CSPI03G17530.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI03G17530.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RING-type E3 ubiquitin transferase
Location	Chr3: 13146393 .. 13147972 (-)
Sequence length	1050
RNA-Seq Expression	CSPI03G17530.1
Synteny	CSPI03G17530.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTTAAAAATTCAAAGGACGAATCAACCCCTTCCTTTCGTCACAAAAGAAGGAATTTGATGAAGAACAAAAAACAAGTCCTCTTTCACAGTCGAAAAGCGAACTGATTTTACGATTCTTTATAGTTGAAGAAAAAGGAAAAGGAAAATGGAGGATTCTCGATCATCAGACGGCGGAAGTGAGAGGCGAAGTTCCGTCATTAGTAGGAGAGGGACGAACGGGACGTTGAACGTCATATTTACTGATCCGCAGATTCTCGATTGCTATATTTGTTGCGAACCCTTGTCAATTCCTGTTTTCCAGGTCTCATTAAAACTCTCTTCTCCTTTAAAATTGTGTTTATTGTTCATCTGGATACTACTGATGCTTTGTTTTTTCTCGCATAGCTTTTTTGGTTTTTGTTACTTTGGATACTGTAGTTTCGCTTGTGTGTGGTTGTTGCTGTTGACGATTTTAAAATCCCCTTTTCTTTGAGCTTTTCTCATTTTCTCTTTTCGAAGTTTATTGTTTAGACTATGCATTTCCATTGGAAGTAATTATAGATTGTTATGGAAATTCTGGAGTTATGATGTAAATAGTCTTAGACAATGGGAGAAAATTGCTTTACCTTGTTTATATTATCTTGAGTAAATGTTCGAATCAAACGACAGTTTATTTGATTTAACTTCAACTTTTCAATTGTATGGATTATTGGATTTTCTTCACTGTTTAGAAACATGAACTTGAAGGTATAGATATAGAAGAACATCAAGTTTATATTTGGGTTTATAGTCTCTTATATTCATGATTTTATTTGATCTCATAACATATACTTTGGTTGTTGGATGTATTAGTGTGAGAATGGGCATATTGCTTGCTCTAGCTGCTGCACCAAAGCTCAGAATAAATGCCCTAGCTGCACCTTAGCAATTGGCTACATACGTTGCAGGGCAATTGAGAAGGTTTTGGAATCAATCAAGTTACCATGTCAAAACGCAATATACGGATGCAAAACCGTGATGGGTTTGAACCTGATAAACGACCATGAGTCCTTATGCAGATATGAACCCTGCTCATGCCCTTTGGACAATTGCACCTTTGTTGGTTCAACAGAGCAGTTGGGCTTACACTTCACCAAAAAACACAAAGACTCAGCCAAAATCTTCTCATACAATACGAGGTTCACTATATGCTTGAACAATGGTGATACTCATCGTATCCTTAAAGCAGAGAATGATGGAGTTTTGTTCTTCCTATCTTACACATTTGAAATTTTTGGGAATGCTGTAACTATGAACCGGATTGGACCTTTGTCATCTGAGAAAAAGTTCTGTTATGAAATTAAAGCCAAAACACTGGGGAGTGTTCTTTCTTTACAATCAATAGCAAAGGAGATCCAAGGTTTGATAAAAGTTCCTCCTTCCAAGGGATCACTTTTGATTCCTAACGAGTACTTTGGATCTTCCACCCAGACTATGTTGGAGATTAGCATTTGGCCTGCACACTAAAGACAAAAACTGTGAATCAATAGCACGTTTGCTGATCTTCTTGGTTCATTGAACTTTGAATCTTTCTGGCTGCTTTTTTTTATCTTTTCAAAGG

mRNA sequence

ATTTAAAAATTCAAAGGACGAATCAACCCCTTCCTTTCGTCACAAAAGAAGGAATTTGATGAAGAACAAAAAACAAGTCCTCTTTCACAGTCGAAAAGCGAACTGATTTTACGATTCTTTATAGTTGAAGAAAAAGGAAAAGGAAAATGGAGGATTCTCGATCATCAGACGGCGGAAGTGAGAGGCGAAGTTCCGTCATTAGTAGGAGAGGGACGAACGGGACGTTGAACGTCATATTTACTGATCCGCAGATTCTCGATTGCTATATTTGTTGCGAACCCTTGTCAATTCCTGTTTTCCAGTGTGAGAATGGGCATATTGCTTGCTCTAGCTGCTGCACCAAAGCTCAGAATAAATGCCCTAGCTGCACCTTAGCAATTGGCTACATACGTTGCAGGGCAATTGAGAAGGTTTTGGAATCAATCAAGTTACCATGTCAAAACGCAATATACGGATGCAAAACCGTGATGGGTTTGAACCTGATAAACGACCATGAGTCCTTATGCAGATATGAACCCTGCTCATGCCCTTTGGACAATTGCACCTTTGTTGGTTCAACAGAGCAGTTGGGCTTACACTTCACCAAAAAACACAAAGACTCAGCCAAAATCTTCTCATACAATACGAGGTTCACTATATGCTTGAACAATGGTGATACTCATCGTATCCTTAAAGCAGAGAATGATGGAGTTTTGTTCTTCCTATCTTACACATTTGAAATTTTTGGGAATGCTGTAACTATGAACCGGATTGGACCTTTGTCATCTGAGAAAAAGTTCTGTTATGAAATTAAAGCCAAAACACTGGGGAGTGTTCTTTCTTTACAATCAATAGCAAAGGAGATCCAAGGTTTGATAAAAGTTCCTCCTTCCAAGGGATCACTTTTGATTCCTAACGAGTACTTTGGATCTTCCACCCAGACTATGTTGGAGATTAGCATTTGGCCTGCACACTAAAGACAAAAACTGTGAATCAATAGCACGTTTGCTGATCTTCTTGGTTCATTGAACTTTGAATCTTTCTGGCTGCTTTTTTTTATCTTTTCAAAGG

Coding sequence (CDS)

ATGGAGGATTCTCGATCATCAGACGGCGGAAGTGAGAGGCGAAGTTCCGTCATTAGTAGGAGAGGGACGAACGGGACGTTGAACGTCATATTTACTGATCCGCAGATTCTCGATTGCTATATTTGTTGCGAACCCTTGTCAATTCCTGTTTTCCAGTGTGAGAATGGGCATATTGCTTGCTCTAGCTGCTGCACCAAAGCTCAGAATAAATGCCCTAGCTGCACCTTAGCAATTGGCTACATACGTTGCAGGGCAATTGAGAAGGTTTTGGAATCAATCAAGTTACCATGTCAAAACGCAATATACGGATGCAAAACCGTGATGGGTTTGAACCTGATAAACGACCATGAGTCCTTATGCAGATATGAACCCTGCTCATGCCCTTTGGACAATTGCACCTTTGTTGGTTCAACAGAGCAGTTGGGCTTACACTTCACCAAAAAACACAAAGACTCAGCCAAAATCTTCTCATACAATACGAGGTTCACTATATGCTTGAACAATGGTGATACTCATCGTATCCTTAAAGCAGAGAATGATGGAGTTTTGTTCTTCCTATCTTACACATTTGAAATTTTTGGGAATGCTGTAACTATGAACCGGATTGGACCTTTGTCATCTGAGAAAAAGTTCTGTTATGAAATTAAAGCCAAAACACTGGGGAGTGTTCTTTCTTTACAATCAATAGCAAAGGAGATCCAAGGTTTGATAAAAGTTCCTCCTTCCAAGGGATCACTTTTGATTCCTAACGAGTACTTTGGATCTTCCACCCAGACTATGTTGGAGATTAGCATTTGGCCTGCACACTAA

Protein sequence

MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIACSSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLCRYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAENDGVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVPPSKGSLLIPNEYFGSSTQTMLEISIWPAH*

Homology

[Close]

BLAST of CSPI03G17530.1 vs. ExPASy Swiss-Prot
Match: Q84K34 (E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g37930 PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 1.9e-51
Identity = 107/281 (38.08%), Postives = 152/281 (54.09%), Query Frame = 0


Query: 2   EDSRSSDGGSERRSSVISRR------------GTNGTLNVIFTDPQILDCYICCEPLSIP 61
           ED  S+   S+R   +  RR              +  L+V   DP +LDC ICCEPL IP
Sbjct: 64  EDRGSTSDDSDREVVIEERRFGKFVNSQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKIP 123

Query: 62  VFQCENGHIACSSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMG 121
           +FQC+NGH+AC+ CCTK +N+CPSCTL IGY+RCRA+EKV+E+ ++ C NA YGCK    
Sbjct: 124 IFQCDNGHLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNAKYGCKESTS 183

Query: 122 LNLINDHESLCRYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNG 181
                 HE +C + PCSCP+ +C + G  + L  H   +HKD    F +NTR TI L+  
Sbjct: 184 YGNRFSHEQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNTRLTISLDLN 243

Query: 182 DTHRILKAENDGVLFFLSYTFEIFGNAVTMNRIGPLS-SEKKFCYEIKAKTLGSVLSLQS 241
               IL+ ENDG +  +     +    V+++ I PL+    +    +   T+ S+L    
Sbjct: 244 KKTTILQEENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQGF 303

Query: 242 IAKEIQGLIKVPPSKGSLLIPNEYFGSSTQTMLEISIWPAH 270
           + K IQ +    P  G +LIP+  F  +    L + IW  H
Sbjct: 304 MVKNIQKVTNEHPEDGFMLIPSYLFSGNDN--LNLQIWIGH 342

[Close]

BLAST of CSPI03G17530.1 vs. ExPASy Swiss-Prot
Match: Q9FKD9 (Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702 GN=At5g37870 PE=3 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.4e-38
Identity = 93/257 (36.19%), Postives = 141/257 (54.86%), Query Frame = 0


Query: 6   SSDGGSERR-----SSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 65
           S   GS++R     SS   R+  + T + + TD  ILDC IC + L IPVFQC NGH+AC
Sbjct: 7   SERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLAC 66

Query: 66  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 125
           SSCC K +NKCP+C L +G+IRCRA+E+VLES+ +PC+ A  GC   +     + HE +C
Sbjct: 67  SSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKIC 126

Query: 126 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYN--TRFTICLNNGDTHRILKAE 185
            + PCSCP+  C + GS + L  H+   H   +  +S+N  +     +   D   I +  
Sbjct: 127 NFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVY 186

Query: 186 NDGVLFFLSYTFEIFGNAVTMNRIGPLSSE-KKFCYEIKAKTL-GSVLSLQSIAKEIQGL 245
              +LF +    E  G  V+++ I P + E  +F Y +   T  G  ++ QS   +++ +
Sbjct: 187 EKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQS--PKVKKV 246

Query: 246 IKVP---PSKGSLLIPN 251
           +KV    P    +LIP+
Sbjct: 247 LKVSSQRPKDSFMLIPH 261

[Close]

BLAST of CSPI03G17530.1 vs. ExPASy Swiss-Prot
Match: Q9C9M0 (E3 ubiquitin-protein ligase SINA-like 4 OS=Arabidopsis thaliana OX=3702 GN=At1g66650 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 5.1e-36
Identity = 91/256 (35.55%), Postives = 132/256 (51.56%), Query Frame = 0


Query: 20  RRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIACSSCCTKAQNKCPSCTLAIG 79
           +R  +   +V   +  +L+C  C +PL  P+FQC NGH+AC  CC K + +C  C L IG
Sbjct: 67  KRRVSSPKSVTLPNSNVLECPNCFDPLKKPIFQCNNGHLACFLCCIKLKKRCSFCKLPIG 126

Query: 80  YIRCRAIEKVLESIKLPCQNAIYGCK--TVMGLNLINDHESLCRYEPCSCPLDNCTFVGS 139
            +RCRA+EKV+++  + C NAIYGCK  T  G N +  HE +C + PCSCP+ +C ++G 
Sbjct: 127 DVRCRAMEKVIKAGLVSCSNAIYGCKQSTTYG-NQLQSHEKVCVFAPCSCPIKDCNYIGF 186

Query: 140 TEQLGLHFTKKHKDS-----AKIFSYNTRFTICLNNGDTHRILKAENDGVLFFLSYTFEI 199
            + L  HF   HK S     + +F     F + L++ D   I   E  G LF +      
Sbjct: 187 YKDLINHFRATHKVSPGDINSFVFDRPVIFGLDLDSSDKMVIFVEEKQGNLFVVQGFIGS 246

Query: 200 FGNAVTMNRIGPLSSE-KKFCYEIKAKTLGSVLSLQSIAKEIQGL--IKVPPSKGSLLIP 259
            G   T++ I P+  E +KF   +      S L L    K IQ L   +  P +  LLIP
Sbjct: 247 HGVYATVSHIAPMVPEVRKFSCSLARLRPYSTLRLGLEVKNIQKLRSQEEQPQEDFLLIP 306

Query: 260 NEYFGSSTQTMLEISI 266
           + Y  +     +EISI
Sbjct: 307 S-YMVNGDHMKMEISI 320

[Close]

BLAST of CSPI03G17530.1 vs. ExPASy Swiss-Prot
Match: Q9FM14 (E3 ubiquitin-protein ligase SINA-like 11 OS=Arabidopsis thaliana OX=3702 GN=At5g62800 PE=2 SV=2)

HSP 1 Score: 151.8 bits (382), Expect = 1.1e-35
Identity = 96/264 (36.36%), Postives = 139/264 (52.65%), Query Frame = 0


Query: 1   MEDSRS--SDGGSERRSS--VISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENG 60
           MEDS S   +  S+R+SS     +R  N T +    D  +LDC +C EPL+IP FQC++G
Sbjct: 1   MEDSNSHPQNQTSKRKSSHPQKKQRMENETRSAKLLDLDVLDCPVCFEPLTIPTFQCDDG 60

Query: 61  HIACSSCCTKAQNKC--PSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLIN 120
           HI C+ C  K  NKC  P C L IG  RC A+E+VLES  +PCQN  +GC   +    ++
Sbjct: 61  HIVCNFCFAKVSNKCPGPGCDLPIGNKRCFAMERVLESAFVPCQNTEFGCTKSVSYEKVS 120

Query: 121 DHESLCRYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFS----YNTRFTICLNNGD 180
            HE  C Y  CSCP   C + GS   +  HF ++H  ++ I S    Y+T   + +N  +
Sbjct: 121 SHEKECNYSQCSCPNLECNYTGSYNIIYGHFMRRHLYNSTIVSSKWGYST-VDVLINIKE 180

Query: 181 THRILKAENDGVLFFLSYTFEIFGNAVTMNRIGPLSSE-KKFCYEIKAKTLGSVLSLQSI 240
              +L      +LF +    E  G  VT+ RI P +SE KKF Y +     G  ++ +S 
Sbjct: 181 KVSVLWESRQKLLFVVQCFKERHGVYVTVRRIAPPASEFKKFSYRLSYSIDGHNVTYES- 240

Query: 241 AKEIQGLIKVP---PSKGSLLIPN 251
             E++ L++V    P    + +PN
Sbjct: 241 -PEVKRLLEVNSQIPDDSFMFVPN 261

[Close]

BLAST of CSPI03G17530.1 vs. ExPASy Swiss-Prot
Match: Q9FKD7 (E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g37890 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 1.1e-35
Identity = 83/229 (36.24%), Postives = 127/229 (55.46%), Query Frame = 0


Query: 28  NVIFTDPQILDCYICCEPLSIPVFQCENGHIACSSCCTKAQNKCPSCTLAIGYIRCRAIE 87
           + +  D +ILDC IC E  +IP+FQC+NGH+ACSSCC K  NKCP+CT  +G+ RCRA+E
Sbjct: 40  STMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNNKCPACTSPVGHNRCRAME 99

Query: 88  KVLESIKLPCQNAIYGCKTVMGLNLINDHESLCRYEPCSCPLDNCTFVGSTEQLGLHFTK 147
            VLESI +PC NA  GCK  +       HE  C +  C+CP  +C +  S + L  H+  
Sbjct: 100 SVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPALDCNYTSSYKDLYTHYRI 159

Query: 148 KHKDSAKIFSYNTRFTICLNNGDTHRIL-KAEN-DGVLFFLSYTFEIFGNAVTMNRIGPL 207
            H +  +I ++     + +    + +IL + E+    LF +    E +G  VT++ I P 
Sbjct: 160 THMEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQCFREPYGVYVTVSCIAPS 219

Query: 208 SSE-KKFCYEIKAKTLGSVLSLQSIAKEIQGLIKV---PPSKGSLLIPN 251
           S E  ++ Y +     G  +  QS   E++ ++K+    P +  +LIPN
Sbjct: 220 SPELSQYSYALSYTVDGHTVIYQS--PEVKRVLKLSFQTPQENFMLIPN 266

[Close]

BLAST of CSPI03G17530.1 vs. ExPASy TrEMBL
Match: A0A0A0L9Z6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G185660 PE=3 SV=1)

HSP 1 Score: 560.5 bits (1443), Expect = 3.9e-156
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 0


Query: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60
           MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC
Sbjct: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60

Query: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120
           SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC
Sbjct: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120

Query: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180
           RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND
Sbjct: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180

Query: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240
           GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP
Sbjct: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240

Query: 241 PSKGSLLIPNEYFGSSTQTMLEISIWPAH 270
           PSKGSLLIPNEYFGSSTQTMLEISIWPAH
Sbjct: 241 PSKGSLLIPNEYFGSSTQTMLEISIWPAH 269

[Close]

BLAST of CSPI03G17530.1 vs. ExPASy TrEMBL
Match: A0A6J1CNI1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111013226 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.3e-116
Identity = 201/269 (74.72%), Postives = 232/269 (86.25%), Query Frame = 0


Query: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60
           M DSR+SDG   +RS V S RG    +NVIF+DPQ+LDCYICCEPLS PVFQCENGHIAC
Sbjct: 1   MADSRASDGERGKRSPV-SGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIAC 60

Query: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120
           SSCCTK QNKCPSC+L+IGYIRCRAIEKVLESIK+PC N+IYGCK  M LN  NDH SLC
Sbjct: 61  SSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLC 120

Query: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180
            YEPCSCP+ +C FVGS+E+L  HF+KKHK+SAK FSYN+RFT+CLN+GD++RIL+AE D
Sbjct: 121 TYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKD 180

Query: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240
           G LFFLS+TFEIFGNAVT++RIGP SSEKKFCYEIKAKT GS+L LQSIAKEIQG ++VP
Sbjct: 181 GALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVP 240

Query: 241 PSKGSLLIPNEYFGSSTQTMLEISIWPAH 270
           PS  SLLIPNE+FGSS QT+LE  IWP++
Sbjct: 241 PSNRSLLIPNEFFGSSAQTILEFRIWPSY 268

[Close]

BLAST of CSPI03G17530.1 vs. ExPASy TrEMBL
Match: A0A6J1K8G5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492642 PE=3 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.3e-111
Identity = 194/269 (72.12%), Postives = 225/269 (83.64%), Query Frame = 0


Query: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60
           M DSRSSDG  ERR SV   RG N  + VIFTDPQILDCYICCEPLS+PV+QCENGHI+C
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120
           SSCC+K  NKCPSC+LAIGYIRCRAIEKVLESIK+PCQN+ YGCK  M LN IN+HE+ C
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSKYGCKITMNLNQINEHETSC 120

Query: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180
            YEPCSCP+ +C++V S E LG HF K+H+DSAK F YN+RFTICLN+GD +RI +AEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240
             LF LSY+FEIFGNAVT++RIG   SEKKFCY+IKAK+ GSVLSLQS+AKEIQ   + P
Sbjct: 181 DALFVLSYSFEIFGNAVTLSRIGSPLSEKKFCYDIKAKSQGSVLSLQSVAKEIQRWTEDP 240

Query: 241 PSKGSLLIPNEYFGSST-QTMLEISIWPA 269
           P KGSLLIPNE+FGSS+ QT+LEI IWP+
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of CSPI03G17530.1 vs. ExPASy TrEMBL
Match: A0A6J1GBP1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452508 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 6.6e-111
Identity = 193/269 (71.75%), Postives = 226/269 (84.01%), Query Frame = 0


Query: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60
           M  SRSSDG  ERRSS  S R  N T+ VI TDPQ+LDCYICCEPLS+PV+QCENGHI+C
Sbjct: 271 MAGSRSSDGEKERRSSANSSRRKNETMKVILTDPQVLDCYICCEPLSVPVYQCENGHISC 330

Query: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120
           SSCC+K QNKCPSC+LAIGY+RCRAIEKVLESIK+PCQN+ YGCKT   LN IN+HE+LC
Sbjct: 331 SSCCSKVQNKCPSCSLAIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 390

Query: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180
            Y+PCSCPL +C++V S E L  HF K+H+DSAK  SYN+RFTICLN+ D + I +AEND
Sbjct: 391 PYQPCSCPLSDCSYVDSAEYLSSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEND 450

Query: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240
           GVLF LSY+FE FG+AVT+NRIGP SSEKKFCY+IKAK+ GSVLSL S+AKEIQ   + P
Sbjct: 451 GVLFVLSYSFETFGDAVTLNRIGPRSSEKKFCYDIKAKSQGSVLSLLSVAKEIQRWTEDP 510

Query: 241 PSKGSLLIPNEYFGSST-QTMLEISIWPA 269
           PSKGSLLIPNE+FGSS+ QT+LEI IWP+
Sbjct: 511 PSKGSLLIPNEFFGSSSAQTILEIRIWPS 539

[Close]

BLAST of CSPI03G17530.1 vs. ExPASy TrEMBL
Match: A0A6J1KAW2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 PE=3 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 4.7e-109
Identity = 190/269 (70.63%), Postives = 224/269 (83.27%), Query Frame = 0


Query: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60
           M  SRSSDG  ERRSSV S R  N T+ VI TDPQ+LDCYICC PLS+PV+QCENGHI+C
Sbjct: 1   MAGSRSSDGEKERRSSVNSSRWKNETMKVILTDPQVLDCYICCVPLSVPVYQCENGHISC 60

Query: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120
           SSCC+K  NKCPSC+L IGY+RCRAIEKVLESIK+PCQN+ YGCKT   LN IN+HE+LC
Sbjct: 61  SSCCSKVHNKCPSCSLPIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 120

Query: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180
            Y+PCSCPL +C++V S E LG HF K+H+DSAK  SYN+RFTICLN+ D + I +AEND
Sbjct: 121 PYQPCSCPLSDCSYVDSAEHLGSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEND 180

Query: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240
           GVLF LSY+FE FG+AVT+ RIGP SSEKKFCY+IKAK+ G+VLSL S+AKEIQ   + P
Sbjct: 181 GVLFVLSYSFETFGDAVTLKRIGPRSSEKKFCYDIKAKSHGNVLSLLSVAKEIQRWTEDP 240

Query: 241 PSKGSLLIPNEYFGSST-QTMLEISIWPA 269
           PSKGSLLIPNE+FGSS+ QT+LEI IWP+
Sbjct: 241 PSKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of CSPI03G17530.1 vs. NCBI nr
Match: XP_004140738.2 (E3 ubiquitin-protein ligase SINA-like 10 [Cucumis sativus] >KGN57437.1 hypothetical protein Csa_011184 [Cucumis sativus])

HSP 1 Score: 560.5 bits (1443), Expect = 8.1e-156
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 0


Query: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60
           MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC
Sbjct: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60

Query: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120
           SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC
Sbjct: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120

Query: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180
           RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND
Sbjct: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180

Query: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240
           GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP
Sbjct: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240

Query: 241 PSKGSLLIPNEYFGSSTQTMLEISIWPAH 270
           PSKGSLLIPNEYFGSSTQTMLEISIWPAH
Sbjct: 241 PSKGSLLIPNEYFGSSTQTMLEISIWPAH 269

[Close]

BLAST of CSPI03G17530.1 vs. NCBI nr
Match: XP_038893917.1 (E3 ubiquitin-protein ligase SINA-like 10 [Benincasa hispida] >XP_038893986.1 E3 ubiquitin-protein ligase SINA-like 10 [Benincasa hispida])

HSP 1 Score: 438.0 bits (1125), Expect = 6.1e-119
Identity = 211/269 (78.44%), Postives = 233/269 (86.62%), Query Frame = 0


Query: 1   MEDSRSSDGGSERRSSVI-SRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIA 60
           M DSR SD  S+RR S+I S R  N  +NVIFTDPQILDCYICCE LSIPVFQCENGHI+
Sbjct: 1   MADSRGSDSESDRRRSLISSNRRKNEAMNVIFTDPQILDCYICCERLSIPVFQCENGHIS 60

Query: 61  CSSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESL 120
           CSSCCTK QNKCPSC LAIG IRCRAIEKVLESIK+PC+NAIYGCK  M LN I DHE+ 
Sbjct: 61  CSSCCTKIQNKCPSCNLAIGNIRCRAIEKVLESIKIPCRNAIYGCKMTMSLNEI-DHETS 120

Query: 121 CRYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEN 180
           C +EPC CPLD+CTFVGS E+L LHF+KKHK+++K FSYN RFT+CLNNGD+HRIL+AEN
Sbjct: 121 CTHEPCFCPLDDCTFVGSAEKLSLHFSKKHKNTSKSFSYNARFTMCLNNGDSHRILQAEN 180

Query: 181 DGVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKV 240
           D +LF LSY+FEIFGNAVT+NRIGPLSSE+KFCYEIKAKT GSVL  QSIAKEIQG  KV
Sbjct: 181 DSILFVLSYSFEIFGNAVTVNRIGPLSSERKFCYEIKAKTQGSVLCFQSIAKEIQGWSKV 240

Query: 241 PPSKGSLLIPNEYFGSSTQTMLEISIWPA 269
           PPSKG LLIPNEYFGSSTQT+LEI IWPA
Sbjct: 241 PPSKGWLLIPNEYFGSSTQTILEIRIWPA 268

[Close]

BLAST of CSPI03G17530.1 vs. NCBI nr
Match: XP_022143325.1 (E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia])

HSP 1 Score: 428.3 bits (1100), Expect = 4.8e-116
Identity = 201/269 (74.72%), Postives = 232/269 (86.25%), Query Frame = 0


Query: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60
           M DSR+SDG   +RS V S RG    +NVIF+DPQ+LDCYICCEPLS PVFQCENGHIAC
Sbjct: 1   MADSRASDGERGKRSPV-SGRGKTEAMNVIFSDPQLLDCYICCEPLSAPVFQCENGHIAC 60

Query: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120
           SSCCTK QNKCPSC+L+IGYIRCRAIEKVLESIK+PC N+IYGCK  M LN  NDH SLC
Sbjct: 61  SSCCTKVQNKCPSCSLSIGYIRCRAIEKVLESIKIPCHNSIYGCKMTMTLNERNDHGSLC 120

Query: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180
            YEPCSCP+ +C FVGS+E+L  HF+KKHK+SAK FSYN+RFT+CLN+GD++RIL+AE D
Sbjct: 121 TYEPCSCPVSDCLFVGSSEKLCSHFSKKHKNSAKSFSYNSRFTLCLNSGDSYRILQAEKD 180

Query: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240
           G LFFLS+TFEIFGNAVT++RIGP SSEKKFCYEIKAKT GS+L LQSIAKEIQG ++VP
Sbjct: 181 GALFFLSFTFEIFGNAVTVSRIGPSSSEKKFCYEIKAKTQGSILCLQSIAKEIQGRVEVP 240

Query: 241 PSKGSLLIPNEYFGSSTQTMLEISIWPAH 270
           PS  SLLIPNE+FGSS QT+LE  IWP++
Sbjct: 241 PSNRSLLIPNEFFGSSAQTILEFRIWPSY 268

[Close]

BLAST of CSPI03G17530.1 vs. NCBI nr
Match: XP_022997791.1 (E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima])

HSP 1 Score: 412.5 bits (1059), Expect = 2.7e-111
Identity = 194/269 (72.12%), Postives = 225/269 (83.64%), Query Frame = 0


Query: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60
           M DSRSSDG  ERR SV   RG N  + VIFTDPQILDCYICCEPLS+PV+QCENGHI+C
Sbjct: 1   MADSRSSDGEKERRRSVNCSRGKNEAMKVIFTDPQILDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120
           SSCC+K  NKCPSC+LAIGYIRCRAIEKVLESIK+PCQN+ YGCK  M LN IN+HE+ C
Sbjct: 61  SSCCSKVHNKCPSCSLAIGYIRCRAIEKVLESIKIPCQNSKYGCKITMNLNQINEHETSC 120

Query: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180
            YEPCSCP+ +C++V S E LG HF K+H+DSAK F YN+RFTICLN+GD +RI +AEND
Sbjct: 121 PYEPCSCPISDCSYVDSAEHLGSHFRKRHQDSAKSFLYNSRFTICLNSGDRYRIFQAEND 180

Query: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240
             LF LSY+FEIFGNAVT++RIG   SEKKFCY+IKAK+ GSVLSLQS+AKEIQ   + P
Sbjct: 181 DALFVLSYSFEIFGNAVTLSRIGSPLSEKKFCYDIKAKSQGSVLSLQSVAKEIQRWTEDP 240

Query: 241 PSKGSLLIPNEYFGSST-QTMLEISIWPA 269
           P KGSLLIPNE+FGSS+ QT+LEI IWP+
Sbjct: 241 PFKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of CSPI03G17530.1 vs. NCBI nr
Match: KAG6606997.1 (E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 410.2 bits (1053), Expect = 1.4e-110
Identity = 193/269 (71.75%), Postives = 226/269 (84.01%), Query Frame = 0


Query: 1   MEDSRSSDGGSERRSSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 60
           M  SRSSDG  ERRSS  S R  N T+ VI TDPQ+LDCYICCEPLS+PV+QCENGHI+C
Sbjct: 1   MAGSRSSDGEKERRSSANSSRRKNETMKVILTDPQVLDCYICCEPLSVPVYQCENGHISC 60

Query: 61  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 120
           SSCC+K QNKCPSC+LAIGY+RCRAIEKVLESIK+PCQN+ YGCKT   LN IN+HE+LC
Sbjct: 61  SSCCSKVQNKCPSCSLAIGYMRCRAIEKVLESIKMPCQNSRYGCKTTTNLNQINEHETLC 120

Query: 121 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNGDTHRILKAEND 180
            Y+PCSCPL +C++V S E L  HF K+H+DSAK  SYN+RFTICLN+ D + I +AEND
Sbjct: 121 PYQPCSCPLSDCSYVDSAEYLSSHFRKRHQDSAKSISYNSRFTICLNSDDKYCIFQAEND 180

Query: 181 GVLFFLSYTFEIFGNAVTMNRIGPLSSEKKFCYEIKAKTLGSVLSLQSIAKEIQGLIKVP 240
           GVLF LSY+FE FG+AVT+NRIGP SSEKKFCY+IKAK+ GSVLSL S+AKEIQ   + P
Sbjct: 181 GVLFVLSYSFETFGDAVTLNRIGPRSSEKKFCYDIKAKSQGSVLSLLSVAKEIQRWTEDP 240

Query: 241 PSKGSLLIPNEYFGSST-QTMLEISIWPA 269
           PSKGSLLIPNE+FGSS+ QT+LEI IWP+
Sbjct: 241 PSKGSLLIPNEFFGSSSAQTILEIRIWPS 269

[Close]

BLAST of CSPI03G17530.1 vs. TAIR 10
Match: AT5G37930.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 204.1 bits (518), Expect = 1.4e-52
Identity = 107/281 (38.08%), Postives = 152/281 (54.09%), Query Frame = 0


Query: 2   EDSRSSDGGSERRSSVISRR------------GTNGTLNVIFTDPQILDCYICCEPLSIP 61
           ED  S+   S+R   +  RR              +  L+V   DP +LDC ICCEPL IP
Sbjct: 64  EDRGSTSDDSDREVVIEERRFGKFVNSQSSSSSKDSPLSVTLLDPDVLDCPICCEPLKIP 123

Query: 62  VFQCENGHIACSSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMG 121
           +FQC+NGH+AC+ CCTK +N+CPSCTL IGY+RCRA+EKV+E+ ++ C NA YGCK    
Sbjct: 124 IFQCDNGHLACTLCCTKVRNRCPSCTLPIGYVRCRAMEKVIEASRVSCLNAKYGCKESTS 183

Query: 122 LNLINDHESLCRYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYNTRFTICLNNG 181
                 HE +C + PCSCP+ +C + G  + L  H   +HKD    F +NTR TI L+  
Sbjct: 184 YGNRFSHEQVCVFTPCSCPILDCHYTGYYKDLNNHVRAEHKDDLISFVWNTRLTISLDLN 243

Query: 182 DTHRILKAENDGVLFFLSYTFEIFGNAVTMNRIGPLS-SEKKFCYEIKAKTLGSVLSLQS 241
               IL+ ENDG +  +     +    V+++ I PL+    +    +   T+ S+L    
Sbjct: 244 KKTTILQEENDGHVIVVQVFRALHAVYVSVSCIAPLTPGVGRLSCRLAKITVDSLLKQGF 303

Query: 242 IAKEIQGLIKVPPSKGSLLIPNEYFGSSTQTMLEISIWPAH 270
           + K IQ +    P  G +LIP+  F  +    L + IW  H
Sbjct: 304 MVKNIQKVTNEHPEDGFMLIPSYLFSGNDN--LNLQIWIGH 342

[Close]

BLAST of CSPI03G17530.1 vs. TAIR 10
Match: AT5G37870.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 161.4 bits (407), Expect = 1.0e-39
Identity = 93/257 (36.19%), Postives = 141/257 (54.86%), Query Frame = 0


Query: 6   SSDGGSERR-----SSVISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIAC 65
           S   GS++R     SS   R+  + T + + TD  ILDC IC + L IPVFQC NGH+AC
Sbjct: 7   SERNGSQKRHCSSISSDDGRKRVDKTRSAMLTDLDILDCPICYQALKIPVFQCGNGHLAC 66

Query: 66  SSCCTKAQNKCPSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLINDHESLC 125
           SSCC K +NKCP+C L +G+IRCRA+E+VLES+ +PC+ A  GC   +     + HE +C
Sbjct: 67  SSCCPKLRNKCPACALPVGHIRCRAMERVLESVLVPCRYADLGCTKTIYYGRESTHEKIC 126

Query: 126 RYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFSYN--TRFTICLNNGDTHRILKAE 185
            + PCSCP+  C + GS + L  H+   H   +  +S+N  +     +   D   I +  
Sbjct: 127 NFSPCSCPVQGCNYTGSYKDLYEHYDLTHSTGSTAYSFNGVSYIAAMMFISDKILIERVY 186

Query: 186 NDGVLFFLSYTFEIFGNAVTMNRIGPLSSE-KKFCYEIKAKTL-GSVLSLQSIAKEIQGL 245
              +LF +    E  G  V+++ I P + E  +F Y +   T  G  ++ QS   +++ +
Sbjct: 187 EKKLLFVVQCFEEPCGVYVSVSCIAPSAPEVGEFSYGLLYTTWEGVTMTYQS--PKVKKV 246

Query: 246 IKVP---PSKGSLLIPN 251
           +KV    P    +LIP+
Sbjct: 247 LKVSSQRPKDSFMLIPH 261

[Close]

BLAST of CSPI03G17530.1 vs. TAIR 10
Match: AT1G66650.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 152.9 bits (385), Expect = 3.6e-37
Identity = 91/256 (35.55%), Postives = 132/256 (51.56%), Query Frame = 0


Query: 20  RRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENGHIACSSCCTKAQNKCPSCTLAIG 79
           +R  +   +V   +  +L+C  C +PL  P+FQC NGH+AC  CC K + +C  C L IG
Sbjct: 67  KRRVSSPKSVTLPNSNVLECPNCFDPLKKPIFQCNNGHLACFLCCIKLKKRCSFCKLPIG 126

Query: 80  YIRCRAIEKVLESIKLPCQNAIYGCK--TVMGLNLINDHESLCRYEPCSCPLDNCTFVGS 139
            +RCRA+EKV+++  + C NAIYGCK  T  G N +  HE +C + PCSCP+ +C ++G 
Sbjct: 127 DVRCRAMEKVIKAGLVSCSNAIYGCKQSTTYG-NQLQSHEKVCVFAPCSCPIKDCNYIGF 186

Query: 140 TEQLGLHFTKKHKDS-----AKIFSYNTRFTICLNNGDTHRILKAENDGVLFFLSYTFEI 199
            + L  HF   HK S     + +F     F + L++ D   I   E  G LF +      
Sbjct: 187 YKDLINHFRATHKVSPGDINSFVFDRPVIFGLDLDSSDKMVIFVEEKQGNLFVVQGFIGS 246

Query: 200 FGNAVTMNRIGPLSSE-KKFCYEIKAKTLGSVLSLQSIAKEIQGL--IKVPPSKGSLLIP 259
            G   T++ I P+  E +KF   +      S L L    K IQ L   +  P +  LLIP
Sbjct: 247 HGVYATVSHIAPMVPEVRKFSCSLARLRPYSTLRLGLEVKNIQKLRSQEEQPQEDFLLIP 306

Query: 260 NEYFGSSTQTMLEISI 266
           + Y  +     +EISI
Sbjct: 307 S-YMVNGDHMKMEISI 320

[Close]

BLAST of CSPI03G17530.1 vs. TAIR 10
Match: AT5G62800.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 151.8 bits (382), Expect = 8.1e-37
Identity = 96/264 (36.36%), Postives = 139/264 (52.65%), Query Frame = 0


Query: 1   MEDSRS--SDGGSERRSS--VISRRGTNGTLNVIFTDPQILDCYICCEPLSIPVFQCENG 60
           MEDS S   +  S+R+SS     +R  N T +    D  +LDC +C EPL+IP FQC++G
Sbjct: 1   MEDSNSHPQNQTSKRKSSHPQKKQRMENETRSAKLLDLDVLDCPVCFEPLTIPTFQCDDG 60

Query: 61  HIACSSCCTKAQNKC--PSCTLAIGYIRCRAIEKVLESIKLPCQNAIYGCKTVMGLNLIN 120
           HI C+ C  K  NKC  P C L IG  RC A+E+VLES  +PCQN  +GC   +    ++
Sbjct: 61  HIVCNFCFAKVSNKCPGPGCDLPIGNKRCFAMERVLESAFVPCQNTEFGCTKSVSYEKVS 120

Query: 121 DHESLCRYEPCSCPLDNCTFVGSTEQLGLHFTKKHKDSAKIFS----YNTRFTICLNNGD 180
            HE  C Y  CSCP   C + GS   +  HF ++H  ++ I S    Y+T   + +N  +
Sbjct: 121 SHEKECNYSQCSCPNLECNYTGSYNIIYGHFMRRHLYNSTIVSSKWGYST-VDVLINIKE 180

Query: 181 THRILKAENDGVLFFLSYTFEIFGNAVTMNRIGPLSSE-KKFCYEIKAKTLGSVLSLQSI 240
              +L      +LF +    E  G  VT+ RI P +SE KKF Y +     G  ++ +S 
Sbjct: 181 KVSVLWESRQKLLFVVQCFKERHGVYVTVRRIAPPASEFKKFSYRLSYSIDGHNVTYES- 240

Query: 241 AKEIQGLIKVP---PSKGSLLIPN 251
             E++ L++V    P    + +PN
Sbjct: 241 -PEVKRLLEVNSQIPDDSFMFVPN 261

[Close]

BLAST of CSPI03G17530.1 vs. TAIR 10
Match: AT5G37890.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 151.8 bits (382), Expect = 8.1e-37
Identity = 83/229 (36.24%), Postives = 127/229 (55.46%), Query Frame = 0


Query: 28  NVIFTDPQILDCYICCEPLSIPVFQCENGHIACSSCCTKAQNKCPSCTLAIGYIRCRAIE 87
           + +  D +ILDC IC E  +IP+FQC+NGH+ACSSCC K  NKCP+CT  +G+ RCRA+E
Sbjct: 40  STMLMDLEILDCPICYEAFTIPIFQCDNGHLACSSCCPKLNNKCPACTSPVGHNRCRAME 99

Query: 88  KVLESIKLPCQNAIYGCKTVMGLNLINDHESLCRYEPCSCPLDNCTFVGSTEQLGLHFTK 147
            VLESI +PC NA  GCK  +       HE  C +  C+CP  +C +  S + L  H+  
Sbjct: 100 SVLESILIPCPNAKLGCKKNVSYGKELTHEKECMFSHCACPALDCNYTSSYKDLYTHYRI 159

Query: 148 KHKDSAKIFSYNTRFTICLNNGDTHRIL-KAEN-DGVLFFLSYTFEIFGNAVTMNRIGPL 207
            H +  +I ++     + +    + +IL + E+    LF +    E +G  VT++ I P 
Sbjct: 160 THMEINQINTFICDIPLSVRMNISKKILIRTEHLTNHLFAVQCFREPYGVYVTVSCIAPS 219

Query: 208 SSE-KKFCYEIKAKTLGSVLSLQSIAKEIQGLIKV---PPSKGSLLIPN 251
           S E  ++ Y +     G  +  QS   E++ ++K+    P +  +LIPN
Sbjct: 220 SPELSQYSYALSYTVDGHTVIYQS--PEVKRVLKLSFQTPQENFMLIPN 266

The following BLAST results are available for this feature:

BLAST of CSPI03G17530.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84K34	1.9e-51	38.08	E3 ubiquitin-protein ligase SINA-like 10 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
Q9FKD9	1.4e-38	36.19	Putative E3 ubiquitin-protein ligase SINA-like 6 OS=Arabidopsis thaliana OX=3702...	[more]
Q9C9M0	5.1e-36	35.55	E3 ubiquitin-protein ligase SINA-like 4 OS=Arabidopsis thaliana OX=3702 GN=At1g6...	[more]
Q9FM14	1.1e-35	36.36	E3 ubiquitin-protein ligase SINA-like 11 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
Q9FKD7	1.1e-35	36.24	E3 ubiquitin-protein ligase SINA-like 7 OS=Arabidopsis thaliana OX=3702 GN=At5g3...	[more]

BLAST of CSPI03G17530.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9Z6	3.9e-156	100.00	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G185660 PE...	[more]
A0A6J1CNI1	2.3e-116	74.72	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101322...	[more]
A0A6J1K8G5	1.3e-111	72.12	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492642 P...	[more]
A0A6J1GBP1	6.6e-111	71.75	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111452508...	[more]
A0A6J1KAW2	4.7e-109	70.63	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492641 P...	[more]

BLAST of CSPI03G17530.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140738.2	8.1e-156	100.00	E3 ubiquitin-protein ligase SINA-like 10 [Cucumis sativus] >KGN57437.1 hypotheti...	[more]
XP_038893917.1	6.1e-119	78.44	E3 ubiquitin-protein ligase SINA-like 10 [Benincasa hispida] >XP_038893986.1 E3 ...	[more]
XP_022143325.1	4.8e-116	74.72	E3 ubiquitin-protein ligase SINA-like 10 [Momordica charantia]	[more]
XP_022997791.1	2.7e-111	72.12	E3 ubiquitin-protein ligase SINA-like 10 [Cucurbita maxima]	[more]
KAG6606997.1	1.4e-110	71.75	E3 ubiquitin-protein ligase SINA-like 10, partial [Cucurbita argyrosperma subsp....	[more]

BLAST of CSPI03G17530.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G37930.1	1.4e-52	38.08	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37870.1	1.0e-39	36.19	Protein with RING/U-box and TRAF-like domains	[more]
AT1G66650.1	3.6e-37	35.55	Protein with RING/U-box and TRAF-like domains	[more]
AT5G62800.1	8.1e-37	36.36	Protein with RING/U-box and TRAF-like domains	[more]
AT5G37890.1	8.1e-37	36.24	Protein with RING/U-box and TRAF-like domains	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 81..234 e-value: 1.0E-18 score: 68.1
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 87..145 e-value: 8.3E-16 score: 60.0
IPR044286	E3 ubiquitin-protein ligase SINA-like, plant	PANTHER	PTHR46632	E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4	coord: 9..267
None	No IPR available	PANTHER	PTHR46632:SF16	E3 UBIQUITIN-PROTEIN LIGASE SINA-LIKE 4	coord: 9..267
None	No IPR available	SUPERFAMILY	49599	TRAF domain-like	coord: 92..254
IPR013010	Zinc finger, SIAH-type	PROSITE	PS51081	ZF_SIAH	coord: 92..150 score: 13.26399

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G17530	CSPI03G17530	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G17530.1.utr3p1	CSPI03G17530.1.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G17530.1.cds2	CSPI03G17530.1.cds2	CDS
CSPI03G17530.1.cds1	CSPI03G17530.1.cds1	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G17530.1.utr5p1	CSPI03G17530.1.utr5p1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G17530.1	CSPI03G17530.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007275	multicellular organism development
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0016740	transferase activity