CSPI03G38080.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI03G38080.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RING-type E3 ubiquitin transferase
Location	Chr3: 32968424 .. 32971373 (+)
Sequence length	1622
RNA-Seq Expression	CSPI03G38080.1
Synteny	CSPI03G38080.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAAAACATCCCAAAAACTTTTTGTAATGGAAAAACGTGTCCTTCAAATACCAAAGTGTTGGATTTATTGAAAGTCCAGCTTCGTGCTTCTTCTCTACACTCAAAAGGATAGAGAAAACCCAAAAAAAAAAGAAAAAAAACTTTGGGAATAAATTCCATTTTCTTCACTCTCCATCTCCATCTTCATCATCCCCCATCTTTAGGGTTCCCTTCCCATCTTCTTCCTTCTCTTTACCATCACTTCACATTCACAAAGGTGCGTTTTTTTTTTTTCCCATTTCAATTTCTTGCTTTCTGTACTTCTCTATTCCCTCTGTTTCTCCATTCTTCACTGTGTTCCATTGCCCGCTTTCCACTTTTTATCTGATGGGGCTCATTCTGTTTTACATTTTAATTTAATTGTTCTCAAGGTGTAGCCAATGCTTGCAAATTTAAGTTGAATTTCCTGTTAATTTCTGATTTTTATTCTCTTTTTTTTCTTTCTTTCTTTTGACTTGATGTTGTTTCAACTAACTTCATTGACTTATTTATTCGTTTGTTGAAGTGGGAAAATTGATTCCTGAAGTTGGTTTCACTTTTATGGTTTGATAGTGCTATTTTTCATACTTGAACAGTGTCACTGGATCTAGGATCGATATGTGTCTCATATGGTACAAACCCATTTCTATGCTATTGTTGGAGTATTGATATGATTAAATTTAACATAATCCATTAGCTCTAGTGTTTGGATTGATGGATGTCATTTTTTCCTCAATTAATTCTGATTTCTATGTTGGATCTAGTGAAGCAGTGTTGAAGTATTGGTATAATTAAATTTACTATAACCCATTAACTTAAGCTTTTGGATTTATTAGTGATTTAAAAATTAAGATCTAAAATGATAATGATTCATATTGCTACTTTGGTGATTATCTTAGAGAAGTATGCTTTGCTTAAGTTCTTGGACTGATACTCCTTGGAATTACCGAATGGAAAATTAGACATTTTCTAGAGCTTGATTTTATTACGAACTATTACTCCAATGTTCCAAAACATTTTTGAGGTGAAGTCGTTCTTACACTAGCCTATCCAACCAATAGGATGCTCTCTCGCACATTGAACTTCCAAATGCTTCTTCAAGCCCCAGAAAATGTACTCTTACTGATATATATTCCTAGATATGTGGATGCGCATCATCCAATACATATTCATGATCGAAATAGAAGCAATTTGGGTTTGAAATGTTTAAACTGCAGCTCTTTTGAAACCATCATTGGCGGCCTAGTGGTAAATAAAAATCTTGGGTTTGATGAATGACTTAAGAGGTTAATTGATCTAATCTAGAATCTGTTATTTCAGAGCATAGTTCTCAAACTTTTGCATCTTGTGATAAATGAAACATGCATATGATGTGAAAGTACTTATTTTGTGCTGATTAATTAAATCCAGAAGTAAAAGGACAAAAGTCACCCAAGTGTTTTCTTTGTCAGCTGAGTACATAACTCAGGCTTGAGATTATTAAACTTGAAAGTGACAAGATTTATATGTAGCAAGCTAACAATTCAATACGTAAAGAATGAGGTATTTCACGGTTGAGACTTGCAGGGAAGAGAAGAAGAATGTCGACTGCAAACACCCATTGGTGTTACAGATGCGAACAGCCAGTTCGACTTCGTGGGCGAGACATGACTTGCCTTAGCTGTAATGGAGGATTTGTTCAAGAACTGGATGAAATGATGGAAAGAAGTCCCCTTGATTTATTTGGAGCCAGTGGTAATGAATACCAAAACCGCAGGCTAGGACTCTTGGAGCTTTTCTCAAACTTTATGAGGCAACGATTGGTAGACAGAAATGATATCAGGGGAAGGTTGGACTCAATTCCAGATCATGGTCCAGGATTTGGTCCTTGGTTGATCTTTGGTGGCCAAATTCCTGTTAGATTGTCTGGACATGGTGGATTTGAAGCGTTTTTTAATGGGGTACCTGGCATTGGTGTGAGTAGGAGCAATGGTGGTGATTATTTTATTGGTCCTGGACTCGAAGAATTGTTTGAGCAGCTTTCAGCAAACGATCGACGAGGGCCACCACCGGCATCAAGATCTTCGATTGATGCAATGCCTGTTGTTAAAATTACACAAAGACACATTCGTTCCAATTCACACTGTCCTGTCTGCCAAGATAAATTTGAATTAGGATCTGAGGCTAGGCAAATGCCATGTGACCATATGTATCATTCAGATTGTATTGTTCCATGGCTAGTCCAGCACAACTCCTGCCCTGTTTGCCGCCAAGAACTGCCTGCACAAGGGTCGGGTAGTAGCCATAGCTCATCTGGAAGCAACAATTCTAGTCGAAACAGCCGAGATAGGGAGAATGGTAGAACGACTCAAGGAAGGAGGAATCCATTTACAAGTCTTTGGCCATTCCGAGCATCAAGCTCGAACTCTAACCATAGTGCTACCCCTGGAAGCAACTCACCAGCTCTACACGAGACTAACCAACACGAAACAGGGTACTACGGATGGCCTTTCGACTAGCATCAACACTGCGTTCCTAAACTTGTTTGCAGCATTCAGTTTTTGTGATTGTTATTTAACATGATGAAAATAGTTCAAGCCCTGCAGTCCACTCTCTTGTATAATAAATTGATAATAATGGACTTCCTGTGTGATGTAGACGACATTTTTCTATTCCTTATCGATGCTGCTGGAATTTGTATATGCTTCATGTATATTAGCATGGCGTTTCTTTCTAGAGTTGCAGGTGGTCGGGAAATCTGTCTGTTCAAGTGAGGATTGCTGAATCTTCTTCAACTGAAAGTTAAAACGTCCAAATCAAGCTATTCTAGTATCTACTCAAATTGACCAATGTTTTCCTTATGCATCATCATTCATACTAAAACTGTACATGTTTTTCTAAAAGTAGAGGACAACATATTTTAGCATTTAAGAAATCTTTCAAGATGGATTGA

