CSPI03G08990.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI03G08990.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein
Location	Chr3: 7409483 .. 7413636 (-)
Sequence length	1534
RNA-Seq Expression	CSPI03G08990.1
Synteny	CSPI03G08990.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAGTGTACTAAAGAGGAAACTGAAGGGTGGGAGATGGAATTTTCGTCGCTTCATAAACGTGCAGTGCCGGCGTTTGGATCTCCGATTTCAACTACGCCTGCCTTTCCCGCCGGAACTCCTTCTTCCACCTTACACACTCTTTGATCGTCAGAGAATGGCCGCCGAAGCTCAAGACGAAGCTCATAGATTCTTCGTTGCAGTTCATGTTGGCGCGGGATTTCATGCTCCTTCCAACGAGAAAGCCCTCAGATCAGCCATGAAACGCGCTTGTTTAGCTGCCGCTGTAGTTCTTCGAAAGGTTTGTTTCGCTCTCGTTTTTCCCAAAGACCGACGCATTTTGAATTTTCACTCCATAGGAATTGAAACTATAACTGCCGAATCCGTAGCTTTTCTCAATCCAATTGAATGTGCTAAGCGAATTAGATTCATTGTTCGACGTGATCATAGAAATGTGTTTCTTTTTAATGTGTTGTTGGAATGTATAGAGTTTTGGAGAAGTTCATGGGATTACAGTCATTTACTGTTTTATTTTCGAGTAGTTTCTATTTGAAGTTCGTTGTAGCCTTGTGAAATCCTTATGGTGTTGTTGTTTTGTTTTAGGGTTCAGGCGGCTGTTTAGATGCTGTTTCCGCTGCTATAAAAGTTCTAGAGGTAGCTTTCCCTTTCAAATATCTCCTTCATTTTATTTCTTTCTAGTATTTTAGTGTAATTTTACCACCGGAAATTTATGGTTTGCAACATAAAAATAGCTATTCAAAAGTGGTTGTTGTACAATAACATGAAAATCATTTTTGTCAATTTCAAAATCACTCAAAAAATGTTTTTAAACATTCAAGATCTAGTAGGACTGTGCCTTTGTTTTTCTTGAGCAAAACAAATTTGAATAGTATAGAAAATGATCTTGAAAAGTGTTGAGTGACTAAAAGTATGTTTACAGAGTGATTTTAAACATGACAAAGTGATCCATACAAGATTATCTAGTGAGAAAAAAAATGTTTGTACCAGTCTTACTAGGATTGTGTCTTTTGGTGGTACAGCTTTCATATATTTTGTAGGTGTTAATTCTCCAATTGTTTTCTCTAACACGGGTACCGGTTGTTCATCTTCTGCTGGATAAACATGCCTTTGAATTCCTGAATTTACAAAGAAAATTTTATTGTAGGATGATCCAGCGACAAATGCTGGACGAGGCTCAAATTTGACGGATGATGGTCAAGTAGAATGTGATGCTAGTATTATGGATGGTGATACAGGTGCTTTTGGAGCTGTTGGTGCAGTGCCAGGTTTATCATTCTCTAATGATGAAACTTTTTGTTATCTCTCATCAACATTGGGTTCTGGGTCATTTTGATGTTCGGATATTTATGTATAACTGCATCTTTGTTTTCAAAATTTTAATAGCAATGTACTTATTCTGTTAATTTAATTGATATCTATCATAATATGTTCTAGACCAGGTGTCCGAAATGCTATCCAAATTGCTACTTTACTAGCAAAGGAACAAATACTGGGATCATCTCTGCTGGGCCTTATACCTCCGATGTAATTATTATTGTGAGATTGCATTGCTTCATTTTCTTTTCTTCCAGTGGAGCATTTTTCCTACTACAGGATATTGTTTGTCATGTGATAGACCTTAGTTGATCGGTGCCACGTAATAACAAAGAAAATTAGTGTCAAAACACAATATAGTCTCAGCAGTCAGTAATCCAGAGGCCAAGTTGTATCCTTCCTAGAGCAGGGTCCAGGCAAAAAGGTCTTACTAACTCTATGTCCAAGATACGTATGTGGCCTATTATCATGTCACCCTATTACAAAATCAGGTCCACACTCAAAACACACACCCATGAAGCATAGTCTCCAACTAACTAATCCTTACCTTGTCCTCTCCTTTGCAACCTGTCTTCTTCATGTTCGTAAAAGCATTGGGGTCTTGTCTTCTCATGATCAAATGCCATTGCCATCGACATTTTAAAATTATTTCTGCTTTGATTATTCTTCCAACTTCGAACTTCCAACAACTGTGACAAACTACTCACTGTTTTGTCTATATCAAGATATGAGTATTGCTGTTGAAGGTTGATTGTACAGTATGTAGCAACAGCAACTAGTATTCTGCTTCATGTTCGTAACCAGGAAGGTTTTGCTTGTGCAGCTTCCTAGTTGGTGAAGGTGCTTGTACATGGGCAAAGACCAAAGGCATTTGTTTGCCAGAAACTATTGCAGAAGCTAATGAGGTTATATGAGTTACTGAGTCATATCTTGTGTGATTAATCTAAGCATTCTATTTAAAACTCTATAATTATTAACTCATCGATCTCTGACATTGTCGTGTGAGTCATCTGATATTTTGCCTACACATATAGTGGTTGGTGACAGAAAGGGCAAAAGCTCAATGGAAAAGATACAAGTCAATGGTTGAACGTGCAAAAAAAGAGAAAGGAACTACTAATGTAGTACATTCTTCTAGTCCTCAAGATAATGCTCGAATATCAGGTTTAGCCATCTATTTTATTTGTGATGGTTTAATAAATTTATCCTATTTTATCCATCCTTTTCACTTACTCCTACTAACTTTTTTGGTTTTGATTGTGTAGAACTCCAATCACACAATCCACCGGAAGGCACCACATGCCAGTCATCAACACGAAGCAACGAAGAAGAAGATTGTACTATGGACACAGTGGGTGTTATATGTGTTGACAGTGAAGGACATGTTGCATCTGGTGCCTCAAGTGGTGGTATTGCAATGAAGGTAGTTGTTATCAGCCTTGCCCTTACTACTTAGAAATGTATTTTCTTGATTATATAAGAATAATTTTAACTATTAGGTCAGTGGACGTGTGGGATTAGCAGCAATGTACGGTTCAGGCTGTTGGGCTTCCTCAAAAGGTCCATTTGGGGCTCCCTTCATGGTCGGGGGTTGTGTTTCCGGTGGAGGAGAGTATCTCATGAAAGGATTTGCAGCTAGGGAATGCTGTGTATCATTATCACTGTATGTTTGTCCACCACTCATGATTAAGTTCAGTTTATGTTAAATTCCACTATTTAATGAGAAAAATATATGGTTGATAAGATAAAATGTCCATCTGAATATCATTCCGTTCGTTGCCTTTTTTTTTATTTATTAGGGTCTTACTGTTTCCACATGAAACATTTTAAATATGTGCTGACTAGTTAACCTTTGTAAAGTCTGTTATTTTTGTTTGACATTGCTATATACGCTTCACTCAGATCACAGGCAGGTCCCGCCTCTGCTTGCATGAAAGTTCTACGCAATTTTGTTCAAGAAAGCAGTCAACATAATGCAGATAAGAGTGCTGGGATTTTACTTGTCCAGGCCGATGCTTCTAAATTGGTGACCTACTGAATGAACACACTTCTTGTCATTTCATTTAACAATGATTATGTATCTAAGTGTTGTTGCCACAGGCCCCAGGAAATCAACAGAAGTTGAAAGCTGTGGAGATTGCTGCTGCTTATTCTTCCCTTTCGTTTGGAATGGGCTACTTTGCAAGCTCTATGGATCGACCCAAGGTATTTATTAAGACTGTCTGATTCCGTGATCATGTCAACTTGTTTAAGAGCCAAAAGTCCTTTAAGTTACAAAATCCATCCCTTGGAAGAATAGAGAAGTTCTAGAGAGAGAGAGAGAGAGAGAGAAGGGTTAGAAGAAGCAGAGAACATTTTAGAGAGAACTTAGCGAGAGATATGTTAGAGAAAGGTAGATGGAGACAAAGATATGTTAGAGAAAAAGGGTACTCTGTGAGGTAAAAACGAGGTTTTGGAAGACATACACTTCAGTCCAAAATATAGAAACTAAAACAATCGTTATCATCCGTCCCGAAATTCCTATTCTTTTGTAGTGTTGAGATTATTCTGCCTCAAACAATCATAAAACTTCGGTTGATATGAGGCTCTCTATTTTCTTGTCTTAGGTGTCCATTTTGCGGAGTACAAAGCAAAACAACAAGCTGGGAATTGATCATTTTGAAGCACGTATAGATCTTTCAGACAATGGGAAGCTCTAATAAAACCCAGAAAGGAAATTCAAGAATTAACCTAATGCTCTCTCTTTCTCCATCAGGATTCAATTCCACAGAGAAAAAAAGGTCACAATTGTTAGAACTTTCATGCTGTTTTACTTAT

mRNA sequence

ATGAAGAGTGTACTAAAGAGGAAACTGAAGGGTGGGAGATGGAATTTTCGTCGCTTCATAAACGTGCAGTGCCGGCGTTTGGATCTCCGATTTCAACTACGCCTGCCTTTCCCGCCGGAACTCCTTCTTCCACCTTACACACTCTTTGATCGTCAGAGAATGGCCGCCGAAGCTCAAGACGAAGCTCATAGATTCTTCGTTGCAGTTCATGTTGGCGCGGGATTTCATGCTCCTTCCAACGAGAAAGCCCTCAGATCAGCCATGAAACGCGCTTGTTTAGCTGCCGCTGTAGTTCTTCGAAAGGGTTCAGGCGGCTGTTTAGATGCTGTTTCCGCTGCTATAAAAGTTCTAGAGGATGATCCAGCGACAAATGCTGGACGAGGCTCAAATTTGACGGATGATGGTCAAGTAGAATGTGATGCTAGTATTATGGATGGTGATACAGGTGCTTTTGGAGCTGTTGGTGCAGTGCCAGGTGTCCGAAATGCTATCCAAATTGCTACTTTACTAGCAAAGGAACAAATACTGGGATCATCTCTGCTGGGCCTTATACCTCCGATCTTCCTAGTTGGTGAAGGTGCTTGTACATGGGCAAAGACCAAAGGCATTTGTTTGCCAGAAACTATTGCAGAAGCTAATGAGTGGTTGGTGACAGAAAGGGCAAAAGCTCAATGGAAAAGATACAAGTCAATGGTTGAACGTGCAAAAAAAGAGAAAGGAACTACTAATGTAGTACATTCTTCTAGTCCTCAAGATAATGCTCGAATATCAGAACTCCAATCACACAATCCACCGGAAGGCACCACATGCCAGTCATCAACACGAAGCAACGAAGAAGAAGATTGTACTATGGACACAGTGGGTGTTATATGTGTTGACAGTGAAGGACATGTTGCATCTGGTGCCTCAAGTGGTGGTATTGCAATGAAGGTCAGTGGACGTGTGGGATTAGCAGCAATGTACGGTTCAGGCTGTTGGGCTTCCTCAAAAGGTCCATTTGGGGCTCCCTTCATGGTCGGGGGTTGTGTTTCCGGTGGAGGAGAGTATCTCATGAAAGGATTTGCAGCTAGGGAATGCTGTGTATCATTATCACTATCACAGGCAGGTCCCGCCTCTGCTTGCATGAAAGTTCTACGCAATTTTGTTCAAGAAAGCAGTCAACATAATGCAGATAAGAGTGCTGGGATTTTACTTGTCCAGGCCGATGCTTCTAAATTGGCCCCAGGAAATCAACAGAAGTTGAAAGCTGTGGAGATTGCTGCTGCTTATTCTTCCCTTTCGTTTGGAATGGGCTACTTTGCAAGCTCTATGGATCGACCCAAGGTGTCCATTTTGCGGAGTACAAAGCAAAACAACAAGCTGGGAATTGATCATTTTGAAGCACGTATAGATCTTTCAGACAATGGGAAGCTCTAATAAAACCCAGAAAGGAAATTCAAGAATTAACCTAATGCTCTCTCTTTCTCCATCAGGATTCAATTCCACAGAGAAAAAAAGGTCACAATTGTTAGAACTTTCATGCTGTTTTACTTAT

Coding sequence (CDS)

ATGAAGAGTGTACTAAAGAGGAAACTGAAGGGTGGGAGATGGAATTTTCGTCGCTTCATAAACGTGCAGTGCCGGCGTTTGGATCTCCGATTTCAACTACGCCTGCCTTTCCCGCCGGAACTCCTTCTTCCACCTTACACACTCTTTGATCGTCAGAGAATGGCCGCCGAAGCTCAAGACGAAGCTCATAGATTCTTCGTTGCAGTTCATGTTGGCGCGGGATTTCATGCTCCTTCCAACGAGAAAGCCCTCAGATCAGCCATGAAACGCGCTTGTTTAGCTGCCGCTGTAGTTCTTCGAAAGGGTTCAGGCGGCTGTTTAGATGCTGTTTCCGCTGCTATAAAAGTTCTAGAGGATGATCCAGCGACAAATGCTGGACGAGGCTCAAATTTGACGGATGATGGTCAAGTAGAATGTGATGCTAGTATTATGGATGGTGATACAGGTGCTTTTGGAGCTGTTGGTGCAGTGCCAGGTGTCCGAAATGCTATCCAAATTGCTACTTTACTAGCAAAGGAACAAATACTGGGATCATCTCTGCTGGGCCTTATACCTCCGATCTTCCTAGTTGGTGAAGGTGCTTGTACATGGGCAAAGACCAAAGGCATTTGTTTGCCAGAAACTATTGCAGAAGCTAATGAGTGGTTGGTGACAGAAAGGGCAAAAGCTCAATGGAAAAGATACAAGTCAATGGTTGAACGTGCAAAAAAAGAGAAAGGAACTACTAATGTAGTACATTCTTCTAGTCCTCAAGATAATGCTCGAATATCAGAACTCCAATCACACAATCCACCGGAAGGCACCACATGCCAGTCATCAACACGAAGCAACGAAGAAGAAGATTGTACTATGGACACAGTGGGTGTTATATGTGTTGACAGTGAAGGACATGTTGCATCTGGTGCCTCAAGTGGTGGTATTGCAATGAAGGTCAGTGGACGTGTGGGATTAGCAGCAATGTACGGTTCAGGCTGTTGGGCTTCCTCAAAAGGTCCATTTGGGGCTCCCTTCATGGTCGGGGGTTGTGTTTCCGGTGGAGGAGAGTATCTCATGAAAGGATTTGCAGCTAGGGAATGCTGTGTATCATTATCACTATCACAGGCAGGTCCCGCCTCTGCTTGCATGAAAGTTCTACGCAATTTTGTTCAAGAAAGCAGTCAACATAATGCAGATAAGAGTGCTGGGATTTTACTTGTCCAGGCCGATGCTTCTAAATTGGCCCCAGGAAATCAACAGAAGTTGAAAGCTGTGGAGATTGCTGCTGCTTATTCTTCCCTTTCGTTTGGAATGGGCTACTTTGCAAGCTCTATGGATCGACCCAAGGTGTCCATTTTGCGGAGTACAAAGCAAAACAACAAGCTGGGAATTGATCATTTTGAAGCACGTATAGATCTTTCAGACAATGGGAAGCTCTAA

Protein sequence

MKSVLKRKLKGGRWNFRRFINVQCRRLDLRFQLRLPFPPELLLPPYTLFDRQRMAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAAIKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKEQILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVERAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKGFAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGNQQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGKL*

Homology

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BLAST of CSPI03G08990.1 vs. ExPASy Swiss-Prot
Match: O65268 (Putative threonine aspartase OS=Arabidopsis thaliana OX=3702 GN=At4g00590 PE=2 SV=3)

HSP 1 Score: 515.8 bits (1327), Expect = 5.3e-145
Identity = 272/416 (65.38%), Postives = 339/416 (81.49%), Query Frame = 0


Query: 54  MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 113
           MA +  D++ RFFVAVHVGAG+HA +NEKALRS M+RACLAA+ +LR+ SG C+DAVSAA
Sbjct: 2   MAGDGADQSRRFFVAVHVGAGYHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAA 61

Query: 114 IKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE 173
           I+VLEDDP+TNAGRGSNLT+DG VECDAS+MDGD+G FG VGAVPGVRNAI+IA LL KE
Sbjct: 62  IQVLEDDPSTNAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKE 121

Query: 174 QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE 233
           QI GS+LLG IPP+ LVGEGA  W K+K + +P T+ EA++WLVTERA+ QW+R+K+M+ 
Sbjct: 122 QITGSTLLGRIPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAML- 181

Query: 234 RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRS--NEEEDCTMDTVGVIC 293
               E G  +++   S +++ R +E          TC+ +  S    +ED  MDTVGVIC
Sbjct: 182 ---SEVGAKSIL---SAEEHPRGTE-------NNETCEENVSSCAAADEDKIMDTVGVIC 241

Query: 294 VDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLM 353
           VD+EGH+A G+SSGGIAMK+SGRVGLAA YGSGCWASSKGPFGAPF+VG CVSG GEYLM
Sbjct: 242 VDNEGHIACGSSSGGIAMKISGRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLM 301

Query: 354 KGFAARECCVSLSLSQAGPASACMKVLRNFV-QESSQ-HNADKSAGILLVQADASKLAPG 413
           +GFAARECC SL+LSQAGPASA MKVLR+ + QESS+   ADK+AGIL+VQADAS + PG
Sbjct: 302 RGFAARECCTSLALSQAGPASAAMKVLRSVMHQESSKIGTADKTAGILVVQADASVVVPG 361

Query: 414 NQQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDL 466
           ++ +L AVEIAAAYSSLSFG+GY+ +S+++PK+SILR+ +Q ++ G+DHFEARIDL
Sbjct: 362 SKPELNAVEIAAAYSSLSFGVGYYGNSIEKPKISILRTRRQMSEAGVDHFEARIDL 403

[Close]

BLAST of CSPI03G08990.1 vs. ExPASy Swiss-Prot
Match: Q9H6P5 (Threonine aspartase 1 OS=Homo sapiens OX=9606 GN=TASP1 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.6e-45
Identity = 142/387 (36.69%), Postives = 203/387 (52.45%), Query Frame = 0


Query: 66  FVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAAIKVLEDDPATNA 125
           FV VH GAG+H+ S  K  +   KRAC  A   L+ G+    DAV+AA+  LED P TNA
Sbjct: 43  FVLVHAGAGYHSESKAKEYKHVCKRACQKAIEKLQAGA-LATDAVTAALVELEDSPFTNA 102

Query: 126 GRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKEQILGSSLLGLIP 185
           G GSNL   G++ECDASIMDG +  FGAVGA+ G++N + +A  L  E   G    G IP
Sbjct: 103 GMGSNLNLLGEIECDASIMDGKSLNFGAVGALSGIKNPVSVANRLLCEGQKGKLSAGRIP 162

Query: 186 PIFLVGEGACTWAKTKGI--CLPETIAEANEWLVTERAKAQWKRYKSMVERAKKEKGTTN 245
           P FLVGEGA  WA   GI  C P         + T  + A +KR K  +E A++      
Sbjct: 163 PCFLVGEGAYRWAVDHGIPSCPPNI-------MTTRFSLAAFKRNKRKLELAERV----- 222

Query: 246 VVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVDSEGHVASGAS 305
                    +    +L+          QSS + N+    T+DTVG + VD EG+VA+  S
Sbjct: 223 ---------DTDFMQLKKRR-------QSSEKENDSG--TLDTVGAVVVDHEGNVAAAVS 282

Query: 306 SGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKGFAARECCVSL 365
           SGG+A+K  GRVG AA+YG GCWA + G    P+      SG GE+L++   AREC  +L
Sbjct: 283 SGGLALKHPGRVGQAALYGCGCWAENTGAHN-PYSTAVSTSGCGEHLVRTILARECSHAL 342

Query: 366 SLSQAGPASACMKVLRN-FVQESSQHNAD-KSAGILLVQADASKLAPGNQQKLKA--VEI 425
               A    A ++ ++N F+      + D    G++++++      P + Q  +   VE 
Sbjct: 343 QAEDA--HQALLETMQNKFISSPFLASEDGVLGGVIVLRSCRCSAEPDSSQNKQTLLVEF 395

Query: 426 AAAYSSLSFGMGYFASSMDRPKVSILR 447
             ++++ S  +GY ++   + K  I R
Sbjct: 403 LWSHTTESMCVGYMSAQDGKAKTHISR 395

[Close]

BLAST of CSPI03G08990.1 vs. ExPASy Swiss-Prot
Match: Q8R1G1 (Threonine aspartase 1 OS=Mus musculus OX=10090 GN=Tasp1 PE=2 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.8e-45
Identity = 147/387 (37.98%), Postives = 201/387 (51.94%), Query Frame = 0


Query: 66  FVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAAIKVLEDDPATNA 125
           FV VH GAG+H+ S  K  +   KRAC  A   L+ G+    DAV+AA+  LED P TNA
Sbjct: 43  FVLVHAGAGYHSESKAKEYKHVCKRACQKAIEKLQAGA-LATDAVAAALVELEDSPFTNA 102

Query: 126 GRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKEQILGSSLLGLIP 185
           G GSNL   G++ECDASIMDG +  FGAVGA+ G++N + +A  L  E   G    G IP
Sbjct: 103 GIGSNLNLLGEIECDASIMDGKSLNFGAVGALSGIKNPVSVAHRLLCEGQKGKLSAGRIP 162

Query: 186 PIFLVGEGACTWAKTKGI--CLPETIAEANEWLVTERAKAQWKRYKSMVERAKKEKGTTN 245
           P FLVGEGA  WA   GI  C P T       + T  + A +KR K  +E A  E+  T+
Sbjct: 163 PCFLVGEGAYRWAVDHGIPSCPPST-------MTTRFSLAAFKRNKRKLELA--ERVETD 222

Query: 246 VVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVDSEGHVASGAS 305
            +     +                   QSS + N+    T+DTVG + VD EG+VA+  S
Sbjct: 223 FIQLKRRR-------------------QSSAKENDSG--TLDTVGAVVVDHEGNVAAAVS 282

