CSPI03G06580.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI03G06580.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Heat-shock protein
Location	Chr3: 5767479 .. 5768148 (-)
Sequence length	670
RNA-Seq Expression	CSPI03G06580.1
Synteny	CSPI03G06580.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCAATCTAATTCCAGCCATCCTCTGCCTCCTCACGGTGGCGTTCTTGGCAGCGCAGCTCACTGAGAGCTTCATGCCGTACACTGGAGCGCCGTGGGGCACGGTGGTGCCGTCCGACGACCCATTTAGAATCCTTGAACAAATGCCTCTAACAGTCCCAAGGGGAATGGAAACAATGGCATTGGCACAAGTGGATTGGAAAGAGACACCATTTGAGCACAAGATCTTGATAGACATTCCAGGGATGAAGAAGGAAGATGTGAAAGTGGAGGTGGAGGAGAACAGAGTATTAAGGATCAGCGGCGAACGGAAGGCGGAGACGGAGGTGGCGATGGCGACCGAGGAAGGAGAGAAGTGGCACAGAGCAGAGAGGGTAAATGGGAAATTTTGGAGGCAATTTAGAATGCCTGGAAATGTAAATTTGGATGGAATTAAGGCAAGTCTTGAAGATGGGGTACTCATAATTAGAGTGCCTAAATTAGTGGAAGAGAGGAGGAGGCAGCCTAAGATTATAAGTGTGGTGGGGGAAAGGCCTTCTGTTGGAGAAACTGATATCAAAGTCTCTAAAGATGAGATGTGATGATGTAAGAGAGATCATACCAAAATGGTTGAAATTATGTATGGTATGTTGCAAAAGGGTGCTTTATTGAATCTGATGTATAAAATGT

mRNA sequence

ATGGGCAATCTAATTCCAGCCATCCTCTGCCTCCTCACGGTGGCGTTCTTGGCAGCGCAGCTCACTGAGAGCTTCATGCCGTACACTGGAGCGCCGTGGGGCACGGTGGTGCCGTCCGACGACCCATTTAGAATCCTTGAACAAATGCCTCTAACAGTCCCAAGGGGAATGGAAACAATGGCATTGGCACAAGTGGATTGGAAAGAGACACCATTTGAGCACAAGATCTTGATAGACATTCCAGGGATGAAGAAGGAAGATGTGAAAGTGGAGGTGGAGGAGAACAGAGTATTAAGGATCAGCGGCGAACGGAAGGCGGAGACGGAGGTGGCGATGGCGACCGAGGAAGGAGAGAAGTGGCACAGAGCAGAGAGGGTAAATGGGAAATTTTGGAGGCAATTTAGAATGCCTGGAAATGTAAATTTGGATGGAATTAAGGCAAGTCTTGAAGATGGGGTACTCATAATTAGAGTGCCTAAATTAGTGGAAGAGAGGAGGAGGCAGCCTAAGATTATAAGTGTGGTGGGGGAAAGGCCTTCTGTTGGAGAAACTGATATCAAAGTCTCTAAAGATGAGATGTGATGATGTAAGAGAGATCATACCAAAATGGTTGAAATTATGTATGGTATGTTGCAAAAGGGTGCTTTATTGAATCTGATGTATAAAATGT

Coding sequence (CDS)

ATGGGCAATCTAATTCCAGCCATCCTCTGCCTCCTCACGGTGGCGTTCTTGGCAGCGCAGCTCACTGAGAGCTTCATGCCGTACACTGGAGCGCCGTGGGGCACGGTGGTGCCGTCCGACGACCCATTTAGAATCCTTGAACAAATGCCTCTAACAGTCCCAAGGGGAATGGAAACAATGGCATTGGCACAAGTGGATTGGAAAGAGACACCATTTGAGCACAAGATCTTGATAGACATTCCAGGGATGAAGAAGGAAGATGTGAAAGTGGAGGTGGAGGAGAACAGAGTATTAAGGATCAGCGGCGAACGGAAGGCGGAGACGGAGGTGGCGATGGCGACCGAGGAAGGAGAGAAGTGGCACAGAGCAGAGAGGGTAAATGGGAAATTTTGGAGGCAATTTAGAATGCCTGGAAATGTAAATTTGGATGGAATTAAGGCAAGTCTTGAAGATGGGGTACTCATAATTAGAGTGCCTAAATTAGTGGAAGAGAGGAGGAGGCAGCCTAAGATTATAAGTGTGGTGGGGGAAAGGCCTTCTGTTGGAGAAACTGATATCAAAGTCTCTAAAGATGAGATGTGA

