Tan0022378 (gene) Snake gourd v1

Overview
NameTan0022378
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionubiquitin-conjugating enzyme E2 E2-like
LocationLG06: 74175193 .. 74181086 (-)
RNA-Seq ExpressionTan0022378
SyntenyTan0022378
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAAACGCTTATTCTATTTTTAAGCTCTCAGTTTTTTCTTCACAATTCCGATCGAATCTCTCTATCTCCGCCATGGTTTTTTCCTGTCTCCGCCGTTGCTTCTCTTCAAAATCTTCCTCTTCCACCACCTACGGCCGCATCCACACTCCATCGCCGCCGCGGCCGATTCCGATCTTAGACGAGGATTTTAAACGGCCAGAGGCTGCGCCGCCGCCGCTAATTCCTATCTTCGACGAAGATCTTAAATGGCCGGAGGCTGTGCCACCGCCGGCGATCGCTATCTTCGACGAAGACTTTAAATGGCCGGAGGCTGCGCCGCCGCCGCTAATTCCCATCTTCGACGAGGATTTTAAAAGGCCAGAGGCTGCGCCGCCGCCGGCGATTCCTATCTTCGACGAAGATTTTAAACGGCCAGAGGCTGTGCCGCCGCCGGCGATTCCTGTCTTCGACGAAGATTTTAAACGGGTGGAGGCTGCGCTGCCGCAGCCGATTCCGACCGTAGACGGAGTTAGTGACCAGATAGAGGCTGCGCCGCCGCGCGGTGTTACGATTAGCGACAATGAAGATAAAAAAACTAGTGCGGTGTATAGGATTGAAAACGAGTTGAGAACGATTAACGAGGAGGCGGCGACGCACTGTAGCTTTGGGCCGGTCGGAGCTGACATTTTCCGGTGGGAAGGGGTTGTGGTCGGACCGGCCTTTTCTTGCTATGATGGTGGAATTTTTCAGCTTTCCATTCAATTTCCCTCCGACTACCCCTCGAGTCCTCCCTTCATCAAGTTTCTAACTAAGGTTAGTTATGTCTTCTTTCGTCATTTTACATTTTGATTTTAGTTTTTTTTTCCTGAATTTTTCATTAATTTTAACTCTTTGGGAATTGCATTTTGGAAAGCCTCTCCTATTGTGAAATTGTGATTCTATTATTTTTCTCGATAATATATACATAGTAATTCTATTATTATTACTATTATTTTGTAAAAATCATTTTGGTCTCTCTACTTTTAATAGAATGTCTAAATTTAGTCATGAATCTTAAAATTGTTAGTTTGAAGTTAATTTTTTATTGAAATTTGAATAATAATAATAACTTAGATGCAATAAATGATACAAAATGAATATTTAGAGACTTCTAATAGATACTAAATTTGTTTTAGAAAGATTAGAACATTTGATTTTTTATTTTTTGTTTTAGAAATTTGTGTTTGTTTTCTCACACTTTGTCTACAATGATTTTTATCTTCTCTATCATAGTTTTCATATTTCTTAAGGAAATATTTGAGTTTTGAGTAAAATTAAAAAAATAAATTTTTGAAAACTACCTTTTTTTTTTTTTAGTTTTCAAAACTTGGTTTGGTTTTTGAAAATATAGGTAAAAAGTAGATAAGCATGAAAACTCATGAGTAAAAGTAATATTTATAGATTTAATTTTTAAAAATAATAAATAAAAAAATTAAATAATTATCCAACCGAACCTTAATAATAAATTAACATCAATTTTAAGATTTATTAAAAGTACTAAAATAGAATGAATTGAGATCAGAATGATATATTTTTTTTTTCTTTGAGTAGAAATTGTGATTTTATTATCTATAGTCGTTGTTGTTTAATTATTAGTAGCTTACATTGTGAATTAGGGTTTGGGTCTTACTAGTAGGGCTGTTCTATGTTGGATTGAAGGTATCTTTTAAAAAATTATTATATTATATTATTTTAATTATTTTTGTTGATATAATTGGATTTGTGATTGTCAAGATGGACATAAGTGAATGTTAATTAATATCTATTTAATTCTTTGATGAAGTCGGAAGTTCAAATCCCTAATTTCTATAATTCACACTTATCTTACTAAAAAAAGAAATAATTAAGTTTCATCAAATAAATATGTATTTTTAAATAAAGTAAAAGTTTTGAAATTTTATAAATATATTACAGTCCAATCGAAACCTCAAATTCAAAGTACTATAAACATAATTTAAGAAAACTCACATTTATACTATACTTAGAAAAAAAAAATGAAAAGTTTGGGACACTTTTTTTTTGGCAATCTAGTGGAATTTCTCTTTATGTTTTTATAATGATCATTATTGTTTAATATAATTAACGGTTAACTTCAATTATTCCCCACCTATAATTATTTTTTTAGTATAAAAAAAATGTGAAACTATAGAGGTTTGTAACTCAAATTTTAAAAAGAGTACATTGAACTATACCATTAATAATTGATTTACAAGATTTCATTTTTACCATACTTGTAATTTTTTATTTGTTTTTTTTTTAAACATTTCATCTGAATTTAACTCCTAATCAACTTCTTTTTAAGTTTATATAATATAAATATGAGGTTAGATCATAAATTTAGTCCATGAACTTTTAATTTTATGTCTATTTACCTTAAACTTAAAAAAAAATATAATAAGTTCTTGAATTTTTAATTTTGTGTTTAATAGGTCTCTAAAAAGCCTTTAAACTTTTGATTACGTACTTAGTAGTTTTGCGAATTTTAAAAAATATTGAATACGTCAAAAATCTATTAAACACAAAATTAAAAATTCAAGAACCTATTACATAGAAAATTGAATGTTTAAGAATCTATTATACACTTTCTAAAGTTAGAGACTACATAGACACAAATCTAAAGTTCAAACATCTATTAATCTAAAAATTCAAGGAATTAGTATATTATTAATAAACTATACAAAGTAAACATATCATTATGTTGTTATTTCAACTAATTGAAATATCTCCGTACATTCTACTAATTCTCATTACACTAATAACCTTGATTAATTTTTTAATTATAGTTTTATAACATAATTTTTTGGCCTCTAGAATATATCATCGATCTGGTCCCGTCCCCAAAAATATGTTATTTAGGTATGCTCCCAATTTAGGAATGAATAAAGGTATTCTAAAACCTATTATCAAACAATTTTAAAATAATTATCAAACAAAGTTAGATTATAAGGGATATACGTTTTATTTAAATAAAAGAAATGTTTATAATTAATGATAATACCGACTAGTTAGCTGCAGCCAAAATTATATATAACTTTTGAAGTGAATTGGTCCAACTTATAAGAATGATGTGTTTATATGACTAACATGCAACGTGTACATAAGTCTGGTTGGGAGAGATTTTTGGACCAACCCAAAAATTCAGGTTGGTCATTTCTTCAACCCAACCAACTCTATTCGTGAGAGTAATCTAATCCAACCATCGTTGGGTTAATTTTTTTTTAAATTTTAATTATTTAAAATATTTTTTTTAGATTAATAACTAAAATCACATAAAATTCAAATATCAAACTTCAAAGTTTATAATATCTTAACATTGGTTAAAAGTCTAGAAAAAATAAAAGTTTAGCAAATAATTAATTTATAAGTATATATTTAATTAAAATAAAATTAAAAAAAATGTAAACTTTGGGTTGAGTTGGGTTAACCCAAGCCTAAAAAGACTCTAACTTTTCGATTCTCCAAATTTCTAACCAACTTAAACCGAAAAATAATCTAACCCAATCCAACCCTTAGTTTAGGTTGGATAGTTCTAGTTGCTCAAGTTACCGGATTAGTTGTACACTCCTGTTAGTTTAAAATTTTTTACAAGTCAGTTTTTTTTTTCTCCATCATACCAAGGTACTTCGCATGCAAGAAATGAACTTTGAAATTTGAAAATAAGATGATTGAGTTTAAAATGAAAACTCTAGAATGTTTTTAGGGAGGAAAAATACTCCCTTGTGGGCATCACATGTTTTCCTTTTCTTTTAAAAGAAGGAAAGTCTCTCTTCTCTCTATTTAGTAGTCAAAAAGTCTAGTGATTGAGACTAAGATGTGTGAAGTCTTTCGATGGATGAGGTATGATATTGGGAGTTTGGGATAAAAAGTCTTGAGTTAAGCCTCTTAAGATTATAAGTGTTCAATGTCTTTGAAGCTGACAATAAGATCACGTGGAATCTCTTCATAAGTAAAACTTGATAATACAAATATCAAAAGAAAACAAAGTTTCTGATAAGACACCCCTAATTATCAGTCAAAGATCTAAGTCATGGTTAAGCAAGAACCCTTGAACTAATTAAGAGATTAGCACCTCCATATACAAATTTTGGATTAGCCAAGAGATAAGACTAGAAGTTGATTACCTCTTAAGATCAATGATCTAGAAGTAAGATTGAGTCACTCCACAATCTAGCTTGATCAAGGTTGGATTGAATGATTCGATTGTTTTCTATCACAAAAAAAGCATAGAAGGGTCTCAATCTTGAGAGAACAACTTTTCATTCAATCAAATCATGTCTACAAATGACAAAATATATATATACTAATTGAAAAGACATTTGGAAAGGACACAACCTTTCACCCTTATAGCCTAAATACGTTCCCCACCCCTTCATTTCCATACATGTATTTTCTTAAATGTAAATACATGAATTTAATAGGTACATAATGCATTAGAGATGTGAATAAATTTGGATTTTTAGATTCATTGTGACTTTTGGATGTCAAGCTTGAAGGGTTGCAAGTCTTTACTATGTATTGACACTTTAGCTTGGAGATGGATATTCAATACTTGTTGAAGTCTCTTAACTCTAGCTCTTGCCATTGGGCCTTGTGACATAATGATTGCCTCATGATTGACTTGAATTTTGAGTTGACTCCATTAACTTTTGAATATGAGCTTCATTGGAGTTGGATTAAGCTGGTTGAGGTTTGGATTTTTCTTGTAAACTTCTCTCATGATCTCTTTGCTCATTTATTATCTTATCAGTTTCCACAAACTTGTGTTAAGAGTTCAAGGTTACAAGTTTAAGACATAAAAACCTATGATCACACGATGATACACGAAGTCTTCTTGGGAGAAACTTGATACTATATGTATCAAAATAAAGCACATTCAAGCAGGGGAGTGTTTCCAACCTGTTAAAAATCAAACCTTAAAACTGAAAAGCTCAACTAGCGCACATTTAGTGAGTTTTCTAATGTCTTGCGTGAGCCCCTAAAGTAGGAAGCTCAATAGATGTGTATGTTAATGTCTTTAAGTTGACACTTAGATGTGTGAAATCTCCTTAGAATAAAACACATTCAATGGGAGAGCTATCTCTTGAGTCGAACTTTTGAAGTTAGAAACACAAGAAACATAAATCAGTGATGTTTTCTAGAGCTAAAACTGAGACATACGAAATATTCTTATAAGTAAAAACCATTTGTATCAATGTAAAAAAAACATTCACACACTCCAAACTACTCTTCATCTTAAGACTAAAGATTTTGATTAGTTCAAGAATTTCTTATATGAAAGCAATCACACATTCCAAACTTGAGTTATACCCAATTTTAGTTTGACTTTAGCTCAAAATTTGGGCCATTTCATACTCTTTAAAAAAAGTCCCAAGTTTTGAACTTTCCATGTATTAACAAGTCCATTTTTGTATTCATATTGAAACAGATCTTCCATCCCAATATAGAAGAAGATGGGACCATTCAAATTGACATATTGAAAGAGAATTGGTCTTCAGCATTGACCATTGAAAAACTTATGCTTTCTATATGCTCAATCCTTTCAAACCCAGATCTTCAATACTCCATTAATGAAGCTTCTGAAATGTACCTCAATAATTGGCTCAATTACAATGAGATTGCAAGAGAATGGACCAAAATATATGCAATGGGAAACTGATCTCATGATCACAAAACAGGTACCCTTATTCTATTCTAAATTTTGCACCAAATAACATGTGATGAATGATATCATTCCATTGGTTTAGGGCTCCAAAAGAGGTTGGATTTTGACCTTGGTGATTTTTTTTGTATTATTTTTGCCTAACCCAACATTATATTATATTGTATTTGATTGTATAATGATCTTAGTTTGTATAATTGATTGTATAATTATTTTA

mRNA sequence

GTAAACGCTTATTCTATTTTTAAGCTCTCAGTTTTTTCTTCACAATTCCGATCGAATCTCTCTATCTCCGCCATGGTTTTTTCCTGTCTCCGCCGTTGCTTCTCTTCAAAATCTTCCTCTTCCACCACCTACGGCCGCATCCACACTCCATCGCCGCCGCGGCCGATTCCGATCTTAGACGAGGATTTTAAACGGCCAGAGGCTGCGCCGCCGCCGCTAATTCCTATCTTCGACGAAGATCTTAAATGGCCGGAGGCTGTGCCACCGCCGGCGATCGCTATCTTCGACGAAGACTTTAAATGGCCGGAGGCTGCGCCGCCGCCGCTAATTCCCATCTTCGACGAGGATTTTAAAAGGCCAGAGGCTGCGCCGCCGCCGGCGATTCCTATCTTCGACGAAGATTTTAAACGGCCAGAGGCTGTGCCGCCGCCGGCGATTCCTGTCTTCGACGAAGATTTTAAACGGGTGGAGGCTGCGCTGCCGCAGCCGATTCCGACCGTAGACGGAGTTAGTGACCAGATAGAGGCTGCGCCGCCGCGCGGTGTTACGATTAGCGACAATGAAGATAAAAAAACTAGTGCGGTGTATAGGATTGAAAACGAGTTGAGAACGATTAACGAGGAGGCGGCGACGCACTGTAGCTTTGGGCCGGTCGGAGCTGACATTTTCCGGTGGGAAGGGGTTGTGGTCGGACCGGCCTTTTCTTGCTATGATGGTGGAATTTTTCAGCTTTCCATTCAATTTCCCTCCGACTACCCCTCGAGTCCTCCCTTCATCAAGTTTCTAACTAAGATCTTCCATCCCAATATAGAAGAAGATGGGACCATTCAAATTGACATATTGAAAGAGAATTGGTCTTCAGCATTGACCATTGAAAAACTTATGCTTTCTATATGCTCAATCCTTTCAAACCCAGATCTTCAATACTCCATTAATGAAGCTTCTGAAATGTACCTCAATAATTGGCTCAATTACAATGAGATTGCAAGAGAATGGACCAAAATATATGCAATGGGAAACTGATCTCATGATCACAAAACAGGTACCCTTATTCTATTCTAAATTTTGCACCAAATAACATGTGATGAATGATATCATTCCATTGGTTTAGGGCTCCAAAAGAGGTTGGATTTTGACCTTGGTGATTTTTTTTGTATTATTTTTGCCTAACCCAACATTATATTATATTGTATTTGATTGTATAATGATCTTAGTTTGTATAATTGATTGTATAATTATTTTA

Coding sequence (CDS)

ATGGTTTTTTCCTGTCTCCGCCGTTGCTTCTCTTCAAAATCTTCCTCTTCCACCACCTACGGCCGCATCCACACTCCATCGCCGCCGCGGCCGATTCCGATCTTAGACGAGGATTTTAAACGGCCAGAGGCTGCGCCGCCGCCGCTAATTCCTATCTTCGACGAAGATCTTAAATGGCCGGAGGCTGTGCCACCGCCGGCGATCGCTATCTTCGACGAAGACTTTAAATGGCCGGAGGCTGCGCCGCCGCCGCTAATTCCCATCTTCGACGAGGATTTTAAAAGGCCAGAGGCTGCGCCGCCGCCGGCGATTCCTATCTTCGACGAAGATTTTAAACGGCCAGAGGCTGTGCCGCCGCCGGCGATTCCTGTCTTCGACGAAGATTTTAAACGGGTGGAGGCTGCGCTGCCGCAGCCGATTCCGACCGTAGACGGAGTTAGTGACCAGATAGAGGCTGCGCCGCCGCGCGGTGTTACGATTAGCGACAATGAAGATAAAAAAACTAGTGCGGTGTATAGGATTGAAAACGAGTTGAGAACGATTAACGAGGAGGCGGCGACGCACTGTAGCTTTGGGCCGGTCGGAGCTGACATTTTCCGGTGGGAAGGGGTTGTGGTCGGACCGGCCTTTTCTTGCTATGATGGTGGAATTTTTCAGCTTTCCATTCAATTTCCCTCCGACTACCCCTCGAGTCCTCCCTTCATCAAGTTTCTAACTAAGATCTTCCATCCCAATATAGAAGAAGATGGGACCATTCAAATTGACATATTGAAAGAGAATTGGTCTTCAGCATTGACCATTGAAAAACTTATGCTTTCTATATGCTCAATCCTTTCAAACCCAGATCTTCAATACTCCATTAATGAAGCTTCTGAAATGTACCTCAATAATTGGCTCAATTACAATGAGATTGCAAGAGAATGGACCAAAATATATGCAATGGGAAACTGA

Protein sequence

MVFSCLRRCFSSKSSSSTTYGRIHTPSPPRPIPILDEDFKRPEAAPPPLIPIFDEDLKWPEAVPPPAIAIFDEDFKWPEAAPPPLIPIFDEDFKRPEAAPPPAIPIFDEDFKRPEAVPPPAIPVFDEDFKRVEAALPQPIPTVDGVSDQIEAAPPRGVTISDNEDKKTSAVYRIENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSPPFIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDLQYSINEASEMYLNNWLNYNEIAREWTKIYAMGN
Homology
BLAST of Tan0022378 vs. ExPASy Swiss-Prot
Match: Q2TA10 (Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus OX=9913 GN=UBE2D1 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.3e-35
Identity = 68/146 (46.58%), Postives = 99/146 (67.81%), Query Frame = 0

Query: 170 AVYRIENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYP 229
           A+ RI+ EL  +  +   HCS GPVG D+F W+  ++GP  S Y GG+F L++ FP+DYP
Sbjct: 2   ALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYP 61

Query: 230 SSPPFIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDLQYS-IN 289
             PP I F TKI+HPNI  +G+I +DIL+  WS ALT+ K++LSICS+L +P+     + 
Sbjct: 62  FKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVP 121

Query: 290 EASEMYLNNWLNYNEIAREWTKIYAM 315
           + +++Y ++   YN  AREWT+ YAM
Sbjct: 122 DIAQIYKSDKEKYNRHAREWTQKYAM 147

BLAST of Tan0022378 vs. ExPASy Swiss-Prot
Match: P51668 (Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens OX=9606 GN=UBE2D1 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.3e-35
Identity = 68/146 (46.58%), Postives = 99/146 (67.81%), Query Frame = 0

Query: 170 AVYRIENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYP 229
           A+ RI+ EL  +  +   HCS GPVG D+F W+  ++GP  S Y GG+F L++ FP+DYP
Sbjct: 2   ALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYP 61

Query: 230 SSPPFIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDLQYS-IN 289
             PP I F TKI+HPNI  +G+I +DIL+  WS ALT+ K++LSICS+L +P+     + 
Sbjct: 62  FKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVP 121

Query: 290 EASEMYLNNWLNYNEIAREWTKIYAM 315
           + +++Y ++   YN  AREWT+ YAM
Sbjct: 122 DIAQIYKSDKEKYNRHAREWTQKYAM 147

BLAST of Tan0022378 vs. ExPASy Swiss-Prot
Match: P61080 (Ubiquitin-conjugating enzyme E2 D1 OS=Mus musculus OX=10090 GN=Ube2d1 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.3e-35
Identity = 68/146 (46.58%), Postives = 99/146 (67.81%), Query Frame = 0

Query: 170 AVYRIENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYP 229
           A+ RI+ EL  +  +   HCS GPVG D+F W+  ++GP  S Y GG+F L++ FP+DYP
Sbjct: 2   ALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYP 61

Query: 230 SSPPFIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDLQYS-IN 289
             PP I F TKI+HPNI  +G+I +DIL+  WS ALT+ K++LSICS+L +P+     + 
Sbjct: 62  FKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVP 121

Query: 290 EASEMYLNNWLNYNEIAREWTKIYAM 315
           + +++Y ++   YN  AREWT+ YAM
Sbjct: 122 DIAQIYKSDKEKYNRHAREWTQKYAM 147

BLAST of Tan0022378 vs. ExPASy Swiss-Prot
Match: D3ZDK2 (Ubiquitin-conjugating enzyme E2 D1 OS=Rattus norvegicus OX=10116 GN=Ube2d1 PE=1 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 2.3e-35
Identity = 68/146 (46.58%), Postives = 99/146 (67.81%), Query Frame = 0

Query: 170 AVYRIENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYP 229
           A+ RI+ EL  +  +   HCS GPVG D+F W+  ++GP  S Y GG+F L++ FP+DYP
Sbjct: 2   ALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYP 61

Query: 230 SSPPFIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDLQYS-IN 289
             PP I F TKI+HPNI  +G+I +DIL+  WS ALT+ K++LSICS+L +P+     + 
Sbjct: 62  FKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVP 121

Query: 290 EASEMYLNNWLNYNEIAREWTKIYAM 315
           + +++Y ++   YN  AREWT+ YAM
Sbjct: 122 DIAQIYKSDKEKYNRHAREWTQKYAM 147

BLAST of Tan0022378 vs. ExPASy Swiss-Prot
Match: P25867 (Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophila melanogaster OX=7227 GN=eff PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 3.9e-35
Identity = 69/146 (47.26%), Postives = 98/146 (67.12%), Query Frame = 0

Query: 170 AVYRIENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYP 229
           A+ RI  EL+ +  +    CS GPVG D+F W+  ++GP  S Y GG+F L+I FP+DYP
Sbjct: 2   ALKRINKELQDLGRDPPAQCSAGPVGDDLFHWQATIMGPPDSPYQGGVFFLTIHFPTDYP 61

Query: 230 SSPPFIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDLQYS-IN 289
             PP + F T+I+HPNI  +G+I +DIL+  WS ALTI K++LSICS+L +P+     + 
Sbjct: 62  FKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVP 121

Query: 290 EASEMYLNNWLNYNEIAREWTKIYAM 315
           E + +Y  +   YNE+AREWT+ YAM
Sbjct: 122 EIARIYKTDREKYNELAREWTRKYAM 147

BLAST of Tan0022378 vs. NCBI nr
Match: XP_023000037.1 (ubiquitin-conjugating enzyme E2 E3-like [Cucurbita maxima])

HSP 1 Score: 344.7 bits (883), Expect = 8.2e-91
Identity = 196/324 (60.49%), Postives = 228/324 (70.37%), Query Frame = 0

Query: 1   MVFSCLRRCFSSKSSSSTTYGRIHTPSPPRPIPILDEDFKRPEAAPPPLIPIFDEDLKWP 60
           MV   L  CFS+KSSSST Y R+HTP PP P+PI+  D+ +P+ A               
Sbjct: 1   MVLFRLCCCFSTKSSSSTVYTRLHTPPPP-PLPIIHGDYAQPKPA--------------- 60

Query: 61  EAVPPPAIAIFDEDFKWPEAAPPPLIPIFDEDFKRPEAAPPPAIPIFDEDFKRPEAVPPP 120
           + +P P +   DE FK PEA+PPPL PI   D+  P  A    IPI DE FK+PE   PP
Sbjct: 61  QQLPIPFV---DEGFKQPEASPPPL-PIIHGDYGHPNPAQQLPIPIVDEVFKQPED-SPP 120

Query: 121 AIPVFDEDFKRVEAALPQPIPTVDGVSDQIEAAPPRGVTISDNE-------DKKTSAVYR 180
            +P    DF + + A   PIPT D    Q EA+PPR  T S+ +       ++KTS++YR
Sbjct: 121 RVPNIHGDFGQPKPAQKMPIPTADEGFKQPEASPPRAGTNSEGDGGDGSGNEEKTSSMYR 180

Query: 181 IENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSPP 240
           IENELR +NE AATHCSFGPVGADIFRWEGVV+GPAFSCYDGGIF LSIQFPS+YP SPP
Sbjct: 181 IENELRAMNEVAATHCSFGPVGADIFRWEGVVIGPAFSCYDGGIFHLSIQFPSNYPLSPP 240

Query: 241 FIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDL-QYSINEASE 300
            IKFLTKIFHPN+EEDGTI IDIL +NWS ALTIE LMLSICSILSNP+  + SINEAS 
Sbjct: 241 LIKFLTKIFHPNVEEDGTILIDILMDNWSPALTIETLMLSICSILSNPEPDEGSINEASR 300

Query: 301 MYLNNWLNYNEIAREWTKIYAMGN 317
           M+LNNWLNY +IAREWTK YAMGN
Sbjct: 301 MFLNNWLNYTKIAREWTKKYAMGN 303

BLAST of Tan0022378 vs. NCBI nr
Match: KAG6593782.1 (Ubiquitin-conjugating enzyme E2 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 335.5 bits (859), Expect = 5.0e-88
Identity = 191/324 (58.95%), Postives = 218/324 (67.28%), Query Frame = 0

Query: 1   MVFSCLRRCFSSKSSSSTTYGRIHTPSPPRPIPILDEDFKRPEAAPPPLIPIFDEDLKWP 60
           MV S L  CFS+K SSST Y R HTP PP P+PI+  D+ +P+ A    IPI DE  K P
Sbjct: 1   MVLSRLCCCFSTKFSSSTVYTRFHTPPPP-PVPIIHGDYAQPKPAQQLPIPIVDEGFKQP 60

Query: 61  EAVPPPAIAIFDEDFKWPEAAPPPLIPIFDEDFKRPEAAPPPAIPIFDEDFKRPEAVPPP 120
           E                   A PP +PI   D+  P  A    IPI DE  K+PE   PP
Sbjct: 61  E-------------------ASPPRVPIIHGDYGHPNPAQQLPIPIVDEVSKQPED-SPP 120

Query: 121 AIPVFDEDFKRVEAALPQPIPTVDGVSDQIEAAPPRGVTISDNE-------DKKTSAVYR 180
            +P+   D  +   A   PIPT D    Q EA+PPR    SD +       ++KTSA+YR
Sbjct: 121 RVPIMHGDLGQPMPAQHLPIPTADEGFKQPEASPPRAGGNSDGDGGDGSGNEEKTSAIYR 180

Query: 181 IENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSPP 240
           IENELR +NE AATHCSFGPVGADI RWEG+V+GPAFSCYDGGIF LSIQFPS+YP SPP
Sbjct: 181 IENELRAMNEAAATHCSFGPVGADILRWEGIVIGPAFSCYDGGIFHLSIQFPSNYPLSPP 240

Query: 241 FIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDL-QYSINEASE 300
            IKFLTKIFHPN+EEDGTI IDIL +NWS ALTIE LMLSICSILSNP+  + SINEAS 
Sbjct: 241 LIKFLTKIFHPNVEEDGTILIDILSDNWSPALTIETLMLSICSILSNPEPDEGSINEASR 300

Query: 301 MYLNNWLNYNEIAREWTKIYAMGN 317
           M+LNNWLNY +IAREWTK YAMGN
Sbjct: 301 MFLNNWLNYTKIAREWTKKYAMGN 303

BLAST of Tan0022378 vs. NCBI nr
Match: XP_022964261.1 (ubiquitin-conjugating enzyme E2 E2-like [Cucurbita moschata])

HSP 1 Score: 332.8 bits (852), Expect = 3.2e-87
Identity = 194/317 (61.20%), Postives = 220/317 (69.40%), Query Frame = 0

Query: 1   MVFSCLRRCFSSKSSSSTTYGRIHTPSPPRPIPILDEDFKRPEAAPPPLIPIFDEDLKWP 60
           MV S L  CFS+KSSSST Y R HTP PP P+PI+  D+ +P+ A    IPI DE  K P
Sbjct: 1   MVLSRLCCCFSTKSSSSTVYTRFHTPPPPPPVPIIHGDYAQPKPAQQLPIPIVDEGFKQP 60

Query: 61  EAVPPPAIAIFDEDFKWPEAAPPPLIPIFDEDFKRPEAAPPPAIPIFDEDFKRPEAVPPP 120
           EA  PP + I   D+     A    IPI DE  K+PE + PP +PI   DF +P      
Sbjct: 61  EA-SPPRVPIIHGDYGHLNPAQQLPIPIVDEVSKQPEDS-PPRVPIIHGDFGQPMPAQHL 120

Query: 121 AIPVFDEDFKRVEAALPQPIPTVDGVSDQIEAAPPRGVTISDNEDKKTSAVYRIENELRT 180
            IP  DE FK+ EA+ P+     DG           G   S NE+ KTSA+YRIENELR 
Sbjct: 121 PIPTGDEGFKQPEASPPRAGGNSDG----------DGGDGSGNEE-KTSAMYRIENELRA 180

Query: 181 INEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSPPFIKFLTK 240
           +NE AATHCSFGPVGADI R EG+V+GPAFSCYDGGIF LSIQFPS+YP SPP IKFLTK
Sbjct: 181 MNEAAATHCSFGPVGADILRCEGIVIGPAFSCYDGGIFHLSIQFPSNYPLSPPLIKFLTK 240

Query: 241 IFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDL-QYSINEASEMYLNNWL 300
           IFHPNIEEDGTI IDIL +NWS ALTIE LMLSICSILSNP+  + SINEAS M+LNNWL
Sbjct: 241 IFHPNIEEDGTILIDILTDNWSPALTIETLMLSICSILSNPEPDEGSINEASRMFLNNWL 300

Query: 301 NYNEIAREWTKIYAMGN 317
           NY +IAREWTK YAMGN
Sbjct: 301 NYTKIAREWTKQYAMGN 304

BLAST of Tan0022378 vs. NCBI nr
Match: XP_023513655.1 (ubiquitin-conjugating enzyme E2 E3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 329.3 bits (843), Expect = 3.6e-86
Identity = 193/318 (60.69%), Postives = 222/318 (69.81%), Query Frame = 0

Query: 1   MVFSCLRRCFSSKSSSSTTYGRIHTPSPPRPIPILDEDFKRPEAAPPPLIPIFDEDLKWP 60
           MV S L  CFS+KSSSST Y R+HTP PP P+PI+  D+ +P+ A    IPI DE  + P
Sbjct: 1   MVLSLLCCCFSTKSSSSTVYNRLHTPPPP-PLPIIHGDYAQPKPAHQLPIPIVDEVFEQP 60

Query: 61  EAVPPPAIAIFDEDFKWPEAAPPPLIPIFDEDFKRPEAAPPPAIPIFDEDFKRPEAVPPP 120
           E   PP + I   D+  P       IPI DE  K+PE + PP +PI   DF +P      
Sbjct: 61  ED-SPPRVPIIHGDYGHPNPPQQLPIPIVDEVSKQPEDS-PPRVPIIHGDFGQPMPAQQL 120

Query: 121 AIP-VFDEDFKRVEAALPQPIPTVDGVSDQIEAAPPRGVTISDNEDKKTSAVYRIENELR 180
            IP   DE FK+ EA+ P+P    DG           G   S NE+ KTSA+YRIENELR
Sbjct: 121 PIPTAADEGFKQPEASPPRPGRNSDG----------DGGDGSGNEE-KTSAMYRIENELR 180

Query: 181 TINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSPPFIKFLT 240
            +NE AATHCSFGPVGADI RWEGVV+GPAFSCYDGGIF LSIQFPS+YP SPP IKFLT
Sbjct: 181 AMNEAAATHCSFGPVGADILRWEGVVIGPAFSCYDGGIFHLSIQFPSNYPLSPPLIKFLT 240

Query: 241 KIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDL-QYSINEASEMYLNNW 300
           KIFHPNI+EDGTI IDIL +NWS ALTIE LMLSICSILSNP+  + SINEAS MYL++W
Sbjct: 241 KIFHPNIQEDGTILIDILTDNWSPALTIETLMLSICSILSNPEPDESSINEASRMYLDHW 300

Query: 301 LNYNEIAREWTKIYAMGN 317
           LNY +IAREWTK YAMGN
Sbjct: 301 LNYTKIAREWTKQYAMGN 304

BLAST of Tan0022378 vs. NCBI nr
Match: XP_022138687.1 (ubiquitin-conjugating enzyme E2 5A-like [Momordica charantia] >XP_022138688.1 ubiquitin-conjugating enzyme E2 5A-like [Momordica charantia])

HSP 1 Score: 278.1 bits (710), Expect = 9.4e-71
Identity = 161/316 (50.95%), Postives = 192/316 (60.76%), Query Frame = 0

Query: 1   MVFSCLRRCFSSKSSSSTTYGRIHTPSPPRPIPILDEDFKRPEAAPPPLIPIFDEDLKWP 60
           MV S LRRCFSSKSSSS++Y R+ +P PP P+PI    ++     PPP            
Sbjct: 1   MVLSRLRRCFSSKSSSSSSYSRLRSPPPPPPLPIYHGHYR-----PPP------------ 60

Query: 61  EAVPPPAIAIFDEDFKWPEAAPPPLIPIFDEDFKRPEAAPPPAIPIFDEDFKRPEAVPPP 120
             +PPP                            RP       +PI    +++PE     
Sbjct: 61  --IPPP----------------------------RP-------VPIAAGAYRQPETT--- 120

Query: 121 AIPVFDEDFKRVEAALPQPIPTVDGVSDQIEAAPPRGVTI--SDNEDKKTSAVYRIENEL 180
                                T DG   ++E APPR V+I     +++KTSA+YRIENEL
Sbjct: 121 ---------------------TGDGACGRVEEAPPRRVSIGGGSGDEEKTSAMYRIENEL 180

Query: 181 RTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSPPFIKFL 240
           + +NEE+ATHCSFGPVG DIFRWEG+V+GPA SCY+ GIF LSI  PSDYPS+PP IKF 
Sbjct: 181 KAMNEESATHCSFGPVGGDIFRWEGLVIGPAHSCYEEGIFGLSIDLPSDYPSTPPQIKFQ 238

Query: 241 TKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDLQYSINEASEMYLNNW 300
           TKIFHPNIEEDGTI IDILKENWS ALTIEKL+LSICSILSNP+ Q SINEAS MY N +
Sbjct: 241 TKIFHPNIEEDGTIHIDILKENWSPALTIEKLLLSICSILSNPEAQNSINEASMMYKNEY 238

Query: 301 LNYNEIAREWTKIYAM 315
             + E+AREWTKIYAM
Sbjct: 301 FRFCEVAREWTKIYAM 238

BLAST of Tan0022378 vs. ExPASy TrEMBL
Match: A0A6J1KCF2 (ubiquitin-conjugating enzyme E2 E3-like OS=Cucurbita maxima OX=3661 GN=LOC111494344 PE=4 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 4.0e-91
Identity = 196/324 (60.49%), Postives = 228/324 (70.37%), Query Frame = 0

Query: 1   MVFSCLRRCFSSKSSSSTTYGRIHTPSPPRPIPILDEDFKRPEAAPPPLIPIFDEDLKWP 60
           MV   L  CFS+KSSSST Y R+HTP PP P+PI+  D+ +P+ A               
Sbjct: 1   MVLFRLCCCFSTKSSSSTVYTRLHTPPPP-PLPIIHGDYAQPKPA--------------- 60

Query: 61  EAVPPPAIAIFDEDFKWPEAAPPPLIPIFDEDFKRPEAAPPPAIPIFDEDFKRPEAVPPP 120
           + +P P +   DE FK PEA+PPPL PI   D+  P  A    IPI DE FK+PE   PP
Sbjct: 61  QQLPIPFV---DEGFKQPEASPPPL-PIIHGDYGHPNPAQQLPIPIVDEVFKQPED-SPP 120

Query: 121 AIPVFDEDFKRVEAALPQPIPTVDGVSDQIEAAPPRGVTISDNE-------DKKTSAVYR 180
            +P    DF + + A   PIPT D    Q EA+PPR  T S+ +       ++KTS++YR
Sbjct: 121 RVPNIHGDFGQPKPAQKMPIPTADEGFKQPEASPPRAGTNSEGDGGDGSGNEEKTSSMYR 180

Query: 181 IENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSPP 240
           IENELR +NE AATHCSFGPVGADIFRWEGVV+GPAFSCYDGGIF LSIQFPS+YP SPP
Sbjct: 181 IENELRAMNEVAATHCSFGPVGADIFRWEGVVIGPAFSCYDGGIFHLSIQFPSNYPLSPP 240

Query: 241 FIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDL-QYSINEASE 300
            IKFLTKIFHPN+EEDGTI IDIL +NWS ALTIE LMLSICSILSNP+  + SINEAS 
Sbjct: 241 LIKFLTKIFHPNVEEDGTILIDILMDNWSPALTIETLMLSICSILSNPEPDEGSINEASR 300

Query: 301 MYLNNWLNYNEIAREWTKIYAMGN 317
           M+LNNWLNY +IAREWTK YAMGN
Sbjct: 301 MFLNNWLNYTKIAREWTKKYAMGN 303

BLAST of Tan0022378 vs. ExPASy TrEMBL
Match: A0A6J1HIF1 (ubiquitin-conjugating enzyme E2 E2-like OS=Cucurbita moschata OX=3662 GN=LOC111464331 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 1.6e-87
Identity = 194/317 (61.20%), Postives = 220/317 (69.40%), Query Frame = 0

Query: 1   MVFSCLRRCFSSKSSSSTTYGRIHTPSPPRPIPILDEDFKRPEAAPPPLIPIFDEDLKWP 60
           MV S L  CFS+KSSSST Y R HTP PP P+PI+  D+ +P+ A    IPI DE  K P
Sbjct: 1   MVLSRLCCCFSTKSSSSTVYTRFHTPPPPPPVPIIHGDYAQPKPAQQLPIPIVDEGFKQP 60

Query: 61  EAVPPPAIAIFDEDFKWPEAAPPPLIPIFDEDFKRPEAAPPPAIPIFDEDFKRPEAVPPP 120
           EA  PP + I   D+     A    IPI DE  K+PE + PP +PI   DF +P      
Sbjct: 61  EA-SPPRVPIIHGDYGHLNPAQQLPIPIVDEVSKQPEDS-PPRVPIIHGDFGQPMPAQHL 120

Query: 121 AIPVFDEDFKRVEAALPQPIPTVDGVSDQIEAAPPRGVTISDNEDKKTSAVYRIENELRT 180
            IP  DE FK+ EA+ P+     DG           G   S NE+ KTSA+YRIENELR 
Sbjct: 121 PIPTGDEGFKQPEASPPRAGGNSDG----------DGGDGSGNEE-KTSAMYRIENELRA 180

Query: 181 INEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSPPFIKFLTK 240
           +NE AATHCSFGPVGADI R EG+V+GPAFSCYDGGIF LSIQFPS+YP SPP IKFLTK
Sbjct: 181 MNEAAATHCSFGPVGADILRCEGIVIGPAFSCYDGGIFHLSIQFPSNYPLSPPLIKFLTK 240

Query: 241 IFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDL-QYSINEASEMYLNNWL 300
           IFHPNIEEDGTI IDIL +NWS ALTIE LMLSICSILSNP+  + SINEAS M+LNNWL
Sbjct: 241 IFHPNIEEDGTILIDILTDNWSPALTIETLMLSICSILSNPEPDEGSINEASRMFLNNWL 300

Query: 301 NYNEIAREWTKIYAMGN 317
           NY +IAREWTK YAMGN
Sbjct: 301 NYTKIAREWTKQYAMGN 304

BLAST of Tan0022378 vs. ExPASy TrEMBL
Match: A0A6J1CDR5 (ubiquitin-conjugating enzyme E2 5A-like OS=Momordica charantia OX=3673 GN=LOC111009786 PE=4 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 4.6e-71
Identity = 161/316 (50.95%), Postives = 192/316 (60.76%), Query Frame = 0

Query: 1   MVFSCLRRCFSSKSSSSTTYGRIHTPSPPRPIPILDEDFKRPEAAPPPLIPIFDEDLKWP 60
           MV S LRRCFSSKSSSS++Y R+ +P PP P+PI    ++     PPP            
Sbjct: 1   MVLSRLRRCFSSKSSSSSSYSRLRSPPPPPPLPIYHGHYR-----PPP------------ 60

Query: 61  EAVPPPAIAIFDEDFKWPEAAPPPLIPIFDEDFKRPEAAPPPAIPIFDEDFKRPEAVPPP 120
             +PPP                            RP       +PI    +++PE     
Sbjct: 61  --IPPP----------------------------RP-------VPIAAGAYRQPETT--- 120

Query: 121 AIPVFDEDFKRVEAALPQPIPTVDGVSDQIEAAPPRGVTI--SDNEDKKTSAVYRIENEL 180
                                T DG   ++E APPR V+I     +++KTSA+YRIENEL
Sbjct: 121 ---------------------TGDGACGRVEEAPPRRVSIGGGSGDEEKTSAMYRIENEL 180

Query: 181 RTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSPPFIKFL 240
           + +NEE+ATHCSFGPVG DIFRWEG+V+GPA SCY+ GIF LSI  PSDYPS+PP IKF 
Sbjct: 181 KAMNEESATHCSFGPVGGDIFRWEGLVIGPAHSCYEEGIFGLSIDLPSDYPSTPPQIKFQ 238

Query: 241 TKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDLQYSINEASEMYLNNW 300
           TKIFHPNIEEDGTI IDILKENWS ALTIEKL+LSICSILSNP+ Q SINEAS MY N +
Sbjct: 241 TKIFHPNIEEDGTIHIDILKENWSPALTIEKLLLSICSILSNPEAQNSINEASMMYKNEY 238

Query: 301 LNYNEIAREWTKIYAM 315
             + E+AREWTKIYAM
Sbjct: 301 FRFCEVAREWTKIYAM 238

BLAST of Tan0022378 vs. ExPASy TrEMBL
Match: A0A1S3C6C4 (ubiquitin-conjugating enzyme E2 D1-like OS=Cucumis melo OX=3656 GN=LOC103496968 PE=4 SV=1)

HSP 1 Score: 247.7 bits (631), Expect = 6.6e-62
Identity = 121/184 (65.76%), Postives = 147/184 (79.89%), Query Frame = 0

Query: 137 PQPIPTVDGVS------DQIEAAPPRGVTISDNEDKKTSAVYRIENELRTINEEAATHCS 196
           P P+P +   S      DQ +    RG    +NE+K + A+YRIENEL+ +N+E ATHCS
Sbjct: 23  PPPVPMIKKTSWPCGNIDQKDDDIIRG----NNEEKSSVAMYRIENELKAMNKEVATHCS 82

Query: 197 FGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSPPFIKFLTKIFHPNIEEDG 256
           FGPVG DIFRWEG+V+GPA SCY+GGIF +SIQFPSDYP +PP I FLTKIFHPN+E DG
Sbjct: 83  FGPVGDDIFRWEGIVIGPACSCYEGGIFHVSIQFPSDYPFTPPSIHFLTKIFHPNVELDG 142

Query: 257 TIQIDILKENWSSALTIEKLMLSICSILSNPDLQYSINEASEMYLNNWLNYNEIAREWTK 315
           +I+IDIL ENWSSALTIEKL+LSICS+LSNP  Q S NEAS M+LN+WLN+N+IAR+WTK
Sbjct: 143 SIKIDILYENWSSALTIEKLLLSICSVLSNPIPQGSTNEASTMFLNDWLNFNDIARQWTK 202

BLAST of Tan0022378 vs. ExPASy TrEMBL
Match: A0A0A0LI75 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G138770 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.9e-61
Identity = 113/159 (71.07%), Postives = 137/159 (86.16%), Query Frame = 0

Query: 158 VTISDNEDKKTSAVYRIENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGI 217
           ++  +NE+K + A+YRIENEL+++NEE ATHCSFGPVG DIFRWEGVV+GPA SCY+GGI
Sbjct: 43  ISWGNNEEKSSVAMYRIENELKSMNEEVATHCSFGPVGDDIFRWEGVVIGPAGSCYEGGI 102

Query: 218 FQLSIQFPSDYPSSPPFIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSI 277
           F +SIQFPSDYP +PP I FLTKIFHPN+E DG+I +DIL ENWSSALTIEKL+LSICSI
Sbjct: 103 FHVSIQFPSDYPFTPPSINFLTKIFHPNVEVDGSIGMDILNENWSSALTIEKLLLSICSI 162

Query: 278 LSNPDLQYSINEASEMYLNNWLNYNEIAREWTKIYAMGN 317
           LSNP    S NEAS M+LNNWL++N+ A++WTK+YAM N
Sbjct: 163 LSNPIPHGSTNEASTMFLNNWLDFNDTAKQWTKLYAMPN 201

BLAST of Tan0022378 vs. TAIR 10
Match: AT5G41700.2 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 149.8 bits (377), Expect = 3.6e-36
Identity = 70/144 (48.61%), Postives = 96/144 (66.67%), Query Frame = 0

Query: 173 RIENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSP 232
           RI  EL+ + ++  T CS GPV  D+F W+  ++GPA S Y GG+F ++I FP DYP  P
Sbjct: 5   RILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDYPFKP 64

Query: 233 PFIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDLQYS-INEAS 292
           P + F TK+FHPNI  +G+I +DILKE WS ALTI K++LSICS+L++P+     + E +
Sbjct: 65  PKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIA 124

Query: 293 EMYLNNWLNYNEIAREWTKIYAMG 316
            MY  +   Y   AR WT+ YAMG
Sbjct: 125 HMYKTDRAKYEATARNWTQKYAMG 148

BLAST of Tan0022378 vs. TAIR 10
Match: AT5G41700.1 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 149.8 bits (377), Expect = 3.6e-36
Identity = 70/144 (48.61%), Postives = 96/144 (66.67%), Query Frame = 0

Query: 173 RIENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSP 232
           RI  EL+ + ++  T CS GPV  D+F W+  ++GPA S Y GG+F ++I FP DYP  P
Sbjct: 5   RILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDYPFKP 64

Query: 233 PFIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDLQYS-INEAS 292
           P + F TK+FHPNI  +G+I +DILKE WS ALTI K++LSICS+L++P+     + E +
Sbjct: 65  PKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIA 124

Query: 293 EMYLNNWLNYNEIAREWTKIYAMG 316
            MY  +   Y   AR WT+ YAMG
Sbjct: 125 HMYKTDRAKYEATARNWTQKYAMG 148

BLAST of Tan0022378 vs. TAIR 10
Match: AT5G41700.5 (ubiquitin conjugating enzyme 8 )

HSP 1 Score: 149.8 bits (377), Expect = 3.6e-36
Identity = 70/144 (48.61%), Postives = 96/144 (66.67%), Query Frame = 0

Query: 173 RIENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSP 232
           RI  EL+ + ++  T CS GPV  D+F W+  ++GPA S Y GG+F ++I FP DYP  P
Sbjct: 5   RILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPAESPYSGGVFLVTIHFPPDYPFKP 64

Query: 233 PFIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDLQYS-INEAS 292
           P + F TK+FHPNI  +G+I +DILKE WS ALTI K++LSICS+L++P+     + E +
Sbjct: 65  PKVAFRTKVFHPNINSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIA 124

Query: 293 EMYLNNWLNYNEIAREWTKIYAMG 316
            MY  +   Y   AR WT+ YAMG
Sbjct: 125 HMYKTDRAKYEATARNWTQKYAMG 148

BLAST of Tan0022378 vs. TAIR 10
Match: AT1G64230.1 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 148.7 bits (374), Expect = 8.0e-36
Identity = 68/144 (47.22%), Postives = 96/144 (66.67%), Query Frame = 0

Query: 173 RIENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSP 232
           RI  EL+ + ++  T CS GPV  D+F W+  ++GP+ S Y GG+F ++I FP DYP  P
Sbjct: 5   RILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYPFKP 64

Query: 233 PFIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDLQYS-INEAS 292
           P + F TK+FHPN+  +G+I +DILKE WS ALTI K++LSICS+L++P+     + E +
Sbjct: 65  PKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIA 124

Query: 293 EMYLNNWLNYNEIAREWTKIYAMG 316
            MY  +   Y   AR WT+ YAMG
Sbjct: 125 HMYKTDRAKYESTARSWTQKYAMG 148

BLAST of Tan0022378 vs. TAIR 10
Match: AT1G64230.2 (ubiquitin-conjugating enzyme 28 )

HSP 1 Score: 148.7 bits (374), Expect = 8.0e-36
Identity = 68/144 (47.22%), Postives = 96/144 (66.67%), Query Frame = 0

Query: 173 RIENELRTINEEAATHCSFGPVGADIFRWEGVVVGPAFSCYDGGIFQLSIQFPSDYPSSP 232
           RI  EL+ + ++  T CS GPV  D+F W+  ++GP+ S Y GG+F ++I FP DYP  P
Sbjct: 5   RILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYPFKP 64

Query: 233 PFIKFLTKIFHPNIEEDGTIQIDILKENWSSALTIEKLMLSICSILSNPDLQYS-INEAS 292
           P + F TK+FHPN+  +G+I +DILKE WS ALTI K++LSICS+L++P+     + E +
Sbjct: 65  PKVAFRTKVFHPNVNSNGSICLDILKEQWSPALTISKVLLSICSLLTDPNPDDPLVPEIA 124

Query: 293 EMYLNNWLNYNEIAREWTKIYAMG 316
            MY  +   Y   AR WT+ YAMG
Sbjct: 125 HMYKTDRAKYESTARSWTQKYAMG 148

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q2TA102.3e-3546.58Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus OX=9913 GN=UBE2D1 PE=2 SV=1[more]
P516682.3e-3546.58Ubiquitin-conjugating enzyme E2 D1 OS=Homo sapiens OX=9606 GN=UBE2D1 PE=1 SV=1[more]
P610802.3e-3546.58Ubiquitin-conjugating enzyme E2 D1 OS=Mus musculus OX=10090 GN=Ube2d1 PE=1 SV=1[more]
D3ZDK22.3e-3546.58Ubiquitin-conjugating enzyme E2 D1 OS=Rattus norvegicus OX=10116 GN=Ube2d1 PE=1 ... [more]
P258673.9e-3547.26Ubiquitin-conjugating enzyme E2-17 kDa OS=Drosophila melanogaster OX=7227 GN=eff... [more]
Match NameE-valueIdentityDescription
XP_023000037.18.2e-9160.49ubiquitin-conjugating enzyme E2 E3-like [Cucurbita maxima][more]
KAG6593782.15.0e-8858.95Ubiquitin-conjugating enzyme E2 2, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022964261.13.2e-8761.20ubiquitin-conjugating enzyme E2 E2-like [Cucurbita moschata][more]
XP_023513655.13.6e-8660.69ubiquitin-conjugating enzyme E2 E3-like [Cucurbita pepo subsp. pepo][more]
XP_022138687.19.4e-7150.95ubiquitin-conjugating enzyme E2 5A-like [Momordica charantia] >XP_022138688.1 ub... [more]
Match NameE-valueIdentityDescription
A0A6J1KCF24.0e-9160.49ubiquitin-conjugating enzyme E2 E3-like OS=Cucurbita maxima OX=3661 GN=LOC111494... [more]
A0A6J1HIF11.6e-8761.20ubiquitin-conjugating enzyme E2 E2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1CDR54.6e-7150.95ubiquitin-conjugating enzyme E2 5A-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A1S3C6C46.6e-6265.76ubiquitin-conjugating enzyme E2 D1-like OS=Cucumis melo OX=3656 GN=LOC103496968 ... [more]
A0A0A0LI751.9e-6171.07UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G138770 PE... [more]
Match NameE-valueIdentityDescription
AT5G41700.23.6e-3648.61ubiquitin conjugating enzyme 8 [more]
AT5G41700.13.6e-3648.61ubiquitin conjugating enzyme 8 [more]
AT5G41700.53.6e-3648.61ubiquitin conjugating enzyme 8 [more]
AT1G64230.18.0e-3647.22ubiquitin-conjugating enzyme 28 [more]
AT1G64230.28.0e-3647.22ubiquitin-conjugating enzyme 28 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 172..314
e-value: 3.8E-45
score: 166.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 12..31
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 172..314
NoneNo IPR availablePANTHERPTHR24068:SF242UBIQUITIN CARRIER PROTEINcoord: 172..314
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 160..316
e-value: 6.0E-50
score: 171.0
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 159..314
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 173..308
e-value: 8.6E-37
score: 125.9
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 169..314
score: 42.517815
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 173..309
e-value: 2.05184E-44
score: 146.578

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0022378.1Tan0022378.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0061631 ubiquitin conjugating enzyme activity