Bhi12M002052 (mRNA) Wax gourd (B227) v1

Overview


Name	Bhi12M002052
Type	mRNA
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Pyruvate dehydrogenase E1 component subunit alpha
Location	chr12: 73246923 .. 73250106 (-)
Sequence length	1948
RNA-Seq Expression	Bhi12M002052
Synteny	Bhi12M002052

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTAAATGACAAATATGTGTAGAAACTAGGAAGTAGTTGGTTTTCGTTGAGTGGCTTTCTTCTTTCTCCTACATTTCATCTTGTGCTCCAGTGAAAAACGAACACACCAAAATCCTCACACCTCAGAAAAGGCGGCGATTTCCAAGCTCCTCCTCGTTTCCTCCGCCCCTTTCTCCTTTCTCTTCCCCTTTTTATATATCTTCTCTTCTTTTTCCCTTTCATTTTCCCATCTATCTTTTCTCCTTTTTGCCATTTCCCCTTCTTCTTCTTCTTCTTCTTCTCCTCCTCCTCCTCCTCCTCCCACAATGTCCTTCTCTTCATCACTCAAAATCCTCCAACCCCTTCCTCTCAACTCCACCAGATCCAATGAAAAACCCCTTTTCTTTGATCCCTTTAGATCCTCCTCCAAGTTTCTTGGATCTCGATTCCGCATCCCTTCTCTATCTAAATCCAATACTCGCTCTCGATCTTCCCCTGCCGTTGCCGTTTCCGATGTTGTCAAGGAGAAGAAGCTCAAACCCAGTTCCAATTTGGTACTGTTTCTTCCATTTCTAGATTCTGAATTTGTTGCTTTGATTGTTTTTGTTATTGGGTTTTTAATGAATGTCCCGGAAATCATGGGGGAATTGGGTAGTGTAGCATGAAATTTCACTTTGTATGCCCGCATGGAATCATCTAGCTTTAACGATTTTGCTTTTGTTGGTTTTTGATTTGTTGATTTTGGTCTCCCTGTTGTTTTCGTTTGGAAATTTAGATGGTTTGAGATTTTACCATTATGAAATTGGAGGTTATTGTTTGTTCTTTTGCCCAACTAAGAATTAGCTTCGTTGAATTATTGGATTCGTGCAAAATTTTACTCTACTGGAAGCTTCTGTATGCATTGCTTTGCTACATGAACCATTTTGGTTTAAAAGTTCTTCCCCTTTTTTGGTTCCCTTTATTGTCTCCACACAGCTGATTACAAAAGAAGAGGGTTTGGTACTGTATGAGGACATGGTATTAGGCAGAGAGTTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCAACCGGGTTCATCAAGCTTCTTACAAAGAGGGATACTGTAGTGAGCACTTATCGTGATCACGTTCATGCTCTCAGCAAGGGAGTTCCATCCCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGTTGCTGCCGTGGTCAAGGTGGCTCGATGCATATGTTCTCGAAAGAACATAATCTGATAGGTGGCTTCGCCTTCATTGGGGAAGGCATACCAGTGGCCACTGGTGCTGCATTTACATCTAAATACAAGAGAGAGGTTTTAAAAGAGGCAGACTGTGAAGACGTCACTCTGGCGTTTTTCGGGGATGGAACTTGCAACAATGGCCAGTTCTTTGAGTGTTTGAACATGGCTGCTTTGTGGAAATTGCCAATTGTATTTGTCGTGGAGAATAATCTTTGGGCAATTGGAATGTCACATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAGAAGGGTCCTGCATTTGGAATGCCTGGCGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGGGAGGTTGCAAAAGAGGCAATTGGAAGAGCTAGGAGAGGAGAAGGACCAACTCTCGTGGAATGTGAAACCTATAGGTTTAGAGGACACTCACTGGCTGATCCAGATGAACTCCGTGACCCTGGTTAGTGCTCTTAAATTTATACCTACATTTGGTTCTGTCATTTCTAAATATGCATTTGCTTGGCCTTGTTGACATTTATTATTACGGCTAGCTAAAGATCCTTAATTCATTTCTCATAAATCATGATAGCTAATCTCTTAATCCGTACTCCATCATAGCATTTAAGGCTAAATCATTCAGGGTTGTAATGAGCTGCACCGCTATAAATTCACTCATTAGTTAACTTGAAAAAATTTCTCATTTGTTCATCATTTTTTACTGAACAAAGAGGGAAATAGGTTTTGTAGTTGATTTTGCCAGTGTTTAATCCAGAGTACACTGTCTGAGGCCAGATTGCATTGTTCATCTTCAAATGTCAGTCTACTGAATACAATTAATGGACTCATCTTGCAATGGTTACTATATAGATTCTAAGTGAACCAAACATCGTGGAAGTGGAAGTGTGGAAGTGACAGCTGATTCTTTCTTTTCTTCAAGTGGACATTGTAAAAGGAAGAAACCAGCCAAGCATGAAGGACATAACTACATTATTTGACTTTTACATGATTGCTCACAAGGAAAAACACTTGGAGCTCATGTGATATATATTTAGCAAATTAACATAATAATATGTAGATTTTTAATGTTCATCAGTTAAATGAGGTGTCTTTAGAGTTCATGGAGAAATTTGATTAGATGATAGAACTCAATGTCCCCATCAGGTTCTTCATTGTACTTTGCTTACTTACAGACTCAAATGTAGTAGTTTCTTATCAAAAAGCATAATAACTCTGGAAAGTACAAAGGATTAAGCATTCCTGTTTTAACCGTGGCAGATGAGAAGGCCCGTTATGCTGCTAGAGATCCCATTGTAGCTTTGAAGAAGTACTTGCTGGAGAATAATCTAGCCAGTGAACAAGAGCTAAAGGGAATAAAAGATAAGATAGTTGAGGTGGTTGAGGATGCAGTTAGTTTTGCAGATGAAAGTCCCCTTCCAGCCAGAAGCCAGTTATTAGAGAATGTTTTTGCTGATCCTAAAGGCTTTGGAATTGGGCCTGATGGAAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGCACTGCACATGTCTAAACTTCACAAAGTTCGTTATCTATCAAAGTCTTTATTTATTTTAATTGGTTTTCTTCACTGTTTTCTGTTGGGTTATTTCTGATTATTTGTAATTGAAGGTTAAATTAGCTTTTTCTGAAGGAACCAGGAATTTCTTTCCTTTCTTCAGGTCGTCCTTTAGTTTTTCCCAATTTTGATTTAGTGTATGATACAAAACGCTCCTCTACTAATCTAGTTATGTGGCAGAAGGAAAGCATTGTAATGCTTTACATATTACGTTTTAATATTTATGATCGATGAACAGTTTAAAGCCTATTTTATTCTTGAACTTTTATACTTTTATGTTTCGTATTCTATTTTTATTCTATTTTGGTCTC