Query: 306 SGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKGFAARECCVSL 365
           SGG+A+K  GRVG AA+YG GCWA + G    P+      SG GE+L++   AREC  +L
Sbjct: 283 SGGLALKHPGRVGQAALYGCGCWAENTGAQN-PYSTAVSTSGCGEHLVRTILARECSHAL 342

Query: 366 SLSQAGPASACMKVLRN-FVQESSQHNADKSAG--ILLVQADASKLAPGNQQK-LKAVEI 425
               A    A ++ ++N F+        D   G  I+L     S  +  +Q K    VE 
Sbjct: 343 QAEDA--HQALLETMQNKFISSPFLACEDGVLGGVIVLRSCRCSSESDSSQDKQTLLVEF 395

Query: 426 AAAYSSLSFGMGYFASSMDRPKVSILR 447
             ++++ S  +GY ++   + K  I R
Sbjct: 403 LWSHTTESMCVGYMSAQDGKAKTHISR 395

[Close]

BLAST of CSPI03G08990.1 vs. ExPASy Swiss-Prot
Match: Q9P6N7 (Putative threonine aspartase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC823.09c PE=3 SV=3)

HSP 1 Score: 105.9 bits (263), Expect = 1.3e-21
Identity = 104/391 (26.60%), Postives = 168/391 (42.97%), Query Frame = 0


Query: 67  VAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAAIKVLEDDPATNAG 126
           V +H GAG ++   E   +     AC AA   L+ G      A+ AA K++ED P TNAG
Sbjct: 6   VILHAGAGLYSGQREIQAKKTCSDACKAAIQALKVGQSALSAAIQAA-KIMEDSPVTNAG 65

Query: 127 RGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIAT-LLAKEQILGSSLLGLIP 186
            GSNL  DG+VEC+A +MD ++G   +V      R+  +    +L K +++  S  GL+P
Sbjct: 66  VGSNLNIDGKVECEAGVMDSESGLTASVACCNCCRHPSEACLYILNKRKVM--SQHGLVP 125

Query: 187 PIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVERAKKEKGTTNVV 246
           P  LVG G           + + +  +N  LV E                      ++++
Sbjct: 126 PAMLVGNG-----------IEKLLLHSNIKLVPE----------------------SHLI 185

Query: 247 HSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVDSEGHVASGASSG 306
              S +   +  E+   NP   +              + DT+GVICVD  G +A  +SSG
Sbjct: 186 TERSMKTQIKWKEILYQNPINLS--------------SQDTIGVICVDKNGRIAVVSSSG 245

Query: 307 GIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKG-FAARECCVSLS 366
           G+ +K +GR+G + + G G W  S              SG GE++    FA R     L 
Sbjct: 246 GLLLKPAGRIGSSPIPGHGFWIESFDNKSHSSTCAVATSGTGEHISNTCFACRSS--QLL 305

Query: 367 LSQAGPASACMKVLRNFVQE-SSQHNADKSAGILLVQADASKLAPGNQQKLKAVEIAAAY 426
           +S+    S+  K++ +F +  S+   +D   GI+  + + S          K +    A+
Sbjct: 306 VSEDNVVSSLNKLINDFHEHPSATLYSDLQVGIIFAKVETS------NSHNKRIIFGLAH 338

Query: 427 SSLSFGMGYFASSMDRPKVSILRSTKQNNKL 455
           SS     G+      +P   I R   + + +
Sbjct: 366 SSPDMVFGFMKGDHSKPTTEISRKGSKRSSV 338

[Close]

BLAST of CSPI03G08990.1 vs. ExPASy Swiss-Prot
Match: Q6GM78 (Isoaspartyl peptidase/L-asparaginase OS=Xenopus laevis OX=8355 GN=asrgl1 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.2e-19
Identity = 95/292 (32.53%), Postives = 133/292 (45.55%), Query Frame = 0


Query: 67  VAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAAIKVLEDDPATNAG 126
           + VH GAG      +   R+ +KRA L    VL +G G  L AV  A+ VLED+   NAG
Sbjct: 5   IVVHGGAGKIVEELDATYRAGVKRAVLKGYDVLSQG-GSALTAVEEAVIVLEDEQIFNAG 64

Query: 127 RGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKEQILGSSLLGLIPP 186
            GS L + G +E DA IMDG     GAV A+  + N I++A L+ ++             
Sbjct: 65  HGSVLNEKGDIEMDAIIMDGKNLDSGAVSAIRNIANPIKLARLVMEK----------TDH 124

Query: 187 IFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVERAKKEKGTTNVVH 246
           + L  EGA  +AK +GI  PE     NE LVTER++ +W +               N+  
Sbjct: 125 MLLTCEGATLFAKAQGI--PEV---PNESLVTERSRKRWMK---------------NLKE 184

Query: 247 SSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVDSEGHVASGASSGG 306
           +S+P                             +   + TVG + +D EG+VA   S+GG
Sbjct: 185 NSNP---------------------------VADQIGLGTVGAVAIDCEGNVACATSTGG 230

Query: 307 IAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGG-CVSGGGEYLMKGFAAR 358
           +  K+ GRVG  A  GSG +A +         VG    +G GE +MK   AR
Sbjct: 245 LTNKMVGRVGDTACIGSGGYADNN--------VGAVSTTGHGESIMKVILAR 230

[Close]

BLAST of CSPI03G08990.1 vs. ExPASy TrEMBL
Match: A0A0A0L7I2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G122610 PE=4 SV=1)

HSP 1 Score: 909.4 bits (2349), Expect = 6.0e-261
Identity = 469/471 (99.58%), Postives = 469/471 (99.58%), Query Frame = 0


Query: 1   MKSVLKRKLKGGRWNFRRFINVQCRRLDLRFQLRLPFPPELLLPPYTLFDRQRMAAEAQD 60
           MKSVLKRKLKGGRWNFRRFINVQCRRL LRFQLRLPFPPELLLPPY LFDRQRMAAEAQD
Sbjct: 1   MKSVLKRKLKGGRWNFRRFINVQCRRLALRFQLRLPFPPELLLPPYILFDRQRMAAEAQD 60

Query: 61  EAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAAIKVLEDD 120
           EAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAAIKVLEDD
Sbjct: 61  EAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAAIKVLEDD 120

Query: 121 PATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKEQILGSSL 180
           PATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKEQILGSSL
Sbjct: 121 PATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKEQILGSSL 180

Query: 181 LGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVERAKKEKG 240
           LGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVERAKKEKG
Sbjct: 181 LGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVERAKKEKG 240

Query: 241 TTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVDSEGHVAS 300
           TTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVDSEGHVAS
Sbjct: 241 TTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVDSEGHVAS 300

Query: 301 GASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKGFAARECC 360
           GASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKGFAARECC
Sbjct: 301 GASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKGFAARECC 360

Query: 361 VSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGNQQKLKAVEIA 420
           VSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGNQQKLKAVEIA
Sbjct: 361 VSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGNQQKLKAVEIA 420

Query: 421 AAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGKL 472
           AAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGKL
Sbjct: 421 AAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGKL 471

[Close]

BLAST of CSPI03G08990.1 vs. ExPASy TrEMBL
Match: A0A5D3D030 (Putative threonine aspartase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G004510 PE=4 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 8.8e-220
Identity = 403/421 (95.72%), Postives = 408/421 (96.91%), Query Frame = 0


Query: 54  MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 113
           MAAEAQ+EAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA
Sbjct: 1   MAAEAQEEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 60

Query: 114 IKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE 173
           IKVLEDDPATNAGRGSNLT+DGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIA LLAKE
Sbjct: 61  IKVLEDDPATNAGRGSNLTEDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIAALLAKE 120

Query: 174 QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE 233
           QILGSSLLGLIPPIFLVGEGA TWAKTKGICLPETIAEANEWLVTERAK QWKRYKSMVE
Sbjct: 121 QILGSSLLGLIPPIFLVGEGARTWAKTKGICLPETIAEANEWLVTERAKVQWKRYKSMVE 180

Query: 234 RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSN---EEEDCTMDTVGVI 293
           RAKKEKGT+NV HS  PQDNARISE+QSHNPPEGTTCQSSTRSN   EEEDCTMDTVGVI
Sbjct: 181 RAKKEKGTSNVAHSCCPQDNARISEVQSHNPPEGTTCQSSTRSNEEEEEEDCTMDTVGVI 240

Query: 294 CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL 353
           CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL
Sbjct: 241 CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL 300

Query: 354 MKGFAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGN 413
           MKGFAARECCVSLSLSQAGP SACMKVLRNFVQESSQHNADKSAGILLVQADASK APGN
Sbjct: 301 MKGFAARECCVSLSLSQAGPGSACMKVLRNFVQESSQHNADKSAGILLVQADASKSAPGN 360

Query: 414 QQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGK 472
            QKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNK+GIDHFEARIDLSDN K
Sbjct: 361 PQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKMGIDHFEARIDLSDNRK 420

[Close]

BLAST of CSPI03G08990.1 vs. ExPASy TrEMBL
Match: A0A1S3AVH3 (putative threonine aspartase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483292 PE=4 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 8.8e-220
Identity = 403/421 (95.72%), Postives = 408/421 (96.91%), Query Frame = 0


Query: 54  MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 113
           MAAEAQ+EAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA
Sbjct: 1   MAAEAQEEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 60

Query: 114 IKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE 173
           IKVLEDDPATNAGRGSNLT+DGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIA LLAKE
Sbjct: 61  IKVLEDDPATNAGRGSNLTEDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIAALLAKE 120

Query: 174 QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE 233
           QILGSSLLGLIPPIFLVGEGA TWAKTKGICLPETIAEANEWLVTERAK QWKRYKSMVE
Sbjct: 121 QILGSSLLGLIPPIFLVGEGARTWAKTKGICLPETIAEANEWLVTERAKVQWKRYKSMVE 180

Query: 234 RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSN---EEEDCTMDTVGVI 293
           RAKKEKGT+NV HS  PQDNARISE+QSHNPPEGTTCQSSTRSN   EEEDCTMDTVGVI
Sbjct: 181 RAKKEKGTSNVAHSCCPQDNARISEVQSHNPPEGTTCQSSTRSNEEEEEEDCTMDTVGVI 240

Query: 294 CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL 353
           CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL
Sbjct: 241 CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL 300

Query: 354 MKGFAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGN 413
           MKGFAARECCVSLSLSQAGP SACMKVLRNFVQESSQHNADKSAGILLVQADASK APGN
Sbjct: 301 MKGFAARECCVSLSLSQAGPGSACMKVLRNFVQESSQHNADKSAGILLVQADASKSAPGN 360

Query: 414 QQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGK 472
            QKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNK+GIDHFEARIDLSDN K
Sbjct: 361 PQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKMGIDHFEARIDLSDNRK 420

[Close]

BLAST of CSPI03G08990.1 vs. ExPASy TrEMBL
Match: A0A1S4DSV0 (putative threonine aspartase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483292 PE=4 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 6.3e-218
Identity = 402/421 (95.49%), Postives = 407/421 (96.67%), Query Frame = 0


Query: 54  MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 113
           MAAEAQ+EAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA
Sbjct: 1   MAAEAQEEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 60

Query: 114 IKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE 173
           IKVLEDDPATNAGRGSNLT+DGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIA LLAKE
Sbjct: 61  IKVLEDDPATNAGRGSNLTEDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIAALLAKE 120

Query: 174 QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE 233
           QILGSSLLGLIPPIFLVGEGA TWAKTKGICLPETIAEANEWLVTERAK QWKRYKSMVE
Sbjct: 121 QILGSSLLGLIPPIFLVGEGARTWAKTKGICLPETIAEANEWLVTERAKVQWKRYKSMVE 180

Query: 234 RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSN---EEEDCTMDTVGVI 293
           RAKKEKGT+NV HS  PQDNARIS +QSHNPPEGTTCQSSTRSN   EEEDCTMDTVGVI
Sbjct: 181 RAKKEKGTSNVAHSCCPQDNARIS-VQSHNPPEGTTCQSSTRSNEEEEEEDCTMDTVGVI 240

Query: 294 CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL 353
           CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL
Sbjct: 241 CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL 300

Query: 354 MKGFAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGN 413
           MKGFAARECCVSLSLSQAGP SACMKVLRNFVQESSQHNADKSAGILLVQADASK APGN
Sbjct: 301 MKGFAARECCVSLSLSQAGPGSACMKVLRNFVQESSQHNADKSAGILLVQADASKSAPGN 360

Query: 414 QQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGK 472
            QKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNK+GIDHFEARIDLSDN K
Sbjct: 361 PQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKMGIDHFEARIDLSDNRK 420

[Close]

BLAST of CSPI03G08990.1 vs. ExPASy TrEMBL
Match: A0A1S3AW50 (putative threonine aspartase isoform X3 OS=Cucumis melo OX=3656 GN=LOC103483292 PE=4 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 3.8e-207
Identity = 386/421 (91.69%), Postives = 391/421 (92.87%), Query Frame = 0


Query: 54  MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 113
           MAAEAQ+EAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRK            
Sbjct: 1   MAAEAQEEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRK------------ 60

Query: 114 IKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE 173
                DDPATNAGRGSNLT+DGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIA LLAKE
Sbjct: 61  -----DDPATNAGRGSNLTEDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIAALLAKE 120

Query: 174 QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE 233
           QILGSSLLGLIPPIFLVGEGA TWAKTKGICLPETIAEANEWLVTERAK QWKRYKSMVE
Sbjct: 121 QILGSSLLGLIPPIFLVGEGARTWAKTKGICLPETIAEANEWLVTERAKVQWKRYKSMVE 180

Query: 234 RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSN---EEEDCTMDTVGVI 293
           RAKKEKGT+NV HS  PQDNARISE+QSHNPPEGTTCQSSTRSN   EEEDCTMDTVGVI
Sbjct: 181 RAKKEKGTSNVAHSCCPQDNARISEVQSHNPPEGTTCQSSTRSNEEEEEEDCTMDTVGVI 240

Query: 294 CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL 353
           CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL
Sbjct: 241 CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL 300

Query: 354 MKGFAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGN 413
           MKGFAARECCVSLSLSQAGP SACMKVLRNFVQESSQHNADKSAGILLVQADASK APGN
Sbjct: 301 MKGFAARECCVSLSLSQAGPGSACMKVLRNFVQESSQHNADKSAGILLVQADASKSAPGN 360

Query: 414 QQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGK 472
            QKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNK+GIDHFEARIDLSDN K
Sbjct: 361 PQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKMGIDHFEARIDLSDNRK 404

[Close]

BLAST of CSPI03G08990.1 vs. NCBI nr
Match: XP_011650761.2 (putative threonine aspartase isoform X1 [Cucumis sativus])

HSP 1 Score: 909.4 bits (2349), Expect = 1.2e-260
Identity = 469/471 (99.58%), Postives = 469/471 (99.58%), Query Frame = 0


Query: 1   MKSVLKRKLKGGRWNFRRFINVQCRRLDLRFQLRLPFPPELLLPPYTLFDRQRMAAEAQD 60
           MKSVLKRKLKGGRWNFRRFINVQCRRL LRFQLRLPFPPELLLPPY LFDRQRMAAEAQD
Sbjct: 1   MKSVLKRKLKGGRWNFRRFINVQCRRLALRFQLRLPFPPELLLPPYILFDRQRMAAEAQD 60

Query: 61  EAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAAIKVLEDD 120
           EAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAAIKVLEDD
Sbjct: 61  EAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAAIKVLEDD 120

Query: 121 PATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKEQILGSSL 180
           PATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKEQILGSSL
Sbjct: 121 PATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKEQILGSSL 180

Query: 181 LGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVERAKKEKG 240
           LGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVERAKKEKG
Sbjct: 181 LGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVERAKKEKG 240

Query: 241 TTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVDSEGHVAS 300
           TTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVDSEGHVAS
Sbjct: 241 TTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVDSEGHVAS 300

Query: 301 GASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKGFAARECC 360
           GASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKGFAARECC
Sbjct: 301 GASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKGFAARECC 360

Query: 361 VSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGNQQKLKAVEIA 420
           VSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGNQQKLKAVEIA
Sbjct: 361 VSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGNQQKLKAVEIA 420

Query: 421 AAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGKL 472
           AAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGKL
Sbjct: 421 AAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGKL 471

[Close]

BLAST of CSPI03G08990.1 vs. NCBI nr
Match: KGN56576.2 (hypothetical protein Csa_011804 [Cucumis sativus])

HSP 1 Score: 807.4 bits (2084), Expect = 6.7e-230
Identity = 418/418 (100.00%), Postives = 418/418 (100.00%), Query Frame = 0


Query: 54  MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 113
           MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA
Sbjct: 1   MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 60

Query: 114 IKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE 173
           IKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE
Sbjct: 61  IKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE 120

Query: 174 QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE 233
           QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE
Sbjct: 121 QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE 180

Query: 234 RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVD 293
           RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVD
Sbjct: 181 RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVD 240

Query: 294 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKG 353
           SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKG
Sbjct: 241 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKG 300

Query: 354 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGNQQK 413
           FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGNQQK
Sbjct: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGNQQK 360

Query: 414 LKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGKL 472
           LKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGKL
Sbjct: 361 LKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGKL 418

[Close]

BLAST of CSPI03G08990.1 vs. NCBI nr
Match: XP_008438074.1 (PREDICTED: putative threonine aspartase isoform X1 [Cucumis melo] >KAA0048983.1 putative threonine aspartase isoform X1 [Cucumis melo var. makuwa] >TYK17581.1 putative threonine aspartase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 772.7 bits (1994), Expect = 1.8e-219
Identity = 403/421 (95.72%), Postives = 408/421 (96.91%), Query Frame = 0


Query: 54  MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 113
           MAAEAQ+EAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA
Sbjct: 1   MAAEAQEEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 60

Query: 114 IKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE 173
           IKVLEDDPATNAGRGSNLT+DGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIA LLAKE
Sbjct: 61  IKVLEDDPATNAGRGSNLTEDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIAALLAKE 120

Query: 174 QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE 233
           QILGSSLLGLIPPIFLVGEGA TWAKTKGICLPETIAEANEWLVTERAK QWKRYKSMVE
Sbjct: 121 QILGSSLLGLIPPIFLVGEGARTWAKTKGICLPETIAEANEWLVTERAKVQWKRYKSMVE 180

Query: 234 RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSN---EEEDCTMDTVGVI 293
           RAKKEKGT+NV HS  PQDNARISE+QSHNPPEGTTCQSSTRSN   EEEDCTMDTVGVI
Sbjct: 181 RAKKEKGTSNVAHSCCPQDNARISEVQSHNPPEGTTCQSSTRSNEEEEEEDCTMDTVGVI 240

Query: 294 CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL 353
           CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL
Sbjct: 241 CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL 300

Query: 354 MKGFAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGN 413
           MKGFAARECCVSLSLSQAGP SACMKVLRNFVQESSQHNADKSAGILLVQADASK APGN
Sbjct: 301 MKGFAARECCVSLSLSQAGPGSACMKVLRNFVQESSQHNADKSAGILLVQADASKSAPGN 360

Query: 414 QQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGK 472
            QKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNK+GIDHFEARIDLSDN K
Sbjct: 361 PQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKMGIDHFEARIDLSDNRK 420

[Close]

BLAST of CSPI03G08990.1 vs. NCBI nr
Match: XP_016899072.1 (PREDICTED: putative threonine aspartase isoform X2 [Cucumis melo])

HSP 1 Score: 766.5 bits (1978), Expect = 1.3e-217
Identity = 402/421 (95.49%), Postives = 407/421 (96.67%), Query Frame = 0


Query: 54  MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 113
           MAAEAQ+EAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA
Sbjct: 1   MAAEAQEEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 60

Query: 114 IKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE 173
           IKVLEDDPATNAGRGSNLT+DGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIA LLAKE
Sbjct: 61  IKVLEDDPATNAGRGSNLTEDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIAALLAKE 120

Query: 174 QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE 233
           QILGSSLLGLIPPIFLVGEGA TWAKTKGICLPETIAEANEWLVTERAK QWKRYKSMVE
Sbjct: 121 QILGSSLLGLIPPIFLVGEGARTWAKTKGICLPETIAEANEWLVTERAKVQWKRYKSMVE 180

Query: 234 RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSN---EEEDCTMDTVGVI 293
           RAKKEKGT+NV HS  PQDNARIS +QSHNPPEGTTCQSSTRSN   EEEDCTMDTVGVI
Sbjct: 181 RAKKEKGTSNVAHSCCPQDNARIS-VQSHNPPEGTTCQSSTRSNEEEEEEDCTMDTVGVI 240

Query: 294 CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL 353
           CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL
Sbjct: 241 CVDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYL 300

Query: 354 MKGFAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGN 413
           MKGFAARECCVSLSLSQAGP SACMKVLRNFVQESSQHNADKSAGILLVQADASK APGN
Sbjct: 301 MKGFAARECCVSLSLSQAGPGSACMKVLRNFVQESSQHNADKSAGILLVQADASKSAPGN 360

Query: 414 QQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGK 472
            QKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNK+GIDHFEARIDLSDN K
Sbjct: 361 PQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKMGIDHFEARIDLSDNRK 420

[Close]

BLAST of CSPI03G08990.1 vs. NCBI nr
Match: XP_011650762.1 (putative threonine aspartase isoform X2 [Cucumis sativus])

HSP 1 Score: 765.4 bits (1975), Expect = 2.9e-217
Identity = 401/418 (95.93%), Postives = 401/418 (95.93%), Query Frame = 0


Query: 54  MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 113
           MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRK            
Sbjct: 1   MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRK------------ 60

Query: 114 IKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE 173
                DDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE
Sbjct: 61  -----DDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE 120

Query: 174 QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE 233
           QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE
Sbjct: 121 QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE 180

Query: 234 RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVD 293
           RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVD
Sbjct: 181 RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEEDCTMDTVGVICVD 240

Query: 294 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKG 353
           SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKG
Sbjct: 241 SEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKG 300

Query: 354 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGNQQK 413
           FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGNQQK
Sbjct: 301 FAARECCVSLSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGNQQK 360

Query: 414 LKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGKL 472
           LKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGKL
Sbjct: 361 LKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDLSDNGKL 401

[Close]

BLAST of CSPI03G08990.1 vs. TAIR 10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 515.8 bits (1327), Expect = 3.7e-146
Identity = 272/416 (65.38%), Postives = 339/416 (81.49%), Query Frame = 0


Query: 54  MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 113
           MA +  D++ RFFVAVHVGAG+HA +NEKALRS M+RACLAA+ +LR+ SG C+DAVSAA
Sbjct: 2   MAGDGADQSRRFFVAVHVGAGYHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAA 61

Query: 114 IKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE 173
           I+VLEDDP+TNAGRGSNLT+DG VECDAS+MDGD+G FG VGAVPGVRNAI+IA LL KE
Sbjct: 62  IQVLEDDPSTNAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKE 121

Query: 174 QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE 233
           QI GS+LLG IPP+ LVGEGA  W K+K + +P T+ EA++WLVTERA+ QW+R+K+M+ 
Sbjct: 122 QITGSTLLGRIPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAML- 181

Query: 234 RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRS--NEEEDCTMDTVGVIC 293
               E G  +++   S +++ R +E          TC+ +  S    +ED  MDTVGVIC
Sbjct: 182 ---SEVGAKSIL---SAEEHPRGTE-------NNETCEENVSSCAAADEDKIMDTVGVIC 241

Query: 294 VDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLM 353
           VD+EGH+A G+SSGGIAMK+SGRVGLAA YGSGCWASSKGPFGAPF+VG CVSG GEYLM
Sbjct: 242 VDNEGHIACGSSSGGIAMKISGRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLM 301

Query: 354 KGFAARECCVSLSLSQAGPASACMKVLRNFV-QESSQ-HNADKSAGILLVQADASKLAPG 413
           +GFAARECC SL+LSQAGPASA MKVLR+ + QESS+   ADK+AGIL+VQADAS + PG
Sbjct: 302 RGFAARECCTSLALSQAGPASAAMKVLRSVMHQESSKIGTADKTAGILVVQADASVVVPG 361

Query: 414 NQQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDL 466
           ++ +L AVEIAAAYSSLSFG+GY+ +S+++PK+SILR+ +Q ++ G+DHFEARIDL
Sbjct: 362 SKPELNAVEIAAAYSSLSFGVGYYGNSIEKPKISILRTRRQMSEAGVDHFEARIDL 403

[Close]

BLAST of CSPI03G08990.1 vs. TAIR 10
Match: AT4G00590.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 466.8 bits (1200), Expect = 2.0e-131
Identity = 254/416 (61.06%), Postives = 323/416 (77.64%), Query Frame = 0


Query: 54  MAAEAQDEAHRFFVAVHVGAGFHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAA 113
           MA +  D++ RFFVAVHVGAG+HA +NEKALRS M+RACLAA+ +LR+ SG C+DAVSAA
Sbjct: 2   MAGDGADQSRRFFVAVHVGAGYHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAA 61

Query: 114 IKVLEDDPATNAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKE 173
           I+VLEDDP+TNAGRGSNLT+DG VECDAS+MDGD+G FG VGAVP      Q++ +L+K 
Sbjct: 62  IQVLEDDPSTNAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPD-----QVSEMLSK- 121

Query: 174 QILGSSLLGLIPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVE 233
                    L+    LVGEGA  W K+K + +P T+ EA++WLVTERA+ QW+R+K+M+ 
Sbjct: 122 ---------LLLCWLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAML- 181

Query: 234 RAKKEKGTTNVVHSSSPQDNARISELQSHNPPEGTTCQSSTRS--NEEEDCTMDTVGVIC 293
               E G  +++   S +++ R +E          TC+ +  S    +ED  MDTVGVIC
Sbjct: 182 ---SEVGAKSIL---SAEEHPRGTE-------NNETCEENVSSCAAADEDKIMDTVGVIC 241

Query: 294 VDSEGHVASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLM 353
           VD+EGH+A G+SSGGIAMK+SGRVGLAA YGSGCWASSKGPFGAPF+VG CVSG GEYLM
Sbjct: 242 VDNEGHIACGSSSGGIAMKISGRVGLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLM 301

Query: 354 KGFAARECCVSLSLSQAGPASACMKVLRNFV-QESSQ-HNADKSAGILLVQADASKLAPG 413
           +GFAARECC SL+LSQAGPASA MKVLR+ + QESS+   ADK+AGIL+VQADAS + PG
Sbjct: 302 RGFAARECCTSLALSQAGPASAAMKVLRSVMHQESSKIGTADKTAGILVVQADASVVVPG 361

Query: 414 NQQKLKAVEIAAAYSSLSFGMGYFASSMDRPKVSILRSTKQNNKLGIDHFEARIDL 466
           ++ +L AVEIAAAYSSLSFG+GY+ +S+++PK+SILR+ +Q ++ G+DHFEARIDL
Sbjct: 362 SKPELNAVEIAAAYSSLSFGVGYYGNSIEKPKISILRTRRQMSEAGVDHFEARIDL 388

[Close]

BLAST of CSPI03G08990.1 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 90.9 bits (224), Expect = 3.0e-18
Identity = 86/300 (28.67%), Postives = 138/300 (46.00%), Query Frame = 0


Query: 67  VAVHVGAGF--HAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAAIKVLEDDPATN 126
           +AVH GAG   + P+  +     +   CL   ++  + +   +D V   I+ LE DP  N
Sbjct: 6   IAVHGGAGIDPNLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELETDPLFN 65

Query: 127 AGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKEQILGSSLLGLI 186
           +GRGS LT+ G VE +ASIMDG     GAV  +  V+N I +A L+  +           
Sbjct: 66  SGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKS---------- 125

Query: 187 PPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVERAKKEKGTTNV 246
           P  +L   GA  +A+ +G+ + +     NE+ VT+      K  K               
Sbjct: 126 PHSYLAFSGAEDFARKQGVEIVD-----NEYFVTDDNVGMLKLAK--------------- 185

Query: 247 VHSSSPQDNARISELQSHNPPEGTTCQSSTRSNEEED------CTMDTVGVICVDSEGHV 306
             ++S   + RI       PP G    ++T S  + +         +TVG + VD +GH 
Sbjct: 186 -EANSILFDYRI-------PPMGCAGAAATDSPIQMNGLPISIYAPETVGCVVVDGKGHC 245

Query: 307 ASGASSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKGFAARE 359
           A+G S+GG+  K+ GR+G + + G+G +AS           G   +G GE +++   AR+
Sbjct: 246 AAGTSTGGLMNKMMGRIGDSPLIGAGTYASE--------FCGVSCTGEGEAIIRATLARD 259

[Close]

BLAST of CSPI03G08990.1 vs. TAIR 10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 8.1e-16
Identity = 92/348 (26.44%), Postives = 143/348 (41.09%), Query Frame = 0


Query: 67  VAVHVGAG---FHAPSNEKALRSAMKRACLAAAVVLRKGSGGCLDAVSAAIKVLEDDPAT 126
           +A+H GAG      P   +  R +  R CL   +   K     LD     ++ LE+ P  
Sbjct: 6   IALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELENHPDF 65

Query: 127 NAGRGSNLTDDGQVECDASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKEQILGSSLLGL 186
           NAG+GS LT  G VE +ASIMDG T   GAV  +  V N I +A L+ ++          
Sbjct: 66  NAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEK---------- 125

Query: 187 IPPIFLVGEGACTWAKTKGICLPETIAEANEWLVTERAKAQWKRYKSMVERAKKEKGTTN 246
            P I+L  + A  +A+  G+   ET+  +                               
Sbjct: 126 TPHIYLAFDAAEAFARAHGV---ETVDSS------------------------------- 185

Query: 247 VVHSSSPQDNARISELQSHNPPE-GTTCQSSTRSNEEEDCTMDTVGVICVDSEGHVASGA 306
             H  +P++ AR+ + +  N  +   T  S    +   D  + TVG + VDS G++AS  
Sbjct: 186 --HFITPENIARLKQAKEFNRVQLDYTVPSPKVPDNCGDSQIGTVGCVAVDSAGNLASAT 245

Query: 307 SSGGIAMKVSGRVGLAAMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKGFAARECCVS 366
           S+GG   K+ GR+G   + G+G +A+      A        +G GE +++G  AR+    
Sbjct: 246 STGGYVNKMVGRIGDTPVIGAGTYANHLCAISA--------TGKGEDIIRGTVARDVAAL 292

Query: 367 LSLSQAGPASACMKVLRNFVQESSQHNADKSAGILLVQADASKLAPGN 411
           +         A   V+   V          S G++ V A+     P N
Sbjct: 306 MEYKGLSLTEAAAYVVDQSVPRG-------SCGLVAVSANGEVTMPFN 292

[Close]

BLAST of CSPI03G08990.1 vs. TAIR 10
Match: AT5G08100.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 1.3e-05
Identity = 66/272 (24.26%), Postives = 108/272 (39.71%), Query Frame = 0


Query: 140 DASIMDGDTGAFGAVGAVPGVRNAIQIATLLAKEQILGSSLLGLIPPIFLVGEGACTWAK 199
           +ASIMDG T   GAV  +  V N I +A L+ ++           P I+L  + A  +A+
Sbjct: 2   EASIMDGKTKRCGAVSGLTTVVNPISLARLVMEK----------TPHIYLAFDAAEAFAR 61

Query: 200 TKGICLPETIAEANEWLVTERAKAQWKRYKSMVERAKKEKGTTNVVHSSSPQDNARISEL 259
             G+   ET+  +                                 H  +P++ AR+ + 
Sbjct: 62  AHGV---ETVDSS---------------------------------HFITPENIARLKQA 121

Query: 260 QSHNPPE-GTTCQSSTRSNEEEDCTMDTVGVICVDSEGHVASGASSGGIAMKVSGRVGLA 319
           +  N  +   T  S    +   D  + TVG + VDS G++AS  S+GG   K+ GR+G  
Sbjct: 122 KEFNRVQLDYTVPSPKVPDNCGDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDT 181

Query: 320 AMYGSGCWASSKGPFGAPFMVGGCVSGGGEYLMKGFAARECCVSLSLSQAGPASACMKVL 379
            + G+G +A+      A        +G GE +++G  AR+    +         A   V+
Sbjct: 182 PVIGAGTYANHLCAISA--------TGKGEDIIRGTVARDVAALMEYKGLSLTEAAAYVV 212

Query: 380 RNFVQESSQHNADKSAGILLVQADASKLAPGN 411
              V          S G++ V A+     P N
Sbjct: 242 DQSVPRG-------SCGLVAVSANGEVTMPFN 212

The following BLAST results are available for this feature:

BLAST of CSPI03G08990.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65268	5.3e-145	65.38	Putative threonine aspartase OS=Arabidopsis thaliana OX=3702 GN=At4g00590 PE=2 S...	[more]
Q9H6P5	1.6e-45	36.69	Threonine aspartase 1 OS=Homo sapiens OX=9606 GN=TASP1 PE=1 SV=1	[more]
Q8R1G1	2.8e-45	37.98	Threonine aspartase 1 OS=Mus musculus OX=10090 GN=Tasp1 PE=2 SV=1	[more]
Q9P6N7	1.3e-21	26.60	Putative threonine aspartase OS=Schizosaccharomyces pombe (strain 972 / ATCC 248...	[more]
Q6GM78	1.2e-19	32.53	Isoaspartyl peptidase/L-asparaginase OS=Xenopus laevis OX=8355 GN=asrgl1 PE=2 SV...	[more]

BLAST of CSPI03G08990.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L7I2	6.0e-261	99.58	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G122610 PE=4 SV=1	[more]
A0A5D3D030	8.8e-220	95.72	Putative threonine aspartase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A1S3AVH3	8.8e-220	95.72	putative threonine aspartase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103483292 ...	[more]
A0A1S4DSV0	6.3e-218	95.49	putative threonine aspartase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103483292 ...	[more]
A0A1S3AW50	3.8e-207	91.69	putative threonine aspartase isoform X3 OS=Cucumis melo OX=3656 GN=LOC103483292 ...	[more]

BLAST of CSPI03G08990.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650761.2	1.2e-260	99.58	putative threonine aspartase isoform X1 [Cucumis sativus]	[more]
KGN56576.2	6.7e-230	100.00	hypothetical protein Csa_011804 [Cucumis sativus]	[more]
XP_008438074.1	1.8e-219	95.72	PREDICTED: putative threonine aspartase isoform X1 [Cucumis melo] >KAA0048983.1 ...	[more]
XP_016899072.1	1.3e-217	95.49	PREDICTED: putative threonine aspartase isoform X2 [Cucumis melo]	[more]
XP_011650762.1	2.9e-217	95.93	putative threonine aspartase isoform X2 [Cucumis sativus]	[more]

BLAST of CSPI03G08990.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G00590.1	3.7e-146	65.38	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G00590.2	2.0e-131	61.06	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT3G16150.1	3.0e-18	28.67	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT5G08100.1	8.1e-16	26.44	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT5G08100.2	1.3e-05	24.26	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000246	Peptidase T2, asparaginase 2	PFAM	PF01112	Asparaginase_2	coord: 67..401 e-value: 8.2E-49 score: 166.3
IPR000246	Peptidase T2, asparaginase 2	PANTHER	PTHR10188	L-ASPARAGINASE	coord: 60..444
None	No IPR available	GENE3D	3.60.20.30	(Glycosyl)asparaginase	coord: 286..454 e-value: 2.6E-30 score: 107.4
None	No IPR available	PIRSR	PIRSR600246-1	PIRSR600246-1	coord: 64..438 e-value: 1.5E-68 score: 229.1
None	No IPR available	PIRSR	PIRSR600246-3	PIRSR600246-3	coord: 64..438 e-value: 1.5E-68 score: 229.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 236..278
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 242..277
None	No IPR available	PANTHER	PTHR10188:SF8	THREONINE ASPARTASE 1	coord: 60..444
IPR037464	Threonine aspartase 1	CDD	cd04514	Taspase1_like	coord: 65..466 e-value: 3.42975E-123 score: 359.663
IPR029055	Nucleophile aminohydrolases, N-terminal	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 64..428

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G08990	CSPI03G08990	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G08990.1.utr3p1	CSPI03G08990.1.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G08990.1.cds11	CSPI03G08990.1.cds11	CDS
CSPI03G08990.1.cds10	CSPI03G08990.1.cds10	CDS
CSPI03G08990.1.cds9	CSPI03G08990.1.cds9	CDS
CSPI03G08990.1.cds8	CSPI03G08990.1.cds8	CDS
CSPI03G08990.1.cds7	CSPI03G08990.1.cds7	CDS
CSPI03G08990.1.cds6	CSPI03G08990.1.cds6	CDS
CSPI03G08990.1.cds5	CSPI03G08990.1.cds5	CDS
CSPI03G08990.1.cds4	CSPI03G08990.1.cds4	CDS
CSPI03G08990.1.cds3	CSPI03G08990.1.cds3	CDS
CSPI03G08990.1.cds2	CSPI03G08990.1.cds2	CDS
CSPI03G08990.1.cds1	CSPI03G08990.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G08990.1	CSPI03G08990.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0004298	threonine-type endopeptidase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen