Bhi08M000789 (mRNA) Wax gourd (B227) v1

Overview
NameBhi08M000789
TypemRNA
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptiontRNA (adenine(58)-N(1))-methyltransferase
Locationchr8: 30869186 .. 30871759 (-)
Sequence length1340
RNA-Seq ExpressionBhi08M000789
SyntenyBhi08M000789
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTCGTTAAACCAGCCTAACACCCCCGTCTGTGAGTTTCTCCTTCCAGCCGCCGCCGAAACCCGCAAGTACGAAGCAGTGGTGGGAGTTAACTTCCGGCGTTGCCTCCCCATCGTTTCTTACCCACTTACGGCGCCGGTGGTGCGGCACTCACAGCCCGACCCTCTCTTCCGGCGGTAAGTTTCACTCAGTCTAAATTCTCCTTCCAACTAGTTTATGGTTCTTTTTGTATATTCCGTATAAATTCGAAACTATTCGAACACTTTCTGGCTAGAATCAAGTCATGTATGTGTTAGACTTCTATTTAGATCCTTTTGACTTCAATTAAGGTGCTGTAATTTAGTGCATAACTCTCGTGTACTCTCTATTTGTTGTTTATATTCGATTCGAGGTGTTCGGATCAAATTTAAGGTTGCTTTAAGTAAGATTACTTGTGAAATTTTTTGGCTTATTCTAATGATTTATGGGATTTTGTACGATGTAATTGAAATGTTGGTTTACTCATAGTGTATTTATATAAATAATATCGATGAAGATGTCGACATGTTGACGAAAATTAATACCATATATACAACTCTTTATTCAAATGGGTTGTTTTCAAATTTCTGTTTTCAAACCATCAAATTATCCCCCATAAAGTCTAAATTTACCCTTGGCTTCTCTTCCTTCGTTTGCTTGTTAACTCCTCGTTTTCTTCTTGATTTAGCTTTTTCTCCTTCGTTTTTCTTTCTTCCCGTACCAGCCGCCACCAGCTTCCATCTTCCTCCTCGCCGGTGATTGTGTTCCCTCTGTAGCAACAGAACGCGAAACCCCCTACTCCAAATTCCACTCCGTAGGTAGTCCTTTATTTGAATGTTCCAGCACACTAGAATTTCCCAGTACTTAATTTTTTTCTGGCTGAATCTGCTTCATGATTGCTTACAGTGTTTCAATTCGTTTTGCTTCATTTTACACTTATTTTACCAATACTTGCAGCCCTTTGTTAGAAATACTTGGTTCTTCACTTTAAACATTTTCTGCATTGTTTCGATTTAGTTTCAATCGCGTTTACCGTTTTCTCGAGTTATATGATAATTTTCAGAAACGCTTCATGGAATTCTTCAAATAAGATGTTTGAGAACTTGAAACTTTTTCGAGATATTAATGATTTCAGATAGTAGTTTCAACTTTTCAACGCTTAGTGTCCATGATATGTTATTGTAATAGATCTATATTTTATTTGGTTGCTGTTTTTCCCCCAAATTTTGACAGAAGTTGATATTCTTGATTCTTAATGAGCTTTTGAAGACCAAGGATGTTGCCAACTGATGAGTTGAAAAAGATATCTTTCACCCGTTCTATAAGGGATGGAGATTTAGTCATTGTATATGAGAGACATGATATCATGAAGGCTGTGAAAGTATGTGAAGGGTCAACACTTCAGAATAGGTTCGGTGTGTTTAAACATACAGATTGGATTGGAAAGCCATTTGGGTCCAAGGTGTTGAGTAACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTTCTAAGCCACAGAACACAGATTCTATACATTGCAGATATTAGCTTTGTAATTATGTTCTTAGAAGTAGTTCCTGGCTGTTTAGTTCTAGAATCAGGGACAGGCAGTGGATCGTTGACAACGTCGTTAGCTAGGGCTGTAGCACCTACTGGACATGTCTATACGTTTGATTTTCATGAACAGAGAGCTTCCTCGGCGAGGTAGGAACATGGATCTATTGTATGTTCTCAAGTTACCCTTGCAGAGTAATAGTTCTGTGATTATTGTTAGATCTTGAGATAGTTTAAGAAGTAATACTTATTTGCTTGTTTCTCTCTTTTTTCAGAGAGGATTTTGAGAAGACTGGCTTGACAAGCCTTGTCACTGTGGGGGTTAGAGATATTCAAGGTCAGGGCTTTCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATCCCTTCAGCTGAGAAAATGCTGAAACAAGATGGCGTATTATGCTCCTTCTCTCCGTGTATCGAGCAAGTTCAGCGTTCAGCAGAAACTCTTCGATCTAAGTTTACAGGTACTTCCTGAATGACCCTATATGCCATTTAACTTTAGTTTTAGTGTGACCTTGATTTGTATTGTTCACCTTTTGTCATCTCTTTTGTGTAGATATAAGGACATTTGAGGTATTGCTTCGGACATACGAAGTTAAGGAAGGTAGAACAGAGAGTAACCAGGTTGATGGAGGTGGTTCTGTTGAGCTTCCTCCATGTAAGAGGCGGCTGAGATCCAAGGAATCAGATGACATGAACGATGATTCTAGTACGATGGTCATGGTTAGGCCGTGTAGTGAGGCCAGAGGTCACACCGGTTATCTAACATTCGCAAGACTTAGGTGCCTGTCTTAACAAAAATATAGTTCATTTTTCTAGCAAATTGGAAGTTTTGTATTCTTTTTCATCATTCTGGTACCTTGACACTCATTTCAGATTGTTCAATAGTTTTGTTTATATTCTTCTTTCTCATCTTGATATCAC

mRNA sequence

CTTCTCGTTAAACCAGCCTAACACCCCCGTCTGTGAGTTTCTCCTTCCAGCCGCCGCCGAAACCCGCAAGTACGAAGCAGTGGTGGGAGTTAACTTCCGGCGTTGCCTCCCCATCGTTTCTTACCCACTTACGGCGCCGGTGGTGCGGCACTCACAGCCCGACCCTCTCTTCCGGCGCCGCCACCAGCTTCCATCTTCCTCCTCGCCGGTGATTGTGTTCCCTCTGTAGCAACAGAACGCGAAACCCCCTACTCCAAATTCCACTCCGTAGACCAAGGATGTTGCCAACTGATGAGTTGAAAAAGATATCTTTCACCCGTTCTATAAGGGATGGAGATTTAGTCATTGTATATGAGAGACATGATATCATGAAGGCTGTGAAAGTATGTGAAGGGTCAACACTTCAGAATAGGTTCGGTGTGTTTAAACATACAGATTGGATTGGAAAGCCATTTGGGTCCAAGGTGTTGAGTAACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTTCTAAGCCACAGAACACAGATTCTATACATTGCAGATATTAGCTTTGTAATTATGTTCTTAGAAGTAGTTCCTGGCTGTTTAGTTCTAGAATCAGGGACAGGCAGTGGATCGTTGACAACGTCGTTAGCTAGGGCTGTAGCACCTACTGGACATGTCTATACGTTTGATTTTCATGAACAGAGAGCTTCCTCGGCGAGAGAGGATTTTGAGAAGACTGGCTTGACAAGCCTTGTCACTGTGGGGGTTAGAGATATTCAAGGTCAGGGCTTTCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATCCCTTCAGCTGAGAAAATGCTGAAACAAGATGGCGTATTATGCTCCTTCTCTCCGTGTATCGAGCAAGTTCAGCGTTCAGCAGAAACTCTTCGATCTAAGTTTACAGATATAAGGACATTTGAGGTATTGCTTCGGACATACGAAGTTAAGGAAGGTAGAACAGAGAGTAACCAGGTTGATGGAGGTGGTTCTGTTGAGCTTCCTCCATGTAAGAGGCGGCTGAGATCCAAGGAATCAGATGACATGAACGATGATTCTAGTACGATGGTCATGGTTAGGCCGTGTAGTGAGGCCAGAGGTCACACCGGTTATCTAACATTCGCAAGACTTAGGTGCCTGTCTTAACAAAAATATAGTTCATTTTTCTAGCAAATTGGAAGTTTTGTATTCTTTTTCATCATTCTGGTACCTTGACACTCATTTCAGATTGTTCAATAGTTTTGTTTATATTCTTCTTTCTCATCTTGATATCAC

Coding sequence (CDS)

ATGTTGCCAACTGATGAGTTGAAAAAGATATCTTTCACCCGTTCTATAAGGGATGGAGATTTAGTCATTGTATATGAGAGACATGATATCATGAAGGCTGTGAAAGTATGTGAAGGGTCAACACTTCAGAATAGGTTCGGTGTGTTTAAACATACAGATTGGATTGGAAAGCCATTTGGGTCCAAGGTGTTGAGTAACAAAGGTGGATTTGTTTACCTGTTAGCTCCAACTCCTGAATTATGGACTTTGGTTCTAAGCCACAGAACACAGATTCTATACATTGCAGATATTAGCTTTGTAATTATGTTCTTAGAAGTAGTTCCTGGCTGTTTAGTTCTAGAATCAGGGACAGGCAGTGGATCGTTGACAACGTCGTTAGCTAGGGCTGTAGCACCTACTGGACATGTCTATACGTTTGATTTTCATGAACAGAGAGCTTCCTCGGCGAGAGAGGATTTTGAGAAGACTGGCTTGACAAGCCTTGTCACTGTGGGGGTTAGAGATATTCAAGGTCAGGGCTTTCCTGAAGAGTTTGTTGGATTGGCAGATTCAGTTTTCTTGGATCTACCTCAACCTTGGTTGGCCATCCCTTCAGCTGAGAAAATGCTGAAACAAGATGGCGTATTATGCTCCTTCTCTCCGTGTATCGAGCAAGTTCAGCGTTCAGCAGAAACTCTTCGATCTAAGTTTACAGATATAAGGACATTTGAGGTATTGCTTCGGACATACGAAGTTAAGGAAGGTAGAACAGAGAGTAACCAGGTTGATGGAGGTGGTTCTGTTGAGCTTCCTCCATGTAAGAGGCGGCTGAGATCCAAGGAATCAGATGACATGAACGATGATTCTAGTACGATGGTCATGGTTAGGCCGTGTAGTGAGGCCAGAGGTCACACCGGTTATCTAACATTCGCAAGACTTAGGTGCCTGTCTTAA

Protein sequence

MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHTDWIGKPFGSKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSGSLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVGLADSVFLDLPQPWLAIPSAEKMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLLRTYEVKEGRTESNQVDGGGSVELPPCKRRLRSKESDDMNDDSSTMVMVRPCSEARGHTGYLTFARLRCLS
Homology
[Close]
BLAST of Bhi08M000789 vs. TAIR 10
Match: AT5G14600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 483.8 bits (1244), Expect = 1.0e-136
Identity = 239/318 (75.16%), Postives = 272/318 (85.53%), Query Frame = 0 

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHTDWIGKPFG 60
           MLPT+  K  SF R I DGDLVIVYERHD+MK VKV +   LQNRFGV+KH+DWIGKP G
Sbjct: 1   MLPTESKKAFSFKRCIEDGDLVIVYERHDVMKPVKVSKDGVLQNRFGVYKHSDWIGKPLG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSG 120
           +KV SNKG FVYLLAPTPELWTLVLSHRTQILYIADISFV+M+LEVVPGC+VLESGTGSG
Sbjct: 61  TKVFSNKGKFVYLLAPTPELWTLVLSHRTQILYIADISFVVMYLEVVPGCVVLESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SL+TSLARAVAPTGHVY+FDFHEQRA SAREDFEKTG++SLVTV VRDIQG+GFPE+  G
Sbjct: 121 SLSTSLARAVAPTGHVYSFDFHEQRAVSAREDFEKTGISSLVTVEVRDIQGEGFPEKLSG 180

Query: 181 LADSVFLDLPQPWLAIPSAEKMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLA+PSA KMLKQDGVLCSFSPCIEQVQR+ E LRS F +IRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAVPSAAKMLKQDGVLCSFSPCIEQVQRTCEVLRSDFIEIRTFEVLL 240

Query: 241 RTYEVKEGRTESNQVDGGGSVE----LPPCKRRLRSKESDDMNDD----SSTMVMVRPCS 300
           RTYEVKE + +++ + G    E    + PCKR+ RS E+D ++ D    +S++VM RPCS
Sbjct: 241 RTYEVKEVKMDTSSMVGESHEEDNEGMRPCKRKHRSNENDTVSQDNSSNASSVVMARPCS 300

Query: 301 EARGHTGYLTFARLRCLS 311
           EARGHTGYLTFARL+CL+
Sbjct: 301 EARGHTGYLTFARLQCLA 318

[Close]
BLAST of Bhi08M000789 vs. ExPASy Swiss-Prot
Match: Q80XC2 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Mus musculus OX=10090 GN=Trmt61a PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.0e-65
Identity = 138/298 (46.31%), Postives = 191/298 (64.09%), Query Frame = 0 

Query: 10  ISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHT-DWIGKPFGSKVLSNKG 69
           +++   I++GD  I+   H  M AV+V  G+  Q R GV +H+ D IG+PFGSKV+ ++G
Sbjct: 4   VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVICSRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSGSLTTSLAR 129
           G+VY+L PTPELWT+ L HRTQILY  DI+ + M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVGLADSVFLD 189
           +VAPTGH++T +FH+QRA  ARE+F++  L+  VTV  +D+   GF    V +AD+VFLD
Sbjct: 124 SVAPTGHLHTVEFHQQRADKAREEFQEHRLSQWVTVHTQDVCCSGF--GVVHVADAVFLD 183

Query: 190 LPQPWLAIPSAEKMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVK 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  FT++ T EVL + Y V+
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNVR 243

Query: 250 EGRTESNQVDGGGSVELPPCKRRLRSKESDDMNDDSSTMVMVRPCSEARGHTGYLTFA 305
                        +V LP       + E+ +M  D+S      P  E  GHTGYLTFA
Sbjct: 244 -------------TVSLPLPDLGANNLET-NMGSDASPFRSGTPMKETVGHTGYLTFA 285

[Close]
BLAST of Bhi08M000789 vs. ExPASy Swiss-Prot
Match: Q96FX7 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Homo sapiens OX=9606 GN=TRMT61A PE=1 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.0e-64
Identity = 137/298 (45.97%), Postives = 186/298 (62.42%), Query Frame = 0 

Query: 10  ISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHT-DWIGKPFGSKVLSNKG 69
           +++   I++GD  I+   H  M AV+V  G+  Q R GV +H+ D IG+PFGSKV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSGSLTTSLAR 129
           G+VY+L PTPELWTL L HRTQILY  DI+ + M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTLNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVGLADSVFLD 189
            +APTGH++T +FH+QRA  ARE+F++  +   VTV  +D+   GF    V  AD+VFLD
Sbjct: 124 TIAPTGHLHTVEFHQQRAEKAREEFQEHRVGRWVTVRTQDVCRSGFGVSHV--ADAVFLD 183

Query: 190 LPQPWLAIPSAEKMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVK 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L ++ F+++ T EVL + Y V+
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAARGFSELSTLEVLPQVYNVR 243

Query: 250 EGRTESNQVDGGGSVELPPCKRRLRSKESDDMNDDSSTMVMVRPCSEARGHTGYLTFA 305
                        +V LPP    L +        D+S      P  EA GHTGYLTFA
Sbjct: 244 -------------TVSLPP--PDLGTGTDGPAGSDTSPFRSGTPMKEAVGHTGYLTFA 284

[Close]
BLAST of Bhi08M000789 vs. ExPASy Swiss-Prot
Match: Q6AY46 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Rattus norvegicus OX=10116 GN=Trmt61a PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.5e-63
Identity = 135/298 (45.30%), Postives = 190/298 (63.76%), Query Frame = 0 

Query: 10  ISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHT-DWIGKPFGSKVLSNKG 69
           +++   I++GD  I+   H  M AV+V  G+  Q R GV +H+ D IG+PFGSKV  ++G
Sbjct: 4   VAYEELIKEGDTAILSLGHGSMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCSRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSGSLTTSLAR 129
           G+VY+L PTPELWT+ L HRTQILY  DI+ + M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTVNLPHRTQILYSTDIALITMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVGLADSVFLD 189
           ++APTGH++T +FH+QRA  ARE+F++  ++  VTV  +D+   GF    V +AD+VFLD
Sbjct: 124 SIAPTGHLHTVEFHQQRADKAREEFQQHRVSQWVTVHTQDVCRSGF--GVVHVADAVFLD 183

Query: 190 LPQPWLAIPSAEKMLK-QDGVLCSFSPCIEQVQRSAETLRSK-FTDIRTFEVLLRTYEVK 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  FT++ T EVL + Y V 
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAAHGFTELSTLEVLPQVYNV- 243

Query: 250 EGRTESNQVDGGGSVELPPCKRRLRSKESDDMNDDSSTMVMVRPCSEARGHTGYLTFA 305
             RT S  +   G+ +L             ++  D++      P  E  GHTGYLTFA
Sbjct: 244 --RTVSLPLPDLGADDL-----------EGNVGSDATPFRSGTPMKETVGHTGYLTFA 285

[Close]
BLAST of Bhi08M000789 vs. ExPASy Swiss-Prot
Match: A6H791 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Bos taurus OX=9913 GN=TRMT61A PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 4.1e-61
Identity = 136/298 (45.64%), Postives = 183/298 (61.41%), Query Frame = 0 

Query: 10  ISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHT-DWIGKPFGSKVLSNKG 69
           +++   I++GD  I+   H  M AV+V  G+  Q R GV +H+ D IG+PFGSKV   +G
Sbjct: 4   VAYEELIKEGDTAILSLGHGAMVAVRVQRGAQTQTRHGVLRHSVDLIGRPFGSKVTCGRG 63

Query: 70  GFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSGSLTTSLAR 129
           G+VY+L PTPELWTL L HRTQILY  DI+ + M LE+ PG +V ESGTGSGS++ ++ R
Sbjct: 64  GWVYVLHPTPELWTLNLPHRTQILYSTDIALLTMMLELRPGSVVCESGTGSGSVSHAIIR 123

Query: 130 AVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVGLADSVFLD 189
            +APTGH++T +FH+QRA  ARE+F++  +   VTV  +D+   GF    V  AD+VFLD
Sbjct: 124 TIAPTGHLHTVEFHQQRAERAREEFQEHRVGRWVTVLNQDVCRSGFGVSHV--ADAVFLD 183

Query: 190 LPQPWLAIPSAEKMLK-QDGVLCSFSPCIEQVQRSAETLRS-KFTDIRTFEVLLRTYEVK 249
           +P PW A+  A   LK + G  CSFSPCIEQVQR+ + L +  F+++ T EVL + Y V 
Sbjct: 184 IPSPWEAVGHAWDALKVEGGRFCSFSPCIEQVQRTCQALAACGFSELSTLEVLPQVYNV- 243

Query: 250 EGRTESNQVDGGGSVELPPCKRRLRSKESDDMNDDSSTMVMVRPCSEARGHTGYLTFA 305
             RT S  V   G+   P                D++      P  E  GHTGYLTFA
Sbjct: 244 --RTVSLPVPDLGARPGP----------------DAAPFRSGTPMKETVGHTGYLTFA 280

[Close]
BLAST of Bhi08M000789 vs. ExPASy Swiss-Prot
Match: Q86JJ0 (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit trmt61a OS=Dictyostelium discoideum OX=44689 GN=trmt61a PE=1 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 7.6e-60
Identity = 130/300 (43.33%), Postives = 187/300 (62.33%), Query Frame = 0 

Query: 16  IRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHTDWIGKPFGSKVLSNKG-GFVYLL 75
           I++GD V++Y   D M  + +   +   ++FG ++H + IGK +GSK+ S+ G GF +++
Sbjct: 8   IKEGDRVVMYNGKDNMAVLTMESNNVYNSKFGSYRHKNIIGKEYGSKLSSDNGNGFCHVI 67

Query: 76  APTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSGSLTTSLARAVAPTG 135
           A TPELW++ L HRTQIL+  DIS +I  LE+  G   +ESGTGSGSL++S+AR +AP G
Sbjct: 68  AMTPELWSITLDHRTQILFNLDISTIIFNLELKNGSRAVESGTGSGSLSSSIARTIAPKG 127

Query: 136 HVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQG-QGFPEEFVGL-ADSVFLDLPQP 195
           H++TF+FHE+R   AR+DF+  GL   +TV  RD  G +GF  + +    D+VFLDLP P
Sbjct: 128 HLFTFEFHEERVKFARKDFKDNGLDQYITVTHRDACGKEGFLRQDINNDIDAVFLDLPSP 187

Query: 196 WLAIPSAEKMLKQDGVLCSFSPCIEQVQRSAETLR-SKFTDIRTFEVLLRTY-----EVK 255
           W AI +A  ++    +LCSFSPCIEQVQ +   L  SKF +I+T EVL+RT+     E +
Sbjct: 188 WDAIENAIAVMHDGSMLCSFSPCIEQVQNTCLKLADSKFQEIKTIEVLIRTFDTRLQEYE 247

Query: 256 EGRTESNQVDGGGSVELPPCKRRLRSK-ESDDMNDDSSTMVMVRPCSEARGHTGYLTFAR 306
           E    +  +D   +          R K E   +       ++ +P +EARGHTGYLTFAR
Sbjct: 248 ELNLTNPYIDNNNNNNNNNNIEENRGKFEIGGIEGLKKDKLLSKPFTEARGHTGYLTFAR 307

[Close]
BLAST of Bhi08M000789 vs. ExPASy TrEMBL
Match: A0A0A0LYQ8 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3 SV=1)

HSP 1 Score: 604.7 bits (1558), Expect = 2.1e-169
Identity = 297/310 (95.81%), Postives = 305/310 (98.39%), Query Frame = 0 

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHTDWIGKPFG 60
           MLPTDELK ISFTRSIRDGDLVIVYERHDIMKAVKVC+GSTLQNRFGVFKH+DWIGKPFG
Sbjct: 396 MLPTDELKSISFTRSIRDGDLVIVYERHDIMKAVKVCDGSTLQNRFGVFKHSDWIGKPFG 455

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSG 120
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLE+VPGCLVLESGTGSG
Sbjct: 456 SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 515

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG
Sbjct: 516 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 575

Query: 181 LADSVFLDLPQPWLAIPSAEKMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAE+MLKQDG+LCSFSPCIEQVQRS ETLRSKFTDIRTFEVLL
Sbjct: 576 LADSVFLDLPQPWLAIPSAERMLKQDGMLCSFSPCIEQVQRSVETLRSKFTDIRTFEVLL 635

Query: 241 RTYEVKEGRTESNQVDGGGSVELPPCKRRLRSKESDDMNDDSSTMVMVRPCSEARGHTGY 300
           RTYEVKEGRTESNQVDGGGSV LPPCKRRLRSKESDDM+++SST VMVRPCSEARGHTGY
Sbjct: 636 RTYEVKEGRTESNQVDGGGSVGLPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 695

Query: 301 LTFARLRCLS 311
           LTFARLRCLS
Sbjct: 696 LTFARLRCLS 705

[Close]
BLAST of Bhi08M000789 vs. ExPASy TrEMBL
Match: A0A1S3C9I4 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103498313 PE=3 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.4e-168
Identity = 296/310 (95.48%), Postives = 304/310 (98.06%), Query Frame = 0 

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHTDWIGKPFG 60
           MLPTDELK + FTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKH+DWIGKPFG
Sbjct: 7   MLPTDELKNLYFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 66

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSG 120
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLE+VPGCLVLESGTGSG
Sbjct: 67  SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 126

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG
Sbjct: 127 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 186

Query: 181 LADSVFLDLPQPWLAIPSAEKMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAE+MLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 187 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 246

Query: 241 RTYEVKEGRTESNQVDGGGSVELPPCKRRLRSKESDDMNDDSSTMVMVRPCSEARGHTGY 300
           RTYEVKEGRTESN+VDGGGSV LPPCKRRLRSKESDDM+++SST VMVRPCSEARGHTGY
Sbjct: 247 RTYEVKEGRTESNEVDGGGSVALPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 306

Query: 301 LTFARLRCLS 311
           LTFARL CLS
Sbjct: 307 LTFARLGCLS 316

[Close]
BLAST of Bhi08M000789 vs. ExPASy TrEMBL
Match: A0A5A7TJ63 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1734G00040 PE=4 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.4e-168
Identity = 296/310 (95.48%), Postives = 304/310 (98.06%), Query Frame = 0 

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHTDWIGKPFG 60
           MLPTDELK + FTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKH+DWIGKPFG
Sbjct: 58  MLPTDELKNLYFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 117

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSG 120
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLE+VPGCLVLESGTGSG
Sbjct: 118 SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 177

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG
Sbjct: 178 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 237

Query: 181 LADSVFLDLPQPWLAIPSAEKMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAE+MLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 238 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 297

Query: 241 RTYEVKEGRTESNQVDGGGSVELPPCKRRLRSKESDDMNDDSSTMVMVRPCSEARGHTGY 300
           RTYEVKEGRTESN+VDGGGSV LPPCKRRLRSKESDDM+++SST VMVRPCSEARGHTGY
Sbjct: 298 RTYEVKEGRTESNEVDGGGSVALPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 357

Query: 301 LTFARLRCLS 311
           LTFARL CLS
Sbjct: 358 LTFARLGCLS 367

[Close]
BLAST of Bhi08M000789 vs. ExPASy TrEMBL
Match: A0A1S3C8X5 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103498313 PE=4 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.4e-168
Identity = 296/310 (95.48%), Postives = 304/310 (98.06%), Query Frame = 0 

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHTDWIGKPFG 60
           MLPTDELK + FTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKH+DWIGKPFG
Sbjct: 58  MLPTDELKNLYFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHSDWIGKPFG 117

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSG 120
           SK LSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLE+VPGCLVLESGTGSG
Sbjct: 118 SKALSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLVLESGTGSG 177

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG
Sbjct: 178 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 237

Query: 181 LADSVFLDLPQPWLAIPSAEKMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAE+MLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 238 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 297

Query: 241 RTYEVKEGRTESNQVDGGGSVELPPCKRRLRSKESDDMNDDSSTMVMVRPCSEARGHTGY 300
           RTYEVKEGRTESN+VDGGGSV LPPCKRRLRSKESDDM+++SST VMVRPCSEARGHTGY
Sbjct: 298 RTYEVKEGRTESNEVDGGGSVALPPCKRRLRSKESDDMDNESSTTVMVRPCSEARGHTGY 357

Query: 301 LTFARLRCLS 311
           LTFARL CLS
Sbjct: 358 LTFARLGCLS 367

[Close]
BLAST of Bhi08M000789 vs. ExPASy TrEMBL
Match: A0A6J1H9F4 (tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111461754 PE=3 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.4e-165
Identity = 288/310 (92.90%), Postives = 305/310 (98.39%), Query Frame = 0 

Query: 1   MLPTDELKKISFTRSIRDGDLVIVYERHDIMKAVKVCEGSTLQNRFGVFKHTDWIGKPFG 60
           MLPTD+LKKISFTRSIRDGDLVIVYERHD+MKAVKVCE ST QNRFG+FKH+DWIGKPFG
Sbjct: 1   MLPTDDLKKISFTRSIRDGDLVIVYERHDVMKAVKVCENSTFQNRFGMFKHSDWIGKPFG 60

Query: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEVVPGCLVLESGTGSG 120
           SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLE+VPGCL+LESGTGSG
Sbjct: 61  SKVLSNKGGFVYLLAPTPELWTLVLSHRTQILYIADISFVIMFLEIVPGCLILESGTGSG 120

Query: 121 SLTTSLARAVAPTGHVYTFDFHEQRASSAREDFEKTGLTSLVTVGVRDIQGQGFPEEFVG 180
           SLTTSLARAVAPTGHVYTFDFHEQRA+SAREDFEKTGLTSLVTVGVRD+QGQGFPEEFVG
Sbjct: 121 SLTTSLARAVAPTGHVYTFDFHEQRAASAREDFEKTGLTSLVTVGVRDVQGQGFPEEFVG 180

Query: 181 LADSVFLDLPQPWLAIPSAEKMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240
           LADSVFLDLPQPWLAIPSAE+MLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL
Sbjct: 181 LADSVFLDLPQPWLAIPSAERMLKQDGVLCSFSPCIEQVQRSAETLRSKFTDIRTFEVLL 240

Query: 241 RTYEVKEGRTESNQVDGGGSVELPPCKRRLRSKESDDMNDDSSTMVMVRPCSEARGHTGY 300
           RTYEV+EG+TES+QV+GGGSVELPPCK+R+RSKE++D N DSST VMVRPCSEARGHTGY
Sbjct: 241 RTYEVREGKTESSQVEGGGSVELPPCKKRVRSKEANDTNGDSST-VMVRPCSEARGHTGY 300

Query: 301 LTFARLRCLS 311
           LTFARLRCLS
Sbjct: 301 LTFARLRCLS 309

The following BLAST results are available for this feature:
BLAST of Bhi08M000789 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G14600.11.0e-13675.16S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
BLAST of Bhi08M000789 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q80XC26.0e-6546.31tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Mus muscu... [more]
Q96FX73.0e-6445.97tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Homo sapi... [more]
Q6AY461.5e-6345.30tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Rattus no... [more]
A6H7914.1e-6145.64tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A OS=Bos tauru... [more]
Q86JJ07.6e-6043.33tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit trmt61a OS=Dictyoste... [more]
BLAST of Bhi08M000789 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LYQ82.1e-16995.81(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G555080 PE=3... [more]
A0A1S3C9I41.4e-16895.48tRNA (adenine(58)-N(1))-methyltransferase OS=Cucumis melo OX=3656 GN=LOC10349831... [more]
A0A5A7TJ631.4e-16895.48tRNA (adenine(58)-N(1))-methyltransferase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3C8X51.4e-16895.48tRNA (adenine(58)-N(1))-methyltransferase OS=Cucumis melo OX=3656 GN=LOC10349831... [more]
A0A6J1H9F42.4e-16592.90tRNA (adenine(58)-N(1))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PIRSFPIRSF017269GCD14coord: 10..309
e-value: 2.9E-98
score: 326.1
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PFAMPF08704GCD14coord: 69..305
e-value: 6.7E-98
score: 327.3
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PANTHERPTHR12133TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASEcoord: 2..307
IPR014816tRNA (1-methyladenosine) methyltransferase catalytic subunit Gcd14PROSITEPS51620SAM_TRM61coord: 19..307
score: 97.772873
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 78..308
e-value: 4.8E-80
score: 270.2
NoneNo IPR availableGENE3D3.10.330.20coord: 10..77
e-value: 6.5E-26
score: 92.2
NoneNo IPR availablePIRSRPIRSR017269-1PIRSR017269-1coord: 47..251
e-value: 1.8E-41
score: 139.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 249..283
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 266..280
NoneNo IPR availablePANTHERPTHR12133:SF2TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE CATALYTIC SUBUNIT TRMT61Acoord: 2..307
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 15..306

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Bhi08G000789Bhi08G000789gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi08M000789.exon5Bhi08M000789.exon5exon
Bhi08M000789.exon4Bhi08M000789.exon4exon
Bhi08M000789.exon3Bhi08M000789.exon3exon
Bhi08M000789.exon2Bhi08M000789.exon2exon
Bhi08M000789.exon1Bhi08M000789.exon1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi08M000789.utr3p1Bhi08M000789.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Bhi08M000789cds.Bhi08M000789_3CDS
cds.Bhi08M000789cds.Bhi08M000789_2CDS
cds.Bhi08M000789cds.Bhi08M000789CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi08M000789.utr5p3Bhi08M000789.utr5p3five_prime_UTR
Bhi08M000789.utr5p2Bhi08M000789.utr5p2five_prime_UTR
Bhi08M000789.utr5p1Bhi08M000789.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Bhi08M000789Bhi08M000789-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
cellular_component GO:0031515 tRNA (m1A) methyltransferase complex
molecular_function GO:0016429 tRNA (adenine-N1-)-methyltransferase activity