mRNA sequence

AGAAAACATCCCAAAAACTTTTTGTAATGGAAAAACGTGTCCTTCAAATACCAAAGTGTTGGATTTATTGAAAGTCCAGCTTCGTGCTTCTTCTCTACACTCAAAAGGATAGAGAAAACCCAAAAAAAAAAGAAAAAAAACTTTGGGAATAAATTCCATTTTCTTCACTCTCCATCTCCATCTTCATCATCCCCCATCTTTAGGGTTCCCTTCCCATCTTCTTCCTTCTCTTTACCATCACTTCACATTCACAAAGGGAAGAGAAGAAGAATGTCGACTGCAAACACCCATTGGTGTTACAGATGCGAACAGCCAGTTCGACTTCGTGGGCGAGACATGACTTGCCTTAGCTGTAATGGAGGATTTGTTCAAGAACTGGATGAAATGATGGAAAGAAGTCCCCTTGATTTATTTGGAGCCAGTGGTAATGAATACCAAAACCGCAGGCTAGGACTCTTGGAGCTTTTCTCAAACTTTATGAGGCAACGATTGGTAGACAGAAATGATATCAGGGGAAGGTTGGACTCAATTCCAGATCATGGTCCAGGATTTGGTCCTTGGTTGATCTTTGGTGGCCAAATTCCTGTTAGATTGTCTGGACATGGTGGATTTGAAGCGTTTTTTAATGGGGTACCTGGCATTGGTGTGAGTAGGAGCAATGGTGGTGATTATTTTATTGGTCCTGGACTCGAAGAATTGTTTGAGCAGCTTTCAGCAAACGATCGACGAGGGCCACCACCGGCATCAAGATCTTCGATTGATGCAATGCCTGTTGTTAAAATTACACAAAGACACATTCGTTCCAATTCACACTGTCCTGTCTGCCAAGATAAATTTGAATTAGGATCTGAGGCTAGGCAAATGCCATGTGACCATATGTATCATTCAGATTGTATTGTTCCATGGCTAGTCCAGCACAACTCCTGCCCTGTTTGCCGCCAAGAACTGCCTGCACAAGGGTCGGGTAGTAGCCATAGCTCATCTGGAAGCAACAATTCTAGTCGAAACAGCCGAGATAGGGAGAATGGTAGAACGACTCAAGGAAGGAGGAATCCATTTACAAGTCTTTGGCCATTCCGAGCATCAAGCTCGAACTCTAACCATAGTGCTACCCCTGGAAGCAACTCACCAGCTCTACACGAGACTAACCAACACGAAACAGGGTACTACGGATGGCCTTTCGACTAGCATCAACACTGCGTTCCTAAACTTGTTTGCAGCATTCAGTTTTTGTGATTGTTATTTAACATGATGAAAATAGTTCAAGCCCTGCAGTCCACTCTCTTGTATAATAAATTGATAATAATGGACTTCCTGTGTGATGTAGACGACATTTTTCTATTCCTTATCGATGCTGCTGGAATTTGTATATGCTTCATGTATATTAGCATGGCGTTTCTTTCTAGAGTTGCAGGTGGTCGGGAAATCTGTCTGTTCAAGTGAGGATTGCTGAATCTTCTTCAACTGAAAGTTAAAACGTCCAAATCAAGCTATTCTAGTATCTACTCAAATTGACCAATGTTTTCCTTATGCATCATCATTCATACTAAAACTGTACATGTTTTTCTAAAAGTAGAGGACAACATATTTTAGCATTTAAGAAATCTTTCAAGATGGATTGA

Coding sequence (CDS)

