Tan0022359 (gene) Snake gourd v1

BLAST of Tan0022359 vs. ExPASy Swiss-Prot
Match: Q9SIT5 (Cation/H(+) antiporter 15 OS=Arabidopsis thaliana OX=3702 GN=CHX15 PE=2 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 1.9e-142
Identity = 292/791 (36.92%), Postives = 466/791 (58.91%), Query Frame = 0

Query: 20  ICTYADRIRCTGVFTGANPLEFSVPLLLLQLGVSAGTIVLFAWLLKPLGQPLIVSQILVG 79
           IC     I   GV+ G NPL+FS+PL +LQL +       F ++LKP  QP ++S+IL G
Sbjct: 14  ICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQPRVISEILGG 73

Query: 80  LVLGSSGLSHLENFRETIFPLRGFICLDAVSALGQIFYFFLIGVQTDISIMKNIDRRAFG 139
           +VLG S L     F  TIFP R  + L+ ++ +G +++ FL+GV+ DI +++   +RA  
Sbjct: 74  IVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMVVRKTGKRALT 133

Query: 140 IGFCSVILPMILITIYPIILVNIVDFKFVKS-LFVLGKIETFINFPMVASLLSELHLINS 199
           I    ++LP ++   +   +    D     + +  LG   +   FP++A +L+EL LIN+
Sbjct: 134 IAIGGMVLPFLIGAAFSFSMHRSEDHLGQGTYILFLGVALSVTAFPVLARILAELKLINT 193

Query: 200 EFGRIALSSSMVSNFLSIFFLLIGAILFPQSVTGSKILSTTYASAVVVVVIFFAIRPIVL 259
           E GRI++S+++V++  +   L +   L     T    L    +SAV + V  F +RP + 
Sbjct: 194 EIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMISSAVFIAVCVFVVRPGIA 253

Query: 260 WMVKKNPIGQQLKEGFVITLLLGVLVVAFCSQASGLHIYFGPLLLGITIPPGPPIGSALV 319
           W+++K P G+   E  +  +L GV++  F + A G H  FG  + G+ IP G P+G  L+
Sbjct: 254 WIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGLVIPNG-PLGLTLI 313

Query: 320 ERLDLITSWVFMPVFFVKIGLVVNIYTIK-LKNFLCISFIILVGALGKFLGALVISLYYK 379
           E+L+   S + +P+FF   GL  NI  I+    +L +  +I +   GK +G ++++ ++ 
Sbjct: 314 EKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAGKVIGTVIVAFFHG 373

Query: 380 LPVRHGVSLGLVLNSQGALELGIFKLMRKNKVINDEAFVVMCICVMVLVAVITPIIGHLF 439
           +PVR G++LGL+LN++G +E+ +  + +  KV++DE F  M +  +V+  VITPI+  L+
Sbjct: 374 MPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALVMTGVITPIVTILY 433

Query: 440 DPSRRYVVHKKRTVMHSRPEIDLCVLVCIHDQEDVPSAINLLEALNPTRRSHLIVYMLHL 499
            P ++ V +K+RT+  ++P+ +L VLVC+H   +VP+ INLLEA +PT+RS + +Y+LHL
Sbjct: 434 KPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRSPICIYVLHL 493

Query: 500 VELLGRANPQLIHHKLTK----IRTSRSCPSEHIVNAFKHFGQSNREIVAIHPFTAISPS 559
           VEL GRA+  LI H   K            S+HI+NAF+++ Q +   VA+ P TAISP 
Sbjct: 494 VELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENYEQ-HAAFVAVQPLTAISPY 553

Query: 560 ATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSKSKMKMVNHHIFDKAPCSVAVVVE 619
           +TM++DVCSLA DKR S I++PFHK+   +G M  +    ++VN ++ + +PCSV ++V+
Sbjct: 554 STMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSVGILVD 613

Query: 620 RGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGARMAGHSNINLTVIRLLEDGNVSS 679
           RG+   TR + +N  S QVA +F GGPDDREA+    RMA H  I LTV+R + D + + 
Sbjct: 614 RGLNGATR-LNSNTVSLQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLRFIHDEDEAD 673

Query: 680 -----------------DDVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDGTGVVSVL 739
                            D  K+R+LD++ +  FR   A    + YIE++V +G   V+ +
Sbjct: 674 TASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVSNGEETVAAV 733

Query: 740 RTMGNNFDLVMVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMILVVQQHT 788
           R+M ++ DL +VGR       L  GL  W+E  ELGAIG++LASSDF     +LVVQQ+ 
Sbjct: 734 RSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAATVSVLVVQQYV 793

BLAST of Tan0022359 vs. ExPASy Swiss-Prot
Match: Q9LMJ1 (Cation/H(+) antiporter 14 OS=Arabidopsis thaliana OX=3702 GN=CHX14 PE=2 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 1.4e-113
Identity = 251/785 (31.97%), Postives = 442/785 (56.31%), Query Frame = 0

Query: 20  ICTYADRIRCTGVFTGANPLEFSVPLLLLQLGVSAGTIVLFAWLLKPLGQPLIVSQILVG 79
           +C     +   GVF G++PL++++PL+LLQ+ V   T  L   LLKPL Q +I +Q+L G
Sbjct: 27  VCQKNHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLAG 86

Query: 80  LVLGSSGLSHLENFRETIFPLRGFICLDAVSALGQIFYFFLIGVQTDISIMKNIDRRAFG 139
           ++LG S       + +   P+ G I L  +S LG   + FL+G++ D SI++    +A  
Sbjct: 87  IILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAIL 146

Query: 140 IGFCSVILPMILITIYPIILVNIVDF--KFVKSLFVLGKIETFINFPMVASLLSELHLIN 199
           IG  S  LP  L  +  + L N  +     V  +  +  +    +FP+  ++L+EL+++N
Sbjct: 147 IGTASYALPFSLGNLTVLFLKNTYNLPPDVVHCISTVISLNAMTSFPVTTTVLAELNILN 206

Query: 200 SEFGRIALSSSMVSNFLSIFFLLIGAILFPQSVTGSKILSTTYASAVVVVVIFFAIRPIV 259
           S+ GR+A + S+V    S    L+   +F +  T + + S  + +A +++VIFF  RP +
Sbjct: 207 SDLGRLATNCSIVCEAFSWIVALVFR-MFLRDGTLASVWSFVWVTA-LILVIFFVCRPAI 266

Query: 260 LWMVKKNPIG-QQLKEGFVITLLLGVLVVAFCSQASGLHIYFGPLLLGITIPPGPPIGSA 319
           +W+ ++  I   +  E     +++ +L ++  S+  G+H  FG   LG+++P GPP+G+ 
Sbjct: 267 IWLTERRSISIDKAGEIPFFPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPLGTG 326

Query: 320 LVERLDLITSWVFMPVFFVKIGLVVNIYTIKLKNFLCISFIILVGALGKFLGALVISLYY 379
           L  +L++  + + +P F    GL  N + I   +   I  +IL+    KFLG    S Y 
Sbjct: 327 LTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFLGTAAASAYC 386

Query: 380 KLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVINDEAFVVMCICVMVLVAVITPIIGHL 439
            + +    SL L++  QG +E+    + +  KV+N E F ++ I ++++  +   ++  L
Sbjct: 387 NIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRFLVVCL 446

Query: 440 FDPSRRYVVHKKRTVMHSRP-EIDLCVLVCIHDQEDVPSAINLLEALNPTRRSHLIVYML 499
           +DPS+RY    KRT++ +R   +   +L+C+++ E+VPS +NLLEA  P+R S + V+ L
Sbjct: 447 YDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPISVFTL 506

Query: 500 HLVELLGRANPQLI-HHKLTKIRTSRSCPSEHIVNAFKHFGQSNREIVAIHPFTAISPSA 559
           HLVEL GRA+  L+ HH++ K+    +  S HIVN F+ F Q N+  +    FTA +P +
Sbjct: 507 HLVELKGRAHAVLVPHHQMNKL-DPNTVQSTHIVNGFQRFEQQNQGTLMAQHFTAAAPFS 566

Query: 560 TMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSKSKMKMVNHHIFDKAPCSVAVVVER 619
           ++ DD+C+LALDK+++LI++PFHK++  +G +      ++ +N ++ +KAPCSV + ++R
Sbjct: 567 SINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGIFIDR 626

Query: 620 GIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGARMAGHSNINLTVIRLLEDGNVSSD 679
           G     RS+  +     VA +F+ G DD EA+    R+A H  +++T+I      ++  +
Sbjct: 627 GETEGRRSVLMSYTWRNVAVIFIEGRDDAEALAFSMRIAEHPEVSVTMIHFRHKSSLQQN 686

Query: 680 ---DVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDGTGVVSVLRTMGNNFDLVMVGRR 739
              DV+    ++  + +F+    +  K+ Y EE+V+DG     V+ ++G++FDLV+VGR 
Sbjct: 687 HVVDVESELAESYLINDFKNFAMSKPKISYREEIVRDGVETTQVISSLGDSFDLVVVGRD 746

Query: 740 HSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMILVVQQHTKVVNEEEENHFGTNI 797
           H    +++ GL  W+E  ELG IG++ ASSDF  +  +LV+ Q             G ++
Sbjct: 747 HDLESSVLYGLTDWSECPELGVIGDMFASSDF--HFSVLVIHQQE-----------GDSL 795

BLAST of Tan0022359 vs. ExPASy Swiss-Prot
Match: O22920 (Cation/H(+) symporter 13 OS=Arabidopsis thaliana OX=3702 GN=CHX13 PE=1 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 5.1e-108
Identity = 245/768 (31.90%), Postives = 431/768 (56.12%), Query Frame = 0

Query: 20  ICTYADRIRCTGVFTGANPLEFSVPLLLLQLGVSAGTIVLFAWLLKPLGQPLIVSQILVG 79
           +C   + +   G+F  +NPL++++PLLLLQ+ V   T  L   +L+PL Q +I +Q+L G
Sbjct: 29  VCQAQNMLTSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLTG 88

Query: 80  LVLGSSGLSHLENFRETIFPLRGFICLDAVSALGQIFYFFLIGVQTDISIMKNIDRRAFG 139
           +VLG S L H   +     P  G I +  +S +G + + FL+G++ D SI++    +A  
Sbjct: 89  VVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAIL 148

Query: 140 IGFCSVILPMIL--ITIYPIILVNIVDFKFVKSLFVLGKIETFINFPMVASLLSELHLIN 199
           IG  S   P  L  +TI  I     +    +        + +  +FP+  ++L+EL+++N
Sbjct: 149 IGTASYAFPFSLGNLTIMFISKTMGLPSDVISCTSSAISLSSMTSFPVTTTVLAELNILN 208

Query: 200 SEFGRIALSSSMVSNFLSIFFLLIGAILFPQSVTGSKILSTTYASAVVV---VVIFFAIR 259
           SE GR+A   SMV    S F  L   +      T  + +++ YA ++++   +VI+F  R
Sbjct: 209 SELGRLATHCSMVCEVCSWFVALAFNL-----YTRDRTMTSLYALSMIIGLLLVIYFVFR 268

Query: 260 PIVLWMVKKNPIGQQLKE---GFVITLLLGVLVVAFCSQASGLHIYFGPLLLGITIPPGP 319
           PI++W+ ++       K+    F + LLL   + +   +A G+H  FG   LG+++P GP
Sbjct: 269 PIIVWLTQRKTKSMDKKDVVPFFPVLLLLS--IASLSGEAMGVHAAFGAFWLGVSLPDGP 328

Query: 320 PIGSALVERLDLITSWVFMPVFFVKIGLVVNIYTIKLK---NFLCISFIILVGALGKFLG 379
           P+G+ L  +L++  S +F+P F    GL  N + I      + + I  I+L+    KFLG
Sbjct: 329 PLGTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCKFLG 388

Query: 380 ALVISLYYKLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVINDEAFVVMCICVMVLVAV 439
               S Y +  +   + L  ++  QG +E+    + +  +V++ E F ++ I ++ +  +
Sbjct: 389 TAAASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQVVDTECFNLVIITILFVTGI 448

Query: 440 ITPIIGHLFDPSRRYVVHKKRTVMHSRP-EIDLCVLVCIHDQEDVPSAINLLEALNPTRR 499
              ++ +L+DPS+RY    KRT++++R   + L +L+ +++ E+VPS +NLLEA  PTR 
Sbjct: 449 SRFLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYNVENVPSMVNLLEATYPTRF 508

Query: 500 SHLIVYMLHLVELLGRANPQLI-HHKLTKIRTSRSCPSEHIVNAFKHFGQSNREIVAIHP 559
           + +  + LHLVEL GRA+  L  HH++ K+    +  S HIVNAF+ F Q  +  +    
Sbjct: 509 NPISFFTLHLVELKGRAHALLTPHHQMNKL-DPNTAQSTHIVNAFQRFEQKYQGALMAQH 568

Query: 560 FTAISPSATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSKSKMKMVNHHIFDKAPC 619
           FTA +P +++ +D+C+LALDK+++LI++PFHK++  +G +      ++ +N ++ D APC
Sbjct: 569 FTAAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNGPIRTINLNVLDAAPC 628

Query: 620 SVAVVVERGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGARMAGHSNINLTVIRLL 679
           SVA+ ++RG     RS+        VA +F+GG DD EA+ +  RMA   ++N+T+I   
Sbjct: 629 SVAIFIDRGETEGRRSVLMTNTWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMIHFR 688

Query: 680 EDGNVSSDDVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDGTGVVSVLRTMGNNFDLV 739
               +  +D  +   +   + +F+   AN  K+ Y+EE+V+DG     V+ ++G+ +D+V
Sbjct: 689 HKSALQDEDYSDMS-EYNLISDFKSYAANKGKIHYVEEIVRDGVETTQVISSLGDAYDMV 748

Query: 740 MVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMILVVQQ 775
           +VGR H    +++ GL  W+E  ELG IG++L S DF  + +++  QQ
Sbjct: 749 LVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDFHFSVLVVHQQQ 787

BLAST of Tan0022359 vs. ExPASy Swiss-Prot
Match: Q9FFR9 (Cation/H(+) antiporter 18 OS=Arabidopsis thaliana OX=3702 GN=CHX18 PE=2 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 5.1e-100
Identity = 242/789 (30.67%), Postives = 423/789 (53.61%), Query Frame = 0

Query: 16  NFTSICTYADRIRCTGVFTGANPLEFSVPLLLLQLGVSAGTIVLFAWLLKPLGQPLIVSQ 75
           N T  C    +    GVF G NP++F++PL +LQ+ +      + A+LL+PL QP ++++
Sbjct: 4   NSTKACPAPMKATSNGVFQGDNPIDFALPLAILQIVIVIVLTRVLAYLLRPLRQPRVIAE 63

Query: 76  ILVGLVLGSSGLSHLENFRETIFPLRGFICLDAVSALGQIFYFFLIGVQTDISIMKNIDR 135
           ++ G++LG S L   + F + +FP +    L+ ++ LG +F+ FL G++ D   ++   +
Sbjct: 64  VIGGIMLGPSLLGRSKAFLDAVFPKKSLTVLETLANLGLLFFLFLAGLEIDTKALRRTGK 123

Query: 136 RAFGIGFCSVILPMILITIYPIILVNIVDFKFVKS---LFVLGKIETFINFPMVASLLSE 195
           +A GI    + LP  L      +L   +  K V S   L  +G   +   FP++A +L+E
Sbjct: 124 KALGIALAGITLPFALGIGSSFVLKATIS-KGVNSTAFLVFMGVALSITAFPVLARILAE 183

Query: 196 LHLINSEFGRIALSSSMVSNFLSIFFLLIGAILFPQSVTGSKILSTTYASAVVVVV-IFF 255
           L L+ +E GR+A+S++ V N ++ + LL  AI    S T   +    + S    V+   F
Sbjct: 184 LKLLTTEIGRLAMSAAAV-NDVAAWILLALAIALSGSNTSPLVSLWVFLSGCAFVIGASF 243

Query: 256 AIRPIVLWMVKKNPIGQQLKEGFVITLLLGVLVVAFCSQASGLHIYFGPLLLGITIPPGP 315
            I PI  W+ ++   G+ ++E ++   L  VLV  F + A G+H  FG  ++G+ IP   
Sbjct: 244 IIPPIFRWISRRCHEGEPIEETYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPKEG 303

Query: 316 PIGSALVERLDLITSWVFMPVFFVKIGLVVNIYTIK-LKNFLCISFIILVGALGKFLGAL 375
           P   ALVE+++ + S +F+P++FV  GL  N+ TI+  +++  +  +      GK LG L
Sbjct: 304 PFAGALVEKVEDLVSGLFLPLYFVASGLKTNVATIQGAQSWGLLVLVTATACFGKILGTL 363

Query: 376 VISLYYKLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVINDEAFVVMCICVMVLVAVIT 435
            +SL +K+P+R  ++LG ++N++G +EL +  + +  KV+ND+ F +M +  +    + T
Sbjct: 364 GVSLAFKIPMREAITLGFLMNTKGLVELIVLNIGKDRKVLNDQTFAIMVLMALFTTFITT 423

Query: 436 PIIGHLFDPSRRYV---VHKKRTVMHSRPEIDLCVLVCIHDQEDVPSAINLLEALNPTRR 495
           P++  ++ P+RR      +K R V        L +L C H    +PS INLLEA     +
Sbjct: 424 PVVMAVYKPARRAKKEGEYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGIEK 483

Query: 496 SH-LIVYMLHLVELLGRANPQLIHHKLTK------IRTSRSCPSEHIVNAFKHFGQSNRE 555
              L VY LHL EL  R++  L+ HK+ K       R   +  ++ +V AF+ F Q +R 
Sbjct: 484 GEGLCVYALHLRELSERSSAILMVHKVRKNGMPFWNRRGVNADADQVVVAFQAFQQLSR- 543

Query: 556 IVAIHPFTAISPSATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSKSKMKMVNHHI 615
            V + P TAIS  + +++D+C+ A+ K+++++++PFHK    +G +  ++   + VN  +
Sbjct: 544 -VNVRPMTAISSMSDIHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRV 603

Query: 616 FDKAPCSVAVVVERGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGARMAGHSNINL 675
             +APCSV + V+RG+   ++  A ++ S+ V  +F GGPDDREA+  G RMA H  I L
Sbjct: 604 LLQAPCSVGIFVDRGLGGSSQVSAQDV-SYSVVVLFFGGPDDREALAYGLRMAEHPGIVL 663

Query: 676 TVIRL--------------LEDGNVSSDDVKERRLDNEAVVEFRQMMANNYKVRYIEEVV 735
           TV R               + + N  +  VK  + D E + E R++ + +  V+++E+ +
Sbjct: 664 TVFRFVVSPERVGEIVNVEVSNNNNENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQI 723

Query: 736 KDGTGVVSVLRTMGNNFDLVMVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGN 776
           ++    V          +L +VGR     +AL   +   +E  ELG +G +L S +    
Sbjct: 724 ENAAVDVRSAIEEVRRSNLFLVGRMPGGEIAL--AIRENSECPELGPVGSLLISPESSTK 783

BLAST of Tan0022359 vs. ExPASy Swiss-Prot
Match: Q9FFB8 (Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 4.8e-98
Identity = 239/772 (30.96%), Postives = 415/772 (53.76%), Query Frame = 0

Query: 57  IVLFAW-----LLKPLGQPLIVSQILVGLVLGSSGLSH---------LENFRETIFPLRG 116
           I+ F W      L+ LG     S +L G++L  S L            E+++E +F L  
Sbjct: 64  IISFLWQFLHFFLRRLGMIRFTSHMLTGVLLSKSFLKENSAARRFFSTEDYKEIVFSL-- 123

Query: 117 FICLDAVSALGQIFYFFLIGVQTDISIMKNIDRRAFGIGFCSVILPMILITIYPIILVNI 176
                  +A   + ++FL+GV+ D  +++   R+A  IG  SV+L  ++ ++  I   N+
Sbjct: 124 ------TAACSYMMFWFLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSV--IFFGNL 183

Query: 177 VDFKFVKSLFVLGKIETFI--------NFPMVASLLSELHLINSEFGRIALSSSMVSNF- 236
            D     S   L  +E  +        +FP+V +LL EL L NSE GR+A+SS+++S+F 
Sbjct: 184 RDVGTKNSDHTLNSLEYVVIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFS 243

Query: 237 ----LSIFFLL---------IGAILFPQSVTGSKILSTTYASAVVVVVIFFAIRPIVLWM 296
                S+   +         +G++     + G++ L       + V +  +  RP++ ++
Sbjct: 244 TSILASVLIFMKELKDEQTRLGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYI 303

Query: 297 VKKNPIGQQLKEGFVITLLLGVLVVAFCSQASGLHIYFGPLLLGITIPPGPPIGSALVER 356
           +K+ P G+ +K  ++ T+++ V   A  +      I+ GP +LG+ +P GPP+GSA++++
Sbjct: 304 IKQTPSGRPVKAIYLSTIIVMVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQK 363

Query: 357 LDLITSWVFMPVFFVKIGLVVNIYTI-KLKNFLCISFIILVGALGKFLGALVISLYYKLP 416
            +      F+P F       ++I  +   +    I  I++   + KF+   V +L+Y +P
Sbjct: 364 YESAIFGTFLPFFIASSSTEIDISALFGWEGLNGIILIMVTSFVVKFIFTTVPALFYGMP 423

Query: 417 VRHGVSLGLVLNSQGALELGIFKLMRKNKVINDEAFVVMCICVMVLVAVITPIIGHLFDP 476
           +    +L L+++ +G  ELG + L  +   +  E F V C+ + +  A+I PI+ +L+DP
Sbjct: 424 MEDCFALSLIMSFKGIFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDP 483

Query: 477 SRRYVVHKKRTVMHSRPEIDLCVLVCIHDQEDVPSAINLLEALNPTRRSHLIVYMLHLVE 536
           SR Y  ++KR + H +P  +L +L CI+  +D+   INLLEA+ P+R S +  Y+LHL+E
Sbjct: 484 SRMYAGYEKRNMQHLKPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLME 543

Query: 537 LLGRANPQLIHHKLTKIRTSRSCPSEHIVNAFKHFGQSNREIVAIHPFTAISPSATMYDD 596
           L+G+ANP  I HKL   RT  +  S +++ +F+ F +     V +  +TA+S   TM+ D
Sbjct: 544 LVGQANPIFISHKLQTRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGD 603

Query: 597 VCSLALDKRSSLILVPFHKRFHSNGVMSLSKSKM-KMVNHHIFDKAPCSVAVVVER---G 656
           +C LAL+  +SLIL+PFH+ + ++G   +S + M + +N  + D APCSV V V R   G
Sbjct: 604 ICMLALNNTTSLILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSG 663

Query: 657 IFNVTRSIAT------NLYSFQVATVFLGGPDDREAIFIGARMAGHSNINLTVIRLL-ED 716
             N++    T      NL S+ +  +FLGG DDREA+ +  RMA    IN+T++RL+  D
Sbjct: 664 RKNISSGRKTINGTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTD 723

Query: 717 GNVSSDDVKERRLDNEAVVEFRQMMANN-YKVRYIEEVVKDGTGVVSVLRTMGNNFDLVM 776
                + V ++ LD+E +   R + +N    + Y E+ ++D     S+LR+M ++FD+ +
Sbjct: 724 EKARENTVWDKMLDDELL---RDVKSNTLVDIFYSEKAIEDAAETSSLLRSMVSDFDMFI 783

Query: 777 VGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMILVVQQHTKVV 780
           VGR +       +GL  W+E  ELG IG++L S DF     +LV+QQ   ++
Sbjct: 784 VGRGNGRTSVFTEGLEEWSEFKELGIIGDLLTSQDFNCQASVLVIQQQQLMI 822

BLAST of Tan0022359 vs. NCBI nr
Match: XP_022983542.1 (cation/H(+) antiporter 15-like [Cucurbita maxima])

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 584/806 (72.46%), Postives = 678/806 (84.12%), Query Frame = 0

Query: 1   MGSIVMEPGDIAIF----------KNFTSICTYADRIRCTGVFTGANPLEFSVPLLLLQL 60
           MGSI+MEP D+A +          KN T ICT+ADRI  TGVF+G NPLEFSVPLL LQL
Sbjct: 1   MGSIIMEPDDVAAYISGCDSHNTLKNITRICTFADRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GVSAGTIVLFAWLLKPLGQPLIVSQILVGLVLGSSGLSHLENFRETIFPLRGFICLDAVS 120
           G+SAGTI+LF+ LLKPLGQPLIVSQIL G+VLGSSGL HL  FRE IFPLRGF+ LD +S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGQIFYFFLIGVQTDISIMKNIDRRAFGIGFCSVILPMILITIYPIILVNIVDFKFVKS 180
           ALG +F+FFLIGVQTDIS +K ID++AF IG CSVIL MIL TIY I LVNIVD + VK 
Sbjct: 121 ALGHVFFFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LFVLGKIETFINFPMVASLLSELHLINSEFGRIALSSSMVSNFLSIFFLLIGAILFPQSV 240
           LF +G  E+FINFPMVASL+SELHLINSEFGR ALS++M SNF SI   L+GA+L PQ  
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLTPQDE 240

Query: 241 TGSKILSTTYASAVVVVVIFFAIRPIVLWMVKKNPIGQQLKEGFVITLLLGVLVVAFCSQ 300
              + +ST YAS ++V VIFFAIRP ++WM+KKNPIGQ LKE FV+TLLL VLVVAFC Q
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 ASGLHIYFGPLLLGITIPPGPPIGSALVERLDLITSWVFMPVFFVKIGLVVNIYTIKLKN 360
           A GLHIY GPL LG+TIP GPPIGSALV+RLD ITSWVFMP+FFVKIGL VNIY IKL N
Sbjct: 301 ALGLHIYTGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLAVNIYAIKLIN 360

Query: 361 FLCISFIILVGALGKFLGALVISLYYKLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVI 420
           FLC+SFI+ V ALGKFLGAL+IS Y+KL +R  VSLGL+LN QGALELG+FK+MRK K+I
Sbjct: 361 FLCMSFIVFVNALGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDEAFVVMCICVMVLVAVITPIIGHLFDPSRRYVVHKKRTVMHSRPEIDLCVLVCIHDQE 480
           NDEA VVMC+ VMV+VA+I+PII HL DPSRR++VHK+RTVMHSRPE DLCVLVCIHDQE
Sbjct: 421 NDEALVVMCVWVMVVVAIISPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCVLVCIHDQE 480

Query: 481 DVPSAINLLEALNPTRRSHLIVYMLHLVELLGRANPQLIHHKLTKIRTSRSCPSEHIVNA 540
           DVPSAINLL+ALNPTRRSHL+VY+LHLVELLGRA+ +LI+HK  ++RTSRSC S  IVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKHFGQSNREIVAIHPFTAISPSATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSK 600
           FK+FG+S REI+AI+PFTAISPS TM+D VCSLALDK++SLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKREILAIYPFTAISPSTTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 SKMKMVNHHIFDKAPCSVAVVVERGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGA 660
            K KMVNHHI + APCSVA+VVERGI    +SIATN + FQ+  VF+GGPDDREA+FIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIVMVFIGGPDDREAMFIGA 660

Query: 661 RMAGHSNINLTVIRLLEDGNVSSDDVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDGT 720
           RM GH NINLT IRL E GNV S DVKERRLDNE+V EFRQ +A+N +V+YIEEVVKDG 
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEVVKDGI 720

Query: 721 GVVSVLRTMGNNFDLVMVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMIL 780
           G +S+LR++G +FDLV+VGR+H+ CLALVQGLV WNEQTELGAIGEVLASSDF+GN  IL
Sbjct: 721 GTISILRSLGGDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTKVVNEEEENHFGTNILMDNT 797
           VVQQH +V+NE+EE +    I MDNT
Sbjct: 781 VVQQHRRVINEDEELNQEDIIPMDNT 806

BLAST of Tan0022359 vs. NCBI nr
Match: XP_022934362.1 (cation/H(+) antiporter 15-like [Cucurbita moschata])

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 584/806 (72.46%), Postives = 680/806 (84.37%), Query Frame = 0

Query: 1   MGSIVMEPGDIAIF----------KNFTSICTYADRIRCTGVFTGANPLEFSVPLLLLQL 60
           MGSI+MEP D+A +          KN T ICT+A+RI  TGVF+G NPLEFSVPLL LQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGQNTLKNLTRICTFANRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GVSAGTIVLFAWLLKPLGQPLIVSQILVGLVLGSSGLSHLENFRETIFPLRGFICLDAVS 120
           G+SAGTI+LF+ LLKPLGQPLIVSQIL G+VLGSSGL HL  FRE IFPLRGF+ LD +S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGQIFYFFLIGVQTDISIMKNIDRRAFGIGFCSVILPMILITIYPIILVNIVDFKFVKS 180
           ALG +FYFFLIGVQTDIS +K ID++AF IG CSVIL MIL TIY I LVNIVD + VK 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LFVLGKIETFINFPMVASLLSELHLINSEFGRIALSSSMVSNFLSIFFLLIGAILFPQSV 240
           LF +G  E+FINFPMVASL+SELHLINSEFGR ALS++M SNF SI   L+GA+L PQS 
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLAPQSE 240

Query: 241 TGSKILSTTYASAVVVVVIFFAIRPIVLWMVKKNPIGQQLKEGFVITLLLGVLVVAFCSQ 300
              + +ST YAS ++V VIFFAIRP ++WM+KKNPIGQ LKE FV+TLLL VLVVAFC Q
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 ASGLHIYFGPLLLGITIPPGPPIGSALVERLDLITSWVFMPVFFVKIGLVVNIYTIKLKN 360
           ASGLHIY GPL LG+TIP GPPIGSALV+RLD ITSWVFMP+FFVKIGLVVNIY  KL N
Sbjct: 301 ASGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCISFIILVGALGKFLGALVISLYYKLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVI 420
           FLC+SFI+ V A+GKFLGAL+IS Y+KL +R  VSLGL+LN QGALELG+FK+MRK K+I
Sbjct: 361 FLCMSFIVFVSAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDEAFVVMCICVMVLVAVITPIIGHLFDPSRRYVVHKKRTVMHSRPEIDLCVLVCIHDQE 480
           NDEA VV C+ VMV+VA+ITPII HL DPSRR++VHK+RTVMHSRPE DLC LVCIHDQE
Sbjct: 421 NDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCALVCIHDQE 480

Query: 481 DVPSAINLLEALNPTRRSHLIVYMLHLVELLGRANPQLIHHKLTKIRTSRSCPSEHIVNA 540
           DVPSAINLL+ALNPTRRSHL+VY+LHLVELLGRA+ +LI+HK  ++RTSRSC S  IVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKHFGQSNREIVAIHPFTAISPSATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSK 600
           FK+FG+S  EI+AI+PFTAISPS+TM+D VCSLALDK++SLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKSEILAIYPFTAISPSSTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 SKMKMVNHHIFDKAPCSVAVVVERGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGA 660
            K KMVNHHI + APCSVA+VVERGI    +SIATN + FQ+A VF+GGPDDREA+FIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIAMVFIGGPDDREAMFIGA 660

Query: 661 RMAGHSNINLTVIRLLEDGNVSSDDVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDGT 720
           RM GH NINLT IRL E GNV S DVKERRLDNE+V EFRQ +A+N +V+YIEE+VKDG 
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEMVKDGI 720

Query: 721 GVVSVLRTMGNNFDLVMVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMIL 780
           G +S+LR++G++FDLV+VGR+H+ CLALVQGLV WNEQTELGAIGEVLASSDF+GN  IL
Sbjct: 721 GTISILRSLGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTKVVNEEEENHFGTNILMDNT 797
           VVQQH +VVNE+EE +    I MDNT
Sbjct: 781 VVQQHRRVVNEDEELNQEDIIPMDNT 806

BLAST of Tan0022359 vs. NCBI nr
Match: XP_023528659.1 (cation/H(+) antiporter 15-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1129.8 bits (2921), Expect = 0.0e+00
Identity = 582/806 (72.21%), Postives = 679/806 (84.24%), Query Frame = 0

Query: 1   MGSIVMEPGDIAIF----------KNFTSICTYADRIRCTGVFTGANPLEFSVPLLLLQL 60
           MGSI+MEP D+A +          KN T ICT+A+RI   GVF+G NPLEFSVPLL LQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGHNTLKNLTKICTFANRIHSPGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GVSAGTIVLFAWLLKPLGQPLIVSQILVGLVLGSSGLSHLENFRETIFPLRGFICLDAVS 120
           G+SAGTI+ F+ LLKPLGQPLIVSQIL G+VLGSSGL HL  FRE IFPLRGF+ LD +S
Sbjct: 61  GLSAGTIIFFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGQIFYFFLIGVQTDISIMKNIDRRAFGIGFCSVILPMILITIYPIILVNIVDFKFVKS 180
           ALG +FYFFLIGVQTDIS +K ID++AF IG CSVIL MIL TIY I LVNIVD + +K 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTIKY 180

Query: 181 LFVLGKIETFINFPMVASLLSELHLINSEFGRIALSSSMVSNFLSIFFLLIGAILFPQSV 240
           LF +G  E+FINFPMVASL+SELHLINSEFGR ALS++M SNF SI   L+GA+L PQ+ 
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLAPQNE 240

Query: 241 TGSKILSTTYASAVVVVVIFFAIRPIVLWMVKKNPIGQQLKEGFVITLLLGVLVVAFCSQ 300
              + +ST YAS ++V VIFFAIRPI++WM+KKNPIG+ LKE FV+TLLL VLVVAFC Q
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPIIVWMIKKNPIGKPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 ASGLHIYFGPLLLGITIPPGPPIGSALVERLDLITSWVFMPVFFVKIGLVVNIYTIKLKN 360
           A GLHIY GPL LG+TIP GPPIGSALV+RLD ITSWVFMP+FFVKIGLVVNIY  KL N
Sbjct: 301 ALGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCISFIILVGALGKFLGALVISLYYKLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVI 420
           FLC+SFI+ VGA+GKFLGAL+IS Y+KL +R  VSLGL+LN QGALELG+FK+MRK K+I
Sbjct: 361 FLCMSFIVFVGAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDEAFVVMCICVMVLVAVITPIIGHLFDPSRRYVVHKKRTVMHSRPEIDLCVLVCIHDQE 480
           NDEA VV C+ VMV+VA+ITPII HL DPSRR++VHK+RTVMHSRPE DLCVLVCIHDQE
Sbjct: 421 NDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCVLVCIHDQE 480

Query: 481 DVPSAINLLEALNPTRRSHLIVYMLHLVELLGRANPQLIHHKLTKIRTSRSCPSEHIVNA 540
           DVPSAINLL+ALNPTRRSHL VY+LHLVELLGRA+ +LIHHK  ++RTSRSC S  IVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLAVYILHLVELLGRAHLELIHHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKHFGQSNREIVAIHPFTAISPSATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSK 600
           FK+FG+S  EI+AI+PFTAISPSATM+D VCSLALDK++SLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKSEILAIYPFTAISPSATMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 SKMKMVNHHIFDKAPCSVAVVVERGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGA 660
            K KMVNHHI + APCS+A+VVERGI    +SIATN + FQ+A VF+GGPDDREA+FIGA
Sbjct: 601 YKTKMVNHHILENAPCSIALVVERGILKTPKSIATNFHPFQIAMVFIGGPDDREAMFIGA 660

Query: 661 RMAGHSNINLTVIRLLEDGNVSSDDVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDGT 720
           RM GH NINLT IRL E GNV S DVKERRLDN++V EFRQ +A+N +V+YIEE+VKDG 
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNKSVDEFRQSIADNNRVKYIEEMVKDGI 720

Query: 721 GVVSVLRTMGNNFDLVMVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMIL 780
           G +S+LR+MG++FDLV+VGR+H+ CLALVQGLV WNEQTELGAIGEVLASSDF+GN  IL
Sbjct: 721 GTISILRSMGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTKVVNEEEENHFGTNILMDNT 797
           VVQQH +VVNE+EE +    I MDNT
Sbjct: 781 VVQQHRRVVNEDEELNQEDIIPMDNT 806

BLAST of Tan0022359 vs. NCBI nr
Match: KAG6581108.1 (Cation/H(+) antiporter 15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1124.4 bits (2907), Expect = 0.0e+00
Identity = 580/806 (71.96%), Postives = 679/806 (84.24%), Query Frame = 0

Query: 1   MGSIVMEPGDIAIF----------KNFTSICTYADRIRCTGVFTGANPLEFSVPLLLLQL 60
           MGSI+MEP D+A +          KN T ICT+A+RI  TGVF+G NPLEFSVPLL LQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGQNTLKNLTRICTFANRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GVSAGTIVLFAWLLKPLGQPLIVSQILVGLVLGSSGLSHLENFRETIFPLRGFICLDAVS 120
           G+SAGTI+LF+ LLKPLGQPLIVSQIL G+VLGSSGL HL  FRE IFPLRGF+ LD +S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGQIFYFFLIGVQTDISIMKNIDRRAFGIGFCSVILPMILITIYPIILVNIVDFKFVKS 180
           ALG +FYFFLIGVQTDIS +K ID++AF IG CSVIL MIL TIY I LVNIVD + VK 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LFVLGKIETFINFPMVASLLSELHLINSEFGRIALSSSMVSNFLSIFFLLIGAILFPQSV 240
           LF +G  E+ INFPMVASL+SELHLINSEFGR++LS++M SNF SI   L+GA+L PQS 
Sbjct: 181 LFAIGGTESLINFPMVASLVSELHLINSEFGRLSLSTAMASNFFSICLALLGALLAPQSE 240

Query: 241 TGSKILSTTYASAVVVVVIFFAIRPIVLWMVKKNPIGQQLKEGFVITLLLGVLVVAFCSQ 300
              + +ST YAS ++V VIFFAIRP ++WM+KKNPIGQ LKE FV+TLLL VLVVAFC Q
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 ASGLHIYFGPLLLGITIPPGPPIGSALVERLDLITSWVFMPVFFVKIGLVVNIYTIKLKN 360
           A GLHIY GPL LG+TIP GPPIGSALV+RLD ITSWVFMP+FFVKIGLVVNIY  KL N
Sbjct: 301 ALGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCISFIILVGALGKFLGALVISLYYKLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVI 420
           FLC+SFI+ V A+GKFLGAL+IS Y+KL +R  VSLGL+LN QGALELG+FK+MRK K+I
Sbjct: 361 FLCMSFIVFVSAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDEAFVVMCICVMVLVAVITPIIGHLFDPSRRYVVHKKRTVMHSRPEIDLCVLVCIHDQE 480
           NDEA VV C+ VMV+VA+ITPII HL DPSRR++VHK+RTVMHSRPE DLC LVCIHDQE
Sbjct: 421 NDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFLVHKRRTVMHSRPEFDLCALVCIHDQE 480

Query: 481 DVPSAINLLEALNPTRRSHLIVYMLHLVELLGRANPQLIHHKLTKIRTSRSCPSEHIVNA 540
           DVPSAINLL+ALNPTRRSHL+V++LHLVELLGRA+ +LI+HK  ++RTSRSC S  IVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVHILHLVELLGRAHVELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKHFGQSNREIVAIHPFTAISPSATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSK 600
           FK+FG+S  EI+AI+PFTAISPS+TM+D VCSLALDK++SLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKSEILAIYPFTAISPSSTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 SKMKMVNHHIFDKAPCSVAVVVERGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGA 660
            K KMVNHHI + APCSVA+VVERGI    +SIATN + FQ+A VF+GGPDDREA+FIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIAMVFIGGPDDREAMFIGA 660

Query: 661 RMAGHSNINLTVIRLLEDGNVSSDDVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDGT 720
           RM GH NINLT IRL E GNV S DVKERRLDNE+V EFRQ +A+N +V+YIEE+VKDG 
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEMVKDGI 720

Query: 721 GVVSVLRTMGNNFDLVMVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMIL 780
           G +S+LR++G++FDLV+VGR+H+ CLALVQGLV WNEQTELGAIGEVLASSDF+GN  IL
Sbjct: 721 GTISILRSLGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTKVVNEEEENHFGTNILMDNT 797
           VVQQH +VVNE+EE +    I MDNT
Sbjct: 781 VVQQHRRVVNEDEELNQEDIIPMDNT 806

BLAST of Tan0022359 vs. NCBI nr
Match: KAG7017840.1 (Cation/H(+) antiporter 15 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1115.5 bits (2884), Expect = 0.0e+00
Identity = 580/818 (70.90%), Postives = 679/818 (83.01%), Query Frame = 0

Query: 1   MGSIVMEPGDIAIF----------KNFTSICTYADRIRCTGVFTGANPLEFSVPLLLLQL 60
           MGSI+MEP D+A +          KN T ICT+A+RI  TGVF+G NPLEFSVPLL LQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGQNTLKNLTRICTFANRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GVSAGTIVLFAWLLKPLGQPLIVSQILVGLVLGSSGLSHLENFRETIFPLRGFICLDAVS 120
           G+SAGTI+LF+ LLKPLGQPLIVSQIL G+VLGSSGL HL  FRE IFPLRGF+ LD +S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGQIFYFFLIGVQTDISIMKNIDRRAFGIGFCSVILPMILITIYPIILVNIVDFKFVKS 180
           ALG +FYFFLIGVQTDIS +K ID++AF IG CSVIL MIL TIY I LVNIVD + VK 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LFVLGKIETFINFPMVASLLSELHLINSEFGRIALSSSMVSNFLSIFFLLIGAILFPQSV 240
           LF +G  E+ INFPMVASL+SELHLINSEFGR++LS++M SNF SI   L+GA+L PQS 
Sbjct: 181 LFAIGGTESLINFPMVASLVSELHLINSEFGRLSLSTAMASNFFSICLALLGALLAPQSE 240

Query: 241 TGSKILSTTYASAVVVVVIFFAIRPIVLWMVKKNPIGQQLKEGFVITLLLGVLVVAFCSQ 300
              + +ST YAS ++V VIFFAIRP ++WM+KKNPIGQ LKE FV+TLLL VLVVAFC Q
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 ASGLHIYFGPLLLGITIPPGPPIGSALVERLDLITSWVFMPVFFVKIGLVVNIYTIKLKN 360
           A GLHIY GPL LG+TIP GPPIGSALV+RLD ITSWVFMP+FFVKIGLVVNIY  KL N
Sbjct: 301 ALGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCISFIILVGALGKFLGALVISLYYKLPVRHGVSLGLVLNSQGALELGIFKLMRKNK-- 420
           FLC+SFI+ V A+GKFLGAL+IS Y+KL +R  VSLGL+LN QGALELG+FK+MRK K  
Sbjct: 361 FLCMSFIVFVSAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKEL 420

Query: 421 ----------VINDEAFVVMCICVMVLVAVITPIIGHLFDPSRRYVVHKKRTVMHSRPEI 480
                     +INDEA VV C+ VMV+VA+ITPII HL DPSRR++VHK+RTVMHSRPE 
Sbjct: 421 IASLFLESTQLINDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFLVHKRRTVMHSRPEF 480

Query: 481 DLCVLVCIHDQEDVPSAINLLEALNPTRRSHLIVYMLHLVELLGRANPQLIHHKLTKIRT 540
           DLC LVCIHDQEDVPSAINLL+ALNPTRRSHL+VY+LHLVELLGRA+ +LI+HK  ++RT
Sbjct: 481 DLCALVCIHDQEDVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRT 540

Query: 541 SRSCPSEHIVNAFKHFGQSNREIVAIHPFTAISPSATMYDDVCSLALDKRSSLILVPFHK 600
           SRSC S  IVNAFK+FG+S  EI+AI+PFTAISPS+TM+D VCSLALDK++SLILVPFH+
Sbjct: 541 SRSCSSIPIVNAFKYFGESKSEILAIYPFTAISPSSTMHDVVCSLALDKKTSLILVPFHQ 600

Query: 601 RFHSNGVMSLSKSKMKMVNHHIFDKAPCSVAVVVERGIFNVTRSIATNLYSFQVATVFLG 660
           RFHSNGV+SLS+ K KMVNHHI + APCSVA+VVERGI    +SIA+N + FQ+A VF+G
Sbjct: 601 RFHSNGVLSLSQYKTKMVNHHILENAPCSVALVVERGILKTPKSIASNFHPFQIAMVFIG 660

Query: 661 GPDDREAIFIGARMAGHSNINLTVIRLLEDGNVSSDDVKERRLDNEAVVEFRQMMANNYK 720
           GPDDREA+FIGARM GH NINLT IRL E GNV S DVKERRLDNE+V EFRQ +A+N +
Sbjct: 661 GPDDREAMFIGARMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNR 720

Query: 721 VRYIEEVVKDGTGVVSVLRTMGNNFDLVMVGRRHSSCLALVQGLVFWNEQTELGAIGEVL 780
           V+YIEE+VKDG G +S+LR++G++FDLV+VGR+H+ CLALVQGLV WNEQTELGAIGEVL
Sbjct: 721 VKYIEEMVKDGIGTISILRSLGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVL 780

Query: 781 ASSDFMGNTMILVVQQHTKVVNEEEENHFGTNILMDNT 797
           ASSDF+GN  ILVVQQH +VVNE+EE +    I MDNT
Sbjct: 781 ASSDFIGNATILVVQQHRRVVNEDEELNQEDIIPMDNT 818

BLAST of Tan0022359 vs. ExPASy TrEMBL
Match: A0A6J1IZN7 (cation/H(+) antiporter 15-like OS=Cucurbita maxima OX=3661 GN=LOC111482115 PE=4 SV=1)

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 584/806 (72.46%), Postives = 678/806 (84.12%), Query Frame = 0

Query: 1   MGSIVMEPGDIAIF----------KNFTSICTYADRIRCTGVFTGANPLEFSVPLLLLQL 60
           MGSI+MEP D+A +          KN T ICT+ADRI  TGVF+G NPLEFSVPLL LQL
Sbjct: 1   MGSIIMEPDDVAAYISGCDSHNTLKNITRICTFADRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GVSAGTIVLFAWLLKPLGQPLIVSQILVGLVLGSSGLSHLENFRETIFPLRGFICLDAVS 120
           G+SAGTI+LF+ LLKPLGQPLIVSQIL G+VLGSSGL HL  FRE IFPLRGF+ LD +S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGQIFYFFLIGVQTDISIMKNIDRRAFGIGFCSVILPMILITIYPIILVNIVDFKFVKS 180
           ALG +F+FFLIGVQTDIS +K ID++AF IG CSVIL MIL TIY I LVNIVD + VK 
Sbjct: 121 ALGHVFFFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LFVLGKIETFINFPMVASLLSELHLINSEFGRIALSSSMVSNFLSIFFLLIGAILFPQSV 240
           LF +G  E+FINFPMVASL+SELHLINSEFGR ALS++M SNF SI   L+GA+L PQ  
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLTPQDE 240

Query: 241 TGSKILSTTYASAVVVVVIFFAIRPIVLWMVKKNPIGQQLKEGFVITLLLGVLVVAFCSQ 300
              + +ST YAS ++V VIFFAIRP ++WM+KKNPIGQ LKE FV+TLLL VLVVAFC Q
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 ASGLHIYFGPLLLGITIPPGPPIGSALVERLDLITSWVFMPVFFVKIGLVVNIYTIKLKN 360
           A GLHIY GPL LG+TIP GPPIGSALV+RLD ITSWVFMP+FFVKIGL VNIY IKL N
Sbjct: 301 ALGLHIYTGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLAVNIYAIKLIN 360

Query: 361 FLCISFIILVGALGKFLGALVISLYYKLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVI 420
           FLC+SFI+ V ALGKFLGAL+IS Y+KL +R  VSLGL+LN QGALELG+FK+MRK K+I
Sbjct: 361 FLCMSFIVFVNALGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDEAFVVMCICVMVLVAVITPIIGHLFDPSRRYVVHKKRTVMHSRPEIDLCVLVCIHDQE 480
           NDEA VVMC+ VMV+VA+I+PII HL DPSRR++VHK+RTVMHSRPE DLCVLVCIHDQE
Sbjct: 421 NDEALVVMCVWVMVVVAIISPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCVLVCIHDQE 480

Query: 481 DVPSAINLLEALNPTRRSHLIVYMLHLVELLGRANPQLIHHKLTKIRTSRSCPSEHIVNA 540
           DVPSAINLL+ALNPTRRSHL+VY+LHLVELLGRA+ +LI+HK  ++RTSRSC S  IVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKHFGQSNREIVAIHPFTAISPSATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSK 600
           FK+FG+S REI+AI+PFTAISPS TM+D VCSLALDK++SLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKREILAIYPFTAISPSTTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 SKMKMVNHHIFDKAPCSVAVVVERGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGA 660
            K KMVNHHI + APCSVA+VVERGI    +SIATN + FQ+  VF+GGPDDREA+FIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIVMVFIGGPDDREAMFIGA 660

Query: 661 RMAGHSNINLTVIRLLEDGNVSSDDVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDGT 720
           RM GH NINLT IRL E GNV S DVKERRLDNE+V EFRQ +A+N +V+YIEEVVKDG 
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEVVKDGI 720

Query: 721 GVVSVLRTMGNNFDLVMVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMIL 780
           G +S+LR++G +FDLV+VGR+H+ CLALVQGLV WNEQTELGAIGEVLASSDF+GN  IL
Sbjct: 721 GTISILRSLGGDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTKVVNEEEENHFGTNILMDNT 797
           VVQQH +V+NE+EE +    I MDNT
Sbjct: 781 VVQQHRRVINEDEELNQEDIIPMDNT 806

BLAST of Tan0022359 vs. ExPASy TrEMBL
Match: A0A6J1F2I8 (cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111441553 PE=4 SV=1)

HSP 1 Score: 1131.7 bits (2926), Expect = 0.0e+00
Identity = 584/806 (72.46%), Postives = 680/806 (84.37%), Query Frame = 0

Query: 1   MGSIVMEPGDIAIF----------KNFTSICTYADRIRCTGVFTGANPLEFSVPLLLLQL 60
           MGSI+MEP D+A +          KN T ICT+A+RI  TGVF+G NPLEFSVPLL LQL
Sbjct: 1   MGSIIMEPDDVAAYISGGHGQNTLKNLTRICTFANRIHSTGVFSGVNPLEFSVPLLFLQL 60

Query: 61  GVSAGTIVLFAWLLKPLGQPLIVSQILVGLVLGSSGLSHLENFRETIFPLRGFICLDAVS 120
           G+SAGTI+LF+ LLKPLGQPLIVSQIL G+VLGSSGL HL  FRE IFPLRGF+ LD +S
Sbjct: 61  GLSAGTIILFSMLLKPLGQPLIVSQILGGVVLGSSGLGHLGRFREVIFPLRGFVVLDVIS 120

Query: 121 ALGQIFYFFLIGVQTDISIMKNIDRRAFGIGFCSVILPMILITIYPIILVNIVDFKFVKS 180
           ALG +FYFFLIGVQTDIS +K ID++AF IG CSVIL MIL TIY I LVNIVD + VK 
Sbjct: 121 ALGHVFYFFLIGVQTDISFVKKIDKKAFAIGSCSVILSMILSTIYSITLVNIVDIQTVKY 180

Query: 181 LFVLGKIETFINFPMVASLLSELHLINSEFGRIALSSSMVSNFLSIFFLLIGAILFPQSV 240
           LF +G  E+FINFPMVASL+SELHLINSEFGR ALS++M SNF SI   L+GA+L PQS 
Sbjct: 181 LFAIGGTESFINFPMVASLVSELHLINSEFGRFALSTAMASNFFSICLALLGALLAPQSE 240

Query: 241 TGSKILSTTYASAVVVVVIFFAIRPIVLWMVKKNPIGQQLKEGFVITLLLGVLVVAFCSQ 300
              + +ST YAS ++V VIFFAIRP ++WM+KKNPIGQ LKE FV+TLLL VLVVAFC Q
Sbjct: 241 GKYQTISTLYASLMLVAVIFFAIRPTIVWMIKKNPIGQPLKECFVVTLLLLVLVVAFCCQ 300

Query: 301 ASGLHIYFGPLLLGITIPPGPPIGSALVERLDLITSWVFMPVFFVKIGLVVNIYTIKLKN 360
           ASGLHIY GPL LG+TIP GPPIGSALV+RLD ITSWVFMP+FFVKIGLVVNIY  KL N
Sbjct: 301 ASGLHIYIGPLFLGVTIPSGPPIGSALVDRLDFITSWVFMPIFFVKIGLVVNIYATKLIN 360

Query: 361 FLCISFIILVGALGKFLGALVISLYYKLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVI 420
           FLC+SFI+ V A+GKFLGAL+IS Y+KL +R  VSLGL+LN QGALELG+FK+MRK K+I
Sbjct: 361 FLCMSFIVFVSAMGKFLGALLISTYFKLSMRDAVSLGLILNCQGALELGVFKIMRKEKLI 420

Query: 421 NDEAFVVMCICVMVLVAVITPIIGHLFDPSRRYVVHKKRTVMHSRPEIDLCVLVCIHDQE 480
           NDEA VV C+ VMV+VA+ITPII HL DPSRR++VHK+RTVMHSRPE DLC LVCIHDQE
Sbjct: 421 NDEALVVTCVWVMVVVAIITPIIRHLLDPSRRFIVHKRRTVMHSRPEFDLCALVCIHDQE 480

Query: 481 DVPSAINLLEALNPTRRSHLIVYMLHLVELLGRANPQLIHHKLTKIRTSRSCPSEHIVNA 540
           DVPSAINLL+ALNPTRRSHL+VY+LHLVELLGRA+ +LI+HK  ++RTSRSC S  IVNA
Sbjct: 481 DVPSAINLLDALNPTRRSHLVVYILHLVELLGRAHLELIYHKQMEVRTSRSCSSIPIVNA 540

Query: 541 FKHFGQSNREIVAIHPFTAISPSATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSK 600
           FK+FG+S  EI+AI+PFTAISPS+TM+D VCSLALDK++SLILVPFH+RFHSNGV+SLS+
Sbjct: 541 FKYFGESKSEILAIYPFTAISPSSTMHDVVCSLALDKKTSLILVPFHQRFHSNGVLSLSQ 600

Query: 601 SKMKMVNHHIFDKAPCSVAVVVERGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGA 660
            K KMVNHHI + APCSVA+VVERGI    +SIATN + FQ+A VF+GGPDDREA+FIGA
Sbjct: 601 YKTKMVNHHILENAPCSVALVVERGILKTPKSIATNFHPFQIAMVFIGGPDDREAMFIGA 660

Query: 661 RMAGHSNINLTVIRLLEDGNVSSDDVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDGT 720
           RM GH NINLT IRL E GNV S DVKERRLDNE+V EFRQ +A+N +V+YIEE+VKDG 
Sbjct: 661 RMVGHPNINLTFIRLQESGNVPSSDVKERRLDNESVNEFRQSIADNNRVKYIEEMVKDGI 720

Query: 721 GVVSVLRTMGNNFDLVMVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMIL 780
           G +S+LR++G++FDLV+VGR+H+ CLALVQGLV WNEQTELGAIGEVLASSDF+GN  IL
Sbjct: 721 GTISILRSLGSDFDLVIVGRQHNPCLALVQGLVLWNEQTELGAIGEVLASSDFIGNATIL 780

Query: 781 VVQQHTKVVNEEEENHFGTNILMDNT 797
           VVQQH +VVNE+EE +    I MDNT
Sbjct: 781 VVQQHRRVVNEDEELNQEDIIPMDNT 806

BLAST of Tan0022359 vs. ExPASy TrEMBL
Match: A0A6J1EN94 (cation/H(+) antiporter 15-like OS=Cucurbita moschata OX=3662 GN=LOC111435106 PE=4 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 555/785 (70.70%), Postives = 655/785 (83.44%), Query Frame = 0

Query: 1   MGSIVMEPGDIAIFKNFTSICTYADRIRCTGVFTGANPLEFSVPLLLLQLGVSAGTIVLF 60
           MGSIVMEPGD +IF NF+SIC + DRI    VF G NPLEFSVPLLLLQLG+ +GTI+  
Sbjct: 1   MGSIVMEPGDNSIFNNFSSICVHVDRIHSNSVFIGENPLEFSVPLLLLQLGICSGTIIFL 60

Query: 61  AWLLKPLGQPLIVSQILVGLVLGSSGLSHLENFRETIFPLRGFICLDAVSALGQIFYFFL 120
             LLK L QPLIVSQIL GLVLG  G   LE FRETIFP+RG I LDA+SALGQ+FYFFL
Sbjct: 61  FQLLKRLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISALGQVFYFFL 120

Query: 121 IGVQTDISIMKNIDRRAFGIGFCSVILPMILITIYPIILVNIVDFKFVKSLFVLGKIETF 180
           IGVQTD+SI+KNID+RAFGIG CSVILP IL  +Y + LVN VD   +K+LFV+G  E F
Sbjct: 121 IGVQTDMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFVVGNAECF 180

Query: 181 INFPMVASLLSELHLINSEFGRIALSSSMVSNFLSIFFLLIGAILFPQSVTGSKILSTTY 240
           I+F MVASLL+ELHLINS+FG+IAL+SSM SN   +FF  IG +L  +       LST Y
Sbjct: 181 IHFAMVASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY 240

Query: 241 ASAVVVVVIFFAIRPIVLWMVKKNPIGQQLKEGFVITLLLGVLVVAFCSQASGLHIYFGP 300
              +V+ VI  + +P++LWM K +P+GQ LKE FVITL LGVLVVAFCSQASG+HIYFGP
Sbjct: 241 -GFIVLAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGP 300

Query: 301 LLLGITIPPGPPIGSALVERLDLITSWVFMPVFFVKIGLVVNIYTIKLKNFLCISFIILV 360
           +LLGITIPPGPPIGSA+VE+LD+I SWV MP+FF KIGL+ +I+TIKL NFLCISFII+V
Sbjct: 301 ILLGITIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLIFSIHTIKLLNFLCISFIIIV 360

Query: 361 GALGKFLGALVISLYYKLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVINDEAFVVMCI 420
            A GKFLGAL++S+YYKLP R GV+LGL+LNSQGALE+GIFKLM+K+KV+++E FVVMCI
Sbjct: 361 AAFGKFLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIFKLMKKDKVLDEETFVVMCI 420

Query: 421 CVMVLVAVITPIIGHLFDPSRRYVVHKKRTVMHSRPEIDLCVLVCIHDQEDVPSAINLLE 480
           CVM++ AVITPI+ +LFDPSRR+V  KKRTVMH RPE DL VLVCIHDQ+DVPSAIN+LE
Sbjct: 421 CVMLVAAVITPILRYLFDPSRRFVTEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILE 480

Query: 481 ALNPTRRSHLIVYMLHLVELLGRANPQLIHHKLTKIRT-SRSCPSEHIVNAFKHFGQSNR 540
           AL PT RSHLIVYMLHLV LLGRANPQLIHH++TK+RT SRS  SE IVNAF +FGQSN 
Sbjct: 481 ALKPTHRSHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSYVSESIVNAFNYFGQSNS 540

Query: 541 EIVAIHPFTAISPSATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSKSKMKMVNHH 600
           ++V IHPFTA+SPS TMY+DVCSLALDKR+SLIL+PFHKRFHSNG+MSLSK KMKM+N H
Sbjct: 541 DVVTIHPFTAVSPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMLNQH 600

Query: 601 IFDKAPCSVAVVVERGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGARMAGHSNIN 660
           I DKAPCSVA++VERGI  ++RSI+TNLYSFQ+A VF+GGPDDREAIFIG RM GH  IN
Sbjct: 601 ILDKAPCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYIN 660

Query: 661 LTVIRLLEDGNVSSDDVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDGTGVVSVLRTM 720
           +T+IRLLED N  SD  KE +L+NEAV ++ ++M NN++VRY+EEV KD TG V++LRT+
Sbjct: 661 VTLIRLLEDTNSPSDGGKEMKLNNEAVSKYHRLMENNHRVRYLEEVAKDCTGTVAILRTL 720

Query: 721 GNNFDLVMVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMILVVQQHTKVV 780
            NNFDL++VGRRHS    LVQGL+ WNEQTELG+IGEVLASSDF+GN  I VVQQH KVV
Sbjct: 721 DNNFDLILVGRRHSPRSPLVQGLLLWNEQTELGSIGEVLASSDFIGNATIFVVQQHRKVV 780

Query: 781 NEEEE 785
           NE+ E
Sbjct: 781 NEDPE 784

BLAST of Tan0022359 vs. ExPASy TrEMBL
Match: A0A6J1JQQ2 (cation/H(+) antiporter 15-like OS=Cucurbita maxima OX=3661 GN=LOC111486629 PE=4 SV=1)

HSP 1 Score: 1076.2 bits (2782), Expect = 0.0e+00
Identity = 550/780 (70.51%), Postives = 652/780 (83.59%), Query Frame = 0

Query: 6   MEPGDIAIFKNFTSICTYADRIRCTGVFTGANPLEFSVPLLLLQLGVSAGTIVLFAWLLK 65
           ME  D +IF NF+SIC + DRI  + VF G NPLEFSVPLLLLQLG+ +GTI+    LLK
Sbjct: 5   MESDDSSIFNNFSSICVHVDRIHSSSVFIGENPLEFSVPLLLLQLGICSGTIIFLFQLLK 64

Query: 66  PLGQPLIVSQILVGLVLGSSGLSHLENFRETIFPLRGFICLDAVSALGQIFYFFLIGVQT 125
            L QPLIVSQIL GLVLG  G   LE FRETIFP+RG I LDA+S+LGQ+FYFFLIGVQT
Sbjct: 65  RLAQPLIVSQILSGLVLGYFGAGFLEKFRETIFPIRGLIFLDAISSLGQVFYFFLIGVQT 124

Query: 126 DISIMKNIDRRAFGIGFCSVILPMILITIYPIILVNIVDFKFVKSLFVLGKIETFINFPM 185
           D+SI+KNID+RAFGIG CSVILP IL  +Y + LVN VD   +K+LF++G  E+FI+F M
Sbjct: 125 DMSIIKNIDKRAFGIGVCSVILPTILSNLYTVTLVNTVDLDTIKTLFLVGNAESFIHFAM 184

Query: 186 VASLLSELHLINSEFGRIALSSSMVSNFLSIFFLLIGAILFPQSVTGSKILSTTYASAVV 245
           VASLL+ELHLINS+FG+IAL+SSM SN   +FF  IG +L  +       LST Y   +V
Sbjct: 185 VASLLNELHLINSKFGKIALASSMSSNCFGLFFQKIGMLLANERKVRLHALSTVY-GFIV 244

Query: 246 VVVIFFAIRPIVLWMVKKNPIGQQLKEGFVITLLLGVLVVAFCSQASGLHIYFGPLLLGI 305
           + VI  + +P++LWM K +P+GQ LKE FVITL LGVLVVAFCSQASG+HIYFGP+LLGI
Sbjct: 245 LAVIILSFQPVILWMTKNSPVGQPLKESFVITLFLGVLVVAFCSQASGMHIYFGPILLGI 304

Query: 306 TIPPGPPIGSALVERLDLITSWVFMPVFFVKIGLVVNIYTIKLKNFLCISFIILVGALGK 365
           TIPPGPPIGSA+VE+LD+I SWV MP+FF KIGLV +I+TIKL NFLCISFII+V A GK
Sbjct: 305 TIPPGPPIGSAMVEKLDIIASWVLMPIFFAKIGLVFSIHTIKLLNFLCISFIIIVAAFGK 364

Query: 366 FLGALVISLYYKLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVINDEAFVVMCICVMVL 425
           FLGAL++S+YYKLP R GV+LGL+LNSQGALE+GI+KLM+K+KV+++E FVVMCICVMV+
Sbjct: 365 FLGALMMSIYYKLPTRDGVALGLILNSQGALEIGIYKLMKKDKVLDEETFVVMCICVMVV 424

Query: 426 VAVITPIIGHLFDPSRRYVVHKKRTVMHSRPEIDLCVLVCIHDQEDVPSAINLLEALNPT 485
            AVITPII +LFDPSRR+VV KKRTVMH RPE DL VLVCIHDQ+DVPSAIN+LEAL P 
Sbjct: 425 AAVITPIIRYLFDPSRRFVVEKKRTVMHLRPEFDLSVLVCIHDQDDVPSAINILEALKPM 484

Query: 486 RRSHLIVYMLHLVELLGRANPQLIHHKLTKIRT-SRSCPSEHIVNAFKHFGQSNREIVAI 545
            R+HLIVYMLHLV LLGRANPQLIHH++TK+RT SRSC SE IVNAFK+FGQSN ++V I
Sbjct: 485 HRNHLIVYMLHLVVLLGRANPQLIHHRITKLRTSSRSCVSESIVNAFKYFGQSNSDVVTI 544

Query: 546 HPFTAISPSATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSKSKMKMVNHHIFDKA 605
           HPFTAISPS TMY+DVCSLALDKR+SLIL+PFHKRFHSNG+MSLSK KMKM N HI DKA
Sbjct: 545 HPFTAISPSTTMYEDVCSLALDKRTSLILIPFHKRFHSNGMMSLSKYKMKMFNQHILDKA 604

Query: 606 PCSVAVVVERGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGARMAGHSNINLTVIR 665
           PCSVA++VERGI  ++RSI+TNLYSFQ+A VF+GGPDDREAIFIG RM GH  IN+T+IR
Sbjct: 605 PCSVALIVERGIVKISRSISTNLYSFQIAIVFIGGPDDREAIFIGTRMIGHPYINVTLIR 664

Query: 666 LLEDGNVSSDDVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDGTGVVSVLRTMGNNFD 725
           LLED N  SDD KE +L+NEAV ++ +++ NN++VRY+EEV KD TG V++LR++ NNFD
Sbjct: 665 LLEDTNSPSDDGKEMKLNNEAVSKYHRLLENNHRVRYMEEVAKDCTGTVAILRSLENNFD 724

Query: 726 LVMVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMILVVQQHTKVVNEEEE 785
           L+MVGRRHS    LVQGL+ WNEQTELG+IGEVLASSDF+GN  I VVQQH KVVNE+ E
Sbjct: 725 LIMVGRRHSPRSPLVQGLLLWNEQTELGSIGEVLASSDFIGNATIFVVQQHRKVVNEDPE 783

BLAST of Tan0022359 vs. ExPASy TrEMBL
Match: A0A1S3BNS6 (cation/H(+) antiporter 15-like OS=Cucumis melo OX=3656 GN=LOC103491876 PE=4 SV=1)

HSP 1 Score: 1062.0 bits (2745), Expect = 1.2e-306
Identity = 545/796 (68.47%), Postives = 655/796 (82.29%), Query Frame = 0

Query: 1   MGSIVMEPGDIAIF----------KNFTSICTYADRIRCTGVFTGANPLEFSVPLLLLQL 60
           MGSIVMEP DIA +          KNFT+ICT+A+ I CT +F GANPLEFSVPLL LQL
Sbjct: 1   MGSIVMEPNDIATYINGGIGHRPTKNFTTICTFANHIHCTSIFIGANPLEFSVPLLFLQL 60

Query: 61  GVSAGTIVLFAWLLKPLGQPLIVSQILVGLVLGSSGLSHLENFRETIFPLRGFICLDAVS 120
           G+ +GTI+LF+ LLKPLGQPLIVSQIL GLVLGS+GLS++E F+ETIFPLRGF+ LD VS
Sbjct: 61  GICSGTIILFSQLLKPLGQPLIVSQILGGLVLGSTGLSNVEIFKETIFPLRGFVSLDVVS 120

Query: 121 ALGQIFYFFLIGVQTDISIMKNIDRRAFGIGFCSVILPMILITIYPIILVNIVDFKFVKS 180
           ALG IFY+FLIGVQTDISI+KNID + FGIG C+ IL  +L+ IY + L +I+D    K 
Sbjct: 121 ALGHIFYYFLIGVQTDISIVKNIDMKTFGIGSCATILATLLVIIYSMSLASIIDMTKFKY 180

Query: 181 LFVLGKIETFINFPMVASLLSELHLINSEFGRIALSSSMVSNFLSIFFLLIGAILFPQSV 240
           +F LGK+E+FINFPMVASL+ ELHL+NSEFGRI+L+SSM S+ LS+   L+G+IL P   
Sbjct: 181 IFELGKLESFINFPMVASLVYELHLVNSEFGRISLTSSMASSLLSVSLTLLGSILSPNGT 240

Query: 241 TGSKILSTTYASAVVVVVIFFAIRPIVLWMVKKNPIGQQLKEGFVITLLLGVLVVAFCSQ 300
           T  +ILS T+A  V+++VI F+IRP  LWMVK NPIGQ LKE FVITLLLGVLVVAFC Q
Sbjct: 241 TRQQILSETFAIMVLILVIVFSIRPATLWMVKMNPIGQPLKECFVITLLLGVLVVAFCCQ 300

Query: 301 ASGLHIYFGPLLLGITIPPGPPIGSALVERLDLITSWVFMPVFFVKIGLVVNIYTIKLKN 360
           A GL IYF    +G  IP GPPIGS LV+RLD IT+W+FMPVFF +IGL V+IY  KL N
Sbjct: 301 ALGLRIYFASFFIGFIIPSGPPIGSTLVDRLDFITTWIFMPVFFARIGLSVDIYNTKLIN 360

Query: 361 FLCISFIILVGALGKFLGALVISLYYKLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVI 420
           F+C+SFI++V ALGKFLGAL+IS+YYKLP+R  +SLGL+LNSQGALEL  F++ +++KVI
Sbjct: 361 FICMSFIVIVSALGKFLGALMISMYYKLPMRDAISLGLILNSQGALELMTFRMKKRDKVI 420

Query: 421 NDEAFVVMCICVMVLVAVITPIIGHLFDPSRRYVVHKKRTVMHSRPEIDLCVLVCIHDQE 480
           +D+AFVV C+ + V+VA+ITP I +L  PSRRY+V K+RTVMH+RPE DLCVLVCIHDQE
Sbjct: 421 DDDAFVVGCLYITVMVAIITPAIRYLLHPSRRYIVKKRRTVMHTRPEHDLCVLVCIHDQE 480

Query: 481 DVPSAINLLEAL-NPTRRSHLIVYMLHLVELLGRANPQLIHHKLTKIRTSRSCPSEHIVN 540
           DVPSAINLL+AL NP ++S L+VYMLHLVELLG A P+LIHH+ TK++TSRS  SE IVN
Sbjct: 481 DVPSAINLLDALNNPMKQSQLVVYMLHLVELLGHAQPKLIHHRFTKVKTSRSYSSEPIVN 540

Query: 541 AFKHFGQSNREIVAIHPFTAISPSATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLS 600
           AFK+FG SN EIV I+PFTAISP  TM+DDVCSLALDK+S LI VPFHKRFHSNGVMS S
Sbjct: 541 AFKYFGDSNNEIVVINPFTAISPFTTMHDDVCSLALDKKSFLIFVPFHKRFHSNGVMSSS 600

Query: 601 KSKMKMVNHHIFDKAPCSVAVVVERGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIG 660
           K K+KMVN +I + APCSVA+VVERG   +++SI T LYSFQ+A VF+GG DDREA+FIG
Sbjct: 601 KYKLKMVNDNILENAPCSVALVVERGFLKLSKSIETCLYSFQIAVVFIGGADDREAMFIG 660

Query: 661 ARMAGHSNINLTVIRLLEDGNVSSDDVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDG 720
           ARMAGH+NINLT+IR+LE   V SD+ +E R+D+EAV EFR+M  +NY+VRYIEEVVKDG
Sbjct: 661 ARMAGHANINLTMIRVLESEKVGSDEAEESRVDDEAVDEFRRMTVDNYRVRYIEEVVKDG 720

Query: 721 TGVVSVLRTMGNNFDLVMVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMI 780
            G + +LR+MG+NFDLVMVGRRHS C ALVQGLV WNE TELGAIGEVLA+SDFMGN MI
Sbjct: 721 IGTICILRSMGSNFDLVMVGRRHSPCSALVQGLVLWNEHTELGAIGEVLATSDFMGNAMI 780

Query: 781 LVVQQHTKVVNEEEEN 786
           LV+QQHT+V NE  +N
Sbjct: 781 LVIQQHTRVANENHDN 796

BLAST of Tan0022359 vs. TAIR 10
Match: AT2G13620.1 (cation/hydrogen exchanger 15 )

HSP 1 Score: 508.1 bits (1307), Expect = 1.3e-143
Identity = 292/791 (36.92%), Postives = 466/791 (58.91%), Query Frame = 0

Query: 20  ICTYADRIRCTGVFTGANPLEFSVPLLLLQLGVSAGTIVLFAWLLKPLGQPLIVSQILVG 79
           IC     I   GV+ G NPL+FS+PL +LQL +       F ++LKP  QP ++S+IL G
Sbjct: 14  ICYAPSMITTNGVWQGDNPLDFSLPLFVLQLTLVVVVTRFFVFILKPFRQPRVISEILGG 73

Query: 80  LVLGSSGLSHLENFRETIFPLRGFICLDAVSALGQIFYFFLIGVQTDISIMKNIDRRAFG 139
           +VLG S L     F  TIFP R  + L+ ++ +G +++ FL+GV+ DI +++   +RA  
Sbjct: 74  IVLGPSVLGRSTKFAHTIFPQRSVMVLETMANVGLLYFLFLVGVEMDIMVVRKTGKRALT 133

Query: 140 IGFCSVILPMILITIYPIILVNIVDFKFVKS-LFVLGKIETFINFPMVASLLSELHLINS 199
           I    ++LP ++   +   +    D     + +  LG   +   FP++A +L+EL LIN+
Sbjct: 134 IAIGGMVLPFLIGAAFSFSMHRSEDHLGQGTYILFLGVALSVTAFPVLARILAELKLINT 193

Query: 200 EFGRIALSSSMVSNFLSIFFLLIGAILFPQSVTGSKILSTTYASAVVVVVIFFAIRPIVL 259
           E GRI++S+++V++  +   L +   L     T    L    +SAV + V  F +RP + 
Sbjct: 194 EIGRISMSAALVNDMFAWILLALAIALAESDKTSFASLWVMISSAVFIAVCVFVVRPGIA 253

Query: 260 WMVKKNPIGQQLKEGFVITLLLGVLVVAFCSQASGLHIYFGPLLLGITIPPGPPIGSALV 319
           W+++K P G+   E  +  +L GV++  F + A G H  FG  + G+ IP G P+G  L+
Sbjct: 254 WIIRKTPEGENFSEFHICLILTGVMISGFITDAIGTHSVFGAFVFGLVIPNG-PLGLTLI 313

Query: 320 ERLDLITSWVFMPVFFVKIGLVVNIYTIK-LKNFLCISFIILVGALGKFLGALVISLYYK 379
           E+L+   S + +P+FF   GL  NI  I+    +L +  +I +   GK +G ++++ ++ 
Sbjct: 314 EKLEDFVSGLLLPLFFAISGLKTNIAAIQGPATWLTLFLVIFLACAGKVIGTVIVAFFHG 373

Query: 380 LPVRHGVSLGLVLNSQGALELGIFKLMRKNKVINDEAFVVMCICVMVLVAVITPIIGHLF 439
           +PVR G++LGL+LN++G +E+ +  + +  KV++DE F  M +  +V+  VITPI+  L+
Sbjct: 374 MPVREGITLGLLLNTKGLVEMIVLNVGKDQKVLDDETFATMVLVALVMTGVITPIVTILY 433

Query: 440 DPSRRYVVHKKRTVMHSRPEIDLCVLVCIHDQEDVPSAINLLEALNPTRRSHLIVYMLHL 499
            P ++ V +K+RT+  ++P+ +L VLVC+H   +VP+ INLLEA +PT+RS + +Y+LHL
Sbjct: 434 KPVKKSVSYKRRTIQQTKPDSELRVLVCVHTPRNVPTIINLLEASHPTKRSPICIYVLHL 493

Query: 500 VELLGRANPQLIHHKLTK----IRTSRSCPSEHIVNAFKHFGQSNREIVAIHPFTAISPS 559
           VEL GRA+  LI H   K            S+HI+NAF+++ Q +   VA+ P TAISP 
Sbjct: 494 VELTGRASAMLIVHNTRKSGRPALNRTQAQSDHIINAFENYEQ-HAAFVAVQPLTAISPY 553

Query: 560 ATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSKSKMKMVNHHIFDKAPCSVAVVVE 619
           +TM++DVCSLA DKR S I++PFHK+   +G M  +    ++VN ++ + +PCSV ++V+
Sbjct: 554 STMHEDVCSLAEDKRVSFIIIPFHKQQTVDGGMESTNPAYRLVNQNLLENSPCSVGILVD 613

Query: 620 RGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGARMAGHSNINLTVIRLLEDGNVSS 679
           RG+   TR + +N  S QVA +F GGPDDREA+    RMA H  I LTV+R + D + + 
Sbjct: 614 RGLNGATR-LNSNTVSLQVAVLFFGGPDDREALAYAWRMAQHPGITLTVLRFIHDEDEAD 673

Query: 680 -----------------DDVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDGTGVVSVL 739
                            D  K+R+LD++ +  FR   A    + YIE++V +G   V+ +
Sbjct: 674 TASTRATNDSDLKIPKMDHRKQRQLDDDYINLFRAENAEYESIVYIEKLVSNGEETVAAV 733

Query: 740 RTMGNNFDLVMVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMILVVQQHT 788
           R+M ++ DL +VGR       L  GL  W+E  ELGAIG++LASSDF     +LVVQQ+ 
Sbjct: 734 RSMDSSHDLFIVGRGEGMSSPLTAGLTDWSECPELGAIGDLLASSDFAATVSVLVVQQYV 793

BLAST of Tan0022359 vs. TAIR 10
Match: AT1G06970.1 (cation/hydrogen exchanger 14 )

HSP 1 Score: 412.1 bits (1058), Expect = 9.8e-115
Identity = 251/785 (31.97%), Postives = 442/785 (56.31%), Query Frame = 0

Query: 20  ICTYADRIRCTGVFTGANPLEFSVPLLLLQLGVSAGTIVLFAWLLKPLGQPLIVSQILVG 79
           +C     +   GVF G++PL++++PL+LLQ+ V   T  L   LLKPL Q +I +Q+L G
Sbjct: 27  VCQKNHMLTSKGVFLGSDPLKYAMPLMLLQMSVIIITSRLLYRLLKPLKQGMISAQVLAG 86

Query: 80  LVLGSSGLSHLENFRETIFPLRGFICLDAVSALGQIFYFFLIGVQTDISIMKNIDRRAFG 139
           ++LG S       + +   P+ G I L  +S LG   + FL+G++ D SI++    +A  
Sbjct: 87  IILGPSLFGQSSAYMQMFLPISGKITLQTLSNLGFFIHLFLLGLRIDASIIRKAGSKAIL 146

Query: 140 IGFCSVILPMILITIYPIILVNIVDF--KFVKSLFVLGKIETFINFPMVASLLSELHLIN 199
           IG  S  LP  L  +  + L N  +     V  +  +  +    +FP+  ++L+EL+++N
Sbjct: 147 IGTASYALPFSLGNLTVLFLKNTYNLPPDVVHCISTVISLNAMTSFPVTTTVLAELNILN 206

Query: 200 SEFGRIALSSSMVSNFLSIFFLLIGAILFPQSVTGSKILSTTYASAVVVVVIFFAIRPIV 259
           S+ GR+A + S+V    S    L+   +F +  T + + S  + +A +++VIFF  RP +
Sbjct: 207 SDLGRLATNCSIVCEAFSWIVALVFR-MFLRDGTLASVWSFVWVTA-LILVIFFVCRPAI 266

Query: 260 LWMVKKNPIG-QQLKEGFVITLLLGVLVVAFCSQASGLHIYFGPLLLGITIPPGPPIGSA 319
           +W+ ++  I   +  E     +++ +L ++  S+  G+H  FG   LG+++P GPP+G+ 
Sbjct: 267 IWLTERRSISIDKAGEIPFFPIIMVLLTISLTSEVLGVHAAFGAFWLGVSLPDGPPLGTG 326

Query: 320 LVERLDLITSWVFMPVFFVKIGLVVNIYTIKLKNFLCISFIILVGALGKFLGALVISLYY 379
           L  +L++  + + +P F    GL  N + I   +   I  +IL+    KFLG    S Y 
Sbjct: 327 LTTKLEMFATSLMLPCFISISGLQTNFFIIGESHVKIIEAVILITYGCKFLGTAAASAYC 386

Query: 380 KLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVINDEAFVVMCICVMVLVAVITPIIGHL 439
            + +    SL L++  QG +E+    + +  KV+N E F ++ I ++++  +   ++  L
Sbjct: 387 NIQIGDAFSLALLMCCQGVIEIYTCVMWKDEKVLNTECFNLLIITLLLVTGISRFLVVCL 446

Query: 440 FDPSRRYVVHKKRTVMHSRP-EIDLCVLVCIHDQEDVPSAINLLEALNPTRRSHLIVYML 499
           +DPS+RY    KRT++ +R   +   +L+C+++ E+VPS +NLLEA  P+R S + V+ L
Sbjct: 447 YDPSKRYRSKSKRTILDTRQRNLQFRLLLCVYNVENVPSMVNLLEASYPSRFSPISVFTL 506

Query: 500 HLVELLGRANPQLI-HHKLTKIRTSRSCPSEHIVNAFKHFGQSNREIVAIHPFTAISPSA 559
           HLVEL GRA+  L+ HH++ K+    +  S HIVN F+ F Q N+  +    FTA +P +
Sbjct: 507 HLVELKGRAHAVLVPHHQMNKL-DPNTVQSTHIVNGFQRFEQQNQGTLMAQHFTAAAPFS 566

Query: 560 TMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSKSKMKMVNHHIFDKAPCSVAVVVER 619
           ++ DD+C+LALDK+++LI++PFHK++  +G +      ++ +N ++ +KAPCSV + ++R
Sbjct: 567 SINDDICTLALDKKATLIVIPFHKQYAIDGTVDHVNPSIRNINLNVLEKAPCSVGIFIDR 626

Query: 620 GIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGARMAGHSNINLTVIRLLEDGNVSSD 679
           G     RS+  +     VA +F+ G DD EA+    R+A H  +++T+I      ++  +
Sbjct: 627 GETEGRRSVLMSYTWRNVAVIFIEGRDDAEALAFSMRIAEHPEVSVTMIHFRHKSSLQQN 686

Query: 680 ---DVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDGTGVVSVLRTMGNNFDLVMVGRR 739
              DV+    ++  + +F+    +  K+ Y EE+V+DG     V+ ++G++FDLV+VGR 
Sbjct: 687 HVVDVESELAESYLINDFKNFAMSKPKISYREEIVRDGVETTQVISSLGDSFDLVVVGRD 746

Query: 740 HSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMILVVQQHTKVVNEEEENHFGTNI 797
           H    +++ GL  W+E  ELG IG++ ASSDF  +  +LV+ Q             G ++
Sbjct: 747 HDLESSVLYGLTDWSECPELGVIGDMFASSDF--HFSVLVIHQQE-----------GDSL 795

BLAST of Tan0022359 vs. TAIR 10
Match: AT2G30240.1 (Cation/hydrogen exchanger family protein )

HSP 1 Score: 393.7 bits (1010), Expect = 3.6e-109
Identity = 245/768 (31.90%), Postives = 431/768 (56.12%), Query Frame = 0

Query: 20  ICTYADRIRCTGVFTGANPLEFSVPLLLLQLGVSAGTIVLFAWLLKPLGQPLIVSQILVG 79
           +C   + +   G+F  +NPL++++PLLLLQ+ V   T  L   +L+PL Q +I +Q+L G
Sbjct: 29  VCQAQNMLTSRGIFMKSNPLKYALPLLLLQMSVIIVTSRLIFRVLQPLKQGMISAQVLTG 88

Query: 80  LVLGSSGLSHLENFRETIFPLRGFICLDAVSALGQIFYFFLIGVQTDISIMKNIDRRAFG 139
           +VLG S L H   +     P  G I +  +S +G + + FL+G++ D SI++    +A  
Sbjct: 89  VVLGPSFLGHNVIYMNMFLPAGGKIIIQTLSNVGFVIHLFLLGLKIDGSIIRKAGSKAIL 148

Query: 140 IGFCSVILPMIL--ITIYPIILVNIVDFKFVKSLFVLGKIETFINFPMVASLLSELHLIN 199
           IG  S   P  L  +TI  I     +    +        + +  +FP+  ++L+EL+++N
Sbjct: 149 IGTASYAFPFSLGNLTIMFISKTMGLPSDVISCTSSAISLSSMTSFPVTTTVLAELNILN 208

Query: 200 SEFGRIALSSSMVSNFLSIFFLLIGAILFPQSVTGSKILSTTYASAVVV---VVIFFAIR 259
           SE GR+A   SMV    S F  L   +      T  + +++ YA ++++   +VI+F  R
Sbjct: 209 SELGRLATHCSMVCEVCSWFVALAFNL-----YTRDRTMTSLYALSMIIGLLLVIYFVFR 268

Query: 260 PIVLWMVKKNPIGQQLKE---GFVITLLLGVLVVAFCSQASGLHIYFGPLLLGITIPPGP 319
           PI++W+ ++       K+    F + LLL   + +   +A G+H  FG   LG+++P GP
Sbjct: 269 PIIVWLTQRKTKSMDKKDVVPFFPVLLLLS--IASLSGEAMGVHAAFGAFWLGVSLPDGP 328

Query: 320 PIGSALVERLDLITSWVFMPVFFVKIGLVVNIYTIKLK---NFLCISFIILVGALGKFLG 379
           P+G+ L  +L++  S +F+P F    GL  N + I      + + I  I+L+    KFLG
Sbjct: 329 PLGTELAAKLEMFASNLFLPCFIAISGLQTNFFEITESHEHHVVMIEIILLITYGCKFLG 388

Query: 380 ALVISLYYKLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVINDEAFVVMCICVMVLVAV 439
               S Y +  +   + L  ++  QG +E+    + +  +V++ E F ++ I ++ +  +
Sbjct: 389 TAAASAYCQTQIGDALCLAFLMCCQGIIEVYTTIVWKDAQVVDTECFNLVIITILFVTGI 448

Query: 440 ITPIIGHLFDPSRRYVVHKKRTVMHSRP-EIDLCVLVCIHDQEDVPSAINLLEALNPTRR 499
              ++ +L+DPS+RY    KRT++++R   + L +L+ +++ E+VPS +NLLEA  PTR 
Sbjct: 449 SRFLVVYLYDPSKRYKSKSKRTILNTRQHNLQLRLLLGLYNVENVPSMVNLLEATYPTRF 508

Query: 500 SHLIVYMLHLVELLGRANPQLI-HHKLTKIRTSRSCPSEHIVNAFKHFGQSNREIVAIHP 559
           + +  + LHLVEL GRA+  L  HH++ K+    +  S HIVNAF+ F Q  +  +    
Sbjct: 509 NPISFFTLHLVELKGRAHALLTPHHQMNKL-DPNTAQSTHIVNAFQRFEQKYQGALMAQH 568

Query: 560 FTAISPSATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSKSKMKMVNHHIFDKAPC 619
           FTA +P +++ +D+C+LALDK+++LI++PFHK++  +G +      ++ +N ++ D APC
Sbjct: 569 FTAAAPYSSINNDICTLALDKKATLIVIPFHKQYAIDGTVGQVNGPIRTINLNVLDAAPC 628

Query: 620 SVAVVVERGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGARMAGHSNINLTVIRLL 679
           SVA+ ++RG     RS+        VA +F+GG DD EA+ +  RMA   ++N+T+I   
Sbjct: 629 SVAIFIDRGETEGRRSVLMTNTWQNVAMLFIGGKDDAEALALCMRMAEKPDLNVTMIHFR 688

Query: 680 EDGNVSSDDVKERRLDNEAVVEFRQMMANNYKVRYIEEVVKDGTGVVSVLRTMGNNFDLV 739
               +  +D  +   +   + +F+   AN  K+ Y+EE+V+DG     V+ ++G+ +D+V
Sbjct: 689 HKSALQDEDYSDMS-EYNLISDFKSYAANKGKIHYVEEIVRDGVETTQVISSLGDAYDMV 748

Query: 740 MVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMILVVQQ 775
           +VGR H    +++ GL  W+E  ELG IG++L S DF  + +++  QQ
Sbjct: 749 LVGRDHDLESSVLYGLTDWSECPELGVIGDMLTSPDFHFSVLVVHQQQ 787

BLAST of Tan0022359 vs. TAIR 10
Match: AT5G41610.1 (cation/H+ exchanger 18 )

HSP 1 Score: 367.1 bits (941), Expect = 3.6e-101
Identity = 242/789 (30.67%), Postives = 423/789 (53.61%), Query Frame = 0

Query: 16  NFTSICTYADRIRCTGVFTGANPLEFSVPLLLLQLGVSAGTIVLFAWLLKPLGQPLIVSQ 75
           N T  C    +    GVF G NP++F++PL +LQ+ +      + A+LL+PL QP ++++
Sbjct: 4   NSTKACPAPMKATSNGVFQGDNPIDFALPLAILQIVIVIVLTRVLAYLLRPLRQPRVIAE 63

Query: 76  ILVGLVLGSSGLSHLENFRETIFPLRGFICLDAVSALGQIFYFFLIGVQTDISIMKNIDR 135
           ++ G++LG S L   + F + +FP +    L+ ++ LG +F+ FL G++ D   ++   +
Sbjct: 64  VIGGIMLGPSLLGRSKAFLDAVFPKKSLTVLETLANLGLLFFLFLAGLEIDTKALRRTGK 123

Query: 136 RAFGIGFCSVILPMILITIYPIILVNIVDFKFVKS---LFVLGKIETFINFPMVASLLSE 195
           +A GI    + LP  L      +L   +  K V S   L  +G   +   FP++A +L+E
Sbjct: 124 KALGIALAGITLPFALGIGSSFVLKATIS-KGVNSTAFLVFMGVALSITAFPVLARILAE 183

Query: 196 LHLINSEFGRIALSSSMVSNFLSIFFLLIGAILFPQSVTGSKILSTTYASAVVVVV-IFF 255
           L L+ +E GR+A+S++ V N ++ + LL  AI    S T   +    + S    V+   F
Sbjct: 184 LKLLTTEIGRLAMSAAAV-NDVAAWILLALAIALSGSNTSPLVSLWVFLSGCAFVIGASF 243

Query: 256 AIRPIVLWMVKKNPIGQQLKEGFVITLLLGVLVVAFCSQASGLHIYFGPLLLGITIPPGP 315
            I PI  W+ ++   G+ ++E ++   L  VLV  F + A G+H  FG  ++G+ IP   
Sbjct: 244 IIPPIFRWISRRCHEGEPIEETYICATLAVVLVCGFITDAIGIHSMFGAFVVGVLIPKEG 303

Query: 316 PIGSALVERLDLITSWVFMPVFFVKIGLVVNIYTIK-LKNFLCISFIILVGALGKFLGAL 375
           P   ALVE+++ + S +F+P++FV  GL  N+ TI+  +++  +  +      GK LG L
Sbjct: 304 PFAGALVEKVEDLVSGLFLPLYFVASGLKTNVATIQGAQSWGLLVLVTATACFGKILGTL 363

Query: 376 VISLYYKLPVRHGVSLGLVLNSQGALELGIFKLMRKNKVINDEAFVVMCICVMVLVAVIT 435
            +SL +K+P+R  ++LG ++N++G +EL +  + +  KV+ND+ F +M +  +    + T
Sbjct: 364 GVSLAFKIPMREAITLGFLMNTKGLVELIVLNIGKDRKVLNDQTFAIMVLMALFTTFITT 423

Query: 436 PIIGHLFDPSRRYV---VHKKRTVMHSRPEIDLCVLVCIHDQEDVPSAINLLEALNPTRR 495
           P++  ++ P+RR      +K R V        L +L C H    +PS INLLEA     +
Sbjct: 424 PVVMAVYKPARRAKKEGEYKHRAVERENTNTQLRILTCFHGAGSIPSMINLLEASRGIEK 483

Query: 496 SH-LIVYMLHLVELLGRANPQLIHHKLTK------IRTSRSCPSEHIVNAFKHFGQSNRE 555
              L VY LHL EL  R++  L+ HK+ K       R   +  ++ +V AF+ F Q +R 
Sbjct: 484 GEGLCVYALHLRELSERSSAILMVHKVRKNGMPFWNRRGVNADADQVVVAFQAFQQLSR- 543

Query: 556 IVAIHPFTAISPSATMYDDVCSLALDKRSSLILVPFHKRFHSNGVMSLSKSKMKMVNHHI 615
            V + P TAIS  + +++D+C+ A+ K+++++++PFHK    +G +  ++   + VN  +
Sbjct: 544 -VNVRPMTAISSMSDIHEDICTTAVRKKAAIVILPFHKHQQLDGSLETTRGDYRWVNRRV 603

Query: 616 FDKAPCSVAVVVERGIFNVTRSIATNLYSFQVATVFLGGPDDREAIFIGARMAGHSNINL 675
             +APCSV + V+RG+   ++  A ++ S+ V  +F GGPDDREA+  G RMA H  I L
Sbjct: 604 LLQAPCSVGIFVDRGLGGSSQVSAQDV-SYSVVVLFFGGPDDREALAYGLRMAEHPGIVL 663

Query: 676 TVIRL--------------LEDGNVSSDDVKERRLDNEAVVEFRQMMANNYKVRYIEEVV 735
           TV R               + + N  +  VK  + D E + E R++ + +  V+++E+ +
Sbjct: 664 TVFRFVVSPERVGEIVNVEVSNNNNENQSVKNLKSDEEIMSEIRKISSVDESVKFVEKQI 723

Query: 736 KDGTGVVSVLRTMGNNFDLVMVGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGN 776
           ++    V          +L +VGR     +AL   +   +E  ELG +G +L S +    
Sbjct: 724 ENAAVDVRSAIEEVRRSNLFLVGRMPGGEIAL--AIRENSECPELGPVGSLLISPESSTK 783

BLAST of Tan0022359 vs. TAIR 10
Match: AT5G22900.1 (cation/H+ exchanger 3 )

HSP 1 Score: 360.5 bits (924), Expect = 3.4e-99
Identity = 239/772 (30.96%), Postives = 415/772 (53.76%), Query Frame = 0

Query: 57  IVLFAW-----LLKPLGQPLIVSQILVGLVLGSSGLSH---------LENFRETIFPLRG 116
           I+ F W      L+ LG     S +L G++L  S L            E+++E +F L  
Sbjct: 64  IISFLWQFLHFFLRRLGMIRFTSHMLTGVLLSKSFLKENSAARRFFSTEDYKEIVFSL-- 123

Query: 117 FICLDAVSALGQIFYFFLIGVQTDISIMKNIDRRAFGIGFCSVILPMILITIYPIILVNI 176
                  +A   + ++FL+GV+ D  +++   R+A  IG  SV+L  ++ ++  I   N+
Sbjct: 124 ------TAACSYMMFWFLMGVKMDTGLIRTTGRKAITIGLSSVLLSTLVCSV--IFFGNL 183

Query: 177 VDFKFVKSLFVLGKIETFI--------NFPMVASLLSELHLINSEFGRIALSSSMVSNF- 236
            D     S   L  +E  +        +FP+V +LL EL L NSE GR+A+SS+++S+F 
Sbjct: 184 RDVGTKNSDHTLNSLEYVVIYSIQCLSSFPVVGNLLFELRLQNSELGRLAISSAVISDFS 243

Query: 237 ----LSIFFLL---------IGAILFPQSVTGSKILSTTYASAVVVVVIFFAIRPIVLWM 296
                S+   +         +G++     + G++ L       + V +  +  RP++ ++
Sbjct: 244 TSILASVLIFMKELKDEQTRLGSVFIGDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYI 303

Query: 297 VKKNPIGQQLKEGFVITLLLGVLVVAFCSQASGLHIYFGPLLLGITIPPGPPIGSALVER 356
           +K+ P G+ +K  ++ T+++ V   A  +      I+ GP +LG+ +P GPP+GSA++++
Sbjct: 304 IKQTPSGRPVKAIYLSTIIVMVSGSAILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQK 363

Query: 357 LDLITSWVFMPVFFVKIGLVVNIYTI-KLKNFLCISFIILVGALGKFLGALVISLYYKLP 416
            +      F+P F       ++I  +   +    I  I++   + KF+   V +L+Y +P
Sbjct: 364 YESAIFGTFLPFFIASSSTEIDISALFGWEGLNGIILIMVTSFVVKFIFTTVPALFYGMP 423

Query: 417 VRHGVSLGLVLNSQGALELGIFKLMRKNKVINDEAFVVMCICVMVLVAVITPIIGHLFDP 476
           +    +L L+++ +G  ELG + L  +   +  E F V C+ + +  A+I PI+ +L+DP
Sbjct: 424 MEDCFALSLIMSFKGIFELGAYALAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDP 483

Query: 477 SRRYVVHKKRTVMHSRPEIDLCVLVCIHDQEDVPSAINLLEALNPTRRSHLIVYMLHLVE 536
           SR Y  ++KR + H +P  +L +L CI+  +D+   INLLEA+ P+R S +  Y+LHL+E
Sbjct: 484 SRMYAGYEKRNMQHLKPNSELRILSCIYRTDDISPMINLLEAICPSRESPVATYVLHLME 543

Query: 537 LLGRANPQLIHHKLTKIRTSRSCPSEHIVNAFKHFGQSNREIVAIHPFTAISPSATMYDD 596
           L+G+ANP  I HKL   RT  +  S +++ +F+ F +     V +  +TA+S   TM+ D
Sbjct: 544 LVGQANPIFISHKLQTRRTEETSYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGD 603

Query: 597 VCSLALDKRSSLILVPFHKRFHSNGVMSLSKSKM-KMVNHHIFDKAPCSVAVVVER---G 656
           +C LAL+  +SLIL+PFH+ + ++G   +S + M + +N  + D APCSV V V R   G
Sbjct: 604 ICMLALNNTTSLILLPFHQTWSADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSG 663

Query: 657 IFNVTRSIAT------NLYSFQVATVFLGGPDDREAIFIGARMAGHSNINLTVIRLL-ED 716
             N++    T      NL S+ +  +FLGG DDREA+ +  RMA    IN+T++RL+  D
Sbjct: 664 RKNISSGRKTINGTVPNLSSYNICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTD 723

Query: 717 GNVSSDDVKERRLDNEAVVEFRQMMANN-YKVRYIEEVVKDGTGVVSVLRTMGNNFDLVM 776
                + V ++ LD+E +   R + +N    + Y E+ ++D     S+LR+M ++FD+ +
Sbjct: 724 EKARENTVWDKMLDDELL---RDVKSNTLVDIFYSEKAIEDAAETSSLLRSMVSDFDMFI 783

Query: 777 VGRRHSSCLALVQGLVFWNEQTELGAIGEVLASSDFMGNTMILVVQQHTKVV 780
           VGR +       +GL  W+E  ELG IG++L S DF     +LV+QQ   ++
Sbjct: 784 VGRGNGRTSVFTEGLEEWSEFKELGIIGDLLTSQDFNCQASVLVIQQQQLMI 822