Protein sequence

MGNLIPAILCLLTVAFLAAQLTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETMALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKWHRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPSVGETDIKVSKDEM*

Homology

[Close]

BLAST of CSPI03G06580.1 vs. ExPASy Swiss-Prot
Match: P30236 (22.0 kDa class IV heat shock protein OS=Glycine max OX=3847 GN=HSP22.0 PE=3 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 3.0e-38
Identity = 88/200 (44.00%), Postives = 125/200 (62.50%), Query Frame = 0


Query: 3   NLIPAILCLLTVAFLAAQLTESFMPYTGAP-------WGTVVPSDDPFRILEQMPLTVPR 62
           NL   +LC+       A+   S +P+   P       W    P  DPFR+LE +P  V +
Sbjct: 7   NLFFLLLCV-------AKANGSLLPFMDPPITLLADLWSDRFP--DPFRVLEHIPFGVDK 66

Query: 63  GMETMAL--AQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMA 122
              +MA+  A+VDWKETP  H I++D+PG+K+E++KVEVEENRVLR+SGERK E E    
Sbjct: 67  DEASMAMSPARVDWKETPEGHVIMLDVPGLKREEIKVEVEENRVLRVSGERKKEEE---- 126

Query: 123 TEEGEKWHRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIIS 182
            ++G+ WHR ER  GKFWRQFR+P NV+LD +KA LE+GVL + + KL   + + P+++S
Sbjct: 127 -KKGDHWHRVERSYGKFWRQFRLPQNVDLDSVKAKLENGVLTLTLDKLSPGKIKGPRVVS 186

Query: 183 VVGERPSVGETDIKVSKDEM 194
           + GE    G  +   +K E+
Sbjct: 187 IAGEDHQQGNLNNDGAKQEL 192

[Close]

BLAST of CSPI03G06580.1 vs. ExPASy Swiss-Prot
Match: P19244 (22.7 kDa class IV heat shock protein OS=Pisum sativum OX=3888 GN=HSP22.7 PE=2 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 3.3e-37
Identity = 87/201 (43.28%), Postives = 130/201 (64.68%), Query Frame = 0


Query: 4   LIPAILCLLTVAFLAAQLTESFMPYTGAP-------WGTVVPSDDPFRILEQMPLTVPRG 63
           L+P +L +L   F   +   S +P+  +P       W    P  DPFR+LEQ+P  V + 
Sbjct: 10  LVPFLLLILAADF-PLKAKASLLPFIDSPNTLLSDLWSDRFP--DPFRVLEQIPYGVEKH 69

Query: 64  METMAL--AQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMAT 123
             ++ L  A+VDWKETP  H I++D+PG+KK+D+K+EVEENRVLR+SGERK E +     
Sbjct: 70  EPSITLSHARVDWKETPEGHVIMVDVPGLKKDDIKIEVEENRVLRVSGERKKEED----- 129

Query: 124 EEGEKWHRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISV 183
           ++G+ WHR ER  GKFWRQF++P NV+LD +KA +E+GVL + + KL  ++ + P+++S+
Sbjct: 130 KKGDHWHRVERSYGKFWRQFKLPQNVDLDSVKAKMENGVLTLTLHKLSHDKIKGPRMVSI 189

Query: 184 VGE--RPSVGETDIKVSKDEM 194
           V E  +PS      K+  DE+
Sbjct: 190 VEEDDKPS------KIVNDEL 196

[Close]

BLAST of CSPI03G06580.1 vs. ExPASy Swiss-Prot
Match: Q38806 (22.0 kDa heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP22.0 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 4.1e-35
Identity = 72/152 (47.37%), Postives = 108/152 (71.05%), Query Frame = 0


Query: 41  DPFRILEQMPLTVPRGME-TMALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLR 100
           DPF+ILE++PL + R     ++ A+VDWKET   H+I++DIPG+KK++VK+EVEEN VLR
Sbjct: 48  DPFKILERIPLGLERDTSVALSPARVDWKETAEGHEIMLDIPGLKKDEVKIEVEENGVLR 107

Query: 101 ISGERKAETEVAMATEEGEKWHRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVP 160
           +SGERK E E     ++G++WHR ER  GKFWRQF++P NV+++ +KA LE+GVL I + 
Sbjct: 108 VSGERKREEE-----KKGDQWHRVERSYGKFWRQFKLPDNVDMESVKAKLENGVLTINLT 167

Query: 161 KLVEERRRQPKIISVVGERPSVGETDIKVSKD 192
           KL  E+ + P+++++  E     +     SK+
Sbjct: 168 KLSPEKVKGPRVVNIAAEEDQTAKISSSESKE 194

[Close]

BLAST of CSPI03G06580.1 vs. ExPASy Swiss-Prot
Match: Q53M11 (21.9 kDa heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP21.9 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 2.5e-32
Identity = 80/194 (41.24%), Postives = 116/194 (59.79%), Query Frame = 0


Query: 14  VAFLAAQLTESFMPYTG-APWGTVVPSDDPFRILEQMPLTVPRGM---------ETMALA 73
           VA +AA +     P     P+G     DDPFR+LEQ PL    G+           +ALA
Sbjct: 13  VAAVAAMVVMMAPPAAALVPYGYGYMLDDPFRVLEQSPLRPAGGVAAAAAAGEPAAVALA 72

Query: 74  QVDWKETPFEHKILIDIPGMKKEDVKVEVEE-NRVLRISGERK--AETEVAMATEEGEKW 133
           + DWKETP  H + +D+PG+++ DV+VEV+E +RVLR+SGER+     E      +G +W
Sbjct: 73  RCDWKETPEAHVVTVDVPGVRRGDVRVEVDEASRVLRVSGERRRAGAAEEEEGERDGVRW 132

Query: 134 HRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPS 193
           HRAER  G+FWR+FRMP   ++  + A L+DGVL + VPK+   R R+P+++++ G    
Sbjct: 133 HRAERAAGRFWRRFRMPPGADVGRVAARLDDGVLTVTVPKVPGHRGREPRVVAIDGAGAG 192

[Close]

BLAST of CSPI03G06580.1 vs. ExPASy Swiss-Prot
Match: Q7XUW5 (23.2 kDa heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP23.2 PE=2 SV=2)

HSP 1 Score: 139.0 bits (349), Expect = 5.5e-32
Identity = 75/191 (39.27%), Postives = 117/191 (61.26%), Query Frame = 0


Query: 7   AILCLLTVAFLAAQLTESFMPYTG--------APWGTVVPSDDPFRILEQMPLTVPR-GM 66
           A+L  + V   +     + +P+ G         P   ++ + DPFRILE +P    R  +
Sbjct: 11  AMLACIAVVLASTAADGALLPWFGGGGARDEAVPELGLLAAADPFRILEHVPFGFDRDDV 70

Query: 67  ETMALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEG 126
             +++A+VDW+ET   H++++D+PGM+KED++VEVE+NRVLRISGER+ E E       G
Sbjct: 71  AMLSMARVDWRETGDAHEVVVDVPGMRKEDLRVEVEDNRVLRISGERRRE-ETTEQKGGG 130

Query: 127 EKWHRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVV-- 183
           + WHR ER  G+FWRQ R+P N +LD I ASL++GVL +R  KL  ++ + P+++ +   
Sbjct: 131 DHWHREERSYGRFWRQLRLPDNADLDSIAASLDNGVLTVRFRKLAPDQIKGPRVVGIASA 190

[Close]

BLAST of CSPI03G06580.1 vs. ExPASy TrEMBL
Match: A0A0A0L6S3 (Heat-shock protein OS=Cucumis sativus OX=3659 GN=Csa_3G113300 PE=3 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 3.1e-102
Identity = 192/193 (99.48%), Postives = 192/193 (99.48%), Query Frame = 0


Query: 1   MGNLIPAILCLLTVAFLAAQLTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETM 60
           MGNLIPAILCLLTVAFLAAQ TESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETM
Sbjct: 1   MGNLIPAILCLLTVAFLAAQRTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETM 60

Query: 61  ALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKW 120
           ALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKW
Sbjct: 61  ALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKW 120

Query: 121 HRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPS 180
           HRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPS
Sbjct: 121 HRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPS 180

Query: 181 VGETDIKVSKDEM 194
           VGETDIKVSKDEM
Sbjct: 181 VGETDIKVSKDEM 193

[Close]

BLAST of CSPI03G06580.1 vs. ExPASy TrEMBL
Match: A0A5D3BNX9 (22.0 kDa class IV heat shock protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold420G00060 PE=3 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 5.1e-97
Identity = 181/193 (93.78%), Postives = 187/193 (96.89%), Query Frame = 0


Query: 1   MGNLIPAILCLLTVAFLAAQLTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETM 60
           MGN IPAILCLLTVAF+AAQ TESFMPYTGAPWGTVVPSDDPFRILEQMPLTVP GMETM
Sbjct: 1   MGNQIPAILCLLTVAFMAAQHTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPTGMETM 60

Query: 61  ALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKW 120
           ALAQVDWKETPFEHKILIDIPGMKKEDVKVE+EENRVLRISGERKAETE AM TEEGEKW
Sbjct: 61  ALAQVDWKETPFEHKILIDIPGMKKEDVKVELEENRVLRISGERKAETEAAMVTEEGEKW 120

Query: 121 HRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPS 180
           HRAERVNGKFWRQFR+PGN +LDGIKASLEDGVLIIRVPKLVEERR+QPKIISVVGERPS
Sbjct: 121 HRAERVNGKFWRQFRIPGNADLDGIKASLEDGVLIIRVPKLVEERRKQPKIISVVGERPS 180

Query: 181 VGETDIKVSKDEM 194
           VGETDIKV+KDEM
Sbjct: 181 VGETDIKVAKDEM 193

[Close]

BLAST of CSPI03G06580.1 vs. ExPASy TrEMBL
Match: A0A1S3BTB8 (22.0 kDa class IV heat shock protein-like OS=Cucumis melo OX=3656 GN=LOC103493293 PE=3 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 5.1e-97
Identity = 181/193 (93.78%), Postives = 187/193 (96.89%), Query Frame = 0


Query: 1   MGNLIPAILCLLTVAFLAAQLTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETM 60
           MGN IPAILCLLTVAF+AAQ TESFMPYTGAPWGTVVPSDDPFRILEQMPLTVP GMETM
Sbjct: 1   MGNQIPAILCLLTVAFMAAQHTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPTGMETM 60

Query: 61  ALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKW 120
           ALAQVDWKETPFEHKILIDIPGMKKEDVKVE+EENRVLRISGERKAETE AM TEEGEKW
Sbjct: 61  ALAQVDWKETPFEHKILIDIPGMKKEDVKVELEENRVLRISGERKAETEAAMVTEEGEKW 120

Query: 121 HRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPS 180
           HRAERVNGKFWRQFR+PGN +LDGIKASLEDGVLIIRVPKLVEERR+QPKIISVVGERPS
Sbjct: 121 HRAERVNGKFWRQFRIPGNADLDGIKASLEDGVLIIRVPKLVEERRKQPKIISVVGERPS 180

Query: 181 VGETDIKVSKDEM 194
           VGETDIKV+KDEM
Sbjct: 181 VGETDIKVAKDEM 193

[Close]

BLAST of CSPI03G06580.1 vs. ExPASy TrEMBL
Match: A0A7J7CGG6 (HSP20-like chaperones superfamily protein OS=Tripterygium wilfordii OX=458696 GN=HS088_TW17G00681 PE=3 SV=1)

HSP 1 Score: 226.1 bits (575), Expect = 1.3e-55
Identity = 116/191 (60.73%), Postives = 145/191 (75.92%), Query Frame = 0


Query: 4   LIPAILCLLTVAFLAAQLTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMET-MAL 63
           LIP +L LL +  +A+Q   + +PYT + W  ++P++DPFRILEQ PLT+P+ MET +AL
Sbjct: 13  LIPQMLALLVLCLMASQ-GNALVPYTRSLWDMMLPTEDPFRILEQTPLTIPKSMETPLAL 72

Query: 64  AQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKWHR 123
           A+ DWKETP  H I +DIPGMKK+DVK+EVEENRVLRISGERK E E+     EGEKWHR
Sbjct: 73  ARADWKETPTSHVITLDIPGMKKDDVKIEVEENRVLRISGERKDEEEI-----EGEKWHR 132

Query: 124 AERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPSVG 183
            ER NGKFWRQFR+P N +LD IKA LEDGVL I VPK  E+++RQPK+I++ GE  S  
Sbjct: 133 VERTNGKFWRQFRLPANADLDQIKAHLEDGVLRITVPKFAEQKKRQPKVINIAGEEGSSA 192

Query: 184 ETDIKVSKDEM 194
           + DIK +K EM
Sbjct: 193 Q-DIKATKAEM 196

[Close]

BLAST of CSPI03G06580.1 vs. ExPASy TrEMBL
Match: A0A6N2AQT0 (SHSP domain-containing protein OS=Solanum chilense OX=4083 GN=EJD97_025781 PE=3 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 1.8e-54
Identity = 111/187 (59.36%), Postives = 138/187 (73.80%), Query Frame = 0


Query: 7   AILCLLTVAFLAAQLTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETMALAQVD 66
           + L L  V  L     ++ MPYT   W    P +DPFRILEQ PLT+P+G+E++AL + D
Sbjct: 10  SFLVLAMVVVLFPSQIKALMPYTRPFWDIAFPPEDPFRILEQTPLTIPKGVESIALTRSD 69

Query: 67  WKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKWHRAERV 126
           WKET  EH I +DIPGMKKED+K+EVEENRVLR+SGERK E E+     EGEKWHRAER 
Sbjct: 70  WKETATEHVITLDIPGMKKEDIKIEVEENRVLRVSGERKTEEEI-----EGEKWHRAERT 129

Query: 127 NGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPSVGETDI 186
            GKFWRQFR+PGN +L+ IKA+LE+GVL I VPKL EE+++Q K+IS+  ER +VG  DI
Sbjct: 130 CGKFWRQFRLPGNADLEHIKANLENGVLKITVPKLAEEKKKQSKVISIAEERKAVGGEDI 189

Query: 187 KVSKDEM 194
           K +K EM
Sbjct: 190 KANKAEM 191

[Close]

BLAST of CSPI03G06580.1 vs. NCBI nr
Match: XP_004133737.1 (22.0 kDa class IV heat shock protein [Cucumis sativus])

HSP 1 Score: 380.9 bits (977), Expect = 6.3e-102
Identity = 192/193 (99.48%), Postives = 192/193 (99.48%), Query Frame = 0


Query: 1   MGNLIPAILCLLTVAFLAAQLTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETM 60
           MGNLIPAILCLLTVAFLAAQ TESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETM
Sbjct: 1   MGNLIPAILCLLTVAFLAAQRTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETM 60

Query: 61  ALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKW 120
           ALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKW
Sbjct: 61  ALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKW 120

Query: 121 HRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPS 180
           HRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPS
Sbjct: 121 HRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPS 180

Query: 181 VGETDIKVSKDEM 194
           VGETDIKVSKDEM
Sbjct: 181 VGETDIKVSKDEM 193

[Close]

BLAST of CSPI03G06580.1 vs. NCBI nr
Match: KAE8650195.1 (hypothetical protein Csa_011521 [Cucumis sativus])

HSP 1 Score: 370.9 bits (951), Expect = 6.6e-99
Identity = 186/188 (98.94%), Postives = 187/188 (99.47%), Query Frame = 0


Query: 1   MGNLIPAILCLLTVAFLAAQLTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETM 60
           MGNLIPAILCLLTVAFLAAQ TESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETM
Sbjct: 1   MGNLIPAILCLLTVAFLAAQRTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETM 60

Query: 61  ALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKW 120
           ALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKW
Sbjct: 61  ALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKW 120

Query: 121 HRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPS 180
           HRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPS
Sbjct: 121 HRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPS 180

Query: 181 VGETDIKV 189
           VGETDIK+
Sbjct: 181 VGETDIKI 188

[Close]

BLAST of CSPI03G06580.1 vs. NCBI nr
Match: XP_008452205.1 (PREDICTED: 22.0 kDa class IV heat shock protein-like [Cucumis melo] >KAA0060510.1 22.0 kDa class IV heat shock protein-like [Cucumis melo var. makuwa] >TYK00750.1 22.0 kDa class IV heat shock protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 363.6 bits (932), Expect = 1.0e-96
Identity = 181/193 (93.78%), Postives = 187/193 (96.89%), Query Frame = 0


Query: 1   MGNLIPAILCLLTVAFLAAQLTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETM 60
           MGN IPAILCLLTVAF+AAQ TESFMPYTGAPWGTVVPSDDPFRILEQMPLTVP GMETM
Sbjct: 1   MGNQIPAILCLLTVAFMAAQHTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPTGMETM 60

Query: 61  ALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKW 120
           ALAQVDWKETPFEHKILIDIPGMKKEDVKVE+EENRVLRISGERKAETE AM TEEGEKW
Sbjct: 61  ALAQVDWKETPFEHKILIDIPGMKKEDVKVELEENRVLRISGERKAETEAAMVTEEGEKW 120

Query: 121 HRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPS 180
           HRAERVNGKFWRQFR+PGN +LDGIKASLEDGVLIIRVPKLVEERR+QPKIISVVGERPS
Sbjct: 121 HRAERVNGKFWRQFRIPGNADLDGIKASLEDGVLIIRVPKLVEERRKQPKIISVVGERPS 180

Query: 181 VGETDIKVSKDEM 194
           VGETDIKV+KDEM
Sbjct: 181 VGETDIKVAKDEM 193

[Close]

BLAST of CSPI03G06580.1 vs. NCBI nr
Match: XP_038905611.1 (22.7 kDa class IV heat shock protein-like [Benincasa hispida])

HSP 1 Score: 324.7 bits (831), Expect = 5.4e-85
Identity = 163/193 (84.46%), Postives = 174/193 (90.16%), Query Frame = 0


Query: 1   MGNLIPAILCLLTVAFLAAQLTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETM 60
           M N IP +LCLLT AF A +  ESF+PYTGAPWGTVVPSDDPFRILEQM LT+PRGMET+
Sbjct: 1   MSNPIPLLLCLLTAAFTAVREAESFVPYTGAPWGTVVPSDDPFRILEQMTLTIPRGMETV 60

Query: 61  ALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKW 120
           ALAQVDWKET FEHKILIDIPGMKKEDVKVEVEENRVLRISGER+A    A A EEGE+W
Sbjct: 61  ALAQVDWKETSFEHKILIDIPGMKKEDVKVEVEENRVLRISGERRA----AAAVEEGERW 120

Query: 121 HRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPS 180
           HRAERVNGKFWRQFRMPGN NLDGIKA+LEDGVL IRVPKLVEE+RRQPKIISV+GERPS
Sbjct: 121 HRAERVNGKFWRQFRMPGNANLDGIKANLEDGVLKIRVPKLVEEKRRQPKIISVLGERPS 180

Query: 181 VGETDIKVSKDEM 194
            GETD+KVSKDEM
Sbjct: 181 DGETDLKVSKDEM 189

[Close]

BLAST of CSPI03G06580.1 vs. NCBI nr
Match: XP_027358791.1 (22.7 kDa class IV heat shock protein-like [Abrus precatorius])

HSP 1 Score: 227.3 bits (578), Expect = 1.2e-55
Identity = 113/186 (60.75%), Postives = 145/186 (77.96%), Query Frame = 0


Query: 7   AILCLLTVAFLAAQLTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETMALAQVD 66
           +++ ++T+ FL+   T + MPYT   W  ++PSDDPFRILEQ PL +P+G+ET+ALA+ D
Sbjct: 10  SLVTVVTLLFLSIGTTNALMPYTRTLWDMMLPSDDPFRILEQTPLNIPKGIETLALARAD 69

Query: 67  WKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKWHRAERV 126
           WKETP EH I++D+PG+KK DVK+EVEENRVLRISGERK E E     +E EKWHRAER 
Sbjct: 70  WKETPSEHVIVLDLPGLKKNDVKIEVEENRVLRISGERKGEEE-----KENEKWHRAERT 129

Query: 127 NGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVGERPSVGETDI 186
           NGKFWRQFR+P N +L+ +KASLEDGVL I VPKL E+++RQPKII++  E  SV E D+
Sbjct: 130 NGKFWRQFRLPSNADLEHVKASLEDGVLKITVPKLAEDKKRQPKIINIAQEE-SVAE-DV 188

Query: 187 KVSKDE 193
           K +K E
Sbjct: 190 KATKAE 188

[Close]

BLAST of CSPI03G06580.1 vs. TAIR 10
Match: AT4G10250.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 2.9e-36
Identity = 72/152 (47.37%), Postives = 108/152 (71.05%), Query Frame = 0


Query: 41  DPFRILEQMPLTVPRGME-TMALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLR 100
           DPF+ILE++PL + R     ++ A+VDWKET   H+I++DIPG+KK++VK+EVEEN VLR
Sbjct: 48  DPFKILERIPLGLERDTSVALSPARVDWKETAEGHEIMLDIPGLKKDEVKIEVEENGVLR 107

Query: 101 ISGERKAETEVAMATEEGEKWHRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVP 160
           +SGERK E E     ++G++WHR ER  GKFWRQF++P NV+++ +KA LE+GVL I + 
Sbjct: 108 VSGERKREEE-----KKGDQWHRVERSYGKFWRQFKLPDNVDMESVKAKLENGVLTINLT 167

Query: 161 KLVEERRRQPKIISVVGERPSVGETDIKVSKD 192
           KL  E+ + P+++++  E     +     SK+
Sbjct: 168 KLSPEKVKGPRVVNIAAEEDQTAKISSSESKE 194

[Close]

