLAV 360

Query: 361 LVTLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSR 420
           LVTLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLA S+TLQDYEHSR
Sbjct: 361 LVTLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLASSYTLQDYEHSR 420

Query: 421 QVIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAF 480
           QVIYRTLQIGL+SGISLAIILFLGFGAFSGLFS+DAEVLE ARSGLLFVAGSQPVNALAF
Sbjct: 421 QVIYRTLQIGLVSGISLAIILFLGFGAFSGLFSSDAEVLEIARSGLLFVAGSQPVNALAF 480

Query: 481 VVDGLYYGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWR 540
           VVDGLYYGVSDFGYAAYSMVLVGLVSSIYLLVVTP  GLPGVWSGLFLFMMLRLVAG+WR
Sbjct: 481 VVDGLYYGVSDFGYAAYSMVLVGLVSSIYLLVVTPALGLPGVWSGLFLFMMLRLVAGIWR 540

Query: 541 LGTKSGPWELVFNEVDGKSD 555
           LGTKSGPWE+VFNEVDGKSD
Sbjct: 541 LGTKSGPWEIVFNEVDGKSD 560

BLAST of CSPI04G16180.2 vs. NCBI nr
Match: XP_031739709.1 (protein DETOXIFICATION 44, chloroplastic isoform X3 [Cucumis sativus] >XP_031740774.1 protein DETOXIFICATION 44, chloroplastic isoform X3 [Cucumis sativus])

HSP 1 Score: 910.6 bits (2352), Expect = 6.6e-261
Identity = 493/494 (99.80%), Postives = 494/494 (100.00%), Query Frame = 0

Query: 1   MATGLSVYAPSFNTHTNLSFKCQMLRRNANSGLRLPYFPKPSFHRNLTTSSLKSPLEESK 60
           MATGLSVYAPSFNTHTNLSFKCQMLRRNANSGLRLPYFPKPSFHRNLTTSSLKSPLEESK
Sbjct: 1   MATGLSVYAPSFNTHTNLSFKCQMLRRNANSGLRLPYFPKPSFHRNLTTSSLKSPLEESK 60

Query: 61  STPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA 120
           STPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA
Sbjct: 61  STPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDELALDILAIALPAALALAADPIA 120

Query: 121 SLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINADEKNIV 180
           SLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINADEKNIV
Sbjct: 121 SLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINADEKNIV 180

Query: 181 QTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA 240
           QTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA
Sbjct: 181 QTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSSMRAPA 240

Query: 241 EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCGFGIG 300
           EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCGFGIG
Sbjct: 241 EQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCGFGIG 300

Query: 301 GAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL 360
           GAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL
Sbjct: 301 GAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLVTLTL 360

Query: 361 ATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQVIYRT 420
           ATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQVIYRT
Sbjct: 361 ATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQVIYRT 420

Query: 421 LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFVVDGLY 480
           LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFVVDGLY
Sbjct: 421 LQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFVVDGLY 480

Query: 481 YGVSDFGYAAYSMV 495
           YGVSDFGYAAYSM+
Sbjct: 481 YGVSDFGYAAYSMI 494

BLAST of CSPI04G16180.2 vs. TAIR 10
Match: AT2G38330.1 (MATE efflux family protein )

HSP 1 Score: 566.6 bits (1459), Expect = 2.2e-161
Identity = 309/509 (60.71%), Postives = 387/509 (76.03%), Query Frame = 0

Query: 39  PKPSFHRNLTTSSLKSPLEESKSTPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDE 98
           P  S  R +   S  SP +ES +  +S Q    RP+   +        +          +
Sbjct: 22  PNSSIRRTIVCKS--SPRDESPAVSTSSQ----RPEKQQNPLTSQNKPDHDHKPDPGIGK 81

Query: 99  LALDILAIALPAALALAADPIASLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLL 158
           + ++I++IALPAALALAADPI SL+DTAFVGHIGS ELAA+GVS SVFNLVSKLFNVPLL
Sbjct: 82  IGMEIMSIALPAALALAADPITSLVDTAFVGHIGSAELAAVGVSVSVFNLVSKLFNVPLL 141

