CSPI03G35570.3 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI03G35570.3
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Nudix hydrolase 26
Location	Chr3: 31002953 .. 31006569 (+)
Sequence length	2593
RNA-Seq Expression	CSPI03G35570.3
Synteny	CSPI03G35570.3

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCAATATTGAAAAAGTAGTATTGTTCCAGTCCAAGGTCGTAGAAACTGCACAGAACGTGATCCTTTTTTCCTTACGTTTCTCACCAAAACAAAAAAAGAAGAAGAAGAAAAGAAACTCTTTCCTCCTATTGGTCAAAATAGTCTCACAAATTTAAAAGAAAAAAAAAAAGAAAATTAGGTGGATGATGGACCCACCTACTTGGGTATGATCAACGGCTGCTGCGATCCTACGGATCAAAATGTAGCCAATGGGAAGGACTTCGGTACACTCTTCTTACGATGTACTCGACTTCACGGGAACAGTCCCCGCACCATTCCTTCTCCTTCTCAAACCTACTCTGTTATTCACACGCACCGCACAATGCACATAACATCAATTCTAAAACAACCTCAATTCGGGTCGGATTATTACTCCGATTCTTTTACGGGTCGGGCTATTGGACCCGCGTGTGACCGGAATCCCGCCGTCACCGCGTCGTTCGCGCAGCAGTGAAATCCCGCCGCCACCCCTCTTTCTTTTGCACTCAAGCAATCTCCGCAGATTCTCCTTTCCGATCTCCCACTCTTGTTCTGCCCCCCATCTCCGCCATCCCCCATCCTCAGGTTGCTCATCTGTATGCTCATTAAATACTTCTCCTTCTCCAATTGCAGCTTCCTAATTCGGTCTTCGTATTCGTATCGATCCATCACCGGCGAGATGAAGCCAGCGAGCAATTCGTTTTCGATGCCTCCGTTCCGTCTCAACAGCATCACATTCTCTGGTGGATTCTTCTTCTTTAATAGATTCGTCTTTGTTTGCGTCTTCGTCGCCGACGAGAAGTAATAATCCTCTTCGTTGTCGTGGATGACCTTTCGCCTCCTCTCGCGTCTTGACCGTTTCAAAACCACAAGAAAGAAACTATTCTTCTCGCCGGAGATTGCGCCAAAATTGACCTTCCCAGTAATAGGAATTCTCCGCCGAATCTTCGTGGAGGAATCCAGAGAGATCTTGTACTCCGCGAGATAAACCGTGAACTGGTGCGAAGAGATTCCAATCATCTTGCTCAAACTGGACTGGAAATTCTTGAACTCCGTCGAAGAAGAAACGATAACGGAACCGATGTTGGTATCACGTTCGCCATCGTGGAAAACGATCGGGAACGAAACGCCCTCGACGGTCTCCGCCATCGCCTCTACAAATAAAAGAAAAACTCTTCAATTAAGGAACAGAAGGAAACTTTCATAAAAAAAGAAAGAAAGAAAGAAGAGGGTTTTACGGAGAAATCTTGAAGAAGACTATGGAAGGTTTTTACTTTAACAAGTGGGTTTTAGGTTTTTATGGTAACAACCTCAAGGCGGTGAGGGTATTTTCGGAATAAGATATTAGATTTATGGCCTTCGTACCTAAGGAAGCCAAACGAAGAACTAAGAAGGAAACTTCAAAACGTTTATATAGAAGGAAAGAGACTTGGGAATCAAGTAAAATATAAACGACATCGTAATAGTCAGCTTGGCTGTGTTTAGACCAAAATGCCCCCGTCTTCTAATTAAATCCTCGTCAACGCATTTTTGGAAGGGGTCGTCTTCTTCTTCATCTACCATCCTCCATCCATGGAGTTTCCTCCAGAAGGTTACAGAAGAGGCGTTGGGATTTGCCTTCTTAATTCTTCTGGAAAAGTAATTTCGCTTCGTTGCCTTCTTCTTTCGCTCTTATCTTGTTTTCTTCTGATCATAACCAATTTTTCTCTCTCCAATTCATCTTTTGCAGATTTTTGCAGCTTCGAGACTGAACGGGCATGAAATCTGGGAAATGCCTCAGGTAGTTTCTATTCCGTTGTATTTTCAGCTTTATTCATTGGGAAACAGGAAAAGTACTATCTCTCTGCTTTTCTTTAAGTACTTCTCTCGCTCTCGCTTTATTCTGTGCGAATTAGGGCACATATTTATAGGTTCCATCTCTGATTTCCTTGTGGGAATATCCCAAAATTCTTTCTTGAGATCCCTTTTTGAAGAAATTCGAGGATCAGCTGAAAATGAGAATGGGATTTTTGAGTAATCTGTACCCATTTCTCTCGAATTTTCTCTTTGAACGTAGTGTTGGTGGAATTCTTGCAATGGTGCTGCGGGTTCTGGTTCTTTCCATTAGAGAATTGTATTAGCTTTCAGAAAGCTGTTTATTATGGTTCTGATGGAAACTTTGGTGGGATTCTCATCTTTTCAATCATGAGACGGAGAGGTGTTTGATATTATGTCTTTATTTCTTTTACAATTGGAGTATCTACATGAGTTAGCTTATATACTATTTAACTAGGAAACTCATAGTTGACTTCAAATGATGGATTGATCCAGTAACTTCAAAATCATATAGATCTGTATTGCCTTGTGGAACTTGAAGCTTGTGGAGGATCCTTAAGTTTGTGTTCCCTGACACTGATTTTCCATCATACAGGGTGGTGTAAATGAAGGGGAAGATCTCAAAACTGCAGCCAAGAGAGAACTCATGGAAGAAACAGGAGTAGTTTCTGCAGAAATTATAGCTGAGGTATGCATTTTGAGATGGTTACAGTTATATAACTTTGCAGCTTCCATATCTCGTCTTAAGTACACTGTATGATGAACTTTAGAAAAATGAATTCTGGCTTCATTTGAATTGGGACTGATTCGATCCATGGTTAAATATAGCTACCCTACTGGCTTTCTTACGATTTCTCTCCCGAAGTAAGCATCAGACACATCAAACACTGGGGAGTCAGATACAAAGGCCAGACCCATAAGTGGTATTGCCTCCATAAAATCCGACTCCTATAACATTTTCTTGTTAAGGAACTTTCCTTTTTGGAAAGCATCTCCACATTTTTTTTTTTACTTTCTTTGCATGATCCTATTTTCTTTTAATCCTCAAAGAACTGTACATAGTTGGTAACTTTTTCTTTCTTTCAGCTTCAAAGAATTGATCAACGTATATGGATTATATTATTTCCCCTTCAAGCTTCCGCGTTCAATTGATCAATCATAAAGCTCGTGAACAGGTTTCTCATGAAGTTTACCGGAAAAGACGAAGAGATAAATCTTCTAGGTGATGGAACAGAAAGGCAAGAGTTTGGGAAATGGTCATGGATGTCAATTGAACAGCTACTGAATCAAGTGTGTAAACAACTTCTACCTAATTCATTCTTTCTCCTGCAACAATTTTTCATAAACCTTTACAATCATTTGAACAGGCTAAGAAATCCCGGAAGCCTGTTTACGAACAAGTGATGAAGGAGTTTGGTAACATCATACTTGGAATTGTGGGTGACGATCTTTCTCTCACGCAGAGTGAAACTCAATTGGTTATTTGATGGGTTTCGAATATATTTATCGATAGTACCACGAACATATACCATACGAATCACAGGTTCATATAAGATGTTATGTACTCATATCTAAGTTTGATTTTTAAAATAAGAGATGCCTTTATTTAGAGATAAGGCAGATATGTTTGATCCTCTTGGAATTTGAAGTGGTGGATTAATGAAATCTGAGGCCAAGATATTGGAATCAGAACCTTCACTAAATTGTGCAGCTGTAAAAGGATTTAAGTGTCCAAATAAGAGATAAAGGGATTTTGATTTATAGAAAGGAATCAAAACGTGGG

mRNA sequence

ATCAATATTGAAAAAGTAGTATTGTTCCAGTCCAAGGTCGTAGAAACTGCACAGAACGTGATCCTTTTTTCCTTACGTTTCTCACCAAAACAAAAAAAGAAGAAGAAGAAAAGAAACTCTTTCCTCCTATTGGTCAAAATAGTCTCACAAATTTAAAAGAAAAAAAAAAAGAAAATTAGGTGGATGATGGACCCACCTACTTGGGTATGATCAACGGCTGCTGCGATCCTACGGATCAAAATGTAGCCAATGGGAAGGACTTCGGTACACTCTTCTTACGATGTACTCGACTTCACGGGAACAGTCCCCGCACCATTCCTTCTCCTTCTCAAACCTACTCTGTTATTCACACGCACCGCACAATGCACATAACATCAATTCTAAAACAACCTCAATTCGGGTCGGATTATTACTCCGATTCTTTTACGGGTCGGGCTATTGGACCCGCGTGTGACCGGAATCCCGCCGTCACCGCGTCGTTCGCGCAGCAGTGAAATCCCGCCGCCACCCCTCTTTCTTTTGCACTCAAGCAATCTCCGCAGATTCTCCTTTCCGATCTCCCACTCTTGTTCTGCCCCCCATCTCCGCCATCCCCCATCCTCAGGTTGCTCATCTGTATGCTCATTAAATACTTCTCCTTCTCCAATTGCAGCTTCCTAATTCGGTCTTCGTATTCGTATCGATCCATCACCGGCGAGATGAAGCCAGCGAGCAATTCGTTTTCGATGCCTCCGTTCCGTCTCAACAGCATCACATTCTCTGGTGGATTCTTCTTCTTTAATAGATTCGTCTTTGTTTGCGTCTTCGTCGCCGACGAGAAGTAATAATCCTCTTCGTTGTCGTGGATGACCTTTCGCCTCCTCTCGCGTCTTGACCGTTTCAAAACCACAAGAAAGAAACTATTCTTCTCGCCGGAGATTGCGCCAAAATTGACCTTCCCAGTAATAGGAATTCTCCGCCGAATCTTCGTGGAGGAATCCAGAGAGATCTTGTACTCCGCGAGATAAACCGTGAACTGGTGCGAAGAGATTCCAATCATCTTGCTCAAACTGGACTGGAAATTCTTGAACTCCGTCGAAGAAGAAACGATAACGGAACCGATGTTGGTATCACGTTCGCCATCGTGGAAAACGATCGGGAACGAAACGCCCTCGACGGTCTCCGCCATCGCCTCTACAAATAAAAGAAAAACTCTTCAATTAAGGAACAGAAGGAAACTTTCATAAAAAAAGAAAGAAAGAAAGAAGAGGGTTTTACGGAGAAATCTTGAAGAAGACTATGGAAGGTTTTTACTTTAACAAGTGGGTTTTAGGTTTTTATGGTAACAACCTCAAGGCGGTGAGGGTATTTTCGGAATAAGATATTAGATTTATGGCCTTCGTACCTAAGGAAGCCAAACGAAGAACTAAGAAGGAAACTTCAAAACGTTTATATAGAAGGAAAGAGACTTGGGAATCAAGTAAAATATAAACGACATCGTAATAGTCAGCTTGGCTGTGTTTAGACCAAAATGCCCCCGTCTTCTAATTAAATCCTCGTCAACGCATTTTTGGAAGGGGTCGTCTTCTTCTTCATCTACCATCCTCCATCCATGGAGTTTCCTCCAGAAGGTTACAGAAGAGGCGTTGGGATTTGCCTTCTTAATTCTTCTGGAAAAATTTTTGCAGCTTCGAGACTGAACGGGCATGAAATCTGGGAAATGCCTCAGGGTGGTGTAAATGAAGGGGAAGATCTCAAAACTGCAGCCAAGAGAGAACTCATGGAAGAAACAGGAGTAGTTTCTGCAGAAATTATAGCTGAGCTACCCTACTGGCTTTCTTACGATTTCTCTCCCGAAGTAAGCATCAGACACATCAAACACTGGGGAGTCAGATACAAAGGCCAGACCCATAAGTGCTTCAAAGAATTGATCAACGTATATGGATTATATTATTTCCCCTTCAAGCTTCCGCGTTCAATTGATCAATCATAAAGCTCGTGAACAGGTTTCTCATGAAGTTTACCGGAAAAGACGAAGAGATAAATCTTCTAGGTGATGGAACAGAAAGGCAAGAGTTTGGGAAATGGTCATGGATGTCAATTGAACAGCTACTGAATCAAGTGTGTAAACAACTTCTACCTAATTCATTCTTTCTCCTGCAACAATTTTTCATAAACCTTTACAATCATTTGAACAGGCTAAGAAATCCCGGAAGCCTGTTTACGAACAAGTGATGAAGGAGTTTGGTAACATCATACTTGGAATTGTGGGTGACGATCTTTCTCTCACGCAGAGTGAAACTCAATTGGTTATTTGATGGGTTTCGAATATATTTATCGATAGTACCACGAACATATACCATACGAATCACAGGTTCATATAAGATGTTATGTACTCATATCTAAGTTTGATTTTTAAAATAAGAGATGCCTTTATTTAGAGATAAGGCAGATATGTTTGATCCTCTTGGAATTTGAAGTGGTGGATTAATGAAATCTGAGGCCAAGATATTGGAATCAGAACCTTCACTAAATTGTGCAGCTGTAAAAGGATTTAAGTGTCCAAATAAGAGATAAAGGGATTTTGATTTATAGAAAGGAATCAAAACGTGGG

Coding sequence (CDS)

ATGGAGTTTCCTCCAGAAGGTTACAGAAGAGGCGTTGGGATTTGCCTTCTTAATTCTTCTGGAAAAATTTTTGCAGCTTCGAGACTGAACGGGCATGAAATCTGGGAAATGCCTCAGGGTGGTGTAAATGAAGGGGAAGATCTCAAAACTGCAGCCAAGAGAGAACTCATGGAAGAAACAGGAGTAGTTTCTGCAGAAATTATAGCTGAGCTACCCTACTGGCTTTCTTACGATTTCTCTCCCGAAGTAAGCATCAGACACATCAAACACTGGGGAGTCAGATACAAAGGCCAGACCCATAAGTGCTTCAAAGAATTGATCAACGTATATGGATTATATTATTTCCCCTTCAAGCTTCCGCGTTCAATTGATCAATCATAA

Protein sequence

MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEETGVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCFKELINVYGLYYFPFKLPRSIDQS*

Homology

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BLAST of CSPI03G35570.3 vs. ExPASy Swiss-Prot
Match: Q9CAF2 (Nudix hydrolase 26, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT26 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 6.8e-31
Identity = 62/103 (60.19%), Postives = 76/103 (73.79%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           ME PPEGYRR VG+CL+NSS KIF ASRL+    W+MPQGG++EGED + A  REL EET
Sbjct: 56  MESPPEGYRRNVGVCLMNSSKKIFTASRLDIPSAWQMPQGGIDEGEDPRVAVMRELKEET 115

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCF 104
           GV SAEI+AE P+W++YDF P+V  +    WG  +KGQ  K F
Sbjct: 116 GVHSAEILAEAPHWITYDFPPDVREKLKVRWGSDWKGQAQKWF 158

[Close]

BLAST of CSPI03G35570.3 vs. ExPASy Swiss-Prot
Match: Q9FNH4 (Nudix hydrolase 27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT27 PE=1 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 3.2e-28
Identity = 62/118 (52.54%), Postives = 79/118 (66.95%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           +E PP GYR+ VGICL++   KIF AS+++  + W+MPQGG +EGEDL+ AA REL EET
Sbjct: 55  VESPPVGYRKNVGICLVSPCRKIFTASKIHIPDTWQMPQGGADEGEDLRNAAFRELREET 114

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCF-------KELINVYG 112
           GV SAE IAE+P WL+YDF  EV  +  + W   YKGQ  K F       +E IN+ G
Sbjct: 115 GVTSAEFIAEIPNWLTYDFPREVKDKLNRKWRTSYKGQAQKWFLFKFTGKEEEINLLG 172

[Close]

BLAST of CSPI03G35570.3 vs. ExPASy Swiss-Prot
Match: Q9C6Z2 (Nudix hydrolase 25 OS=Arabidopsis thaliana OX=3702 GN=NUDT25 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 9.2e-28
Identity = 57/103 (55.34%), Postives = 72/103 (69.90%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           ME  P GYR  VG+CL+NS   +F ASRLN    W+MPQGG+ +GED K+AA REL EET
Sbjct: 1   MENLPPGYRPNVGVCLINSDNLVFVASRLNVPGAWQMPQGGIEDGEDPKSAAMRELQEET 60

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCF 104
           GVVSAEI++E+P WL+YDF P V  +  + WG  + GQ  K +
Sbjct: 61  GVVSAEIVSEVPNWLTYDFPPAVKAKVNRLWGGEWHGQAQKWY 103

[Close]

BLAST of CSPI03G35570.3 vs. ExPASy Swiss-Prot
Match: Q2RV14 (RNA pyrophosphohydrolase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIMB 8255 / S1) OX=269796 GN=rppH PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 7.8e-19
Identity = 46/96 (47.92%), Postives = 63/96 (65.62%), Query Frame = 0


Query: 8   YRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEETGVVSAEI 67
           YR+GVGI L+N+ G++F A RL+  E W+MPQGG++ GED +TAA RE+ EE G  +A +
Sbjct: 14  YRQGVGIMLINARGQVFVARRLDSPEAWQMPQGGIDAGEDPETAAWREMEEEIGTRNALL 73

Query: 68  IAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCF 104
           + E   WL YD   E+  R    WG R++GQ  K F
Sbjct: 74  LGETAGWLGYDLPEELRGR---LWGGRFQGQRQKWF 106

[Close]

BLAST of CSPI03G35570.3 vs. ExPASy Swiss-Prot
Match: A8LKJ8 (RNA pyrophosphohydrolase OS=Dinoroseobacter shibae (strain DSM 16493 / NCIMB 14021 / DFL 12) OX=398580 GN=rppH PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.5e-17
Identity = 44/97 (45.36%), Postives = 61/97 (62.89%), Query Frame = 0


Query: 8   YRRGVGICLLNSSGKIFAASRLNGH-EIWEMPQGGVNEGEDLKTAAKRELMEETGVV--S 67
           YR+ VG+ L + +G++F   R +     W+MPQGG+ +GED +TAA REL+EETGV    
Sbjct: 11  YRKCVGVVLWDGAGRVFTGQRYDSELPAWQMPQGGIEDGEDARTAALRELVEETGVAVEK 70

Query: 68  AEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHK 102
            E++AE P W+ YD  PE+  R    W  RYKGQ  +
Sbjct: 71  VEVLAETPDWIKYDLPPEIVPR---IWKGRYKGQAQR 104

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BLAST of CSPI03G35570.3 vs. ExPASy TrEMBL
Match: A0A0A0LBJ8 (Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G781040 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.9e-68
Identity = 125/126 (99.21%), Postives = 125/126 (99.21%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           MEFPPEGYRRGVGICLLNSSGKIFAASR NGHEIWEMPQGGVNEGEDLKTAAKRELMEET
Sbjct: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRPNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCFKELINVYGLYYFPFKLP 120
           GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCFKELINVYGLYYFPFKLP
Sbjct: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCFKELINVYGLYYFPFKLP 120

Query: 121 RSIDQS 127
           RSIDQS
Sbjct: 121 RSIDQS 126

[Close]

BLAST of CSPI03G35570.3 vs. ExPASy TrEMBL
Match: A0A5D3DP41 (Nudix hydrolase 26 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004530 PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.5e-52
Identity = 100/103 (97.09%), Postives = 100/103 (97.09%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           MEFPPEGYRRGVGICL NSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET
Sbjct: 1   MEFPPEGYRRGVGICLFNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCF 104
           GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWG RYKGQTHK F
Sbjct: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGARYKGQTHKWF 103

[Close]

BLAST of CSPI03G35570.3 vs. ExPASy TrEMBL
Match: A0A1S3B8G6 (nudix hydrolase 26, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103487152 PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 2.5e-52
Identity = 100/103 (97.09%), Postives = 100/103 (97.09%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           MEFPPEGYRRGVGICL NSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET
Sbjct: 1   MEFPPEGYRRGVGICLFNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCF 104
           GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWG RYKGQTHK F
Sbjct: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGARYKGQTHKWF 103

[Close]

BLAST of CSPI03G35570.3 vs. ExPASy TrEMBL
Match: A0A5A7TPT1 (Nudix hydrolase 26 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G001500 PE=3 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 1.0e-50
Identity = 100/106 (94.34%), Postives = 100/106 (94.34%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           MEFPPEGYRRGVGICL NSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET
Sbjct: 1   MEFPPEGYRRGVGICLFNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIR---HIKHWGVRYKGQTHKCF 104
           GVVSAEIIAELPYWLSYDFSPEVSIR   HIKHWG RYKGQTHK F
Sbjct: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHIKHWGARYKGQTHKWF 106

[Close]