BLAST of CSPI03G06580.1 vs. TAIR 10
Match: AT1G07400.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 118.6 bits (296), Expect = 5.5e-27
Identity = 64/137 (46.72%), Postives = 92/137 (67.15%), Query Frame = 0


Query: 41  DPFRILEQMPLTVPRGMETMALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRI 100
           DPF+ L Q P ++      +  A+VDWKET   H    D+PGMKKE+VKVE+E++ VL+I
Sbjct: 27  DPFKEL-QFPSSLSGETSAITNARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDSVLKI 86

Query: 101 SGERKAETEVAMATEEGEKWHRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPK 160
           SGER  E E     E+ + WHR ER +G+F R+F++P NV +D +KAS+E+GVL + VPK
Sbjct: 87  SGERHVEKE-----EKQDTWHRVERSSGQFSRKFKLPENVKMDQVKASMENGVLTVTVPK 146

Query: 161 LVEERRR-QPKIISVVG 177
           + E +++ Q K I + G
Sbjct: 147 VEEAKKKAQVKSIDISG 157

[Close]

BLAST of CSPI03G06580.1 vs. TAIR 10
Match: AT3G46230.1 (heat shock protein 17.4 )

HSP 1 Score: 118.2 bits (295), Expect = 7.1e-27
Identity = 68/167 (40.72%), Postives = 98/167 (58.68%), Query Frame = 0


Query: 12  LTVAFLAAQLTESFMPYTGAPWGTVVPSDDPFRILEQMPLTVPRGMETMAL--AQVDWKE 71
           L  +F   + T  F P++   W       DPF       LT     +  A   A+VDW+E
Sbjct: 3   LVPSFFGGRRTNVFDPFSLDVW-------DPFEGFLTPGLTNAPAKDVAAFTNAKVDWRE 62

Query: 72  TPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMATEEGEKWHRAERVNGK 131
           TP  H    D+PG+KKE+VKVEVE+  +L+ISGER +E E     E+ + WHR ER +GK
Sbjct: 63  TPEAHVFKADVPGLKKEEVKVEVEDGNILQISGERSSENE-----EKSDTWHRVERSSGK 122

Query: 132 FWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISVVG 177
           F R+FR+P N  ++ +KAS+E+GVL + VPK V+E + + K + + G
Sbjct: 123 FMRRFRLPENAKVEEVKASMENGVLSVTVPK-VQESKPEVKSVDISG 156

[Close]

BLAST of CSPI03G06580.1 vs. TAIR 10
Match: AT5G59720.1 (heat shock protein 18.2 )

HSP 1 Score: 117.1 bits (292), Expect = 1.6e-26
Identity = 60/122 (49.18%), Postives = 82/122 (67.21%), Query Frame = 0


Query: 55  RGMETMALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRISGERKAETEVAMAT 114
           R +     A+VDWKETP  H    D+PG+KKE+VKVEVE+  VL+ISGER  E E     
Sbjct: 44  RDVAAFTNARVDWKETPEAHVFKADLPGLKKEEVKVEVEDKNVLQISGERSKENE----- 103

Query: 115 EEGEKWHRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPKLVEERRRQPKIISV 174
           E+ +KWHR ER +GKF R+FR+P N  ++ +KA++E+GVL + VPK   E++ Q K I +
Sbjct: 104 EKNDKWHRVERASGKFMRRFRLPENAKMEEVKATMENGVLTVVVPK-APEKKPQVKSIDI 159

Query: 175 VG 177
            G
Sbjct: 164 SG 159

[Close]

BLAST of CSPI03G06580.1 vs. TAIR 10
Match: AT2G29500.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 2.1e-26
Identity = 62/136 (45.59%), Postives = 89/136 (65.44%), Query Frame = 0


Query: 41  DPFRILEQMPLTVPRGMETMALAQVDWKETPFEHKILIDIPGMKKEDVKVEVEENRVLRI 100
           DPF+ L    L+  R    +  A+VDW+ETP  H    D+PG+KKE+VKVE+EE+ VL+I
Sbjct: 26  DPFKELTSSSLS--RENSAIVNARVDWRETPEAHVFKADLPGLKKEEVKVEIEEDSVLKI 85

Query: 101 SGERKAETEVAMATEEGEKWHRAERVNGKFWRQFRMPGNVNLDGIKASLEDGVLIIRVPK 160
           SGER  E E     ++ + WHR ER +G+F R+FR+P NV +D +KA++E+GVL + VPK
Sbjct: 86  SGERHVEKE-----DKNDTWHRVERSSGQFTRRFRLPENVKMDQVKAAMENGVLTVTVPK 145

