Tan0022663 (gene) Snake gourd v1

BLAST of Tan0022663 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 3.2e-41
Identity = 87/197 (44.16%), Postives = 115/197 (58.38%), Query Frame = 0

Query: 24  YDYFQMVQQWPPATCS-GVGVTCRSRPPTMFTIHGLWPSNYSNVHLVC-RGLPFNPTQIA 83
           +D F  VQQWPPA CS     +C       FTIHGLWP         C +G PF+ T+I+
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 84  ALTPQLNTYWPNVVSGNNQWFWQHEWDAHGTCSDPPFNQLQYFQTTLNIRTNHNYDLLAI 143
            L  QLNT WPNV+  NNQ FW HEW  HGTCS+  FNQ  YF+  +++R  +NYD++  
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMR--NNYDIIGA 120

Query: 144 LNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLRCNKNAQTGRRQLFEIILCFDKNGVTL 203
           L     GP     +  QAI+G ++A  GK PGLRC  + QT    L +++ CF ++G TL
Sbjct: 121 LRPHAAGPNG-RTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTL 180

Query: 204 IDCTNYAGITCPTHFVW 219
           IDCT     TC  +F++
Sbjct: 181 IDCTR---DTCGANFIF 191

BLAST of Tan0022663 vs. ExPASy Swiss-Prot
Match: O80324 (Ribonuclease S-6 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 2.1e-29
Identity = 75/222 (33.78%), Postives = 113/222 (50.90%), Query Frame = 0

Query: 4   VLLIFSLFSFALVMLSVRAQYDYFQMVQQWPPATCSGVGVTCRSRPPTMFTIHGLWPSN- 63
           V ++FSL    L+  S    Y+YFQ  QQ+ PA C+     C+  P  +FT+HGLWPSN 
Sbjct: 10  VPMVFSL--IVLISCSSTMGYNYFQFTQQYQPAVCNSNPTPCKDPPDKLFTVHGLWPSND 69

Query: 64  YSNVHLVCRGLPFNPTQIAALTPQLNTYWPNVVSGNNQ-WFWQHEWDAHGTCSDPPF--N 123
             +  + C+       QI  LT QL   WPNV+   +   FW  +W+ HG+C   P   +
Sbjct: 70  VGDDPIYCKNKTIKSQQIGNLTAQLIIIWPNVLDRTDHVGFWNRQWNKHGSCGKAPTIKD 129

Query: 124 QLQYFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLRCNKN 183
           ++ YF+T + +      ++  IL+ A + P   + R+   I         KKP L+C KN
Sbjct: 130 EMHYFKTVIKMYITQKQNVSEILSRAKIEPEGKIRRRDDIINAIRLGTKDKKPKLKCQKN 189

Query: 184 AQTGRRQLFEIILCFDKNGVTLIDC--TNYAG--ITCPTHFV 218
            QT   +L EI +C D+N    IDC  +++ G    CPT+ +
Sbjct: 190 NQT--TELVEITICSDRNLTQFIDCPRSSFKGSPFHCPTNHI 227

BLAST of Tan0022663 vs. ExPASy Swiss-Prot
Match: O80325 (Ribonuclease S-7 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 3.1e-28
Identity = 75/205 (36.59%), Postives = 105/205 (51.22%), Query Frame = 0

Query: 4   VLLIFSLFSFALVMLSVRAQYDYFQMVQQWPPATCSGVGVTCRSRPPTMFTIHGLWPSNY 63
           V ++FSL    L++ S    YDYFQ  QQ+ PA C+     C+  P  +FT+HGLWPSN 
Sbjct: 10  VTMVFSL--IVLILSSSTVGYDYFQFTQQYQPAVCNSKPTPCKDPPDKLFTVHGLWPSNL 69

Query: 64  SNVHLV-CRGLPFNPTQIAALTPQLNTYWPNVVSGNNQ-WFWQHEWDAHGTCSDPP-FNQ 123
           +  H   C     NP +I  +  QL   WPNV+   N   FW  +W  HG+C  P   N 
Sbjct: 70  NGPHPENCTNATVNPHRIKNIQAQLKIIWPNVLDRTNHVGFWNKQWIKHGSCGYPAIMND 129

Query: 124 LQYFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAAT-GKKPGLRCNKN 183
             YFQT +N+      ++  IL+ A + P   + R    IE AI+ +T  KKP  +C KN
Sbjct: 130 THYFQTVINMYITQKQNVSEILSKAKIEPLG-IQRPLVHIENAIRNSTNNKKPKFKCQKN 189

Query: 184 AQTGRRQLFEIILCFDKNGVTLIDC 205
             +G  +L E+ LC D +     +C
Sbjct: 190 --SGVTELVEVGLCSDGSLTQFRNC 209

BLAST of Tan0022663 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 124.8 bits (312), Expect = 1.5e-27
Identity = 72/219 (32.88%), Postives = 114/219 (52.05%), Query Frame = 0

Query: 4   VLLIFSLFSFALVMLSVRAQYDYFQMVQQWPPATCSGVGVTC---RSRPPTMFTIHGLWP 63
           + LI  + +  L +L+    +D+F  VQQWP + C      C     +P   F IHGLWP
Sbjct: 9   LFLILLIITQCLSVLNAAKDFDFFYFVQQWPGSYCDTKQSCCYPTTGKPAADFGIHGLWP 68

Query: 64  SNYSNVH--LVCRGLPFNPTQIAALTPQLNTYWPNVV--SGNNQWFWQHEWDAHGTCSDP 123
           +N    +        P++ +QI+ L   +   WP +   SG+   FW HEW+ HGTC++ 
Sbjct: 69  NNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEWEKHGTCAES 128

Query: 124 PF-NQLQYFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLR 183
              NQ  YF+  L+++  +  DLL+IL  A + P          I  AI++A G  P ++
Sbjct: 129 VLTNQHAYFKKALDLK--NQIDLLSILQGADIHPDGESY-DLVNIRNAIKSAIGYTPWIQ 188

Query: 184 CNKNAQTGRRQLFEIILCFDKNGVTLIDCTNYAGITCPT 215
           CN + Q+G  QL+++ +C D +G +LI+C  + G  C T
Sbjct: 189 CNVD-QSGNSQLYQVYICVDGSGSSLIECPIFPGGKCGT 223

BLAST of Tan0022663 vs. ExPASy Swiss-Prot
Match: O80322 (Ribonuclease S-1 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 2.0e-27
Identity = 72/203 (35.47%), Postives = 105/203 (51.72%), Query Frame = 0

Query: 6   LIFSLFSFALVMLSVRAQYDYFQMVQQWPPATCSGVGVTCRSRPPTMFTIHGLWPSNYSN 65
           ++FSL    L++ S    YDYFQ  QQ+ PA C+     C      +FT+HGLWPSN + 
Sbjct: 12  MVFSL--IVLILSSSTVGYDYFQFTQQYQPAVCNSNPTPCNDPTDKLFTVHGLWPSNRNG 71

Query: 66  VH-LVCRGLPFNPTQIAALTPQLNTYWPNVVS-GNNQWFWQHEWDAHGTCSDPPF-NQLQ 125
                C+    N  +I  +T QL   WPNV++  ++  FW+ EW  HGTC  P   + + 
Sbjct: 72  PDPEKCKTTALNSQKIGNMTAQLEIIWPNVLNRSDHVGFWEKEWIKHGTCGYPTIKDDMH 131

Query: 126 YFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGK-KPGLRCNKNAQ 185
           Y QT + +      ++ AIL+ A + P  T  R    IE AI+  T   KP  +C KN +
Sbjct: 132 YLQTVIRMYITQKQNVSAILSKAAIQPNGTN-RPLVDIENAIRRGTNNTKPKFKCQKNTR 191

Query: 186 TGRRQLFEIILCFDKNGVTLIDC 205
           T   +L E+ LC D++    I+C
Sbjct: 192 T-TTELVEVTLCSDRDLKKFINC 210

BLAST of Tan0022663 vs. NCBI nr
Match: XP_038906187.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 468.0 bits (1203), Expect = 5.6e-128
Identity = 223/278 (80.22%), Postives = 243/278 (87.41%), Query Frame = 0

Query: 1   MAKVLLIFSLFSFALVMLSVRAQYDYFQMVQQWPPATCSGVGVTCRSRPPTMFTIHGLWP 60
           MAK LL+ S+FS AL++ SV+AQYDYFQMVQQWPPATCSGVGV CR++PP+MFTIHGLWP
Sbjct: 1   MAKALLVISIFSLALLVFSVKAQYDYFQMVQQWPPATCSGVGVRCRAQPPSMFTIHGLWP 60

Query: 61  SNYSNVHLVCRGLPFNPTQIAALTPQLNTYWPNVVSGNNQWFWQHEWDAHGTCSDPPFNQ 120
           SNYS  HLVC GLPF+PTQIAA+ PQLNTYWP+V+ GNNQ FWQHEWDAHGTCSDPPFNQ
Sbjct: 61  SNYSTAHLVCTGLPFDPTQIAAIRPQLNTYWPDVIRGNNQRFWQHEWDAHGTCSDPPFNQ 120

Query: 121 LQYFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLRCNKNA 180
            QYFQTTLNIR NHNYDLLAILNAAGLGPTATMVRQYQAIE AIQAATGK PGLRCN NA
Sbjct: 121 RQYFQTTLNIRMNHNYDLLAILNAAGLGPTATMVRQYQAIENAIQAATGKIPGLRCNTNA 180

Query: 181 QTGRRQLFEIILCFDKNGVTLIDCTNYAGITCPTHFVWLHQQPLSLASAMGELKDSLVHE 240
           QTG RQLFEIILCFDKNGVTLIDCT +AGITCP  FVWLH+QP SL   + ELKDSLV+E
Sbjct: 181 QTGSRQLFEIILCFDKNGVTLIDCTPFAGITCPLQFVWLHRQPWSLGVVVEELKDSLVYE 240

Query: 241 VSIGMFLFVS-IPLLIAFLVMKRFYQTPKGVGAGGKQE 278
            SI  FLF+S IPL IAF+  K+FYQT +GV  GGKQE
Sbjct: 241 GSIPKFLFLSTIPLSIAFVFWKKFYQTTRGVRGGGKQE 278

BLAST of Tan0022663 vs. NCBI nr
Match: XP_022932791.1 (ribonuclease MC-like [Cucurbita moschata])

HSP 1 Score: 436.0 bits (1120), Expect = 2.4e-118
Identity = 209/277 (75.45%), Postives = 232/277 (83.75%), Query Frame = 0

Query: 1   MAKVLLIFSLFSFALVMLSVRAQYDYFQMVQQWPPATCSGVGVTCRSRPPTMFTIHGLWP 60
           MA+   I S+F F L+   V+AQ+DYFQMVQQWPPATCS  GV C + PP MFTIHGLWP
Sbjct: 1   MAQAPFIVSIFKFVLLTSCVKAQFDYFQMVQQWPPATCS--GVRCYANPPAMFTIHGLWP 60

Query: 61  SNYSNVHLVCRGLPFNPTQIAALTPQLNTYWPNVVSGNNQWFWQHEWDAHGTCSDPPFNQ 120
           SNYSNV LVC G PFNP QIA L PQLNTYWP+V+SGNNQ FW+HEWD+HGTCSDPPFNQ
Sbjct: 61  SNYSNVPLVCAGQPFNPAQIATLVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPFNQ 120

Query: 121 LQYFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLRCNKNA 180
           LQYFQTTLNIRTNHNYDLLAILN AGLGPTAT  RQY AIEGAIQAATGKKPGLRCNKNA
Sbjct: 121 LQYFQTTLNIRTNHNYDLLAILNTAGLGPTATNTRQYGAIEGAIQAATGKKPGLRCNKNA 180

Query: 181 QTGRRQLFEIILCFDKNGVTLIDCTNYAGITCPTHFVWLHQQPLSLASAMGELKDSLVHE 240
           Q+ ++QLFEIILCFDKNGVTLIDCT +AGITCP+ FVWLH+ PLSL SA+GELK+SLVH+
Sbjct: 181 QSKKKQLFEIILCFDKNGVTLIDCTQFAGITCPSQFVWLHRPPLSLVSAVGELKNSLVHQ 240

Query: 241 VSIGMFLFVSIPLLIAFLVMKRFYQTPKGVGAGGKQE 278
           VSI  FLF+ I L +  +  KRFY T +G   GGKQE
Sbjct: 241 VSILKFLFLCILLSVTIMFRKRFYGTRRG---GGKQE 272

BLAST of Tan0022663 vs. NCBI nr
Match: XP_023514499.1 (ribonuclease MC-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 428.7 bits (1101), Expect = 3.8e-116
Identity = 207/277 (74.73%), Postives = 229/277 (82.67%), Query Frame = 0

Query: 1   MAKVLLIFSLFSFALVMLSVRAQYDYFQMVQQWPPATCSGVGVTCRSRPPTMFTIHGLWP 60
           M K   I S+F+ AL+ L V+AQ+DYFQMVQQWPPATCS  GV C + PP MFTIHGLWP
Sbjct: 1   MGKAPFIVSIFNLALLTLCVKAQFDYFQMVQQWPPATCS--GVRCYANPPAMFTIHGLWP 60

Query: 61  SNYSNVHLVCRGLPFNPTQIAALTPQLNTYWPNVVSGNNQWFWQHEWDAHGTCSDPPFNQ 120
           SNYSNV L+C G PFNPT    L PQLNTYWP+V+SGNNQ FW+HEWD+HGTCSDPPFNQ
Sbjct: 61  SNYSNVPLICTGQPFNPT----LVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPFNQ 120

Query: 121 LQYFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLRCNKNA 180
           LQYFQTTLNIRTNHNYDLLAILN AGLGPT T  RQY AIEGAIQAATGKKPGLRCNKNA
Sbjct: 121 LQYFQTTLNIRTNHNYDLLAILNTAGLGPTGTNTRQYGAIEGAIQAATGKKPGLRCNKNA 180

Query: 181 QTGRRQLFEIILCFDKNGVTLIDCTNYAGITCPTHFVWLHQQPLSLASAMGELKDSLVHE 240
           Q+ ++QLFEIILCFDKNGVTLIDCT +AGITCP+ FVWL +QPLSL SA+GELK+SLVH 
Sbjct: 181 QSKKKQLFEIILCFDKNGVTLIDCTQFAGITCPSQFVWLDRQPLSLVSAVGELKNSLVHR 240

Query: 241 VSIGMFLFVSIPLLIAFLVMKRFYQTPKGVGAGGKQE 278
           VSI  FLF+ I L I  +  KRFY T +G   GGKQE
Sbjct: 241 VSILKFLFLCILLSITIMFGKRFYGTRRGGSGGGKQE 271

BLAST of Tan0022663 vs. NCBI nr
Match: XP_022974221.1 (ribonuclease MC-like [Cucurbita maxima])

HSP 1 Score: 423.7 bits (1088), Expect = 1.2e-114
Identity = 204/275 (74.18%), Postives = 226/275 (82.18%), Query Frame = 0

Query: 3   KVLLIFSLFSFALVMLSVRAQYDYFQMVQQWPPATCSGVGVTCRSRPPTMFTIHGLWPSN 62
           K   I S+F+  L+ + V+AQ+DYFQMVQQWPPATCS  GV C + PP MFTIHGLWPS 
Sbjct: 4   KAPFIVSIFNLVLLTIGVKAQFDYFQMVQQWPPATCS--GVRCYANPPAMFTIHGLWPST 63

Query: 63  YSNVHLVCRGLPFNPTQIAALTPQLNTYWPNVVSGNNQWFWQHEWDAHGTCSDPPFNQLQ 122
           YSNV  VC G PF+  QIA L PQLNTYWP+V++GNNQ FW+HEWD HGTCSDPPFNQLQ
Sbjct: 64  YSNVPPVCAGQPFDRAQIATLEPQLNTYWPDVINGNNQRFWKHEWDNHGTCSDPPFNQLQ 123

Query: 123 YFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLRCNKNAQT 182
           YFQTTLNIRTNHNYDLLAILN AGLGPTAT  RQYQAIEGAIQAATGKKPGLRCNKNAQ+
Sbjct: 124 YFQTTLNIRTNHNYDLLAILNTAGLGPTATTTRQYQAIEGAIQAATGKKPGLRCNKNAQS 183

Query: 183 GRRQLFEIILCFDKNGVTLIDCTNYAGITCPTHFVWLHQQPLSLASAMGELKDSLVHEVS 242
            R+QLFEIILCFDKNGVTLI+CT +AGITCP+ FVWL +QPLSL SA+GELK+SLV  VS
Sbjct: 184 KRKQLFEIILCFDKNGVTLINCTQFAGITCPSQFVWLDRQPLSLVSAVGELKNSLVPHVS 243

Query: 243 IGMFLFVSIPLLIAFLVMKRFYQTPKGVGAGGKQE 278
           I  FLF+ I L I  L  KRFY T +G   GGKQE
Sbjct: 244 ILKFLFLCILLSITILFRKRFYGTRRGGSGGGKQE 276

BLAST of Tan0022663 vs. NCBI nr
Match: KAG6581688.1 (hypothetical protein SDJN03_21690, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 407.9 bits (1047), Expect = 6.9e-110
Identity = 192/249 (77.11%), Postives = 210/249 (84.34%), Query Frame = 0

Query: 29  MVQQWPPATCSGVGVTCRSRPPTMFTIHGLWPSNYSNVHLVCRGLPFNPTQIAALTPQLN 88
           MVQQWPPATCS  GV C + P  MFTIHGLWPSNYSN+ LVC G PFN  QIA L PQLN
Sbjct: 1   MVQQWPPATCS--GVRCYANPSAMFTIHGLWPSNYSNIPLVCAGQPFNRAQIATLVPQLN 60

Query: 89  TYWPNVVSGNNQWFWQHEWDAHGTCSDPPFNQLQYFQTTLNIRTNHNYDLLAILNAAGLG 148
           TYWP+V+SGNNQ FW+HEWD+HGTCSDPPFNQLQYFQTTLNIRTNHNYDLLAILN AGLG
Sbjct: 61  TYWPDVISGNNQRFWKHEWDSHGTCSDPPFNQLQYFQTTLNIRTNHNYDLLAILNTAGLG 120

Query: 149 PTATMVRQYQAIEGAIQAATGKKPGLRCNKNAQTGRRQLFEIILCFDKNGVTLIDCTNYA 208
           PT T  RQY AIEGAIQAATGKKPGLRCNKNAQ+ ++QLFEIILCFDKNGVTLIDCT +A
Sbjct: 121 PTGTNTRQYGAIEGAIQAATGKKPGLRCNKNAQSKKKQLFEIILCFDKNGVTLIDCTQFA 180

Query: 209 GITCPTHFVWLHQQPLSLASAMGELKDSLVHEVSIGMFLFVSIPLLIAFLVMKRFYQTPK 268
           GITCP+ FVWL +QPLSL SA+GELK+SLVH+VSI  FLF+ I L I  +  KRFY T +
Sbjct: 181 GITCPSQFVWLDRQPLSLVSAVGELKNSLVHQVSILKFLFLCILLSITIMFRKRFYGTRR 240

Query: 269 GVGAGGKQE 278
           G   GGKQE
Sbjct: 241 GGSGGGKQE 247

BLAST of Tan0022663 vs. ExPASy TrEMBL
Match: A0A6J1EXD5 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111439251 PE=3 SV=1)

HSP 1 Score: 436.0 bits (1120), Expect = 1.1e-118
Identity = 209/277 (75.45%), Postives = 232/277 (83.75%), Query Frame = 0

Query: 1   MAKVLLIFSLFSFALVMLSVRAQYDYFQMVQQWPPATCSGVGVTCRSRPPTMFTIHGLWP 60
           MA+   I S+F F L+   V+AQ+DYFQMVQQWPPATCS  GV C + PP MFTIHGLWP
Sbjct: 1   MAQAPFIVSIFKFVLLTSCVKAQFDYFQMVQQWPPATCS--GVRCYANPPAMFTIHGLWP 60

Query: 61  SNYSNVHLVCRGLPFNPTQIAALTPQLNTYWPNVVSGNNQWFWQHEWDAHGTCSDPPFNQ 120
           SNYSNV LVC G PFNP QIA L PQLNTYWP+V+SGNNQ FW+HEWD+HGTCSDPPFNQ
Sbjct: 61  SNYSNVPLVCAGQPFNPAQIATLVPQLNTYWPDVISGNNQRFWKHEWDSHGTCSDPPFNQ 120

Query: 121 LQYFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLRCNKNA 180
           LQYFQTTLNIRTNHNYDLLAILN AGLGPTAT  RQY AIEGAIQAATGKKPGLRCNKNA
Sbjct: 121 LQYFQTTLNIRTNHNYDLLAILNTAGLGPTATNTRQYGAIEGAIQAATGKKPGLRCNKNA 180

Query: 181 QTGRRQLFEIILCFDKNGVTLIDCTNYAGITCPTHFVWLHQQPLSLASAMGELKDSLVHE 240
           Q+ ++QLFEIILCFDKNGVTLIDCT +AGITCP+ FVWLH+ PLSL SA+GELK+SLVH+
Sbjct: 181 QSKKKQLFEIILCFDKNGVTLIDCTQFAGITCPSQFVWLHRPPLSLVSAVGELKNSLVHQ 240

Query: 241 VSIGMFLFVSIPLLIAFLVMKRFYQTPKGVGAGGKQE 278
           VSI  FLF+ I L +  +  KRFY T +G   GGKQE
Sbjct: 241 VSILKFLFLCILLSVTIMFRKRFYGTRRG---GGKQE 272

BLAST of Tan0022663 vs. ExPASy TrEMBL
Match: A0A6J1IAR7 (Ribonuclease T(2) OS=Cucurbita maxima OX=3661 GN=LOC111472844 PE=3 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 5.9e-115
Identity = 204/275 (74.18%), Postives = 226/275 (82.18%), Query Frame = 0

Query: 3   KVLLIFSLFSFALVMLSVRAQYDYFQMVQQWPPATCSGVGVTCRSRPPTMFTIHGLWPSN 62
           K   I S+F+  L+ + V+AQ+DYFQMVQQWPPATCS  GV C + PP MFTIHGLWPS 
Sbjct: 4   KAPFIVSIFNLVLLTIGVKAQFDYFQMVQQWPPATCS--GVRCYANPPAMFTIHGLWPST 63

Query: 63  YSNVHLVCRGLPFNPTQIAALTPQLNTYWPNVVSGNNQWFWQHEWDAHGTCSDPPFNQLQ 122
           YSNV  VC G PF+  QIA L PQLNTYWP+V++GNNQ FW+HEWD HGTCSDPPFNQLQ
Sbjct: 64  YSNVPPVCAGQPFDRAQIATLEPQLNTYWPDVINGNNQRFWKHEWDNHGTCSDPPFNQLQ 123

Query: 123 YFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLRCNKNAQT 182
           YFQTTLNIRTNHNYDLLAILN AGLGPTAT  RQYQAIEGAIQAATGKKPGLRCNKNAQ+
Sbjct: 124 YFQTTLNIRTNHNYDLLAILNTAGLGPTATTTRQYQAIEGAIQAATGKKPGLRCNKNAQS 183

Query: 183 GRRQLFEIILCFDKNGVTLIDCTNYAGITCPTHFVWLHQQPLSLASAMGELKDSLVHEVS 242
            R+QLFEIILCFDKNGVTLI+CT +AGITCP+ FVWL +QPLSL SA+GELK+SLV  VS
Sbjct: 184 KRKQLFEIILCFDKNGVTLINCTQFAGITCPSQFVWLDRQPLSLVSAVGELKNSLVPHVS 243

Query: 243 IGMFLFVSIPLLIAFLVMKRFYQTPKGVGAGGKQE 278
           I  FLF+ I L I  L  KRFY T +G   GGKQE
Sbjct: 244 ILKFLFLCILLSITILFRKRFYGTRRGGSGGGKQE 276

BLAST of Tan0022663 vs. ExPASy TrEMBL
Match: A0A6J1F837 (Ribonuclease T(2) OS=Cucurbita moschata OX=3662 GN=LOC111441728 PE=3 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 8.0e-96
Identity = 171/236 (72.46%), Postives = 192/236 (81.36%), Query Frame = 0

Query: 1   MAKVLLIFSLFSFALV--MLSVRAQYDYFQMVQQWPPATCSGVGVTCRSRPPTMFTIHGL 60
           MAK   I S+F+F  V  +L V AQ+DYFQMVQQWPPA CS V V C  +PPTMFTIHGL
Sbjct: 1   MAKSPSIVSIFNFVFVVLILCVNAQFDYFQMVQQWPPAKCSSVKVRCHVQPPTMFTIHGL 60

Query: 61  WPSNYSNVHLVCRGLPFNPTQIAALTPQLNTYWPNVVSGNNQWFWQHEWDAHGTCSDPPF 120
           WPSNYSN  LVC   PFNPTQIAAL PQLNTYWP+V+ G NQ FWQHEWD HG CS+PPF
Sbjct: 61  WPSNYSNARLVCAPQPFNPTQIAALRPQLNTYWPDVIQGKNQQFWQHEWDRHGICSNPPF 120

Query: 121 NQLQYFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLRCNK 180
           NQLQYFQ TLNIRTNH+YDLL ILNAAGLGP+ T +R+Y+ IEGAIQAATGKKPGLRCN 
Sbjct: 121 NQLQYFQITLNIRTNHSYDLLTILNAAGLGPSDTNIREYKDIEGAIQAATGKKPGLRCNI 180

Query: 181 NAQT-GRRQLFEIILCFDKNGVTLIDCTNYAGITCPTHFVWLHQQPLSLASAMGEL 234
           NAQ+  ++QL EIILCFDK+G+TLIDCT +AGITCP  FVW  +QPL+L S  GEL
Sbjct: 181 NAQSKEKKQLLEIILCFDKDGITLIDCTEFAGITCPPQFVWPDRQPLNLVSTDGEL 236

BLAST of Tan0022663 vs. ExPASy TrEMBL
Match: A0A6J1E145 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111024988 PE=3 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 3.7e-77
Identity = 160/276 (57.97%), Postives = 188/276 (68.12%), Query Frame = 0

Query: 1   MAKVL-LIFSL-FSFALVMLSVRAQYDYFQMVQQWPPATCSGVGVTCRSRPPTMFTIHGL 60
           MA+ L L+F L F+ +LV       YDYF MVQQW P+TC+   V C ++P  MFTIHGL
Sbjct: 1   MAQTLFLLFKLIFTISLVSSIGVEGYDYFLMVQQWGPSTCA--CVRCYAQPLPMFTIHGL 60

Query: 61  WPSNYSNVH--LVCRGLPFNPTQI-AALTPQLNTYWPNVVSGNNQWFWQHEWDAHGTCSD 120
           WP N ++ H  + C G PF PTQI  A   QL TYWPNV  GN+QWFW +EW  HG CSD
Sbjct: 61  WPGNNNSPHPLINCSGTPFKPTQIPTAQRQQLLTYWPNVEDGNHQWFWGNEWLKHGKCSD 120

Query: 121 PPFNQLQYFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLR 180
           PPFNQ  YFQT LNIRTNHNYDLLAILNAAGLGPT T  R Y AIEGAI AAT + P LR
Sbjct: 121 PPFNQTAYFQTALNIRTNHNYDLLAILNAAGLGPTGTGFRNYTAIEGAIHAATNRTPFLR 180

Query: 181 CNKNAQTGRRQLFEIILCFDKNGVTLIDC----TNYAGITCPTHFVWLHQQPLSLASAMG 240
           C+ NA T R QL+EI+LCFD+NGVTLI+C    T      CP  FVWL + PLSL  A  
Sbjct: 181 CDVNAHTRRYQLYEIVLCFDRNGVTLINCNRNNTRALRKRCPNQFVWLTRNPLSLGVAFW 240

Query: 241 ELKDSLVHEVSIGMFL--FVSIPLLIAFLVMKRFYQ 266
           ELKDSL++ +S+   L  FV++    AF+  KRF+Q
Sbjct: 241 ELKDSLIYHLSLSKLLLTFVALSTAFAFVCWKRFFQ 274

BLAST of Tan0022663 vs. ExPASy TrEMBL
Match: A0A6J1DYQ5 (Ribonuclease T(2) OS=Momordica charantia OX=3673 GN=LOC111024276 PE=3 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 3.0e-66
Identity = 144/280 (51.43%), Postives = 177/280 (63.21%), Query Frame = 0

Query: 1   MAKVLLIFSLFSFALVMLSVRAQYDYFQMVQQWPPATCSGVGVTCRSRPP-TMFTIHGLW 60
           MAK L +  +F F   M +    YDYF MVQQW P  C+GV VTC   PP   FTIHGLW
Sbjct: 1   MAKALFLLLVFVFMFFMGA--KGYDYFLMVQQWGPNKCTGVNVTCHGTPPLPTFTIHGLW 60

Query: 61  P--SNYSNVHLVCRGLPFNPTQI-AALTPQLNTYWPNVVSGNNQWFWQHEWDAHGTCSDP 120
           P  +N     + C G PF+P QI A+   QL+TYWP+V +  NQ FWQHEW+ HG CS P
Sbjct: 61  PGINNSPRPLINCPGPPFDPAQITASQRQQLDTYWPDVQNWRNQSFWQHEWETHGKCSYP 120

Query: 121 PFNQLQYFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLRC 180
            F Q  Y +  L+IR NHNYDLLAI++     PT  M+  Y AIE AIQAAT + P LRC
Sbjct: 121 NFRQTAYLEAALDIRRNHNYDLLAIIH----NPTG-MIHNYNAIEAAIQAATNRTPFLRC 180

Query: 181 NKNAQTGRRQLFEIILCFDKNGVTLIDC----TNYAGITCPTHFVWLHQQPLSLASAMGE 240
           N NA TGR QLFEI+LCFD+NGVTLIDC    T    + CP  FVWL + P SL  A+GE
Sbjct: 181 NVNAHTGRHQLFEIVLCFDRNGVTLIDCNQNNTRALHMQCPHQFVWLPRHPPSLGVAIGE 240

Query: 241 LKDSLVHEVSI-GMFLFVSIPL--LIAFLVMKRFYQTPKG 270
           LKDSL++ +S+  +FL   + L    AF+  +RF++   G
Sbjct: 241 LKDSLIYHLSLPKLFLLTLLALSTAFAFVFRRRFFRAGSG 273

BLAST of Tan0022663 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 122.9 bits (307), Expect = 4.1e-28
Identity = 71/209 (33.97%), Postives = 112/209 (53.59%), Query Frame = 0

Query: 4   VLLIFSLFSFALVMLSVRAQYDYFQMVQQWPPATCSGVGVTC---RSRPPTMFTIHGLWP 63
           ++++ S+FS +    S    +D+F  VQQWP + C      C     +P   F IHGLWP
Sbjct: 14  LVILPSVFSAS----SSSEDFDFFYFVQQWPGSYCDTQKKCCYPNSGKPAADFGIHGLWP 73

Query: 64  SNYSNVHLVCR---GLPFNPTQIAALTPQLNTYWPNVV--SGNNQWFWQHEWDAHGTCSD 123
            NY +           PF+ + I+ L   +   WP +   SG+ + FW+HEW+ HGTCS+
Sbjct: 74  -NYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSE 133

Query: 124 PPFNQLQYFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLR 183
              +Q +YFQT LN++   N  LL  L  AG+ P        ++I  +I+ + G  P + 
Sbjct: 134 SVIDQHEYFQTALNLKQKTN--LLGALTKAGINPDGKSY-SLESIRDSIKESIGFTPWVE 193

Query: 184 CNKNAQTGRRQLFEIILCFDKNGVTLIDC 205
           CN++  +G  QL+++ LC D++G  LI+C
Sbjct: 194 CNRDG-SGNSQLYQVYLCVDRSGSGLIEC 213

BLAST of Tan0022663 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 113.6 bits (283), Expect = 2.5e-25
Identity = 73/219 (33.33%), Postives = 106/219 (48.40%), Query Frame = 0

Query: 4   VLLIFSLFSFALVMLSVRAQYDYFQMVQQWPPATCSGVGVTC---RSRPPTMFTIHGLWP 63
           VLL   +F   L +   +  +D+F  V QWP A C      C     +P   F IHGLWP
Sbjct: 9   VLLKLLVFQ-GLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADFGIHGLWP 68

Query: 64  SNYSNVHLVCRGLP---FNPTQIAALTPQLNTYWPNVVSGNNQW--FWQHEWDAHGTCSD 123
            NY+         P   F+ +QI+ L   L   WP +   +N+   FW+HEW+ HGTCS+
Sbjct: 69  -NYNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSE 128

Query: 124 PPFNQLQYFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLR 183
              +Q +YF+  L ++   N  LL IL  +G+ P          I  AI+   G  PG+ 
Sbjct: 129 SVMDQHEYFENALKLKQKAN--LLQILKNSGINPDDGFY-NLDKITNAIKDGIGFTPGIE 188

Query: 184 CNKNAQTGRRQLFEIILCFDKNGVTLIDCTNYAGITCPT 215
           CNK+ +    QL +I +C D +G   I+C      +CP+
Sbjct: 189 CNKDPER-NAQLHQIYICVDTSGTEFIECPVLPRGSCPS 221

BLAST of Tan0022663 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 106.3 bits (264), Expect = 4.0e-23
Identity = 69/208 (33.17%), Postives = 100/208 (48.08%), Query Frame = 0

Query: 8   FSLFSFALVMLSVRA---QYDYFQMVQQWPPATCSGVGVTC---RSRPPTMFTIHGLWPS 67
           F +F  AL  L V++    +D+F  V QWP A C      C     +P   F IHGLWP 
Sbjct: 3   FFIFILALQQLYVQSFAQDFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWP- 62

Query: 68  NYSNVHLVCRGLP---FNPTQIAALTPQLNTYWPNV--VSGNNQWFWQHEWDAHGTCSDP 127
           NY          P   F+  +++ L   L   WP +   S +   FW HEW+ HGTC++ 
Sbjct: 63  NYKTGGWPQNCNPDSRFDDLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAES 122

Query: 128 PFNQLQYFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLRC 187
             +Q  YF+  L ++   N  LL  L  AG+ P      + + IE  I+   G  PG+ C
Sbjct: 123 ELDQHDYFEAGLKLKQKAN--LLHALTNAGIKPDDKFY-EMKDIENTIKQVVGFAPGIEC 182

Query: 188 NKNAQTGRRQLFEIILCFDKNGVTLIDC 205
           NK++ +   QL++I LC D +    I+C
Sbjct: 183 NKDS-SHNSQLYQIYLCVDTSASKFINC 205

BLAST of Tan0022663 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 103.2 bits (256), Expect = 3.4e-22
Identity = 74/222 (33.33%), Postives = 99/222 (44.59%), Query Frame = 0

Query: 23  QYDYFQMVQQWPPATCSGV------GVTCR-SRPPTMFTIHGLWPS-NYSNVHLVCRGLP 82
           ++DYF +  QWP   C G          CR S  PT FTIHGLWP  N  +    C    
Sbjct: 31  EFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSD 90

Query: 83  FNPTQIAALTPQLNTYWPNV-------VSGNNQWFWQHEWDAHGTCSDPPF-NQLQYFQT 142
           F   +I+ L   L  YWP++        +G    FW HEW+ HGTCS P F ++  YF T
Sbjct: 91  FKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLT 150

Query: 143 TLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQAATGKKPGLRCNKNAQTGRRQ 202
           TLN+   HN  +  +L  AG   + +       I  AIQ A    P + C ++A      
Sbjct: 151 TLNLYLKHN--VTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRDA------ 210

Query: 203 LFEIILCFDKNGVTLIDCTNYAGIT----CPTHFVWLHQQPL 225
           + EI +CF K+     DC     +T    CP +       PL
Sbjct: 211 IDEIRICFYKD-FKPRDCVGSQDLTSRKSCPKYVSLPEYTPL 243

BLAST of Tan0022663 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 99.8 bits (247), Expect = 3.8e-21
Identity = 69/226 (30.53%), Postives = 105/226 (46.46%), Query Frame = 0

Query: 1   MAKVLLIFSLFSFALVMLSVRAQ--YDYFQMVQQWPPATCSGVGVTCRSRPPTM------ 60
           M  ++++  L   +LV+ S + +  +++F  V  WP A C      C   PPT       
Sbjct: 1   MRGIIIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAICDSQKGCC---PPTKGNTASD 60

Query: 61  FTIHGLWPS-NYSNVHLVC-RGLPFNPTQIAALTPQLNTYWPN----VVSGNNQWFWQHE 120
           F IHGLWP  N       C +   F+ ++I+ L  Q+   W          N    W+HE
Sbjct: 61  FIIHGLWPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHE 120

Query: 121 WDAHGTCSDPPFNQLQYFQTTLNIRTNHNYDLLAILNAAGLGPTATMVRQYQAIEGAIQA 180
           W+ HGTC    F+Q  YF+T  N++  H   LL IL   G+ P          I+ AI+ 
Sbjct: 121 WNKHGTCVQSIFDQHSYFRT--NLKFKHKVHLLNILIQKGIKPNDGFY-SLDEIKNAIKC 180

Query: 181 ATGKKPGLRCNKNAQTGRRQLFEIILCFDKNGVTLIDCTNYAGITC 213
           A G  PG+ CN++ + G +QLF+I +C D      ++C      +C
Sbjct: 181 AIGFAPGIECNEDVK-GNKQLFQIYICLDNYAKEFVECPYVPDKSC 219