CSPI03G21520.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI03G21520.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	EF hand calcium-binding protein family
Location	Chr3: 17810960 .. 17811872 (-)
Sequence length	913
RNA-Seq Expression	CSPI03G21520.1
Synteny	CSPI03G21520.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAAAGGTAAAAAAACCTCTCTCTATTCAAATTTTAAATCCTCTTCACAATCAACACCATGGCCAAGAACAACTCCCTTTCAATTTCTACTCCAGGATCCTCCGCCTTAGGCTCCATGGACGAAATCATCCGAGTCTTCAACAAATTCGACAAGAACGGCGACGGCAAGATTTCCGTCACAGAACTCGCCGCTGCTCTCGGTGAACTCAGCGGCAACATCTCCACCGACGAGATCCACCGCATTATGTCCGAGATCGATAAGGACGGTGACGGTTTCATCGACCTCGATGAGTTCACCGACTTCACCTCCTCTTCCACTGGAGGAAACAAAGATCTGCAGGACGCTTTCGATCTCTACGATATTGATAAGAACGGCTTAATCTCTGCCAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCTGTTGATGTTGACGGTGATGGACATGTAAATTTCGAGGAATTCAAGAAGATGATGACGAGATCTTAGTGTTCTTCGCCAGTAGAGAGGTAGTTTCACAAATTTAATCAAATTTAGGCTTTAGAATTTCGAGACCAAAATCGAGTATTATCGGTTCATTCTTAGTGTGTACAGTCCATGAATCTATCCTTTCTTTCACGGTTTGTAACCGATTTAGGGCACCAAACGCCTCTACAATGAATTTCTCCTTTGATTTTAGATCTTAATCGGAGATCGGAAAAGGAAAAAAAAAGAACAAACAGATTATAAGTTCATATCATCTCCCTCTAATTGATTCTGATTTTCTCTTTCTGAAACGGACGGAAGAATCCGAAGTTGGAGGAAGTAAATTTTGTTGAATATTTGATTGGTTGATGCTATAGTTATTGAAGCAATTATTTGCATCGGTTAG

mRNA sequence

AAAAAAAGGTAAAAAAACCTCTCTCTATTCAAATTTTAAATCCTCTTCACAATCAACACCATGGCCAAGAACAACTCCCTTTCAATTTCTACTCCAGGATCCTCCGCCTTAGGCTCCATGGACGAAATCATCCGAGTCTTCAACAAATTCGACAAGAACGGCGACGGCAAGATTTCCGTCACAGAACTCGCCGCTGCTCTCGGTGAACTCAGCGGCAACATCTCCACCGACGAGATCCACCGCATTATGTCCGAGATCGATAAGGACGGTGACGGTTTCATCGACCTCGATGAGTTCACCGACTTCACCTCCTCTTCCACTGGAGGAAACAAAGATCTGCAGGACGCTTTCGATCTCTACGATATTGATAAGAACGGCTTAATCTCTGCCAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCTGTTGATGTTGACGGTGATGGACATGTAAATTTCGAGGAATTCAAGAAGATGATGACGAGATCTTAGTGTTCTTCGCCAGTAGAGAGGTAGTTTCACAAATTTAATCAAATTTAGGCTTTAGAATTTCGAGACCAAAATCGAGTATTATCGGTTCATTCTTAGTGTGTACAGTCCATGAATCTATCCTTTCTTTCACGGTTTGTAACCGATTTAGGGCACCAAACGCCTCTACAATGAATTTCTCCTTTGATTTTAGATCTTAATCGGAGATCGGAAAAGGAAAAAAAAAGAACAAACAGATTATAAGTTCATATCATCTCCCTCTAATTGATTCTGATTTTCTCTTTCTGAAACGGACGGAAGAATCCGAAGTTGGAGGAAGTAAATTTTGTTGAATATTTGATTGGTTGATGCTATAGTTATTGAAGCAATTATTTGCATCGGTTAG

Coding sequence (CDS)

ATGGCCAAGAACAACTCCCTTTCAATTTCTACTCCAGGATCCTCCGCCTTAGGCTCCATGGACGAAATCATCCGAGTCTTCAACAAATTCGACAAGAACGGCGACGGCAAGATTTCCGTCACAGAACTCGCCGCTGCTCTCGGTGAACTCAGCGGCAACATCTCCACCGACGAGATCCACCGCATTATGTCCGAGATCGATAAGGACGGTGACGGTTTCATCGACCTCGATGAGTTCACCGACTTCACCTCCTCTTCCACTGGAGGAAACAAAGATCTGCAGGACGCTTTCGATCTCTACGATATTGATAAGAACGGCTTAATCTCTGCCAAGGAGTTGCACTCTGTTCTCAAACGCCTTGGTGAAAAATGTAGCCTCAAAGATTGTTGCCGGATGATCAGTTCTGTTGATGTTGACGGTGATGGACATGTAAATTTCGAGGAATTCAAGAAGATGATGACGAGATCTTAG

Protein sequence

MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS*

Homology

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BLAST of CSPI03G21520.1 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 9.0e-41
Identity = 85/144 (59.03%), Postives = 106/144 (73.61%), Query Frame = 0


Query: 15  SALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFI 74
           + LGSM++I +VF +FDKN DGKIS+ EL   +G LS N S +E   +M E D DG+GFI
Sbjct: 8   NCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFI 67

Query: 75  DLDEFTDF-----TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDC 134
           DLDEF         SS+    +DL++AFDLYD+D+NG ISA ELHSV+K LGEKCS++DC
Sbjct: 68  DLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDC 127

Query: 135 CRMISSVDVDGDGHVNFEEFKKMM 154
            RMI+ VD DGDG V+FEEFKKMM
Sbjct: 128 QRMINKVDSDGDGCVDFEEFKKMM 151

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BLAST of CSPI03G21520.1 vs. ExPASy Swiss-Prot
Match: P25070 (Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2)

HSP 1 Score: 167.2 bits (422), Expect = 1.5e-40
Identity = 86/147 (58.50%), Postives = 105/147 (71.43%), Query Frame = 0


Query: 15  SALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFI 74
           S LGSMD+I +VF +FDKNGDGKISV EL   +  LS   S +E   +M + D DG+GFI
Sbjct: 10  SCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFI 69

Query: 75  DLDEFTDFTSSSTGGN-------KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLK 134
           DLDEF        GG         DL++AF+LYD+D NG ISAKELHSV+K LGEKCS++
Sbjct: 70  DLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQ 129

Query: 135 DCCRMISSVDVDGDGHVNFEEFKKMMT 155
           DC +MIS VD+DGDG VNF+EFKKMM+
Sbjct: 130 DCKKMISKVDIDGDGCVNFDEFKKMMS 156

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BLAST of CSPI03G21520.1 vs. ExPASy Swiss-Prot
Match: Q9LE22 (Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 1.9e-38
Identity = 78/146 (53.42%), Postives = 105/146 (71.92%), Query Frame = 0


Query: 11  TPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDG 70
           TP +  + + +E+ +VF++FD NGDGKISV EL      +  + +  E++R++ E+D D 
Sbjct: 12  TPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDR 71

Query: 71  DGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCC 130
           DG+I+LDEF+    SS+    +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC 
Sbjct: 72  DGYINLDEFSTLCRSSSSA-AEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCT 131

Query: 131 RMISSVDVDGDGHVNFEEFKKMMTRS 157
           RMI  VD DGDG+VNFEEF+KMMT S
Sbjct: 132 RMIGPVDADGDGNVNFEEFQKMMTSS 156

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BLAST of CSPI03G21520.1 vs. ExPASy Swiss-Prot
Match: Q9M7R0 (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.0e-36
Identity = 80/140 (57.14%), Postives = 98/140 (70.00%), Query Frame = 0


Query: 21  DEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFIDLDEFT 80
           +E+  VFN+FD NGDGKIS  ELA  L  L  N S +EI RIM EID D DGFI++ EF 
Sbjct: 19  NEVQGVFNRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKDGFINVQEFA 78

Query: 81  DFTSS------STGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRMIS 140
            F  +      S+GG  +L++AF+LYD D NGLIS+ ELH +L RLGE+ +  DC  MI 
Sbjct: 79  AFVKAETDPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLGERYAEHDCVEMIK 138

Query: 141 SVDVDGDGHVNFEEFKKMMT 155
           SVD DGDG+V+FEEFKKMMT
Sbjct: 139 SVDSDGDGYVSFEEFKKMMT 158

[Close]

BLAST of CSPI03G21520.1 vs. ExPASy Swiss-Prot
Match: Q9C9U8 (Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 PE=1 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 3.6e-34
Identity = 74/145 (51.03%), Postives = 103/145 (71.03%), Query Frame = 0


Query: 10  STPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKD 69
           ST  S+   +  E+ +VF+KFD NGDGKISV+EL      +  + + +E++R++ EID D
Sbjct: 8   STNKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDID 67

Query: 70  GDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDC 129
            DGFI+ +EF     SS+    ++++AFDLYD +KNGLIS+ E+H VL RLG  CS++DC
Sbjct: 68  CDGFINQEEFATICRSSSSA-VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDC 127

Query: 130 CRMISSVDVDGDGHVNFEEFKKMMT 155
            RMI  VD DGDG+VNFEEF+KMM+
Sbjct: 128 VRMIGHVDTDGDGNVNFEEFQKMMS 151

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BLAST of CSPI03G21520.1 vs. ExPASy TrEMBL
Match: A0A0A0LCM1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G319280 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 9.2e-81
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 0


Query: 1   MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH 60
           MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH
Sbjct: 1   MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH 60

Query: 61  RIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 120
           RIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL
Sbjct: 61  RIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 120

Query: 121 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 156

[Close]

BLAST of CSPI03G21520.1 vs. ExPASy TrEMBL
Match: A0A5A7UHX3 (Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold451G001160 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.4e-76
Identity = 150/157 (95.54%), Postives = 153/157 (97.45%), Query Frame = 0


Query: 1   MAKNN-SLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEI 60
           MA NN SLSISTPGSS+LGSMDE+IRVFNKFDKNGDGKISV+EL AALGELSGNIST EI
Sbjct: 1   MANNNPSLSISTPGSSSLGSMDEVIRVFNKFDKNGDGKISVSELGAALGELSGNISTGEI 60

Query: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120
           HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR
Sbjct: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120

Query: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157

[Close]

BLAST of CSPI03G21520.1 vs. ExPASy TrEMBL
Match: A0A1S3C5W9 (probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103496821 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.4e-76
Identity = 150/157 (95.54%), Postives = 153/157 (97.45%), Query Frame = 0


Query: 1   MAKNN-SLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEI 60
           MA NN SLSISTPGSS+LGSMDE+IRVFNKFDKNGDGKISV+EL AALGELSGNIST EI
Sbjct: 1   MANNNPSLSISTPGSSSLGSMDEVIRVFNKFDKNGDGKISVSELGAALGELSGNISTGEI 60

Query: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120
           HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR
Sbjct: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120

Query: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157

[Close]

BLAST of CSPI03G21520.1 vs. ExPASy TrEMBL
Match: A0A6J1G9H5 (probable calcium-binding protein CML23 OS=Cucurbita moschata OX=3662 GN=LOC111452166 PE=4 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 8.6e-63
Identity = 129/160 (80.62%), Postives = 140/160 (87.50%), Query Frame = 0


Query: 1   MAKNNSLSISTPG-SSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEI 60
           MAK +  + S+P    ALGSMDE+I VF KFDKNGDGKIS+ ELAAALGEL G IS DEI
Sbjct: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEI 62

Query: 61  HRIMSEIDKDGDGFIDLDEFTDF---TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSV 120
            RIMSEIDKDG+GFIDLDEF +F   +SS+ GG+KDLQDAFDLYD+DKNGLISAKELHSV
Sbjct: 63  RRIMSEIDKDGNGFIDLDEFAEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

[Close]

BLAST of CSPI03G21520.1 vs. ExPASy TrEMBL
Match: A0A6J1CNU4 (probable calcium-binding protein CML23 OS=Momordica charantia OX=3673 GN=LOC111013180 PE=4 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 1.1e-62
Identity = 126/156 (80.77%), Postives = 137/156 (87.82%), Query Frame = 0


Query: 8   SISTPGSS----ALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIM 67
           S+S P S+    ALGSMDE+ +VFNKFDKNGDGKISV EL  ALGEL G +S+DE+ RIM
Sbjct: 7   SVSDPSSTNLGGALGSMDEVTKVFNKFDKNGDGKISVNELGDALGELGGKVSSDEVRRIM 66

Query: 68  SEIDKDGDGFIDLDEFTDF---TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 127
           +EIDKDGDGFIDLDEF +F    S++ GGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL
Sbjct: 67  AEIDKDGDGFIDLDEFAEFHQDVSATGGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 126

Query: 128 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 127 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

[Close]

BLAST of CSPI03G21520.1 vs. NCBI nr
Match: XP_004145834.1 (probable calcium-binding protein CML23 [Cucumis sativus])

HSP 1 Score: 309.3 bits (791), Expect = 1.9e-80
Identity = 156/156 (100.00%), Postives = 156/156 (100.00%), Query Frame = 0


Query: 1   MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH 60
           MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH
Sbjct: 1   MAKNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIH 60

Query: 61  RIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 120
           RIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL
Sbjct: 61  RIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRL 120

Query: 121 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 GEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 156

[Close]

BLAST of CSPI03G21520.1 vs. NCBI nr
Match: XP_008457051.1 (PREDICTED: probable calcium-binding protein CML27 [Cucumis melo] >KAA0053141.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa] >TYK01546.1 putative calcium-binding protein CML27 [Cucumis melo var. makuwa])

HSP 1 Score: 295.4 bits (755), Expect = 2.8e-76
Identity = 150/157 (95.54%), Postives = 153/157 (97.45%), Query Frame = 0


Query: 1   MAKNN-SLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEI 60
           MA NN SLSISTPGSS+LGSMDE+IRVFNKFDKNGDGKISV+EL AALGELSGNIST EI
Sbjct: 1   MANNNPSLSISTPGSSSLGSMDEVIRVFNKFDKNGDGKISVSELGAALGELSGNISTGEI 60

Query: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120
           HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR
Sbjct: 61  HRIMSEIDKDGDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 120

Query: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 121 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157

[Close]

BLAST of CSPI03G21520.1 vs. NCBI nr
Match: KAE8650604.1 (hypothetical protein Csa_010664 [Cucumis sativus])

HSP 1 Score: 287.7 bits (735), Expect = 5.9e-74
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 13  GSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDG 72
           GSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDG
Sbjct: 6   GSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDG 65

Query: 73  FIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRM 132
           FIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRM
Sbjct: 66  FIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCCRM 125

Query: 133 ISSVDVDGDGHVNFEEFKKMMTRS 157
           ISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 126 ISSVDVDGDGHVNFEEFKKMMTRS 149

[Close]

BLAST of CSPI03G21520.1 vs. NCBI nr
Match: XP_038879983.1 (probable calcium-binding protein CML23 [Benincasa hispida])

HSP 1 Score: 266.5 bits (680), Expect = 1.4e-67
Identity = 138/157 (87.90%), Postives = 143/157 (91.08%), Query Frame = 0


Query: 3   KNNSLSISTPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRI 62
           KNNSLSIS PGSS LGSMD++I+VFNKFDKNGDGKISV EL AAL EL G ISTDEI RI
Sbjct: 4   KNNSLSISNPGSS-LGSMDDVIKVFNKFDKNGDGKISVNELGAALSELGGKISTDEIRRI 63

Query: 63  MSEIDKDGDGFIDLDEFTDF---TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKR 122
           MSEIDKDGDGFIDLDEF +F    SSS+G NKDLQDAFDLYDIDKNGLISAKELHSVLKR
Sbjct: 64  MSEIDKDGDGFIDLDEFAEFHQDASSSSGCNKDLQDAFDLYDIDKNGLISAKELHSVLKR 123

Query: 123 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 124 LGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 159

[Close]

BLAST of CSPI03G21520.1 vs. NCBI nr
Match: XP_022948508.1 (probable calcium-binding protein CML23 [Cucurbita moschata] >XP_023524703.1 probable calcium-binding protein CML23 [Cucurbita pepo subsp. pepo] >KAG6607012.1 putative calcium-binding protein CML23, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036709.1 putative calcium-binding protein CML23, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 249.6 bits (636), Expect = 1.8e-62
Identity = 129/160 (80.62%), Postives = 140/160 (87.50%), Query Frame = 0


Query: 1   MAKNNSLSISTPG-SSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEI 60
           MAK +  + S+P    ALGSMDE+I VF KFDKNGDGKIS+ ELAAALGEL G IS DEI
Sbjct: 3   MAKTSVSNPSSPNPGGALGSMDEVIMVFKKFDKNGDGKISIKELAAALGELGGKISDDEI 62

Query: 61  HRIMSEIDKDGDGFIDLDEFTDF---TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSV 120
            RIMSEIDKDG+GFIDLDEF +F   +SS+ GG+KDLQDAFDLYD+DKNGLISAKELHSV
Sbjct: 63  RRIMSEIDKDGNGFIDLDEFAEFHQNSSSTGGGSKDLQDAFDLYDVDKNGLISAKELHSV 122

Query: 121 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 157
           LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS
Sbjct: 123 LKRLGEKCSLKDCCRMISSVDVDGDGHVNFEEFKKMMTRS 162

[Close]

BLAST of CSPI03G21520.1 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 167.9 bits (424), Expect = 6.4e-42
Identity = 85/144 (59.03%), Postives = 106/144 (73.61%), Query Frame = 0


Query: 15  SALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFI 74
           + LGSM++I +VF +FDKN DGKIS+ EL   +G LS N S +E   +M E D DG+GFI
Sbjct: 8   NCLGSMEDIKKVFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFI 67

Query: 75  DLDEFTDF-----TSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDC 134
           DLDEF         SS+    +DL++AFDLYD+D+NG ISA ELHSV+K LGEKCS++DC
Sbjct: 68  DLDEFVALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLGEKCSIQDC 127

Query: 135 CRMISSVDVDGDGHVNFEEFKKMM 154
            RMI+ VD DGDG V+FEEFKKMM
Sbjct: 128 QRMINKVDSDGDGCVDFEEFKKMM 151

[Close]

BLAST of CSPI03G21520.1 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 167.2 bits (422), Expect = 1.1e-41
Identity = 86/147 (58.50%), Postives = 105/147 (71.43%), Query Frame = 0


Query: 15  SALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDGDGFI 74
           S LGSMD+I +VF +FDKNGDGKISV EL   +  LS   S +E   +M + D DG+GFI
Sbjct: 10  SCLGSMDDIKKVFQRFDKNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFI 69

Query: 75  DLDEFTDFTSSSTGGN-------KDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLK 134
           DLDEF        GG         DL++AF+LYD+D NG ISAKELHSV+K LGEKCS++
Sbjct: 70  DLDEFVALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLGEKCSVQ 129

Query: 135 DCCRMISSVDVDGDGHVNFEEFKKMMT 155
           DC +MIS VD+DGDG VNF+EFKKMM+
Sbjct: 130 DCKKMISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of CSPI03G21520.1 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 160.2 bits (404), Expect = 1.3e-39
Identity = 78/146 (53.42%), Postives = 105/146 (71.92%), Query Frame = 0


Query: 11  TPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDG 70
           TP +  + + +E+ +VF++FD NGDGKISV EL      +  + +  E++R++ E+D D 
Sbjct: 12  TPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDR 71

Query: 71  DGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCC 130
           DG+I+LDEF+    SS+    +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC 
Sbjct: 72  DGYINLDEFSTLCRSSSSA-AEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCT 131

Query: 131 RMISSVDVDGDGHVNFEEFKKMMTRS 157
           RMI  VD DGDG+VNFEEF+KMMT S
Sbjct: 132 RMIGPVDADGDGNVNFEEFQKMMTSS 156

[Close]

BLAST of CSPI03G21520.1 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 160.2 bits (404), Expect = 1.3e-39
Identity = 78/146 (53.42%), Postives = 105/146 (71.92%), Query Frame = 0


Query: 11  TPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKDG 70
           TP +  + + +E+ +VF++FD NGDGKISV EL      +  + +  E++R++ E+D D 
Sbjct: 12  TPATVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDR 71

Query: 71  DGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDCC 130
           DG+I+LDEF+    SS+    +++DAFDLYD DKNGLISA ELH VL RLG  CS++DC 
Sbjct: 72  DGYINLDEFSTLCRSSSSA-AEIRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCT 131

Query: 131 RMISSVDVDGDGHVNFEEFKKMMTRS 157
           RMI  VD DGDG+VNFEEF+KMMT S
Sbjct: 132 RMIGPVDADGDGNVNFEEFQKMMTSS 156

[Close]

BLAST of CSPI03G21520.1 vs. TAIR 10
Match: AT1G73630.1 (EF hand calcium-binding protein family )

HSP 1 Score: 146.0 bits (367), Expect = 2.6e-35
Identity = 74/145 (51.03%), Postives = 103/145 (71.03%), Query Frame = 0


Query: 10  STPGSSALGSMDEIIRVFNKFDKNGDGKISVTELAAALGELSGNISTDEIHRIMSEIDKD 69
           ST  S+   +  E+ +VF+KFD NGDGKISV+EL      +  + + +E++R++ EID D
Sbjct: 8   STNKSTTPSTDMELKKVFDKFDANGDGKISVSELGNVFKSMGTSYTEEELNRVLDEIDID 67

Query: 70  GDGFIDLDEFTDFTSSSTGGNKDLQDAFDLYDIDKNGLISAKELHSVLKRLGEKCSLKDC 129
            DGFI+ +EF     SS+    ++++AFDLYD +KNGLIS+ E+H VL RLG  CS++DC
Sbjct: 68  CDGFINQEEFATICRSSSSA-VEIREAFDLYDQNKNGLISSSEIHKVLNRLGMTCSVEDC 127

Query: 130 CRMISSVDVDGDGHVNFEEFKKMMT 155
            RMI  VD DGDG+VNFEEF+KMM+
Sbjct: 128 VRMIGHVDTDGDGNVNFEEFQKMMS 151

The following BLAST results are available for this feature:

BLAST of CSPI03G21520.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C8Y1	9.0e-41	59.03	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]
P25070	1.5e-40	58.50	Calcium-binding protein CML24 OS=Arabidopsis thaliana OX=3702 GN=CML24 PE=2 SV=2	[more]
Q9LE22	1.9e-38	53.42	Probable calcium-binding protein CML27 OS=Arabidopsis thaliana OX=3702 GN=CML27 ...	[more]
Q9M7R0	3.0e-36	57.14	Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
Q9C9U8	3.6e-34	51.03	Probable calcium-binding protein CML26 OS=Arabidopsis thaliana OX=3702 GN=CML26 ...	[more]

BLAST of CSPI03G21520.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCM1	9.2e-81	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G319280 PE=4 SV=1	[more]
A0A5A7UHX3	1.4e-76	95.54	Putative calcium-binding protein CML27 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C5W9	1.4e-76	95.54	probable calcium-binding protein CML27 OS=Cucumis melo OX=3656 GN=LOC103496821 P...	[more]
A0A6J1G9H5	8.6e-63	80.63	probable calcium-binding protein CML23 OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1CNU4	1.1e-62	80.77	probable calcium-binding protein CML23 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of CSPI03G21520.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145834.1	1.9e-80	100.00	probable calcium-binding protein CML23 [Cucumis sativus]	[more]
XP_008457051.1	2.8e-76	95.54	PREDICTED: probable calcium-binding protein CML27 [Cucumis melo] >KAA0053141.1 p...	[more]
KAE8650604.1	5.9e-74	100.00	hypothetical protein Csa_010664 [Cucumis sativus]	[more]
XP_038879983.1	1.4e-67	87.90	probable calcium-binding protein CML23 [Benincasa hispida]	[more]
XP_022948508.1	1.8e-62	80.63	probable calcium-binding protein CML23 [Cucurbita moschata] >XP_023524703.1 prob...	[more]

BLAST of CSPI03G21520.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G66400.1	6.4e-42	59.03	calmodulin like 23	[more]
AT5G37770.1	1.1e-41	58.50	EF hand calcium-binding protein family	[more]
AT1G18210.1	1.3e-39	53.42	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	1.3e-39	53.42	Calcium-binding EF-hand family protein	[more]
AT1G73630.1	2.6e-35	51.03	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 92..120 e-value: 3.2E-5 score: 33.4 coord: 58..86 e-value: 2.1E-5 score: 34.0 coord: 22..50 e-value: 2.4E-5 score: 33.8 coord: 128..156 e-value: 2.1E-4 score: 30.6
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 91..153 e-value: 1.0E-11 score: 45.1 coord: 22..83 e-value: 1.1E-13 score: 51.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 124..156 score: 13.174425
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 88..123 score: 14.318138
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 18..53 score: 14.569197
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 54..82 score: 9.966455
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 22..82 e-value: 1.96872E-17 score: 69.8841
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 92..154 e-value: 5.88156E-19 score: 73.7361
None	No IPR available	GENE3D	1.10.238.10		coord: 14..88 e-value: 7.1E-21 score: 76.7
None	No IPR available	GENE3D	1.10.238.10		coord: 89..156 e-value: 9.5E-23 score: 82.3
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 15..155
None	No IPR available	PANTHER	PTHR10891:SF794	CALCIUM-BINDING PROTEIN CML23-RELATED	coord: 15..155
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 31..43
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 101..113
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 137..149
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 20..155

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G21520	CSPI03G21520	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G21520.1.utr3p1	CSPI03G21520.1.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G21520.1.cds1	CSPI03G21520.1.cds1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G21520.1.utr5p1	CSPI03G21520.1.utr5p1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G21520.1	CSPI03G21520.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding