CSPI04G14850.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI04G14850.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	subtilisin inhibitor-like
Location	Chr4: 12429374 .. 12429731 (+)
Sequence length	358
RNA-Seq Expression	CSPI04G14850.1
Synteny	CSPI04G14850.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAACATCATCAGTACCTATACCTATTAATATCCATTTTTTAAAACCCCTATAGCAGCAACTTTCTATAGAATATGTATTATTGTACTAAAAAAAATGATTCACACAAATATTACACACAATCTTATAAGGAGCTAATGCAATTATTTCAACCCGAGTCACCAGAGAATAAGGTATGGCCGTCATTAGTGGGTTTGACAGCAGAAGAGGCAGAGAAGAAGATAAAGGAAGAAATGCCAGAGGTTTATGTTACAGTGGTTCCTCCTAATCATGCCATAACTTTTGATCTTCGATTTAACCGTGTTTGGCTATTCGTTGATTCTTCTGGCAAAGTTAGACAAACACCTTCTATTGGTTAA

mRNA sequence

GAAACATCATCAGTACCTATACCTATTAATATCCATTTTTTAAAACCCCTATAGCAGCAACTTTCTATAGAATATGTATTATTGTACTAAAAAAAATGATTCACACAAATATTACACACAATCTTATAAGGAGCTAATGCAATTATTTCAACCCGAGTCACCAGAGAATAAGGTATGGCCGTCATTAGTGGGTTTGACAGCAGAAGAGGCAGAGAAGAAGATAAAGGAAGAAATGCCAGAGGTTTATGTTACAGTGGTTCCTCCTAATCATGCCATAACTTTTGATCTTCGATTTAACCGTGTTTGGCTATTCGTTGATTCTTCTGGCAAAGTTAGACAAACACCTTCTATTGGTTAA

Coding sequence (CDS)

ATGTATTATTGTACTAAAAAAAATGATTCACACAAATATTACACACAATCTTATAAGGAGCTAATGCAATTATTTCAACCCGAGTCACCAGAGAATAAGGTATGGCCGTCATTAGTGGGTTTGACAGCAGAAGAGGCAGAGAAGAAGATAAAGGAAGAAATGCCAGAGGTTTATGTTACAGTGGTTCCTCCTAATCATGCCATAACTTTTGATCTTCGATTTAACCGTGTTTGGCTATTCGTTGATTCTTCTGGCAAAGTTAGACAAACACCTTCTATTGGTTAA

Protein sequence

MYYCTKKNDSHKYYTQSYKELMQLFQPESPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFNRVWLFVDSSGKVRQTPSIG*

Homology

[Close]

BLAST of CSPI04G14850.1 vs. ExPASy Swiss-Prot
Match: P16064 (Subtilisin inhibitor 1 OS=Phaseolus angularis OX=3914 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 4.0e-12
Identity = 38/87 (43.68%), Postives = 50/87 (57.47%), Query Frame = 0


Query: 8  NDSHKYYTQSYKELMQLFQPESPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHA 67
          N S +      +   Q  +  +P    WP LVG+TAE+AE KIKEEM +V + V P +  
Sbjct: 6  NPSQEQNVPLPRNYKQALETNTPTKTSWPELVGVTAEQAETKIKEEMVDVQIQVSPHDSF 65

Query: 68 ITFDLRFNRVWLFVDSSGKVRQTPSIG 95
          +T D    RV L+VD S KV +TPSIG
Sbjct: 66 VTADYNPKRVRLYVDESNKVTRTPSIG 92

[Close]

BLAST of CSPI04G14850.1 vs. ExPASy Swiss-Prot
Match: P81712 (Subtilisin inhibitor CLSI-I OS=Canavalia lineata OX=28957 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.5e-11
Identity = 36/60 (60.00%), Postives = 42/60 (70.00%), Query Frame = 0


Query: 35 WPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFNRVWLFVDSSGKVRQTPSIG 94
          WP LVG+TAEEAE KIKEEM  V + VVPP   +T D +  RV L+VD S KV +TP IG
Sbjct: 7  WPELVGVTAEEAE-KIKEEMSGVEIQVVPPGSFVTADYKPQRVRLYVDESNKVTRTPGIG 65

[Close]

BLAST of CSPI04G14850.1 vs. ExPASy Swiss-Prot
Match: P08820 (Subtilisin inhibitor (Fragment) OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 1.3e-10
Identity = 33/60 (55.00%), Postives = 42/60 (70.00%), Query Frame = 0


Query: 35 WPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFNRVWLFVDSSGKVRQTPSIG 94
          WP LVG++AEEA +KIKEEMPE  + VVP +  +T D +F RV L+VD S KV +   IG
Sbjct: 4  WPELVGVSAEEA-RKIKEEMPEAEIQVVPQDSFVTADYKFQRVRLYVDESNKVVRAAPIG 62

[Close]

BLAST of CSPI04G14850.1 vs. ExPASy Swiss-Prot
Match: P83472 (Chymotrypsin inhibitor OS=Lumbricus terrestris OX=6398 PE=1 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 1.6e-08
Identity = 26/60 (43.33%), Postives = 39/60 (65.00%), Query Frame = 0


Query: 35 WPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFNRVWLFVDSSGKVRQTPSIG 94
          WP LVG T EEA+ +I E+ P+  + V P +  +T+D R +RV +FV+  G V +TP+ G
Sbjct: 27 WPELVGETLEEAKAQILEDRPDAVIKVQPEHSPVTYDYRPSRVIIFVNKDGNVAETPAAG 86

[Close]

BLAST of CSPI04G14850.1 vs. ExPASy Swiss-Prot
Match: P82977 (Subtilisin-chymotrypsin inhibitor WSCI OS=Triticum aestivum OX=4565 PE=1 SV=2)

HSP 1 Score: 58.9 bits (141), Expect = 3.5e-08
Identity = 26/60 (43.33%), Postives = 37/60 (61.67%), Query Frame = 0


Query: 35 WPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFNRVWLFVDSSGKVRQTPSIG 94
          WP LVG + EEA+K I ++  E  + V+P    +T + R +RV LFVDS  K+ Q P +G
Sbjct: 25 WPELVGKSVEEAKKVILQDKSEAQIVVLPVGTIVTMEYRIDRVRLFVDSLDKIAQVPRVG 84

[Close]

BLAST of CSPI04G14850.1 vs. ExPASy TrEMBL
Match: A0A0A0KX92 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G296240 PE=3 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 2.4e-36
Identity = 77/79 (97.47%), Postives = 78/79 (98.73%), Query Frame = 0


Query: 16  QSYKELMQLFQPESPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFN 75
           +SYKELMQLFQPE PENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFN
Sbjct: 26  KSYKELMQLFQPEPPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFN 85

Query: 76  RVWLFVDSSGKVRQTPSIG 95
           RVWLFVDSSGKVRQTPSIG
Sbjct: 86  RVWLFVDSSGKVRQTPSIG 104

[Close]

BLAST of CSPI04G14850.1 vs. ExPASy TrEMBL
Match: A0A5D3DJ17 (Subtilisin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold500G00580 PE=3 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 5.3e-23
Identity = 59/77 (76.62%), Postives = 66/77 (85.71%), Query Frame = 0


Query: 22 MQLFQPESPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPP-----NHAITFDLRFNR 81
          MQLFQPE+P+NKVW SLV LTAEEAEKKIKEEMPEVYV+VVPP      +++T DLRFNR
Sbjct: 1  MQLFQPETPKNKVWLSLVSLTAEEAEKKIKEEMPEVYVSVVPPGYGSSGYSMTSDLRFNR 60

Query: 82 VWLFVDSSGKVRQTPSI 94
          VWLFVDSSGKV QT S+
Sbjct: 61 VWLFVDSSGKVIQTLSL 77

[Close]

BLAST of CSPI04G14850.1 vs. ExPASy TrEMBL
Match: A0A067EAY7 (Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g034123mg PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 1.6e-16
Identity = 46/72 (63.89%), Postives = 52/72 (72.22%), Query Frame = 0


Query: 23  QLFQPESPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFNRVWLFVD 82
           QL  P +   K WP LVGLTAEEAEKKIKEE P   V VVPPN+ +T D R NRV L+VD
Sbjct: 32  QLLGPNAASIKTWPGLVGLTAEEAEKKIKEERPGAQVQVVPPNNFVTMDFRHNRVRLYVD 91

Query: 83  SSGKVRQTPSIG 95
           SSGKV + PS+G
Sbjct: 92  SSGKVERPPSVG 103

[Close]

BLAST of CSPI04G14850.1 vs. ExPASy TrEMBL
Match: A0A2H5P6A3 (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_107860 PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 2.2e-16
Identity = 48/86 (55.81%), Postives = 55/86 (63.95%), Query Frame = 0


Query: 9   DSHKYYTQSYKELMQLFQPESPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAI 68
           + H+ Y        QL  P +   K WP LVGLTAEEAEKKIKEE P   V VVPPN  +
Sbjct: 83  NQHRLYQLDTGTYGQLLGPNAAAIKTWPGLVGLTAEEAEKKIKEERPGAQVQVVPPNSFV 142

Query: 69  TFDLRFNRVWLFVDSSGKVRQTPSIG 95
           T D R NRV L+VDSSGKV + PS+G
Sbjct: 143 TMDFRHNRVRLYVDSSGKVERPPSVG 168

[Close]

BLAST of CSPI04G14850.1 vs. ExPASy TrEMBL
Match: A0A2H5P6F7 (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_107850 PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 3.7e-16
Identity = 46/72 (63.89%), Postives = 51/72 (70.83%), Query Frame = 0


Query: 23  QLFQPESPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFNRVWLFVD 82
           QL  P +   K WP LVGLTAEEAEKKIKEE P   V VVPPN  +T D R NRV L+VD
Sbjct: 34  QLLGPNAAAIKTWPGLVGLTAEEAEKKIKEERPGAQVQVVPPNSFVTMDFRHNRVRLYVD 93

Query: 83  SSGKVRQTPSIG 95
           SSGKV + PS+G
Sbjct: 94  SSGKVERPPSVG 105

[Close]

BLAST of CSPI04G14850.1 vs. NCBI nr
Match: XP_031740562.1 (subtilisin inhibitor CLSI-I isoform X1 [Cucumis sativus])

HSP 1 Score: 156.4 bits (394), Expect = 1.2e-34
Identity = 75/78 (96.15%), Postives = 76/78 (97.44%), Query Frame = 0


Query: 17  SYKELMQLFQPESPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFNR 76
           S+ ELMQLFQPE PENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFNR
Sbjct: 40  SFLELMQLFQPEPPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFNR 99

Query: 77  VWLFVDSSGKVRQTPSIG 95
           VWLFVDSSGKVRQTPSIG
Sbjct: 100 VWLFVDSSGKVRQTPSIG 117

[Close]

BLAST of CSPI04G14850.1 vs. NCBI nr
Match: XP_031740563.1 (subtilisin inhibitor CLSI-I isoform X2 [Cucumis sativus] >KGN54567.2 hypothetical protein Csa_018033 [Cucumis sativus])

HSP 1 Score: 136.7 bits (343), Expect = 1.0e-28
Identity = 67/86 (77.91%), Postives = 73/86 (84.88%), Query Frame = 0


Query: 9  DSHKYYTQSYKELMQLFQPESPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAI 68
          D+ K     Y+++ +      P+NKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAI
Sbjct: 4  DNQKSKAPQYQKVGESTTSIIPKNKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAI 63

Query: 69 TFDLRFNRVWLFVDSSGKVRQTPSIG 95
          TFDLRFNRVWLFVDSSGKVRQTPSIG
Sbjct: 64 TFDLRFNRVWLFVDSSGKVRQTPSIG 89

[Close]

BLAST of CSPI04G14850.1 vs. NCBI nr
Match: XP_038880794.1 (subtilisin inhibitor CLSI-I-like [Benincasa hispida])

HSP 1 Score: 132.5 bits (332), Expect = 1.9e-27
Identity = 64/79 (81.01%), Postives = 71/79 (89.87%), Query Frame = 0


Query: 16  QSYKELMQLFQPESPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFN 75
           +SY+E MQLFQP++  NK WPSLVGLTAEEAEKKIKEEM EVYV+VVPP++AITFDLR N
Sbjct: 26  KSYEEFMQLFQPKTSVNKAWPSLVGLTAEEAEKKIKEEMAEVYVSVVPPSYAITFDLRSN 85

Query: 76  RVWLFVDSSGKVRQTPSIG 95
           RVWLFVDSSGKV QTP IG
Sbjct: 86  RVWLFVDSSGKVVQTPVIG 104

[Close]

BLAST of CSPI04G14850.1 vs. NCBI nr
Match: KAA0032671.1 (subtilisin inhibitor 1 [Cucumis melo var. makuwa] >TYK23552.1 subtilisin inhibitor 1 [Cucumis melo var. makuwa])

HSP 1 Score: 116.7 bits (291), Expect = 1.1e-22
Identity = 59/77 (76.62%), Postives = 66/77 (85.71%), Query Frame = 0


Query: 22 MQLFQPESPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPP-----NHAITFDLRFNR 81
          MQLFQPE+P+NKVW SLV LTAEEAEKKIKEEMPEVYV+VVPP      +++T DLRFNR
Sbjct: 1  MQLFQPETPKNKVWLSLVSLTAEEAEKKIKEEMPEVYVSVVPPGYGSSGYSMTSDLRFNR 60

Query: 82 VWLFVDSSGKVRQTPSI 94
          VWLFVDSSGKV QT S+
Sbjct: 61 VWLFVDSSGKVIQTLSL 77

[Close]

BLAST of CSPI04G14850.1 vs. NCBI nr
Match: KDO52364.1 (hypothetical protein CISIN_1g034123mg [Citrus sinensis])

HSP 1 Score: 95.1 bits (235), Expect = 3.4e-16
Identity = 46/72 (63.89%), Postives = 52/72 (72.22%), Query Frame = 0


Query: 23  QLFQPESPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFNRVWLFVD 82
           QL  P +   K WP LVGLTAEEAEKKIKEE P   V VVPPN+ +T D R NRV L+VD
Sbjct: 32  QLLGPNAASIKTWPGLVGLTAEEAEKKIKEERPGAQVQVVPPNNFVTMDFRHNRVRLYVD 91

Query: 83  SSGKVRQTPSIG 95
           SSGKV + PS+G
Sbjct: 92  SSGKVERPPSVG 103

[Close]

BLAST of CSPI04G14850.1 vs. TAIR 10
Match: AT2G38870.1 (Serine protease inhibitor, potato inhibitor I-type family protein )

HSP 1 Score: 46.6 bits (109), Expect = 1.3e-05
Identity = 24/67 (35.82%), Postives = 32/67 (47.76%), Query Frame = 0


Query: 28 ESPENKVWPSLVGLTAEEAEKKIKEEMPEVYVTVVPPNHAITFDLRFNRVWLFVDSSGKV 87
          E P    WP L G   + A   I+ E P V   V+     +T D R +RV +FVD +  V
Sbjct: 4  ECPRKNSWPELTGTNGDYAAVVIERENPTVNAAVILDGSPVTADFRCDRVRVFVDGNRIV 63

Query: 88 RQTPSIG 95
           +TP  G
Sbjct: 64 VKTPKSG 70

The following BLAST results are available for this feature:

BLAST of CSPI04G14850.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P16064	4.0e-12	43.68	Subtilisin inhibitor 1 OS=Phaseolus angularis OX=3914 PE=1 SV=1	[more]
P81712	1.5e-11	60.00	Subtilisin inhibitor CLSI-I OS=Canavalia lineata OX=28957 PE=1 SV=1	[more]
P08820	1.3e-10	55.00	Subtilisin inhibitor (Fragment) OS=Vicia faba OX=3906 PE=1 SV=1	[more]
P83472	1.6e-08	43.33	Chymotrypsin inhibitor OS=Lumbricus terrestris OX=6398 PE=1 SV=2	[more]
P82977	3.5e-08	43.33	Subtilisin-chymotrypsin inhibitor WSCI OS=Triticum aestivum OX=4565 PE=1 SV=2	[more]

BLAST of CSPI04G14850.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KX92	2.4e-36	97.47	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G296240 PE=3 SV=1	[more]
A0A5D3DJ17	5.3e-23	76.62	Subtilisin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5...	[more]
A0A067EAY7	1.6e-16	63.89	Uncharacterized protein OS=Citrus sinensis OX=2711 GN=CISIN_1g034123mg PE=3 SV=1	[more]
A0A2H5P6A3	2.2e-16	55.81	Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_107860 PE=3 SV=1	[more]
A0A2H5P6F7	3.7e-16	63.89	Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_107850 PE=3 SV=1	[more]

BLAST of CSPI04G14850.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031740562.1	1.2e-34	96.15	subtilisin inhibitor CLSI-I isoform X1 [Cucumis sativus]	[more]
XP_031740563.1	1.0e-28	77.91	subtilisin inhibitor CLSI-I isoform X2 [Cucumis sativus] >KGN54567.2 hypothetica...	[more]
XP_038880794.1	1.9e-27	81.01	subtilisin inhibitor CLSI-I-like [Benincasa hispida]	[more]
KAA0032671.1	1.1e-22	76.62	subtilisin inhibitor 1 [Cucumis melo var. makuwa] >TYK23552.1 subtilisin inhibit...	[more]
KDO52364.1	3.4e-16	63.89	hypothetical protein CISIN_1g034123mg [Citrus sinensis]	[more]

BLAST of CSPI04G14850.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38870.1	1.3e-05	35.82	Serine protease inhibitor, potato inhibitor I-type family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PRINTS	PR00292	POTATOINHBTR	coord: 59..70 score: 19.93 coord: 34..47 score: 45.34 coord: 71..81 score: 57.71
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PFAM	PF00280	potato_inhibit	coord: 35..94 e-value: 2.9E-20 score: 72.7
IPR000864	Proteinase inhibitor I13, potato inhibitor I	PANTHER	PTHR33091	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 17..94
None	No IPR available	GENE3D	3.30.10.10	Trypsin Inhibitor V, subunit A	coord: 27..94 e-value: 3.1E-22 score: 80.7
None	No IPR available	PANTHER	PTHR33091:SF29	PROTEIN, PUTATIVE, EXPRESSED-RELATED	coord: 17..94
IPR036354	Proteinase inhibitor I13, potato inhibitor I superfamily	SUPERFAMILY	54654	CI-2 family of serine protease inhibitors	coord: 32..94

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI04G14850	CSPI04G14850	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G14850.1.utr5p1	CSPI04G14850.1.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI04G14850.1.cds1	CSPI04G14850.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI04G14850.1	CSPI04G14850.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009611	response to wounding
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity