CSPI02G05040.1 (mRNA) Cucumber (PI 183967) v1

BLAST of CSPI02G05040.1 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 394.4 bits (1012), Expect = 2.3e-108
Identity = 227/523 (43.40%), Postives = 324/523 (61.95%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           MG  A   +L+ Y +  MHF L  AAN +TNF+G+ F+ +LLGG+LSD ++    T +IF
Sbjct: 60  MGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIF 119

Query: 61  GFLEVIALILITVQAYSHDLLP---SPLCPKDC--VKGRIAFVFYTSLYLLAIGSGGVRG 120
           GF+E+   IL++VQA+   L P   +PL  + C   KG  A +F+ +LYL+A+GSG V+ 
Sbjct: 120 GFVELSGFILLSVQAHLPQLKPPKCNPLIDQTCEEAKGFKAMIFFMALYLVALGSGCVKP 179

Query: 121 ALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISA 180
            + A GADQF+Q  PK++K L ++FN    +  +G  + +TL+VWV  +     GF +SA
Sbjct: 180 NMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSA 239

Query: 181 LAALVGFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLH 240
            A  +G I    G  ++R + P +S    +  VIV AI  R+L  P  P  L+      +
Sbjct: 240 AAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVAN 299

Query: 241 MDSNHYKIVHTNQLRFLDKAAIVPKD--IEPQPWNVCSVTQVEEVKIITRMVPIFVSTII 300
                  + HT + RFLDKA I  +D   +  PW +C+VTQVE+VK +  +VPIF STI+
Sbjct: 300 DVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIV 359

Query: 301 MNTCLAQLQTFSVEQG---NTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPF 360
            NT LAQLQTFSV+QG   NTR+ +     F  P  S+  IP + + FL+PLY+ F VPF
Sbjct: 360 FNTILAQLQTFSVQQGSSMNTRLSNS----FHIPPASLQAIPYIMLIFLVPLYDSFLVPF 419

Query: 361 ARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYG 420
           ARK+T H SGI  L R+G+GL LS  +M  A ++E KRR  ++    + +S+FW+  Q+ 
Sbjct: 420 ARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRR-DSSVLDGRILSIFWITPQFL 479

Query: 421 IFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITP 480
           IFG+++MFT VGL+EFFYK++  GM S   + T+ S + G+Y SS+ V+VVNK+T     
Sbjct: 480 IFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVD 539

Query: 481 SKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASW 514
           S KGW   L   DLN + L+LFYW LA+LS+LNF  YL+W+ W
Sbjct: 540 S-KGW---LGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

BLAST of CSPI02G05040.1 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 5.1e-108
Identity = 229/539 (42.49%), Postives = 320/539 (59.37%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           + F+AN ++LV Y    MH  L  +++ +T FM +AFLL+LLGGFL+D + +     LI 
Sbjct: 46  LAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLIS 105

Query: 61  GFLEVIALILITVQAYSHDLLPSPLCPK-----DCVKGRIAFVFYTSLYLLAIGSGGVRG 120
             +E + LIL+T+QA    L+P P         + V G  A   +  LYL+++G GG++G
Sbjct: 106 ASIEFLGLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKG 165

Query: 121 ALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISA 180
           +LP+ GA+QF++  PK  K   TFFNY +  +  GA V VT +VW+  NK W WGF +S 
Sbjct: 166 SLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVST 225

Query: 181 LAALVGFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKL- 240
           ++  +  ++F +G  FY+ ++P  SPL  + +V++ A              +   S K  
Sbjct: 226 ISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAA-------------SIVSCSSKTS 285

Query: 241 --HMDSNHYKIVH---------TNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITR 300
             H  S   +  H         TN L  L+KA    +      W  C+V QVE+VKI+ +
Sbjct: 286 SNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAI---EGKTHHIWLECTVQQVEDVKIVLK 345

Query: 301 MVPIFVSTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLY 360
           M+PIF  TI++N CLAQL T+SV Q  T  M++ + +F  P+ S+PV P+VFM  L P Y
Sbjct: 346 MLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFPVVFMLILAPTY 405

Query: 361 EFFFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATE------HPD 420
           +   +PFARK+T    GIT LQR+GVGLVLS +AM VA LVE+KR+  A E         
Sbjct: 406 DHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEET 465

Query: 421 KQISLFWLAFQYGIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIF 480
             I+  W+A QY   G AD+FTL GLLEFF+ EAP  MRSL+TS ++ SLALGYYLSS+ 
Sbjct: 466 LPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVM 525

Query: 481 VNVVNKVTKNITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKY 517
           V +VN+VTK+   S   W    + E LN N L+LFYW + +LSV+NF HYL+WA  YKY
Sbjct: 526 VPIVNRVTKSAGQSP--W----LGEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of CSPI02G05040.1 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.1e-105
Identity = 225/550 (40.91%), Postives = 324/550 (58.91%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           + ++AN ++LV Y    MH     +AN +TNFMG+AFLL+LLGGFLSD + +     LI 
Sbjct: 44  LAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLIS 103

Query: 61  GFLEVIALILITVQAYSHDLLP----SPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGA 120
             +E + LI++T+QA +  L+P    SP C +  V G  A + +  LYL+A+G GG++G+
Sbjct: 104 ASIEFLGLIILTIQARTPSLMPPSCDSPTCEE--VSGSKAAMLFVGLYLVALGVGGIKGS 163

Query: 121 LPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISAL 180
           L + GA+QF++  PK  K   TFFNY +  +  GA V VT +VW+  NK W WGF +S +
Sbjct: 164 LASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTI 223

Query: 181 AALVGFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVA-IKNRRLRLPDTPNELYEISDKLH 240
           A  V  +IF  G  FYR ++P  SPL  +++V++ A +K               +S   H
Sbjct: 224 AIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPSNH 283

Query: 241 MDSNHYKIVH--------------------TNQLRFLDKAAIVPKDIEP-QPWNVCSVTQ 300
             S   K V                     TN L+ L+ AA    D +P      C+V Q
Sbjct: 284 CVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAA----DEKPVHRLLECTVQQ 343

Query: 301 VEEVKIITRMVPIFVSTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLV 360
           VE+VKI+ +M+PIF  TI++N CLAQL TFSV+Q  +  M+  +G  + P  S+P+ P+V
Sbjct: 344 VEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAAS--MNTKIGSLKIPPASLPIFPVV 403

Query: 361 FMAFLIPLYEFFFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATE 420
           F+  L P+Y+   +PFARK T   +G+T LQR+GVGLVLS +AM VA LVE+KR+  A +
Sbjct: 404 FIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKD 463

Query: 421 ------HPDKQISLFWLAFQYGIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLA 480
                      ++  W+A QY   G AD+FTL GLLE+F+ EAP  MRSL+TS ++ SLA
Sbjct: 464 SGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLA 523

Query: 481 LGYYLSSIFVNVVNKVTKNITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYL 519
           +GYYLSS+ V++VN +T   +     W+ G   + +N   L+ FYW + +LS  NF HYL
Sbjct: 524 MGYYLSSVIVSIVNSITG--SSGNTPWLRG---KSINRYKLDYFYWLMCVLSAANFLHYL 580

BLAST of CSPI02G05040.1 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 2.9e-103
Identity = 226/525 (43.05%), Postives = 317/525 (60.38%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           M   A   +L+ Y    MHF L  +AN +TNF+G+ FLLSLLGGFLSD+Y+    T L+F
Sbjct: 57  MAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVF 116

Query: 61  GFLEVIALILITVQAYSHDLLPSPLCPKD----CVK--GRIAFVFYTSLYLLAIGSGGVR 120
           G +E+   IL++VQA+  +L P     K     CV+  G  A   YT+L L+A+GSG ++
Sbjct: 117 GVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYKAATLYTALCLVALGSGCLK 176

Query: 121 GALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFIS 180
             + + GA+QF +KD    + L +FFN    +  +G  + +TL+VWV  +     GF +S
Sbjct: 177 PNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVS 236

Query: 181 ALAALVGFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKL 240
           A     G I    G  FYR + P  S    + QV V AI  R+   P  PN +++ S  L
Sbjct: 237 AAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRKQICPSNPNMVHQPSTDL 296

Query: 241 HMDSNHYKIVHTNQLRFLDKAAIVPK-DIEPQPWNVCSVTQVEEVKIITRMVPIFVSTII 300
                   ++H+N+ RFLDKA I  +      PW +C++ QV +VKI+  ++PIF  TII
Sbjct: 297 ---VRVKPLLHSNKFRFLDKACIKTQGKAMESPWRLCTIEQVHQVKILLSVIPIFACTII 356

Query: 301 MNTCLAQLQTFSVEQG---NTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPF 360
            NT LAQLQTFSV+QG   NT I       FQ P  S+  IP + + F +PLYE FFVP 
Sbjct: 357 FNTILAQLQTFSVQQGSSMNTHITKT----FQIPPASLQAIPYIILIFFVPLYETFFVPL 416

Query: 361 ARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYG 420
           ARK+T + SGI+ LQR+G GL L+  +M  A LVE KRR    E  +  +S+FW+A Q+ 
Sbjct: 417 ARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQ-NVMLSIFWIAPQFL 476

Query: 421 IFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVT-KNIT 480
           IFG+++MFT VGL+EFFYK++   M+S  T+ T+ S + G+YLSS+ V+ VN+VT  N +
Sbjct: 477 IFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGS 536

Query: 481 PSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWY 515
            +K+GW   L   DLN + L+ FYW LA LS +NFF+YL+W+ WY
Sbjct: 537 GTKEGW---LGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWY 567

BLAST of CSPI02G05040.1 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 6.3e-98
Identity = 204/522 (39.08%), Postives = 310/522 (59.39%), Query Frame = 0

Query: 8   TSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIFGFLEVIA 67
           T+LV Y    ++    TAAN +TN+ G+ ++  L+G F++D Y+ R  T   F F+ V  
Sbjct: 50  TNLVNYLESRLNQGNATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSG 109

Query: 68  LILITVQAYSHDLLPSPLCPKDC-VKGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFN 127
           + L+T+ A    L P       C        VF+ +LY++A+G+GG++  + + GADQF+
Sbjct: 110 MTLLTLSASVPGLKPGNCNADTCHPNSSQTAVFFVALYMIALGTGGIKPCVSSFGADQFD 169

Query: 128 QKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALVGFIIFA 187
           + D  E     +FFN+   S+ +GA +  T++VW+ +N  W WGF +  +A ++    F 
Sbjct: 170 ENDENEKIKKSSFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFF 229

Query: 188 IGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNHYKIVHT 247
            G  FYR+Q PG SPL R+ QVIV A +   +++P+  + L+E +D         K+VHT
Sbjct: 230 FGSRFYRLQRPGGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHT 289

Query: 248 NQLRFLDKAAIVP-----KDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQL 307
           + L+F DKAA+       KD E  PW +CSVTQVEE+K I  ++P++ + I+  T  +Q+
Sbjct: 290 DNLKFFDKAAVESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQM 349

Query: 308 QTFSVEQGNTRIMDKSLG-HFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG 367
            T  V QGNT  MD+ +G +F+ P+ S+ +   V + F  P+Y+ F +P ARK T +  G
Sbjct: 350 STMFVLQGNT--MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERG 409

Query: 368 ITQLQRVGVGLVLSAIAMTVAGLVEVKR-----RHQATEHPDKQISLFWLAFQYGIFGVA 427
            TQLQR+G+GLV+S  AM  AG++EV R      H A +     +S+FW   QY + G A
Sbjct: 410 FTQLQRMGIGLVVSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCA 469

Query: 428 DMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGW 487
           ++FT +G LEFFY +AP  MRSL ++ +  ++ALG YLS++ V VV K+TK     K GW
Sbjct: 470 EVFTFIGQLEFFYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKK--NGKPGW 529

Query: 488 VEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYK 518
               IP++LN  +L+ F++ LA LS LNF  YL+ +  YKYK
Sbjct: 530 ----IPDNLNRGHLDYFFYLLATLSFLNFLVYLWISKRYKYK 563

BLAST of CSPI02G05040.1 vs. ExPASy TrEMBL
Match: A0A0A0LM59 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G035360 PE=3 SV=1)

HSP 1 Score: 1198.0 bits (3098), Expect = 0.0e+00
Identity = 611/612 (99.84%), Postives = 612/612 (100.00%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF
Sbjct: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60

Query: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120
           GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL
Sbjct: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120

Query: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV 180
           GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV
Sbjct: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV 180

Query: 181 GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH 240
           GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH
Sbjct: 181 GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH 240

Query: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ 300
           YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ
Sbjct: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ 300

Query: 301 LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG 360
           LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG
Sbjct: 301 LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG 360

Query: 361 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL 420
           ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL
Sbjct: 361 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL 420

Query: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI 480
           VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI
Sbjct: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI 480

Query: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGETTKEPIAEL 540
           PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGETTKEPIAEL
Sbjct: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGETTKEPIAEL 540

Query: 541 NKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITESNERGGDDVPILHKEGN 600
           NKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITESNERGGDDVPILHKEGN
Sbjct: 541 NKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITESNERGGDDVPILHKEGN 600

Query: 601 GEDAKTHHSEEK 613
           GEDAKTHH+EEK
Sbjct: 601 GEDAKTHHTEEK 612

BLAST of CSPI02G05040.1 vs. ExPASy TrEMBL
Match: A0A1S4E0E0 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucumis melo OX=3656 GN=LOC103493841 PE=3 SV=1)

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 585/628 (93.15%), Postives = 599/628 (95.38%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           MGFVANMTSLVQYFLFVMHFDL+TAANTLTNFMGSAFLLSLLGGFLSDTYINRL TCLIF
Sbjct: 51  MGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIF 110

Query: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120
           GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL
Sbjct: 111 GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 170

Query: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV 180
           GADQFNQKDPKEAKALGTFFNYMLLSVV+GAAVGVT+IVWVAVNKAWYWGFFISALAALV
Sbjct: 171 GADQFNQKDPKEAKALGTFFNYMLLSVVLGAAVGVTVIVWVAVNKAWYWGFFISALAALV 230

Query: 181 GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH 240
           GFIIFAIGKPFYRIQVPGQSP+LRVIQVIVVAIKNRRLRLPDTPNELYEISDK++MDS H
Sbjct: 231 GFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIH 290

Query: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ 300
           YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIF+STIIMNTCLAQ
Sbjct: 291 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQ 350

Query: 301 LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG 360
           LQTFSVEQGNT IMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEF FVPFARKITHHPSG
Sbjct: 351 LQTFSVEQGNTIIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG 410

Query: 361 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL 420
           ITQLQRVGVGLVLSAIAM VAGLVEVKRRHQA +HPD+ ISLFWLAFQYGIFG+ADMFTL
Sbjct: 411 ITQLQRVGVGLVLSAIAMAVAGLVEVKRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTL 470

Query: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI 480
           VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVN+VNKVT+NITPSK+GWVEGLI
Sbjct: 471 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLI 530

Query: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGET-------- 540
           PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEP+ ELNSKGGET        
Sbjct: 531 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPIEELNSKGGETTKEPIAEL 590

Query: 541 --------TKEPIAELNKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITES 600
                   TKEPIAELNKQGEEIKEPT    LNGRGEGETKEPN ELNGGRETKEPITES
Sbjct: 591 NSKEGETMTKEPIAELNKQGEEIKEPTAE--LNGRGEGETKEPNVELNGGRETKEPITES 650

Query: 601 NERGGDDVPILHKEGNGEDAKTHHSEEK 613
           NERGGDDVPILHKEGNGEDAKTHHSEEK
Sbjct: 651 NERGGDDVPILHKEGNGEDAKTHHSEEK 676

BLAST of CSPI02G05040.1 vs. ExPASy TrEMBL
Match: A0A5D3BBU1 (Protein NRT1/ PTR FAMILY 4.5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G00540 PE=3 SV=1)

HSP 1 Score: 1139.0 bits (2945), Expect = 0.0e+00
Identity = 584/628 (92.99%), Postives = 599/628 (95.38%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           MGFVANMTSLVQYFLFVMHFDL+TAANTLTNFMGSAFLLSLLGGFLSDTYINRL TCLIF
Sbjct: 1   MGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIF 60

Query: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120
           GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL
Sbjct: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120

Query: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV 180
           GADQFNQKDPKEAKALGTFFNYMLLSVV+GAAVGVT+IVWVAVNKAWYWGFFISALAALV
Sbjct: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVLGAAVGVTVIVWVAVNKAWYWGFFISALAALV 180

Query: 181 GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH 240
           GFIIFAIGKPFYRIQVPGQSP+LRVIQVIVVAIKNRRLRLPDTPNELYEISDK++MDS H
Sbjct: 181 GFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIH 240

Query: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ 300
           YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIF+STIIMNTCLAQ
Sbjct: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQ 300

Query: 301 LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG 360
           LQTFSVEQGNT IMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEF FVPFARKITHHPSG
Sbjct: 301 LQTFSVEQGNTIIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG 360

Query: 361 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL 420
           ITQLQRVGVGLVLSAIAM VAGLVEVKRRHQA +HPD+ ISLFWLAFQYGIFG+ADMFTL
Sbjct: 361 ITQLQRVGVGLVLSAIAMAVAGLVEVKRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTL 420

Query: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI 480
           VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVN+VNKVT+NITPSK+GWVEGLI
Sbjct: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLI 480

Query: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGET-------- 540
           PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEP+ ELNS+GGET        
Sbjct: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPIEELNSRGGETTKEPIAEL 540

Query: 541 --------TKEPIAELNKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITES 600
                   TKEPIAELNKQGEEIKEPT    LNGRGEGETKEPN ELNGGRETKEPITES
Sbjct: 541 NSKEGETMTKEPIAELNKQGEEIKEPTAE--LNGRGEGETKEPNVELNGGRETKEPITES 600

Query: 601 NERGGDDVPILHKEGNGEDAKTHHSEEK 613
           NERGGDDVPILHKEGNGEDAKTHHSEEK
Sbjct: 601 NERGGDDVPILHKEGNGEDAKTHHSEEK 626

BLAST of CSPI02G05040.1 vs. ExPASy TrEMBL
Match: A0A5A7ULM1 (Protein NRT1/ PTR FAMILY 4.5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold323G00540 PE=3 SV=1)

HSP 1 Score: 1139.0 bits (2945), Expect = 0.0e+00
Identity = 584/628 (92.99%), Postives = 599/628 (95.38%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           MGFVANMTSLVQYFLFVMHFDL+TAANTLTNFMGSAFLLSLLGGFLSDTYINRL TCLIF
Sbjct: 36  MGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIF 95

Query: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120
           GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL
Sbjct: 96  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 155

Query: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV 180
           GADQFNQKDPKEAKALGTFFNYMLLSVV+GAAVGVT+IVWVAVNKAWYWGFFISALAALV
Sbjct: 156 GADQFNQKDPKEAKALGTFFNYMLLSVVLGAAVGVTVIVWVAVNKAWYWGFFISALAALV 215

Query: 181 GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH 240
           GFIIFAIGKPFYRIQVPGQSP+LRVIQVIVVAIKNRRLRLPDTPNELYEISDK++MDS H
Sbjct: 216 GFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIH 275

Query: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ 300
           YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIF+STIIMNTCLAQ
Sbjct: 276 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQ 335

Query: 301 LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG 360
           LQTFSVEQGNT IMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEF FVPFARKITHHPSG
Sbjct: 336 LQTFSVEQGNTIIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG 395

Query: 361 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL 420
           ITQLQRVGVGLVLSAIAM VAGLVEVKRRHQA +HPD+ ISLFWLAFQYGIFG+ADMFTL
Sbjct: 396 ITQLQRVGVGLVLSAIAMAVAGLVEVKRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTL 455

Query: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI 480
           VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVN+VNKVT+NITPSK+GWVEGLI
Sbjct: 456 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLI 515

Query: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGET-------- 540
           PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEP+ ELNS+GGET        
Sbjct: 516 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPIEELNSRGGETTKEPIAEL 575

Query: 541 --------TKEPIAELNKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITES 600
                   TKEPIAELNKQGEEIKEPT    LNGRGEGETKEPN ELNGGRETKEPITES
Sbjct: 576 NSKEGETMTKEPIAELNKQGEEIKEPTAE--LNGRGEGETKEPNVELNGGRETKEPITES 635

Query: 601 NERGGDDVPILHKEGNGEDAKTHHSEEK 613
           NERGGDDVPILHKEGNGEDAKTHHSEEK
Sbjct: 636 NERGGDDVPILHKEGNGEDAKTHHSEEK 661

BLAST of CSPI02G05040.1 vs. ExPASy TrEMBL
Match: A0A6J1FIK0 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111444459 PE=3 SV=1)

HSP 1 Score: 930.2 bits (2403), Expect = 4.3e-267
Identity = 478/612 (78.10%), Postives = 521/612 (85.13%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           MGFVANM SLVQYFL V+HFDLQTAANTLTNF+GSAFLLSLLGGFLSDTYINRL TCLIF
Sbjct: 37  MGFVANMVSLVQYFLMVLHFDLQTAANTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIF 96

Query: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120
           G LEV+AL+++TVQAYSHDLLP   CPKDCV+GRIAFVFYTSLYLLAIGSGGVRGALPAL
Sbjct: 97  GVLEVLALVIVTVQAYSHDLLPKADCPKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPAL 156

Query: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV 180
           GADQFNQKDPKEAKAL TFFN +LLSVVIGAAVGVT+IVWVAVNKAWYWGFFISALA  V
Sbjct: 157 GADQFNQKDPKEAKALATFFNCLLLSVVIGAAVGVTMIVWVAVNKAWYWGFFISALATAV 216

Query: 181 GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH 240
           GFI+FA+GKPFYR+ VPGQSPLLR+IQVIVVA+KNR L LPDTPNELYE+SDK +MDS  
Sbjct: 217 GFIVFALGKPFYRLHVPGQSPLLRIIQVIVVAVKNRGLALPDTPNELYELSDKYYMDSID 276

Query: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ 300
            KI HTNQLR LDKA+I+PKD+EPQPW VCSVTQVEEVKIITRM+PIF+STIIMNTCLAQ
Sbjct: 277 SKIAHTNQLRCLDKASILPKDVEPQPWKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQ 336

Query: 301 LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG 360
           LQTFSVEQGNT+IMDKSLGH QFPAPSIPVIPLVFMAFLIPLYEF FVPFARKITHHPSG
Sbjct: 337 LQTFSVEQGNTQIMDKSLGHLQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG 396

Query: 361 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL 420
           ITQLQRVGVGLVLSA++M VAGLVEVKRRHQAT+HPD+ +SLFWL+FQYGIFG+ADMFTL
Sbjct: 397 ITQLQRVGVGLVLSAVSMAVAGLVEVKRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTL 456

Query: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI 480
           VGLLEFFYKEAPVGMRSLSTSFT+LSL+ GYYLSSIFVNV+NK+T+ ++PSKKGWVEGLI
Sbjct: 457 VGLLEFFYKEAPVGMRSLSTSFTWLSLSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLI 516

Query: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGETTKEPIAEL 540
           PEDLN+NNLNLFYWFLAILS+LNFFHYLYWASWYKYKTEE +V+L+  GG          
Sbjct: 517 PEDLNYNNLNLFYWFLAILSLLNFFHYLYWASWYKYKTEESIVKLDDGGG---------- 576

Query: 541 NKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITESNERGGDDVPILHKEGN 600
                              GE E           RE   PI    ER G D+   H E  
Sbjct: 577 -------------------GERE-----------REEGVPILREEERSGGDIKCHHSE-E 607

Query: 601 GEDAKTHHSEEK 613
            EDA   H EE+
Sbjct: 637 KEDAPIFHEEEE 607

BLAST of CSPI02G05040.1 vs. NCBI nr
Match: KGN61036.1 (hypothetical protein Csa_021307 [Cucumis sativus])

HSP 1 Score: 1198.0 bits (3098), Expect = 0.0e+00
Identity = 611/612 (99.84%), Postives = 612/612 (100.00%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF
Sbjct: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60

Query: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120
           GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL
Sbjct: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120

Query: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV 180
           GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV
Sbjct: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV 180

Query: 181 GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH 240
           GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH
Sbjct: 181 GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH 240

Query: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ 300
           YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ
Sbjct: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ 300

Query: 301 LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG 360
           LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG
Sbjct: 301 LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG 360

Query: 361 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL 420
           ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL
Sbjct: 361 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL 420

Query: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI 480
           VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI
Sbjct: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI 480

Query: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGETTKEPIAEL 540
           PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGETTKEPIAEL
Sbjct: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGETTKEPIAEL 540

Query: 541 NKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITESNERGGDDVPILHKEGN 600
           NKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITESNERGGDDVPILHKEGN
Sbjct: 541 NKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITESNERGGDDVPILHKEGN 600

Query: 601 GEDAKTHHSEEK 613
           GEDAKTHH+EEK
Sbjct: 601 GEDAKTHHTEEK 612

BLAST of CSPI02G05040.1 vs. NCBI nr
Match: XP_031736687.1 (protein NRT1/ PTR FAMILY 4.5 [Cucumis sativus])

HSP 1 Score: 1176.8 bits (3043), Expect = 0.0e+00
Identity = 605/619 (97.74%), Postives = 606/619 (97.90%), Query Frame = 0

Query: 7   MTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIFGFLEVI 66
           MTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIFGFLEVI
Sbjct: 1   MTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIFGFLEVI 60

Query: 67  -------------ALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGV 126
                        ALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGV
Sbjct: 61  FHIYCIKRYEYTQALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGV 120

Query: 127 RGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFI 186
           RGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFI
Sbjct: 121 RGALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFI 180

Query: 187 SALAALVGFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDK 246
           SALAALVGFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDK
Sbjct: 181 SALAALVGFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDK 240

Query: 247 LHMDSNHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTII 306
           LHMDSNHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTII
Sbjct: 241 LHMDSNHYKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTII 300

Query: 307 MNTCLAQLQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARK 366
           MNTCLAQLQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARK
Sbjct: 301 MNTCLAQLQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARK 360

Query: 367 ITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFG 426
           ITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFG
Sbjct: 361 ITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFG 420

Query: 427 VADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKK 486
           VADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKK
Sbjct: 421 VADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKK 480

Query: 487 GWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGETT 546
           GWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGETT
Sbjct: 481 GWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGETT 540

Query: 547 KEPIAELNKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITESNERGGDDVP 606
           KEPIAELNKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITESNERGGDDVP
Sbjct: 541 KEPIAELNKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITESNERGGDDVP 600

Query: 607 ILHKEGNGEDAKTHHSEEK 613
           ILHKEGNGEDAKTHH+EEK
Sbjct: 601 ILHKEGNGEDAKTHHTEEK 619

BLAST of CSPI02G05040.1 vs. NCBI nr
Match: XP_016901445.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.5-like [Cucumis melo])

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 585/628 (93.15%), Postives = 599/628 (95.38%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           MGFVANMTSLVQYFLFVMHFDL+TAANTLTNFMGSAFLLSLLGGFLSDTYINRL TCLIF
Sbjct: 51  MGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIF 110

Query: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120
           GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL
Sbjct: 111 GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 170

Query: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV 180
           GADQFNQKDPKEAKALGTFFNYMLLSVV+GAAVGVT+IVWVAVNKAWYWGFFISALAALV
Sbjct: 171 GADQFNQKDPKEAKALGTFFNYMLLSVVLGAAVGVTVIVWVAVNKAWYWGFFISALAALV 230

Query: 181 GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH 240
           GFIIFAIGKPFYRIQVPGQSP+LRVIQVIVVAIKNRRLRLPDTPNELYEISDK++MDS H
Sbjct: 231 GFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIH 290

Query: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ 300
           YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIF+STIIMNTCLAQ
Sbjct: 291 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQ 350

Query: 301 LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG 360
           LQTFSVEQGNT IMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEF FVPFARKITHHPSG
Sbjct: 351 LQTFSVEQGNTIIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG 410

Query: 361 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL 420
           ITQLQRVGVGLVLSAIAM VAGLVEVKRRHQA +HPD+ ISLFWLAFQYGIFG+ADMFTL
Sbjct: 411 ITQLQRVGVGLVLSAIAMAVAGLVEVKRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTL 470

Query: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI 480
           VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVN+VNKVT+NITPSK+GWVEGLI
Sbjct: 471 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLI 530

Query: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGET-------- 540
           PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEP+ ELNSKGGET        
Sbjct: 531 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPIEELNSKGGETTKEPIAEL 590

Query: 541 --------TKEPIAELNKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITES 600
                   TKEPIAELNKQGEEIKEPT    LNGRGEGETKEPN ELNGGRETKEPITES
Sbjct: 591 NSKEGETMTKEPIAELNKQGEEIKEPTAE--LNGRGEGETKEPNVELNGGRETKEPITES 650

Query: 601 NERGGDDVPILHKEGNGEDAKTHHSEEK 613
           NERGGDDVPILHKEGNGEDAKTHHSEEK
Sbjct: 651 NERGGDDVPILHKEGNGEDAKTHHSEEK 676

BLAST of CSPI02G05040.1 vs. NCBI nr
Match: KAA0056094.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucumis melo var. makuwa])

HSP 1 Score: 1139.0 bits (2945), Expect = 0.0e+00
Identity = 584/628 (92.99%), Postives = 599/628 (95.38%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           MGFVANMTSLVQYFLFVMHFDL+TAANTLTNFMGSAFLLSLLGGFLSDTYINRL TCLIF
Sbjct: 36  MGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIF 95

Query: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120
           GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL
Sbjct: 96  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 155

Query: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV 180
           GADQFNQKDPKEAKALGTFFNYMLLSVV+GAAVGVT+IVWVAVNKAWYWGFFISALAALV
Sbjct: 156 GADQFNQKDPKEAKALGTFFNYMLLSVVLGAAVGVTVIVWVAVNKAWYWGFFISALAALV 215

Query: 181 GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH 240
           GFIIFAIGKPFYRIQVPGQSP+LRVIQVIVVAIKNRRLRLPDTPNELYEISDK++MDS H
Sbjct: 216 GFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIH 275

Query: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ 300
           YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIF+STIIMNTCLAQ
Sbjct: 276 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQ 335

Query: 301 LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG 360
           LQTFSVEQGNT IMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEF FVPFARKITHHPSG
Sbjct: 336 LQTFSVEQGNTIIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG 395

Query: 361 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL 420
           ITQLQRVGVGLVLSAIAM VAGLVEVKRRHQA +HPD+ ISLFWLAFQYGIFG+ADMFTL
Sbjct: 396 ITQLQRVGVGLVLSAIAMAVAGLVEVKRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTL 455

Query: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI 480
           VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVN+VNKVT+NITPSK+GWVEGLI
Sbjct: 456 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLI 515

Query: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGET-------- 540
           PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEP+ ELNS+GGET        
Sbjct: 516 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPIEELNSRGGETTKEPIAEL 575

Query: 541 --------TKEPIAELNKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITES 600
                   TKEPIAELNKQGEEIKEPT    LNGRGEGETKEPN ELNGGRETKEPITES
Sbjct: 576 NSKEGETMTKEPIAELNKQGEEIKEPTAE--LNGRGEGETKEPNVELNGGRETKEPITES 635

Query: 601 NERGGDDVPILHKEGNGEDAKTHHSEEK 613
           NERGGDDVPILHKEGNGEDAKTHHSEEK
Sbjct: 636 NERGGDDVPILHKEGNGEDAKTHHSEEK 661

BLAST of CSPI02G05040.1 vs. NCBI nr
Match: TYJ96424.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucumis melo var. makuwa])

HSP 1 Score: 1139.0 bits (2945), Expect = 0.0e+00
Identity = 584/628 (92.99%), Postives = 599/628 (95.38%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           MGFVANMTSLVQYFLFVMHFDL+TAANTLTNFMGSAFLLSLLGGFLSDTYINRL TCLIF
Sbjct: 1   MGFVANMTSLVQYFLFVMHFDLETAANTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIF 60

Query: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120
           GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL
Sbjct: 61  GFLEVIALILITVQAYSHDLLPSPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPAL 120

Query: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISALAALV 180
           GADQFNQKDPKEAKALGTFFNYMLLSVV+GAAVGVT+IVWVAVNKAWYWGFFISALAALV
Sbjct: 121 GADQFNQKDPKEAKALGTFFNYMLLSVVLGAAVGVTVIVWVAVNKAWYWGFFISALAALV 180

Query: 181 GFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLHMDSNH 240
           GFIIFAIGKPFYRIQVPGQSP+LRVIQVIVVAIKNRRLRLPDTPNELYEISDK++MDS H
Sbjct: 181 GFIIFAIGKPFYRIQVPGQSPILRVIQVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIH 240

Query: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFVSTIIMNTCLAQ 300
           YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIF+STIIMNTCLAQ
Sbjct: 241 YKIVHTNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQ 300

Query: 301 LQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPFARKITHHPSG 360
           LQTFSVEQGNT IMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEF FVPFARKITHHPSG
Sbjct: 301 LQTFSVEQGNTIIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSG 360

Query: 361 ITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYGIFGVADMFTL 420
           ITQLQRVGVGLVLSAIAM VAGLVEVKRRHQA +HPD+ ISLFWLAFQYGIFG+ADMFTL
Sbjct: 361 ITQLQRVGVGLVLSAIAMAVAGLVEVKRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTL 420

Query: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITPSKKGWVEGLI 480
           VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVN+VNKVT+NITPSK+GWVEGLI
Sbjct: 421 VGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLI 480

Query: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPVVELNSKGGET-------- 540
           PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEP+ ELNS+GGET        
Sbjct: 481 PEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKYKTEEPIEELNSRGGETTKEPIAEL 540

Query: 541 --------TKEPIAELNKQGEEIKEPTTAELLNGRGEGETKEPNAELNGGRETKEPITES 600
                   TKEPIAELNKQGEEIKEPT    LNGRGEGETKEPN ELNGGRETKEPITES
Sbjct: 541 NSKEGETMTKEPIAELNKQGEEIKEPTAE--LNGRGEGETKEPNVELNGGRETKEPITES 600

Query: 601 NERGGDDVPILHKEGNGEDAKTHHSEEK 613
           NERGGDDVPILHKEGNGEDAKTHHSEEK
Sbjct: 601 NERGGDDVPILHKEGNGEDAKTHHSEEK 626

BLAST of CSPI02G05040.1 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 394.4 bits (1012), Expect = 1.6e-109
Identity = 227/523 (43.40%), Postives = 324/523 (61.95%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           MG  A   +L+ Y +  MHF L  AAN +TNF+G+ F+ +LLGG+LSD ++    T +IF
Sbjct: 60  MGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIF 119

Query: 61  GFLEVIALILITVQAYSHDLLP---SPLCPKDC--VKGRIAFVFYTSLYLLAIGSGGVRG 120
           GF+E+   IL++VQA+   L P   +PL  + C   KG  A +F+ +LYL+A+GSG V+ 
Sbjct: 120 GFVELSGFILLSVQAHLPQLKPPKCNPLIDQTCEEAKGFKAMIFFMALYLVALGSGCVKP 179

Query: 121 ALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISA 180
            + A GADQF+Q  PK++K L ++FN    +  +G  + +TL+VWV  +     GF +SA
Sbjct: 180 NMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSA 239

Query: 181 LAALVGFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKLH 240
            A  +G I    G  ++R + P +S    +  VIV AI  R+L  P  P  L+      +
Sbjct: 240 AAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKLASPSDPRMLHGDHHVAN 299

Query: 241 MDSNHYKIVHTNQLRFLDKAAIVPKD--IEPQPWNVCSVTQVEEVKIITRMVPIFVSTII 300
                  + HT + RFLDKA I  +D   +  PW +C+VTQVE+VK +  +VPIF STI+
Sbjct: 300 DVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVEQVKTLISLVPIFASTIV 359

Query: 301 MNTCLAQLQTFSVEQG---NTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPF 360
            NT LAQLQTFSV+QG   NTR+ +     F  P  S+  IP + + FL+PLY+ F VPF
Sbjct: 360 FNTILAQLQTFSVQQGSSMNTRLSNS----FHIPPASLQAIPYIMLIFLVPLYDSFLVPF 419

Query: 361 ARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYG 420
           ARK+T H SGI  L R+G+GL LS  +M  A ++E KRR  ++    + +S+FW+  Q+ 
Sbjct: 420 ARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRR-DSSVLDGRILSIFWITPQFL 479

Query: 421 IFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVTKNITP 480
           IFG+++MFT VGL+EFFYK++  GM S   + T+ S + G+Y SS+ V+VVNK+T     
Sbjct: 480 IFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSVLVSVVNKITSTSVD 539

Query: 481 SKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASW 514
           S KGW   L   DLN + L+LFYW LA+LS+LNF  YL+W+ W
Sbjct: 540 S-KGW---LGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

BLAST of CSPI02G05040.1 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 393.3 bits (1009), Expect = 3.6e-109
Identity = 229/539 (42.49%), Postives = 320/539 (59.37%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           + F+AN ++LV Y    MH  L  +++ +T FM +AFLL+LLGGFL+D + +     LI 
Sbjct: 42  LAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLIS 101

Query: 61  GFLEVIALILITVQAYSHDLLPSPLCPK-----DCVKGRIAFVFYTSLYLLAIGSGGVRG 120
             +E + LIL+T+QA    L+P P         + V G  A   +  LYL+++G GG++G
Sbjct: 102 ASIEFLGLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKG 161

Query: 121 ALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISA 180
           +LP+ GA+QF++  PK  K   TFFNY +  +  GA V VT +VW+  NK W WGF +S 
Sbjct: 162 SLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVST 221

Query: 181 LAALVGFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKL- 240
           ++  +  ++F +G  FY+ ++P  SPL  + +V++ A              +   S K  
Sbjct: 222 ISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAA-------------SIVSCSSKTS 281

Query: 241 --HMDSNHYKIVH---------TNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITR 300
             H  S   +  H         TN L  L+KA    +      W  C+V QVE+VKI+ +
Sbjct: 282 SNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAI---EGKTHHIWLECTVQQVEDVKIVLK 341

Query: 301 MVPIFVSTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLY 360
           M+PIF  TI++N CLAQL T+SV Q  T  M++ + +F  P+ S+PV P+VFM  L P Y
Sbjct: 342 MLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFPVVFMLILAPTY 401

Query: 361 EFFFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATE------HPD 420
           +   +PFARK+T    GIT LQR+GVGLVLS +AM VA LVE+KR+  A E         
Sbjct: 402 DHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEET 461

Query: 421 KQISLFWLAFQYGIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIF 480
             I+  W+A QY   G AD+FTL GLLEFF+ EAP  MRSL+TS ++ SLALGYYLSS+ 
Sbjct: 462 LPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVM 521

Query: 481 VNVVNKVTKNITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKY 517
           V +VN+VTK+   S   W    + E LN N L+LFYW + +LSV+NF HYL+WA  YKY
Sbjct: 522 VPIVNRVTKSAGQSP--W----LGEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 556

BLAST of CSPI02G05040.1 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 393.3 bits (1009), Expect = 3.6e-109
Identity = 229/539 (42.49%), Postives = 320/539 (59.37%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           + F+AN ++LV Y    MH  L  +++ +T FM +AFLL+LLGGFL+D + +     LI 
Sbjct: 46  LAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLGGFLADAFFSTFVIFLIS 105

Query: 61  GFLEVIALILITVQAYSHDLLPSPLCPK-----DCVKGRIAFVFYTSLYLLAIGSGGVRG 120
             +E + LIL+T+QA    L+P P         + V G  A   +  LYL+++G GG++G
Sbjct: 106 ASIEFLGLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKG 165

Query: 121 ALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISA 180
           +LP+ GA+QF++  PK  K   TFFNY +  +  GA V VT +VW+  NK W WGF +S 
Sbjct: 166 SLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVST 225

Query: 181 LAALVGFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKL- 240
           ++  +  ++F +G  FY+ ++P  SPL  + +V++ A              +   S K  
Sbjct: 226 ISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAA-------------SIVSCSSKTS 285

Query: 241 --HMDSNHYKIVH---------TNQLRFLDKAAIVPKDIEPQPWNVCSVTQVEEVKIITR 300
             H  S   +  H         TN L  L+KA    +      W  C+V QVE+VKI+ +
Sbjct: 286 SNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAI---EGKTHHIWLECTVQQVEDVKIVLK 345

Query: 301 MVPIFVSTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLY 360
           M+PIF  TI++N CLAQL T+SV Q  T  M++ + +F  P+ S+PV P+VFM  L P Y
Sbjct: 346 MLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFPVVFMLILAPTY 405

Query: 361 EFFFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATE------HPD 420
           +   +PFARK+T    GIT LQR+GVGLVLS +AM VA LVE+KR+  A E         
Sbjct: 406 DHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEET 465

Query: 421 KQISLFWLAFQYGIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIF 480
             I+  W+A QY   G AD+FTL GLLEFF+ EAP  MRSL+TS ++ SLALGYYLSS+ 
Sbjct: 466 LPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVM 525

Query: 481 VNVVNKVTKNITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWYKY 517
           V +VN+VTK+   S   W    + E LN N L+LFYW + +LSV+NF HYL+WA  YKY
Sbjct: 526 VPIVNRVTKSAGQSP--W----LGEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of CSPI02G05040.1 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 385.6 bits (989), Expect = 7.6e-107
Identity = 225/550 (40.91%), Postives = 324/550 (58.91%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           + ++AN ++LV Y    MH     +AN +TNFMG+AFLL+LLGGFLSD + +     LI 
Sbjct: 44  LAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLIS 103

Query: 61  GFLEVIALILITVQAYSHDLLP----SPLCPKDCVKGRIAFVFYTSLYLLAIGSGGVRGA 120
             +E + LI++T+QA +  L+P    SP C +  V G  A + +  LYL+A+G GG++G+
Sbjct: 104 ASIEFLGLIILTIQARTPSLMPPSCDSPTCEE--VSGSKAAMLFVGLYLVALGVGGIKGS 163

Query: 121 LPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFISAL 180
           L + GA+QF++  PK  K   TFFNY +  +  GA V VT +VW+  NK W WGF +S +
Sbjct: 164 LASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTI 223

Query: 181 AALVGFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVA-IKNRRLRLPDTPNELYEISDKLH 240
           A  V  +IF  G  FYR ++P  SPL  +++V++ A +K               +S   H
Sbjct: 224 AIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPSNH 283

Query: 241 MDSNHYKIVH--------------------TNQLRFLDKAAIVPKDIEP-QPWNVCSVTQ 300
             S   K V                     TN L+ L+ AA    D +P      C+V Q
Sbjct: 284 CVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAA----DEKPVHRLLECTVQQ 343

Query: 301 VEEVKIITRMVPIFVSTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHFQFPAPSIPVIPLV 360
           VE+VKI+ +M+PIF  TI++N CLAQL TFSV+Q  +  M+  +G  + P  S+P+ P+V
Sbjct: 344 VEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAAS--MNTKIGSLKIPPASLPIFPVV 403

Query: 361 FMAFLIPLYEFFFVPFARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATE 420
           F+  L P+Y+   +PFARK T   +G+T LQR+GVGLVLS +AM VA LVE+KR+  A +
Sbjct: 404 FIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKD 463

Query: 421 ------HPDKQISLFWLAFQYGIFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLA 480
                      ++  W+A QY   G AD+FTL GLLE+F+ EAP  MRSL+TS ++ SLA
Sbjct: 464 SGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLA 523

Query: 481 LGYYLSSIFVNVVNKVTKNITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYL 519
           +GYYLSS+ V++VN +T   +     W+ G   + +N   L+ FYW + +LS  NF HYL
Sbjct: 524 MGYYLSSVIVSIVNSITG--SSGNTPWLRG---KSINRYKLDYFYWLMCVLSAANFLHYL 580

BLAST of CSPI02G05040.1 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 377.5 bits (968), Expect = 2.1e-104
Identity = 226/525 (43.05%), Postives = 317/525 (60.38%), Query Frame = 0

Query: 1   MGFVANMTSLVQYFLFVMHFDLQTAANTLTNFMGSAFLLSLLGGFLSDTYINRLNTCLIF 60
           M   A   +L+ Y    MHF L  +AN +TNF+G+ FLLSLLGGFLSD+Y+    T L+F
Sbjct: 57  MAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSLLGGFLSDSYLGSFRTMLVF 116

Query: 61  GFLEVIALILITVQAYSHDLLPSPLCPKD----CVK--GRIAFVFYTSLYLLAIGSGGVR 120
           G +E+   IL++VQA+  +L P     K     CV+  G  A   YT+L L+A+GSG ++
Sbjct: 117 GVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYKAATLYTALCLVALGSGCLK 176

Query: 121 GALPALGADQFNQKDPKEAKALGTFFNYMLLSVVIGAAVGVTLIVWVAVNKAWYWGFFIS 180
             + + GA+QF +KD    + L +FFN    +  +G  + +TL+VWV  +     GF +S
Sbjct: 177 PNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIALTLLVWVQTHSGMDVGFGVS 236

Query: 181 ALAALVGFIIFAIGKPFYRIQVPGQSPLLRVIQVIVVAIKNRRLRLPDTPNELYEISDKL 240
           A     G I    G  FYR + P  S    + QV V AI  R+   P  PN +++ S  L
Sbjct: 237 AAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITKRKQICPSNPNMVHQPSTDL 296

Query: 241 HMDSNHYKIVHTNQLRFLDKAAIVPK-DIEPQPWNVCSVTQVEEVKIITRMVPIFVSTII 300
                   ++H+N+ RFLDKA I  +      PW +C++ QV +VKI+  ++PIF  TII
Sbjct: 297 ---VRVKPLLHSNKFRFLDKACIKTQGKAMESPWRLCTIEQVHQVKILLSVIPIFACTII 356

Query: 301 MNTCLAQLQTFSVEQG---NTRIMDKSLGHFQFPAPSIPVIPLVFMAFLIPLYEFFFVPF 360
            NT LAQLQTFSV+QG   NT I       FQ P  S+  IP + + F +PLYE FFVP 
Sbjct: 357 FNTILAQLQTFSVQQGSSMNTHITKT----FQIPPASLQAIPYIILIFFVPLYETFFVPL 416

Query: 361 ARKITHHPSGITQLQRVGVGLVLSAIAMTVAGLVEVKRRHQATEHPDKQISLFWLAFQYG 420
           ARK+T + SGI+ LQR+G GL L+  +M  A LVE KRR    E  +  +S+FW+A Q+ 
Sbjct: 417 ARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQ-NVMLSIFWIAPQFL 476

Query: 421 IFGVADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLSLALGYYLSSIFVNVVNKVT-KNIT 480
           IFG+++MFT VGL+EFFYK++   M+S  T+ T+ S + G+YLSS+ V+ VN+VT  N +
Sbjct: 477 IFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTVNRVTSSNGS 536

Query: 481 PSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFHYLYWASWY 515
            +K+GW   L   DLN + L+ FYW LA LS +NFF+YL+W+ WY
Sbjct: 537 GTKEGW---LGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWY 567