CSPI03G23380.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI03G23380.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	Chr3: 20258351 .. 20259590 (-)
Sequence length	921
RNA-Seq Expression	CSPI03G23380.1
Synteny	CSPI03G23380.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTTTATCAAAGTAACACCTTCTGCTGAACAGAATTTGAATGAACCTTCTACCAGCCATCAAGTAGAGACTCATTCAAATTTGAAAGACTTAAACCCTAACAGCAGTGATCAACTTCCAACAAAAGCTCTACCTAGCTTTCGTCCTCAAGAAAAAGTAGAAGAAAATGCAAAAGACTTGACAAATTATAGCCTAATAAGAGATAGAGGGAAAAGAACCATTAGGCCTCCTTCTAGATTTGCAAGAGCAGTTTGCATAGCTAACTCCTCTACAGAGACTATAGAAGATGAGCCTTACAGTTATGAAGATGCTCTAAACAACAATTGATGGAAAGAAGCCATGAATGATGAACTTATTTCACTTTACAAGAATGATACTTGGGAACTAGTAGAAAAGCCACATGGAAAACGTATCATTCCTTGTAAATGGGTCTTCAAGAAGAAGATGATTGGTGATTTAAATGACAAAGTAAAATTTAAGGCAAGACTAGTAGCTAAAGGATTCAAGCAAAAGGAGAGTGTAGACTATAATGAGATTTTTTTCCCCTATTGTGAAGCACACTTCTATAAGAATCCTACTGGCTATTGTAGCATGTGAAAATCTTGAGCTGGAGTAGTTAGATGTTACTACCGCTTTCCTACATGGAAGCTTAGAAGAAGAAATCTTCATGGAGCAGCCTAAGGGTTTTGAAGTAAAAGGCAAAAAGGAGCTGGTGTGTAAACTAAATAGGTCTTTATATGGCCTAAAACAGTCTCCAAGGTGTTGGTAAAAACGTTTTGATGACTATATCAGCCTGATAGGCTTTATTAGAAGTCTATATGACCCATGTGTCTACTACAAGCAGCTCACTGAAGGTGATTACATCTATCTTTTACTCTACGTTGATGATATGTTACTAGCAAAGAGAGATCCAATCAAGTTAAAAGAAATCAAAGCTCAACTAAGTATTGAATTTGATAAGAAAGACTCAAGGGCAACTAAGAAGATCCTAAGAATTGAAATCTTGAGAGATAGAAATAACAATGAGTTATCTCTAACTCATAAGACCTACACCAACAACGTACTATGTAAATTCAACATGGCTAGCTCAAAGGCAGTTTCTACACCTTTAGCACAACACATTAAAATATCAGCAAGAGATTCACCTAAAGATCCGACTGACAAACAAGCAATGAGCTATGTACCATATTCTAATGCTATAATTCAAGTCTTGTCAGCTGAGACATGGGAAACCTAG

mRNA sequence

ATGGAGTTTATCAAAGTAACACCTTCTGCTGAACAGAATTTGAATGAACCTTCTACCAGCCATCAAGTAGAGACTCATTCAAATTTGAAAGACTTAAACCCTAACAGCAGTGATCAACTTCCAACAAAAGCTCTACCTAGCTTTCGTCCTCAAGAAAAAGTAGAAGAAAATGCAAAAGACTTGACAAATTATAGCCTAATAAGAGATAGAGGGAAAAGAACCATTAGGCCTCCTTCTAGATTTGCAAGAGCAGTTTGCATAGCTAACTCCTCTACAGAGACTATAGAAGATGAGCCTTACATAGAAAAGCCACATGGAAAACGTATCATTCCTTGTAAATGGGTCTTCAAGAAGAAGATGATTGGTGATTTAAATGACAAAGTAAAATTTAAGGCAAGACTAGTAGCTAAAGGATTCAAGCAAAAGGAGAGTTTAGATGTTACTACCGCTTTCCTACATGGAAGCTTAGAAGAAGAAATCTTCATGGAGCAGCCTAAGGGTTTTGAAGTAAAAGGCAAAAAGGAGCTGCTCACTGAAGGTGATTACATCTATCTTTTACTCTACGTTGATGATATGTTACTAGCAAAGAGAGATCCAATCAAGTTAAAAGAAATCAAAGCTCAACTAAGTATTGAATTTGATAAGAAAGACTCAAGGGCAACTAAGAAGATCCTAAGAATTGAAATCTTGAGAGATAGAAATAACAATGAGTTATCTCTAACTCATAAGACCTACACCAACAACGTACTATGTAAATTCAACATGGCTAGCTCAAAGGCAGTTTCTACACCTTTAGCACAACACATTAAAATATCAGCAAGAGATTCACCTAAAGATCCGACTGACAAACAAGCAATGAGCTATGTACCATATTCTAATGCTATAATTCAAGTCTTGTCAGCTGAGACATGGGAAACCTAG

Coding sequence (CDS)