Query: 159 NITTSFVAEEQALINADEKNIVQTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVMLS 218
           N+TTSFVAEEQA+   D+ + ++T          KK+L SVSTSL LA G+GIAEA+ LS
Sbjct: 142 NVTTSFVAEEQAIAAKDDNDSIET---------SKKVLPSVSTSLVLAAGVGIAEAIALS 201

Query: 219 LGSGTLMDIMGIPVDSSMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATA 278
           LGS  LMD+M IP DS MR PAEQFL LRA+GAPPIV+ALAAQG FRGFKDT TPLYA  
Sbjct: 202 LGSDFLMDVMAIPFDSPMRIPAEQFLRLRAYGAPPIVVALAAQGAFRGFKDTTTPLYAVV 261

Query: 279 AGNLLNAVLDPLLIFFCGFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIA 338
           AGN+LNAVLDP+LIF  GFGI GAA ATVISEYLIAF+LLW+LN  +      I  GR  
Sbjct: 262 AGNVLNAVLDPILIFVLGFGISGAAAATVISEYLIAFILLWKLNENVVLLSPQIKVGRAN 321

Query: 339 RYLQSGGLLMARTLAVLVTLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQ 398
           +YL+SGGLL+ RT+A+LV  TLATS+AA+ GP  MAG+QI ++IW+A+SLLTDALA+A Q
Sbjct: 322 QYLKSGGLLIGRTVALLVPFTLATSLAAQNGPTQMAGHQIVLEIWLAVSLLTDALAIAAQ 381

Query: 399 ALLAGSFTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARS 458
           +LLA +++  +Y+ +R+V++  LQ+GL +G  LA +LF+ F  FS LF+ D+EVL+ A S
Sbjct: 382 SLLATTYSQGEYKQAREVLFGVLQVGLATGTGLAAVLFITFEPFSSLFTTDSEVLKIALS 441

Query: 459 GLLFVAGSQPVNALAFVVDGLYYGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWS 518
           G LFVAGSQPVNALAFV+DGLYYGVSDFG+AAYSMV+VG +SS+++LV  PTFGL G+W+
Sbjct: 442 GTLFVAGSQPVNALAFVLDGLYYGVSDFGFAAYSMVIVGFISSLFMLVAAPTFGLAGIWT 501

Query: 519 GLFLFMMLRLVAGVWRLGTKSGPWELVFN 548
           GLFLFM LRLVAG WRLGT++GPW+++++
Sbjct: 502 GLFLFMALRLVAGAWRLGTRTGPWKMLWS 515

BLAST of CSPI04G16180.2 vs. TAIR 10
Match: AT4G38380.1 (MATE efflux family protein )

HSP 1 Score: 399.4 bits (1025), Expect = 4.6e-111
Identity = 237/505 (46.93%), Postives = 329/505 (65.15%), Query Frame = 0

Query: 40  KPSFHRNLTTSSLKSPLEESKSTPSSDQVPPNRPDNNSSKSFLSASVNRFRDAAFKFDEL 99
           K S H N           +S++ P    V     D++ S       VN       +  ++
Sbjct: 46  KCSLHTNPNPMFPFVTRRKSQTNPDCGVVKLGEEDDSCSSLDKLPEVNGVHTGVARPVDI 105

Query: 100 ALDILAIALPAALALAADPIASLIDTAFVGHIGSTELAAIGVSASVFNLVSKLFNVPLLN 159
             +++ ++LPA    A DP+  L++TA++G +GS EL + GVS ++FN +SKLFN+PLL+
Sbjct: 106 KRELVMLSLPAIAGQAIDPLTLLMETAYIGRLGSVELGSAGVSMAIFNTISKLFNIPLLS 165

Query: 160 ITTSFVAEEQALINADE--KNIVQTDIDAIEETREKKLLSSVSTSLALATGLGIAEAVML 219
           + TSFVAE+ A I A +      Q+DI + +   E+K LSSVST+L LA G+GI EA+ L
Sbjct: 166 VATSFVAEDIAKIAAQDLASEDSQSDIPS-QGLPERKQLSSVSTALVLAIGIGIFEALAL 225

Query: 220 SLGSGTLMDIMGIPVDSSMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYAT 279
           SL SG  + +MGI   S M  PA QFL LRA GAP  V++LA QG FRGFKDTKTP+Y  
Sbjct: 226 SLASGPFLRLMGIQSMSEMFIPARQFLVLRALGAPAYVVSLALQGIFRGFKDTKTPVYCL 285

Query: 280 AAGNLLNAVLDPLLIFFCGFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRI 339
             GN L   L PL I+    G+ GAAI++VIS+Y +A ++L  LN  +      I   + 
Sbjct: 286 GIGNFLAVFLFPLFIYKFRMGVAGAAISSVISQYTVAILMLILLNKRVILLPPKIGSLKF 345

Query: 340 ARYLQSGGLLMARTLAVLVTLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAG 399
             YL+SGG ++ RTL+VLVT+T+ATSMAAR+G   MA +QIC+Q+W+A+SLLTDALA +G
Sbjct: 346 GDYLKSGGFVLGRTLSVLVTMTVATSMAARQGVFAMAAHQICMQVWLAVSLLTDALASSG 405

Query: 400 QALLAGSFTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETAR 459
           QAL+A S + +D+E  ++V    L+IG+++GI+LAI+L + F + +GLFS D EVL   R
Sbjct: 406 QALIASSASKRDFEGVKEVTTFVLKIGVVTGIALAIVLGMSFSSIAGLFSKDPEVLRIVR 465

Query: 460 SGLLFVAGSQPVNALAFVVDGLYYGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVW 519
            G+LFVA +QP+ ALAF+ DGL+YG+SDF YAA SM++VG +SS ++L      GL GVW
Sbjct: 466 KGVLFVAATQPITALAFIFDGLHYGMSDFPYAACSMMVVGGISSAFMLYAPAGLGLSGVW 525

Query: 520 SGLFLFMMLRLVAGVWRLGTKSGPW 543
            GL +FM LR+VAG  RL  + GPW
Sbjct: 526 VGLSMFMGLRMVAGFSRLMWRKGPW 549

BLAST of CSPI04G16180.2 vs. TAIR 10
Match: AT1G51340.2 (MATE efflux family protein )

HSP 1 Score: 385.2 bits (988), Expect = 9.0e-107
Identity = 222/489 (45.40%), Postives = 312/489 (63.80%), Query Frame = 0

Query: 92  AAFKFDELALDILAIALPAALALAADPIASLIDTAFVGHIGSTELAAIGVSASVFNLVSK 151
           +  KFDEL L+I  IALPAALAL ADPIASL+DTAF+G IG  ELAA+GVS ++FN VS+
Sbjct: 25  SVLKFDELGLEIARIALPAALALTADPIASLVDTAFIGQIGPVELAAVGVSIALFNQVSR 84

Query: 152 LFNVPLLNITTSFVAEEQALIN-----ADEKNIVQTDI-DAIEETRE------------- 211
           +   PL++ITTSFVAEE A  +      D K  ++  I +  EET E             
Sbjct: 85  IAIFPLVSITTSFVAEEDACSSQQDTVRDHKECIEIGINNPTEETIELIPEKHKDSLSDE 144

Query: 212 ----------------KKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSSM 271
                           K+ + S S++L +   LG+ +AV L   +  L+  MG+  DS M
Sbjct: 145 FKTSSSIFSISKPPAKKRNIPSASSALIIGGVLGLFQAVFLISAAKPLLSFMGVKHDSPM 204