mRNA sequence

GTTAAATGACAAATATGTGTAGAAACTAGGAAGTAGTTGGTTTTCGTTGAGTGGCTTTCTTCTTTCTCCTACATTTCATCTTGTGCTCCAGTGAAAAACGAACACACCAAAATCCTCACACCTCAGAAAAGGCGGCGATTTCCAAGCTCCTCCTCGTTTCCTCCGCCCCTTTCTCCTTTCTCTTCCCCTTTTTATATATCTTCTCTTCTTTTTCCCTTTCATTTTCCCATCTATCTTTTCTCCTTTTTGCCATTTCCCCTTCTTCTTCTTCTTCTTCTTCTCCTCCTCCTCCTCCTCCTCCCACAATGTCCTTCTCTTCATCACTCAAAATCCTCCAACCCCTTCCTCTCAACTCCACCAGATCCAATGAAAAACCCCTTTTCTTTGATCCCTTTAGATCCTCCTCCAAGTTTCTTGGATCTCGATTCCGCATCCCTTCTCTATCTAAATCCAATACTCGCTCTCGATCTTCCCCTGCCGTTGCCGTTTCCGATGTTGTCAAGGAGAAGAAGCTCAAACCCAGTTCCAATTTGCTGATTACAAAAGAAGAGGGTTTGGTACTGTATGAGGACATGGTATTAGGCAGAGAGTTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCAACCGGGTTCATCAAGCTTCTTACAAAGAGGGATACTGTAGTGAGCACTTATCGTGATCACGTTCATGCTCTCAGCAAGGGAGTTCCATCCCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGTTGCTGCCGTGGTCAAGGTGGCTCGATGCATATGTTCTCGAAAGAACATAATCTGATAGGTGGCTTCGCCTTCATTGGGGAAGGCATACCAGTGGCCACTGGTGCTGCATTTACATCTAAATACAAGAGAGAGGTTTTAAAAGAGGCAGACTGTGAAGACGTCACTCTGGCGTTTTTCGGGGATGGAACTTGCAACAATGGCCAGTTCTTTGAGTGTTTGAACATGGCTGCTTTGTGGAAATTGCCAATTGTATTTGTCGTGGAGAATAATCTTTGGGCAATTGGAATGTCACATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAGAAGGGTCCTGCATTTGGAATGCCTGGCGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGGGAGGTTGCAAAAGAGGCAATTGGAAGAGCTAGGAGAGGAGAAGGACCAACTCTCGTGGAATGTGAAACCTATAGGTTTAGAGGACACTCACTGGCTGATCCAGATGAACTCCGTGACCCTGATGAGAAGGCCCGTTATGCTGCTAGAGATCCCATTGTAGCTTTGAAGAAGTACTTGCTGGAGAATAATCTAGCCAGTGAACAAGAGCTAAAGGGAATAAAAGATAAGATAGTTGAGGTGGTTGAGGATGCAGTTAGTTTTGCAGATGAAAGTCCCCTTCCAGCCAGAAGCCAGTTATTAGAGAATGTTTTTGCTGATCCTAAAGGCTTTGGAATTGGGCCTGATGGAAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGCACTGCACATGTCTAAACTTCACAAAGTTCGTTATCTATCAAAGTCTTTATTTATTTTAATTGGTTTTCTTCACTGTTTTCTGTTGGGTTATTTCTGATTATTTGTAATTGAAGGTTAAATTAGCTTTTTCTGAAGGAACCAGGAATTTCTTTCCTTTCTTCAGGTCGTCCTTTAGTTTTTCCCAATTTTGATTTAGTGTATGATACAAAACGCTCCTCTACTAATCTAGTTATGTGGCAGAAGGAAAGCATTGTAATGCTTTACATATTACGTTTTAATATTTATGATCGATGAACAGTTTAAAGCCTATTTTATTCTTGAACTTTTATACTTTTATGTTTCGTATTCTATTTTTATTCTATTTTGGTCTC

Coding sequence (CDS)

ATGTCCTTCTCTTCATCACTCAAAATCCTCCAACCCCTTCCTCTCAACTCCACCAGATCCAATGAAAAACCCCTTTTCTTTGATCCCTTTAGATCCTCCTCCAAGTTTCTTGGATCTCGATTCCGCATCCCTTCTCTATCTAAATCCAATACTCGCTCTCGATCTTCCCCTGCCGTTGCCGTTTCCGATGTTGTCAAGGAGAAGAAGCTCAAACCCAGTTCCAATTTGCTGATTACAAAAGAAGAGGGTTTGGTACTGTATGAGGACATGGTATTAGGCAGAGAGTTTGAGGATATGTGTGCACAGATGTATTACAGAGGCAAAATGTTTGGTTTTGTTCACCTTTACAATGGCCAAGAAGCTGTTTCAACCGGGTTCATCAAGCTTCTTACAAAGAGGGATACTGTAGTGAGCACTTATCGTGATCACGTTCATGCTCTCAGCAAGGGAGTTCCATCCCGTGAGGTCATGAGTGAGCTTTTTGGAAAGACCACTGGTTGCTGCCGTGGTCAAGGTGGCTCGATGCATATGTTCTCGAAAGAACATAATCTGATAGGTGGCTTCGCCTTCATTGGGGAAGGCATACCAGTGGCCACTGGTGCTGCATTTACATCTAAATACAAGAGAGAGGTTTTAAAAGAGGCAGACTGTGAAGACGTCACTCTGGCGTTTTTCGGGGATGGAACTTGCAACAATGGCCAGTTCTTTGAGTGTTTGAACATGGCTGCTTTGTGGAAATTGCCAATTGTATTTGTCGTGGAGAATAATCTTTGGGCAATTGGAATGTCACATTTGAGGGCTACTTCAGATCCTGAGATTTGGAAGAAGGGTCCTGCATTTGGAATGCCTGGCGTTCATGTTGATGGTATGGATGTTTTGAAGGTGAGGGAGGTTGCAAAAGAGGCAATTGGAAGAGCTAGGAGAGGAGAAGGACCAACTCTCGTGGAATGTGAAACCTATAGGTTTAGAGGACACTCACTGGCTGATCCAGATGAACTCCGTGACCCTGATGAGAAGGCCCGTTATGCTGCTAGAGATCCCATTGTAGCTTTGAAGAAGTACTTGCTGGAGAATAATCTAGCCAGTGAACAAGAGCTAAAGGGAATAAAAGATAAGATAGTTGAGGTGGTTGAGGATGCAGTTAGTTTTGCAGATGAAAGTCCCCTTCCAGCCAGAAGCCAGTTATTAGAGAATGTTTTTGCTGATCCTAAAGGCTTTGGAATTGGGCCTGATGGAAAGTACAGATGTGAGGATCCCAAGTTCACAGAAGGCACTGCACATGTCTAA