BLAST of CSPI03G35570.3 vs. ExPASy TrEMBL
Match: A0A6J1J4S6 (nudix hydrolase 26, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111481747 PE=3 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 5.6e-44
Identity = 91/118 (77.12%), Postives = 97/118 (82.20%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           MEFPPEGYRR VGICL N SGKIFAASRLN  E+WEMPQGGVNEGEDL TAAKREL EET
Sbjct: 44  MEFPPEGYRRNVGICLFNPSGKIFAASRLNRPEVWEMPQGGVNEGEDLNTAAKRELTEET 103

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCF-------KELINVYG 112
           GVVSAEII+ELPYWL+YDFSPE SI+  KHWGVRYKGQT K F       +E IN+ G
Sbjct: 104 GVVSAEIISELPYWLTYDFSPETSIKLSKHWGVRYKGQTQKWFLMKFTGKEEEINLLG 161

[Close]

BLAST of CSPI03G35570.3 vs. NCBI nr
Match: KGN59203.1 (hypothetical protein Csa_001538 [Cucumis sativus])

HSP 1 Score: 268.1 bits (684), Expect = 3.9e-68
Identity = 125/126 (99.21%), Postives = 125/126 (99.21%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           MEFPPEGYRRGVGICLLNSSGKIFAASR NGHEIWEMPQGGVNEGEDLKTAAKRELMEET
Sbjct: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRPNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCFKELINVYGLYYFPFKLP 120
           GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCFKELINVYGLYYFPFKLP
Sbjct: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCFKELINVYGLYYFPFKLP 120

Query: 121 RSIDQS 127
           RSIDQS
Sbjct: 121 RSIDQS 126

[Close]

BLAST of CSPI03G35570.3 vs. NCBI nr
Match: XP_031739122.1 (nudix hydrolase 26, chloroplastic [Cucumis sativus])

HSP 1 Score: 214.9 bits (546), Expect = 3.9e-52
Identity = 101/103 (98.06%), Postives = 101/103 (98.06%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           MEFPPEGYRRGVGICLLNSSGKIFAASR NGHEIWEMPQGGVNEGEDLKTAAKRELMEET
Sbjct: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRPNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCF 104
           GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHK F
Sbjct: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKWF 103

[Close]

BLAST of CSPI03G35570.3 vs. NCBI nr
Match: XP_008443595.2 (PREDICTED: nudix hydrolase 26, chloroplastic-like [Cucumis melo] >TYK25315.1 nudix hydrolase 26 [Cucumis melo var. makuwa])

HSP 1 Score: 214.5 bits (545), Expect = 5.2e-52
Identity = 100/103 (97.09%), Postives = 100/103 (97.09%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           MEFPPEGYRRGVGICL NSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET
Sbjct: 1   MEFPPEGYRRGVGICLFNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCF 104
           GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWG RYKGQTHK F
Sbjct: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGARYKGQTHKWF 103

[Close]

BLAST of CSPI03G35570.3 vs. NCBI nr
Match: KAA0043817.1 (nudix hydrolase 26 [Cucumis melo var. makuwa])

HSP 1 Score: 209.1 bits (531), Expect = 2.2e-50
Identity = 100/106 (94.34%), Postives = 100/106 (94.34%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           MEFPPEGYRRGVGICL NSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET
Sbjct: 1   MEFPPEGYRRGVGICLFNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIR---HIKHWGVRYKGQTHKCF 104
           GVVSAEIIAELPYWLSYDFSPEVSIR   HIKHWG RYKGQTHK F
Sbjct: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHIKHWGARYKGQTHKWF 106

[Close]

BLAST of CSPI03G35570.3 vs. NCBI nr
Match: XP_038904841.1 (nudix hydrolase 26, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 205.7 bits (522), Expect = 2.4e-49
Identity = 95/103 (92.23%), Postives = 99/103 (96.12%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           MEFPPEGYRRGVGICL NSSGKIFAASRLN  EIWEMPQGG+NEGEDLKTAAKREL+EET
Sbjct: 1   MEFPPEGYRRGVGICLFNSSGKIFAASRLNRPEIWEMPQGGINEGEDLKTAAKRELIEET 60

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCF 104
           G+VSAEIIAELPYWLSYDFSPEVSI+HIKHWGVRYKGQTHK F
Sbjct: 61  GIVSAEIIAELPYWLSYDFSPEVSIKHIKHWGVRYKGQTHKWF 103

[Close]

BLAST of CSPI03G35570.3 vs. TAIR 10
Match: AT3G10620.1 (nudix hydrolase homolog 26 )

HSP 1 Score: 134.8 bits (338), Expect = 4.8e-32
Identity = 62/103 (60.19%), Postives = 76/103 (73.79%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           ME PPEGYRR VG+CL+NSS KIF ASRL+    W+MPQGG++EGED + A  REL EET
Sbjct: 56  MESPPEGYRRNVGVCLMNSSKKIFTASRLDIPSAWQMPQGGIDEGEDPRVAVMRELKEET 115

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCF 104
           GV SAEI+AE P+W++YDF P+V  +    WG  +KGQ  K F
Sbjct: 116 GVHSAEILAEAPHWITYDFPPDVREKLKVRWGSDWKGQAQKWF 158

[Close]

BLAST of CSPI03G35570.3 vs. TAIR 10
Match: AT5G06340.1 (nudix hydrolase homolog 27 )

HSP 1 Score: 125.9 bits (315), Expect = 2.2e-29
Identity = 62/118 (52.54%), Postives = 79/118 (66.95%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           +E PP GYR+ VGICL++   KIF AS+++  + W+MPQGG +EGEDL+ AA REL EET
Sbjct: 55  VESPPVGYRKNVGICLVSPCRKIFTASKIHIPDTWQMPQGGADEGEDLRNAAFRELREET 114

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCF-------KELINVYG 112
           GV SAE IAE+P WL+YDF  EV  +  + W   YKGQ  K F       +E IN+ G
Sbjct: 115 GVTSAEFIAEIPNWLTYDFPREVKDKLNRKWRTSYKGQAQKWFLFKFTGKEEEINLLG 172

[Close]

BLAST of CSPI03G35570.3 vs. TAIR 10
Match: AT1G30110.1 (nudix hydrolase homolog 25 )

HSP 1 Score: 124.4 bits (311), Expect = 6.5e-29
Identity = 57/103 (55.34%), Postives = 72/103 (69.90%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           ME  P GYR  VG+CL+NS   +F ASRLN    W+MPQGG+ +GED K+AA REL EET
Sbjct: 1   MENLPPGYRPNVGVCLINSDNLVFVASRLNVPGAWQMPQGGIEDGEDPKSAAMRELQEET 60

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCF 104
           GVVSAEI++E+P WL+YDF P V  +  + WG  + GQ  K +
Sbjct: 61  GVVSAEIVSEVPNWLTYDFPPAVKAKVNRLWGGEWHGQAQKWY 103

[Close]

BLAST of CSPI03G35570.3 vs. TAIR 10
Match: AT1G30110.2 (nudix hydrolase homolog 25 )

HSP 1 Score: 124.4 bits (311), Expect = 6.5e-29
Identity = 57/103 (55.34%), Postives = 72/103 (69.90%), Query Frame = 0


Query: 1   MEFPPEGYRRGVGICLLNSSGKIFAASRLNGHEIWEMPQGGVNEGEDLKTAAKRELMEET 60
           ME  P GYR  VG+CL+NS   +F ASRLN    W+MPQGG+ +GED K+AA REL EET
Sbjct: 1   MENLPPGYRPNVGVCLINSDNLVFVASRLNVPGAWQMPQGGIEDGEDPKSAAMRELQEET 60

Query: 61  GVVSAEIIAELPYWLSYDFSPEVSIRHIKHWGVRYKGQTHKCF 104
           GVVSAEI++E+P WL+YDF P V  +  + WG  + GQ  K +
Sbjct: 61  GVVSAEIVSEVPNWLTYDFPPAVKAKVNRLWGGEWHGQAQKWY 103

[Close]

BLAST of CSPI03G35570.3 vs. TAIR 10
Match: AT2G04450.1 (nudix hydrolase homolog 6 )

HSP 1 Score: 45.1 bits (105), Expect = 5.0e-05
Identity = 23/60 (38.33%), Postives = 35/60 (58.33%), Query Frame = 0


Query: 8   YRRGVGICLLNSSGK-IFAASRLNGH----EIWEMPQGGVNEGEDLKTAAKRELMEETGV 63
           +R GVG  +LN   K +     ++GH     +W++P G V EGE++   A RE+ EETG+
Sbjct: 102 HRIGVGAFVLNKKTKEVLVVQEIDGHFKGTGVWKLPTGVVKEGENIWEGALREVEEETGI 161

The following BLAST results are available for this feature:

BLAST of CSPI03G35570.3 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9CAF2	6.8e-31	60.19	Nudix hydrolase 26, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT26 PE=1...	[more]
Q9FNH4	3.2e-28	52.54	Nudix hydrolase 27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT27 PE=1...	[more]
Q9C6Z2	9.2e-28	55.34	Nudix hydrolase 25 OS=Arabidopsis thaliana OX=3702 GN=NUDT25 PE=1 SV=1	[more]
Q2RV14	7.8e-19	47.92	RNA pyrophosphohydrolase OS=Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1...	[more]
A8LKJ8	1.5e-17	45.36	RNA pyrophosphohydrolase OS=Dinoroseobacter shibae (strain DSM 16493 / NCIMB 140...	[more]

BLAST of CSPI03G35570.3 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LBJ8	1.9e-68	99.21	Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G78...	[more]
A0A5D3DP41	2.5e-52	97.09	Nudix hydrolase 26 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G0...	[more]
A0A1S3B8G6	2.5e-52	97.09	nudix hydrolase 26, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103487152 P...	[more]
A0A5A7TPT1	1.0e-50	94.34	Nudix hydrolase 26 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G0...	[more]
A0A6J1J4S6	5.6e-44	77.12	nudix hydrolase 26, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114817...	[more]

BLAST of CSPI03G35570.3 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN59203.1	3.9e-68	99.21	hypothetical protein Csa_001538 [Cucumis sativus]	[more]
XP_031739122.1	3.9e-52	98.06	nudix hydrolase 26, chloroplastic [Cucumis sativus]	[more]
XP_008443595.2	5.2e-52	97.09	PREDICTED: nudix hydrolase 26, chloroplastic-like [Cucumis melo] >TYK25315.1 nud...	[more]
KAA0043817.1	2.2e-50	94.34	nudix hydrolase 26 [Cucumis melo var. makuwa]	[more]
XP_038904841.1	2.4e-49	92.23	nudix hydrolase 26, chloroplastic-like [Benincasa hispida]	[more]

BLAST of CSPI03G35570.3 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10620.1	4.8e-32	60.19	nudix hydrolase homolog 26	[more]
AT5G06340.1	2.2e-29	52.54	nudix hydrolase homolog 27	[more]
AT1G30110.1	6.5e-29	55.34	nudix hydrolase homolog 25	[more]
AT1G30110.2	6.5e-29	55.34	nudix hydrolase homolog 25	[more]
AT2G04450.1	5.0e-05	38.33	nudix hydrolase homolog 6	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020476	NUDIX hydrolase	PRINTS	PR00502	NUDIXFAMILY	coord: 35..49 score: 38.64 coord: 49..64 score: 47.79
None	No IPR available	GENE3D	3.90.79.10	Nucleoside Triphosphate Pyrophosphohydrolase	coord: 1..109 e-value: 9.9E-29 score: 102.2
None	No IPR available	PANTHER	PTHR11839:SF21	NUDIX HYDROLASE 27, CHLOROPLASTIC-LIKE	coord: 1..103
None	No IPR available	PANTHER	PTHR11839	UDP/ADP-SUGAR PYROPHOSPHATASE	coord: 1..103
IPR000086	NUDIX hydrolase domain	PFAM	PF00293	NUDIX	coord: 8..90 e-value: 4.7E-18 score: 65.5
IPR000086	NUDIX hydrolase domain	PROSITE	PS51462	NUDIX	coord: 7..126 score: 12.970398
IPR020084	NUDIX hydrolase, conserved site	PROSITE	PS00893	NUDIX_BOX	coord: 40..61
IPR015797	NUDIX hydrolase-like domain superfamily	SUPERFAMILY	55811	Nudix	coord: 5..102

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G35570	CSPI03G35570	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G35570.3.utr5p1	CSPI03G35570.3.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G35570.3.cds1	CSPI03G35570.3.cds1	CDS
CSPI03G35570.3.cds2	CSPI03G35570.3.cds2	CDS
CSPI03G35570.3.cds3	CSPI03G35570.3.cds3	CDS
CSPI03G35570.3.cds4	CSPI03G35570.3.cds4	CDS
CSPI03G35570.3.cds5	CSPI03G35570.3.cds5	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G35570.3.utr3p1	CSPI03G35570.3.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G35570.3	CSPI03G35570.3-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006753	nucleoside phosphate metabolic process
biological_process	GO:0019693	ribose phosphate metabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0034432	bis(5'-adenosyl)-pentaphosphatase activity
molecular_function	GO:0008893	guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity