Tan0022676 (gene) Snake gourd v1

Overview
NameTan0022676
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionpolygalacturonase inhibitor-like
LocationLG03: 48357907 .. 48361787 (-)
RNA-Seq ExpressionTan0022676
SyntenyTan0022676
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCTTCCTTCTCCTAATCTCAAACTCCCATGGCTTCCCTTCTTCCTCCTCTTCTTCATCTTCACCATTTCTTTTGCAGAACTCTGCAACCCAAATGACAAAAAAGCCCTCCTCAACATCAAAAATGCCTTCAACAACCCCTACATCCTCTCCTCTTGGCACCCTGAAACCGATTGCTGTGATTGGTACTCTGTGGACTGCGATCCAACTTCCCACCGGGTAATCTCCCTGGCCATCGTCTCCGGCCAACTCTCCGGCCAAATCCCACCGGACGTCGGCGACCTTCCGCTTCTACAAACCCTCATGTTCCACAAGGTCCCAAATCTCGCCGGCCGGATCCAACCCGCCATCGCAAAGCTTCACAGCCTCAAATTCTTGTGGATTAACTGGACCAATGTTTCTGGCTCAATCCCTGATTTCCTCAGCAGCCTCACGAATCTCAACCACCCTTTCCTTTAACAATCTCTCTGGAACCATCCCAAGTTCCCTCTCCCAACTCCCAAATCTTGCCGCTCTCCAGTTGGACCGAAACAAATTAACCGGACCGATCCCGGACTCGTTCGGGTACTTTAAGGCTGAAATTCAGTACCTTTATCTTTCCCACAACCAACTTTCAGGGAAAATTCCAGCATCCTTGTCGAGAATGAACTTCACTTTGATTGATTTGTCGAGAAACAAGCTCGTAGGCGATGCGTTTATGATTTTTGGTGCGAATAAAACCGTCCAGATTGTTGATTTGTCGAGAAATCTGTTGGAGTTTAACTTGTCGGAGGTTGTGTTGGGTTTTGTGTGGAGACAAAATAAAAGGCCAATGGGCCAAGGCCCATTTGGATATTAGGTTTTTGTAAACACATATATGATGTTTTGTGACGGAGGAAAAAACTGGGTGTGACCAAAATAGCATTTGTGAGAGAAAAAGAGTTTTTCTCTGGTGGGGTTGAAGGTTTTTCTTGATTATCTCTTGTGATACTTGAAAGTCAATAGCCATTGTTGAAATTTGTGAGAGGGTTAGTTGTGTGGCCTACCAATCGAGAGGCTCTTTTTGTAATCTCCCATGAACTGCGGATTCTGCTGCGAAAGACTGACGTAATCCCAAGTTTGAGGGCGACCAGAAGATAAATTTCGGTGTCGTTCTCTTTTCCTATCTCGCTATATTTAATATTTTGTGACTTCTAGTTTATTGGTTGACTTATCACGTGTGGAAGGGAGATTTAATATTATAATAATTTTATTGTGATTATTATTATCATTATTTTTCTCCCAACAAGTGGTATCGTAGCTTTTTGGTTGAGAGTCATGGGATACGTGGAGCCAACAAGTTTTGATGGAGTCATGAAGTTTAATGAGGACAATTTTGAATATTGGAAGATGATGGTCAAAGACTACTTGATCGTAAAAAGGCTGGGTAAGGCTTTGAAAAAGAGACCAGGAAGGAATGAGAGATGGAGATTGGGAGATTCTTGATGAAGAAGCGATTTGCTACAATCGTAATGTAATGCATCGATGGGCGTGGCGATTAGGTAGCCAATGAGACAACTGCGATTGAGTTAATGAAAGCGCTTGCAAACAGGTATGAAAAATCCTGCACAAATAATAAATAATAAGATCTATCTGCTTAGGAAGTTTTTCAATATGCGTATGGTGAGGATGATTCTTGTGAATTCCTATATAAATGAGGTAACCACTTTGATAAATCAGTTGAAATCAGTTAGAATAGAGTTCACTGATGAAGTAAATGCTATTCTGTTATTAACCTCTTTGCCTGATAGTTGGAAAACCATGAAGACAGCAGTGTGTAATTCAGTAGGTGAAAATACTCTACACTTTTCTGGTGTTTGTAATTTAGTCATAGTTGAGGAAATTCGTAGAAAAGATAGTAGTAAAGAGAGTCTACTATAGGATCTGCTCTGGTTGTGACTAAGGGAAAAGAGAAGATTAATGAGGAAAATACATCGAGTAGCAATAGGAAGAAGTGGAATAATATGAATAAAGAGATAGAATGATTTTACTGTCACAAGAAAGGTCATTTTAAGAGTCAGTGTAGAAAATTGAAAGAGGATCTGGAGAGGAGACAAAATCAAGAGGCAAATGTAGTGCTAGATGCATGTGTGCGTGTTGGGAGTGACACAGAGAGTAGTAATAGTTCATCCGAGTGGATTTTGGGCCAGTAGCAGCCTTGCTCACATAACTTCAGATAGGAGTTTGTTCACATCCTTCACGGGGAGGTCGTCATGGTTTAGTGAGGATGGGGAATGGTGAAATATCCAAGACCAATGGGATTGGAGAGGTTAGTCCGGAGACGCGAATGTGGGAATAATTTGAGTCTGCGAGATGTCGATTTGTGCCTGTGTCAAGATGAATCTTATCTCTCTTGGCAAGTTGGCGGATGATGGCTACATTTGTGAGTTTGGCGAAGCTTGATGTAAACTTAGGTCGAGATCCCGTGTAGTGGCGATTGGCCACGAAAAATCTACATTATACGATGTCGGTTAAATGTTGCCAAGAAAAGTTTGAAGAGACGATGGATGCCGATTAAAGTTGCGAGATGGTTATAGTAGAGGTGCAGTTGAGCCAAGAATGAATGATAGCCAAGTTTGATCGATCGATCGAGATCCTTGATTCAAGAACAATTGGGAAGTCTAGGGAGAAAATTGATGGCTATCATGAACCCCCAATTGTCAGACGGAAAGTTGAATTGAAGAAGTCGCCTAGGCGAGTGGAGGCATCAAAGTGGAAGGCCAAAGCAGTTGCTAAGGTCAAAGGTAAAGTCTCTAAATTGGCAGCAAGCTTGAATAGAGGATTCAAGCCCTTCTTAGAGTGTATCTTCTTCAGGAACAATGGTTTAGGGTTGAAGAAGATGTTAGTTGGTACCACTTAGCTGAAGTGGGAGTACGTGTCTGTCTTTTTCGTCTAGGAGACAGATCCACGTAGTGAGCCCATACCTCGGGACAAACACAGATGCACTCACGCTATATTCGATGGTTGCCTGGTTTTTGTGAGTTTATTGTGTTTTCAGGTATCTTTTATTTGAGCCTTGGAGTTTGCCAAGAAGATCAGAAATAACAGTTGTAGTGGGAGTTGTGCCTTGGAGTTTACCAAGGTGATCAAAGTTTTGTTGCAGTATGAGGCAAGATTGAAGTTTTGTCTCCAAGTGGGAGATTGTTGGGTTTTGTGTGGAGACAAAATAAAAGGCCAATGGGCCAAGGCCCATTTGGATATTAGGTTTTTGTAAACACACATATATATATATATATATATGATGTTTTGTGTGTAGCCACCAACAAACAGAGAGGGAAAAACTGGGTGTGACCAAAATAGCATTTGTGAGAGAAAAAGAGTTTTTCTCTGGTGGGGTTGAAAGTTTTTCTTGATTATCTCAAGCTATCGGGTTGTGATACTTGTAAGTCAATAGCCATTGTTGGGCTATTGAGGTTAGTTTGTGTACCGAATTGAGAGCTCTTTTGTAATCTCCCATTATAGTAGAACACTGCATTGTAGAAGGCGGGCGTAGTCCCAAGTTTAGAAGCGAACAGAGATAAATTTCGGTGTCGTTCTCTTTTCCCTATCTCGTTATATTTAATATTTTGTGACTCTAGTTTATTGGTTTATAATAATTTTATTGTGATTATTATTATCATTATTTTTTCTCCCAACAGGTTGTGTTTCCTCAGAGCTTGACTTCGTTGGATCTTAATCATAATAAGGTCTTTGGGAGTTTTTCGCCGGAGTTGACGAAACTGAATTTTCAGTATTTGAATGTCAGTTATAATCGGCTTTGTCGTGAAATTCCTGTGGGTGGGCAGTTGCAGAGTTTTGGTGTCTCATCTTATTTTCATAATAAGTGTTTATGTGGCGCACCGCTTATTGGATGCAAGTAA

mRNA sequence

ATGGCTCTTCCTTCTCCTAATCTCAAACTCCCATGGCTTCCCTTCTTCCTCCTCTTCTTCATCTTCACCATTTCTTTTGCAGAACTCTGCAACCCAAATGACAAAAAAGCCCTCCTCAACATCAAAAATGCCTTCAACAACCCCTACATCCTCTCCTCTTGGCACCCTGAAACCGATTGCTGTGATTGGTACTCTGTGGACTGCGATCCAACTTCCCACCGGGTAATCTCCCTGGCCATCGTCTCCGGCCAACTCTCCGGCCAAATCCCACCGGACGTCGGCGACCTTCCGCTTCTACAAACCCTCATGTTCCACAAGGTCCCAAATCTCGCCGGCCGGATCCAACCCGCCATCGCAAAGCTTCACAGCCTCAAATTCTTTTCCCTCTCCCAACTCCCAAATCTTGCCGCTCTCCAGTTGGACCGAAACAAATTAACCGGACCGATCCCGGACTCGTTCGGGTACTTTAAGGCTGAAATTCAGTACCTTTATCTTTCCCACAACCAACTTTCAGGGAAAATTCCAGCATCCTTGTCGAGAATGAACTTCACTTTGATTGATTTGTCGAGAAACAAGCTCGTAGGCGATGCGTTTATGATTTTTGGTGCGAATAAAACCGTCCAGATTGTTGATTTGTCGAGAAATCTGTTGGAGTTTAACTTGTCGGAGGTTGTGTTTCCTCAGAGCTTGACTTCGTTGGATCTTAATCATAATAAGGTCTTTGGGAGTTTTTCGCCGGAGTTGACGAAACTGAATTTTCAGTATTTGAATGTCAGTTATAATCGGCTTTGTCGTGAAATTCCTGTGGGTGGGCAGTTGCAGAGTTTTGGTGTCTCATCTTATTTTCATAATAAGTGTTTATGTGGCGCACCGCTTATTGGATGCAAGTAA

Coding sequence (CDS)

ATGGCTCTTCCTTCTCCTAATCTCAAACTCCCATGGCTTCCCTTCTTCCTCCTCTTCTTCATCTTCACCATTTCTTTTGCAGAACTCTGCAACCCAAATGACAAAAAAGCCCTCCTCAACATCAAAAATGCCTTCAACAACCCCTACATCCTCTCCTCTTGGCACCCTGAAACCGATTGCTGTGATTGGTACTCTGTGGACTGCGATCCAACTTCCCACCGGGTAATCTCCCTGGCCATCGTCTCCGGCCAACTCTCCGGCCAAATCCCACCGGACGTCGGCGACCTTCCGCTTCTACAAACCCTCATGTTCCACAAGGTCCCAAATCTCGCCGGCCGGATCCAACCCGCCATCGCAAAGCTTCACAGCCTCAAATTCTTTTCCCTCTCCCAACTCCCAAATCTTGCCGCTCTCCAGTTGGACCGAAACAAATTAACCGGACCGATCCCGGACTCGTTCGGGTACTTTAAGGCTGAAATTCAGTACCTTTATCTTTCCCACAACCAACTTTCAGGGAAAATTCCAGCATCCTTGTCGAGAATGAACTTCACTTTGATTGATTTGTCGAGAAACAAGCTCGTAGGCGATGCGTTTATGATTTTTGGTGCGAATAAAACCGTCCAGATTGTTGATTTGTCGAGAAATCTGTTGGAGTTTAACTTGTCGGAGGTTGTGTTTCCTCAGAGCTTGACTTCGTTGGATCTTAATCATAATAAGGTCTTTGGGAGTTTTTCGCCGGAGTTGACGAAACTGAATTTTCAGTATTTGAATGTCAGTTATAATCGGCTTTGTCGTGAAATTCCTGTGGGTGGGCAGTTGCAGAGTTTTGGTGTCTCATCTTATTTTCATAATAAGTGTTTATGTGGCGCACCGCTTATTGGATGCAAGTAA

Protein sequence

MALPSPNLKLPWLPFFLLFFIFTISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDWYSVDCDPTSHRVISLAIVSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHSLKFFSLSQLPNLAALQLDRNKLTGPIPDSFGYFKAEIQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCREIPVGGQLQSFGVSSYFHNKCLCGAPLIGCK
Homology
BLAST of Tan0022676 vs. ExPASy Swiss-Prot
Match: Q05091 (Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 4.1e-103
Identity = 193/323 (59.75%), Postives = 225/323 (69.66%), Query Frame = 0

Query: 12  WLPFFLLF-FIFTISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDWYSVDCDP 71
           +L   LLF  +   + ++LCNP+DKK LL IK AF +PY+L+SW  +TDCCDWY V CD 
Sbjct: 8   FLSLTLLFSSVLNPALSDLCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCVTCDS 67

Query: 72  TSHRVISLAIVSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHSLKFF--- 131
           T++R+ SL I +GQ+SGQIP  VGDLP L+TL FHK PNL G IQPAIAKL  LK     
Sbjct: 68  TTNRINSLTIFAGQVSGQIPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKSLRLS 127

Query: 132 ----------------------------------SLSQLPNLAALQLDRNKLTGPIPDSF 191
                                             SLS+LPNL AL+LDRNKLTG IP SF
Sbjct: 128 WTNLSGSVPDFLSQLKNLTFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGHIPISF 187

Query: 192 GYFKAEIQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANKTVQIVDLS 251
           G F   +  LYLSHNQLSG IP S ++M+FT IDLSRNKL GDA +IFG NKT QIVDLS
Sbjct: 188 GQFIGNVPDLYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTTQIVDLS 247

Query: 252 RNLLEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCREIPVGGQL 297
           RNLLEFNLS+V FP SLTSLD+NHNK++GS   E T+LNFQ+LNVSYNRLC +IPVGG+L
Sbjct: 248 RNLLEFNLSKVEFPTSLTSLDINHNKIYGSIPVEFTQLNFQFLNVSYNRLCGQIPVGGKL 307

BLAST of Tan0022676 vs. ExPASy Swiss-Prot
Match: A7PW81 (Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 8.3e-96
Identity = 180/309 (58.25%), Postives = 208/309 (67.31%), Query Frame = 0

Query: 25  SFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDWYSVDCDPTSHRVISLAIVSGQ 84
           S +E CNP DKK LL IK A +NPYIL+SW+P TDCC WY V+CD T+HR+ SL I SGQ
Sbjct: 25  SLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDCCGWYCVECDLTTHRINSLTIFSGQ 84

Query: 85  LSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHSLK---------------FF-- 144
           LSGQIP  VGDLP L+TL+F K+ NL G+I PAIAKL  LK               FF  
Sbjct: 85  LSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVRLSWTNLSGPVPAFFSE 144

Query: 145 --------------------SLSQLPNLAALQLDRNKLTGPIPDSFGYFKAEIQYLYLSH 204
                               SLS LPNL AL LDRN LTGPIPDSFG F      L+LSH
Sbjct: 145 LKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNHLTGPIPDSFGKFAGSTPGLHLSH 204

Query: 205 NQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANKTVQIVDLSRNLLEFNLSEVVFP 264
           NQLSGKIP S    +  ++DLSRNKL GD  + F ANK+ QIVD SRNL +F+LS V FP
Sbjct: 205 NQLSGKIPYSFRGFDPNVMDLSRNKLEGDLSIFFNANKSTQIVDFSRNLFQFDLSRVEFP 264

Query: 265 QSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCREIPVGGQLQSFGVSSYFHNKCL 297
           +SLTSLDL+HNK+ GS    +T L+ Q+LNVSYNRLC +IPVGG+LQSF   SYFHN+CL
Sbjct: 265 KSLTSLDLSHNKIAGSLPEMMTSLDLQFLNVSYNRLCGKIPVGGKLQSFDYDSYFHNRCL 324

BLAST of Tan0022676 vs. ExPASy Swiss-Prot
Match: Q9M5J9 (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 7.7e-86
Identity = 164/320 (51.25%), Postives = 204/320 (63.75%), Query Frame = 0

Query: 16  FLLFFIFTISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDWYSVDC-DPT-SH 75
           FL  F+ T    +LCN NDK  LL IK + NNPY L+SW P+TDCC WY ++C D T +H
Sbjct: 11  FLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDATVNH 70

Query: 76  RVISLAIVSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHSLKFF------ 135
           RV +L I SGQ+SGQIP +VGDLP L+TL+F K+ NL G IQP IAKL +L+        
Sbjct: 71  RVTALTIFSGQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLRLSWTN 130

Query: 136 -------------------------------SLSQLPNLAALQLDRNKLTGPIPDSFGYF 195
                                          SLS LP + AL+L RNKLTG IP+SFG F
Sbjct: 131 LTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPESFGSF 190

Query: 196 KAEIQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANKTVQIVDLSRNL 255
              +  L LSHNQLSG IP SL  ++F  IDLSRNKL GDA M+FG+NKT   +DLSRN+
Sbjct: 191 PGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSIDLSRNM 250

Query: 256 LEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCREIPVGGQLQSF 297
            +F++S+V  P++L  LDLNHN + G+   + T+   Q+ NVSYN+LC  IP GG+LQ+F
Sbjct: 251 FQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIPTGGKLQTF 310

BLAST of Tan0022676 vs. ExPASy Swiss-Prot
Match: Q9M5J8 (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 307.8 bits (787), Expect = 1.4e-82
Identity = 165/326 (50.61%), Postives = 204/326 (62.58%), Query Frame = 0

Query: 13  LPFFLLF--FIFTISFA-ELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDWYSVDC- 72
           +  FLL    + T S A +LC+ +DK  LL IK + NNPY L+SW P+TDCC WY ++C 
Sbjct: 5   MTLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECG 64

Query: 73  DPT-SHRVISLAIVSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHSLKFF 132
           D T +HRV SL I  G++SGQIPP+VGDLP L +L+F K+ NL G IQP IAKL +L F 
Sbjct: 65  DATVNHRVTSLIIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFL 124

Query: 133 -------------------------------------SLSQLPNLAALQLDRNKLTGPIP 192
                                                SLS L  L  L+L RNKLTGPIP
Sbjct: 125 RLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIP 184

Query: 193 DSFGYFKAEIQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANKTVQIV 252
           +SFG F  ++  L+LSHNQLSG IP SL   +F  IDLSRNKL GDA ++FGA KT  IV
Sbjct: 185 ESFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIV 244

Query: 253 DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCREIPVG 297
           D+SRN+ +F+LS+V   ++L +LD+NHN + GS   E +K  FQ LNVSYNRLC  IP G
Sbjct: 245 DISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIPKG 304

BLAST of Tan0022676 vs. ExPASy Swiss-Prot
Match: Q9LH52 (Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 9.2e-71
Identity = 147/319 (46.08%), Postives = 189/319 (59.25%), Query Frame = 0

Query: 15  FFLLFFIFTISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCC-DWYSVDCDPTSH 74
           FF + FI T+  +  C  NDK ALL IK A  NP +LSSW+P TDCC  W  V+C  T+ 
Sbjct: 10  FFSILFI-TLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC--TNR 69

Query: 75  RVISLAIVSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHSLKFF------ 134
           RV  L++ SG++SGQI   +GDL  L+TL F  +P+L G I   I KL +L         
Sbjct: 70  RVTGLSVTSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLKHTS 129

Query: 135 -------------------------------SLSQLPNLAALQLDRNKLTGPIPDSFGYF 194
                                          SLSQ+P L A+Q++ NKLTG IP+SFG F
Sbjct: 130 LSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSFGSF 189

Query: 195 KAEIQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANKTVQIVDLSRNL 254
              +  LYLS+N+LSGKIP SLS+ +F  +DLS N   GDAFM FG NKT   VDLSRN+
Sbjct: 190 VGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLSRNM 249

Query: 255 LEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCREIPVGGQLQSF 296
             F+L +V F +S+ SLDL+ N ++G   P LTKL+ ++ NVS N LC +IP GG LQ+F
Sbjct: 250 FNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGLLQTF 309

BLAST of Tan0022676 vs. NCBI nr
Match: XP_008452081.1 (PREDICTED: polygalacturonase inhibitor-like [Cucumis melo] >TYK16665.1 polygalacturonase inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 432.6 bits (1111), Expect = 2.8e-117
Identity = 221/331 (66.77%), Postives = 242/331 (73.11%), Query Frame = 0

Query: 9   KLPWLPFFLLFFIF----TISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDWY 68
           KL WLPFF +FF F    TISFAELCNPNDKK LL IK AFNNPYIL+SW P+TDCC WY
Sbjct: 7   KLSWLPFFFIFFFFFFFITISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWY 66

Query: 69  SVDCDPTSHRVISLAI-VSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHS 128
            V CD T+HR+ISL I    +L+GQIPP+VGDLP LQTLM HK+PNL G IQP IAKLH+
Sbjct: 67  CVKCDRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLHN 126

Query: 129 LKFF-------------------------------------SLSQLPNLAALQLDRNKLT 188
           LKF                                      SLS+LP+L  L+LDRN+LT
Sbjct: 127 LKFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRLDRNQLT 186

Query: 189 GPIPDSFGYFKAE-IQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANK 248
           G IPDSFGYF  E    L LSHNQLSGKIP+SLSR+NFT IDLSRNKL GDAFM FGANK
Sbjct: 187 GQIPDSFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFMFFGANK 246

Query: 249 TVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCR 297
           T+QI+DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFG   P +T+L  QYLNVSYNRLC 
Sbjct: 247 TIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVSYNRLCG 306

BLAST of Tan0022676 vs. NCBI nr
Match: KAA0044799.1 (polygalacturonase inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 432.2 bits (1110), Expect = 3.7e-117
Identity = 221/327 (67.58%), Postives = 241/327 (73.70%), Query Frame = 0

Query: 9   KLPWLPFFLLFFIFTISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDWYSVDC 68
           KL WLPFF  FFI TISFAELCNPNDKK LL IK AFNNPYIL+SW P+TDCC WY V C
Sbjct: 7   KLSWLPFFFFFFI-TISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKC 66

Query: 69  DPTSHRVISLAI-VSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHSLKFF 128
           D T+HR+ISL I    +L+GQIPP+VGDLP LQTLM HK+PNL G IQP IAKLH+LKF 
Sbjct: 67  DRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLHNLKFL 126

Query: 129 -------------------------------------SLSQLPNLAALQLDRNKLTGPIP 188
                                                SLS+LP+L  L+LDRN+LTG IP
Sbjct: 127 DLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRLDRNQLTGQIP 186

Query: 189 DSFGYFKAE-IQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANKTVQI 248
           DSFGYF  E    L LSHNQLSGKIP+SLSR+NFT IDLSRNKL GDAFM FGANKT+QI
Sbjct: 187 DSFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFMFFGANKTIQI 246

Query: 249 VDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCREIPV 297
           +DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFG   P +T+L  QYLNVSYNRLC  IPV
Sbjct: 247 LDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVSYNRLCGPIPV 306

BLAST of Tan0022676 vs. NCBI nr
Match: KAG6595658.1 (Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 426.4 bits (1095), Expect = 2.0e-115
Identity = 217/331 (65.56%), Postives = 240/331 (72.51%), Query Frame = 0

Query: 5   SPNLKLPWLPFFLLFFIFTISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDWY 64
           +PNLKLPWL  FL      IS AELC+ +DKKALLNIK AFNNPYIL+SW P+TDCC WY
Sbjct: 2   APNLKLPWLYIFLFSISIAISVAELCHQDDKKALLNIKKAFNNPYILTSWDPQTDCCHWY 61

Query: 65  SVDCDPTSHRVISLAI-VSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHS 124
            V C+ T+HR+ SL I    +L+GQIPP VGDLP L+TL+ HK+PNL G I P+IAKLH+
Sbjct: 62  CVKCNRTTHRITSLTIFADDRLTGQIPPYVGDLPFLETLILHKLPNLTGPIHPSIAKLHN 121

Query: 125 LKFF-------------------------------------SLSQLPNLAALQLDRNKLT 184
           LK+                                      SLS+LPNL  L+LDRN LT
Sbjct: 122 LKYLDLSWNSLSGPIPDFLSTLSNLFVLSLSFNNFNGTIPNSLSKLPNLGGLELDRNNLT 181

Query: 185 GPIPDSFGYFKAEI-QYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANK 244
           G IPDSFGYF  E    L+LSHNQLSGKIP+SLSR+NFT IDLSRNKL GDAFMIFG NK
Sbjct: 182 GEIPDSFGYFTGEQGPSLFLSHNQLSGKIPSSLSRLNFTSIDLSRNKLKGDAFMIFGLNK 241

Query: 245 TVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCR 297
           TVQIVDLSRN LEFNLSE VFPQSLTSLDLNHNKVFG   PE+TKLNFQYLNVSYNRLC 
Sbjct: 242 TVQIVDLSRNQLEFNLSEAVFPQSLTSLDLNHNKVFGGLPPEMTKLNFQYLNVSYNRLCG 301

BLAST of Tan0022676 vs. NCBI nr
Match: XP_022966388.1 (polygalacturonase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 426.4 bits (1095), Expect = 2.0e-115
Identity = 217/331 (65.56%), Postives = 246/331 (74.32%), Query Frame = 0

Query: 5   SPNLKLPWLPFFLLFFI-FTISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDW 64
           +PNLKL WL F L+FFI   IS AELCNP DKK LL IK AFNNPY+LSSW P+ DCC W
Sbjct: 2   APNLKLSWLLFSLIFFISIAISVAELCNPADKKVLLTIKKAFNNPYVLSSWDPKQDCCTW 61

Query: 65  YSVDCDPTSHRVISLAIVS-GQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLH 124
           YSVDC PT+HRVISL I++  ++SGQIPPDVGDLP LQTLM HK+PNL G IQP IA L 
Sbjct: 62  YSVDCHPTTHRVISLIILADDKISGQIPPDVGDLPFLQTLMLHKLPNLTGPIQPTIANLR 121

Query: 125 SLKFF-------------------------------------SLSQLPNLAALQLDRNKL 184
            LK                                       SLSQLPNL +L LDRNKL
Sbjct: 122 HLKSLDLSWNNLSGSLPSFLGSLKNLTLLNLSFNNLTGTIPSSLSQLPNLGSLHLDRNKL 181

Query: 185 TGPIPDSFGYFKAEIQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANK 244
           TG IPDSFGYF+ EI YLYLSHN+LSGKIPASLS+M+F ++ L+RN+L G A+M+FGANK
Sbjct: 182 TGQIPDSFGYFEGEIPYLYLSHNELSGKIPASLSKMDFNVVSLARNRLEGGAWMLFGANK 241

Query: 245 TVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCR 297
           T QIVDLSRNLLEFNLSEVVFP+SLT+LDLNHNK+FGS    +T+L FQ LNVSYNRLC 
Sbjct: 242 TTQIVDLSRNLLEFNLSEVVFPRSLTTLDLNHNKIFGSIPVAMTELTFQ-LNVSYNRLCG 301

BLAST of Tan0022676 vs. NCBI nr
Match: XP_022151649.1 (polygalacturonase inhibitor-like [Momordica charantia])

HSP 1 Score: 422.2 bits (1084), Expect = 3.8e-114
Identity = 215/334 (64.37%), Postives = 238/334 (71.26%), Query Frame = 0

Query: 3   LPSPNLKLPWLP-FFLLFFIF-TISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDC 62
           + +PNLKLPWLP FF  FF+F T   +ELCNPNDKK LL IK + NNPYIL+SW P+ DC
Sbjct: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60

Query: 63  CDWYSVDCDPTSHRVISLAI-VSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIA 122
           C WY V C PTSHR+ISL I    QLSGQIPP VGDLP L+TLM HK+PNL+G +  A+A
Sbjct: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120

Query: 123 KLHSLKFF-------------------------------------SLSQLPNLAALQLDR 182
            L +LKF                                      SL+ L  L AL LDR
Sbjct: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAVPSSLADLQRLGALHLDR 180

Query: 183 NKLTGPIPDSFGYFKAEIQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFG 242
           NKLTG IP SFGYFK EI  LYLSHNQLSG+IP SL+ M+F +IDLSRNKL GDA MIFG
Sbjct: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240

Query: 243 ANKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNR 297
           ANKT QIVDLSRNLLEFNL+ VVFPQSLTSLDLNHNK+FG   PE+TKL+ QYLNVSYNR
Sbjct: 241 ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300

BLAST of Tan0022676 vs. ExPASy TrEMBL
Match: A0A5D3CYT3 (Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G004960 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.4e-117
Identity = 221/331 (66.77%), Postives = 242/331 (73.11%), Query Frame = 0

Query: 9   KLPWLPFFLLFFIF----TISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDWY 68
           KL WLPFF +FF F    TISFAELCNPNDKK LL IK AFNNPYIL+SW P+TDCC WY
Sbjct: 7   KLSWLPFFFIFFFFFFFITISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWY 66

Query: 69  SVDCDPTSHRVISLAI-VSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHS 128
            V CD T+HR+ISL I    +L+GQIPP+VGDLP LQTLM HK+PNL G IQP IAKLH+
Sbjct: 67  CVKCDRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLHN 126

Query: 129 LKFF-------------------------------------SLSQLPNLAALQLDRNKLT 188
           LKF                                      SLS+LP+L  L+LDRN+LT
Sbjct: 127 LKFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRLDRNQLT 186

Query: 189 GPIPDSFGYFKAE-IQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANK 248
           G IPDSFGYF  E    L LSHNQLSGKIP+SLSR+NFT IDLSRNKL GDAFM FGANK
Sbjct: 187 GQIPDSFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFMFFGANK 246

Query: 249 TVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCR 297
           T+QI+DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFG   P +T+L  QYLNVSYNRLC 
Sbjct: 247 TIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVSYNRLCG 306

BLAST of Tan0022676 vs. ExPASy TrEMBL
Match: A0A1S3BU59 (polygalacturonase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103493193 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.4e-117
Identity = 221/331 (66.77%), Postives = 242/331 (73.11%), Query Frame = 0

Query: 9   KLPWLPFFLLFFIF----TISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDWY 68
           KL WLPFF +FF F    TISFAELCNPNDKK LL IK AFNNPYIL+SW P+TDCC WY
Sbjct: 7   KLSWLPFFFIFFFFFFFITISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWY 66

Query: 69  SVDCDPTSHRVISLAI-VSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHS 128
            V CD T+HR+ISL I    +L+GQIPP+VGDLP LQTLM HK+PNL G IQP IAKLH+
Sbjct: 67  CVKCDRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLHN 126

Query: 129 LKFF-------------------------------------SLSQLPNLAALQLDRNKLT 188
           LKF                                      SLS+LP+L  L+LDRN+LT
Sbjct: 127 LKFLDLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRLDRNQLT 186

Query: 189 GPIPDSFGYFKAE-IQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANK 248
           G IPDSFGYF  E    L LSHNQLSGKIP+SLSR+NFT IDLSRNKL GDAFM FGANK
Sbjct: 187 GQIPDSFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFMFFGANK 246

Query: 249 TVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCR 297
           T+QI+DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFG   P +T+L  QYLNVSYNRLC 
Sbjct: 247 TIQILDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVSYNRLCG 306

BLAST of Tan0022676 vs. ExPASy TrEMBL
Match: A0A5A7TU82 (Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G00900 PE=4 SV=1)

HSP 1 Score: 432.2 bits (1110), Expect = 1.8e-117
Identity = 221/327 (67.58%), Postives = 241/327 (73.70%), Query Frame = 0

Query: 9   KLPWLPFFLLFFIFTISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDWYSVDC 68
           KL WLPFF  FFI TISFAELCNPNDKK LL IK AFNNPYIL+SW P+TDCC WY V C
Sbjct: 7   KLSWLPFFFFFFI-TISFAELCNPNDKKVLLKIKKAFNNPYILTSWDPQTDCCHWYCVKC 66

Query: 69  DPTSHRVISLAI-VSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHSLKFF 128
           D T+HR+ISL I    +L+GQIPP+VGDLP LQTLM HK+PNL G IQP IAKLH+LKF 
Sbjct: 67  DRTTHRIISLTIFADDRLTGQIPPEVGDLPFLQTLMLHKLPNLTGPIQPTIAKLHNLKFL 126

Query: 129 -------------------------------------SLSQLPNLAALQLDRNKLTGPIP 188
                                                SLS+LP+L  L+LDRN+LTG IP
Sbjct: 127 DLSWNGLSGEIPDSLSTLKNLFILTLSFNKLTGKIPSSLSELPSLGGLRLDRNQLTGQIP 186

Query: 189 DSFGYFKAE-IQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANKTVQI 248
           DSFGYF  E    L LSHNQLSGKIP+SLSR+NFT IDLSRNKL GDAFM FGANKT+QI
Sbjct: 187 DSFGYFAGEQAPDLILSHNQLSGKIPSSLSRLNFTSIDLSRNKLEGDAFMFFGANKTIQI 246

Query: 249 VDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCREIPV 297
           +DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFG   P +T+L  QYLNVSYNRLC  IPV
Sbjct: 247 LDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGGLPPVMTELKLQYLNVSYNRLCGPIPV 306

BLAST of Tan0022676 vs. ExPASy TrEMBL
Match: A0A6J1HRZ0 (polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466051 PE=4 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 9.7e-116
Identity = 217/331 (65.56%), Postives = 246/331 (74.32%), Query Frame = 0

Query: 5   SPNLKLPWLPFFLLFFI-FTISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDW 64
           +PNLKL WL F L+FFI   IS AELCNP DKK LL IK AFNNPY+LSSW P+ DCC W
Sbjct: 2   APNLKLSWLLFSLIFFISIAISVAELCNPADKKVLLTIKKAFNNPYVLSSWDPKQDCCTW 61

Query: 65  YSVDCDPTSHRVISLAIVS-GQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLH 124
           YSVDC PT+HRVISL I++  ++SGQIPPDVGDLP LQTLM HK+PNL G IQP IA L 
Sbjct: 62  YSVDCHPTTHRVISLIILADDKISGQIPPDVGDLPFLQTLMLHKLPNLTGPIQPTIANLR 121

Query: 125 SLKFF-------------------------------------SLSQLPNLAALQLDRNKL 184
            LK                                       SLSQLPNL +L LDRNKL
Sbjct: 122 HLKSLDLSWNNLSGSLPSFLGSLKNLTLLNLSFNNLTGTIPSSLSQLPNLGSLHLDRNKL 181

Query: 185 TGPIPDSFGYFKAEIQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANK 244
           TG IPDSFGYF+ EI YLYLSHN+LSGKIPASLS+M+F ++ L+RN+L G A+M+FGANK
Sbjct: 182 TGQIPDSFGYFEGEIPYLYLSHNELSGKIPASLSKMDFNVVSLARNRLEGGAWMLFGANK 241

Query: 245 TVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCR 297
           T QIVDLSRNLLEFNLSEVVFP+SLT+LDLNHNK+FGS    +T+L FQ LNVSYNRLC 
Sbjct: 242 TTQIVDLSRNLLEFNLSEVVFPRSLTTLDLNHNKIFGSIPVAMTELTFQ-LNVSYNRLCG 301

BLAST of Tan0022676 vs. ExPASy TrEMBL
Match: A0A6J1DDN9 (polygalacturonase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111019558 PE=4 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 1.8e-114
Identity = 215/334 (64.37%), Postives = 238/334 (71.26%), Query Frame = 0

Query: 3   LPSPNLKLPWLP-FFLLFFIF-TISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDC 62
           + +PNLKLPWLP FF  FF+F T   +ELCNPNDKK LL IK + NNPYIL+SW P+ DC
Sbjct: 1   MATPNLKLPWLPLFFFFFFLFPTFILSELCNPNDKKVLLKIKQSLNNPYILTSWKPQEDC 60

Query: 63  CDWYSVDCDPTSHRVISLAI-VSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIA 122
           C WY V C PTSHR+ISL I    QLSGQIPP VGDLP L+TLM HK+PNL+G +  A+A
Sbjct: 61  CTWYCVQCHPTSHRIISLTIFADDQLSGQIPPAVGDLPFLETLMLHKLPNLSGTVPAAVA 120

Query: 123 KLHSLKFF-------------------------------------SLSQLPNLAALQLDR 182
            L +LKF                                      SL+ L  L AL LDR
Sbjct: 121 DLRNLKFLDLSRNGLSGPVPEFLGSLANLTFLDLSFNNFTGAVPSSLADLQRLGALHLDR 180

Query: 183 NKLTGPIPDSFGYFKAEIQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFG 242
           NKLTG IP SFGYFK EI  LYLSHNQLSG+IP SL+ M+F +IDLSRNKL GDA MIFG
Sbjct: 181 NKLTGQIPHSFGYFKGEIPDLYLSHNQLSGEIPTSLAGMDFNVIDLSRNKLQGDASMIFG 240

Query: 243 ANKTVQIVDLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNR 297
           ANKT QIVDLSRNLLEFNL+ VVFPQSLTSLDLNHNK+FG   PE+TKL+ QYLNVSYNR
Sbjct: 241 ANKTTQIVDLSRNLLEFNLTAVVFPQSLTSLDLNHNKIFGGIPPEMTKLSLQYLNVSYNR 300

BLAST of Tan0022676 vs. TAIR 10
Match: AT5G06860.1 (polygalacturonase inhibiting protein 1 )

HSP 1 Score: 318.5 bits (815), Expect = 5.5e-87
Identity = 164/320 (51.25%), Postives = 204/320 (63.75%), Query Frame = 0

Query: 16  FLLFFIFTISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDWYSVDC-DPT-SH 75
           FL  F+ T    +LCN NDK  LL IK + NNPY L+SW P+TDCC WY ++C D T +H
Sbjct: 11  FLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDATVNH 70

Query: 76  RVISLAIVSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHSLKFF------ 135
           RV +L I SGQ+SGQIP +VGDLP L+TL+F K+ NL G IQP IAKL +L+        
Sbjct: 71  RVTALTIFSGQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLRLSWTN 130

Query: 136 -------------------------------SLSQLPNLAALQLDRNKLTGPIPDSFGYF 195
                                          SLS LP + AL+L RNKLTG IP+SFG F
Sbjct: 131 LTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPESFGSF 190

Query: 196 KAEIQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANKTVQIVDLSRNL 255
              +  L LSHNQLSG IP SL  ++F  IDLSRNKL GDA M+FG+NKT   +DLSRN+
Sbjct: 191 PGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSIDLSRNM 250

Query: 256 LEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCREIPVGGQLQSF 297
            +F++S+V  P++L  LDLNHN + G+   + T+   Q+ NVSYN+LC  IP GG+LQ+F
Sbjct: 251 FQFDISKVDIPKTLGILDLNHNGITGNIPVQWTEAPLQFFNVSYNKLCGHIPTGGKLQTF 310

BLAST of Tan0022676 vs. TAIR 10
Match: AT5G06870.1 (polygalacturonase inhibiting protein 2 )

HSP 1 Score: 307.8 bits (787), Expect = 9.7e-84
Identity = 165/326 (50.61%), Postives = 204/326 (62.58%), Query Frame = 0

Query: 13  LPFFLLF--FIFTISFA-ELCNPNDKKALLNIKNAFNNPYILSSWHPETDCCDWYSVDC- 72
           +  FLL    + T S A +LC+ +DK  LL IK + NNPY L+SW P+TDCC WY ++C 
Sbjct: 5   MTLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECG 64

Query: 73  DPT-SHRVISLAIVSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHSLKFF 132
           D T +HRV SL I  G++SGQIPP+VGDLP L +L+F K+ NL G IQP IAKL +L F 
Sbjct: 65  DATVNHRVTSLIIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFL 124

Query: 133 -------------------------------------SLSQLPNLAALQLDRNKLTGPIP 192
                                                SLS L  L  L+L RNKLTGPIP
Sbjct: 125 RLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIP 184

Query: 193 DSFGYFKAEIQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANKTVQIV 252
           +SFG F  ++  L+LSHNQLSG IP SL   +F  IDLSRNKL GDA ++FGA KT  IV
Sbjct: 185 ESFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIV 244

Query: 253 DLSRNLLEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCREIPVG 297
           D+SRN+ +F+LS+V   ++L +LD+NHN + GS   E +K  FQ LNVSYNRLC  IP G
Sbjct: 245 DISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSKAYFQLLNVSYNRLCGRIPKG 304

BLAST of Tan0022676 vs. TAIR 10
Match: AT3G12145.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 268.5 bits (685), Expect = 6.5e-72
Identity = 147/319 (46.08%), Postives = 189/319 (59.25%), Query Frame = 0

Query: 15  FFLLFFIFTISFAELCNPNDKKALLNIKNAFNNPYILSSWHPETDCC-DWYSVDCDPTSH 74
           FF + FI T+  +  C  NDK ALL IK A  NP +LSSW+P TDCC  W  V+C  T+ 
Sbjct: 10  FFSILFI-TLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVEC--TNR 69

Query: 75  RVISLAIVSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHSLKFF------ 134
           RV  L++ SG++SGQI   +GDL  L+TL F  +P+L G I   I KL +L         
Sbjct: 70  RVTGLSVTSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLKHTS 129

Query: 135 -------------------------------SLSQLPNLAALQLDRNKLTGPIPDSFGYF 194
                                          SLSQ+P L A+Q++ NKLTG IP+SFG F
Sbjct: 130 LSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSFGSF 189

Query: 195 KAEIQYLYLSHNQLSGKIPASLSRMNFTLIDLSRNKLVGDAFMIFGANKTVQIVDLSRNL 254
              +  LYLS+N+LSGKIP SLS+ +F  +DLS N   GDAFM FG NKT   VDLSRN+
Sbjct: 190 VGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLSRNM 249

Query: 255 LEFNLSEVVFPQSLTSLDLNHNKVFGSFSPELTKLNFQYLNVSYNRLCREIPVGGQLQSF 296
             F+L +V F +S+ SLDL+ N ++G   P LTKL+ ++ NVS N LC +IP GG LQ+F
Sbjct: 250 FNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKLHLEHFNVSDNHLCGKIPSGGLLQTF 309

BLAST of Tan0022676 vs. TAIR 10
Match: AT3G20820.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 129.4 bits (324), Expect = 4.7e-30
Identity = 103/361 (28.53%), Postives = 155/361 (42.94%), Query Frame = 0

Query: 17  LLFFIFTISFAELCNPNDKKALLNIKNAFNNPY--ILSSWHPETDCCDWYSVDCDPTSHR 76
           +L  +  IS A  C P+D++ALL  ++A + PY  I +SW  +  C +WY + CD  +HR
Sbjct: 7   VLLLLLLISSATCCPPSDRRALLAFRSALHEPYLGIFNSWTGQDCCHNWYGISCDSLTHR 66

Query: 77  VI---------------------------------------------------------- 136
           V                                                           
Sbjct: 67  VADINLRGESEDPIFERAHRTGYMTGHISASICELTRLSAITIADWKGISGEIPKCITRL 126

Query: 137 ----SLAIVSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHSLKFFSL--- 196
               +L ++  Q+SG IP D+G L  L  L       ++G I  ++  L SL    L   
Sbjct: 127 PFLRTLDLIGNQISGGIPYDIGRLNRLAVLNVAD-NRISGSIPKSLTNLSSLMHLDLRNN 186

Query: 197 ----------SQLPNLAALQLDRNKLTGPIPDSFG--YFKAEIQYLYLSHNQLSGKIPAS 256
                      +L  L+   L  N++TG IP+S    Y  A++    LS NQL G IP S
Sbjct: 187 LISGVIPSDVGRLKMLSRALLSGNRITGRIPESLTNIYRLADVD---LSGNQLYGTIPPS 246

Query: 257 LSRMN-FTLIDLSRNKLVGDAFMIFGANKTVQIVDLSRNLLEFNLSEVVFPQS-LTSLDL 296
           L RM+    ++L  NK+ G+         +V  ++LSRNLL+  + E   P+S  T LDL
Sbjct: 247 LGRMSVLATLNLDGNKISGEIPQTL-MTSSVMNLNLSRNLLQGKIPEGFGPRSYFTVLDL 306

BLAST of Tan0022676 vs. TAIR 10
Match: AT1G33670.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 113.6 bits (283), Expect = 2.7e-25
Identity = 85/281 (30.25%), Postives = 136/281 (48.40%), Query Frame = 0

Query: 11  PWLPFFLLFFIFTISFAELCNPNDKKALLNIKNAFNN--PYILSSWHPETDCCDWYSVDC 70
           P   F +     + + A  C+P+DK  LL  K+        ILSSW  + DCC WY + C
Sbjct: 7   PLFLFVIFLRCLSSTGAATCHPDDKAGLLAFKSGITQDPSGILSSWQKDIDCCSWYGIFC 66

Query: 71  DPTSH--RVISLAIVSGQLSGQIPPDVGDLPLLQTLMFHKVPNLAGRIQPAIAKLHSLKF 130
            PT H  RV  +A     L G    DVG+              L+G I P +AKLH L  
Sbjct: 67  LPTIHGDRVTMMA-----LDGN--TDVGE------------TFLSGTISPLLAKLHHLNE 126

Query: 131 FSLS--------------QLPNLAALQLDRNKLTGPIPDSFGYFKAEIQYLYLSHNQLSG 190
             L+              +LP L  + L+ N+L+GP+P + G   + ++ L ++ N+ SG
Sbjct: 127 IRLTNLRKITGSFPHFLFKLPKLRTVYLENNRLSGPLPANIGAL-SNLEILSVAGNRFSG 186

Query: 191 KIPASLSRMNFTL-IDLSRNKLVGDAFMIFGANKTVQIVDLSRNLLEFNLSEVV--FPQS 250
            IP+S+S++   L + L+ N+L G    IF + + ++ +DLS N    NL   +     +
Sbjct: 187 SIPSSMSKLTSLLQLKLNGNRLSGIFPDIFKSMRQLRFLDLSSNRFSGNLPSSIASLAPT 246

Query: 251 LTSLDLNHNKVFGSFSPELTKLN-FQYLNVSYNRLCREIPV 270
           L++L++ HNK+ G+    L++      LN+S N     +P+
Sbjct: 247 LSTLEVGHNKLSGTIPDYLSRFELLSALNLSRNGYTGVVPM 267

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q050914.1e-10359.75Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1[more]
A7PW818.3e-9658.25Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1[more]
Q9M5J97.7e-8651.25Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1[more]
Q9M5J81.4e-8250.61Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2[more]
Q9LH529.2e-7146.08Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 ... [more]
Match NameE-valueIdentityDescription
XP_008452081.12.8e-11766.77PREDICTED: polygalacturonase inhibitor-like [Cucumis melo] >TYK16665.1 polygalac... [more]
KAA0044799.13.7e-11767.58polygalacturonase inhibitor-like [Cucumis melo var. makuwa][more]
KAG6595658.12.0e-11565.56Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022966388.12.0e-11565.56polygalacturonase inhibitor-like [Cucurbita maxima][more]
XP_022151649.13.8e-11464.37polygalacturonase inhibitor-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A5D3CYT31.4e-11766.77Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3BU591.4e-11766.77polygalacturonase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103493193 PE=4 SV... [more]
A0A5A7TU821.8e-11767.58Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1HRZ09.7e-11665.56polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466051 PE=... [more]
A0A6J1DDN91.8e-11464.37polygalacturonase inhibitor-like OS=Momordica charantia OX=3673 GN=LOC111019558 ... [more]
Match NameE-valueIdentityDescription
AT5G06860.15.5e-8751.25polygalacturonase inhibiting protein 1 [more]
AT5G06870.19.7e-8450.61polygalacturonase inhibiting protein 2 [more]
AT3G12145.16.5e-7246.08Leucine-rich repeat (LRR) family protein [more]
AT3G20820.14.7e-3028.53Leucine-rich repeat (LRR) family protein [more]
AT1G33670.12.7e-2530.25Leucine-rich repeat (LRR) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 130..296
e-value: 1.7E-41
score: 144.3
coord: 28..129
e-value: 1.7E-20
score: 75.4
IPR013210Leucine-rich repeat-containing N-terminal, plant-typePFAMPF08263LRRNT_2coord: 32..69
e-value: 4.7E-11
score: 42.8
IPR001611Leucine-rich repeatPFAMPF13516LRR_6coord: 228..240
e-value: 0.88
score: 9.8
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 133..193
e-value: 5.0E-7
score: 29.4
NoneNo IPR availablePANTHERPTHR48059:SF10POLYGALACTURONASE INHIBITOR 2coord: 128..295
NoneNo IPR availablePANTHERPTHR48059POLYGALACTURONASE INHIBITOR 1coord: 17..130
coord: 128..295
NoneNo IPR availablePANTHERPTHR48059:SF10POLYGALACTURONASE INHIBITOR 2coord: 17..130
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 29..295

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0022676.1Tan0022676.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding