CSPI03G28610.1 (mRNA) Cucumber (PI 183967) v1

BLAST of CSPI03G28610.1 vs. ExPASy Swiss-Prot
Match: Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 5.5e-205
Identity = 362/586 (61.77%), Postives = 453/586 (77.30%), Query Frame = 0

Query: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60
           MDT+KSFKGYGKV+ELEQQA+ +KTR+RLIII +S ++LIA+++ AV G+V+H RNS SS
Sbjct: 1   MDTIKSFKGYGKVNELEQQAYEKKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESS 60

Query: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSC---QSSLQNSNTTDPVFLFKLSLRVATDSL 120
            ++ S P TEL+P ASLK +C  T+YPSSC    SSL  SNTTDP  LFKLSLRVA D  
Sbjct: 61  PSSDSVPQTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAID-- 120

Query: 121 SKLSDYTSNFNSTT-GDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRI 180
            +LS + S   +    D +++ AI +C SVF DA+D LND++S++     + +  S + +
Sbjct: 121 -ELSSFPSKLRANAEQDARLQKAIDVCSSVFGDALDRLNDSISAL--GTVAGRIASSASV 180

Query: 181 EDLKTWLSTTITDQETCLDALRDLNQTT---VLQDLQTAMVNSTEFTSNSLAIVTKILGL 240
            +++TWLS  +TDQ+TCLDA+ +LN T     LQ+++TAM NSTEF SNSLAIVTKILGL
Sbjct: 181 SNVETWLSAALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGL 240

Query: 241 LADFNIPI-HRKLMGLPEWVSSGDRRLLQE--NNVTAHVTVSKDGKGQYTTIQDAVAAVP 300
           L+ F  PI HR+L+G PEW+ + +RRLL+E  N+ T    V+KDG GQ+ TI +A+  V 
Sbjct: 241 LSRFETPIHHRRLLGFPEWLGAAERRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVK 300

Query: 301 KKSKERFIIHVKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFA 360
           KKS+ERF ++VKEG Y EN+ LDK+ WNVM+YGDG+ KT V G  NF+DGTPTF TATFA
Sbjct: 301 KKSEERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFA 360

Query: 361 VAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDC 420
           V GKGFI KD+GF+N AG +KHQAVA RSGSD SV   CSFDG+QDTLYAHSNRQFYRDC
Sbjct: 361 VKGKGFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDC 420

Query: 421 DITGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYN 480
           DITGTIDFIFGNAAVVFQ+C I PRQPLPNQFNTITAQGKKD NQN+GI IQK T + + 
Sbjct: 421 DITGTIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFG 480

Query: 481 DSLNAPTYLGRPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGS 540
           ++L APTYLGRPWK+FSTTVIM+S+IG  LNPVGW  WV   +PP++IFY EYQNSGPG+
Sbjct: 481 NNLTAPTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGA 540

Query: 541 NVDKRVRWAGYKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 577
           +V +RV+WAGYKP++TD  A  FTV +F+ G +WLP   V FD++L
Sbjct: 541 DVSQRVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581

BLAST of CSPI03G28610.1 vs. ExPASy Swiss-Prot
Match: Q43867 (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 5.0e-198
Identity = 356/590 (60.34%), Postives = 443/590 (75.08%), Query Frame = 0

Query: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60
           MD+V SFKGYGKVDE +  A ++KTR+RL+++ IS+++LIA+++ AVV  VVHK  + S 
Sbjct: 1   MDSVNSFKGYGKVDEAQDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNES- 60

Query: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSS---LQNSNTTDPVFLFKLSLRVATDSL 120
             T SPPP ELTP  SLK +CSVT++P SC SS   L +SNTTDP  LFKLSL+V  D L
Sbjct: 61  --TPSPPP-ELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDEL 120

Query: 121 SKLSDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIE 180
             +SD     +  T D ++++A+++C  + EDA+D LNDTVS+++ D   +K LS S+IE
Sbjct: 121 DSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAID-DEEKKKTLSSSKIE 180

Query: 181 DLKTWLSTTITDQETCLDALRDLNQ-------TTVLQDLQTAMVNSTEFTSNSLAIVTKI 240
           DLKTWLS T+TD ETC D+L +L Q       +T+ Q+L++AM  STEFTSNSLAIV+KI
Sbjct: 181 DLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKI 240

Query: 241 LGLLADFNIPIHRK----------LMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYT 300
           L  L+D  IPIHR+           +   +W     RRLLQ   +   VTV+ DG G   
Sbjct: 241 LSALSDLGIPIHRRRRLMSHHHQQSVDFEKWAR---RRLLQTAGLKPDVTVAGDGTGDVL 300

Query: 301 TIQDAVAAVPKKSKERFIIHVKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDG 360
           T+ +AVA VPKKS + F+I+VK G Y ENV++DKSKWNVM+YGDG+ KTI+SG  NF+DG
Sbjct: 301 TVNEAVAKVPKKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDG 360

Query: 361 TPTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYA 420
           TPT+ TATFA+ GKGFI KD+G INTAG AKHQAVAFRSGSD SV   CSFDG+QDTLY 
Sbjct: 361 TPTYETATFAIQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYP 420

Query: 421 HSNRQFYRDCDITGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGIS 480
           HSNRQFYRDCD+TGTIDFIFG+AAVVFQ C I PRQPL NQFNTITAQGKKD NQ+SG+S
Sbjct: 421 HSNRQFYRDCDVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMS 480

Query: 481 IQKCTFSAYNDSLNAPTYLGRPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFY 540
           IQ+CT SA N ++ APTYLGRPWKEFSTTVIM + IG  + P GW  WVSG DPP+SI Y
Sbjct: 481 IQRCTISA-NGNVIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVY 540

Query: 541 GEYQNSGPGSNVDKRVRWAGYKPSLTDSEAGRFTVGTFLNGEDWLPATNV 571
           GEY+N+GPGS+V +RV+WAGYKP ++D+EA +FTV T L+G DW+PAT V
Sbjct: 541 GEYKNTGPGSDVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGV 581

BLAST of CSPI03G28610.1 vs. ExPASy Swiss-Prot
Match: Q9LUL8 (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3702 GN=PME26 PE=2 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 7.3e-157
Identity = 294/528 (55.68%), Postives = 370/528 (70.08%), Query Frame = 0

Query: 52  VHKRNS-SSSSTTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQ----NSNTTDPVFLF 111
           +H+R+   S     SP P   TP + L+T+C+VT YP+SC SS+     +  TTDP  LF
Sbjct: 436 IHERHGVQSPRLRKSPHP---TPSSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLF 495

Query: 112 KLSLRVATDSLSKLSDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSM-EVDR 171
           +LSL+V  D L+ +          T D  +++A+ +C  VF+ A+D++NDT+SS+ EV  
Sbjct: 496 RLSLQVTFDELNSIVGLPKKLAEETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVIS 555

Query: 172 HSEKFLSPSRIEDLKTWLSTTITDQETCLDALRDLN-QTTVLQDLQTAMVNSTEFTSNSL 231
             +K L+ S I DL TWLS+ +TD  TC D L + N  + + Q L++AMVNSTEFTSNSL
Sbjct: 556 GGKKNLNSSTIGDLITWLSSAVTDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSL 615

Query: 232 AIVTKILGLLADFNIPIH-RKLM---GLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYT 291
           AIV ++L   +   IP+  R+L+     P WV  G RRLLQ  N+T HVTV+ DG G   
Sbjct: 616 AIVAQVLKKPSKSRIPVQGRRLLNSNSFPNWVRPGVRRLLQAKNLTPHVTVAADGSGDVR 675

Query: 292 TIQDAVAAVPKKSKERFIIHVKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDG 351
           T+ +AV  VPKK K  F+I+VK G Y ENV++ K KWNV +YGDGR KTI+SG  N +DG
Sbjct: 676 TVNEAVWRVPKKGKTMFVIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDG 735

Query: 352 TPTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYA 411
             TF+T+TFA  GKGF+ KDMG INTAGP KHQAVAFRS SD SV   CSFDGYQDTLY 
Sbjct: 736 VRTFNTSTFATEGKGFMMKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYT 795

Query: 412 HSNRQFYRDCDITGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGIS 471
           HSNRQ+YR+CD+TGT+DFIFG   VVFQ C+IRPRQPLPNQFNTITA+G ++ NQN+GIS
Sbjct: 796 HSNRQYYRNCDVTGTVDFIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGIS 855

Query: 472 IQKCTFSAYNDSLNAPTYLGRPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQD-PPSSIF 531
           I +CT S  N ++ A TYLGRPWK FS TVIM+S IG F+NP GW  W S  D PP +IF
Sbjct: 856 IHQCTISP-NGNVTATTYLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIF 915

Query: 532 YGEYQNSGPGSNVDKRVRWAGYKPSLTDSEAGRFTVGTFLNGED-WLP 567
           Y EY+NSGPGS++ KRV+WAGYKP  +D EA RFTV  FL G+D W+P
Sbjct: 916 YREYKNSGPGSDLSKRVKWAGYKPISSDDEAARFTVKYFLRGDDNWIP 959

BLAST of CSPI03G28610.1 vs. ExPASy Swiss-Prot
Match: Q9FF78 (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 1.9e-136
Identity = 277/579 (47.84%), Postives = 376/579 (64.94%), Query Frame = 0

Query: 10  YGKVDELEQQAFR--QKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSSSTTTSPP 69
           YG++DE EQ      +KT++R+ II IS ++L+ ++VGAVVG    + NS    T  +  
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTA-RDNSKKPPTENNGE 63

Query: 70  PTELTPPASLKTLCSVTQYPSSCQSSL---QNSNTTDPVFLFKLSLRVATDSLSKLSDYT 129
           P  +    S+K LC VT +   C  +L    N++ + P  LFK +++V    LSK+ D  
Sbjct: 64  PISV----SVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGF 123

Query: 130 SNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLKTWLS 189
           SN      D    AA+  C  +   A+D LN+T++S           S    +DL+TWLS
Sbjct: 124 SNGEHM--DNATSAAMGACVELIGLAVDQLNETMTS-----------SLKNFDDLRTWLS 183

Query: 190 TTITDQETCLDALRDLNQTTVLQDLQTAMVNSTEFTSNSLAIVTKILGLLADFNIPIHRK 249
           +  T QETC+DAL + N+ ++    +  + NSTE TSN+LAI+T  LG +AD      R+
Sbjct: 184 SVGTYQETCMDALVEANKPSLTTFGENHLKNSTEMTSNALAIIT-WLGKIADTVKFRRRR 243

Query: 250 LMG-------LPEWVSSGDRRLLQENNV--TAHVTVSKDGKGQYTTIQDAVAAVPKKSKE 309
           L+        + +      RRLL+  ++   A + V+KDG G+Y TI +A+A V +K+++
Sbjct: 244 LLETGNAKVVVADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEK 303

Query: 310 RFIIHVKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKG 369
             II+VK+G+Y ENV ++K+KWNV+M GDG++KTIVS  LNFIDGTPTF TATFAV GKG
Sbjct: 304 PTIIYVKKGVYLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKG 363

Query: 370 FIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGT 429
           F+ +DMGFINTAGPAKHQAVA    +DLSV   C+ D +QDT+YAH+ RQFYRDC I GT
Sbjct: 364 FMARDMGFINTAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGT 423

Query: 430 IDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNA 489
           +DFIFGNAAVVFQ C I PR+P+  Q NTITAQG+KD NQN+GISI  CT    ++  + 
Sbjct: 424 VDFIFGNAAVVFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDI 483

Query: 490 PTYLGRPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKR 549
            T+LGRPWK+FSTTVIM+S +  F+NP GW  W +G   P +IFY EY NSGPG++   R
Sbjct: 484 QTFLGRPWKDFSTTVIMKSFMDKFINPKGWLPW-TGDTAPDTIFYAEYLNSGPGASTKNR 543

Query: 550 VRWAGYKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDT 575
           V+W G K SLT  EA +FTV  F++G +WLPAT V F++
Sbjct: 544 VKWQGLKTSLTKKEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of CSPI03G28610.1 vs. ExPASy Swiss-Prot
Match: Q9SG77 (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.9e-128
Identity = 258/580 (44.48%), Postives = 372/580 (64.14%), Query Frame = 0

Query: 10  YGKVDELEQQAF--RQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSSSTTTSPP 69
           YGKVDE E      R+KTR+ + II +SL++L  +++GAV G + HK++  +  T  +  
Sbjct: 5   YGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNGD 64

Query: 70  PTELTPPASLKTLCSVTQYPSSCQSSL---QNSNTTDPVFLFKLSLRVATDSLSKLSDYT 129
              +    S+K +C VT +   C  +L    N+++ +P  LF+ ++++   +++++S   
Sbjct: 65  SISV----SVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKI---TIAEVSKAI 124

Query: 130 SNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLKTWLS 189
           + F+S+ GD K    +  C  + +  ID LN+T++S      +     P  ++DL+TWLS
Sbjct: 125 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSS----NGDVTVPELVDDLRTWLS 184

Query: 190 TTITDQETCLDALRDLNQTTVLQDL----QTAMVNSTEFTSNSLAIVTKILGLLADFNIP 249
           +  T Q TC++        T+  D+    ++ + NSTE TSN+LAI+T  LG +AD +  
Sbjct: 185 SAGTYQRTCVE--------TLAPDMRPFGESHLKNSTELTSNALAIIT-WLGKIAD-SFK 244

Query: 250 IHRKLMGLP--EWVSSGDRRLLQENNV--TAHVTVSKDGKGQYTTIQDAVAAVPKKSKER 309
           + R+L+     E      RRLLQ  ++   A + V+KDG G+Y TI+ A+  VP+KS++R
Sbjct: 245 LRRRLLTTADVEVDFHAGRRLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKR 304

Query: 310 FIIHVKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGF 369
            II+VK+G+Y ENV ++K  WNV++ GDG +K+IVSG LN IDGTPTF TATFAV GKGF
Sbjct: 305 TIIYVKKGVYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGF 364

Query: 370 IGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTI 429
           + +DMGFINTAGP+KHQAVA    +DL+    C+ + YQDTLY H+ RQFYR+C I GT+
Sbjct: 365 MARDMGFINTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTV 424

Query: 430 DFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAP 489
           DFIFGN+A V Q+C I PR+P+  Q NTITAQG+ D N N+GISI +C  S   D  +  
Sbjct: 425 DFIFGNSASVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVM 484

Query: 490 TYLGRPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRV 549
           T+LGRPWK FSTTVIM S + GF++  GW  W +G   P +IFYGEY+N+GPG++   RV
Sbjct: 485 TFLGRPWKNFSTTVIMDSYLHGFIDRKGWLPW-TGDSAPDTIFYGEYKNTGPGASTKNRV 544

Query: 550 RWAGYKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 577
           +W G +  L+  EA RFTV  F++G  WLPAT V F + L
Sbjct: 545 KWKGLR-FLSTKEANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of CSPI03G28610.1 vs. ExPASy TrEMBL
Match: A0A0A0L956 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646620 PE=3 SV=1)

HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 575/576 (99.83%), Postives = 575/576 (99.83%), Query Frame = 0

Query: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60
           MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60

Query: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120
           STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180
           SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMVNSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDALRDLNQTTVLQDLQTAM NSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300
           IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480

Query: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540
           RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG
Sbjct: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 577
           YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL
Sbjct: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 576

BLAST of CSPI03G28610.1 vs. ExPASy TrEMBL
Match: A0A1S3CBU9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499202 PE=3 SV=1)

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 559/576 (97.05%), Postives = 572/576 (99.31%), Query Frame = 0

Query: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60
           MDTVKSFKGYGKVDELEQQAFRQKTRRRLIII IS++LLIALL+GAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120
           STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180
           SDYTSNFNS TGDPKV+AAIKIC+SVFEDAIDTLNDT+SSMEVDRHSEKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMVNSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDALRDLNQTTVLQDLQTAM NSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300
           IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKG YTTI+DAVAAVPKKSKERFIIH
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYN+SL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540
           RPWKEFSTTVIM+SEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 577
           YKPSLTDSEAG+FTVGTFLNGEDWLPATNVNFDT+L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

BLAST of CSPI03G28610.1 vs. ExPASy TrEMBL
Match: A0A5A7UGG5 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00970 PE=3 SV=1)

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 557/576 (96.70%), Postives = 572/576 (99.31%), Query Frame = 0

Query: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60
           MDTVKSFKGYGKVDELEQQAFRQKTRRRLIII IS++LLIALL+GAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120
           STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180
           SDYTSNFNS TGDPKV+AAIKIC+SVFEDAIDTLNDT+SSMEVDRHSEKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMVNSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDALRDLNQTTVLQ+LQTAM NSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQELQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300
           IHRKLMGLPEWVSSGDR+LLQENNVTAHVTVSKDGKG YTTI+DAVAAVPKKSKERFIIH
Sbjct: 241 IHRKLMGLPEWVSSGDRKLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYN+SL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540
           RPWKEFSTTVIM+SEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 577
           YKPSLTDSEAG+FTVGTFLNGEDWLPATNVNFDT+L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

BLAST of CSPI03G28610.1 vs. ExPASy TrEMBL
Match: A0A6J1GVT9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457891 PE=3 SV=1)

HSP 1 Score: 1028.5 bits (2658), Expect = 1.1e-296
Identity = 507/576 (88.02%), Postives = 544/576 (94.44%), Query Frame = 0