Query: 161 LVEERRRQPKIISVVG 177
             E ++   K I + G
Sbjct: 146 -AETKKADVKSIQISG 153

The following BLAST results are available for this feature:

BLAST of CSPI03G06580.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P30236	3.0e-38	44.00	22.0 kDa class IV heat shock protein OS=Glycine max OX=3847 GN=HSP22.0 PE=3 SV=1	[more]
P19244	3.3e-37	43.28	22.7 kDa class IV heat shock protein OS=Pisum sativum OX=3888 GN=HSP22.7 PE=2 SV...	[more]
Q38806	4.1e-35	47.37	22.0 kDa heat shock protein OS=Arabidopsis thaliana OX=3702 GN=HSP22.0 PE=2 SV=1	[more]
Q53M11	2.5e-32	41.24	21.9 kDa heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP21.9 ...	[more]
Q7XUW5	5.5e-32	39.27	23.2 kDa heat shock protein OS=Oryza sativa subsp. japonica OX=39947 GN=HSP23.2 ...	[more]

BLAST of CSPI03G06580.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L6S3	3.1e-102	99.48	Heat-shock protein OS=Cucumis sativus OX=3659 GN=Csa_3G113300 PE=3 SV=1	[more]
A0A5D3BNX9	5.1e-97	93.78	22.0 kDa class IV heat shock protein-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BTB8	5.1e-97	93.78	22.0 kDa class IV heat shock protein-like OS=Cucumis melo OX=3656 GN=LOC10349329...	[more]
A0A7J7CGG6	1.3e-55	60.73	HSP20-like chaperones superfamily protein OS=Tripterygium wilfordii OX=458696 GN...	[more]
A0A6N2AQT0	1.8e-54	59.36	SHSP domain-containing protein OS=Solanum chilense OX=4083 GN=EJD97_025781 PE=3 ...	[more]

BLAST of CSPI03G06580.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004133737.1	6.3e-102	99.48	22.0 kDa class IV heat shock protein [Cucumis sativus]	[more]
KAE8650195.1	6.6e-99	98.94	hypothetical protein Csa_011521 [Cucumis sativus]	[more]
XP_008452205.1	1.0e-96	93.78	PREDICTED: 22.0 kDa class IV heat shock protein-like [Cucumis melo] >KAA0060510....	[more]
XP_038905611.1	5.4e-85	84.46	22.7 kDa class IV heat shock protein-like [Benincasa hispida]	[more]
XP_027358791.1	1.2e-55	60.75	22.7 kDa class IV heat shock protein-like [Abrus precatorius]	[more]

BLAST of CSPI03G06580.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10250.1	2.9e-36	47.37	HSP20-like chaperones superfamily protein	[more]
AT1G07400.1	5.5e-27	46.72	HSP20-like chaperones superfamily protein	[more]
AT3G46230.1	7.1e-27	40.72	heat shock protein 17.4	[more]
AT5G59720.1	1.6e-26	49.18	heat shock protein 18.2	[more]
AT2G29500.1	2.1e-26	45.59	HSP20-like chaperones superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 66..174 e-value: 8.6E-26 score: 90.1
IPR002068	Alpha crystallin/Hsp20 domain	PROSITE	PS01031	SHSP	coord: 56..175 score: 27.736937
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 37..176 e-value: 2.1E-36 score: 126.9
IPR008978	HSP20-like chaperone	SUPERFAMILY	49764	HSP20-like chaperones	coord: 40..171
None	No IPR available	PANTHER	PTHR11527:SF135	21.9 KDA HEAT SHOCK PROTEIN	coord: 14..180
None	No IPR available	CDD	cd06472	ACD_ScHsp26_like	coord: 64..160 e-value: 9.1065E-45 score: 141.674
IPR031107	Small heat shock protein HSP20	PANTHER	PTHR11527	HEAT-SHOCK PROTEIN 20 FAMILY MEMBER	coord: 14..180
IPR007052	CS domain	PROSITE	PS51203	CS	coord: 60..172 score: 8.504172

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G06580	CSPI03G06580	gene

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G06580.1.utr3p1	CSPI03G06580.1.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G06580.1.cds1	CSPI03G06580.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G06580.1	CSPI03G06580.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051259	protein complex oligomerization
biological_process	GO:0006457	protein folding
biological_process	GO:0009408	response to heat
biological_process	GO:0042542	response to hydrogen peroxide
biological_process	GO:0009651	response to salt stress
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0051082	unfolded protein binding