CSPI03G33520.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI03G33520.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Protein NUCLEAR FUSION DEFECTIVE 4-like
Location	Chr3: 29571786 .. 29573946 (+)
Sequence length	2161
RNA-Seq Expression	CSPI03G33520.1
Synteny	CSPI03G33520.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTTCTCTCTATGAACAAACTAATCCAATTCCTGATAAGTGGCGGAGAGAGTAGCAGCAGCAAAAGAGTTTAGCTAAAATAAAACACCACACAATATCATACCTTCCATCAAACCTCCTTTCACAATGTATTTATACACTCCCATTCCTTCACCATTTCATCTCCAAATTCTCCTTCCCCAACACAAATGGCAGCCTCATCCTCAAATCCCACCTCCTCTAACCCCTTCTGGTACCAGTTTCTCTTTGGCCGCTGGTTCTCTGTCCTCGCTTCCATTCTCATCATGTCCGTCGCCGGTGCTACCTACATGTTCGCCCTCTACTCCTCCGACATCAAATCTTCCTTAGGCTACGACCAAACCACCCTCAATTTGCTCAGTTTCTTCAAGGACTTGGGCGGCAATGTTGGCGTCATCAGTGGTCTCACCAACGAGGTCGCTCCTGCCTGGGTCGTTCTTCTTATTGGTGCCGTCATGAACCTTTTTGGCTACACCATGATTTGGCTGGCCGTAACTAAAAGAATACCCAAACCCCAAATCTGGCATATGTGTCTCTATATCACCATCGGAGCTAATTCTCAGACCTTTGCAAATACTGGGGCTCTTGTAACCTGCGTCAAAAACTTCCCGGAAAGTCGTGGTAGTGTTTTGGGGCTTTTGAAGGGGTTCGTTGGCTTAAGCGGCGCCATTTTGTCCCAACTTTACCGTGCCTTTTATGGCAATAATCCCGAATCTCTTATCCTCTTAATCGCTTGGCTTCCCGCTGCCGTCTCTGTTGTTTTGCTCCGTTTCGTTCGCATCATTAAGGATCTCCGGCAGCCCAACGAGCTCAAAGTTTTTTACCATTTCCTTTACATTTCTCTCGGCCTTGCTGGGACTCTAATGGTTTTGATTATTTTGCAAAGTCTTCTCCGATTTCAACAAATACAATACGTCGGTAGTGCCATTGTTGTGATTGTTCTGCTTTTACTACCTCTGACCATTGTTTTCAGAGAGGAATTGAGTGTTTGGAAGAGTAAAATTGCAAGCCCAGTTTTACAATTAGAGTCGGCATCTCAACAACCACCGCCGCCACTGACTTCGACGGTGTCTCTTGCGCCATCGAGCCCTCCGTCGGAATCCTGCTTTAAAAACATGTTTAATCCTCCAAGCAGAGGAGAAGACTACACAATCCCACAAGCCATTTTCAGTATGGATTTGATAATTCTTTTTATGGCCACAATTTGTGGTGTCGGTGGAACATTGACGGCGATCGACAATTTGGGCCAAATCGGAGAGTCTTTGGGGTACCAATCTCACAGTATCACCACATTCATCTCTCTCGTCAGCATATGGAACTACCTCGGCCGTGTGGTCTCCGGATTCGTATCGGAATATTTCTGGAAAAAGTACAAAGTCCCACGTCCTCTGTTTCTCTTCGCGACACTTATTCTGTCCTGCGTCGGTCATCTTCTAATAGCATTCGGAGTTCCGAATTCATTGTATTTTTCTTCAATCGTAATCGGGTTCTGCTTCGGCGCACAGTGGCCATTGATCTATGCAATCATATCGGAGATTTTCGGGTTGAAGTACTACGCGACTTTGTCCAGTATGAGTGGAGGGGCGAGTCCGATTGGGGCATATATCTTGAACGTGAGAGTGGCGGGTCATTTGTACGACAGAGAGGCGCAAAGGCAGATGGAGGCAACGGGGCGACGGAGAAACATAGGAGAGGATTTGAGTTGCTTAGGAGTGGAATGTTACCGAAAGGCATTTCTTATAATCACGGCGGCGACGGTATTCGGGGCACTTGTTTCGCTGATTTTGGTGGTTCGGACATGGAAGTTCTATAAGGGCGATATTTACCGGAAGTTCAGAGAGGTGGAAGGTGAAGACATCGAAATGAAAATGGCCGCTCCGACCAACGGTACGTTGACGGCAGCGAAAAACTGAATCTGTTTAGTATATTTCTTAATGAGTATTTTCTCTACCAAATTAGAACTTTACTTATCATTGTACATTTTATCCAATCATCTTCCCATAGTTAGTTTCACAGTGAACTAATGTACACATGCATTAGGAGCCAACTTAAGGAATAAAAATGTACCACAACCAATACATCTTTGACATACTCAACAAATTATCTGATCTATGTGCTTGTTTTGTATATATATATATATATAT

mRNA sequence

CTTTCTCTCTATGAACAAACTAATCCAATTCCTGATAAGTGGCGGAGAGAGTAGCAGCAGCAAAAGAGTTTAGCTAAAATAAAACACCACACAATATCATACCTTCCATCAAACCTCCTTTCACAATGTATTTATACACTCCCATTCCTTCACCATTTCATCTCCAAATTCTCCTTCCCCAACACAAATGGCAGCCTCATCCTCAAATCCCACCTCCTCTAACCCCTTCTGGTACCAGTTTCTCTTTGGCCGCTGGTTCTCTGTCCTCGCTTCCATTCTCATCATGTCCGTCGCCGGTGCTACCTACATGTTCGCCCTCTACTCCTCCGACATCAAATCTTCCTTAGGCTACGACCAAACCACCCTCAATTTGCTCAGTTTCTTCAAGGACTTGGGCGGCAATGTTGGCGTCATCAGTGGTCTCACCAACGAGGTCGCTCCTGCCTGGGTCGTTCTTCTTATTGGTGCCGTCATGAACCTTTTTGGCTACACCATGATTTGGCTGGCCGTAACTAAAAGAATACCCAAACCCCAAATCTGGCATATGTGTCTCTATATCACCATCGGAGCTAATTCTCAGACCTTTGCAAATACTGGGGCTCTTGTAACCTGCGTCAAAAACTTCCCGGAAAGTCGTGGTAGTGTTTTGGGGCTTTTGAAGGGGTTCGTTGGCTTAAGCGGCGCCATTTTGTCCCAACTTTACCGTGCCTTTTATGGCAATAATCCCGAATCTCTTATCCTCTTAATCGCTTGGCTTCCCGCTGCCGTCTCTGTTGTTTTGCTCCGTTTCGTTCGCATCATTAAGGATCTCCGGCAGCCCAACGAGCTCAAAGTTTTTTACCATTTCCTTTACATTTCTCTCGGCCTTGCTGGGACTCTAATGGTTTTGATTATTTTGCAAAGTCTTCTCCGATTTCAACAAATACAATACGTCGGTAGTGCCATTGTTGTGATTGTTCTGCTTTTACTACCTCTGACCATTGTTTTCAGAGAGGAATTGAGTGTTTGGAAGAGTAAAATTGCAAGCCCAGTTTTACAATTAGAGTCGGCATCTCAACAACCACCGCCGCCACTGACTTCGACGGTGTCTCTTGCGCCATCGAGCCCTCCGTCGGAATCCTGCTTTAAAAACATGTTTAATCCTCCAAGCAGAGGAGAAGACTACACAATCCCACAAGCCATTTTCAGTATGGATTTGATAATTCTTTTTATGGCCACAATTTGTGGTGTCGGTGGAACATTGACGGCGATCGACAATTTGGGCCAAATCGGAGAGTCTTTGGGGTACCAATCTCACAGTATCACCACATTCATCTCTCTCGTCAGCATATGGAACTACCTCGGCCGTGTGGTCTCCGGATTCGTATCGGAATATTTCTGGAAAAAGTACAAAGTCCCACGTCCTCTGTTTCTCTTCGCGACACTTATTCTGTCCTGCGTCGGTCATCTTCTAATAGCATTCGGAGTTCCGAATTCATTGTATTTTTCTTCAATCGTAATCGGGTTCTGCTTCGGCGCACAGTGGCCATTGATCTATGCAATCATATCGGAGATTTTCGGGTTGAAGTACTACGCGACTTTGTCCAGTATGAGTGGAGGGGCGAGTCCGATTGGGGCATATATCTTGAACGTGAGAGTGGCGGGTCATTTGTACGACAGAGAGGCGCAAAGGCAGATGGAGGCAACGGGGCGACGGAGAAACATAGGAGAGGATTTGAGTTGCTTAGGAGTGGAATGTTACCGAAAGGCATTTCTTATAATCACGGCGGCGACGGTATTCGGGGCACTTGTTTCGCTGATTTTGGTGGTTCGGACATGGAAGTTCTATAAGGGCGATATTTACCGGAAGTTCAGAGAGGTGGAAGGTGAAGACATCGAAATGAAAATGGCCGCTCCGACCAACGGTACGTTGACGGCAGCGAAAAACTGAATCTGTTTAGTATATTTCTTAATGAGTATTTTCTCTACCAAATTAGAACTTTACTTATCATTGTACATTTTATCCAATCATCTTCCCATAGTTAGTTTCACAGTGAACTAATGTACACATGCATTAGGAGCCAACTTAAGGAATAAAAATGTACCACAACCAATACATCTTTGACATACTCAACAAATTATCTGATCTATGTGCTTGTTTTGTATATATATATATATATAT

Coding sequence (CDS)

ATGGCAGCCTCATCCTCAAATCCCACCTCCTCTAACCCCTTCTGGTACCAGTTTCTCTTTGGCCGCTGGTTCTCTGTCCTCGCTTCCATTCTCATCATGTCCGTCGCCGGTGCTACCTACATGTTCGCCCTCTACTCCTCCGACATCAAATCTTCCTTAGGCTACGACCAAACCACCCTCAATTTGCTCAGTTTCTTCAAGGACTTGGGCGGCAATGTTGGCGTCATCAGTGGTCTCACCAACGAGGTCGCTCCTGCCTGGGTCGTTCTTCTTATTGGTGCCGTCATGAACCTTTTTGGCTACACCATGATTTGGCTGGCCGTAACTAAAAGAATACCCAAACCCCAAATCTGGCATATGTGTCTCTATATCACCATCGGAGCTAATTCTCAGACCTTTGCAAATACTGGGGCTCTTGTAACCTGCGTCAAAAACTTCCCGGAAAGTCGTGGTAGTGTTTTGGGGCTTTTGAAGGGGTTCGTTGGCTTAAGCGGCGCCATTTTGTCCCAACTTTACCGTGCCTTTTATGGCAATAATCCCGAATCTCTTATCCTCTTAATCGCTTGGCTTCCCGCTGCCGTCTCTGTTGTTTTGCTCCGTTTCGTTCGCATCATTAAGGATCTCCGGCAGCCCAACGAGCTCAAAGTTTTTTACCATTTCCTTTACATTTCTCTCGGCCTTGCTGGGACTCTAATGGTTTTGATTATTTTGCAAAGTCTTCTCCGATTTCAACAAATACAATACGTCGGTAGTGCCATTGTTGTGATTGTTCTGCTTTTACTACCTCTGACCATTGTTTTCAGAGAGGAATTGAGTGTTTGGAAGAGTAAAATTGCAAGCCCAGTTTTACAATTAGAGTCGGCATCTCAACAACCACCGCCGCCACTGACTTCGACGGTGTCTCTTGCGCCATCGAGCCCTCCGTCGGAATCCTGCTTTAAAAACATGTTTAATCCTCCAAGCAGAGGAGAAGACTACACAATCCCACAAGCCATTTTCAGTATGGATTTGATAATTCTTTTTATGGCCACAATTTGTGGTGTCGGTGGAACATTGACGGCGATCGACAATTTGGGCCAAATCGGAGAGTCTTTGGGGTACCAATCTCACAGTATCACCACATTCATCTCTCTCGTCAGCATATGGAACTACCTCGGCCGTGTGGTCTCCGGATTCGTATCGGAATATTTCTGGAAAAAGTACAAAGTCCCACGTCCTCTGTTTCTCTTCGCGACACTTATTCTGTCCTGCGTCGGTCATCTTCTAATAGCATTCGGAGTTCCGAATTCATTGTATTTTTCTTCAATCGTAATCGGGTTCTGCTTCGGCGCACAGTGGCCATTGATCTATGCAATCATATCGGAGATTTTCGGGTTGAAGTACTACGCGACTTTGTCCAGTATGAGTGGAGGGGCGAGTCCGATTGGGGCATATATCTTGAACGTGAGAGTGGCGGGTCATTTGTACGACAGAGAGGCGCAAAGGCAGATGGAGGCAACGGGGCGACGGAGAAACATAGGAGAGGATTTGAGTTGCTTAGGAGTGGAATGTTACCGAAAGGCATTTCTTATAATCACGGCGGCGACGGTATTCGGGGCACTTGTTTCGCTGATTTTGGTGGTTCGGACATGGAAGTTCTATAAGGGCGATATTTACCGGAAGTTCAGAGAGGTGGAAGGTGAAGACATCGAAATGAAAATGGCCGCTCCGACCAACGGTACGTTGACGGCAGCGAAAAACTGA

Protein sequence

MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN*

Homology

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BLAST of CSPI03G33520.1 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 5.9e-29
Identity = 135/547 (24.68%), Postives = 247/547 (45.16%), Query Frame = 0


Query: 19  LFGRWFS---VLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGV 78
           +FGRW     ++A+I I +  G  + F+ YSS +KS LG  Q  LN L+   DLG   G 
Sbjct: 37  MFGRWRKWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGW 96

Query: 79  ISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQ--IWHMCLYITIGANSQTF 138
            SG+     P  VVL   A M   GY + WL +T  I  P   ++  CL   +   S  +
Sbjct: 97  SSGIALGYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCL---LAGLSICW 156

Query: 139 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAA 198
            NT   + C+++FP +R   L L   F G+S A+ S  + A   ++    +LL + +P  
Sbjct: 157 FNTACFILCIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLV 216

Query: 199 VSVVLLRFVRIIKDLRQPNELKVFYH----FLYISLGLAGTLMVLIILQSLLRFQQIQYV 258
           VS   L  V     L    +     H    F  +++    T   L++  S     ++ ++
Sbjct: 217 VSFAALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFI 276

Query: 259 GSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSL--APSSP 318
           G+ ++++  L  PL +  R+           PV+      +     + +   L    +S 
Sbjct: 277 GAVVLLVFPLCAPLLVYARDYF--------LPVINARLNHESSGYVMLNIDELKNQKTSV 336

Query: 319 PSESCFKNM-----FNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIG 378
            S++ +++M      N    G++++    I  ++  + ++A  CG    L   +NLGQI 
Sbjct: 337 SSKTGYEHMGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIA 396

Query: 379 ESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLL 438
           +SLG  S   TT +++ S +++ GR++S    ++  K++++ R  +    L+ + +   L
Sbjct: 397 QSLGQNS---TTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFL 456

Query: 439 IAF--GVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 498
           +A       +L  ++ +IG   G  +    +I S++FG        ++     PIG+ +L
Sbjct: 457 LAVSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LL 516

Query: 499 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 548
              +A  +Y+  A   +        + + + C+G +CY K F+     ++ G + SL L 
Sbjct: 517 YGYIAASIYEANASPDITPI-----VSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLY 562

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BLAST of CSPI03G33520.1 vs. ExPASy TrEMBL
Match: A0A0A0LEA4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G742600 PE=4 SV=1)

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0


Query: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60
           MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL
Sbjct: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120
           NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM
Sbjct: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120

Query: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180
           CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP
Sbjct: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180

Query: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240
           ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL
Sbjct: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240

Query: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300
           LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300

Query: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360
           SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ
Sbjct: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420
           IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH
Sbjct: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420

Query: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480
           LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL
Sbjct: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480

Query: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540
           NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN 581
           VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN
Sbjct: 541 VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN 580

[Close]

BLAST of CSPI03G33520.1 vs. ExPASy TrEMBL
Match: A0A5A7TKH4 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002830 PE=4 SV=1)

HSP 1 Score: 1034.2 bits (2673), Expect = 2.0e-298
Identity = 530/580 (91.38%), Postives = 550/580 (94.83%), Query Frame = 0


Query: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60
           MAAS+SNPTS+NPFWYQFLFGRWFSV ASILIMSVAGATYMFALYSSDIKSSLGYDQTTL
Sbjct: 1   MAASTSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120
           NLLSFFKDLGGNVGVISGL NEVAPAWVVLLIGAVMNLFGYTMIWLAVT RIPKPQIWHM
Sbjct: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120

Query: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180
           CLYI +GANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQL+ AFYGN+ 
Sbjct: 121 CLYICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDS 180

Query: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240
           +SLILLI WLPAAVSVV LRFVRIIKDLRQPNE+ VFYH LYISL LAGTLMVLIILQSL
Sbjct: 181 KSLILLIGWLPAAVSVVFLRFVRIIKDLRQPNEVNVFYHILYISLSLAGTLMVLIILQSL 240

Query: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300
           LRFQQIQYVGSAIVVIVLLLLPL IVFREELSVWKSKIA+P+LQLE ASQQPPPPLTSTV
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTV 300

Query: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360
            LAPSSPPS+SCFKNMF PP+RGEDYTIPQAIFS+D+IILF+ATICGVGGTLTAIDNLGQ
Sbjct: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420
           IGESL Y SHS TTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF TLILSC+GH
Sbjct: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGH 420

Query: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480
           LLIAFGVPNSLYFSSI+IGFCFGAQWPLI+AIISEIFGLKYYATL +M G ASPIGAYI+
Sbjct: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIM 480

Query: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540
           NVRVAGHLYDREAQRQMEA GR R  GEDLSCLGVECYRKAFLIITAATVFGALVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN 581
           VRTWKFYKGDIYR+FR  EGEDIEMKMAAPTN TLT AKN
Sbjct: 541 VRTWKFYKGDIYRRFRAEEGEDIEMKMAAPTNSTLTTAKN 580

[Close]

BLAST of CSPI03G33520.1 vs. ExPASy TrEMBL
Match: A0A6J1G7M8 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451560 PE=4 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 2.5e-272
Identity = 480/576 (83.33%), Postives = 523/576 (90.80%), Query Frame = 0


Query: 4   SSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLL 63
           ++SNP++ +P WYQFL GRWFSV ASILIMSV+GATYMF LYSSDIKSSLGYDQTTLNLL
Sbjct: 2   AASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNLL 61

Query: 64  SFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLY 123
           SFFKDLGGNVGVI GL NEVAP WVVLLIGAVMNLFGYTMIW+AVT RIPKPQIWHMCLY
Sbjct: 62  SFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCLY 121

Query: 124 ITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESL 183
           I IGANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGAIL+QLY AFYGNN +SL
Sbjct: 122 ICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKSL 181

Query: 184 ILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRF 243
           ILLIAWLPAAVSV  LRFVR+IKDLRQPNEL+VF+H LYISLGLAG+LMVLIILQ+ L+F
Sbjct: 182 ILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLKF 241

Query: 244 QQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSLA 303
            Q++Y GSAIVVI LLLLPL IV+REELSVWK+KI SP+ QLE ASQQ PPPLTS   +A
Sbjct: 242 HQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPVA 301

Query: 304 PSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGE 363
           P SPPS SCF+N F PP+RGEDYTIPQA+FS+D+IILF+ATICGVGGTLTAIDNLGQIGE
Sbjct: 302 PPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIGE 361

Query: 364 SLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 423
           SL Y S S TTFISLVSIWNYLGRVV+GF+SE+ WKKYKVPRPL  F T+ILSCVGHLLI
Sbjct: 362 SLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLLI 421

Query: 424 AFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVR 483
           AFGVPNSLYFSSI+IGFCFGAQWPLI+AIISEIFGLKYYATL ++ G ASPIGAYILNVR
Sbjct: 422 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVR 481

Query: 484 VAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRT 543
           VAGHLYDREA RQMEA GRRRNIGEDL+CLGVECYRKAFLIITAATV GA VSLILVVRT
Sbjct: 482 VAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVRT 541

Query: 544 WKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAK 580
           WKFYKGDIYRKFRE  GE++E++MAAPTN  +  ++
Sbjct: 542 WKFYKGDIYRKFREEAGEEVEVQMAAPTNSAVAKSQ 577

[Close]

BLAST of CSPI03G33520.1 vs. ExPASy TrEMBL
Match: A0A5A7TKY5 (Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G002840 PE=4 SV=1)

HSP 1 Score: 946.0 bits (2444), Expect = 7.2e-272
Identity = 482/571 (84.41%), Postives = 515/571 (90.19%), Query Frame = 0


Query: 9   TSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKD 68
           TS NPFWY  LFGRWFSV ASILIMSV+GATYMF LYSS IKSSL YDQTTLNLLSFFKD
Sbjct: 3   TSGNPFWYHLLFGRWFSVFASILIMSVSGATYMFGLYSSHIKSSLAYDQTTLNLLSFFKD 62

Query: 69  LGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGA 128
           LG N+GVISGL NEVAPAWVVLLIG VMNLFGYTMIWLAVT RIP PQIWHMCLYI IGA
Sbjct: 63  LGANIGVISGLVNEVAPAWVVLLIGVVMNLFGYTMIWLAVTNRIPNPQIWHMCLYICIGA 122

Query: 129 NSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIA 188
           NSQTFANT AL+TCVKNFPESRGS+LGL KGFVGLSGAILSQL+ AFYGNN +SLI LIA
Sbjct: 123 NSQTFANTAALITCVKNFPESRGSILGLFKGFVGLSGAILSQLFHAFYGNNSKSLIFLIA 182

Query: 189 WLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQY 248
           WLP+AVSV+L RFVRIIKDLRQPNELKVFYH LYISLGLAG+LMV IILQ+ LRFQQI Y
Sbjct: 183 WLPSAVSVILHRFVRIIKDLRQPNELKVFYHVLYISLGLAGSLMVCIILQNRLRFQQIHY 242

Query: 249 VGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSLAPSSPP 308
           VGSAIVVIVLLLLPL IVFREEL +W+SKI +P+ QLE ASQ PPPP        P +PP
Sbjct: 243 VGSAIVVIVLLLLPLAIVFREELRIWRSKIKNPIPQLELASQLPPPP--------PPNPP 302

Query: 309 SESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGESLGYQ 368
           S+SCFKNMFNPP+RGEDYTIPQAIFS+D+IILF+ATICGVGGTLTAIDNLGQIGESL Y 
Sbjct: 303 SDSCFKNMFNPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQIGESLRYP 362

Query: 369 SHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVP 428
           SHS TTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF TLILSC+GHLLIAFGVP
Sbjct: 363 SHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGHLLIAFGVP 422

Query: 429 NSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVRVAGHL 488
           NSLYFSSI+IGFCFGAQWPLI+AIISEIFGLKYYATL +M G ASPIGAYI+NVRVAGHL
Sbjct: 423 NSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIMNVRVAGHL 482

Query: 489 YDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYK 548
           YD EA+RQMEA GR R  GEDLSCLGVECY+KAFLIIT +TV G LVSLILVVRTWKFYK
Sbjct: 483 YDMEAERQMEAAGRLRKEGEDLSCLGVECYKKAFLIITGSTVLGGLVSLILVVRTWKFYK 542

Query: 549 GDIYRKFREVEGEDIEMKMAAPTNGTLTAAK 580
            DIYR+F+E EGEDIE+K+AAPTN +LTAAK
Sbjct: 543 DDIYRRFKEKEGEDIEIKIAAPTNNSLTAAK 565

[Close]

BLAST of CSPI03G33520.1 vs. ExPASy TrEMBL
Match: A0A6J1KSH4 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111498302 PE=4 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 3.0e-270
Identity = 477/576 (82.81%), Postives = 521/576 (90.45%), Query Frame = 0


Query: 4   SSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLL 63
           ++SNP++ +P WYQFL GRWFSV ASILIMSV+GATYMF LYSSDIKSSLGYDQTTLNLL
Sbjct: 2   AASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNLL 61

Query: 64  SFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLY 123
           SFFKDLGGNVGVI GL NEVAP WVVLLIGAVMNLFGYTMIW+AVT RIPKPQIWHMCLY
Sbjct: 62  SFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCLY 121

Query: 124 ITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESL 183
           I IGANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGAIL+QLY AFYGNN +SL
Sbjct: 122 ICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKSL 181

Query: 184 ILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRF 243
           ILLIAWLPAAVSV  LRFVR+IKDLRQPNEL+VF+H LYISLGLAG+LMVLIILQ+ L+F
Sbjct: 182 ILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLKF 241

Query: 244 QQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSLA 303
            Q++Y GSAIVVI LLLLPL IV+REELSVWK+KI SP+ QLE ASQ  PPPLTS   +A
Sbjct: 242 HQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPISQLELASQPQPPPLTSAAPVA 301

Query: 304 PSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGE 363
           P SPPS SCF+N F PP+RGEDYTI QA+FS+D+IILF+ATICGVGGTLTAIDNLGQIGE
Sbjct: 302 PPSPPSGSCFENTFKPPNRGEDYTILQAVFSIDMIILFIATICGVGGTLTAIDNLGQIGE 361

Query: 364 SLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 423
           SL Y S S TTFISLVSIWNYLGRVV+GF+SE+ WKKYKVPRPL  F T+ILSCVGHLLI
Sbjct: 362 SLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLLI 421

Query: 424 AFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVR 483
           AFGVPNSLYFSSI+IGFCFGAQWPLI+AIISEIFGLKYYATL ++ G ASPIGAYILNVR
Sbjct: 422 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVR 481

Query: 484 VAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRT 543
           VAGHLYDREA RQMEA GRRRNIGEDL+CLGVECYRKAFLIITAATV GA VSLILVVRT
Sbjct: 482 VAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVRT 541

Query: 544 WKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAK 580
           WKFYKGDIY+KFRE  GE++E++MAAPTN  +  ++
Sbjct: 542 WKFYKGDIYQKFREDSGEEVEVQMAAPTNSAVAKSQ 577

[Close]

BLAST of CSPI03G33520.1 vs. NCBI nr
Match: XP_011651970.1 (uncharacterized protein LOC101216551 [Cucumis sativus] >KGN58997.1 hypothetical protein Csa_001993 [Cucumis sativus])

HSP 1 Score: 1120.9 bits (2898), Expect = 0.0e+00
Identity = 580/580 (100.00%), Postives = 580/580 (100.00%), Query Frame = 0


Query: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60
           MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL
Sbjct: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120
           NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM
Sbjct: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120

Query: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180
           CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP
Sbjct: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180

Query: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240
           ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL
Sbjct: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240

Query: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300
           LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300

Query: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360
           SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ
Sbjct: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420
           IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH
Sbjct: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420

Query: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480
           LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL
Sbjct: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480

Query: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540
           NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN 581
           VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN
Sbjct: 541 VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN 580

[Close]

BLAST of CSPI03G33520.1 vs. NCBI nr
Match: KAA0043983.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK25157.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa])

HSP 1 Score: 1034.2 bits (2673), Expect = 4.1e-298
Identity = 530/580 (91.38%), Postives = 550/580 (94.83%), Query Frame = 0


Query: 1   MAASSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60
           MAAS+SNPTS+NPFWYQFLFGRWFSV ASILIMSVAGATYMFALYSSDIKSSLGYDQTTL
Sbjct: 1   MAASTSNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFALYSSDIKSSLGYDQTTL 60

Query: 61  NLLSFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHM 120
           NLLSFFKDLGGNVGVISGL NEVAPAWVVLLIGAVMNLFGYTMIWLAVT RIPKPQIWHM
Sbjct: 61  NLLSFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHM 120

Query: 121 CLYITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNP 180
           CLYI +GANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQL+ AFYGN+ 
Sbjct: 121 CLYICLGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNDS 180

Query: 181 ESLILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSL 240
           +SLILLI WLPAAVSVV LRFVRIIKDLRQPNE+ VFYH LYISL LAGTLMVLIILQSL
Sbjct: 181 KSLILLIGWLPAAVSVVFLRFVRIIKDLRQPNEVNVFYHILYISLSLAGTLMVLIILQSL 240

Query: 241 LRFQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTV 300
           LRFQQIQYVGSAIVVIVLLLLPL IVFREELSVWKSKIA+P+LQLE ASQQPPPPLTSTV
Sbjct: 241 LRFQQIQYVGSAIVVIVLLLLPLAIVFREELSVWKSKIANPILQLELASQQPPPPLTSTV 300

Query: 301 SLAPSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQ 360
            LAPSSPPS+SCFKNMF PP+RGEDYTIPQAIFS+D+IILF+ATICGVGGTLTAIDNLGQ
Sbjct: 301 PLAPSSPPSDSCFKNMFKPPNRGEDYTIPQAIFSIDMIILFIATICGVGGTLTAIDNLGQ 360

Query: 361 IGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGH 420
           IGESL Y SHS TTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLF TLILSC+GH
Sbjct: 361 IGESLRYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFVTLILSCIGH 420

Query: 421 LLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYIL 480
           LLIAFGVPNSLYFSSI+IGFCFGAQWPLI+AIISEIFGLKYYATL +M G ASPIGAYI+
Sbjct: 421 LLIAFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNMGGAASPIGAYIM 480

Query: 481 NVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540
           NVRVAGHLYDREAQRQMEA GR R  GEDLSCLGVECYRKAFLIITAATVFGALVSLILV
Sbjct: 481 NVRVAGHLYDREAQRQMEAAGRLRKKGEDLSCLGVECYRKAFLIITAATVFGALVSLILV 540

Query: 541 VRTWKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAKN 581
           VRTWKFYKGDIYR+FR  EGEDIEMKMAAPTN TLT AKN
Sbjct: 541 VRTWKFYKGDIYRRFRAEEGEDIEMKMAAPTNSTLTTAKN 580

[Close]

BLAST of CSPI03G33520.1 vs. NCBI nr
Match: XP_038905012.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 1003.4 bits (2593), Expect = 7.8e-289
Identity = 516/576 (89.58%), Postives = 537/576 (93.23%), Query Frame = 0


Query: 4   SSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLL 63
           ++SNPTS+NPFWYQFLFGRWFSV ASILIMSVAGATYMF LYSSDIKSSLGYDQTTLNLL
Sbjct: 2   AASNPTSANPFWYQFLFGRWFSVFASILIMSVAGATYMFGLYSSDIKSSLGYDQTTLNLL 61

Query: 64  SFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLY 123
           SFFKDLGGNVGVISGL NEVAPAWVVLLIGAVMNLFGYTMIWLAVT RIPKPQIWHMCLY
Sbjct: 62  SFFKDLGGNVGVISGLINEVAPAWVVLLIGAVMNLFGYTMIWLAVTNRIPKPQIWHMCLY 121

Query: 124 ITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESL 183
           I IGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQL+ AFYGNN +SL
Sbjct: 122 ICIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLFHAFYGNNSKSL 181

Query: 184 ILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRF 243
           I LIAWLPAAVSV  LRFVRIIKDLRQPNELKVFYH LYISLGLAG+LMVLIILQ+ LRF
Sbjct: 182 IFLIAWLPAAVSVTFLRFVRIIKDLRQPNELKVFYHILYISLGLAGSLMVLIILQNRLRF 241

Query: 244 QQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSLA 303
           QQIQYVGSAIVVIVLLLLPL IVFREEL+VWKSKIA+P+LQLE ASQQPP P      LA
Sbjct: 242 QQIQYVGSAIVVIVLLLLPLAIVFREELNVWKSKIANPILQLELASQQPPRP----PPLA 301

Query: 304 PSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGE 363
           P SPPS SCFKN F PP+RGEDYTIPQAIFS+D+IILF+ATICGVGGTLTAIDNLGQIGE
Sbjct: 302 PPSPPSGSCFKNTFKPPNRGEDYTIPQAIFSVDMIILFIATICGVGGTLTAIDNLGQIGE 361

Query: 364 SLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 423
           SLGY SHS TTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI
Sbjct: 362 SLGYPSHSTTTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 421

Query: 424 AFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVR 483
           AFGVPNSLYFSSIV+GFCFGAQWPLI+AIISEIFGLKYYATL ++ G ASPIGAYILNVR
Sbjct: 422 AFGVPNSLYFSSIVVGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGAASPIGAYILNVR 481

Query: 484 VAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRT 543
           VAGHLYDREAQRQMEA GRRRNIGEDLSCLGVECYRKAFLIITAATVFG LVSLILVVRT
Sbjct: 482 VAGHLYDREAQRQMEAAGRRRNIGEDLSCLGVECYRKAFLIITAATVFGGLVSLILVVRT 541

Query: 544 WKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAK 580
           WKFYK DIYRKFRE E ED+E+K+  P NG LTAAK
Sbjct: 542 WKFYKSDIYRKFREEEAEDVEIKIVTPANGALTAAK 573

[Close]

BLAST of CSPI03G33520.1 vs. NCBI nr
Match: KAG6596491.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 947.6 bits (2448), Expect = 5.1e-272
Identity = 480/576 (83.33%), Postives = 523/576 (90.80%), Query Frame = 0


Query: 4   SSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLL 63
           ++SNP++ +P WYQFL GRWFSV ASILIMSV+GATYMF LYSSDIKSSLGYDQTTLNLL
Sbjct: 2   AASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNLL 61

Query: 64  SFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLY 123
           SFFKDLGGNVGVI GL NEVAP WVVLLIGAVMNLFGYTMIW+AVT RIPKPQIWHMCLY
Sbjct: 62  SFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCLY 121

Query: 124 ITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESL 183
           I IGANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGAIL+QLY AFYGNN +SL
Sbjct: 122 ICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKSL 181

Query: 184 ILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRF 243
           ILLIAWLPAAVSV  LRFVR+IKDLRQPNEL+VF+H LYISLGLAG+LMVLIILQ+ L+F
Sbjct: 182 ILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLKF 241

Query: 244 QQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSLA 303
            Q++Y GSAIVVI LLLLPL IV+REELSVWK+KI SP+ QLE ASQQ PPPLTS   LA
Sbjct: 242 HQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPLA 301

Query: 304 PSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGE 363
           P SPPS SCF+N F PP+RGEDYTIPQA+FS+D+IILF+ATICGVGGTLTAIDNLGQIGE
Sbjct: 302 PPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIGE 361

Query: 364 SLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 423
           SL Y S S TTFISLVSIWNYLGRVV+GF+SE+ WKKYKVPRPL  F T+ILSCVGHLLI
Sbjct: 362 SLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLLI 421

Query: 424 AFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVR 483
           AFGVPNSLYFSSI+IGFCFGAQWPLI+AIISEIFGLKYYATL ++ G ASPIGAYILNV+
Sbjct: 422 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVK 481

Query: 484 VAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRT 543
           VAGHLYDREA RQMEA GRRRNIGEDL+CLGVECYRKAFLIITAATV GA VSLILVVRT
Sbjct: 482 VAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVRT 541

Query: 544 WKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAK 580
           WKFYKGDIYRKFRE  GE++E++MAAPTN  +  ++
Sbjct: 542 WKFYKGDIYRKFREEAGEEVEVQMAAPTNSAVAKSQ 577

[Close]

BLAST of CSPI03G33520.1 vs. NCBI nr
Match: XP_022947808.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 947.6 bits (2448), Expect = 5.1e-272
Identity = 480/576 (83.33%), Postives = 523/576 (90.80%), Query Frame = 0


Query: 4   SSSNPTSSNPFWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLL 63
           ++SNP++ +P WYQFL GRWFSV ASILIMSV+GATYMF LYSSDIKSSLGYDQTTLNLL
Sbjct: 2   AASNPSTVHPIWYQFLLGRWFSVFASILIMSVSGATYMFGLYSSDIKSSLGYDQTTLNLL 61

Query: 64  SFFKDLGGNVGVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLY 123
           SFFKDLGGNVGVI GL NEVAP WVVLLIGAVMNLFGYTMIW+AVT RIPKPQIWHMCLY
Sbjct: 62  SFFKDLGGNVGVIGGLINEVAPPWVVLLIGAVMNLFGYTMIWMAVTSRIPKPQIWHMCLY 121

Query: 124 ITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESL 183
           I IGANSQTFANTGALVT VKNFPESRGSVLGLLKGFVGLSGAIL+QLY AFYGNN +SL
Sbjct: 122 ICIGANSQTFANTGALVTSVKNFPESRGSVLGLLKGFVGLSGAILTQLYHAFYGNNSKSL 181

Query: 184 ILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRF 243
           ILLIAWLPAAVSV  LRFVR+IKDLRQPNEL+VF+H LYISLGLAG+LMVLIILQ+ L+F
Sbjct: 182 ILLIAWLPAAVSVTFLRFVRLIKDLRQPNELRVFFHILYISLGLAGSLMVLIILQNRLKF 241

Query: 244 QQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSLA 303
            Q++Y GSAIVVI LLLLPL IV+REELSVWK+KI SP+ QLE ASQQ PPPLTS   +A
Sbjct: 242 HQMEYAGSAIVVIALLLLPLAIVYREELSVWKTKIESPLSQLELASQQQPPPLTSAAPVA 301

Query: 304 PSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGE 363
           P SPPS SCF+N F PP+RGEDYTIPQA+FS+D+IILF+ATICGVGGTLTAIDNLGQIGE
Sbjct: 302 PPSPPSGSCFENTFKPPNRGEDYTIPQAVFSIDMIILFIATICGVGGTLTAIDNLGQIGE 361

Query: 364 SLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLI 423
           SL Y S S TTFISLVSIWNYLGRVV+GF+SE+ WKKYKVPRPL  F T+ILSCVGHLLI
Sbjct: 362 SLRYPSQSTTTFISLVSIWNYLGRVVAGFLSEFLWKKYKVPRPLIFFVTMILSCVGHLLI 421

Query: 424 AFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVR 483
           AFGVPNSLYFSSI+IGFCFGAQWPLI+AIISEIFGLKYYATL ++ G ASPIGAYILNVR
Sbjct: 422 AFGVPNSLYFSSIIIGFCFGAQWPLIFAIISEIFGLKYYATLYNLGGVASPIGAYILNVR 481

Query: 484 VAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRT 543
           VAGHLYDREA RQMEA GRRRNIGEDL+CLGVECYRKAFLIITAATV GA VSLILVVRT
Sbjct: 482 VAGHLYDREATRQMEAAGRRRNIGEDLNCLGVECYRKAFLIITAATVLGAFVSLILVVRT 541

Query: 544 WKFYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAK 580
           WKFYKGDIYRKFRE  GE++E++MAAPTN  +  ++
Sbjct: 542 WKFYKGDIYRKFREEAGEEVEVQMAAPTNSAVAKSQ 577

[Close]

BLAST of CSPI03G33520.1 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 697.2 bits (1798), Expect = 1.1e-200
Identity = 363/574 (63.24%), Postives = 439/574 (76.48%), Query Frame = 0


Query: 17  QFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGVI 76
           Q L GRWF    S+LIMS AGATYMF +YS DIK +LGYDQTTLNLLSFFKDLG NVGV+
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 77  SGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTFANT 136
           +GL NEV P W +LLIGA++N FGY MIWLAVT+RI KPQ+WHMCLYI +GANSQ+FANT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 137 GALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAAVSV 196
           G+LVTCVKNFPESRG VLG+LKG+VGLSGAI++QLYRAFYG + + LIL+I WLPA VS 
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWLPAIVSF 195

Query: 197 VLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGSAIVVI 256
             LR +RI+K  RQ NELKVFY+FLYISLGLA  LMV+II+  L  F Q ++ GSA VVI
Sbjct: 196 AFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVVI 255

Query: 257 VLLLLPLTIVFREELSVWKSK-----------IASPVLQLESASQQPPPPLTSTVSLAPS 316
           VLLLLP+ +V  EE  +WK K           + +   +L+S+  +      S   +   
Sbjct: 256 VLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEKV 315

Query: 317 SPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGESL 376
             P  SC+  +FNPP RG+DYTI QA+FS+D++ILF+ATICGVGGTLTAIDNLGQIG SL
Sbjct: 316 KTP--SCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSL 375

Query: 377 GYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAF 436
           GY   S++TF+SLVSIWNY GRVVSG VSE F  KYK PRPL L   L+LSC GHLLIAF
Sbjct: 376 GYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAF 435

Query: 437 GVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVRVA 496
            VP  LY +S++IGFCFGAQWPL++AIISEIFGLKYY+TL +    ASPIG+Y+LNVRVA
Sbjct: 436 NVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVA 495

Query: 497 GHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWK 556
           G+LYD EA +Q +A G+ R  G+DL+C+G  C++ +F+II A T+FG LVS++LV+RT K
Sbjct: 496 GYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKK 555

Query: 557 FYKGDIYRKFREVEGEDIEMKMAAPTNGTLTAAK 580
           FYK DIY+KFRE +    EM+MAAP     T AK
Sbjct: 556 FYKSDIYKKFRE-KALAAEMEMAAPAAARSTVAK 586

[Close]

BLAST of CSPI03G33520.1 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 629.4 bits (1622), Expect = 2.9e-180
Identity = 331/556 (59.53%), Postives = 409/556 (73.56%), Query Frame = 0


Query: 14  FWYQFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNV 73
           F   F  GRWF V AS LIM+ AGATY+F  YS DIKS+LGYDQTTLNLL FFKDLG NV
Sbjct: 9   FLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANV 68

Query: 74  GVISGLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTF 133
           GV+SGL  EV P W VL IG+ MN  GY MIWL VT ++ KP++W MCLYI IGANSQ F
Sbjct: 69  GVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNF 128

Query: 134 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAA 193
           ANTGALVTCVKNFPESRG +LGLLKG+VGLSGAI +QLY A YG++ +SLILLIAWLPAA
Sbjct: 129 ANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAA 188

Query: 194 VSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGSAI 253
           VS+V +  +R  K +RQ NEL VFY FLYIS+ LA  LM + I +  + F +  Y  SA 
Sbjct: 189 VSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASAT 248

Query: 254 VVIVLLLLPLTIVFREELSVW---KSKIASP-VLQLESASQQPPPPLTSTVSLAPSSPPS 313
           +   LL +PLT+  ++EL VW   K  I  P  +++E   ++          +      +
Sbjct: 249 ICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNGEEKET 308

Query: 314 ESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGESLGYQS 373
           +SCF  +F+PP RGEDYTI QA+ S D+IILF+AT CG+G +LTA+DNLGQIGESLGY +
Sbjct: 309 KSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESLGYPN 368

Query: 374 HSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVPN 433
           H++++F+SLVSIWNY GRV SGFVSEY   KYK+PRPL +   L+LSC GHLLIAF VP 
Sbjct: 369 HTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAFPVPG 428

Query: 434 SLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVRVAGHLY 493
           S+Y +SI++GF FGAQ PL++AIISE+FGLKYY+TL +    ASP+G+YILNVRV G LY
Sbjct: 429 SVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVTGMLY 488

Query: 494 DREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYKG 553
           D+EA +Q+ A G  R   +DL+CLG +CY+  FLI+ A T FGALVSL L +RT +FYKG
Sbjct: 489 DKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIRTREFYKG 548

Query: 554 DIYRKFREVEGEDIEM 566
           DIY+KFRE    + E+
Sbjct: 549 DIYKKFRESPESESEL 564

[Close]

BLAST of CSPI03G33520.1 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 328.6 bits (841), Expect = 1.0e-89
Identity = 202/545 (37.06%), Postives = 313/545 (57.43%), Query Frame = 0


Query: 22  RWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGVISGL-- 81
           +W +  ASI I S +GATY FA+YSS +KSS  YDQ+TL+ +S FKD+GG  G+ISG   
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 82  ------TNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTF 141
                 +      WVV+ +G V    G+  IW +V   I  P +  MCL++ +  +S  F
Sbjct: 67  TAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPF 126

Query: 142 ANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYG--NNPESLILLIAWLP 201
            NT  +VT  +NF +  G+ +G+++GF+GLSGAIL QLY A  G   NP + ILL+A +P
Sbjct: 127 FNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVP 186

Query: 202 AAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGS 261
             V  + + FVR+ + +   ++ K       IS+ +A  LMV+I ++++L   +   + S
Sbjct: 187 TLVMFLAMPFVRVYETV-TISDKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQIFS 246

Query: 262 AIVVIVLLLLPLTIVFR--EELSVWKSKIASPVLQLESASQQPPPPLTSTVSLAPSSPPS 321
            I+V++LL  PL +  R   E     S +  PVL                 + A   PPS
Sbjct: 247 FILVLLLLASPLLVAVRALREKRQTLSSLDGPVLD----------------TSALLDPPS 306

Query: 322 ESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIGESLGYQS 381
            + F +        ED  I +A+ +++  +LF+A +CG+G     ++N+ QIGESL Y S
Sbjct: 307 SNIFPD--GDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSS 366

Query: 382 HSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLLIAFGVPN 441
             + + +SL SIWN+LGR  +G+VS+ F  K+  PRP+F+  TL +  +GH+++A GV  
Sbjct: 367 VQLNSLVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQG 426

Query: 442 SLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNVRVAGHLY 501
           SLY  S++IG  +G+QW L+  I SEIFG+++  T+      A PIG+YIL+V+V G+ Y
Sbjct: 427 SLYAGSVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFY 486

Query: 502 DREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVRTWKFYKG 555
           D+ A              +D SC G +C+R +F+I+ +  +FG+LV+ +L  RT KFYK 
Sbjct: 487 DKVASE------------DDNSCFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYKN 520

[Close]

BLAST of CSPI03G33520.1 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 327.8 bits (839), Expect = 1.8e-89
Identity = 212/576 (36.81%), Postives = 315/576 (54.69%), Query Frame = 0


Query: 18  FLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGVIS 77
           F+  RW   +A++ I S AG  Y+F   S  IKSSL Y+Q  L+ L   KDLG +VG I+
Sbjct: 12  FINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIA 71

Query: 78  GLTNEVAPAWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCLYITIGANSQTFANTG 137
           G  +E+ P W  LL+GAV NL GY  +WL VT R P   +W MC+ I +G N +T+ NTG
Sbjct: 72  GTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTG 131

Query: 138 ALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPESLILLIAWLPAAVSVV 197
           ALV+ V+NFP+SRG V+G+LKGF GL GAI+SQ+Y   + +NP SLIL++A  PA V V 
Sbjct: 132 ALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHSSNPASLILMVAVTPAVVVVC 191

Query: 198 LLRFVRIIKDLRQ--PNELKVFYHFLYISLGLAGTLMVLIILQSLLRFQQIQYVGSAIVV 257
           L+ F+R +   +Q  P +   F     + L LA  LM ++++Q L+           IV+
Sbjct: 192 LMFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVL 251

Query: 258 IVLLLLPLTIVFR--------------EELSVWKSKIASPVLQ-----LESASQQPPPPL 317
            V+L++P+ +                 EE  V K +   P LQ     L     + P   
Sbjct: 252 FVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKP--- 311

Query: 318 TSTVSLAPSS---------------PPSESCFK-NMFNPPSRGEDYTIPQAIFSMDLIIL 377
              V L P+S                 +E   + N    P RGED+T+ QA+   D  ++
Sbjct: 312 -KDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLI 371

Query: 378 FMATICGVGGTLTAIDNLGQIGESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKK 437
           F + + G G  LT IDNLGQ+ +SLGY +  +   +S++SIWN+LGR+  G+ SE   + 
Sbjct: 372 FFSLLLGSGSGLTVIDNLGQMSQSLGYDNTHV--LVSMISIWNFLGRIGGGYFSELVVRD 431

Query: 438 YKVPRPLFLFATLILSCVGHLLIAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLK 497
           Y  PRP+ +    ++  VGH+  A+G P ++Y  +++IG  +GA W ++ A  SE+FGLK
Sbjct: 432 YAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLK 491

Query: 498 YYATLSSMSGGASPIGAYILNVRVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRK 557
            +  L +    A+P G+ + +  +A  +YDREA+RQ  A G   +  + L C G  C+  
Sbjct: 492 KFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQ--AHGSVFDPDDALRCNGSICFFL 551

[Close]

BLAST of CSPI03G33520.1 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 324.7 bits (831), Expect = 1.5e-88
Identity = 201/546 (36.81%), Postives = 310/546 (56.78%), Query Frame = 0


Query: 17  QFLFGRWFSVLASILIMSVAGATYMFALYSSDIKSSLGYDQTTLNLLSFFKDLGGNVGVI 76
           + L  +W ++ ASI I   AG +Y F +YS+ +KS+  YDQ+TL+ +S FKD+GGNVGV+
Sbjct: 4   EILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVL 63

Query: 77  SGLTNEVAP--------------AWVVLLIGAVMNLFGYTMIWLAVTKRIPKPQIWHMCL 136
           SGL    A                WVV+LIGA++N  GY ++W +VT  I +P +  MCL
Sbjct: 64  SGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCL 123

Query: 137 YITIGANSQTFANTGALVTCVKNFPESRGSVLGLLKGFVGLSGAILSQLYRAFYGNNPES 196
           ++ I A S TF NT  +V+ ++NF +  G+ +G++KGFVGLSGA+L QLY      +P++
Sbjct: 124 FMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKT 183

Query: 197 LILLIAWLPAAVSVVLLRFVRIIKDLRQPNELKVFYHFLYISLGLAGTLMVLIILQSLLR 256
            ILL+A +P+ +SV+++  VR+ K     +E K       +SL +A  LM+ IIL+S L 
Sbjct: 184 FILLLAIVPSLLSVLVMPLVRVYK-TSTVDEKKHLDGLSTLSLIIAAYLMITIILKSTLS 243

Query: 257 FQQIQYVGSAIVVIVLLLLPLTIVFREELSVWKSKIASPVLQLESASQQPPPPLTSTVSL 316
                   +  V++VLL  PL +  R      +  I  P+  + S       PL   +  
Sbjct: 244 LPSWANAVTLAVLLVLLSSPLLVAVRAH----RDSIEKPLSSVYS-------PLVDNL-- 303

Query: 317 APSSPPSESCFKNMFNPPSRGEDYTIPQAIFSMDLIILFMATICGVGGTLTAIDNLGQIG 376
                  E+            +   + QA+ ++D  +LF+A ICG+G  ++ I+N+ QIG
Sbjct: 304 -------EATTSGEILMLDEDKSLNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIG 363

Query: 377 ESLGYQSHSITTFISLVSIWNYLGRVVSGFVSEYFWKKYKVPRPLFLFATLILSCVGHLL 436
           ESL Y S  I + ++L +IWN++GR   G+VS++   +   PRPL +  TL    +GHL+
Sbjct: 364 ESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLI 423

Query: 437 IAFGVPNSLYFSSIVIGFCFGAQWPLIYAIISEIFGLKYYATLSSMSGGASPIGAYILNV 496
           IA G   +LY  SI++G C+G+QW L+  I SE+FG+K+  T+ +    ASP+G+YI +V
Sbjct: 424 IASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSV 483

Query: 497 RVAGHLYDREAQRQMEATGRRRNIGEDLSCLGVECYRKAFLIITAATVFGALVSLILVVR 549
           R+ G++YDR              IGE  +C G  C+R A+++I +    G LVS +LV R
Sbjct: 484 RLIGYIYDRTI------------IGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFR 516

The following BLAST results are available for this feature:

BLAST of CSPI03G33520.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	5.9e-29	24.68	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]

BLAST of CSPI03G33520.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LEA4	0.0e+00	100.00	Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G74260...	[more]
A0A5A7TKH4	2.0e-298	91.38	Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1G7M8	2.5e-272	83.33	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A5A7TKY5	7.2e-272	84.41	Protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1KSH4	3.0e-270	82.81	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111498...	[more]

BLAST of CSPI03G33520.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651970.1	0.0e+00	100.00	uncharacterized protein LOC101216551 [Cucumis sativus] >KGN58997.1 hypothetical ...	[more]
KAA0043983.1	4.1e-298	91.38	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucumis melo var. makuwa] >TYK25157.1 p...	[more]
XP_038905012.1	7.8e-289	89.58	protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida]	[more]
KAG6596491.1	5.1e-272	83.33	Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022947808.1	5.1e-272	83.33	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata]	[more]

BLAST of CSPI03G33520.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39210.1	1.1e-200	63.24	Major facilitator superfamily protein	[more]
AT2G28120.1	2.9e-180	59.53	Major facilitator superfamily protein	[more]
AT2G34355.1	1.0e-89	37.06	Major facilitator superfamily protein	[more]
AT5G14120.1	1.8e-89	36.81	Major facilitator superfamily protein	[more]
AT1G18940.1	1.5e-88	36.81	Nodulin-like / Major Facilitator Superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 22..267 e-value: 1.3E-92 score: 309.8
IPR011701	Major facilitator superfamily	PFAM	PF07690	MFS_1	coord: 344..539 e-value: 1.0E-9 score: 37.9
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 331..546 e-value: 1.7E-11 score: 45.9
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 222..545
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 8..207
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 287..306
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 10..572
None	No IPR available	PANTHER	PTHR21576:SF122	MFS TRANSPORTER	coord: 10..572
None	No IPR available	CDD	cd17354	MFS_Mch1p_like	coord: 21..533 e-value: 3.54947E-111 score: 335.375

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G33520	CSPI03G33520	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G33520.1.utr5p1	CSPI03G33520.1.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G33520.1.cds1	CSPI03G33520.1.cds1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G33520.1.utr3p1	CSPI03G33520.1.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G33520.1	CSPI03G33520.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
biological_process	GO:0099587	inorganic ion import across plasma membrane
biological_process	GO:0002239	response to oomycetes
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0022857	transmembrane transporter activity