CSPI03G01400.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI03G01400.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Calcium-binding EF-hand family protein
Location	Chr3: 1011664 .. 1012155 (+)
Sequence length	492
RNA-Seq Expression	CSPI03G01400.1
Synteny	CSPI03G01400.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCAACAAACAACCGATTAAGTTAGACGACGAACAAATTGCGGAGTTGAGAGAAATCTTCCGGTCGTTTGATCGGAACAACGACGGGAGTTTGACTCAACTTGAACTCGGATCGCTTTTACGATCACTTGGTTTGAAGCCGAGTCCGGATCAGCTTGAGAGTTTGATAATGAAGGCAGATACGAACAGCAATGGATTAGTTGAATTTTCAGAGTTCGTAGCTTTGGTTGAGCCGGAGCTGGTATCGGCTAAATGCCCTTACACGGAGGAGCAATTAAAGCAGTTGTTTAGAATGTTCGATAGAGATGGGAATGGGTTCATTACTGCGGCCGAGTTGGCTCACTCGATGGCCAAACTCGGCCATGCTCTCACCGCGGAGGAACTCACCGGAATGATCAGGGAAGCCGATACCGATGGCGATGGCAGAATCAATTTTCAAGAGTTCTCTCAGGCAATTACTTCTGCTGCATTTGATAATTCCTGGGCATGA

mRNA sequence

ATGAGCAACAAACAACCGATTAAGTTAGACGACGAACAAATTGCGGAGTTGAGAGAAATCTTCCGGTCGTTTGATCGGAACAACGACGGGAGTTTGACTCAACTTGAACTCGGATCGCTTTTACGATCACTTGGTTTGAAGCCGAGTCCGGATCAGCTTGAGAGTTTGATAATGAAGGCAGATACGAACAGCAATGGATTAGTTGAATTTTCAGAGTTCGTAGCTTTGGTTGAGCCGGAGCTGGTATCGGCTAAATGCCCTTACACGGAGGAGCAATTAAAGCAGTTGTTTAGAATGTTCGATAGAGATGGGAATGGGTTCATTACTGCGGCCGAGTTGGCTCACTCGATGGCCAAACTCGGCCATGCTCTCACCGCGGAGGAACTCACCGGAATGATCAGGGAAGCCGATACCGATGGCGATGGCAGAATCAATTTTCAAGAGTTCTCTCAGGCAATTACTTCTGCTGCATTTGATAATTCCTGGGCATGA

Coding sequence (CDS)

ATGAGCAACAAACAACCGATTAAGTTAGACGACGAACAAATTGCGGAGTTGAGAGAAATCTTCCGGTCGTTTGATCGGAACAACGACGGGAGTTTGACTCAACTTGAACTCGGATCGCTTTTACGATCACTTGGTTTGAAGCCGAGTCCGGATCAGCTTGAGAGTTTGATAATGAAGGCAGATACGAACAGCAATGGATTAGTTGAATTTTCAGAGTTCGTAGCTTTGGTTGAGCCGGAGCTGGTATCGGCTAAATGCCCTTACACGGAGGAGCAATTAAAGCAGTTGTTTAGAATGTTCGATAGAGATGGGAATGGGTTCATTACTGCGGCCGAGTTGGCTCACTCGATGGCCAAACTCGGCCATGCTCTCACCGCGGAGGAACTCACCGGAATGATCAGGGAAGCCGATACCGATGGCGATGGCAGAATCAATTTTCAAGAGTTCTCTCAGGCAATTACTTCTGCTGCATTTGATAATTCCTGGGCATGA

Protein sequence

MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKADTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKLGHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA*

Homology

[Close]

BLAST of CSPI03G01400.1 vs. ExPASy Swiss-Prot
Match: Q9M8U1 (Probable calcium-binding protein CML18 OS=Arabidopsis thaliana OX=3702 GN=CML18 PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 8.1e-69
Identity = 131/157 (83.44%), Postives = 145/157 (92.36%), Query Frame = 0


Query: 6   PIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKADTNSN 65
           P KL DEQ+AELREIFRSFD+N DGSLT+LELGSLLRSLGLKPS DQL++LI KAD N+N
Sbjct: 10  PAKLGDEQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNN 69