Query: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60
           MD+VKSFKGYGKVDELE QAFRQKTRRRLII+ +S++LLIAL++GAVVGIV+HKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120
           STT+S PPTEL P ASLKTLCSVTQYPSSCQSSL+NSNTTDP+FLFKLSLRVATDS+SKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180
           SDY SN NS+T D KV+AAI IC SVF+DAIDTLNDTVSSMEVDRH EKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMVNSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDA+RDLNQT VL++LQ  M NSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300
           IHRKLM  PEW+S GDRRLLQENNVT H TVSKDG G +TTI++AVAAVPKKSKERFII+
Sbjct: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDG+T+TIVSG LNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVM GCSFDGYQDTLYAHSNRQFYR+CDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSL APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540
           RPWKEFSTTVIM+SEIGGFL P+GWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRV+W G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540

Query: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 577
           Y+PSLTD+EA +FTV TFLNGE+WLPAT+V FDTSL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 576

BLAST of CSPI03G28610.1 vs. ExPASy TrEMBL
Match: A0A6J1IZN0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479904 PE=3 SV=1)

HSP 1 Score: 1025.0 bits (2649), Expect = 1.2e-295
Identity = 507/576 (88.02%), Postives = 543/576 (94.27%), Query Frame = 0

Query: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60
           MD+VKSFKGYGKVDELE QAFRQKTRRRLII+ +S++LLIAL++GAVVGIV+HKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120
           STT+S PPTEL P ASLKTLCSVTQYPSSCQSSL+NSNTTDP+FLFKLSLRVATDS+SKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180
           SDY SN NS+T D KV+AAI IC SVF+DAIDTLNDTVSSMEVDRH EKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICTSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMVNSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDA+RDLN T VL++LQ  M NSTEFTSNSLAIVTKILGLLA+FNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNHTAVLENLQAVMANSTEFTSNSLAIVTKILGLLANFNIP 240

Query: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300
           IHRKLM  PEWVS GDRRLLQENNVTAH TVSKDG  +YTTI++AVAAVPKKSKERFII+
Sbjct: 241 IHRKLMAFPEWVSPGDRRLLQENNVTAHATVSKDGTEEYTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDGRT+TIVSG LNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGRTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVM GCSFDGYQDTLYAHSNRQFYR+CDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480
           GNAA VFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSL APTYLG
Sbjct: 421 GNAAAVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540
           RPWKEFSTTVIM+SEIGGFL PVGWKEWVSGQDPPSSIFYGEYQNSGPGSNV+KRV+W G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVEKRVKWTG 540

Query: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 577
           Y+PSLTD+EA +FTV TFLNGEDWLPAT+V FD+SL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEDWLPATSVKFDSSL 576

BLAST of CSPI03G28610.1 vs. NCBI nr
Match: XP_004144445.1 (pectinesterase 3 [Cucumis sativus] >KGN58475.1 hypothetical protein Csa_001265 [Cucumis sativus])

HSP 1 Score: 1142.1 bits (2953), Expect = 0.0e+00
Identity = 575/576 (99.83%), Postives = 575/576 (99.83%), Query Frame = 0

Query: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60
           MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60

Query: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120
           STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180
           SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMVNSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDALRDLNQTTVLQDLQTAM NSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300
           IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480

Query: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540
           RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG
Sbjct: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 577
           YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL
Sbjct: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 576

BLAST of CSPI03G28610.1 vs. NCBI nr
Match: XP_008460354.1 (PREDICTED: pectinesterase 3 [Cucumis melo])

HSP 1 Score: 1118.6 bits (2892), Expect = 0.0e+00
Identity = 559/576 (97.05%), Postives = 572/576 (99.31%), Query Frame = 0

Query: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60
           MDTVKSFKGYGKVDELEQQAFRQKTRRRLIII IS++LLIALL+GAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120
           STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180
           SDYTSNFNS TGDPKV+AAIKIC+SVFEDAIDTLNDT+SSMEVDRHSEKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMVNSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDALRDLNQTTVLQDLQTAM NSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300
           IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKG YTTI+DAVAAVPKKSKERFIIH
Sbjct: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYN+SL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540
           RPWKEFSTTVIM+SEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 577
           YKPSLTDSEAG+FTVGTFLNGEDWLPATNVNFDT+L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

BLAST of CSPI03G28610.1 vs. NCBI nr
Match: KAA0053577.1 (pectinesterase 3 [Cucumis melo var. makuwa] >TYK19167.1 pectinesterase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 1115.9 bits (2885), Expect = 0.0e+00
Identity = 557/576 (96.70%), Postives = 572/576 (99.31%), Query Frame = 0

Query: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60
           MDTVKSFKGYGKVDELEQQAFRQKTRRRLIII IS++LLIALL+GAVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIISISVVLLIALLIGAVVGIVLHKRNSSSS 60

Query: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120
           STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL
Sbjct: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180
           SDYTSNFNS TGDPKV+AAIKIC+SVFEDAIDTLNDT+SSMEVDRHSEKFLSPSRIEDLK
Sbjct: 121 SDYTSNFNSNTGDPKVKAAIKICQSVFEDAIDTLNDTISSMEVDRHSEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMVNSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDALRDLNQTTVLQ+LQTAM NSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQELQTAMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300
           IHRKLMGLPEWVSSGDR+LLQENNVTAHVTVSKDGKG YTTI+DAVAAVPKKSKERFIIH
Sbjct: 241 IHRKLMGLPEWVSSGDRKLLQENNVTAHVTVSKDGKGDYTTIKDAVAAVPKKSKERFIIH 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYN+SL+APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNNSLSAPTYLG 480

Query: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540
           RPWKEFSTTVIM+SEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540

Query: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 577
           YKPSLTDSEAG+FTVGTFLNGEDWLPATNVNFDT+L
Sbjct: 541 YKPSLTDSEAGKFTVGTFLNGEDWLPATNVNFDTTL 576

BLAST of CSPI03G28610.1 vs. NCBI nr
Match: XP_038877946.1 (pectinesterase 3 [Benincasa hispida])

HSP 1 Score: 1071.2 bits (2769), Expect = 3.1e-309
Identity = 532/576 (92.36%), Postives = 561/576 (97.40%), Query Frame = 0

Query: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60
           MDTVKSFKGYGKVDELEQQAFRQKTRRRLII+ +S++LLIAL++ AVVGIV+HKRNSSSS
Sbjct: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIILLVSIVLLIALVIAAVVGIVIHKRNSSSS 60

Query: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120
           S T+SPPPTELTP ASLKTLCSVTQYPSSCQSSL+NSNTTDPVFLFKLSLRVATDSLSKL
Sbjct: 61  SATSSPPPTELTPLASLKTLCSVTQYPSSCQSSLENSNTTDPVFLFKLSLRVATDSLSKL 120

Query: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180
           SDYTSN NS T DPK++AAI+ICRSVFEDAIDTLNDTVSSMEVDRH+EKFLSPSRI+DLK
Sbjct: 121 SDYTSNLNSNTEDPKLKAAIEICRSVFEDAIDTLNDTVSSMEVDRHNEKFLSPSRIDDLK 180

Query: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMVNSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDALRDLNQTTVLQ+LQTAM NSTEFTSNSLAIVTKIL LLADF+IP
Sbjct: 181 TWLSTTITDQETCLDALRDLNQTTVLQNLQTAMANSTEFTSNSLAIVTKILRLLADFDIP 240

Query: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300
           IHRKLMG P+WVSSGDRRLLQENNVTAHVTVSKDGKG YTTI+DAVAAVPKKSK+RFII+
Sbjct: 241 IHRKLMGFPDWVSSGDRRLLQENNVTAHVTVSKDGKGNYTTIKDAVAAVPKKSKQRFIIY 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDGRTKTIVSG LNFIDGTPTFSTATFAVAGKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGRTKTIVSGRLNFIDGTPTFSTATFAVAGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSV+SGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVISGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYND+L APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDNLAAPTYLG 480

Query: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540
           RPWKEFSTTVIM+SEIGGFL PVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRV+WAG
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWAG 540

Query: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 577
           Y+PSLTD+EAG+FTVGTFLNGEDWLPAT+V FDTSL
Sbjct: 541 YRPSLTDAEAGKFTVGTFLNGEDWLPATSVKFDTSL 576

BLAST of CSPI03G28610.1 vs. NCBI nr
Match: XP_022956093.1 (pectinesterase 3-like [Cucurbita moschata] >KAG6581793.1 Pectinesterase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1028.5 bits (2658), Expect = 2.3e-296
Identity = 507/576 (88.02%), Postives = 544/576 (94.44%), Query Frame = 0

Query: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60
           MD+VKSFKGYGKVDELE QAFRQKTRRRLII+ +S++LLIAL++GAVVGIV+HKRNSSSS
Sbjct: 1   MDSVKSFKGYGKVDELEHQAFRQKTRRRLIILLLSIVLLIALVIGAVVGIVIHKRNSSSS 60

Query: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKL 120
           STT+S PPTEL P ASLKTLCSVTQYPSSCQSSL+NSNTTDP+FLFKLSLRVATDS+SKL
Sbjct: 61  STTSSTPPTELPPAASLKTLCSVTQYPSSCQSSLENSNTTDPIFLFKLSLRVATDSISKL 120

Query: 121 SDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLK 180
           SDY SN NS+T D KV+AAI IC SVF+DAIDTLNDTVSSMEVDRH EKFLSPSRIEDLK
Sbjct: 121 SDYASNLNSSTSDVKVKAAIGICSSVFKDAIDTLNDTVSSMEVDRHGEKFLSPSRIEDLK 180

Query: 181 TWLSTTITDQETCLDALRDLNQTTVLQDLQTAMVNSTEFTSNSLAIVTKILGLLADFNIP 240
           TWLSTTITDQETCLDA+RDLNQT VL++LQ  M NSTEFTSNSLAIVTKILGLLADFNIP
Sbjct: 181 TWLSTTITDQETCLDAIRDLNQTAVLENLQAVMANSTEFTSNSLAIVTKILGLLADFNIP 240

Query: 241 IHRKLMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIH 300
           IHRKLM  PEW+S GDRRLLQENNVT H TVSKDG G +TTI++AVAAVPKKSKERFII+
Sbjct: 241 IHRKLMAFPEWLSPGDRRLLQENNVTVHATVSKDGTGDFTTIKEAVAAVPKKSKERFIIY 300

Query: 301 VKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKD 360
           VKEG+YEENVILDKSKWNVMMYGDG+T+TIVSG LNFIDGTPT++TATFAV GKGFIGKD
Sbjct: 301 VKEGLYEENVILDKSKWNVMMYGDGKTRTIVSGRLNFIDGTPTYATATFAVQGKGFIGKD 360

Query: 361 MGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIF 420
           MGFINTAGPAKHQAVAFRSGSDLSVM GCSFDGYQDTLYAHSNRQFYR+CDITGTIDFIF
Sbjct: 361 MGFINTAGPAKHQAVAFRSGSDLSVMYGCSFDGYQDTLYAHSNRQFYRNCDITGTIDFIF 420

Query: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAPTYLG 480
           GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSL APTYLG
Sbjct: 421 GNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLTAPTYLG 480

Query: 481 RPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAG 540
           RPWKEFSTTVIM+SEIGGFL P+GWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRV+W G
Sbjct: 481 RPWKEFSTTVIMQSEIGGFLQPIGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVKWTG 540

Query: 541 YKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 577
           Y+PSLTD+EA +FTV TFLNGE+WLPAT+V FDTSL
Sbjct: 541 YRPSLTDAEAAKFTVRTFLNGEEWLPATSVKFDTSL 576

BLAST of CSPI03G28610.1 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 692.2 bits (1785), Expect = 3.6e-199
Identity = 356/590 (60.34%), Postives = 443/590 (75.08%), Query Frame = 0

Query: 1   MDTVKSFKGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSS 60
           MD+V SFKGYGKVDE +  A ++KTR+RL+++ IS+++LIA+++ AVV  VVHK  + S 
Sbjct: 1   MDSVNSFKGYGKVDEAQDLALKKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNES- 60

Query: 61  STTTSPPPTELTPPASLKTLCSVTQYPSSCQSS---LQNSNTTDPVFLFKLSLRVATDSL 120
             T SPPP ELTP  SLK +CSVT++P SC SS   L +SNTTDP  LFKLSL+V  D L
Sbjct: 61  --TPSPPP-ELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDEL 120

Query: 121 SKLSDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIE 180
             +SD     +  T D ++++A+++C  + EDA+D LNDTVS+++ D   +K LS S+IE
Sbjct: 121 DSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAID-DEEKKKTLSSSKIE 180

Query: 181 DLKTWLSTTITDQETCLDALRDLNQ-------TTVLQDLQTAMVNSTEFTSNSLAIVTKI 240
           DLKTWLS T+TD ETC D+L +L Q       +T+ Q+L++AM  STEFTSNSLAIV+KI
Sbjct: 181 DLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKI 240

Query: 241 LGLLADFNIPIHRK----------LMGLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYT 300
           L  L+D  IPIHR+           +   +W     RRLLQ   +   VTV+ DG G   
Sbjct: 241 LSALSDLGIPIHRRRRLMSHHHQQSVDFEKWAR---RRLLQTAGLKPDVTVAGDGTGDVL 300

Query: 301 TIQDAVAAVPKKSKERFIIHVKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDG 360
           T+ +AVA VPKKS + F+I+VK G Y ENV++DKSKWNVM+YGDG+ KTI+SG  NF+DG
Sbjct: 301 TVNEAVAKVPKKSLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDG 360

Query: 361 TPTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYA 420
           TPT+ TATFA+ GKGFI KD+G INTAG AKHQAVAFRSGSD SV   CSFDG+QDTLY 
Sbjct: 361 TPTYETATFAIQGKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYP 420

Query: 421 HSNRQFYRDCDITGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGIS 480
           HSNRQFYRDCD+TGTIDFIFG+AAVVFQ C I PRQPL NQFNTITAQGKKD NQ+SG+S
Sbjct: 421 HSNRQFYRDCDVTGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMS 480

Query: 481 IQKCTFSAYNDSLNAPTYLGRPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFY 540
           IQ+CT SA N ++ APTYLGRPWKEFSTTVIM + IG  + P GW  WVSG DPP+SI Y
Sbjct: 481 IQRCTISA-NGNVIAPTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVY 540

Query: 541 GEYQNSGPGSNVDKRVRWAGYKPSLTDSEAGRFTVGTFLNGEDWLPATNV 571
           GEY+N+GPGS+V +RV+WAGYKP ++D+EA +FTV T L+G DW+PAT V
Sbjct: 541 GEYKNTGPGSDVTQRVKWAGYKPVMSDAEAAKFTVATLLHGADWIPATGV 581

BLAST of CSPI03G28610.1 vs. TAIR 10
Match: AT3G14300.1 (pectinesterase family protein )

HSP 1 Score: 555.4 bits (1430), Expect = 5.2e-158
Identity = 294/528 (55.68%), Postives = 370/528 (70.08%), Query Frame = 0

Query: 52  VHKRNS-SSSSTTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQ----NSNTTDPVFLF 111
           +H+R+   S     SP P   TP + L+T+C+VT YP+SC SS+     +  TTDP  LF
Sbjct: 436 IHERHGVQSPRLRKSPHP---TPSSVLRTVCNVTNYPASCISSISKLPLSKTTTDPKVLF 495

Query: 112 KLSLRVATDSLSKLSDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSM-EVDR 171
           +LSL+V  D L+ +          T D  +++A+ +C  VF+ A+D++NDT+SS+ EV  
Sbjct: 496 RLSLQVTFDELNSIVGLPKKLAEETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVIS 555

Query: 172 HSEKFLSPSRIEDLKTWLSTTITDQETCLDALRDLN-QTTVLQDLQTAMVNSTEFTSNSL 231
             +K L+ S I DL TWLS+ +TD  TC D L + N  + + Q L++AMVNSTEFTSNSL
Sbjct: 556 GGKKNLNSSTIGDLITWLSSAVTDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSL 615

Query: 232 AIVTKILGLLADFNIPIH-RKLM---GLPEWVSSGDRRLLQENNVTAHVTVSKDGKGQYT 291
           AIV ++L   +   IP+  R+L+     P WV  G RRLLQ  N+T HVTV+ DG G   
Sbjct: 616 AIVAQVLKKPSKSRIPVQGRRLLNSNSFPNWVRPGVRRLLQAKNLTPHVTVAADGSGDVR 675

Query: 292 TIQDAVAAVPKKSKERFIIHVKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDG 351
           T+ +AV  VPKK K  F+I+VK G Y ENV++ K KWNV +YGDGR KTI+SG  N +DG
Sbjct: 676 TVNEAVWRVPKKGKTMFVIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDG 735

Query: 352 TPTFSTATFAVAGKGFIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYA 411
             TF+T+TFA  GKGF+ KDMG INTAGP KHQAVAFRS SD SV   CSFDGYQDTLY 
Sbjct: 736 VRTFNTSTFATEGKGFMMKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYT 795

Query: 412 HSNRQFYRDCDITGTIDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGIS 471
           HSNRQ+YR+CD+TGT+DFIFG   VVFQ C+IRPRQPLPNQFNTITA+G ++ NQN+GIS
Sbjct: 796 HSNRQYYRNCDVTGTVDFIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGIS 855

Query: 472 IQKCTFSAYNDSLNAPTYLGRPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQD-PPSSIF 531
           I +CT S  N ++ A TYLGRPWK FS TVIM+S IG F+NP GW  W S  D PP +IF
Sbjct: 856 IHQCTISP-NGNVTATTYLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIF 915

Query: 532 YGEYQNSGPGSNVDKRVRWAGYKPSLTDSEAGRFTVGTFLNGED-WLP 567
           Y EY+NSGPGS++ KRV+WAGYKP  +D EA RFTV  FL G+D W+P
Sbjct: 916 YREYKNSGPGSDLSKRVKWAGYKPISSDDEAARFTVKYFLRGDDNWIP 959

HSP 2 Score: 200.7 bits (509), Expect = 3.3e-51
Identity = 123/253 (48.62%), Postives = 169/253 (66.80%), Query Frame = 0

Query: 1   MDTVKSF-KGYGKVDELEQQAFRQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSS 60
           MDTVKS  KGYGKVDE +  A ++KTR+RL  I IS+ +L+A+++ + V I +H R  +S
Sbjct: 1   MDTVKSINKGYGKVDETQDLALKRKTRKRLYQIGISVAVLVAIIISSTVTIAIHSRKGNS 60

Query: 61  SSTTTSPPPTELTPPASLKTLCSVTQYPSSCQSSLQN---SNTTDPVFLFKLSLRVATDS 120
              T S  P ELTP ASLKT+CSVT YP SC SS+     SNTTDP  +F+LSL+V  D 
Sbjct: 61  PHPTPSSVP-ELTPAASLKTVCSVTNYPVSCFSSISKLPLSNTTDPEVIFRLSLQVVIDE 120

Query: 121 LSKLSDYTSNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRI 180
           L+ + +        T D  +++A+ +C  + + AID +N+TVS+MEV    +K L+ + I
Sbjct: 121 LNSIVELPKKLAEETDDEGLKSALSVCEHLLDLAIDRVNETVSAMEV-VDGKKILNAATI 180

Query: 181 EDLKTWLSTTITDQETCLDALRDLNQT--TVLQDLQTAMVNSTEFTSNSLAIVTKILGLL 240
           +DL TWLS  +T   TCLDAL +++ T   +   L++ MVNSTEFTSNSLAIV KIL  +
Sbjct: 181 DDLLTWLSAAVTYHGTCLDALDEISHTNSAIPLKLKSGMVNSTEFTSNSLAIVAKILSTI 240

Query: 241 ADFNIPIH-RKLM 247
           +DF IPIH R+L+
Sbjct: 241 SDFGIPIHGRRLL 251

BLAST of CSPI03G28610.1 vs. TAIR 10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 487.6 bits (1254), Expect = 1.3e-137
Identity = 277/579 (47.84%), Postives = 376/579 (64.94%), Query Frame = 0

Query: 10  YGKVDELEQQAFR--QKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSSSTTTSPP 69
           YG++DE EQ      +KT++R+ II IS ++L+ ++VGAVVG    + NS    T  +  
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTA-RDNSKKPPTENNGE 63

Query: 70  PTELTPPASLKTLCSVTQYPSSCQSSL---QNSNTTDPVFLFKLSLRVATDSLSKLSDYT 129
           P  +    S+K LC VT +   C  +L    N++ + P  LFK +++V    LSK+ D  
Sbjct: 64  PISV----SVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLDGF 123

Query: 130 SNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLKTWLS 189
           SN      D    AA+  C  +   A+D LN+T++S           S    +DL+TWLS
Sbjct: 124 SNGEHM--DNATSAAMGACVELIGLAVDQLNETMTS-----------SLKNFDDLRTWLS 183

Query: 190 TTITDQETCLDALRDLNQTTVLQDLQTAMVNSTEFTSNSLAIVTKILGLLADFNIPIHRK 249
           +  T QETC+DAL + N+ ++    +  + NSTE TSN+LAI+T  LG +AD      R+
Sbjct: 184 SVGTYQETCMDALVEANKPSLTTFGENHLKNSTEMTSNALAIIT-WLGKIADTVKFRRRR 243

Query: 250 LMG-------LPEWVSSGDRRLLQENNV--TAHVTVSKDGKGQYTTIQDAVAAVPKKSKE 309
           L+        + +      RRLL+  ++   A + V+KDG G+Y TI +A+A V +K+++
Sbjct: 244 LLETGNAKVVVADLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEK 303

Query: 310 RFIIHVKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKG 369
             II+VK+G+Y ENV ++K+KWNV+M GDG++KTIVS  LNFIDGTPTF TATFAV GKG
Sbjct: 304 PTIIYVKKGVYLENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKG 363

Query: 370 FIGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGT 429
           F+ +DMGFINTAGPAKHQAVA    +DLSV   C+ D +QDT+YAH+ RQFYRDC I GT
Sbjct: 364 FMARDMGFINTAGPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGT 423

Query: 430 IDFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNA 489
           +DFIFGNAAVVFQ C I PR+P+  Q NTITAQG+KD NQN+GISI  CT    ++  + 
Sbjct: 424 VDFIFGNAAVVFQKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDI 483

Query: 490 PTYLGRPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKR 549
            T+LGRPWK+FSTTVIM+S +  F+NP GW  W +G   P +IFY EY NSGPG++   R
Sbjct: 484 QTFLGRPWKDFSTTVIMKSFMDKFINPKGWLPW-TGDTAPDTIFYAEYLNSGPGASTKNR 543

Query: 550 VRWAGYKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDT 575
           V+W G K SLT  EA +FTV  F++G +WLPAT V F++
Sbjct: 544 VKWQGLKTSLTKKEANKFTVKPFIDGNNWLPATKVPFNS 562

BLAST of CSPI03G28610.1 vs. TAIR 10
Match: AT3G10710.1 (root hair specific 12 )

HSP 1 Score: 461.1 bits (1185), Expect = 1.3e-129
Identity = 258/580 (44.48%), Postives = 372/580 (64.14%), Query Frame = 0

Query: 10  YGKVDELEQQAF--RQKTRRRLIIIFISLLLLIALLVGAVVGIVVHKRNSSSSSTTTSPP 69
           YGKVDE E      R+KTR+ + II +SL++L  +++GAV G + HK++  +  T  +  
Sbjct: 5   YGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNGD 64

Query: 70  PTELTPPASLKTLCSVTQYPSSCQSSL---QNSNTTDPVFLFKLSLRVATDSLSKLSDYT 129
              +    S+K +C VT +   C  +L    N+++ +P  LF+ ++++   +++++S   
Sbjct: 65  SISV----SVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKI---TIAEVSKAI 124

Query: 130 SNFNSTTGDPKVEAAIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLKTWLS 189
           + F+S+ GD K    +  C  + +  ID LN+T++S      +     P  ++DL+TWLS
Sbjct: 125 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSS----NGDVTVPELVDDLRTWLS 184

Query: 190 TTITDQETCLDALRDLNQTTVLQDL----QTAMVNSTEFTSNSLAIVTKILGLLADFNIP 249
           +  T Q TC++        T+  D+    ++ + NSTE TSN+LAI+T  LG +AD +  
Sbjct: 185 SAGTYQRTCVE--------TLAPDMRPFGESHLKNSTELTSNALAIIT-WLGKIAD-SFK 244

Query: 250 IHRKLMGLP--EWVSSGDRRLLQENNV--TAHVTVSKDGKGQYTTIQDAVAAVPKKSKER 309
           + R+L+     E      RRLLQ  ++   A + V+KDG G+Y TI+ A+  VP+KS++R
Sbjct: 245 LRRRLLTTADVEVDFHAGRRLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKR 304

Query: 310 FIIHVKEGIYEENVILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGF 369
            II+VK+G+Y ENV ++K  WNV++ GDG +K+IVSG LN IDGTPTF TATFAV GKGF
Sbjct: 305 TIIYVKKGVYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGF 364

Query: 370 IGKDMGFINTAGPAKHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTI 429
           + +DMGFINTAGP+KHQAVA    +DL+    C+ + YQDTLY H+ RQFYR+C I GT+
Sbjct: 365 MARDMGFINTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTV 424

Query: 430 DFIFGNAAVVFQNCNIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSLNAP 489
           DFIFGN+A V Q+C I PR+P+  Q NTITAQG+ D N N+GISI +C  S   D  +  
Sbjct: 425 DFIFGNSASVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVM 484

Query: 490 TYLGRPWKEFSTTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRV 549
           T+LGRPWK FSTTVIM S + GF++  GW  W +G   P +IFYGEY+N+GPG++   RV
Sbjct: 485 TFLGRPWKNFSTTVIMDSYLHGFIDRKGWLPW-TGDSAPDTIFYGEYKNTGPGASTKNRV 544

Query: 550 RWAGYKPSLTDSEAGRFTVGTFLNGEDWLPATNVNFDTSL 577
           +W G +  L+  EA RFTV  F++G  WLPAT V F + L
Sbjct: 545 KWKGLR-FLSTKEANRFTVKPFIDGGRWLPATKVPFRSGL 561

BLAST of CSPI03G28610.1 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 397.9 bits (1021), Expect = 1.4e-110
Identity = 213/510 (41.76%), Postives = 300/510 (58.82%), Query Frame = 0

Query: 79  TLCSVTQYPSSCQSSLQNSNTTDPVFLFKLSLRVATDSLSKLSDYTSNFNSTTGDPKVEA 138
           +LCS  +   S    +Q      P   F  + +   D+++K     S F+   G  +V  
Sbjct: 20  SLCSAHKEAFSSTDLVQMECLRVPPLEFAEAAKTVVDAITKAVAIVSKFDKKAGKSRVSN 79

Query: 139 AIKICRSVFEDAIDTLNDTVSSMEVDRHSEKFLSPSRIEDLKTWLSTTITDQETCLDALR 198
           AI  C  + + A + L+  +S+ +     +         DL+TW+S  +++Q+TCLD   
Sbjct: 80  AIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVG-SDLRTWISAALSNQDTCLDGFE 139

Query: 199 DLNQTTVLQDLQTAMVNSTEFTSNSLAIVT-------KILGLLADFNIPIHRKLMGLPEW 258
             N   +++ +    ++    T  +L  +        K   + A      H      P W
Sbjct: 140 GTN--GIIKKIVAGGLSKVGTTVRNLLTMVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSW 199

Query: 259 VSSGDRRLLQENNVT-AHVTVSKDGKGQYTTIQDAVAAVPKKSKERFIIHVKEGIYEENV 318
           V  GDR+LLQ +N+T A   V+ DG G +TTI DAV A P  S +R++IHVK G+Y ENV
Sbjct: 200 VKPGDRKLLQTDNITVADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENV 259

Query: 319 ILDKSKWNVMMYGDGRTKTIVSGHLNFIDGTPTFSTATFAVAGKGFIGKDMGFINTAGPA 378
            + K KWN+MM GDG   T+++G+ +FIDG  TF +ATFAV+G+GFI +D+ F NTAGP 
Sbjct: 260 EIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPE 319

Query: 379 KHQAVAFRSGSDLSVMSGCSFDGYQDTLYAHSNRQFYRDCDITGTIDFIFGNAAVVFQNC 438
           KHQAVA RS +DL V   C+  GYQDTLYAHS RQF+R+C ITGT+DFIFG+A  VFQ+C
Sbjct: 320 KHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSC 379

Query: 439 NIRPRQPLPNQFNTITAQGKKDINQNSGISIQKCTFSAYNDSL----NAPTYLGRPWKEF 498
            I+ +Q LPNQ N+ITAQG+KD N+ +G +IQ    +A  D L       TYLGRPWK +
Sbjct: 380 QIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLY 439

Query: 499 STTVIMRSEIGGFLNPVGWKEWVSGQDPPSSIFYGEYQNSGPGSNVDKRVRWAGYKPSLT 558
           S TV M++ +   +NPVGW EW +G     +++YGEY NSGPG+++D+RV+W GY    T
Sbjct: 440 SRTVFMQNYMSDAINPVGWLEW-NGNFALDTLYYGEYMNSGPGASLDRRVKWPGYHVLNT 499

Query: 559 DSEAGRFTVGTFLNGEDWLPATNVNFDTSL 577
            +EA  FTV   + G  WLP+T + F   L
Sbjct: 500 SAEANNFTVSQLIQGNLWLPSTGITFIAGL 525