ATGGAGTTTATCAAAGTAACACCTTCTGCTGAACAGAATTTGAATGAACCTTCTACCAGCCATCAAGTAGAGACTCATTCAAATTTGAAAGACTTAAACCCTAACAGCAGTGATCAACTTCCAACAAAAGCTCTACCTAGCTTTCGTCCTCAAGAAAAAGTAGAAGAAAATGCAAAAGACTTGACAAATTATAGCCTAATAAGAGATAGAGGGAAAAGAACCATTAGGCCTCCTTCTAGATTTGCAAGAGCAGTTTGCATAGCTAACTCCTCTACAGAGACTATAGAAGATGAGCCTTACATAGAAAAGCCACATGGAAAACGTATCATTCCTTGTAAATGGGTCTTCAAGAAGAAGATGATTGGTGATTTAAATGACAAAGTAAAATTTAAGGCAAGACTAGTAGCTAAAGGATTCAAGCAAAAGGAGAGTTTAGATGTTACTACCGCTTTCCTACATGGAAGCTTAGAAGAAGAAATCTTCATGGAGCAGCCTAAGGGTTTTGAAGTAAAAGGCAAAAAGGAGCTGCTCACTGAAGGTGATTACATCTATCTTTTACTCTACGTTGATGATATGTTACTAGCAAAGAGAGATCCAATCAAGTTAAAAGAAATCAAAGCTCAACTAAGTATTGAATTTGATAAGAAAGACTCAAGGGCAACTAAGAAGATCCTAAGAATTGAAATCTTGAGAGATAGAAATAACAATGAGTTATCTCTAACTCATAAGACCTACACCAACAACGTACTATGTAAATTCAACATGGCTAGCTCAAAGGCAGTTTCTACACCTTTAGCACAACACATTAAAATATCAGCAAGAGATTCACCTAAAGATCCGACTGACAAACAAGCAATGAGCTATGTACCATATTCTAATGCTATAATTCAAGTCTTGTCAGCTGAGACATGGGAAACCTAG

Protein sequence

MEFIKVTPSAEQNLNEPSTSHQVETHSNLKDLNPNSSDQLPTKALPSFRPQEKVEENAKDLTNYSLIRDRGKRTIRPPSRFARAVCIANSSTETIEDEPYIEKPHGKRIIPCKWVFKKKMIGDLNDKVKFKARLVAKGFKQKESLDVTTAFLHGSLEEEIFMEQPKGFEVKGKKELLTEGDYIYLLLYVDDMLLAKRDPIKLKEIKAQLSIEFDKKDSRATKKILRIEILRDRNNNELSLTHKTYTNNVLCKFNMASSKAVSTPLAQHIKISARDSPKDPTDKQAMSYVPYSNAIIQVLSAETWET*

Homology

[Close]

BLAST of CSPI03G23380.1 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 2.0e-36
Identity = 102/277 (36.82%), Postives = 141/277 (50.90%), Query Frame = 0


Query: 101  IEKPHGKRIIPCKWVFKKKMIGDLNDKVKFKARLVAKGFKQK------------------ 160
            +E P GKR + CKWVFK K  GD    V++KARLV KGF+QK                  
Sbjct: 846  VELPKGKRPLKCKWVFKLKKDGDCK-LVRYKARLVVKGFEQKKGIDFDEIFSPVVKMTSI 905

Query: 161  --------------ESLDVTTAFLHGSLEEEIFMEQPKGFEVKGKKELL----------- 220
                          E LDV TAFLHG LEEEI+MEQP+GFEV GKK ++           
Sbjct: 906  RTILSLAASLDLEVEQLDVKTAFLHGDLEEEIYMEQPEGFEVAGKKHMVCKLNKSLYGLK 965

Query: 221  ---------------------------------TEGDYIYLLLYVDDMLLAKRDPIKLKE 280
                                             +E ++I LLLYVDDML+  +D   + +
Sbjct: 966  QAPRQWYMKFDSFMKSQTYLKTYSDPCVYFKRFSENNFIILLLYVDDMLIVGKDKGLIAK 1025

Query: 281  IKAQLSIEFDKKDSRATKKILRIEILRDRNNNELSLTHKTYTNNVLCKFNMASSKAVSTP 302
            +K  LS  FD KD    ++IL ++I+R+R + +L L+ + Y   VL +FNM ++K VSTP
Sbjct: 1026 LKGDLSKSFDMKDLGPAQQILGMKIVRERTSRKLWLSQEKYIERVLERFNMKNAKPVSTP 1085

[Close]

BLAST of CSPI03G23380.1 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 82.4 bits (202), Expect = 9.7e-15
Identity = 94/360 (26.11%), Postives = 150/360 (41.67%), Query Frame = 0


Query: 17   PSTSHQVETHSNLKDL---NPNSSD----------QLPTKALPSFRPQE----KVEENAK 76
            P+ S + ET  +LK++   NP  +D          +L TK   S+  ++    KV  NA 
Sbjct: 825  PNESRESETAEHLKEIGIDNPTKNDGIEIINRRSERLKTKPQISYNEEDNSLNKVVLNAH 884

Query: 77   DLTN-----YSLIRDRGKRTIRPPSRFARAVCIANSSTETIEDEPYIEKPHGKRIIPCKW 136
             + N     +  I+ R  +     S +  A+    ++ +        ++P  K I+  +W
Sbjct: 885  TIFNDVPNSFDEIQYRDDK-----SSWEEAINTELNAHKINNTWTITKRPENKNIVDSRW 944

Query: 137  VFKKKMIGDLNDKVKFKARLVAKGFKQK-------------------------------- 196
            VF  K   +L + +++KARLVA+GF QK                                
Sbjct: 945  VFSVK-YNELGNPIRYKARLVARGFTQKYQIDYEETFAPVARISSFRFILSLVIQYNLKV 1004

Query: 197  ESLDVTTAFLHGSLEEEIFMEQPKG-------------------------FEV--KGKKE 256
              +DV TAFL+G+L+EEI+M  P+G                         FEV  +  KE
Sbjct: 1005 HQMDVKTAFLNGTLKEEIYMRLPQGISCNSDNVCKLNKAIYGLKQAARCWFEVFEQALKE 1064

Query: 257  -------------LLTEG---DYIYLLLYVDDMLLAKRDPIKLKEIKAQLSIEFDKKDSR 280
                         +L +G   + IY+LLYVDD+++A  D  ++   K  L  +F   D  
Sbjct: 1065 CEFVNSSVDRCIYILDKGNINENIYVLLYVDDVVIATGDMTRMNNFKRYLMEKFRMTDLN 1124

[Close]

BLAST of CSPI03G23380.1 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.7e-14
Identity = 96/380 (25.26%), Postives = 149/380 (39.21%), Query Frame = 0


Query: 7    TPSAEQNLNEPSTSHQVETHSNLKDLNPNSSDQLPTKALPSFRPQEK-VEENAKDLTNYS 66
            +P +  ++  PSTS        + + N  SS    T  LP   P    ++ NA+   N  
Sbjct: 859  SPISSPHIPTPSTS--------ISEPNSPSSSSTSTPPLPPVLPAPPIIQVNAQAPVNTH 918

Query: 67   LIRDRGKRTIRPPS-RFARAVCIANSS-----TETIEDEPYIE----------------- 126
             +  R K  IR P+ +++ A  +A +S      + ++D+ + +                 
Sbjct: 919  SMATRAKDGIRKPNQKYSYATSLAANSEPRTAIQAMKDDRWRQAMGSEINAQIGNHTWDL 978

Query: 127  ---KPHGKRIIPCKWVFKKKMIGDLNDKVKFKARLVAKGFKQK----------------- 186
                P    I+ C+W+F KK   D     ++KARLVAKG+ Q+                 
Sbjct: 979  VPPPPPSVTIVGCRWIFTKKFNSD-GSLNRYKARLVAKGYNQRPGLDYAETFSPVIKSTS 1038

Query: 187  ---------------ESLDVTTAFLHGSLEEEIFMEQPKGFEVKGKKE------------ 246
                             LDV  AFL G+L +E++M QP GF  K + +            
Sbjct: 1039 IRIVLGVAVDRSWPIRQLDVNNAFLQGTLTDEVYMSQPPGFVDKDRPDYVCRLRKAIYGL 1098

Query: 247  --------------LLTEG-----------------DYIYLLLYVDDMLLAKRDPIKLKE 283
                          LLT G                   IY+L+YVDD+L+   D + LK 
Sbjct: 1099 KQAPRAWYVELRTYLLTVGFVNSISDTSLFVLQRGRSIIYMLVYVDDILITGNDTVLLKH 1158

[Close]

BLAST of CSPI03G23380.1 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.0e-12
Identity = 71/256 (27.73%), Postives = 101/256 (39.45%), Query Frame = 0


Query: 104  PHGKRIIPCKWVFKKKMIGDLNDKVKFKARLVAKGFKQK--------------------- 163
            P    I+ C+W+F KK   D     ++KARLVAKG+ Q+                     
Sbjct: 992  PSHVTIVGCRWIFTKKYNSD-GSLNRYKARLVAKGYNQRPGLDYAETFSPVIKSTSIRIV 1051

Query: 164  -----------ESLDVTTAFLHGSLEEEIFMEQPKGF----------------------- 223
                         LDV  AFL G+L ++++M QP GF                       
Sbjct: 1052 LGVAVDRSWPIRQLDVNNAFLQGTLTDDVYMSQPPGFIDKDRPNYVCKLRKALYGLKQAP 1111

Query: 224  ---EVKGKKELLTEG-----------------DYIYLLLYVDDMLLAKRDPIKLKEIKAQ 283
                V+ +  LLT G                   +Y+L+YVDD+L+   DP  L      
Sbjct: 1112 RAWYVELRNYLLTIGFVNSVSDTSLFVLQRGKSIVYMLVYVDDILITGNDPTLLHNTLDN 1171

[Close]

BLAST of CSPI03G23380.1 vs. ExPASy TrEMBL
Match: A0A5A7U6R2 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold333G00930 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 1.2e-103
Identity = 224/333 (67.27%), Postives = 237/333 (71.17%), Query Frame = 0


Query: 1   MEFIKVTPSAEQNLNEPSTSHQVETHSNLKDLNPNSSDQLPTKALPSFRPQ-EKVEENAK 60
           ME IKV P AE+NLNEPSTSHQVE HSNLK+LNP+SSDQLPT+ALPSF  Q E+ EENA+
Sbjct: 564 MESIKVIP-AEKNLNEPSTSHQVEIHSNLKNLNPSSSDQLPTEALPSFYSQEEEEEENAE 623

Query: 61  DLTNYSLIRDRGKRTIRPPSRFARAVCIANSSTETIEDEP------YIEKPHGKRIIPCK 120
           DLTNYSL RDRG+RTI+PPSRFARA CI NSS ETIED+P        +KPHGK IIPCK
Sbjct: 624 DLTNYSLTRDRGRRTIKPPSRFARADCIVNSSIETIEDKPDSYEDALYKKPHGKIIIPCK 683

Query: 121 WVFKKKMIGDLNDKVKFKARLVAKGFKQK------------------------------- 180
           WVFKKKMIGDLNDKVKFKARLVAKGFKQK                               
Sbjct: 684 WVFKKKMIGDLNDKVKFKARLVAKGFKQKEGVDYNEIFPPVVKHTSIRILLAIVACENLE 743

Query: 181 -ESLDVTTAFLHGSLEEEIFMEQPKGFEVKGKKELLTEGDYIYLLLYVDDMLLAKRDPIK 240
            E LDVTTAFLHGSLEEEIFMEQP G                             RD  K
Sbjct: 744 LEQLDVTTAFLHGSLEEEIFMEQPMG-----------------------------RDLTK 803

Query: 241 LKEIKAQLSIEFDKKDSRATKKILRIEILRDRNNNELSLTHKTYTNNVLCKFNMASSKAV 295
           LKEIKAQLS EFD KD  A KKIL IEILRDRNNNELSLT KTYTN VLC+FNMA+SK V
Sbjct: 804 LKEIKAQLSAEFDMKDLGAAKKILGIEILRDRNNNELSLTQKTYTNKVLCRFNMANSKVV 863

[Close]

BLAST of CSPI03G23380.1 vs. ExPASy TrEMBL
Match: A0A5D3BAM8 (Retrotransposon protein, putative, Ty1-copia subclass OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold767G00260 PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.4e-101
Identity = 216/328 (65.85%), Postives = 228/328 (69.51%), Query Frame = 0


Query: 1   MEFIKVTPSAEQNLNEPSTSHQVETHSNLKDLNPNSSDQLPTKALPSFRPQ-EKVEENAK 60
           M+ IKV PSAE NLNEPSTSHQVE HSNLKD NP+SSDQLPTKA PSF PQ E+ EENA+
Sbjct: 289 MDSIKVIPSAEHNLNEPSTSHQVEIHSNLKDSNPSSSDQLPTKAFPSFHPQEEEEEENAE 348

Query: 61  DLTNYSLIRDRGKRTIRPPSRFARAVCIANSSTETIEDEPY------------------- 120
           DLTNYSL RDRG++TIRPPSRFARA CIANSS ETIEDEPY                   
Sbjct: 349 DLTNYSLTRDRGRKTIRPPSRFARADCIANSSIETIEDEPYSYKDALCSRHNNQWKEAMN 408

Query: 121 --------------IEKPHGKRIIPCKWVFKKKMIGDLNDKVKFKARLVAKGFKQKESLD 180
                         +E PHGK IIPCKWVFKKKMIG LNDKVKFKARLVAKGF+QKE  D
Sbjct: 409 DELNSLYKNDTWELVETPHGKSIIPCKWVFKKKMIGYLNDKVKFKARLVAKGFEQKE--D 468

Query: 181 VTTAFLHGSLEEEIFMEQPKGFEVKGKKELLTEGDYIYLLLYVDDMLLAKRDPIKLKEIK 240
           VTTAFL GSLEEEI MEQPKGFEVKGK EL                              
Sbjct: 469 VTTAFLRGSLEEEILMEQPKGFEVKGKMEL------------------------------ 528

Query: 241 AQLSIEFDKKDSRATKKILRIEILRDRNNNELSLTHKTYTNNVLCKFNMASSKAVSTPLA 295
                     D  A KKIL IEILRDRNNNELSLT KTYTN VLC+FN+A+SK VSTPLA
Sbjct: 529 ----------DLGAAKKILGIEILRDRNNNELSLTQKTYTNKVLCRFNLANSKVVSTPLA 574

[Close]

BLAST of CSPI03G23380.1 vs. ExPASy TrEMBL
Match: A0A5A7SVP7 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold260G00170 PE=4 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 1.6e-100
Identity = 216/332 (65.06%), Postives = 234/332 (70.48%), Query Frame = 0


Query: 1   MEFIKVTPSAEQNLNEPSTSHQVETHSNLKDLNPNSSDQLPTKALPSFRPQ-EKVEENAK 60
           ME IKV PS EQNLNE STSHQVE HSNLKDLNP+SSDQLPT+ALPSF PQ E+ EENA+
Sbjct: 317 MESIKVIPSTEQNLNEASTSHQVEIHSNLKDLNPSSSDQLPTEALPSFHPQEEEEEENAE 376

Query: 61  DLTNYSLIRDRGKRTIRPPSRFARAVCIANSSTETIEDEPY------------------- 120
           DLTNYSL RDRG+RTIRPPSRFARA CIANSSTETI+DEPY                   
Sbjct: 377 DLTNYSLTRDRGRRTIRPPSRFARADCIANSSTETIKDEPYSYEDALYSRHNNQWKEAMN 436

Query: 121 --------------IEKPHGKRIIPCKWVFKKKMIGDLNDK---VKFKARLVAKGFKQKE 180
                         +EKPHGK IIPCKWVFKKKMIG+LNDK   ++    +VA    + E
Sbjct: 437 AELNSLYKKDTLKLVEKPHGKSIIPCKWVFKKKMIGELNDKHTSIRILLAIVACENLELE 496

Query: 181 SLDVTTAFLHGSLEEEIFMEQPKGFEVKGKKELLTEGDYIYLLLYVDDMLLAKRDPIKLK 240
            LDVTTAFLHGSLE+EIFMEQPKGFEVKGKKEL              D+L      +   
Sbjct: 497 QLDVTTAFLHGSLEKEIFMEQPKGFEVKGKKEL--------------DLLEVYMTHVSTT 556

Query: 241 EIKAQLSIEFDKKDSRATKKILRIEILRDRNNNELSLTHKTYTNNVLCKFNMASSKAVST 296
            I          KD  ATKKIL IEILRDRNNNELSLT KTYTN VLC+FNMA+SK V T
Sbjct: 557 RISL--------KDLGATKKILGIEILRDRNNNELSLTQKTYTNKVLCRFNMANSKVVFT 616

[Close]

BLAST of CSPI03G23380.1 vs. ExPASy TrEMBL
Match: A0A5D3CYI6 (Retrotransposon protein, putative, Ty1-copia subclass OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G002160 PE=4 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 1.1e-90
Identity = 204/304 (67.11%), Postives = 217/304 (71.38%), Query Frame = 0


Query: 1   MEFIKVTPSAEQNLNEPSTSHQVETHSNLKDLNPNSSDQLPTKALPSFRP-QEKVEENAK 60
           ME IKV PS EQNLNE ST+HQVE HSNLKD NP+ SDQLPT+ LPSF P +E+ +EN +
Sbjct: 1   MEPIKVIPSTEQNLNESSTNHQVEIHSNLKDSNPSISDQLPTETLPSFHPLEEEEKENTE 60

Query: 61  DLTNYSLIRDRGKRTIRPPSRFARAVCIANSSTETIEDEPY------------------- 120
           DLT+YSL  DR +RTIRP SRFARA CIANSS ETIEDEPY                   
Sbjct: 61  DLTSYSLTGDRRRRTIRPLSRFARANCIANSSIETIEDEPYSYEDALYSRHSNQWKEAMN 120

Query: 121 --------------IEKPHGKRIIPCKWVFKKKMIGDLNDKVKFKARLVAKGFKQKESLD 180
                         +EKPHGK IIPCKWVFKKKMIGDLN+KVKFKARLVAKGFKQK   D
Sbjct: 121 DELNSLYKNDTWEQVEKPHGKIIIPCKWVFKKKMIGDLNNKVKFKARLVAKGFKQKG--D 180

Query: 181 VTTAFLHGSLEEEIFMEQP--KGFE-------VKGKKELLTEGDYIYLLLYVDDMLLAKR 240
           VTTAFLHGSLEEEIFMEQP   GF        V  KK  LTEGDYIYLLLYVDDMLLA R
Sbjct: 181 VTTAFLHGSLEEEIFMEQPCLIGFIRSLYDPCVYYKK--LTEGDYIYLLLYVDDMLLAGR 240

Query: 241 DPIKLKEIKAQLSIEFDKKDSRATKKILRIEILRDRNNNELSLTHKTYTNNVLCKFNMAS 262
           DPIKLKEIKAQLS+EFD KD  A KKILRIEILRDRNNNELSLT KTYTN         S
Sbjct: 241 DPIKLKEIKAQLSVEFDMKDLGAAKKILRIEILRDRNNNELSLTQKTYTNKARSSSQFKS 300

[Close]

BLAST of CSPI03G23380.1 vs. ExPASy TrEMBL
Match: A0A5A7TWF0 (Putative retroelement pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold243G00700 PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 8.1e-81
Identity = 193/374 (51.60%), Postives = 213/374 (56.95%), Query Frame = 0


Query: 1   MEFIKVTPSAEQNLNEPSTSHQVETHSNLKDLNPNSSDQLPTKALPSFRPQ-EKVEENAK 60
           ME+IKVTPS EQN+NEP TSHQVE HSNLKD NP+SSDQLPT+ALPSF PQ E+ EEN K
Sbjct: 411 MEYIKVTPSVEQNMNEPCTSHQVEIHSNLKDSNPSSSDQLPTEALPSFHPQEEEEEENTK 470

Query: 61  DLTNYSLIRDRGKRTIRPPSRFARAVCIANSSTETIEDEPY------------------- 120
           DLTNYSL +D+G+RTI+PP RFARA CI NSST++IED+ Y                   
Sbjct: 471 DLTNYSLTKDKGRRTIKPP-RFARADCITNSSTDSIEDDHYSYKDALTSRHNNQWKDVGV 530

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 531 QREADRREAGRMREGHMDASGFLFASAISDLSLVRKSWVVTKEAMNDELNSLYKNDTWKL 590

Query: 181 IEKPHGKRIIPCKWVFKKKMIGDLNDKVKFKARLVAKGFKQKESLDVTTAFLHGSLEEEI 240
           ++KPHGK +IPCKWVFKKKMI       K    LVA    + E LDVTTAFLHGSLEEEI
Sbjct: 591 VKKPHGKSMIPCKWVFKKKMIAHF---YKNPTGLVACENLELEQLDVTTAFLHGSLEEEI 650

Query: 241 FMEQPKGFEVKGKKELLTEGDYIYLLLYVDDMLLAKRDPIKLKEIKAQLSIEFDKKDSRA 295
           FMEQPK FEVKGKKEL                                        D  A
Sbjct: 651 FMEQPKDFEVKGKKEL----------------------------------------DLGA 710

[Close]

BLAST of CSPI03G23380.1 vs. NCBI nr
Match: KAA0051442.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa] >TYK22211.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 386.3 bits (991), Expect = 2.4e-103
Identity = 224/333 (67.27%), Postives = 237/333 (71.17%), Query Frame = 0


Query: 1   MEFIKVTPSAEQNLNEPSTSHQVETHSNLKDLNPNSSDQLPTKALPSFRPQ-EKVEENAK 60
           ME IKV P AE+NLNEPSTSHQVE HSNLK+LNP+SSDQLPT+ALPSF  Q E+ EENA+
Sbjct: 564 MESIKVIP-AEKNLNEPSTSHQVEIHSNLKNLNPSSSDQLPTEALPSFYSQEEEEEENAE 623

Query: 61  DLTNYSLIRDRGKRTIRPPSRFARAVCIANSSTETIEDEP------YIEKPHGKRIIPCK 120
           DLTNYSL RDRG+RTI+PPSRFARA CI NSS ETIED+P        +KPHGK IIPCK
Sbjct: 624 DLTNYSLTRDRGRRTIKPPSRFARADCIVNSSIETIEDKPDSYEDALYKKPHGKIIIPCK 683

Query: 121 WVFKKKMIGDLNDKVKFKARLVAKGFKQK------------------------------- 180
           WVFKKKMIGDLNDKVKFKARLVAKGFKQK                               
Sbjct: 684 WVFKKKMIGDLNDKVKFKARLVAKGFKQKEGVDYNEIFPPVVKHTSIRILLAIVACENLE 743

Query: 181 -ESLDVTTAFLHGSLEEEIFMEQPKGFEVKGKKELLTEGDYIYLLLYVDDMLLAKRDPIK 240
            E LDVTTAFLHGSLEEEIFMEQP G                             RD  K
Sbjct: 744 LEQLDVTTAFLHGSLEEEIFMEQPMG-----------------------------RDLTK 803

Query: 241 LKEIKAQLSIEFDKKDSRATKKILRIEILRDRNNNELSLTHKTYTNNVLCKFNMASSKAV 295
           LKEIKAQLS EFD KD  A KKIL IEILRDRNNNELSLT KTYTN VLC+FNMA+SK V
Sbjct: 804 LKEIKAQLSAEFDMKDLGAAKKILGIEILRDRNNNELSLTQKTYTNKVLCRFNMANSKVV 863

[Close]

BLAST of CSPI03G23380.1 vs. NCBI nr
Match: KAA0039651.1 (retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa] >TYJ95535.1 retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa])

HSP 1 Score: 379.4 bits (973), Expect = 2.9e-101
Identity = 216/328 (65.85%), Postives = 228/328 (69.51%), Query Frame = 0


Query: 1   MEFIKVTPSAEQNLNEPSTSHQVETHSNLKDLNPNSSDQLPTKALPSFRPQ-EKVEENAK 60
           M+ IKV PSAE NLNEPSTSHQVE HSNLKD NP+SSDQLPTKA PSF PQ E+ EENA+
Sbjct: 289 MDSIKVIPSAEHNLNEPSTSHQVEIHSNLKDSNPSSSDQLPTKAFPSFHPQEEEEEENAE 348

Query: 61  DLTNYSLIRDRGKRTIRPPSRFARAVCIANSSTETIEDEPY------------------- 120
           DLTNYSL RDRG++TIRPPSRFARA CIANSS ETIEDEPY                   
Sbjct: 349 DLTNYSLTRDRGRKTIRPPSRFARADCIANSSIETIEDEPYSYKDALCSRHNNQWKEAMN 408

Query: 121 --------------IEKPHGKRIIPCKWVFKKKMIGDLNDKVKFKARLVAKGFKQKESLD 180
                         +E PHGK IIPCKWVFKKKMIG LNDKVKFKARLVAKGF+QKE  D
Sbjct: 409 DELNSLYKNDTWELVETPHGKSIIPCKWVFKKKMIGYLNDKVKFKARLVAKGFEQKE--D 468

Query: 181 VTTAFLHGSLEEEIFMEQPKGFEVKGKKELLTEGDYIYLLLYVDDMLLAKRDPIKLKEIK 240
           VTTAFL GSLEEEI MEQPKGFEVKGK EL                              
Sbjct: 469 VTTAFLRGSLEEEILMEQPKGFEVKGKMEL------------------------------ 528

Query: 241 AQLSIEFDKKDSRATKKILRIEILRDRNNNELSLTHKTYTNNVLCKFNMASSKAVSTPLA 295
                     D  A KKIL IEILRDRNNNELSLT KTYTN VLC+FN+A+SK VSTPLA
Sbjct: 529 ----------DLGAAKKILGIEILRDRNNNELSLTQKTYTNKVLCRFNLANSKVVSTPLA 574

[Close]

BLAST of CSPI03G23380.1 vs. NCBI nr
Match: KAA0033501.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa] >TYK26828.1 Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 375.9 bits (964), Expect = 3.2e-100
Identity = 216/332 (65.06%), Postives = 234/332 (70.48%), Query Frame = 0


Query: 1   MEFIKVTPSAEQNLNEPSTSHQVETHSNLKDLNPNSSDQLPTKALPSFRPQ-EKVEENAK 60
           ME IKV PS EQNLNE STSHQVE HSNLKDLNP+SSDQLPT+ALPSF PQ E+ EENA+
Sbjct: 317 MESIKVIPSTEQNLNEASTSHQVEIHSNLKDLNPSSSDQLPTEALPSFHPQEEEEEENAE 376

Query: 61  DLTNYSLIRDRGKRTIRPPSRFARAVCIANSSTETIEDEPY------------------- 120
           DLTNYSL RDRG+RTIRPPSRFARA CIANSSTETI+DEPY                   
Sbjct: 377 DLTNYSLTRDRGRRTIRPPSRFARADCIANSSTETIKDEPYSYEDALYSRHNNQWKEAMN 436

Query: 121 --------------IEKPHGKRIIPCKWVFKKKMIGDLNDK---VKFKARLVAKGFKQKE 180
                         +EKPHGK IIPCKWVFKKKMIG+LNDK   ++    +VA    + E
Sbjct: 437 AELNSLYKKDTLKLVEKPHGKSIIPCKWVFKKKMIGELNDKHTSIRILLAIVACENLELE 496

Query: 181 SLDVTTAFLHGSLEEEIFMEQPKGFEVKGKKELLTEGDYIYLLLYVDDMLLAKRDPIKLK 240
            LDVTTAFLHGSLE+EIFMEQPKGFEVKGKKEL              D+L      +   
Sbjct: 497 QLDVTTAFLHGSLEKEIFMEQPKGFEVKGKKEL--------------DLLEVYMTHVSTT 556

Query: 241 EIKAQLSIEFDKKDSRATKKILRIEILRDRNNNELSLTHKTYTNNVLCKFNMASSKAVST 296
            I          KD  ATKKIL IEILRDRNNNELSLT KTYTN VLC+FNMA+SK V T
Sbjct: 557 RISL--------KDLGATKKILGIEILRDRNNNELSLTQKTYTNKVLCRFNMANSKVVFT 616

[Close]

BLAST of CSPI03G23380.1 vs. NCBI nr
Match: KAA0062923.1 (retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa] >TYK16420.1 retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa])

HSP 1 Score: 343.2 bits (879), Expect = 2.3e-90
Identity = 204/304 (67.11%), Postives = 217/304 (71.38%), Query Frame = 0


Query: 1   MEFIKVTPSAEQNLNEPSTSHQVETHSNLKDLNPNSSDQLPTKALPSFRP-QEKVEENAK 60
           ME IKV PS EQNLNE ST+HQVE HSNLKD NP+ SDQLPT+ LPSF P +E+ +EN +
Sbjct: 1   MEPIKVIPSTEQNLNESSTNHQVEIHSNLKDSNPSISDQLPTETLPSFHPLEEEEKENTE 60

Query: 61  DLTNYSLIRDRGKRTIRPPSRFARAVCIANSSTETIEDEPY------------------- 120
           DLT+YSL  DR +RTIRP SRFARA CIANSS ETIEDEPY                   
Sbjct: 61  DLTSYSLTGDRRRRTIRPLSRFARANCIANSSIETIEDEPYSYEDALYSRHSNQWKEAMN 120

Query: 121 --------------IEKPHGKRIIPCKWVFKKKMIGDLNDKVKFKARLVAKGFKQKESLD 180
                         +EKPHGK IIPCKWVFKKKMIGDLN+KVKFKARLVAKGFKQK   D
Sbjct: 121 DELNSLYKNDTWEQVEKPHGKIIIPCKWVFKKKMIGDLNNKVKFKARLVAKGFKQKG--D 180

Query: 181 VTTAFLHGSLEEEIFMEQP--KGFE-------VKGKKELLTEGDYIYLLLYVDDMLLAKR 240
           VTTAFLHGSLEEEIFMEQP   GF        V  KK  LTEGDYIYLLLYVDDMLLA R
Sbjct: 181 VTTAFLHGSLEEEIFMEQPCLIGFIRSLYDPCVYYKK--LTEGDYIYLLLYVDDMLLAGR 240

Query: 241 DPIKLKEIKAQLSIEFDKKDSRATKKILRIEILRDRNNNELSLTHKTYTNNVLCKFNMAS 262
           DPIKLKEIKAQLS+EFD KD  A KKILRIEILRDRNNNELSLT KTYTN         S
Sbjct: 241 DPIKLKEIKAQLSVEFDMKDLGAAKKILRIEILRDRNNNELSLTQKTYTNKARSSSQFKS 300

[Close]

BLAST of CSPI03G23380.1 vs. NCBI nr
Match: TYK28825.1 (putative retroelement pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 310.5 bits (794), Expect = 1.7e-80
Identity = 193/374 (51.60%), Postives = 213/374 (56.95%), Query Frame = 0


Query: 1   MEFIKVTPSAEQNLNEPSTSHQVETHSNLKDLNPNSSDQLPTKALPSFRPQ-EKVEENAK 60
           ME+IKVTPS EQN+NEP TSHQVE HSNLKD NP+SSDQLPT+ALPSF PQ E+ EEN K
Sbjct: 335 MEYIKVTPSVEQNMNEPCTSHQVEIHSNLKDSNPSSSDQLPTEALPSFHPQEEEEEENTK 394

Query: 61  DLTNYSLIRDRGKRTIRPPSRFARAVCIANSSTETIEDEPY------------------- 120
           DLTNYSL +D+G+RTI+PP RFARA CI NSST++IED+ Y                   
Sbjct: 395 DLTNYSLTKDKGRRTIKPP-RFARADCITNSSTDSIEDDHYSYKDALTSRHNNQWKDVGV 454

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 455 QREADRREAGRMREGHMDASGFLFASAISDLSLVRKSWVVTKEAMNDELNSLYKNDTWKL 514

Query: 181 IEKPHGKRIIPCKWVFKKKMIGDLNDKVKFKARLVAKGFKQKESLDVTTAFLHGSLEEEI 240
           ++KPHGK +IPCKWVFKKKMI       K    LVA    + E LDVTTAFLHGSLEEEI
Sbjct: 515 VKKPHGKSMIPCKWVFKKKMIAHF---YKNPTGLVACENLELEQLDVTTAFLHGSLEEEI 574

Query: 241 FMEQPKGFEVKGKKELLTEGDYIYLLLYVDDMLLAKRDPIKLKEIKAQLSIEFDKKDSRA 295
           FMEQPK FEVKGKKEL                                        D  A
Sbjct: 575 FMEQPKDFEVKGKKEL----------------------------------------DLGA 634

[Close]

BLAST of CSPI03G23380.1 vs. TAIR 10
Match: AT4G23160.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 8 )

HSP 1 Score: 60.1 bits (144), Expect = 3.7e-09
Identity = 60/249 (24.10%), Postives = 99/249 (39.76%), Query Frame = 0


Query: 104 PHGKRIIPCKWVFKKKMIGDLNDKVKFKARLVAKGFKQKE-------------------- 163
           P  K+ I CKWV+K K   D     ++KARLVAKG+ Q+E                    
Sbjct: 121 PPNKKPIGCKWVYKIKYNSD-GTIERYKARLVAKGYTQQEGIDFIETFSPVCKLTSVKLI 180

Query: 164 ------------SLDVTTAFLHGSLEEEIFMEQPKGF------------------EVKGK 223
                        LD++ AFL+G L+EEI+M+ P G+                   + G 
Sbjct: 181 LAISAIYNFTLHQLDISNAFLNGDLDEEIYMKLPPGYAARQGDSLPPNAVCYLKKSIYGL 240

Query: 224 KE------------------LLTEGDYIY-----------LLLYVDDMLLAKRDPIKLKE 274
           K+                  + +  D+ Y           +L+YVDD+++   +   + E
Sbjct: 241 KQASRQWFLKFSVTLIGFGFVQSHSDHTYFLKITATLFLCVLVYVDDIIICSNNDAAVDE 300

The following BLAST results are available for this feature:

BLAST of CSPI03G23380.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10978	2.0e-36	36.82	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]
P04146	9.7e-15	26.11	Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3	[more]
Q9ZT94	1.7e-14	25.26	Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O...	[more]
Q94HW2	2.0e-12	27.73	Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O...	[more]

BLAST of CSPI03G23380.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U6R2	1.2e-103	67.27	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var....	[more]
A0A5D3BAM8	1.4e-101	65.85	Retrotransposon protein, putative, Ty1-copia subclass OS=Cucumis melo var. makuw...	[more]
A0A5A7SVP7	1.6e-100	65.06	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var....	[more]
A0A5D3CYI6	1.1e-90	67.11	Retrotransposon protein, putative, Ty1-copia subclass OS=Cucumis melo var. makuw...	[more]
A0A5A7TWF0	8.1e-81	51.60	Putative retroelement pol polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]

BLAST of CSPI03G23380.1 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0051442.1	2.4e-103	67.27	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m...	[more]
KAA0039651.1	2.9e-101	65.85	retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa]...	[more]
KAA0033501.1	3.2e-100	65.06	Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m...	[more]
KAA0062923.1	2.3e-90	67.11	retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa]...	[more]
TYK28825.1	1.7e-80	51.60	putative retroelement pol polyprotein [Cucumis melo var. makuwa]	[more]

BLAST of CSPI03G23380.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23160.1	3.7e-09	24.10	cysteine-rich RLK (RECEPTOR-like protein kinase) 8	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013103	Reverse transcriptase, RNA-dependent DNA polymerase	PFAM	PF07727	RVT_2	coord: 183..265 e-value: 4.2E-11 score: 42.9 coord: 101..142 e-value: 1.0E-7 score: 31.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..55
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 8..44

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G23380	CSPI03G23380	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G23380.1.cds4	CSPI03G23380.1.cds4	CDS
CSPI03G23380.1.cds3	CSPI03G23380.1.cds3	CDS
CSPI03G23380.1.cds2	CSPI03G23380.1.cds2	CDS
CSPI03G23380.1.cds1	CSPI03G23380.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G23380.1	CSPI03G23380.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005488	binding