Query: 272 RAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCG 331
             P++++LSLR+ GAP ++++LAAQG FRGFKDT TPL+AT  G++ N +LDP+ IF   
Sbjct: 205 MRPSQRYLSLRSLGAPAVLLSLAAQGVFRGFKDTTTPLFATVIGDVTNIILDPIFIFVFR 264

Query: 332 FGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLV 391
            G+ GAA A VIS+YL+  +LLW+L G++     S    +  R++++G LL+ R +AV  
Sbjct: 265 LGVTGAATAHVISQYLMCGILLWKLMGQVDIFNMSTKHLQFCRFMKNGFLLLMRVIAVTF 324

Query: 392 TLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQV 451
            +TL+ S+AAREG   MA +Q+C+Q+W+A SLL D  A+AGQA+LA +F  +DY+ +   
Sbjct: 325 CVTLSASLAAREGSTSMAAFQVCLQVWLATSLLADGYAVAGQAILASAFAKKDYKRAAAT 384

Query: 452 IYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFVV 511
             R LQ+GL+ G  LA+IL  G    + +F+ D +VL     GL FVAG+QP+NALAFV 
Sbjct: 385 ASRVLQLGLVLGFVLAVILGAGLHFGARVFTKDDKVLHLISIGLPFVAGTQPINALAFVF 444

Query: 512 DGLYYGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRLG 546
           DG+ +G SDFGYAA S+V+V +VS + LL ++ T G  G+W GL ++M LR   G WR+G
Sbjct: 445 DGVNFGASDFGYAAASLVMVAIVSILCLLFLSSTHGFIGLWFGLTIYMSLRAAVGFWRIG 504

BLAST of CSPI04G16180.2 vs. TAIR 10
Match: AT1G51340.1 (MATE efflux family protein )

HSP 1 Score: 385.2 bits (988), Expect = 9.0e-107
Identity = 222/489 (45.40%), Postives = 312/489 (63.80%), Query Frame = 0

Query: 92  AAFKFDELALDILAIALPAALALAADPIASLIDTAFVGHIGSTELAAIGVSASVFNLVSK 151
           +  KFDEL L+I  IALPAALAL ADPIASL+DTAF+G IG  ELAA+GVS ++FN VS+
Sbjct: 19  SVLKFDELGLEIARIALPAALALTADPIASLVDTAFIGQIGPVELAAVGVSIALFNQVSR 78

Query: 152 LFNVPLLNITTSFVAEEQALIN-----ADEKNIVQTDI-DAIEETRE------------- 211
           +   PL++ITTSFVAEE A  +      D K  ++  I +  EET E             
Sbjct: 79  IAIFPLVSITTSFVAEEDACSSQQDTVRDHKECIEIGINNPTEETIELIPEKHKDSLSDE 138

Query: 212 ----------------KKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSSM 271
                           K+ + S S++L +   LG+ +AV L   +  L+  MG+  DS M
Sbjct: 139 FKTSSSIFSISKPPAKKRNIPSASSALIIGGVLGLFQAVFLISAAKPLLSFMGVKHDSPM 198

Query: 272 RAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCG 331
             P++++LSLR+ GAP ++++LAAQG FRGFKDT TPL+AT  G++ N +LDP+ IF   
Sbjct: 199 MRPSQRYLSLRSLGAPAVLLSLAAQGVFRGFKDTTTPLFATVIGDVTNIILDPIFIFVFR 258

Query: 332 FGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVLV 391
            G+ GAA A VIS+YL+  +LLW+L G++     S    +  R++++G LL+ R +AV  
Sbjct: 259 LGVTGAATAHVISQYLMCGILLWKLMGQVDIFNMSTKHLQFCRFMKNGFLLLMRVIAVTF 318

Query: 392 TLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQV 451
            +TL+ S+AAREG   MA +Q+C+Q+W+A SLL D  A+AGQA+LA +F  +DY+ +   
Sbjct: 319 CVTLSASLAAREGSTSMAAFQVCLQVWLATSLLADGYAVAGQAILASAFAKKDYKRAAAT 378

Query: 452 IYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFVV 511
             R LQ+GL+ G  LA+IL  G    + +F+ D +VL     GL FVAG+QP+NALAFV 
Sbjct: 379 ASRVLQLGLVLGFVLAVILGAGLHFGARVFTKDDKVLHLISIGLPFVAGTQPINALAFVF 438

Query: 512 DGLYYGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRLG 546
           DG+ +G SDFGYAA S+V+V +VS + LL ++ T G  G+W GL ++M LR   G WR+G
Sbjct: 439 DGVNFGASDFGYAAASLVMVAIVSILCLLFLSSTHGFIGLWFGLTIYMSLRAAVGFWRIG 498

BLAST of CSPI04G16180.2 vs. TAIR 10
Match: AT3G08040.1 (MATE efflux family protein )

HSP 1 Score: 355.9 bits (912), Expect = 5.8e-98
Identity = 205/490 (41.84%), Postives = 302/490 (61.63%), Query Frame = 0

Query: 94  FKFDELALDILAIALPAALALAADPIASLIDTAFVGHIGSTELAAIGVSASVFNLVSKLF 153
           F  D    +IL IA PAALALAADPIASLIDTAFVG +G+ +LAA+GVS ++FN  S++ 
Sbjct: 28  FSRDTTGREILGIAFPAALALAADPIASLIDTAFVGRLGAVQLAAVGVSIAIFNQASRIT 87

Query: 154 NVPLLNITTSFVAEEQAL----INADEKNIVQTDIDAIEET------------------- 213
             PL+++TTSFVAEE  +      A++ N+V  +   ++++                   
Sbjct: 88  IFPLVSLTTSFVAEEDTMEKMKEEANKANLVHAETILVQDSLEKGISSPTSNDTNQPQQP 147

Query: 214 ---------------REKKLLSSVSTSLALATGLGIAEAVMLSLGSGTLMDIMGIPVDSS 273
                          +EK+ + + ST++ L   LG+ +A+ L   S  L+ +MG+  +S 
Sbjct: 148 PAPDTKSNSGNKSNKKEKRTIRTASTAMILGLILGLVQAIFLIFSSKLLLGVMGVKPNSP 207

Query: 274 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 333
           M +PA ++LS+RA GAP ++++LA QG FRGFKDTKTPL+AT   +++N VLDP+ IF  
Sbjct: 208 MLSPAHKYLSIRALGAPALLLSLAMQGIFRGFKDTKTPLFATVVADVINIVLDPIFIFVL 267

Query: 334 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVL 393
             GI GAAIA VIS+Y +  +L   L  +++    +    +  R+L++G LL+ART+AV 
Sbjct: 268 RLGIIGAAIAHVISQYFMTLILFVFLAKKVNLIPPNFGDLQFGRFLKNGLLLLARTIAVT 327

Query: 394 VTLTLATSMAAREGPVPMAGYQICVQIWMAISLLTDALALAGQALLAGSFTLQDYEHSRQ 453
              TLA +MAAR G  PMA +QIC+Q+W+  SLL D LA+AGQA+LA SF  +DY     
Sbjct: 328 FCQTLAAAMAARLGTTPMAAFQICLQVWLTSSLLNDGLAVAGQAILACSFAEKDYNKVTA 387

Query: 454 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGLLFVAGSQPVNALAFV 513
           V  R LQ+G + G+ L++ + LG    +G+FS D  V+     G+ F+A +QP+N+LAFV
Sbjct: 388 VASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDPAVIHLMAIGIPFIAATQPINSLAFV 447

Query: 514 VDGLYYGVSDFGYAAYSMVLVGLVSSIYLLVVTPTFGLPGVWSGLFLFMMLRLVAGVWRL 546
           +DG+ +G SDF Y AYSMV V  +S   ++ +  T G  G+W  L ++M LR + G+ R+
Sbjct: 448 LDGVNFGASDFAYTAYSMVGVAAISIAAVIYMAKTNGFIGIWIALTIYMALRAITGIARM 507