CSPI03G38820.2 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI03G38820.2
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Dihydroorotase
Location	Chr3: 33434310 .. 33437608 (-)
Sequence length	1715
RNA-Seq Expression	CSPI03G38820.2
Synteny	CSPI03G38820.2

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTAATTATAACAACATCCATAAAAAAACTATTGAAAATTGTATGGATTTCATTTCCAATTAACACCATATTTCAAACCTTGTTAGTAAGGGCTTCAATTTTGTCTTATTTGTTTAATTATCCATCCCATCTTTCCTCAAGCTCCAAGCACCTCACGGCGAATCCACCATCTCCGGCTATCCTCAGGCGACCCAACTCAATCACAAAGAGCCGGACCGTCCTGGCTCTTCCTACTCCGGCGGATTCAGTTCCTCGCGGCAGCAGATCTACAAGTGCAAGTGACCCACGAACGGCTCCTGCGACGTTTCCCACTTCTTCTTCTTCTTCTCCGGGCAACAAGCTGCACCGGCGCTAGGTAAGCTTCGAAAACAAATCAACAACGGCGGCATTTGGTTGTGCATCTGTTATCTCTGATTTTATATTTTCATTTGAACAGAAGAAAAATCGTTTTAAAGAAGCCTCAAGGTTGTCTCTGGATTTTACTATGACAAAGACTTTGGTCCTTCCGTTCCAAGTACATATGCTTTAGTTATCAATAAAATCCAATCTATTTTATATTCAATTTGTATTTGGTTCATTTTGTTGTCTGTCATCAACTCTTCTTCGAGCAACGTTCATGTGGCGTATTATAGTTCAAGCATTGTAGGATGTACTTTGAGCGTCATCTTCGGTGTGATATTTTCGTAGTTTCCTTAACCAATGACAATTTACTAAACTTAAATTCAGATCTCTGCTTTCATTTCACTGATTGCTACATTGTTGATGGTAGCCACTGAAATCACCTGCACAAAAGTTTGAAGGGGTTAAGTTCATAAGGCGCCAAGGGCCAAAAATGCAGCTCACACTCACCCAGCCTGATGATTGGCATCTTCATCTTCGCGATGGTGATCTCTTAAAAGCGGTCCTCCCTCACAGGTAAGCTTATCTTTTATTCTTTGTACATGGTGGTAGTGGTTTTGAAATGATTAAAATCACTTTTGTCAAGATTAAGTTACTCTAATACATGCTTTCAATCATTCATAGTCAATTTTAGTTGTATAAAAATAATGTTTAAAAGTGTAAAATCCAGCATTGGGTTGCTTTTCATTGATTGAGATATGTTTATGGGTGATTTTGAAGGTGTCAACAGTTTTAGAATCACTCCTAAACATATCATAATTGACATGTCAAGCAACCACATGATGCATTCTATTTTAACATGTATATCATGTTTTCAATTGTTCATTCTTGTCCTAAACTATCAATATGCAGTGCAAGTACCTTTGGAAGGGCTATTGTGATGCCAAATCTGAAGCCACCTGTCACTACCACAGCTGCTGCTGTGGCGTATCGGGATTCTATCTTGAAAGCGTTACCGTCCAATAGCAACTTTGTTCCTCTTATGACCCTTTATTTGACGGATACAATGAGCCCAAACGAGATCAAGCTTGCAAGTAAGTCTTTGCTTTCAACAAGAAATTTTGGAAGAGATCTGTTATTTGATGATTTTTGAGGTTGTATTCTATTCTTTTTTCTAACATGATGATGGATTGACAGGAAAAAGTGGGGCCGTTTATGCTGTAAAATTGTATCCTGCCGGTGCTACGACCAATTCCCAAGATGGTGTTACAGATCTTTTTGGGAAGTGTCTACCTGTTCTTGAGGAGATGGTTGAACAGGATATGCCTCTGTTGGTAGTTTGCTGATAGGAACACTTTGTTTATTCGTCCAATATATAGAGAAGCAGTGCATTCTTTCTTCTTGTTAATTTAAGAAGAGATATCTCTCTGTAGGTTCATGGTGAGGTAACAAATTCTGATGTTGACATGTTTGATCGTGAGAAGGTCTTCATTGAAACTGTGTTGAAACCCCTGATTGAGAGGCTTCCAAAGCTGAAGGTGGTGATGGAGCATATTACTACTGCAGATGCTGCTAAGTTTGTTTTGTCTTGCGAACATGGTATTTATACTTCATTATTTTCATTGAAGTGTTTTTGTTAGTTATCCCTCTTTCATTTTGTCGACCATTGGTATCACTTTTTTCTTGTAAATTATGCTTACCAATATATGACTTTTAAAATCCTTTACAGCGCTTCTAGCAGCAACTGTCACTCCACAACATCTTCTTCTCAACAGGAATTCTCTATTTCAAGGAGGACTGCAACCACATAATTACTGTCTTCCGGTACTCAAAAGAGAGACTCACAGTATGTTCCAACTATATTTTGTGCTTATTCTTTATATTTTCCTCACACATGTTCTTCTTGTATGACTGTATCAGTAGCCTTCATGAAAAACAATTTCTCCTTACAAGTATAAGATATTCTTATCCAAGTTAGAAGGTTTTTTGGAGGTTAGGATGGATTAACCTCAACATAAGGTGGCTTTTGATATTCGTCATGTATCTTAGAATCATTGAAGTTGGCCTAGTGGTCAATATTAATTATAAAAGGCTTTGGGTGAATGAGTTCAAGCCATGGTTGTCACCTTCTTAAGATTTAATACCTCATGAGTTTTTTTGTTTGGACTTTTCTTCATGAAACTTGAGAATCCCCCAAAAGAAAGATAATTATTTCTTCATTTGTGCTCACGCCTTTTCTCTCATCTAGGGCAGGCAATTGTGTCAGCTGTAACAAGCGGAGGCAAAAAGTTCTTCCTCGGAACTGACAGCGCTCCACATGAGAGAAAAAGGAAAGAGAGTTCATGTGGTTGTGCCGGTATATACAGTGCCCCTATTGCCTTGTCATTATATGCAAAGGTGTTTGAAGAGGTATACCATCTTGTTTCTTATTATGCTAATAATTTGAATCACTTCATCTTTATCAAATAATCATCATTTTCTCCTTTTTGGTTTCTTTTTCTCTGCCAAGGCGGGTGCTCTTGATAAACTAGAAGCATTTACAAGTTTCAATGGACCCGACTTCTATGGACTCCCCAGAAACACCTCAAAAATTACACTGCAAAAGGCTCCATGGCAGGTCCCAAAGTCTTTTTCATTTTCTTTTGGGGATATTGTTCCAATGTTTGCAGGTGAGACACTTGAGTGGCAGCCATGCTTCAACTGAGACTAACAAGTTCCAATGTTGCTTGCTTACACAATAACACCACATTCTAAGCATGTCCTCAATTCTCTTGTTTAACTACACTGCTGTTGATTTACCTGAATTAGAGCTTTTTACTGTTGGGACATCTATGATTGTTCCCTTCCCTCCCATTTTTGAAATGAGATCATGATGAACTTTTGATTTGTATGAGATTTTGAAGAAGTTGATCTTGGATATTGTTGGTTTGGTAATAAAAATTCCCAAAATTTGTTTTTGG

mRNA sequence

ATTAATTATAACAACATCCATAAAAAAACTATTGAAAATTGTATGGATTTCATTTCCAATTAACACCATATTTCAAACCTTGTTAGTAAGGGCTTCAATTTTGTCTTATTTGTTTAATTATCCATCCCATCTTTCCTCAAGCTCCAAGCACCTCACGGCGAATCCACCATCTCCGGCTATCCTCAGGCGACCCAACTCAATCACAAAGAGCCGGACCGTCCTGGCTCTTCCTACTCCGGCGGATTCAGTTCCTCGCGGCAGCAGATCTACAAGTGCAAGTGACCCACGAACGGCTCCTGCGACGTTTCCCACTTCTTCTTCTTCTTCTCCGGGCAACAAGCTGCACCGGCGCTAGCCACTGAAATCACCTGCACAAAAGTTTGAAGGGGTTAAGTTCATAAGGCGCCAAGGGCCAAAAATGCAGCTCACACTCACCCAGCCTGATGATTGGCATCTTCATCTTCGCGATGGTGATCTCTTAAAAGCGGTCCTCCCTCACAGTGCAAGTACCTTTGGAAGGGCTATTGTGATGCCAAATCTGAAGCCACCTGTCACTACCACAGCTGCTGCTGTGGCGTATCGGGATTCTATCTTGAAAGCGTTACCGTCCAATAGCAACTTTGTTCCTCTTATGACCCTTTATTTGACGGATACAATGAGCCCAAACGAGATCAAGCTTGCAAGAAAAAGTGGGGCCGTTTATGCTGTAAAATTGTATCCTGCCGGTGCTACGACCAATTCCCAAGATGGTGTTACAGATCTTTTTGGGAAGTGTCTACCTGTTCTTGAGGAGATGGTTGAACAGGATATGCCTCTGTTGGTTCATGGTGAGGTAACAAATTCTGATGTTGACATGTTTGATCGTGAGAAGGTCTTCATTGAAACTGTGTTGAAACCCCTGATTGAGAGGCTTCCAAAGCTGAAGGTGGTGATGGAGCATATTACTACTGCAGATGCTGCTAAGTTTGTTTTGTCTTGCGAACATGCGCTTCTAGCAGCAACTGTCACTCCACAACATCTTCTTCTCAACAGGAATTCTCTATTTCAAGGAGGACTGCAACCACATAATTACTGTCTTCCGGTACTCAAAAGAGAGACTCACAGGCAGGCAATTGTGTCAGCTGTAACAAGCGGAGGCAAAAAGTTCTTCCTCGGAACTGACAGCGCTCCACATGAGAGAAAAAGGAAAGAGAGTTCATGTGGTTGTGCCGGTATATACAGTGCCCCTATTGCCTTGTCATTATATGCAAAGGTGTTTGAAGAGGCGGGTGCTCTTGATAAACTAGAAGCATTTACAAGTTTCAATGGACCCGACTTCTATGGACTCCCCAGAAACACCTCAAAAATTACACTGCAAAAGGCTCCATGGCAGGTCCCAAAGTCTTTTTCATTTTCTTTTGGGGATATTGTTCCAATGTTTGCAGGTGAGACACTTGAGTGGCAGCCATGCTTCAACTGAGACTAACAAGTTCCAATGTTGCTTGCTTACACAATAACACCACATTCTAAGCATGTCCTCAATTCTCTTGTTTAACTACACTGCTGTTGATTTACCTGAATTAGAGCTTTTTACTGTTGGGACATCTATGATTGTTCCCTTCCCTCCCATTTTTGAAATGAGATCATGATGAACTTTTGATTTGTATGAGATTTTGAAGAAGTTGATCTTGGATATTGTTGGTTTGGTAATAAAAATTCCCAAAATTTGTTTTTGG

Coding sequence (CDS)

ATGCAGCTCACACTCACCCAGCCTGATGATTGGCATCTTCATCTTCGCGATGGTGATCTCTTAAAAGCGGTCCTCCCTCACAGTGCAAGTACCTTTGGAAGGGCTATTGTGATGCCAAATCTGAAGCCACCTGTCACTACCACAGCTGCTGCTGTGGCGTATCGGGATTCTATCTTGAAAGCGTTACCGTCCAATAGCAACTTTGTTCCTCTTATGACCCTTTATTTGACGGATACAATGAGCCCAAACGAGATCAAGCTTGCAAGAAAAAGTGGGGCCGTTTATGCTGTAAAATTGTATCCTGCCGGTGCTACGACCAATTCCCAAGATGGTGTTACAGATCTTTTTGGGAAGTGTCTACCTGTTCTTGAGGAGATGGTTGAACAGGATATGCCTCTGTTGGTTCATGGTGAGGTAACAAATTCTGATGTTGACATGTTTGATCGTGAGAAGGTCTTCATTGAAACTGTGTTGAAACCCCTGATTGAGAGGCTTCCAAAGCTGAAGGTGGTGATGGAGCATATTACTACTGCAGATGCTGCTAAGTTTGTTTTGTCTTGCGAACATGCGCTTCTAGCAGCAACTGTCACTCCACAACATCTTCTTCTCAACAGGAATTCTCTATTTCAAGGAGGACTGCAACCACATAATTACTGTCTTCCGGTACTCAAAAGAGAGACTCACAGGCAGGCAATTGTGTCAGCTGTAACAAGCGGAGGCAAAAAGTTCTTCCTCGGAACTGACAGCGCTCCACATGAGAGAAAAAGGAAAGAGAGTTCATGTGGTTGTGCCGGTATATACAGTGCCCCTATTGCCTTGTCATTATATGCAAAGGTGTTTGAAGAGGCGGGTGCTCTTGATAAACTAGAAGCATTTACAAGTTTCAATGGACCCGACTTCTATGGACTCCCCAGAAACACCTCAAAAATTACACTGCAAAAGGCTCCATGGCAGGTCCCAAAGTCTTTTTCATTTTCTTTTGGGGATATTGTTCCAATGTTTGCAGGTGAGACACTTGAGTGGCAGCCATGCTTCAACTGA

Protein sequence

MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILKALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLPVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADAAKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGGKKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDFYGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN*

Homology

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BLAST of CSPI03G38820.2 vs. ExPASy Swiss-Prot
Match: O04904 (Dihydroorotase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PYR4 PE=1 SV=1)

HSP 1 Score: 588.6 bits (1516), Expect = 4.7e-167
Identity = 283/343 (82.51%), Postives = 313/343 (91.25%), Query Frame = 0


Query: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 60
           M+LT+TQPDDWHLHLRDGDLL AV+PHSAS F RAIVMPNLKPPVT+TAAA+ YR  I+K
Sbjct: 33  MELTITQPDDWHLHLRDGDLLHAVVPHSASNFKRAIVMPNLKPPVTSTAAAIIYRKFIMK 92

Query: 61  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120
           ALPS S+F PLMTLYLTD   P EI+LAR+SG VYAVKLYPAGATTNSQDGVTDLFGKCL
Sbjct: 93  ALPSESSFDPLMTLYLTDKTLPEEIRLARESGVVYAVKLYPAGATTNSQDGVTDLFGKCL 152

Query: 121 PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA 180
           PVLEEMV+Q+MPLLVHGEVT+  +D+FDREK+FIETVL+PLI+RLP+LKVVMEHITT DA
Sbjct: 153 PVLEEMVKQNMPLLVHGEVTDPSIDVFDREKIFIETVLQPLIQRLPQLKVVMEHITTMDA 212

Query: 181 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGG 240
             FV SC+   + ATVTPQHLLLNRN+LFQGGLQPHNYCLPVLKRE HR+AIV AVTSG 
Sbjct: 213 VNFVESCKEGSVGATVTPQHLLLNRNALFQGGLQPHNYCLPVLKREIHREAIVKAVTSGS 272

Query: 241 KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 300
           KKFFLGTDSAPHER RKESSCGCAGIYSAPIALSLYAKVF+EAGALDKLEAFTSFNGPDF
Sbjct: 273 KKFFLGTDSAPHERSRKESSCGCAGIYSAPIALSLYAKVFDEAGALDKLEAFTSFNGPDF 332

Query: 301 YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQP 344
           YGLPRN+SKITL+K+PW+VP  F+F FG+IVPMFAGETL+WQP
Sbjct: 333 YGLPRNSSKITLKKSPWKVPDVFNFPFGEIVPMFAGETLQWQP 375

[Close]

BLAST of CSPI03G38820.2 vs. ExPASy Swiss-Prot
Match: Q0JJD1 (Dihydroorotase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=PYRC PE=2 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 4.6e-162
Identity = 271/342 (79.24%), Postives = 307/342 (89.77%), Query Frame = 0


Query: 2   QLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILKA 61
           +LT+T+PDDWHLHLR+GD+L AVLPHSA  FGRAIVMPNLKPPVTTTA A+ YR+ IL+A
Sbjct: 59  ELTITRPDDWHLHLREGDVLAAVLPHSAMHFGRAIVMPNLKPPVTTTARALEYREEILRA 118

Query: 62  LPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLP 121
           LP  SNFVPLMTLYLTD  SP EIKLA+KSG V+AVKLYP+GATTNSQDGVTD+FGKCLP
Sbjct: 119 LPPGSNFVPLMTLYLTDNTSPEEIKLAKKSGVVFAVKLYPSGATTNSQDGVTDIFGKCLP 178

Query: 122 VLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADAA 181
           VLEEM  Q+MPLLVHGEVT+  VD FDREKVFIE +L PL++RLP+LK+VMEHITT DA 
Sbjct: 179 VLEEMARQEMPLLVHGEVTDQHVDTFDREKVFIEKILAPLVQRLPQLKIVMEHITTMDAV 238

Query: 182 KFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGGK 241
            FV SC+   +AATVTPQHLLLNRN+LFQGGLQPHNYCLPVLKRETHRQAIVSAVTSG K
Sbjct: 239 NFVESCKEGHVAATVTPQHLLLNRNALFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGSK 298

Query: 242 KFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDFY 301
           ++FLGTDSAPH+++RKE SCGCAGIYSAP+ALSLYAKVFE+AGALDKLEAFTSFNGPDFY
Sbjct: 299 QYFLGTDSAPHDKRRKECSCGCAGIYSAPVALSLYAKVFEQAGALDKLEAFTSFNGPDFY 358

Query: 302 GLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQP 344
           GLPRNTSKI L+K+ W+VP ++S+S G+IVPMF G TLEW P
Sbjct: 359 GLPRNTSKIVLRKSAWKVPDTYSYSSGEIVPMFTGNTLEWLP 400

[Close]

BLAST of CSPI03G38820.2 vs. ExPASy Swiss-Prot
Match: B1XI96 (Dihydroorotase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) OX=32049 GN=pyrC PE=3 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 1.8e-126
Identity = 224/341 (65.69%), Postives = 262/341 (76.83%), Query Frame = 0


Query: 2   QLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILKA 61
           ++TLT+PDDWHLHLRDG+ LKAVLP +   F RAIVMPNLKPPV T A A AY D IL A
Sbjct: 3   KITLTRPDDWHLHLRDGEALKAVLPDTVRQFARAIVMPNLKPPVRTVAEAAAYHDRILAA 62

Query: 62  LPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLP 121
           +P    F PLMTLYLTD  SP EI+ A++SG V AVK YPAGATTNS  GVTD+  KC  
Sbjct: 63  IPPGRTFEPLMTLYLTDNTSPAEIQAAKESGFVKAVKYYPAGATTNSDLGVTDI-RKCDA 122

Query: 122 VLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADAA 181
           V E M   D+PLL+HGEVT+  VD+FDREKVFIET L+PL ER P+L++V+EHITT DA 
Sbjct: 123 VFEAMQTVDLPLLLHGEVTDHRVDVFDREKVFIETYLRPLKERFPQLRIVLEHITTKDAV 182

Query: 182 KFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGGK 241
           +FVL+ +   +AAT+TPQHLL NRNS+FQGG++PH YCLP+LKRETHR+A++ A TSG  
Sbjct: 183 EFVLASDEK-VAATITPQHLLFNRNSIFQGGIRPHFYCLPILKRETHREALLEAATSGNP 242

Query: 242 KFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDFY 301
           KFFLGTDSAPH R RKES CGCAG YSA  AL LYA VFE A ALDKLE F SF GPDFY
Sbjct: 243 KFFLGTDSAPHGRDRKESDCGCAGCYSALHALELYATVFEAADALDKLEGFASFYGPDFY 302

Query: 302 GLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQ 343
            LPRNT KITL K PWQ+P +  F    +VP++AG+ L W+
Sbjct: 303 QLPRNTEKITLTKTPWQIPDALPFPESSLVPLWAGQELSWK 341

[Close]

BLAST of CSPI03G38820.2 vs. ExPASy Swiss-Prot
Match: C1D672 (Dihydroorotase OS=Laribacter hongkongensis (strain HLHK9) OX=557598 GN=pyrC PE=3 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 4.2e-123
Identity = 223/341 (65.40%), Postives = 259/341 (75.95%), Query Frame = 0


Query: 3   LTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILKAL 62
           LTLT+PDDWHLHLRDG+ L AV  +SA  F RAIVMPNLKPPVTT   A AYR+ IL AL
Sbjct: 4   LTLTRPDDWHLHLRDGEALAAVAAYSAREFARAIVMPNLKPPVTTVVQAAAYRERILAAL 63

Query: 63  -PSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLP 122
            P    F PLMTLYLTD   P EI    +SG V+A+KLYPAGATTNS  GVT L G CLP
Sbjct: 64  PPGGPAFEPLMTLYLTDNTPPAEIDRVAESGFVHALKLYPAGATTNSDAGVTSLSG-CLP 123

Query: 123 VLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADAA 182
            L  M E  +PLLVHGEVT+  +D+FDRE VFI+TVL+PL+  LP L+VVMEHITT  AA
Sbjct: 124 TLTRMAELGVPLLVHGEVTDPQIDVFDREAVFIDTVLQPLLAELPTLRVVMEHITTRQAA 183

Query: 183 KFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGGK 242
           +FVL+     +AATVTPQH+L+NRN+LF GGL+PH+YCLPVLKRE HR AI  AVTSG  
Sbjct: 184 EFVLAAPDN-IAATVTPQHMLMNRNALFTGGLRPHHYCLPVLKREEHRAAIARAVTSGST 243

Query: 243 KFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDFY 302
           +FFLGTDSAPH R  KE++CGCAGI+SA  AL LYA+ FE+AGALD+LEAF SFNGPDFY
Sbjct: 244 RFFLGTDSAPHARHAKEAACGCAGIFSAHAALPLYAEAFEDAGALDRLEAFASFNGPDFY 303

Query: 303 GLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQ 343
            LPRNT  +TL++  W VP S  +    +VP+ AGETL WQ
Sbjct: 304 RLPRNTGTVTLKREEWTVPASLPYGNDTLVPLRAGETLRWQ 342

[Close]

BLAST of CSPI03G38820.2 vs. ExPASy Swiss-Prot
Match: B0CF78 (Dihydroorotase OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=pyrC PE=3 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 7.9e-122
Identity = 217/341 (63.64%), Postives = 256/341 (75.07%), Query Frame = 0


Query: 2   QLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILKA 61
           +LTLT+PDDWHLHLRDGD LKAVLPHSA  F RAIVMPNLKPPV T A A  YR+ IL A
Sbjct: 3   KLTLTRPDDWHLHLRDGDALKAVLPHSARQFARAIVMPNLKPPVRTVADAADYRNRILAA 62

Query: 62  LPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCLP 121
           LP    F PLMTLYLTD  SP EI  A+ S  V AVK YPAGATTNS  GVTD+  KC  
Sbjct: 63  LPVGQTFEPLMTLYLTDNTSPEEISAAKASKFVKAVKYYPAGATTNSDFGVTDI-RKCDR 122

Query: 122 VLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADAA 181
           +   M ++D+PLL+HGEVT++++D+FDREKVFIET L PL+ R PKL+VV+EHITT++A 
Sbjct: 123 IFAAMEQEDIPLLLHGEVTDTNIDVFDREKVFIETHLIPLVSRFPKLRVVLEHITTSEAV 182

Query: 182 KFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGGK 241
            FVL+     + AT+TPQHLL +RN++F+GG++PH YCLP+LKRE HRQA++ A TSG  
Sbjct: 183 TFVLNTNEN-IGATITPQHLLFSRNAIFKGGIRPHYYCLPILKREKHRQALLQAATSGNP 242

Query: 242 KFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDFY 301
           KFFLGTDSAPH R  KE SCGCAG YSA  A+ LYA+ FE A ALDKLEAF SF GPDFY
Sbjct: 243 KFFLGTDSAPHARNSKEQSCGCAGCYSALHAMELYAEAFESANALDKLEAFASFYGPDFY 302

Query: 302 GLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQ 343
            LPRNT  ITL K  WQ+P    F    +VP+ AGE + W+
Sbjct: 303 QLPRNTKTITLAKQTWQIPDEVPFPGTGLVPLRAGEEITWK 341

[Close]

BLAST of CSPI03G38820.2 vs. ExPASy TrEMBL
Match: A0A0A0LG11 (Dihydroorotase OS=Cucumis sativus OX=3659 GN=Csa_3G824970 PE=3 SV=1)

HSP 1 Score: 697.2 bits (1798), Expect = 3.5e-197
Identity = 344/346 (99.42%), Postives = 345/346 (99.71%), Query Frame = 0


Query: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 60
           MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK
Sbjct: 32  MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 91

Query: 61  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120
           ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL
Sbjct: 92  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 151

Query: 121 PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA 180
           PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA
Sbjct: 152 PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA 211

Query: 181 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGG 240
           AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSG 
Sbjct: 212 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGS 271

Query: 241 KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 300
           KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLE+FTSFNGPDF
Sbjct: 272 KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLESFTSFNGPDF 331

Query: 301 YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN 347
           YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN
Sbjct: 332 YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN 377

[Close]

BLAST of CSPI03G38820.2 vs. ExPASy TrEMBL
Match: A0A1S3B8N1 (Dihydroorotase OS=Cucumis melo OX=3656 GN=LOC103486989 PE=3 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 7.2e-195
Identity = 338/346 (97.69%), Postives = 343/346 (99.13%), Query Frame = 0


Query: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 60
           MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPP+TTTAAAVAYRDSILK
Sbjct: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPITTTAAAVAYRDSILK 60

Query: 61  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120
           ALPSNSNF+PLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL
Sbjct: 61  ALPSNSNFIPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120

Query: 121 PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA 180
           PVLEEMVEQDMPLLVHGEVTN DVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTA+A
Sbjct: 121 PVLEEMVEQDMPLLVHGEVTNPDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTANA 180

Query: 181 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGG 240
           AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSG 
Sbjct: 181 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGS 240

Query: 241 KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 300
           KKFFLGTDSAPHER+RKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF
Sbjct: 241 KKFFLGTDSAPHERRRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 300

Query: 301 YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN 347
           YGLPRNTSKITLQKAPWQVPKSF+F FGDIVPMFAGETLEWQPCFN
Sbjct: 301 YGLPRNTSKITLQKAPWQVPKSFTFPFGDIVPMFAGETLEWQPCFN 346

[Close]

BLAST of CSPI03G38820.2 vs. ExPASy TrEMBL
Match: A0A5D3DPM6 (Dihydroorotase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007860 PE=3 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 7.2e-195
Identity = 338/346 (97.69%), Postives = 343/346 (99.13%), Query Frame = 0


Query: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 60
           MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPP+TTTAAAVAYRDSILK
Sbjct: 119 MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPITTTAAAVAYRDSILK 178

Query: 61  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120
           ALPSNSNF+PLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL
Sbjct: 179 ALPSNSNFIPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 238

Query: 121 PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA 180
           PVLEEMVEQDMPLLVHGEVTN DVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTA+A
Sbjct: 239 PVLEEMVEQDMPLLVHGEVTNPDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTANA 298

Query: 181 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGG 240
           AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSG 
Sbjct: 299 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGS 358

Query: 241 KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 300
           KKFFLGTDSAPHER+RKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF
Sbjct: 359 KKFFLGTDSAPHERRRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 418

Query: 301 YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN 347
           YGLPRNTSKITLQKAPWQVPKSF+F FGDIVPMFAGETLEWQPCFN
Sbjct: 419 YGLPRNTSKITLQKAPWQVPKSFTFPFGDIVPMFAGETLEWQPCFN 464

[Close]

BLAST of CSPI03G38820.2 vs. ExPASy TrEMBL
Match: A0A1S3B7W2 (Dihydroorotase OS=Cucumis melo OX=3656 GN=LOC103486989 PE=3 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 7.2e-195
Identity = 338/346 (97.69%), Postives = 343/346 (99.13%), Query Frame = 0


Query: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 60
           MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPP+TTTAAAVAYRDSILK
Sbjct: 32  MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPITTTAAAVAYRDSILK 91

Query: 61  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120
           ALPSNSNF+PLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL
Sbjct: 92  ALPSNSNFIPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 151

Query: 121 PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA 180
           PVLEEMVEQDMPLLVHGEVTN DVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTA+A
Sbjct: 152 PVLEEMVEQDMPLLVHGEVTNPDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTANA 211

Query: 181 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGG 240
           AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSG 
Sbjct: 212 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGS 271

Query: 241 KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 300
           KKFFLGTDSAPHER+RKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF
Sbjct: 272 KKFFLGTDSAPHERRRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 331

Query: 301 YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN 347
           YGLPRNTSKITLQKAPWQVPKSF+F FGDIVPMFAGETLEWQPCFN
Sbjct: 332 YGLPRNTSKITLQKAPWQVPKSFTFPFGDIVPMFAGETLEWQPCFN 377

[Close]

BLAST of CSPI03G38820.2 vs. ExPASy TrEMBL
Match: A0A5A7UFB1 (Dihydroorotase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G001630 PE=3 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 1.8e-193
Identity = 338/347 (97.41%), Postives = 343/347 (98.85%), Query Frame = 0


Query: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 60
           MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPP+TTTAAAVAYRDSILK
Sbjct: 119 MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPITTTAAAVAYRDSILK 178

Query: 61  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120
           ALPSNSNF+PLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL
Sbjct: 179 ALPSNSNFIPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 238

Query: 121 PVLEEMVEQDMPLL-VHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTAD 180
           PVLEEMVEQDMPLL VHGEVTN DVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTA+
Sbjct: 239 PVLEEMVEQDMPLLVVHGEVTNPDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTAN 298

Query: 181 AAKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSG 240
           AAKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSG
Sbjct: 299 AAKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSG 358

Query: 241 GKKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPD 300
            KKFFLGTDSAPHER+RKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPD
Sbjct: 359 SKKFFLGTDSAPHERRRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPD 418

Query: 301 FYGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN 347
           FYGLPRNTSKITLQKAPWQVPKSF+F FGDIVPMFAGETLEWQPCFN
Sbjct: 419 FYGLPRNTSKITLQKAPWQVPKSFTFPFGDIVPMFAGETLEWQPCFN 465

[Close]

BLAST of CSPI03G38820.2 vs. NCBI nr
Match: XP_011652231.1 (dihydroorotase, mitochondrial isoform X2 [Cucumis sativus] >XP_031737524.1 dihydroorotase, mitochondrial isoform X2 [Cucumis sativus])

HSP 1 Score: 697.2 bits (1798), Expect = 7.2e-197
Identity = 344/346 (99.42%), Postives = 345/346 (99.71%), Query Frame = 0


Query: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 60
           MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK
Sbjct: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 60

Query: 61  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120
           ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL
Sbjct: 61  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120

Query: 121 PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA 180
           PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA
Sbjct: 121 PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA 180

Query: 181 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGG 240
           AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSG 
Sbjct: 181 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGS 240

Query: 241 KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 300
           KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLE+FTSFNGPDF
Sbjct: 241 KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLESFTSFNGPDF 300

Query: 301 YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN 347
           YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN
Sbjct: 301 YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN 346

[Close]

BLAST of CSPI03G38820.2 vs. NCBI nr
Match: XP_004136697.2 (dihydroorotase, mitochondrial isoform X1 [Cucumis sativus] >KGN59552.1 hypothetical protein Csa_002648 [Cucumis sativus])

HSP 1 Score: 697.2 bits (1798), Expect = 7.2e-197
Identity = 344/346 (99.42%), Postives = 345/346 (99.71%), Query Frame = 0


Query: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 60
           MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK
Sbjct: 32  MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 91

Query: 61  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120
           ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL
Sbjct: 92  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 151

Query: 121 PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA 180
           PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA
Sbjct: 152 PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA 211

Query: 181 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGG 240
           AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSG 
Sbjct: 212 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGS 271

Query: 241 KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 300
           KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLE+FTSFNGPDF
Sbjct: 272 KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLESFTSFNGPDF 331

Query: 301 YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN 347
           YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN
Sbjct: 332 YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN 377

[Close]

BLAST of CSPI03G38820.2 vs. NCBI nr
Match: XP_008443395.1 (PREDICTED: dihydroorotase, mitochondrial isoform X2 [Cucumis melo] >XP_008443396.1 PREDICTED: dihydroorotase, mitochondrial isoform X2 [Cucumis melo])

HSP 1 Score: 689.5 bits (1778), Expect = 1.5e-194
Identity = 338/346 (97.69%), Postives = 343/346 (99.13%), Query Frame = 0


Query: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 60
           MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPP+TTTAAAVAYRDSILK
Sbjct: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPITTTAAAVAYRDSILK 60

Query: 61  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120
           ALPSNSNF+PLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL
Sbjct: 61  ALPSNSNFIPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120

Query: 121 PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA 180
           PVLEEMVEQDMPLLVHGEVTN DVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTA+A
Sbjct: 121 PVLEEMVEQDMPLLVHGEVTNPDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTANA 180

Query: 181 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGG 240
           AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSG 
Sbjct: 181 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGS 240

Query: 241 KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 300
           KKFFLGTDSAPHER+RKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF
Sbjct: 241 KKFFLGTDSAPHERRRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 300

Query: 301 YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN 347
           YGLPRNTSKITLQKAPWQVPKSF+F FGDIVPMFAGETLEWQPCFN
Sbjct: 301 YGLPRNTSKITLQKAPWQVPKSFTFPFGDIVPMFAGETLEWQPCFN 346

[Close]

BLAST of CSPI03G38820.2 vs. NCBI nr
Match: TYK25626.1 (dihydroorotase [Cucumis melo var. makuwa])

HSP 1 Score: 689.5 bits (1778), Expect = 1.5e-194
Identity = 338/346 (97.69%), Postives = 343/346 (99.13%), Query Frame = 0


Query: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 60
           MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPP+TTTAAAVAYRDSILK
Sbjct: 119 MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPITTTAAAVAYRDSILK 178

Query: 61  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120
           ALPSNSNF+PLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL
Sbjct: 179 ALPSNSNFIPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 238

Query: 121 PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA 180
           PVLEEMVEQDMPLLVHGEVTN DVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTA+A
Sbjct: 239 PVLEEMVEQDMPLLVHGEVTNPDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTANA 298

Query: 181 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGG 240
           AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSG 
Sbjct: 299 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGS 358

Query: 241 KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 300
           KKFFLGTDSAPHER+RKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF
Sbjct: 359 KKFFLGTDSAPHERRRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 418

Query: 301 YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN 347
           YGLPRNTSKITLQKAPWQVPKSF+F FGDIVPMFAGETLEWQPCFN
Sbjct: 419 YGLPRNTSKITLQKAPWQVPKSFTFPFGDIVPMFAGETLEWQPCFN 464

[Close]

BLAST of CSPI03G38820.2 vs. NCBI nr
Match: XP_008443394.1 (PREDICTED: dihydroorotase, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 689.5 bits (1778), Expect = 1.5e-194
Identity = 338/346 (97.69%), Postives = 343/346 (99.13%), Query Frame = 0


Query: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 60
           MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPP+TTTAAAVAYRDSILK
Sbjct: 32  MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPITTTAAAVAYRDSILK 91

Query: 61  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120
           ALPSNSNF+PLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL
Sbjct: 92  ALPSNSNFIPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 151

Query: 121 PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA 180
           PVLEEMVEQDMPLLVHGEVTN DVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTA+A
Sbjct: 152 PVLEEMVEQDMPLLVHGEVTNPDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTANA 211

Query: 181 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGG 240
           AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSG 
Sbjct: 212 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGS 271

Query: 241 KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 300
           KKFFLGTDSAPHER+RKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF
Sbjct: 272 KKFFLGTDSAPHERRRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 331

Query: 301 YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQPCFN 347
           YGLPRNTSKITLQKAPWQVPKSF+F FGDIVPMFAGETLEWQPCFN
Sbjct: 332 YGLPRNTSKITLQKAPWQVPKSFTFPFGDIVPMFAGETLEWQPCFN 377

[Close]

BLAST of CSPI03G38820.2 vs. TAIR 10
Match: AT4G22930.1 (pyrimidin 4 )

HSP 1 Score: 588.6 bits (1516), Expect = 3.3e-168
Identity = 283/343 (82.51%), Postives = 313/343 (91.25%), Query Frame = 0


Query: 1   MQLTLTQPDDWHLHLRDGDLLKAVLPHSASTFGRAIVMPNLKPPVTTTAAAVAYRDSILK 60
           M+LT+TQPDDWHLHLRDGDLL AV+PHSAS F RAIVMPNLKPPVT+TAAA+ YR  I+K
Sbjct: 33  MELTITQPDDWHLHLRDGDLLHAVVPHSASNFKRAIVMPNLKPPVTSTAAAIIYRKFIMK 92

Query: 61  ALPSNSNFVPLMTLYLTDTMSPNEIKLARKSGAVYAVKLYPAGATTNSQDGVTDLFGKCL 120
           ALPS S+F PLMTLYLTD   P EI+LAR+SG VYAVKLYPAGATTNSQDGVTDLFGKCL
Sbjct: 93  ALPSESSFDPLMTLYLTDKTLPEEIRLARESGVVYAVKLYPAGATTNSQDGVTDLFGKCL 152

Query: 121 PVLEEMVEQDMPLLVHGEVTNSDVDMFDREKVFIETVLKPLIERLPKLKVVMEHITTADA 180
           PVLEEMV+Q+MPLLVHGEVT+  +D+FDREK+FIETVL+PLI+RLP+LKVVMEHITT DA
Sbjct: 153 PVLEEMVKQNMPLLVHGEVTDPSIDVFDREKIFIETVLQPLIQRLPQLKVVMEHITTMDA 212

Query: 181 AKFVLSCEHALLAATVTPQHLLLNRNSLFQGGLQPHNYCLPVLKRETHRQAIVSAVTSGG 240
             FV SC+   + ATVTPQHLLLNRN+LFQGGLQPHNYCLPVLKRE HR+AIV AVTSG 
Sbjct: 213 VNFVESCKEGSVGATVTPQHLLLNRNALFQGGLQPHNYCLPVLKREIHREAIVKAVTSGS 272

Query: 241 KKFFLGTDSAPHERKRKESSCGCAGIYSAPIALSLYAKVFEEAGALDKLEAFTSFNGPDF 300
           KKFFLGTDSAPHER RKESSCGCAGIYSAPIALSLYAKVF+EAGALDKLEAFTSFNGPDF
Sbjct: 273 KKFFLGTDSAPHERSRKESSCGCAGIYSAPIALSLYAKVFDEAGALDKLEAFTSFNGPDF 332

Query: 301 YGLPRNTSKITLQKAPWQVPKSFSFSFGDIVPMFAGETLEWQP 344
           YGLPRN+SKITL+K+PW+VP  F+F FG+IVPMFAGETL+WQP
Sbjct: 333 YGLPRNSSKITLKKSPWKVPDVFNFPFGEIVPMFAGETLQWQP 375

The following BLAST results are available for this feature:

BLAST of CSPI03G38820.2 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04904	4.7e-167	82.51	Dihydroorotase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PYR4 PE=1 SV=1	[more]
Q0JJD1	4.6e-162	79.24	Dihydroorotase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=PYRC P...	[more]
B1XI96	1.8e-126	65.69	Dihydroorotase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) OX=320...	[more]
C1D672	4.2e-123	65.40	Dihydroorotase OS=Laribacter hongkongensis (strain HLHK9) OX=557598 GN=pyrC PE=3...	[more]
B0CF78	7.9e-122	63.64	Dihydroorotase OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=pyrC PE=...	[more]

BLAST of CSPI03G38820.2 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LG11	3.5e-197	99.42	Dihydroorotase OS=Cucumis sativus OX=3659 GN=Csa_3G824970 PE=3 SV=1	[more]
A0A1S3B8N1	7.2e-195	97.69	Dihydroorotase OS=Cucumis melo OX=3656 GN=LOC103486989 PE=3 SV=1	[more]
A0A5D3DPM6	7.2e-195	97.69	Dihydroorotase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00786...	[more]
A0A1S3B7W2	7.2e-195	97.69	Dihydroorotase OS=Cucumis melo OX=3656 GN=LOC103486989 PE=3 SV=1	[more]
A0A5A7UFB1	1.8e-193	97.41	Dihydroorotase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold135G00163...	[more]

BLAST of CSPI03G38820.2 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011652231.1	7.2e-197	99.42	dihydroorotase, mitochondrial isoform X2 [Cucumis sativus] >XP_031737524.1 dihyd...	[more]
XP_004136697.2	7.2e-197	99.42	dihydroorotase, mitochondrial isoform X1 [Cucumis sativus] >KGN59552.1 hypotheti...	[more]
XP_008443395.1	1.5e-194	97.69	PREDICTED: dihydroorotase, mitochondrial isoform X2 [Cucumis melo] >XP_008443396...	[more]
TYK25626.1	1.5e-194	97.69	dihydroorotase [Cucumis melo var. makuwa]	[more]
XP_008443394.1	1.5e-194	97.69	PREDICTED: dihydroorotase, mitochondrial isoform X1 [Cucumis melo]	[more]

BLAST of CSPI03G38820.2 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G22930.1	3.3e-168	82.51	pyrimidin 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006680	Amidohydrolase-related	PFAM	PF01979	Amidohydro_1	coord: 10..319 e-value: 3.5E-27 score: 95.6
IPR004721	Dihydroorotase homodimeric type	TIGRFAM	TIGR00856	TIGR00856	coord: 3..342 e-value: 1.7E-145 score: 482.3
IPR004721	Dihydroorotase homodimeric type	PIRSF	PIRSF001237	Dihydroorotase	coord: 1..345 e-value: 5.8E-146 score: 483.8
IPR004721	Dihydroorotase homodimeric type	PANTHER	PTHR43137	DIHYDROOROTASE	coord: 1..344
IPR004721	Dihydroorotase homodimeric type	HAMAP	MF_00219	PyrC_classII	coord: 1..344 score: 47.12632
IPR004721	Dihydroorotase homodimeric type	CDD	cd01294	DHOase	coord: 3..341 e-value: 0.0 score: 568.452
None	No IPR available	GENE3D	3.20.20.140		coord: 1..343 e-value: 1.2E-126 score: 424.5
IPR002195	Dihydroorotase, conserved site	PROSITE	PS00483	DIHYDROOROTASE_2	coord: 246..257
IPR002195	Dihydroorotase, conserved site	PROSITE	PS00482	DIHYDROOROTASE_1	coord: 10..18
IPR032466	Metal-dependent hydrolase	SUPERFAMILY	51556	Metallo-dependent hydrolases	coord: 2..342

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G38820	CSPI03G38820	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G38820.2.utr3p1	CSPI03G38820.2.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G38820.2.cds8	CSPI03G38820.2.cds8	CDS
CSPI03G38820.2.cds7	CSPI03G38820.2.cds7	CDS
CSPI03G38820.2.cds6	CSPI03G38820.2.cds6	CDS
CSPI03G38820.2.cds5	CSPI03G38820.2.cds5	CDS
CSPI03G38820.2.cds4	CSPI03G38820.2.cds4	CDS
CSPI03G38820.2.cds3	CSPI03G38820.2.cds3	CDS
CSPI03G38820.2.cds2	CSPI03G38820.2.cds2	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G38820.2.utr5p2	CSPI03G38820.2.utr5p2	five_prime_UTR
CSPI03G38820.2.utr5p1	CSPI03G38820.2.utr5p1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G38820.2	CSPI03G38820.2-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019856	pyrimidine nucleobase biosynthetic process
biological_process	GO:0006207	'de novo' pyrimidine nucleobase biosynthetic process
biological_process	GO:0044205	'de novo' UMP biosynthetic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004151	dihydroorotase activity
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0016812	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides
molecular_function	GO:0046872	metal ion binding