Protein sequence

MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVAVSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNLASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPKFTEGTAHV

Homology

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BLAST of Bhi12M002052 vs. TAIR 10
Match: AT1G01090.1 (pyruvate dehydrogenase E1 alpha )

HSP 1 Score: 673.3 bits (1736), Expect = 1.3e-193
Identity = 338/425 (79.53%), Postives = 370/425 (87.06%), Query Frame = 0


Query: 8   KILQPLPLNSTRSNEKPLFFDPFRSS--SKFLGSRFRIPSLSKSN--TRSRSSPAVAVSD 67
           K+   +PL+ +  N   L   P R +  S FLGS  R  SL + N    +R SP V+V +
Sbjct: 9   KLTATVPLHGSHENRLLL---PIRLAPPSSFLGST-RSLSLRRLNHSNATRRSPVVSVQE 68

Query: 68  VVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQEAVS 127
           VVKEK+   +++LLITKEEGL LYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQEAVS
Sbjct: 69  VVKEKQSTNNTSLLITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVS 128

Query: 128 TGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHN 187
           TGFIKLLTK D+VVSTYRDHVHALSKGV +R VMSELFGK TGCCRGQGGSMHMFSKEHN
Sbjct: 129 TGFIKLLTKSDSVVSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHN 188

Query: 188 LIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAA 247
           ++GGFAFIGEGIPVATGAAF+SKY+REVLK+ DC+DVT+AFFGDGTCNNGQFFECLNMAA
Sbjct: 189 MLGGFAFIGEGIPVATGAAFSSKYRREVLKQ-DCDDVTVAFFGDGTCNNGQFFECLNMAA 248

Query: 248 LWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAI 307
           L+KLPI+FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEA+
Sbjct: 249 LYKLPIIFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAV 308

Query: 308 GRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNLASE 367
            RARRGEGPTLVECETYRFRGHSLADPDELRD  EKA+YAARDPI ALKKYL+EN LA E
Sbjct: 309 TRARRGEGPTLVECETYRFRGHSLADPDELRDAAEKAKYAARDPIAALKKYLIENKLAKE 368

Query: 368 QELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPKFTE 427
            ELK I+ KI E+VE+AV FAD SP P RSQLLENVFADPKGFGIGPDG+YRCEDPKFTE
Sbjct: 369 AELKSIEKKIDELVEEAVEFADASPQPGRSQLLENVFADPKGFGIGPDGRYRCEDPKFTE 428

Query: 428 GTAHV 429
           GTA V
Sbjct: 429 GTAQV 428

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BLAST of Bhi12M002052 vs. TAIR 10
Match: AT1G59900.1 (pyruvate dehydrogenase complex E1 alpha subunit )

HSP 1 Score: 261.5 bits (667), Expect = 1.2e-69
Identity = 143/348 (41.09%), Postives = 205/348 (58.91%), Query Frame = 0


Query: 72  PSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMF-GFVHLYNGQEAVSTGFIKLL 131
           PS ++  + +E L  +  M L R  E + A   Y+ K+  GF HLY+GQEAV+ G    +
Sbjct: 48  PSRSVESSSQELLDFFRTMALMRRME-IAADSLYKAKLIRGFCHLYDGQEAVAIGMEAAI 107

Query: 132 TKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAF 191
           TK+D +++ YRDH   L +G    EV SEL G+  GC +G+GGSMH + KE +  GG   
Sbjct: 108 TKKDAIITAYRDHCIFLGRGGSLHEVFSELMGRQAGCSKGKGGSMHFYKKESSFYGGHGI 167

Query: 192 IGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIV 251
           +G  +P+  G AF  KY +E       E VT A +GDG  N GQ FE LN++ALW LP +
Sbjct: 168 VGAQVPLGCGIAFAQKYNKE-------EAVTFALYGDGAANQGQLFEALNISALWDLPAI 227

Query: 252 FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGE 311
            V ENN + +G +  RA   P  +K+G    +PG+ VDGMD   V++  K A   A   +
Sbjct: 228 LVCENNHYGMGTAEWRAAKSPSYYKRGDY--VPGLKVDGMDAFAVKQACKFAKQHALE-K 287

Query: 312 GPTLVECETYRFRGHSLADP-DELRDPDE-KARYAARDPIVALKKYLLENNLASEQELKG 371
           GP ++E +TYR+ GHS++DP    R  DE       RDPI  +KK +L ++LA+E+ELK 
Sbjct: 288 GPIILEMDTYRYHGHSMSDPGSTYRTRDEISGVRQERDPIERIKKLVLSHDLATEKELKD 347

Query: 372 IKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFG---IGPDGK 414
           ++ +I + V+DA++ A + P+P  S+L  NV+   KGFG    GPD K
Sbjct: 348 MEKEIRKEVDDAIAKAKDCPMPEPSELFTNVYV--KGFGTESFGPDRK 382

[Close]

BLAST of Bhi12M002052 vs. TAIR 10
Match: AT1G24180.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 5.4e-67
Identity = 144/377 (38.20%), Postives = 215/377 (57.03%), Query Frame = 0


Query: 43  IPSLSKSNTRSRSSPAVAVSDVVKEKKL--KPSSNLLITKEEGLVLYEDMVLGREFEDMC 102
           +PS  + +  + SSP    + V     L   PS ++  + EE L  + DM   R  E + 
Sbjct: 21  LPSSIRRHVSTDSSPITIETAVPFTSHLCESPSRSVETSSEEILAFFRDMARMRRME-IA 80

Query: 103 AQMYYRGKMF-GFVHLYNGQEAVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSE 162
           A   Y+ K+  GF HLY+GQEA++ G    +TK+D ++++YRDH   + +G    +  SE
Sbjct: 81  ADSLYKAKLIRGFCHLYDGQEALAVGMEAAITKKDAIITSYRDHCTFIGRGGKLVDAFSE 140

Query: 163 LFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCED 222
           L G+ TGC  G+GGSMH + K+ +  GG   +G  IP+  G AF  KY ++       E 
Sbjct: 141 LMGRKTGCSHGKGGSMHFYKKDASFYGGHGIVGAQIPLGCGLAFAQKYNKD-------EA 200

Query: 223 VTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPA 282
           VT A +GDG  N GQ FE LN++ALW LP + V ENN + +G +  R+   P  +K+G  
Sbjct: 201 VTFALYGDGAANQGQLFEALNISALWDLPAILVCENNHYGMGTATWRSAKSPAYFKRGDY 260

Query: 283 FGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVECETYRFRGHSLADP-DELRDPDE 342
             +PG+ VDGMD L V++  K A   A +  GP ++E +TYR+ GHS++DP    R  DE
Sbjct: 261 --VPGLKVDGMDALAVKQACKFAKEHALK-NGPIILEMDTYRYHGHSMSDPGSTYRTRDE 320

Query: 343 -KARYAARDPIVALKKYLLENNLASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLE 402
                  RDPI  ++K LL +++A+E+ELK ++ +I + V+DAV+ A ESP+P  S+L  
Sbjct: 321 ISGVRQVRDPIERVRKLLLTHDIATEKELKDMEKEIRKEVDDAVAQAKESPIPDASELFT 380

Query: 403 NVFADPKGF-GIGPDGK 414
           N++    G    G D K
Sbjct: 381 NMYVKDCGVESFGADRK 386

[Close]

BLAST of Bhi12M002052 vs. TAIR 10
Match: AT5G09300.1 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 124.8 bits (312), Expect = 1.7e-28
Identity = 85/327 (25.99%), Postives = 157/327 (48.01%), Query Frame = 0


Query: 78  ITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVV 137
           +++E  + +Y DMV  +  +++  +   +G++  F     G+EA++      LT +D + 
Sbjct: 125 VSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRL-SFYATAIGEEAINIASAAALTPQDVIF 184

Query: 138 STYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPV 197
             YR+    L +G   +E  ++ FG  +   +G+   +H  S + N     A I   +P 
Sbjct: 185 PQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPN 244

Query: 198 ATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 257
           A GAA++ K  ++       +   + +FGDG  + G F   LN+AA+ + P++F+  NN 
Sbjct: 245 AVGAAYSLKMDKK-------DACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNG 304

Query: 258 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 317
           WAI            +  KG A+G+  + VDG D L +      A   A R + P L+E 
Sbjct: 305 WAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEA 364

Query: 318 ETYRFRGHSLADPD-ELRDPDEKARY-AARDPIVALKKYLLENNLASEQELKGIKDKIVE 377
            TYR   HS +D     R   E   +  AR+P+   + ++  N   S++    ++ +I +
Sbjct: 365 LTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKK 424

Query: 378 VVEDAVSFADESPLPARSQLLENVFAD 403
            + +A+  A+++  P     L+N+F+D
Sbjct: 425 EMLEALRVAEKTEKPN----LQNMFSD 439

[Close]

BLAST of Bhi12M002052 vs. TAIR 10
Match: AT5G09300.2 (Thiamin diphosphate-binding fold (THDP-binding) superfamily protein )

HSP 1 Score: 124.8 bits (312), Expect = 1.7e-28
Identity = 85/327 (25.99%), Postives = 157/327 (48.01%), Query Frame = 0


Query: 78  ITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTKRDTVV 137
           +++E  + +Y DMV  +  +++  +   +G++  F     G+EA++      LT +D + 
Sbjct: 54  VSEEVAVKIYSDMVTLQIMDNIFYEAQRQGRL-SFYATAIGEEAINIASAAALTPQDVIF 113

Query: 138 STYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIGEGIPV 197
             YR+    L +G   +E  ++ FG  +   +G+   +H  S + N     A I   +P 
Sbjct: 114 PQYREPGVLLWRGFTLQEFANQCFGNKSDYGKGRQMPVHYGSNKLNYFTVSATIATQLPN 173

Query: 198 ATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFVVENNL 257
           A GAA++ K  ++       +   + +FGDG  + G F   LN+AA+ + P++F+  NN 
Sbjct: 174 AVGAAYSLKMDKK-------DACAVTYFGDGGTSEGDFHAALNIAAVMEAPVLFICRNNG 233

Query: 258 WAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGPTLVEC 317
           WAI            +  KG A+G+  + VDG D L +      A   A R + P L+E 
Sbjct: 234 WAISTPTSDQFRSDGVVVKGRAYGIRSIRVDGNDALAMYSAVHTAREMAIREQRPILIEA 293

Query: 318 ETYRFRGHSLADPD-ELRDPDEKARY-AARDPIVALKKYLLENNLASEQELKGIKDKIVE 377
            TYR   HS +D     R   E   +  AR+P+   + ++  N   S++    ++ +I +
Sbjct: 294 LTYRVGHHSTSDDSTRYRSAGEIEWWNKARNPLSRFRTWIESNGWWSDKTESDLRSRIKK 353

Query: 378 VVEDAVSFADESPLPARSQLLENVFAD 403
            + +A+  A+++  P     L+N+F+D
Sbjct: 354 EMLEALRVAEKTEKPN----LQNMFSD 368

[Close]

BLAST of Bhi12M002052 vs. ExPASy Swiss-Prot
Match: O24457 (Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PDH-E1 ALPHA PE=2 SV=1)

HSP 1 Score: 673.3 bits (1736), Expect = 1.8e-192
Identity = 338/425 (79.53%), Postives = 370/425 (87.06%), Query Frame = 0


Query: 8   KILQPLPLNSTRSNEKPLFFDPFRSS--SKFLGSRFRIPSLSKSN--TRSRSSPAVAVSD 67
           K+   +PL+ +  N   L   P R +  S FLGS  R  SL + N    +R SP V+V +
Sbjct: 9   KLTATVPLHGSHENRLLL---PIRLAPPSSFLGST-RSLSLRRLNHSNATRRSPVVSVQE 68

Query: 68  VVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQEAVS 127
           VVKEK+   +++LLITKEEGL LYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQEAVS
Sbjct: 69  VVKEKQSTNNTSLLITKEEGLELYEDMILGRSFEDMCAQMYYRGKMFGFVHLYNGQEAVS 128

Query: 128 TGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHN 187
           TGFIKLLTK D+VVSTYRDHVHALSKGV +R VMSELFGK TGCCRGQGGSMHMFSKEHN
Sbjct: 129 TGFIKLLTKSDSVVSTYRDHVHALSKGVSARAVMSELFGKVTGCCRGQGGSMHMFSKEHN 188

Query: 188 LIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAA 247
           ++GGFAFIGEGIPVATGAAF+SKY+REVLK+ DC+DVT+AFFGDGTCNNGQFFECLNMAA
Sbjct: 189 MLGGFAFIGEGIPVATGAAFSSKYRREVLKQ-DCDDVTVAFFGDGTCNNGQFFECLNMAA 248

Query: 248 LWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAI 307
           L+KLPI+FVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEA+
Sbjct: 249 LYKLPIIFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAV 308

Query: 308 GRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNLASE 367
            RARRGEGPTLVECETYRFRGHSLADPDELRD  EKA+YAARDPI ALKKYL+EN LA E
Sbjct: 309 TRARRGEGPTLVECETYRFRGHSLADPDELRDAAEKAKYAARDPIAALKKYLIENKLAKE 368

Query: 368 QELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPKFTE 427
            ELK I+ KI E+VE+AV FAD SP P RSQLLENVFADPKGFGIGPDG+YRCEDPKFTE
Sbjct: 369 AELKSIEKKIDELVEEAVEFADASPQPGRSQLLENVFADPKGFGIGPDGRYRCEDPKFTE 428

Query: 428 GTAHV 429
           GTA V
Sbjct: 429 GTAQV 428

[Close]

BLAST of Bhi12M002052 vs. ExPASy Swiss-Prot
Match: Q7XTJ3 (Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0119400 PE=2 SV=2)

HSP 1 Score: 636.0 bits (1639), Expect = 3.2e-181
Identity = 323/431 (74.94%), Postives = 358/431 (83.06%), Query Frame = 0


Query: 2   SFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVAV 61
           SF+++ K L P+   S    + PL   P  +S+     R       K   R R++ AV+ 
Sbjct: 6   SFTAAAKFLAPVSARSAGDYKPPL---PLPASASLRPGR-------KPAPRLRTALAVS- 65

Query: 62  SDVVKEKKLKP--SSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQ 121
           SDV+   K  P  +++  +T+EE L LYEDMVLGR FEDMCAQMYYRGKMFGFVHLYNGQ
Sbjct: 66  SDVLPGNKAAPAAAAHSAVTREEALELYEDMVLGRIFEDMCAQMYYRGKMFGFVHLYNGQ 125

Query: 122 EAVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFS 181
           EAVSTGFIKLL + D VVSTYRDHVHALSKGVP+R VM+ELFGK TGCCRGQGGSMHMFS
Sbjct: 126 EAVSTGFIKLLNQADCVVSTYRDHVHALSKGVPARSVMAELFGKATGCCRGQGGSMHMFS 185

Query: 182 KEHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEA--DCEDVTLAFFGDGTCNNGQFFE 241
           + HNL+GGFAFIGEGIPVATGAAF +KY+ EVLK++  D  DVTLAFFGDGTCNNGQFFE
Sbjct: 186 EPHNLLGGFAFIGEGIPVATGAAFAAKYRHEVLKQSSPDGLDVTLAFFGDGTCNNGQFFE 245

Query: 242 CLNMAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVRE 301
           CLNMA LWKLPIVFVVENNLWAIGMSHLRATSDPEI+KKGPAFGMPGVHVDGMDVLKVRE
Sbjct: 246 CLNMAQLWKLPIVFVVENNLWAIGMSHLRATSDPEIYKKGPAFGMPGVHVDGMDVLKVRE 305

Query: 302 VAKEAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLE 361
           VAKEAI RARRGEGPTLVECETYRFRGHSLADPDELR PDEK+ YAARDPI ALKKY++E
Sbjct: 306 VAKEAIERARRGEGPTLVECETYRFRGHSLADPDELRRPDEKSHYAARDPITALKKYIIE 365

Query: 362 NNLASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCE 421
            NLA+E ELK I+ KI +VVE+AV FAD SPLP RSQLLENVF+DPKGFGIGPDGKYRCE
Sbjct: 366 QNLATESELKSIEKKIDDVVEEAVEFADASPLPPRSQLLENVFSDPKGFGIGPDGKYRCE 425

Query: 422 DPKFTEGTAHV 429
           DP FT+GTA V
Sbjct: 426 DPLFTQGTAQV 425

[Close]

BLAST of Bhi12M002052 vs. ExPASy Swiss-Prot
Match: Q1XDM0 (Pyruvate dehydrogenase E1 component subunit alpha OS=Pyropia yezoensis OX=2788 GN=pdhA PE=3 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 1.1e-128
Identity = 224/330 (67.88%), Postives = 261/330 (79.09%), Query Frame = 0


Query: 73  SSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGFIKLLTK 132
           S+ L + K   LVLYEDM+LGR FEDMCAQMYY+GKMFGFVHLYNGQEAVSTG IKLL  
Sbjct: 16  STGLNLNKSNLLVLYEDMLLGRNFEDMCAQMYYKGKMFGFVHLYNGQEAVSTGVIKLLNP 75

Query: 133 RDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIGGFAFIG 192
            D V STYRDHVHALSKGVPS+ VM+ELFGK TGC +G+GGSMH+FS  HN +GGFAFI 
Sbjct: 76  TDYVCSTYRDHVHALSKGVPSKNVMAELFGKETGCSKGRGGSMHIFSAPHNFLGGFAFIA 135

Query: 193 EGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWKLPIVFV 252
           EGIPVATGAAF S Y+++VLKE +   VT  FFGDGT NNGQFFECLNMA LWKLPI+FV
Sbjct: 136 EGIPVATGAAFQSIYRQQVLKETEDLRVTACFFGDGTTNNGQFFECLNMAVLWKLPIIFV 195

Query: 253 VENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRARRGEGP 312
           VENN WAIGM+H R++S PEI KK  AFG+PG+ VDGMDVL VR+ AK+A+ RAR+G+GP
Sbjct: 196 VENNQWAIGMAHHRSSSIPEIHKKAEAFGLPGIEVDGMDVLAVRQAAKQAVQRARQGDGP 255

Query: 313 TLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNLASEQELKGIKDK 372
           TL+E  TYRFRGHSLADPDELR   EK  + ARDPI  LKKY+L+N +A+  EL  I++ 
Sbjct: 256 TLIEALTYRFRGHSLADPDELRSRQEKEAWVARDPIKKLKKYILDNEIANIGELNEIQNA 315

Query: 373 IVEVVEDAVSFADESPLPARSQLLENVFAD 403
           +   +E AV FA  SP P  S+L   +FAD
Sbjct: 316 VKTELEQAVKFAISSPEPNMSELKRYLFAD 345

[Close]

BLAST of Bhi12M002052 vs. ExPASy Swiss-Prot
Match: P51267 (Pyruvate dehydrogenase E1 component subunit alpha OS=Porphyra purpurea OX=2787 GN=pdhA PE=3 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 4.1e-128
Identity = 225/336 (66.96%), Postives = 264/336 (78.57%), Query Frame = 0


Query: 67  EKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQEAVSTGF 126
           E  L   + + +TK + LVLYEDM+LGR FEDMCAQMYY+GKMFGFVHLYNGQEAVSTG 
Sbjct: 8   ELPLTNCNQINLTKHKLLVLYEDMLLGRNFEDMCAQMYYKGKMFGFVHLYNGQEAVSTGV 67

Query: 127 IKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNLIG 186
           IKLL  +D V STYRDHVHALSKGVPS+ VM+ELFGK TGC RG+GGSMH+FS  HN +G
Sbjct: 68  IKLLDSKDYVCSTYRDHVHALSKGVPSQNVMAELFGKETGCSRGRGGSMHIFSAPHNFLG 127

Query: 187 GFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAALWK 246
           GFAFI EGIPVATGAAF S Y+++VLKE     VT  FFGDGT NNGQFFECLNMA LWK
Sbjct: 128 GFAFIAEGIPVATGAAFQSIYRQQVLKEPGELRVTACFFGDGTTNNGQFFECLNMAVLWK 187

Query: 247 LPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIGRA 306
           LPI+FVVENN WAIGM+H R++S PEI KK  AFG+PG+ VDGMDVL VR+VA++A+ RA
Sbjct: 188 LPIIFVVENNQWAIGMAHHRSSSIPEIHKKAEAFGLPGIEVDGMDVLAVRQVAEKAVERA 247

Query: 307 RRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNLASEQEL 366
           R+G+GPTL+E  TYRFRGHSLADPDELR   EK  + ARDPI  LKK++L+N +AS  EL
Sbjct: 248 RQGQGPTLIEALTYRFRGHSLADPDELRSRQEKEAWVARDPIKKLKKHILDNQIASSDEL 307

Query: 367 KGIKDKIVEVVEDAVSFADESPLPARSQLLENVFAD 403
             I+  +   +E +V FA  SP P  S+L   +FAD
Sbjct: 308 NDIQSSVKIDLEQSVEFAMSSPEPNISELKRYLFAD 343

[Close]

BLAST of Bhi12M002052 vs. ExPASy Swiss-Prot
Match: Q4UKQ6 (Pyruvate dehydrogenase E1 component subunit alpha OS=Rickettsia felis (strain ATCC VR-1525 / URRWXCal2) OX=315456 GN=pdhA PE=3 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.6e-76
Identity = 136/336 (40.48%), Postives = 208/336 (61.90%), Query Frame = 0


Query: 65  VKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQEAVST 124
           +K KK KP      TKEE +  ++DM+L R FE+ C Q+Y  G++ GF HLY GQEAV +
Sbjct: 3   IKPKKYKP------TKEEYIKSFKDMLLLRRFEEKCGQLYGMGEIGGFCHLYIGQEAVIS 62

Query: 125 GFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSKEHNL 184
               +  K D+ +++YRDH H +  G   + V++EL G+ TGC +G+GGSMH+F   +  
Sbjct: 63  AVDTVKQKGDSTITSYRDHAHIILAGTEPKYVLAELMGRATGCSKGKGGSMHLFDIPNKF 122

Query: 185 IGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLNMAAL 244
            GG   +G  +P+ TG AF  KY           ++   F GDG  N GQ +E  NMAAL
Sbjct: 123 YGGHGIVGAQVPIGTGLAFAEKYN-------GTNNICFTFLGDGAVNQGQVYEAFNMAAL 182

Query: 245 WKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAKEAIG 304
           W LP+V+++ENN +++G S  R+T   +++KKG +FG+ G  +DGMD  ++   AK+A  
Sbjct: 183 WGLPVVYIIENNEYSMGTSVARSTFMRDLYKKGESFGIKGFQLDGMDFEEMYNGAKQAAE 242

Query: 305 RARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNLASEQ 364
             R    P ++E +TYR+RGHS++DP + R  +E  +Y  RDP+V ++K +L+N  A+E 
Sbjct: 243 YVRENSFPLILEVKTYRYRGHSMSDPAKYRSKEEVEQYKERDPLVIIRKTILDNKYATEA 302

Query: 365 ELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVF 401
           +LK I+  + E+V++AV F++ SPLP   +L   V+
Sbjct: 303 DLKEIEQSVKEIVKEAVKFSENSPLPDEGELYTEVY 325

[Close]

BLAST of Bhi12M002052 vs. NCBI nr
Match: XP_038875269.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Benincasa hispida])

HSP 1 Score: 865.9 bits (2236), Expect = 1.4e-247
Identity = 428/428 (100.00%), Postives = 428/428 (100.00%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Bhi12M002052 vs. NCBI nr
Match: XP_008459830.1 (PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis melo] >KAA0039805.1 pyruvate dehydrogenase E1 component subunit alpha-3 [Cucumis melo var. makuwa] >TYK24692.1 pyruvate dehydrogenase E1 component subunit alpha-3 [Cucumis melo var. makuwa])

HSP 1 Score: 842.8 bits (2176), Expect = 1.3e-240
Identity = 412/428 (96.26%), Postives = 420/428 (98.13%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFSSSLKILQPLPLNSTRSN+KPL FDPFRS+SKFLGSRFR+PSLSKSNTR RSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDM+LGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPI ALKKYLLEN L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELK IKDKIVEVVEDAV FADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Bhi12M002052 vs. NCBI nr
Match: XP_004140530.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis sativus] >KGN46471.1 hypothetical protein Csa_005756 [Cucumis sativus])

HSP 1 Score: 840.1 bits (2169), Expect = 8.4e-240
Identity = 411/428 (96.03%), Postives = 420/428 (98.13%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFSSSLKILQPLPLNSTRSN+KPL FDPFRS+SKFLGSRFR+PSLSKSNTR RSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDM+LGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPI ALKKY+LEN L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELK IKDKIVEVVE+AV FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Bhi12M002052 vs. NCBI nr
Match: KAG6575304.1 (Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 808.9 bits (2088), Expect = 2.1e-230
Identity = 398/428 (92.99%), Postives = 409/428 (95.56%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MS S+S KILQPL LNS RSN+KPLFFDPFRS+S FLGSRFR PS+SKS TRSRSSP VA
Sbjct: 1   MSLSASPKILQPLLLNSPRSNQKPLFFDPFRSTSNFLGSRFRFPSISKSTTRSRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLVLYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVLYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPI ALKKYL+E NL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ASEQELK I+ +I EVVE+AV FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIDEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Bhi12M002052 vs. NCBI nr
Match: XP_022992260.1 (pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita maxima])

HSP 1 Score: 807.7 bits (2085), Expect = 4.6e-230
Identity = 397/428 (92.76%), Postives = 408/428 (95.33%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFS+S KILQPL LNS RSNEKPLFFDPFRS+S FLGSRFR PS+SKS TR RSSP VA
Sbjct: 1   MSFSASPKILQPLLLNSPRSNEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLV+YEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVVYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEGDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPI ALKKYL+E NL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ASEQELK I+ +I EVVE+AV FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIGEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Bhi12M002052 vs. ExPASy TrEMBL
Match: A0A5A7TEC1 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002450 PE=4 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 6.3e-241
Identity = 412/428 (96.26%), Postives = 420/428 (98.13%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFSSSLKILQPLPLNSTRSN+KPL FDPFRS+SKFLGSRFR+PSLSKSNTR RSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDM+LGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPI ALKKYLLEN L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELK IKDKIVEVVEDAV FADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Bhi12M002052 vs. ExPASy TrEMBL
Match: A0A1S3CB66 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo OX=3656 GN=LOC103498837 PE=4 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 6.3e-241
Identity = 412/428 (96.26%), Postives = 420/428 (98.13%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFSSSLKILQPLPLNSTRSN+KPL FDPFRS+SKFLGSRFR+PSLSKSNTR RSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPTVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDM+LGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHA+SKGVPSR+VMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHAISKGVPSRQVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKETDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPI ALKKYLLEN L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYLLENKL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELK IKDKIVEVVEDAV FADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEDAVQFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Bhi12M002052 vs. ExPASy TrEMBL
Match: A0A0A0KFF3 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus OX=3659 GN=Csa_6G095870 PE=4 SV=1)

HSP 1 Score: 840.1 bits (2169), Expect = 4.1e-240
Identity = 411/428 (96.03%), Postives = 420/428 (98.13%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFSSSLKILQPLPLNSTRSN+KPL FDPFRS+SKFLGSRFR+PSLSKSNTR RSSP VA
Sbjct: 1   MSFSSSLKILQPLPLNSTRSNDKPLLFDPFRSTSKFLGSRFRLPSLSKSNTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLKPSSNLLITKEEGLVLYEDM+LGREFEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMILGREFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLT+RDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTQRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADC+DVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCQDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPI ALKKY+LEN L
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIAALKKYMLENKL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           A+EQELK IKDKIVEVVE+AV FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ANEQELKAIKDKIVEVVEEAVQFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Bhi12M002052 vs. ExPASy TrEMBL
Match: A0A6J1JV75 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita maxima OX=3661 GN=LOC111488634 PE=4 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 2.2e-230
Identity = 397/428 (92.76%), Postives = 408/428 (95.33%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MSFS+S KILQPL LNS RSNEKPLFFDPFRS+S FLGSRFR PS+SKS TR RSSP VA
Sbjct: 1   MSFSASPKILQPLLLNSPRSNEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLV+YEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVVYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKE DCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEGDCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPI ALKKYL+E NL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ASEQELK I+ +I EVVE+AV FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIGEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

[Close]

BLAST of Bhi12M002052 vs. ExPASy TrEMBL
Match: A0A6J1EPB1 (Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 GN=LOC111436336 PE=4 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 8.5e-230
Identity = 397/428 (92.76%), Postives = 407/428 (95.09%), Query Frame = 0


Query: 1   MSFSSSLKILQPLPLNSTRSNEKPLFFDPFRSSSKFLGSRFRIPSLSKSNTRSRSSPAVA 60
           MS S+S KILQPL LNS RS EKPLFFDPFRS+S FLGSRFR PS+SKS TR RSSP VA
Sbjct: 1   MSLSASPKILQPLLLNSPRSTEKPLFFDPFRSTSNFLGSRFRFPSISKSTTRCRSSPVVA 60

Query: 61  VSDVVKEKKLKPSSNLLITKEEGLVLYEDMVLGREFEDMCAQMYYRGKMFGFVHLYNGQE 120
           VSDVVKEKKLK  SNLLITKEEGLVLYEDM+LGR FEDMCAQMYYRGKMFGFVHLYNGQE
Sbjct: 61  VSDVVKEKKLKSGSNLLITKEEGLVLYEDMILGRVFEDMCAQMYYRGKMFGFVHLYNGQE 120

Query: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPSREVMSELFGKTTGCCRGQGGSMHMFSK 180
           AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVP+REVMSELFGKTTGCCRGQGGSMHMFSK
Sbjct: 121 AVSTGFIKLLTKRDTVVSTYRDHVHALSKGVPAREVMSELFGKTTGCCRGQGGSMHMFSK 180

Query: 181 EHNLIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240
           EHN+IGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN
Sbjct: 181 EHNVIGGFAFIGEGIPVATGAAFTSKYKREVLKEADCEDVTLAFFGDGTCNNGQFFECLN 240

Query: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300
           MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK
Sbjct: 241 MAALWKLPIVFVVENNLWAIGMSHLRATSDPEIWKKGPAFGMPGVHVDGMDVLKVREVAK 300

Query: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPDEKARYAARDPIVALKKYLLENNL 360
           EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDP EKARYAARDPI ALKKYL+E NL
Sbjct: 301 EAIGRARRGEGPTLVECETYRFRGHSLADPDELRDPAEKARYAARDPIAALKKYLVEKNL 360

Query: 361 ASEQELKGIKDKIVEVVEDAVSFADESPLPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420
           ASEQELK I+ +I EVVE+AV FADESP PARSQLLENVFADPKGFGIGPDGKYRCEDPK
Sbjct: 361 ASEQELKAIEKRIDEVVEEAVEFADESPHPARSQLLENVFADPKGFGIGPDGKYRCEDPK 420

Query: 421 FTEGTAHV 429
           FTEGTAHV
Sbjct: 421 FTEGTAHV 428

The following BLAST results are available for this feature:

BLAST of Bhi12M002052 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G01090.1	1.3e-193	79.53	pyruvate dehydrogenase E1 alpha	[more]
AT1G59900.1	1.2e-69	41.09	pyruvate dehydrogenase complex E1 alpha subunit	[more]
AT1G24180.1	5.4e-67	38.20	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G09300.1	1.7e-28	25.99	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]
AT5G09300.2	1.7e-28	25.99	Thiamin diphosphate-binding fold (THDP-binding) superfamily protein	[more]

BLAST of Bhi12M002052 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O24457	1.8e-192	79.53	Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Arabidopsi...	[more]
Q7XTJ3	3.2e-181	74.94	Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic OS=Oryza sati...	[more]
Q1XDM0	1.1e-128	67.88	Pyruvate dehydrogenase E1 component subunit alpha OS=Pyropia yezoensis OX=2788 G...	[more]
P51267	4.1e-128	66.96	Pyruvate dehydrogenase E1 component subunit alpha OS=Porphyra purpurea OX=2787 G...	[more]
Q4UKQ6	1.6e-76	40.48	Pyruvate dehydrogenase E1 component subunit alpha OS=Rickettsia felis (strain AT...	[more]

BLAST of Bhi12M002052 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875269.1	1.4e-247	100.00	pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Benincasa hi...	[more]
XP_008459830.1	1.3e-240	96.26	PREDICTED: pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [C...	[more]
XP_004140530.1	8.4e-240	96.03	pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucumis sati...	[more]
KAG6575304.1	2.1e-230	92.99	Pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic, partial [Cuc...	[more]
XP_022992260.1	4.6e-230	92.76	pyruvate dehydrogenase E1 component subunit alpha-3, chloroplastic [Cucurbita ma...	[more]

BLAST of Bhi12M002052 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TEC1	6.3e-241	96.26	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo var. makuwa OX...	[more]
A0A1S3CB66	6.3e-241	96.26	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis melo OX=3656 GN=LOC...	[more]
A0A0A0KFF3	4.1e-240	96.03	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucumis sativus OX=3659 GN=...	[more]
A0A6J1JV75	2.2e-230	92.76	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1EPB1	8.5e-230	92.76	Pyruvate dehydrogenase E1 component subunit alpha OS=Cucurbita moschata OX=3662 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001017	Dehydrogenase, E1 component	PFAM	PF00676	E1_dh	coord: 89..390 e-value: 3.5E-80 score: 269.2
None	No IPR available	GENE3D	3.40.50.970		coord: 43..410 e-value: 2.1E-121 score: 407.4
None	No IPR available	PANTHER	PTHR11516:SF58	PYRUVATE DEHYDROGENASE E1 COMPONENT SUBUNIT ALPHA	coord: 5..428
None	No IPR available	PANTHER	PTHR11516	PYRUVATE DEHYDROGENASE E1 COMPONENT, ALPHA SUBUNIT BACTERIAL AND ORGANELLAR	coord: 5..428
None	No IPR available	CDD	cd02000	TPP_E1_PDC_ADC_BCADC	coord: 86..384 e-value: 1.5284E-153 score: 434.616
IPR017597	Pyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit, subgroup y	TIGRFAM	TIGR03182	TIGR03182	coord: 81..401 e-value: 2.1E-139 score: 461.8
IPR029061	Thiamin diphosphate-binding fold	SUPERFAMILY	52518	Thiamin diphosphate-binding fold (THDP-binding)	coord: 75..403

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Bhi12G002052	Bhi12G002052	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi12M002052.exon3	Bhi12M002052.exon3	exon
Bhi12M002052.exon2	Bhi12M002052.exon2	exon
Bhi12M002052.exon1	Bhi12M002052.exon1	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi12M002052.utr3p1	Bhi12M002052.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Bhi12M002052	cds.Bhi12M002052_3	CDS
cds.Bhi12M002052	cds.Bhi12M002052_2	CDS
cds.Bhi12M002052	cds.Bhi12M002052	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi12M002052.utr5p1	Bhi12M002052.utr5p1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Bhi12M002052	Bhi12M002052-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006086	acetyl-CoA biosynthetic process from pyruvate
biological_process	GO:0006096	glycolytic process
cellular_component	GO:0043231	intracellular membrane-bounded organelle
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016624	oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor
molecular_function	GO:0004739	pyruvate dehydrogenase (acetyl-transferring) activity