Query: 66  GLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKLGHALT 125
           GLVEFSEFVALVEP+LV  KCPYT++QLK +FRMFDRDGNG+ITAAELAHSMAKLGHALT
Sbjct: 70  GLVEFSEFVALVEPDLV--KCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALT 129

Query: 126 AEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSW 163
           AEELTGMI+EAD DGDG I+FQEF QAITSAAFDN+W
Sbjct: 130 AEELTGMIKEADRDGDGCIDFQEFVQAITSAAFDNAW 164

[Close]

BLAST of CSPI03G01400.1 vs. ExPASy Swiss-Prot
Match: Q9LQN4 (Probable calcium-binding protein CML17 OS=Arabidopsis thaliana OX=3702 GN=CML17 PE=2 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 1.1e-65
Identity = 130/165 (78.79%), Postives = 143/165 (86.67%), Query Frame = 0


Query: 1   MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60
           MS+K   KLD+EQI ELREIFRSFDRN DGSLTQLELGSLLR+LG+KPSPDQ E+LI KA
Sbjct: 1   MSHKVSKKLDEEQINELREIFRSFDRNKDGSLTQLELGSLLRALGVKPSPDQFETLIDKA 60

Query: 61  DTNSNGLVEFSEFVALVEPELVS---AKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSM 120
           DT SNGLVEF EFVALV PEL+S      PYTEEQL +LFR+FD DGNGFITAAELAHSM
Sbjct: 61  DTKSNGLVEFPEFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSM 120

Query: 121 AKLGHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSW 163
           AKLGHALT  ELTGMI+EAD+DGDGRINFQEF++AI SAAFD+ W
Sbjct: 121 AKLGHALTVAELTGMIKEADSDGDGRINFQEFAKAINSAAFDDIW 165

[Close]

BLAST of CSPI03G01400.1 vs. ExPASy Swiss-Prot
Match: Q9AWK2 (Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 GN=CML11 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 1.9e-65
Identity = 123/157 (78.34%), Postives = 143/157 (91.08%), Query Frame = 0


Query: 4   KQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKADTN 63
           +Q   L D+Q+ ELREIFRSFDRN DGSLTQLELGSLLRSLGLKPS D+L+SLI +ADTN
Sbjct: 52  QQEEPLGDDQLGELREIFRSFDRNGDGSLTQLELGSLLRSLGLKPSTDELDSLIQRADTN 111

Query: 64  SNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKLGHA 123
           SNGL+EFSEFVALV PEL+  + PY+E+Q+++LF +FDRDGNGFITAAELAHSMAKLGHA
Sbjct: 112 SNGLIEFSEFVALVAPELLYDRAPYSEDQIRRLFNIFDRDGNGFITAAELAHSMAKLGHA 171

Query: 124 LTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDN 161
           LT +ELTGMI+EADTDGDGRI+FQEFS+AIT+AAFDN
Sbjct: 172 LTVKELTGMIKEADTDGDGRISFQEFSRAITAAAFDN 208

[Close]

BLAST of CSPI03G01400.1 vs. ExPASy Swiss-Prot
Match: Q6L5F4 (Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 GN=CML14 PE=2 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 7.2e-41
Identity = 85/154 (55.19%), Postives = 110/154 (71.43%), Query Frame = 0


Query: 6   PIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKADTNSN 65
           P +L   Q+ +LRE+FR FD N DGSLTQLEL +LLRSLGL+P+ D++ +L+   D N N
Sbjct: 15  PQQLRGSQLKQLRELFRRFDMNGDGSLTQLELAALLRSLGLRPTGDEVHALLAGMDANGN 74

Query: 66  GLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKLGHALT 125
           G VEF E  A + P L +      + QL ++FR FDRDGNGFI+AAELA SMA+LG  LT
Sbjct: 75  GSVEFDELAAAIAPVLTTQTHLVDQAQLLEVFRAFDRDGNGFISAAELARSMARLGQPLT 134

Query: 126 AEELTGMIREADTDGDGRINFQEFSQAITSAAFD 160
            EELT M+R+ADTDGDG I+F+EF+  +  +A D
Sbjct: 135 FEELTRMMRDADTDGDGVISFKEFAAVMAKSALD 168

[Close]

BLAST of CSPI03G01400.1 vs. ExPASy Swiss-Prot
Match: Q9LI84 (Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 PE=2 SV=2)

HSP 1 Score: 157.1 bits (396), Expect = 1.7e-37
Identity = 81/148 (54.73%), Postives = 106/148 (71.62%), Query Frame = 0


Query: 12  EQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKADTNSNGLVEFS 71
           +QI +L++IF  FD + DGSLTQLEL +LLRSLG+KP  DQ+  L+ + D N NG VEF 
Sbjct: 8   DQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFD 67

Query: 72  EFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKLGHALTAEELTG 131
           E V  + P+ ++ +    +EQL ++FR FDRDGNG ITAAELA SMAK+GH LT  ELT 
Sbjct: 68  ELVVAILPD-INEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTE 127

Query: 132 MIREADTDGDGRINFQEFSQAITSAAFD 160
           M+ EAD++GDG I+F EFS  +  +A D
Sbjct: 128 MMTEADSNGDGVISFNEFSHIMAKSAAD 154

[Close]

BLAST of CSPI03G01400.1 vs. ExPASy TrEMBL
Match: A0A0A0L1S8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G008850 PE=4 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 2.4e-84
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60
           MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA
Sbjct: 1   MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60

Query: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120
           DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL
Sbjct: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120

Query: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 164
           GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA
Sbjct: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 163

[Close]

BLAST of CSPI03G01400.1 vs. ExPASy TrEMBL
Match: A0A5A7UCP8 (Putative calcium-binding protein CML18 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G001390 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.1e-83
Identity = 161/163 (98.77%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60
           MS+KQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLE+LIMKA
Sbjct: 1   MSDKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLENLIMKA 60

Query: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120
           DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL
Sbjct: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120

Query: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 164
           GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA
Sbjct: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 163

[Close]

BLAST of CSPI03G01400.1 vs. ExPASy TrEMBL
Match: A0A1S3BKU3 (probable calcium-binding protein CML18 OS=Cucumis melo OX=3656 GN=LOC103490757 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.1e-83
Identity = 161/163 (98.77%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60
           MS+KQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLE+LIMKA
Sbjct: 1   MSDKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLENLIMKA 60

Query: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120
           DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL
Sbjct: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120

Query: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 164
           GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA
Sbjct: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 163

[Close]

BLAST of CSPI03G01400.1 vs. ExPASy TrEMBL
Match: A0A6J1EA11 (probable calcium-binding protein CML18 OS=Cucurbita moschata OX=3662 GN=LOC111432164 PE=4 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 4.6e-83
Identity = 160/163 (98.16%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60
           MS+KQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA
Sbjct: 1   MSDKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60

Query: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120
           DTNSNGLVEFSEFVALVEPELVSAKCPYTE+QL+QLFRMFDRDGNGFITAAELAHSMAKL
Sbjct: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEDQLRQLFRMFDRDGNGFITAAELAHSMAKL 120

Query: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 164
           GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA
Sbjct: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 163

[Close]

BLAST of CSPI03G01400.1 vs. ExPASy TrEMBL
Match: A0A6J1HK10 (probable calcium-binding protein CML18 OS=Cucurbita maxima OX=3661 GN=LOC111465223 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 6.0e-83
Identity = 160/163 (98.16%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60
           MS+KQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLE+LIMKA
Sbjct: 1   MSDKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLENLIMKA 60

Query: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120
           DTNSNGLVEFSEFVAL EPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL
Sbjct: 61  DTNSNGLVEFSEFVALAEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120

Query: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 164
           GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA
Sbjct: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 163

[Close]

BLAST of CSPI03G01400.1 vs. NCBI nr
Match: XP_004148765.1 (probable calcium-binding protein CML18 [Cucumis sativus] >KGN55728.1 hypothetical protein Csa_009749 [Cucumis sativus])

HSP 1 Score: 321.2 bits (822), Expect = 5.0e-84
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60
           MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA
Sbjct: 1   MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60

Query: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120
           DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL
Sbjct: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120

Query: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 164
           GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA
Sbjct: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 163

[Close]

BLAST of CSPI03G01400.1 vs. NCBI nr
Match: XP_008448654.1 (PREDICTED: probable calcium-binding protein CML18 [Cucumis melo] >XP_038905572.1 probable calcium-binding protein CML18 [Benincasa hispida] >KAA0053024.1 putative calcium-binding protein CML18 [Cucumis melo var. makuwa] >TYK11479.1 putative calcium-binding protein CML18 [Cucumis melo var. makuwa])