ATGTCGACTGCAAACACCCATTGGTGTTACAGATGCGAACAGCCAGTTCGACTTCGTGGGCGAGACATGACTTGCCTTAGCTGTAATGGAGGATTTGTTCAAGAACTGGATGAAATGATGGAAAGAAGTCCCCTTGATTTATTTGGAGCCAGTGGTAATGAATACCAAAACCGCAGGCTAGGACTCTTGGAGCTTTTCTCAAACTTTATGAGGCAACGATTGGTAGACAGAAATGATATCAGGGGAAGGTTGGACTCAATTCCAGATCATGGTCCAGGATTTGGTCCTTGGTTGATCTTTGGTGGCCAAATTCCTGTTAGATTGTCTGGACATGGTGGATTTGAAGCGTTTTTTAATGGGGTACCTGGCATTGGTGTGAGTAGGAGCAATGGTGGTGATTATTTTATTGGTCCTGGACTCGAAGAATTGTTTGAGCAGCTTTCAGCAAACGATCGACGAGGGCCACCACCGGCATCAAGATCTTCGATTGATGCAATGCCTGTTGTTAAAATTACACAAAGACACATTCGTTCCAATTCACACTGTCCTGTCTGCCAAGATAAATTTGAATTAGGATCTGAGGCTAGGCAAATGCCATGTGACCATATGTATCATTCAGATTGTATTGTTCCATGGCTAGTCCAGCACAACTCCTGCCCTGTTTGCCGCCAAGAACTGCCTGCACAAGGGTCGGGTAGTAGCCATAGCTCATCTGGAAGCAACAATTCTAGTCGAAACAGCCGAGATAGGGAGAATGGTAGAACGACTCAAGGAAGGAGGAATCCATTTACAAGTCTTTGGCCATTCCGAGCATCAAGCTCGAACTCTAACCATAGTGCTACCCCTGGAAGCAACTCACCAGCTCTACACGAGACTAACCAACACGAAACAGGGTACTACGGATGGCCTTTCGACTAG

Protein sequence

MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRLGLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNGVPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNSHCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGSNNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPALHETNQHETGYYGWPFD*

Homology

[Close]

BLAST of CSPI03G38080.1 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 7.0e-90
Identity = 183/329 (55.62%), Postives = 231/329 (70.21%), Query Frame = 0


Query: 2   STANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGA-SGNEYQNRRL 61
           S+ NTHWC+RC++ VRL G++  C  C GGFV+ELD M + SP D+F +  G   +++  
Sbjct: 3   SSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELD-MAQASPFDMFRSHRGVVERDQTF 62

Query: 62  GLLELFSNFMRQRLVDRN---DIRGR-LDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEA 121
            L++ FS FMR RL +R+   +IRGR + S P++ PG  P LIFGGQ+P RL+G    EA
Sbjct: 63  DLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVEA 122

Query: 122 FFN-GVPGIGVSRSNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQR 181
            FN G PGIG++R N GDYF GPGLEELFEQLSA   RRGPPPA RS+IDA+P +KI QR
Sbjct: 123 LFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQR 182

Query: 182 HIR-SNSHCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELP-AQGSG 241
           H+R S+S+CPVC+D+FELGSEA+QMPC+H+YHSDCIVPWLVQHNSCPVCRQELP A G  
Sbjct: 183 HLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPS 242

Query: 242 SSHSSS------GSNNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGS-- 301
           SS + +       S++SS +S  RENG     RRNPF+S WPFR+S S+S+ +   G   
Sbjct: 243 SSQNRTTPTRNYRSSSSSSSSNSRENGNE---RRNPFSSFWPFRSSGSSSSSTQNRGGTR 302

Query: 302 NSPALHETN-----QHE---TGYYGWPFD 306
           NS    E +     QH+    GY GWPFD
Sbjct: 303 NSDTSDENHNYHQQQHQQSYMGYSGWPFD 327

[Close]

BLAST of CSPI03G38080.1 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 8.6e-80
Identity = 168/332 (50.60%), Postives = 212/332 (63.86%), Query Frame = 0


Query: 2   STANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRLG 61
           S  NTHWC+RC++ V LR RD  C  C GGFV+E+D    R+  D+        ++    
Sbjct: 3   SIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSRAHRDV-------ERDPTFD 62

Query: 62  LLELFSNFMRQRLVDRN---DIRGRLDSIPDHG-PGFGPWLIFGGQIPVRLSG--HGGFE 121
           L+E FS FMR RL +R+   +I GRL S          P LIFGGQ P RL+G  +   E
Sbjct: 63  LMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVE 122

Query: 122 AFFNG-VPGIGVSR-SNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKIT 181
           AF NG  PGIG++R +N GDYF GPGLEEL EQLS+    RGPPPA +SSIDA+P +KIT
Sbjct: 123 AFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKIT 182

Query: 182 QRHIR-SNSHCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS 241
           Q+H++ S+SHCPVC+D+FEL SEA+QMPC H+YHSDCIVPWLVQHNSCPVCR+ELP++GS
Sbjct: 183 QKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGS 242

Query: 242 GSSHSSSGSNNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPAL-- 301
            SS  SS     +R++  REN R    RRN F++LWPFR+SSS+S  +    +N+     
Sbjct: 243 SSSTQSS----QNRSTNGRENSR----RRNIFSNLWPFRSSSSSSTQNRRDTNNTATAEE 302

Query: 302 ----------------HETNQHETGYYGWPFD 306
                           H+  Q   GY GWPFD
Sbjct: 303 GHYHHHQQQQQQHQHQHQQQQSHMGYSGWPFD 319

[Close]

BLAST of CSPI03G38080.1 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 2.0e-36
Identity = 100/304 (32.89%), Postives = 146/304 (48.03%), Query Frame = 0


Query: 8   WCYRCEQPVRL---RGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRLGLLE 67
           +CY+C Q V +      D  C  CN GF++E ++      L+    S + +      + +
Sbjct: 22  FCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSSDSF----FPMAD 81

Query: 68  LFSNFMRQRLVDRNDIRGRLDSIPDHGP----------------GFGPWLIFGGQIPVRL 127
            FS  +             +D +   GP                 F P+      +    
Sbjct: 82  PFSTLLPLIFGSSAAAPSGMDFMSLFGPSMQPQARSTQQNPQSDAFDPFTFLQNHLQTLR 141

Query: 128 SGHGGFEAFFNGVPGIGVSR--SNGGDYFIGPGLEELFEQLSAND--RRGPPPASRSSID 187
           S    FE      P    +R   N GDYF GPGLE+L +QL+ ND  R G PPAS+S+ID
Sbjct: 142 SSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAID 201

Query: 188 AMPVVKITQRHIRSN-SHCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCR 247
           A+P VK+T+  ++S  + C VC D+FE GS+ +QMPC H++H DC++PWL  HNSCPVCR
Sbjct: 202 ALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCR 261

Query: 248 QELPAQGSGSSHSSSGSNNSSRNSRDRENGRTTQGRRNPFTSL---WPFRASSSNSNHSA 285
            ELP       + S GS  S       +   + +G++ P  S+   WPFR    + + S 
Sbjct: 262 FELPTDDPDYENRSQGSQGSG------DGQGSVEGQQTPRFSIQLPWPFRRQDGSGSGSG 315

[Close]

BLAST of CSPI03G38080.1 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 8.7e-32
Identity = 77/173 (44.51%), Postives = 105/173 (60.69%), Query Frame = 0


Query: 129 SNGGDYFIGPGLEELFEQLSAND--RRGPPPASRSSIDAMPVVKITQRHIRSN-SHCPVC 188
           +N GDYFIGPGLE+L +QL+ ND  R G PPAS+S+I+A+P+V IT+ ++ S  + C VC
Sbjct: 168 ANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVC 227

Query: 189 QDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQG-------SGSSHSSS 248
            D FE G+EA+QMPC H+YH DC++PWL  HNSCPVCR ELP           G+  +S 
Sbjct: 228 MDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPDYERRVRGAQGTSG 287

Query: 249 GSNNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPALHE 292
           G++  +   R   + RT + R    +  WPF+A          PG N+    E
Sbjct: 288 GNDGDNSGQRSDGDNRTVE-RSFRISLPWPFQARGP----GPAPGDNAETRQE 335

[Close]

BLAST of CSPI03G38080.1 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 6.9e-29
Identity = 85/260 (32.69%), Postives = 129/260 (49.62%), Query Frame = 0


Query: 6   THWCYRCEQPVRLRGRDMT-------CLSCNGGFVQELDEMMERSPLDLFGASGNEYQNR 65
           ++WCY C + V +     T       C  C+GGF++++++    +      AS       
Sbjct: 17  SYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQINDSSSAATELTIPAS------- 76

Query: 66  RLGLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGG----- 125
                E+ S    +R V R    GR        P F P ++  G    R  G  G     
Sbjct: 77  ----TEVRSINNNRRSVIRRRRSGR-------RPSFNPVIVLQGGAGEREEGEEGDAARD 136

Query: 126 ---FEAFFNGVPGIGVS--RSNGGDYFIGPGLEELFEQLSAND-------RRGPPPASRS 185
              FE +++   G G+     +  +  +G G E L EQLS  +       R G PPAS+S
Sbjct: 137 RRAFEFYYDDGSGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASATGIGRSGNPPASKS 196

Query: 186 SIDAMPVVKITQRHIRSNSHCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPV 242
           +I+++P V+I+  HI S ++C VC + FE  +EAR+MPC H++H DCIVPWL   NSCPV
Sbjct: 197 AIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPV 256

[Close]

BLAST of CSPI03G38080.1 vs. ExPASy TrEMBL
Match: A0A0A0LCH6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G822250 PE=4 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.2e-179
Identity = 304/305 (99.67%), Postives = 304/305 (99.67%), Query Frame = 0


Query: 1   MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60
           MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL
Sbjct: 1   MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS 240
           HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS 240

Query: 241 NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPALHETNQHETGYY 300
           NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSAT GSNSPALHETNQHETGYY
Sbjct: 241 NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATTGSNSPALHETNQHETGYY 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of CSPI03G38080.1 vs. ExPASy TrEMBL
Match: A0A5A7UFI3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006970 PE=4 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 7.8e-177
Identity = 299/305 (98.03%), Postives = 300/305 (98.36%), Query Frame = 0


Query: 1   MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60
           MST NTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL
Sbjct: 1   MSTGNTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQR+VDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRMVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS 240
           HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS SSHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240

Query: 241 NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPALHETNQHETGYY 300
           NNSSRNSRDREN RTTQGRRNPFTSLWPFRASSSNSNH AT GSNSPALHETNQHETGYY
Sbjct: 241 NNSSRNSRDRENSRTTQGRRNPFTSLWPFRASSSNSNHGATTGSNSPALHETNQHETGYY 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of CSPI03G38080.1 vs. ExPASy TrEMBL
Match: A0A1S3B774 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486912 PE=4 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 7.8e-177
Identity = 299/305 (98.03%), Postives = 300/305 (98.36%), Query Frame = 0


Query: 1   MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60
           MST NTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL
Sbjct: 1   MSTGNTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQR+VDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRMVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS 240
           HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS SSHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240

Query: 241 NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPALHETNQHETGYY 300
           NNSSRNSRDREN RTTQGRRNPFTSLWPFRASSSNSNH AT GSNSPALHETNQHETGYY
Sbjct: 241 NNSSRNSRDRENSRTTQGRRNPFTSLWPFRASSSNSNHGATTGSNSPALHETNQHETGYY 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of CSPI03G38080.1 vs. ExPASy TrEMBL
Match: A0A6J1IB00 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111473444 PE=4 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 5.8e-164
Identity = 275/305 (90.16%), Postives = 290/305 (95.08%), Query Frame = 0


Query: 1   MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60
           MS+ NTHWCYRCEQPV+LRGRDM C SCNGGFVQELDEMMER PLD FGA G+EY NRRL
Sbjct: 1   MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGAHGHEYHNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLL+LFSNFMRQRL+DRND+RGRL+SIPDHG GFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLDLFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180
           VPGIGVSR+NGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRNNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS 240
           HCPVCQDKFELGSEAR+MPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSG SHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 240

Query: 241 NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPALHETNQHETGYY 300
           NN SRNSR+REN RT QGRRNPF+SLWPFRASSS+SNHSAT GS+SPALHE+ QH+TGY 
Sbjct: 241 NNLSRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPALHESTQHQTGYS 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of CSPI03G38080.1 vs. ExPASy TrEMBL
Match: A0A6J1FK80 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446055 PE=4 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 7.6e-164
Identity = 275/305 (90.16%), Postives = 290/305 (95.08%), Query Frame = 0


Query: 1   MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60
           MS+ NTHWCYRCEQPV+LRGRDM C SCNGGFVQELDEMMER PLD FGA+G+EY NRRL
Sbjct: 1   MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERDPLDFFGANGHEYHNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQRL+DRND+RGRL+SIPDHG GFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS 240
           HCPVCQDKFELG+EAR+MPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSG  HSSSGS
Sbjct: 181 HCPVCQDKFELGTEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGIHSSSGS 240

Query: 241 NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPALHETNQHETGYY 300
           NN SRNSR+REN RT QGRRNPF+SLWPFRASSS+SNHSAT GS+SPALHE+ QH+TGY 
Sbjct: 241 NNLSRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPALHESTQHQTGYS 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of CSPI03G38080.1 vs. NCBI nr
Match: XP_004136656.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >KGN59473.1 hypothetical protein Csa_001982 [Cucumis sativus])

HSP 1 Score: 637.9 bits (1644), Expect = 4.5e-179
Identity = 304/305 (99.67%), Postives = 304/305 (99.67%), Query Frame = 0


Query: 1   MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60
           MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL
Sbjct: 1   MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS 240
           HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS 240

Query: 241 NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPALHETNQHETGYY 300
           NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSAT GSNSPALHETNQHETGYY
Sbjct: 241 NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATTGSNSPALHETNQHETGYY 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of CSPI03G38080.1 vs. NCBI nr
Match: XP_008443292.1 (PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0053858.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa] >TYK25545.1 E3 ubiquitin-protein ligase RING1-like [Cucumis melo var. makuwa])

HSP 1 Score: 629.4 bits (1622), Expect = 1.6e-176
Identity = 299/305 (98.03%), Postives = 300/305 (98.36%), Query Frame = 0


Query: 1   MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60
           MST NTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL
Sbjct: 1   MSTGNTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQR+VDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRMVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS 240
           HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS SSHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240

Query: 241 NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPALHETNQHETGYY 300
           NNSSRNSRDREN RTTQGRRNPFTSLWPFRASSSNSNH AT GSNSPALHETNQHETGYY
Sbjct: 241 NNSSRNSRDRENSRTTQGRRNPFTSLWPFRASSSNSNHGATTGSNSPALHETNQHETGYY 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of CSPI03G38080.1 vs. NCBI nr
Match: XP_038904133.1 (probable E3 ubiquitin-protein ligase RHC1A isoform X1 [Benincasa hispida])

HSP 1 Score: 608.2 bits (1567), Expect = 3.9e-170
Identity = 287/305 (94.10%), Postives = 296/305 (97.05%), Query Frame = 0


Query: 1   MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60
           MST NTHWCYRCEQPVRLRGRDM C SCNGGFVQELDEMMERSP DLF ASGNEYQNRRL
Sbjct: 34  MSTGNTHWCYRCEQPVRLRGRDMICPSCNGGFVQELDEMMERSPFDLFEASGNEYQNRRL 93

Query: 61  GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQR+VDRND+RGRL+SIPDHGPGFGPWLIFGGQIPVR+SGHGGFEAFFNG
Sbjct: 94  GLLELFSNFMRQRMVDRNDMRGRLESIPDHGPGFGPWLIFGGQIPVRMSGHGGFEAFFNG 153

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 154 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 213

Query: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS 240
           HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS SSHSSSGS
Sbjct: 214 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSVSSHSSSGS 273

Query: 241 NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPALHETNQHETGYY 300
           NNSSRNSRDREN RT+QGRRNPFTS+WPFRASSSNSNH AT GS+SPALHE+NQH+TGYY
Sbjct: 274 NNSSRNSRDRENSRTSQGRRNPFTSIWPFRASSSNSNHGATTGSSSPALHESNQHQTGYY 333

Query: 301 GWPFD 306
           GWPFD
Sbjct: 334 GWPFD 338

[Close]

BLAST of CSPI03G38080.1 vs. NCBI nr
Match: XP_038904134.1 (probable E3 ubiquitin-protein ligase RHC1A isoform X2 [Benincasa hispida])

HSP 1 Score: 608.2 bits (1567), Expect = 3.9e-170
Identity = 287/305 (94.10%), Postives = 296/305 (97.05%), Query Frame = 0


Query: 1   MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60
           MST NTHWCYRCEQPVRLRGRDM C SCNGGFVQELDEMMERSP DLF ASGNEYQNRRL
Sbjct: 1   MSTGNTHWCYRCEQPVRLRGRDMICPSCNGGFVQELDEMMERSPFDLFEASGNEYQNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQR+VDRND+RGRL+SIPDHGPGFGPWLIFGGQIPVR+SGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRMVDRNDMRGRLESIPDHGPGFGPWLIFGGQIPVRMSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS 240
           HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS SSHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSVSSHSSSGS 240

Query: 241 NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPALHETNQHETGYY 300
           NNSSRNSRDREN RT+QGRRNPFTS+WPFRASSSNSNH AT GS+SPALHE+NQH+TGYY
Sbjct: 241 NNSSRNSRDRENSRTSQGRRNPFTSIWPFRASSSNSNHGATTGSSSPALHESNQHQTGYY 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of CSPI03G38080.1 vs. NCBI nr
Match: KAG7028452.1 (putative E3 ubiquitin-protein ligase RHC1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 589.7 bits (1519), Expect = 1.4e-164
Identity = 277/305 (90.82%), Postives = 291/305 (95.41%), Query Frame = 0


Query: 1   MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60
           MS+ NTHWCYRCEQPV+LRGRDM C SCNGGFVQELDEMMER PLD FGA+G+EY NRRL
Sbjct: 1   MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERDPLDFFGANGHEYHNRRL 60

Query: 61  GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120
           GLLELFSNFMRQRL+DRND+RGRL+SIPDHG GFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS 240
           HCPVCQDKFELGSEAR+MPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSG SHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 240

Query: 241 NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPALHETNQHETGYY 300
           NN SRNSR+REN RT QGRRNPF+SLWPFRASSS+SNHSAT GS+SPALHE+ QH+TGY 
Sbjct: 241 NNLSRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPALHESTQHQTGYS 300

Query: 301 GWPFD 306
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of CSPI03G38080.1 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 332.0 bits (850), Expect = 5.0e-91
Identity = 183/329 (55.62%), Postives = 231/329 (70.21%), Query Frame = 0


Query: 2   STANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGA-SGNEYQNRRL 61
           S+ NTHWC+RC++ VRL G++  C  C GGFV+ELD M + SP D+F +  G   +++  
Sbjct: 3   SSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELD-MAQASPFDMFRSHRGVVERDQTF 62

Query: 62  GLLELFSNFMRQRLVDRN---DIRGR-LDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEA 121
            L++ FS FMR RL +R+   +IRGR + S P++ PG  P LIFGGQ+P RL+G    EA
Sbjct: 63  DLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVEA 122

Query: 122 FFN-GVPGIGVSRSNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQR 181
            FN G PGIG++R N GDYF GPGLEELFEQLSA   RRGPPPA RS+IDA+P +KI QR
Sbjct: 123 LFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQR 182

Query: 182 HIR-SNSHCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELP-AQGSG 241
           H+R S+S+CPVC+D+FELGSEA+QMPC+H+YHSDCIVPWLVQHNSCPVCRQELP A G  
Sbjct: 183 HLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPS 242

Query: 242 SSHSSS------GSNNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGS-- 301
           SS + +       S++SS +S  RENG     RRNPF+S WPFR+S S+S+ +   G   
Sbjct: 243 SSQNRTTPTRNYRSSSSSSSSNSRENGNE---RRNPFSSFWPFRSSGSSSSSTQNRGGTR 302

Query: 302 NSPALHETN-----QHE---TGYYGWPFD 306
           NS    E +     QH+    GY GWPFD
Sbjct: 303 NSDTSDENHNYHQQQHQQSYMGYSGWPFD 327

[Close]

BLAST of CSPI03G38080.1 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 332.0 bits (850), Expect = 5.0e-91
Identity = 183/329 (55.62%), Postives = 231/329 (70.21%), Query Frame = 0


Query: 2   STANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGA-SGNEYQNRRL 61
           S+ NTHWC+RC++ VRL G++  C  C GGFV+ELD M + SP D+F +  G   +++  
Sbjct: 3   SSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELD-MAQASPFDMFRSHRGVVERDQTF 62

Query: 62  GLLELFSNFMRQRLVDRN---DIRGR-LDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEA 121
            L++ FS FMR RL +R+   +IRGR + S P++ PG  P LIFGGQ+P RL+G    EA
Sbjct: 63  DLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVEA 122

Query: 122 FFN-GVPGIGVSRSNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQR 181
            FN G PGIG++R N GDYF GPGLEELFEQLSA   RRGPPPA RS+IDA+P +KI QR
Sbjct: 123 LFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQR 182

Query: 182 HIR-SNSHCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELP-AQGSG 241
           H+R S+S+CPVC+D+FELGSEA+QMPC+H+YHSDCIVPWLVQHNSCPVCRQELP A G  
Sbjct: 183 HLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPS 242

Query: 242 SSHSSS------GSNNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGS-- 301
           SS + +       S++SS +S  RENG     RRNPF+S WPFR+S S+S+ +   G   
Sbjct: 243 SSQNRTTPTRNYRSSSSSSSSNSRENGNE---RRNPFSSFWPFRSSGSSSSSTQNRGGTR 302

Query: 302 NSPALHETN-----QHE---TGYYGWPFD 306
           NS    E +     QH+    GY GWPFD
Sbjct: 303 NSDTSDENHNYHQQQHQQSYMGYSGWPFD 327

[Close]

BLAST of CSPI03G38080.1 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 332.0 bits (850), Expect = 5.0e-91
Identity = 183/329 (55.62%), Postives = 231/329 (70.21%), Query Frame = 0


Query: 2   STANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGA-SGNEYQNRRL 61
           S+ NTHWC+RC++ VRL G++  C  C GGFV+ELD M + SP D+F +  G   +++  
Sbjct: 3   SSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELD-MAQASPFDMFRSHRGVVERDQTF 62

Query: 62  GLLELFSNFMRQRLVDRN---DIRGR-LDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEA 121
            L++ FS FMR RL +R+   +IRGR + S P++ PG  P LIFGGQ+P RL+G    EA
Sbjct: 63  DLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVEA 122

Query: 122 FFN-GVPGIGVSRSNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQR 181
            FN G PGIG++R N GDYF GPGLEELFEQLSA   RRGPPPA RS+IDA+P +KI QR
Sbjct: 123 LFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQR 182

Query: 182 HIR-SNSHCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELP-AQGSG 241
           H+R S+S+CPVC+D+FELGSEA+QMPC+H+YHSDCIVPWLVQHNSCPVCRQELP A G  
Sbjct: 183 HLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPS 242

Query: 242 SSHSSS------GSNNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGS-- 301
           SS + +       S++SS +S  RENG     RRNPF+S WPFR+S S+S+ +   G   
Sbjct: 243 SSQNRTTPTRNYRSSSSSSSSNSRENGNE---RRNPFSSFWPFRSSGSSSSSTQNRGGTR 302

Query: 302 NSPALHETN-----QHE---TGYYGWPFD 306
           NS    E +     QH+    GY GWPFD
Sbjct: 303 NSDTSDENHNYHQQQHQQSYMGYSGWPFD 327

[Close]

BLAST of CSPI03G38080.1 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 298.5 bits (763), Expect = 6.1e-81
Identity = 168/332 (50.60%), Postives = 212/332 (63.86%), Query Frame = 0


Query: 2   STANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRLG 61
           S  NTHWC+RC++ V LR RD  C  C GGFV+E+D    R+  D+        ++    
Sbjct: 3   SIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSRAHRDV-------ERDPTFD 62

Query: 62  LLELFSNFMRQRLVDRN---DIRGRLDSIPDHG-PGFGPWLIFGGQIPVRLSG--HGGFE 121
           L+E FS FMR RL +R+   +I GRL S          P LIFGGQ P RL+G  +   E
Sbjct: 63  LMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVE 122

Query: 122 AFFNG-VPGIGVSR-SNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKIT 181
           AF NG  PGIG++R +N GDYF GPGLEEL EQLS+    RGPPPA +SSIDA+P +KIT
Sbjct: 123 AFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKIT 182

Query: 182 QRHIR-SNSHCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS 241
           Q+H++ S+SHCPVC+D+FEL SEA+QMPC H+YHSDCIVPWLVQHNSCPVCR+ELP++GS
Sbjct: 183 QKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGS 242

Query: 242 GSSHSSSGSNNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPAL-- 301
            SS  SS     +R++  REN R    RRN F++LWPFR+SSS+S  +    +N+     
Sbjct: 243 SSSTQSS----QNRSTNGRENSR----RRNIFSNLWPFRSSSSSSTQNRRDTNNTATAEE 302

Query: 302 ----------------HETNQHETGYYGWPFD 306
                           H+  Q   GY GWPFD
Sbjct: 303 GHYHHHQQQQQQHQHQHQQQQSHMGYSGWPFD 319

[Close]

BLAST of CSPI03G38080.1 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 298.5 bits (763), Expect = 6.1e-81
Identity = 168/332 (50.60%), Postives = 212/332 (63.86%), Query Frame = 0


Query: 2   STANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRLG 61
           S  NTHWC+RC++ V LR RD  C  C GGFV+E+D    R+  D+        ++    
Sbjct: 3   SIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSRAHRDV-------ERDPTFD 62

Query: 62  LLELFSNFMRQRLVDRN---DIRGRLDSIPDHG-PGFGPWLIFGGQIPVRLSG--HGGFE 121
           L+E FS FMR RL +R+   +I GRL S          P LIFGGQ P RL+G  +   E
Sbjct: 63  LMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVE 122

Query: 122 AFFNG-VPGIGVSR-SNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKIT 181
           AF NG  PGIG++R +N GDYF GPGLEEL EQLS+    RGPPPA +SSIDA+P +KIT
Sbjct: 123 AFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKIT 182

Query: 182 QRHIR-SNSHCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS 241
           Q+H++ S+SHCPVC+D+FEL SEA+QMPC H+YHSDCIVPWLVQHNSCPVCR+ELP++GS
Sbjct: 183 QKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGS 242

Query: 242 GSSHSSSGSNNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATPGSNSPAL-- 301
            SS  SS     +R++  REN R    RRN F++LWPFR+SSS+S  +    +N+     
Sbjct: 243 SSSTQSS----QNRSTNGRENSR----RRNIFSNLWPFRSSSSSSTQNRRDTNNTATAEE 302

Query: 302 ----------------HETNQHETGYYGWPFD 306
                           H+  Q   GY GWPFD
Sbjct: 303 GHYHHHQQQQQQHQHQHQQQQSHMGYSGWPFD 319

The following BLAST results are available for this feature:

BLAST of CSPI03G38080.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22197	7.0e-90	55.62	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q94AK4	8.6e-80	50.60	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]
Q8LPN7	2.0e-36	32.89	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	8.7e-32	44.51	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q940T5	6.9e-29	32.69	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]

BLAST of CSPI03G38080.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCH6	2.2e-179	99.67	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G822250 PE...	[more]
A0A5A7UFI3	7.8e-177	98.03	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B774	7.8e-177	98.03	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486912 PE=4 ...	[more]
A0A6J1IB00	5.8e-164	90.16	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111473444 P...	[more]
A0A6J1FK80	7.6e-164	90.16	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446055...	[more]

BLAST of CSPI03G38080.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136656.1	4.5e-179	99.67	probable E3 ubiquitin-protein ligase RHC1A [Cucumis sativus] >KGN59473.1 hypothe...	[more]
XP_008443292.1	1.6e-176	98.03	PREDICTED: E3 ubiquitin-protein ligase RING1-like [Cucumis melo] >KAA0053858.1 E...	[more]
XP_038904133.1	3.9e-170	94.10	probable E3 ubiquitin-protein ligase RHC1A isoform X1 [Benincasa hispida]	[more]
XP_038904134.1	3.9e-170	94.10	probable E3 ubiquitin-protein ligase RHC1A isoform X2 [Benincasa hispida]	[more]
KAG7028452.1	1.4e-164	90.82	putative E3 ubiquitin-protein ligase RHC1A, partial [Cucurbita argyrosperma subs...	[more]

BLAST of CSPI03G38080.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G40830.2	5.0e-91	55.62	RING-H2 finger C1A	[more]
AT2G40830.1	5.0e-91	55.62	RING-H2 finger C1A	[more]
AT2G40830.3	5.0e-91	55.62	RING-H2 finger C1A	[more]
AT3G56580.2	6.1e-81	50.60	RING/U-box superfamily protein	[more]
AT3G56580.1	6.1e-81	50.60	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 182..222 e-value: 7.1E-7 score: 38.8
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 181..223 e-value: 1.5E-11 score: 44.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 182..223 score: 12.120453
IPR039525	E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon	PFAM	PF14369	zinc_ribbon_9	coord: 5..36 e-value: 1.8E-9 score: 37.7
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 144..229 e-value: 3.1E-22 score: 80.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 257..293
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 227..246
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 227..305
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 2..294
None	No IPR available	PANTHER	PTHR15710:SF83	E3 UBIQUITIN-PROTEIN LIGASE RHC1A-RELATED	coord: 2..294
None	No IPR available	CDD	cd16667	RING-H2_RNF126_like	coord: 182..223 e-value: 9.13871E-24 score: 89.2992
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 162..229

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G38080	CSPI03G38080	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G38080.1.utr5p1	CSPI03G38080.1.utr5p1	five_prime_UTR
CSPI03G38080.1.utr5p2	CSPI03G38080.1.utr5p2	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G38080.1.cds2	CSPI03G38080.1.cds2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G38080.1.utr3p1	CSPI03G38080.1.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G38080.1	CSPI03G38080.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0061630	ubiquitin protein ligase activity