HSP 1 Score: 318.2 bits (814), Expect = 4.3e-83
Identity = 161/163 (98.77%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60
           MS+KQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLE+LIMKA
Sbjct: 1   MSDKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLENLIMKA 60

Query: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120
           DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL
Sbjct: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120

Query: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 164
           GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA
Sbjct: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 163

[Close]

BLAST of CSPI03G01400.1 vs. NCBI nr
Match: XP_022924802.1 (probable calcium-binding protein CML18 [Cucurbita moschata] >XP_023540544.1 probable calcium-binding protein CML18 [Cucurbita pepo subsp. pepo] >KAG6596722.1 putative calcium-binding protein CML17, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 317.0 bits (811), Expect = 9.5e-83
Identity = 160/163 (98.16%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60
           MS+KQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA
Sbjct: 1   MSDKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60

Query: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120
           DTNSNGLVEFSEFVALVEPELVSAKCPYTE+QL+QLFRMFDRDGNGFITAAELAHSMAKL
Sbjct: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEDQLRQLFRMFDRDGNGFITAAELAHSMAKL 120

Query: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 164
           GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA
Sbjct: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 163

[Close]

BLAST of CSPI03G01400.1 vs. NCBI nr
Match: XP_022923350.1 (probable calcium-binding protein CML18 [Cucurbita moschata] >XP_022965322.1 probable calcium-binding protein CML18 [Cucurbita maxima] >XP_022965323.1 probable calcium-binding protein CML18 [Cucurbita maxima] >XP_022965324.1 probable calcium-binding protein CML18 [Cucurbita maxima] >XP_023552506.1 probable calcium-binding protein CML18 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 316.6 bits (810), Expect = 1.2e-82
Identity = 160/163 (98.16%), Postives = 162/163 (99.39%), Query Frame = 0


Query: 1   MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60
           MS+KQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLE+LIMKA
Sbjct: 1   MSDKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLENLIMKA 60

Query: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120
           DTNSNGLVEFSEFVAL EPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL
Sbjct: 61  DTNSNGLVEFSEFVALAEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120

Query: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 164
           GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA
Sbjct: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 163

[Close]

BLAST of CSPI03G01400.1 vs. NCBI nr
Match: XP_022145375.1 (probable calcium-binding protein CML18 [Momordica charantia])

HSP 1 Score: 315.8 bits (808), Expect = 2.1e-82
Identity = 159/163 (97.55%), Postives = 163/163 (100.00%), Query Frame = 0


Query: 1   MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60
           MS+KQPIKLDDEQ+AELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLE+LIMKA
Sbjct: 1   MSDKQPIKLDDEQMAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLENLIMKA 60

Query: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKL 120
           DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQL+QLFRMFDRDGNGFITAAELAHSMAKL
Sbjct: 61  DTNSNGLVEFSEFVALVEPELVSAKCPYTEEQLRQLFRMFDRDGNGFITAAELAHSMAKL 120

Query: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 164
           GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA
Sbjct: 121 GHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSWA 163

[Close]

BLAST of CSPI03G01400.1 vs. TAIR 10
Match: AT3G03000.1 (EF hand calcium-binding protein family )

HSP 1 Score: 261.2 bits (666), Expect = 5.8e-70
Identity = 131/157 (83.44%), Postives = 145/157 (92.36%), Query Frame = 0


Query: 6   PIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKADTNSN 65
           P KL DEQ+AELREIFRSFD+N DGSLT+LELGSLLRSLGLKPS DQL++LI KAD N+N
Sbjct: 10  PAKLGDEQLAELREIFRSFDQNKDGSLTELELGSLLRSLGLKPSQDQLDTLIQKADRNNN 69

Query: 66  GLVEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKLGHALT 125
           GLVEFSEFVALVEP+LV  KCPYT++QLK +FRMFDRDGNG+ITAAELAHSMAKLGHALT
Sbjct: 70  GLVEFSEFVALVEPDLV--KCPYTDDQLKAIFRMFDRDGNGYITAAELAHSMAKLGHALT 129

Query: 126 AEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSW 163
           AEELTGMI+EAD DGDG I+FQEF QAITSAAFDN+W
Sbjct: 130 AEELTGMIKEADRDGDGCIDFQEFVQAITSAAFDNAW 164

[Close]

BLAST of CSPI03G01400.1 vs. TAIR 10
Match: AT1G32250.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 250.8 bits (639), Expect = 7.8e-67
Identity = 130/165 (78.79%), Postives = 143/165 (86.67%), Query Frame = 0


Query: 1   MSNKQPIKLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKA 60
           MS+K   KLD+EQI ELREIFRSFDRN DGSLTQLELGSLLR+LG+KPSPDQ E+LI KA
Sbjct: 1   MSHKVSKKLDEEQINELREIFRSFDRNKDGSLTQLELGSLLRALGVKPSPDQFETLIDKA 60

Query: 61  DTNSNGLVEFSEFVALVEPELVS---AKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSM 120
           DT SNGLVEF EFVALV PEL+S      PYTEEQL +LFR+FD DGNGFITAAELAHSM
Sbjct: 61  DTKSNGLVEFPEFVALVSPELLSPAKRTTPYTEEQLLRLFRIFDTDGNGFITAAELAHSM 120

Query: 121 AKLGHALTAEELTGMIREADTDGDGRINFQEFSQAITSAAFDNSW 163
           AKLGHALT  ELTGMI+EAD+DGDGRINFQEF++AI SAAFD+ W
Sbjct: 121 AKLGHALTVAELTGMIKEADSDGDGRINFQEFAKAINSAAFDDIW 165

[Close]

BLAST of CSPI03G01400.1 vs. TAIR 10
Match: AT3G25600.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 157.1 bits (396), Expect = 1.2e-38
Identity = 81/148 (54.73%), Postives = 106/148 (71.62%), Query Frame = 0


Query: 12  EQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKADTNSNGLVEFS 71
           +QI +L++IF  FD + DGSLTQLEL +LLRSLG+KP  DQ+  L+ + D N NG VEF 
Sbjct: 8   DQIKQLKDIFARFDMDKDGSLTQLELAALLRSLGIKPRGDQISLLLNQIDRNGNGSVEFD 67

Query: 72  EFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKLGHALTAEELTG 131
           E V  + P+ ++ +    +EQL ++FR FDRDGNG ITAAELA SMAK+GH LT  ELT 
Sbjct: 68  ELVVAILPD-INEEVLINQEQLMEVFRSFDRDGNGSITAAELAGSMAKMGHPLTYRELTE 127

Query: 132 MIREADTDGDGRINFQEFSQAITSAAFD 160
           M+ EAD++GDG I+F EFS  +  +A D
Sbjct: 128 MMTEADSNGDGVISFNEFSHIMAKSAAD 154

[Close]

BLAST of CSPI03G01400.1 vs. TAIR 10
Match: AT1G18530.1 (EF hand calcium-binding protein family )

HSP 1 Score: 156.4 bits (394), Expect = 2.0e-38
Identity = 81/149 (54.36%), Postives = 108/149 (72.48%), Query Frame = 0


Query: 11  DEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKADTNSNGLVEF 70
           ++QI +L++IF  FD + DGSLT LEL +LLRSLGLKPS DQ+  L+   D+N NG VEF
Sbjct: 2   EDQIRQLKDIFDRFDMDADGSLTILELAALLRSLGLKPSGDQIHVLLASMDSNGNGFVEF 61

Query: 71  SEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKLGHALTAEELT 130
            E V  + P+L + +     EQL ++F+ FDRDGNGFI+AAELA +MAK+G  LT +ELT
Sbjct: 62  DELVGTILPDL-NEEVLINSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELT 121

Query: 131 GMIREADTDGDGRINFQEFSQAITSAAFD 160
            MI+EADT+GDG I+F EF+  +  +A D
Sbjct: 122 EMIKEADTNGDGVISFGEFASIMAKSAVD 149

[Close]

BLAST of CSPI03G01400.1 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 142.1 bits (357), Expect = 3.9e-34
Identity = 70/142 (49.30%), Postives = 101/142 (71.13%), Query Frame = 0


Query: 8   KLDDEQIAELREIFRSFDRNNDGSLTQLELGSLLRSLGLKPSPDQLESLIMKADTNSNGL 67
           +L DEQI+E +E F  FD++ DG +T  ELG+++RSLG  P+  +L+ +I + D + NG 
Sbjct: 4   QLTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGT 63

Query: 68  VEFSEFVALVEPELVSAKCPYTEEQLKQLFRMFDRDGNGFITAAELAHSMAKLGHALTAE 127
           ++F EF+ L+  ++   K   +EE+LK+ FR+FD+D NGFI+AAEL H M  LG  LT E
Sbjct: 64  IDFPEFLNLMAKKM---KDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 123

Query: 128 ELTGMIREADTDGDGRINFQEF 150
           E+  MIREAD DGDG+IN++EF
Sbjct: 124 EVEEMIREADVDGDGQINYEEF 142

The following BLAST results are available for this feature:

BLAST of CSPI03G01400.1 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M8U1	8.1e-69	83.44	Probable calcium-binding protein CML18 OS=Arabidopsis thaliana OX=3702 GN=CML18 ...	[more]
Q9LQN4	1.1e-65	78.79	Probable calcium-binding protein CML17 OS=Arabidopsis thaliana OX=3702 GN=CML17 ...	[more]
Q9AWK2	1.9e-65	78.34	Probable calcium-binding protein CML11 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q6L5F4	7.2e-41	55.19	Probable calcium-binding protein CML14 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9LI84	1.7e-37	54.73	Probable calcium-binding protein CML16 OS=Arabidopsis thaliana OX=3702 GN=CML16 ...	[more]

BLAST of CSPI03G01400.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L1S8	2.4e-84	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G008850 PE=4 SV=1	[more]
A0A5A7UCP8	2.1e-83	98.77	Putative calcium-binding protein CML18 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3BKU3	2.1e-83	98.77	probable calcium-binding protein CML18 OS=Cucumis melo OX=3656 GN=LOC103490757 P...	[more]
A0A6J1EA11	4.6e-83	98.16	probable calcium-binding protein CML18 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1HK10	6.0e-83	98.16	probable calcium-binding protein CML18 OS=Cucurbita maxima OX=3661 GN=LOC1114652...	[more]

BLAST of CSPI03G01400.1 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148765.1	5.0e-84	100.00	probable calcium-binding protein CML18 [Cucumis sativus] >KGN55728.1 hypothetica...	[more]
XP_008448654.1	4.3e-83	98.77	PREDICTED: probable calcium-binding protein CML18 [Cucumis melo] >XP_038905572.1...	[more]
XP_022924802.1	9.5e-83	98.16	probable calcium-binding protein CML18 [Cucurbita moschata] >XP_023540544.1 prob...	[more]
XP_022923350.1	1.2e-82	98.16	probable calcium-binding protein CML18 [Cucurbita moschata] >XP_022965322.1 prob...	[more]
XP_022145375.1	2.1e-82	97.55	probable calcium-binding protein CML18 [Momordica charantia]	[more]

BLAST of CSPI03G01400.1 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G03000.1	5.8e-70	83.44	EF hand calcium-binding protein family	[more]
AT1G32250.1	7.8e-67	78.79	Calcium-binding EF-hand family protein	[more]
AT3G25600.1	1.2e-38	54.73	Calcium-binding EF-hand family protein	[more]
AT1G18530.1	2.0e-38	54.36	EF hand calcium-binding protein family	[more]
AT1G66410.1	3.9e-34	49.30	calmodulin 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 52..80 e-value: 0.48 score: 19.5 coord: 92..120 e-value: 1.2E-5 score: 34.8 coord: 128..156 e-value: 2.7E-6 score: 36.9 coord: 16..44 e-value: 6.4E-5 score: 32.3
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 16..77 e-value: 5.1E-15 score: 55.7 coord: 90..153 e-value: 7.6E-16 score: 58.3
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 12..47 score: 15.043417
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 88..123 score: 15.685013
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 124..159 score: 13.453381
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 48..83 score: 10.217514
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 92..154 e-value: 1.15562E-19 score: 75.6621
None	No IPR available	GENE3D	1.10.238.10		coord: 84..159 e-value: 1.1E-24 score: 88.5 coord: 2..83 e-value: 1.5E-21 score: 78.4
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 5..163
None	No IPR available	PANTHER	PTHR23050:SF400	CALCIUM-BINDING PROTEIN CML18-RELATED	coord: 5..163
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 101..113
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 137..149
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 25..37
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 61..73
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 6..154

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G01400	CSPI03G01400	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G01400.1.cds1	CSPI03G01400.1.cds1	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G01400.1	CSPI03G01400.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050790	regulation of catalytic activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030234	enzyme regulator activity