Tan0021862 (gene) Snake gourd v1

Overview


Name	Tan0021862
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	phospholipase A2-alpha
Location	LG09: 69155262 .. 69157659 (-)
RNA-Seq Expression	Tan0021862
Synteny	Tan0021862

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTTCCAAAAAACCCATATCACGAGCCACTACAAGACAACCCTTTTTTCCTCTTTCTTTACCCAAAAATCGCAATTTATTTTCAAGTTTTAAACAAAATTCAAAGCCTTTCTGAGAAAAACGAGAGCGACGGACGAGCCACCAAAAAAGAAAAGAAAAAAATGTTACTTCTTCTTCTTCTTCATGTTCTCTGCCTGGCCATTGTTTCAGCGCCCACTCAGGCACTCAACATTGGCGTTCAAGCCCTCGATGCCTCAATCACATTGGTTCTTCTTCTTCTTCCAAATTTAAAACCCACACTTTTAATTCACTCTTTCTTCTTCTTGTTTTTTAGATCTTTGGTTCATAATTTGGATCTGTGTGTGTGTTGTTGGCTGTTTCTTGATTTTGTAGAGCAAAGATTGCAGTAGAAAATGCGAGTCTGAGTTTTGTTCAGGTGGTTCTTTTCTAATTAATAATCTCTCGATATTCTATTCTTTTTTTTTTTAATTAAATTAATTTTGGAATTTGGTTGCAGTGCCTCCACTTTTGAGATATGGGAAATATTGTGGGCTGTTGTATAGCGGCTGCCCTGGGGAACGGCCCTGCGATGGCTTGGATGCTTGCTGTATGAAGCACGATGCCTGTGTTGTTGCTAAAAATGGTCCGTTCTTTCTTCCCCTTTCTATTCATTTTTCTCAACCCAATCATGCTCGGTAAAATCGATAAATTATTCAATGAGATTGTCAAAATATACCCCGAATTTGTACAAGTGATTACTAGAGATATGTAGATTTTTTAATGTACATCCGACGGTTAAAATATTGTGGCTTCTTTGAATAATTTTTCACACACATGAGTCTAGGTAGAAATTATTTGCCAGCTAAAATGATGTGTGGTTTACAAATTTATATAGTTTGGATGTGTGAGAAAGAATTTTAAAGGATGTGTTTTGGAGTTGTAGCTACTAGGTGTGGATAGATTTTGGACCTGTATACAATGGTTATTATTACCCGCATCATATTGTGACATTCTCATTAAATAATTTTTTTGGGCTAGTGGGAGGTTTTTTTTCTTCATCTTGGTCTATTTTTAGTGGTCTATTTGTATTTTTTTCAAAAAATAAGTTTGGATCTCATTAATACATTTAACATAATATCTATTATTATTGTGTAGCTAAGTATGGTTCCTTTCAAACATGCTTTTTATACTAAAATTACTTACCATTTTGTAACTTGCTTTGATTTAGCTTAAATTATTAAAGTTGTCGGTATATCATTGATGTGACATGTTTATTGAATAAATTCTTGTGTAAATTAATACGATGTGGTAAATAAAGTACTAGATATAGATAATGAACATGTCGCATATGTCGCATAGGAGTATTTTGTTAGTTAGCGGTAGAGATTGTATGGGAGTAGGATTTAGAAATTAAGGATGCTTTTAAAAGTTTTGATAATCTATGGGAATGAAGAGATATAGTGATTGAAGTTAGGAAATAAATTTGTAATTTAATCTTGATTTAGACTTAATAATAATTTGTGTGTTGTTTTGTCCAATCAATAATTTTGAAGTTAGGCTGTTTGATGAGAAGGCTAAATTGTCTCTTTGTTTGAGTTAAACCTCACATGTAGGGTGGCTAAGATGGCCCATACATAGCTTAACTTTATATGATTAATTAAATGAAGTCTATTTATTAAAAGAAAACAGGTAATTCCAGGCTATAATTTAATTTCATTCTCAATTGTTTATGTTCAATAGAATTAAGTGAACCAATAAACCTTACATTTATTAGGGACTTGGGAGGATTTTCATACCCAACTCAATTTTTGTTTTTTCATCTATTTTAAAATTTTCATTTTGGCTCTAAATTTTTAAAAGACCTAATGTGGTTCTATCATTACGTTGTATAATCTCATAATTTTAGAGGAAGAAATCACACATCAGTCTTTTACGACTACAAAAATTAGTTTTTAGAAAAGAACGGTCAGTTAATCGAACATGTTATCAGTAAAATTTTGTGTCTACATATATAAAATTGGTGGGTGTGTTTGTGTTTTGTAGATGACTATTTAAGCGAAGAGTGTAGCGAAAACTTCTTGAATTGCATGGAAAACTTCAAGAGATCAAAATCAGCTCCCTTCAAAGGAAACAAATGCCAAGTTAATGAAGTCATGGATGTCATCTCCTTTGTTATGGAAGCTGCCCTTATTGCAGGCAGAGTTTTTCACAACCCCTAGCAAAATTTTCTCCTTTCTATCTTTTTCTTTTTACTGTTTTTTTTTTTTTTCCTTCTATCTAACTCATCCATTAATTTTCTCTAATAGTTGTGATTTGGCATACAACATACTAATGCCCACTTTATTTTCCTTTCTTTTCTCTTTAAACAAAATATGAAATCTCCTCTCTCTCTTAATCAC

mRNA sequence

GTTTCCAAAAAACCCATATCACGAGCCACTACAAGACAACCCTTTTTTCCTCTTTCTTTACCCAAAAATCGCAATTTATTTTCAAGTTTTAAACAAAATTCAAAGCCTTTCTGAGAAAAACGAGAGCGACGGACGAGCCACCAAAAAAGAAAAGAAAAAAATGTTACTTCTTCTTCTTCTTCATGTTCTCTGCCTGGCCATTGTTTCAGCGCCCACTCAGGCACTCAACATTGGCGTTCAAGCCCTCGATGCCTCAATCACATTGAGCAAAGATTGCAGTAGAAAATGCGAGTCTGAGTTTTGTTCAGTGCCTCCACTTTTGAGATATGGGAAATATTGTGGGCTGTTGTATAGCGGCTGCCCTGGGGAACGGCCCTGCGATGGCTTGGATGCTTGCTGTATGAAGCACGATGCCTGTGTTGTTGCTAAAAATGATGACTATTTAAGCGAAGAGTGTAGCGAAAACTTCTTGAATTGCATGGAAAACTTCAAGAGATCAAAATCAGCTCCCTTCAAAGGAAACAAATGCCAAGTTAATGAAGTCATGGATGTCATCTCCTTTGTTATGGAAGCTGCCCTTATTGCAGGCAGAGTTTTTCACAACCCCTAGCAAAATTTTCTCCTTTCTATCTTTTTCTTTTTACTGTTTTTTTTTTTTTTCCTTCTATCTAACTCATCCATTAATTTTCTCTAATAGTTGTGATTTGGCATACAACATACTAATGCCCACTTTATTTTCCTTTCTTTTCTCTTTAAACAAAATATGAAATCTCCTCTCTCTCTTAATCAC

Coding sequence (CDS)

ATGTTACTTCTTCTTCTTCTTCATGTTCTCTGCCTGGCCATTGTTTCAGCGCCCACTCAGGCACTCAACATTGGCGTTCAAGCCCTCGATGCCTCAATCACATTGAGCAAAGATTGCAGTAGAAAATGCGAGTCTGAGTTTTGTTCAGTGCCTCCACTTTTGAGATATGGGAAATATTGTGGGCTGTTGTATAGCGGCTGCCCTGGGGAACGGCCCTGCGATGGCTTGGATGCTTGCTGTATGAAGCACGATGCCTGTGTTGTTGCTAAAAATGATGACTATTTAAGCGAAGAGTGTAGCGAAAACTTCTTGAATTGCATGGAAAACTTCAAGAGATCAAAATCAGCTCCCTTCAAAGGAAACAAATGCCAAGTTAATGAAGTCATGGATGTCATCTCCTTTGTTATGGAAGCTGCCCTTATTGCAGGCAGAGTTTTTCACAACCCCTAG

Protein sequence

MLLLLLLHVLCLAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKGNKCQVNEVMDVISFVMEAALIAGRVFHNP

Homology

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BLAST of Tan0021862 vs. ExPASy Swiss-Prot
Match: Q8S8N6 (Phospholipase A2-alpha OS=Arabidopsis thaliana OX=3702 GN=PLA2-ALPHA PE=1 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 3.2e-56
Identity = 98/138 (71.01%), Postives = 113/138 (81.88%), Query Frame = 0


Query: 12  LAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGER 71
           L   S    ALN+GVQ +  SI+L+K+CSRKCESEFCSVPP LRYGKYCGLLYSGCPGER
Sbjct: 11  LLFFSVSVSALNVGVQLIHPSISLTKECSRKCESEFCSVPPFLRYGKYCGLLYSGCPGER 70

Query: 72  PCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKGNKCQVNEVMDV 131
           PCDGLD+CCMKHDACV +KN+DYLS+ECS+ F+NCM NF + K   FKGNKC  +EV+DV
Sbjct: 71  PCDGLDSCCMKHDACVQSKNNDYLSQECSQKFINCMNNFSQKKQPTFKGNKCDADEVIDV 130

Query: 132 ISFVMEAALIAGRVFHNP 150
           IS VMEAALIAG+V   P
Sbjct: 131 ISIVMEAALIAGKVLKKP 148

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BLAST of Tan0021862 vs. ExPASy Swiss-Prot
Match: Q10E50 (Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III PE=1 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 5.3e-43
Identity = 82/141 (58.16%), Postives = 104/141 (73.76%), Query Frame = 0


Query: 9   VLCLAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP 68
           +L  A+V+ P  ALNIG+Q+     + +  CSR CES+ C+ PPLLRYGKYCG+LYSGCP
Sbjct: 25  LLLFAVVAPPAAALNIGLQSAGDGASKAGLCSRTCESDHCTTPPLLRYGKYCGILYSGCP 84

Query: 69  GERPCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKGNKCQVNEV 128
           GE+PCD LDACCM HD CV AKN DYLS  C+E  L C+    R  S+ F+GNKC ++EV
Sbjct: 85  GEQPCDELDACCMHHDNCVQAKN-DYLSTACNEELLECLARL-REGSSTFQGNKCMIDEV 144

Query: 129 MDVISFVMEAALIAGRVFHNP 150
           +DVIS V+EAA++AGR+ H P
Sbjct: 145 IDVISLVIEAAVVAGRLLHKP 163

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BLAST of Tan0021862 vs. ExPASy Swiss-Prot
Match: Q9XG81 (Probable phospholipase A2 homolog 2 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-II PE=1 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 1.5e-37
Identity = 74/139 (53.24%), Postives = 95/139 (68.35%), Query Frame = 0


Query: 9   VLCLAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP 68
           VL L ++   ++ LNIG   L ++    + CSR CES+FC++ PLLRYGKYCG+LYSGCP
Sbjct: 13  VLLLLLLVTASRGLNIG-DLLGSTPAKDQGCSRTCESQFCTIAPLLRYGKYCGILYSGCP 72

Query: 69  GERPCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKGNKCQVNEV 128
           GERPCD LDACCM HD CV   NDDYL+  C+EN L+C++   R   A F GNKC V + 
Sbjct: 73  GERPCDALDACCMVHDHCVDTHNDDYLNTMCNENLLSCID---RVSGATFPGNKCNVGQT 132

Query: 129 MDVISFVMEAALIAGRVFH 148
             VI  V+E A+ AG++ H
Sbjct: 133 ASVIRGVIETAVFAGKILH 147

[Close]

BLAST of Tan0021862 vs. ExPASy Swiss-Prot
Match: Q9XG80 (Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-I PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 3.3e-16
Identity = 44/107 (41.12%), Postives = 59/107 (55.14%), Query Frame = 0


Query: 39  CSRKCESEFCSVPPLLRYGKYCGLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSEE 98
           CSR C +  C     +RYGKYCG+ +SGC GE PCD LDACC  HD CV  K    +S +
Sbjct: 29  CSRSCAALNCDSVG-IRYGKYCGVGWSGCDGEEPCDDLDACCRDHDHCVDKKG--LMSVK 88

Query: 99  CSENFLNCMENFKRSKSAPFKGNKCQVNEVMDVISFVMEAALIAGRV 146
           C E F NCM   K++    F   KC     M  ++  M+ A++  ++
Sbjct: 89  CHEKFKNCMRKVKKAGKIGF-SRKCPYEMAMATMTSGMDMAIMLSQL 131

[Close]

BLAST of Tan0021862 vs. ExPASy Swiss-Prot
Match: Q8GV50 (Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.2e-15
Identity = 42/93 (45.16%), Postives = 56/93 (60.22%), Query Frame = 0


Query: 21  ALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGERPCDGLDACC 80
           AL + V  L A +   + CS+ C ++ C+V   +RYGKYCG+ Y GCPGE PCD LD CC
Sbjct: 11  ALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLG-IRYGKYCGIGYFGCPGEPPCDDLDDCC 70

Query: 81  MKHDACVVAKNDDYLSEECSENFLNCMENFKRS 114
           M HD CV  K   Y+  +C + F  C+   K+S
Sbjct: 71  MTHDNCVDLKGMTYV--DCHKQFQRCVNELKQS 100

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BLAST of Tan0021862 vs. NCBI nr
Match: XP_022932135.1 (phospholipase A2-alpha [Cucurbita moschata] >KAG6587708.1 Phospholipase A2-alpha, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021676.1 Phospholipase A2-alpha [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 269.2 bits (687), Expect = 2.1e-68
Identity = 124/141 (87.94%), Postives = 135/141 (95.74%), Query Frame = 0


Query: 9   VLCLAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP 68
           +LCLAIVSAPT ALN+GVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP
Sbjct: 12  LLCLAIVSAPTHALNVGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP 71

Query: 69  GERPCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKGNKCQVNEV 128
           GERPCDGLDACCM HDACV AKN+DYLSEECS++FLNCME FKRSKSAPFKGNKC+++EV
Sbjct: 72  GERPCDGLDACCMNHDACVAAKNNDYLSEECSKSFLNCMEKFKRSKSAPFKGNKCEISEV 131

Query: 129 MDVISFVMEAALIAGRVFHNP 150
           ++VISFVMEAAL+AGRV H P
Sbjct: 132 VEVISFVMEAALLAGRVLHKP 152

[Close]

BLAST of Tan0021862 vs. NCBI nr
Match: XP_008453533.1 (PREDICTED: phospholipase A2-alpha [Cucumis melo])

HSP 1 Score: 266.9 bits (681), Expect = 1.0e-67
Identity = 128/149 (85.91%), Postives = 140/149 (93.96%), Query Frame = 0


Query: 1   MLLLLLLHVLCLAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYC 60
           +LLLLLL +L  +I   P+ ALNIGVQALDASIT+SKDCSRKCESEFCSVPPLLRYGKYC
Sbjct: 8   LLLLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGKYC 67

Query: 61  GLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKG 120
           GLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLS+ECS++FLNCMENFKRS+S PFKG
Sbjct: 68  GLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSRPFKG 127

Query: 121 NKCQVNEVMDVISFVMEAALIAGRVFHNP 150
           NKCQV+EV+DVISFVMEAALIAG+VFH P
Sbjct: 128 NKCQVDEVIDVISFVMEAALIAGKVFHKP 156

[Close]

BLAST of Tan0021862 vs. NCBI nr
Match: XP_022973996.1 (phospholipase A2-alpha [Cucurbita maxima])

HSP 1 Score: 266.5 bits (680), Expect = 1.3e-67
Identity = 122/141 (86.52%), Postives = 134/141 (95.04%), Query Frame = 0


Query: 9   VLCLAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP 68
           +LCLAIVSAPT ALN+GVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP
Sbjct: 12  LLCLAIVSAPTHALNVGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP 71

Query: 69  GERPCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKGNKCQVNEV 128
           GERPCDGLDACCM HDACV AKN+DYLSEECS++FLNCME FKRSKSAPFKGNKC+++EV
Sbjct: 72  GERPCDGLDACCMNHDACVAAKNNDYLSEECSKSFLNCMEKFKRSKSAPFKGNKCEISEV 131

Query: 129 MDVISFVMEAALIAGRVFHNP 150
           ++VISFVM+ AL+AGRV H P
Sbjct: 132 VEVISFVMDVALLAGRVLHKP 152

[Close]

BLAST of Tan0021862 vs. NCBI nr
Match: XP_023529843.1 (phospholipase A2-alpha [Cucurbita pepo subsp. pepo])

HSP 1 Score: 266.5 bits (680), Expect = 1.3e-67
Identity = 123/143 (86.01%), Postives = 135/143 (94.41%), Query Frame = 0


Query: 7   LHVLCLAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSG 66
           L +LCLAIVSAPT ALN+GVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSG
Sbjct: 10  LLLLCLAIVSAPTHALNVGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSG 69

Query: 67  CPGERPCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKGNKCQVN 126
           CPGERPCDGLDACCM HDACV AKN+DYLSEECS++FLNCME FKRSK+ PFKGNKC+++
Sbjct: 70  CPGERPCDGLDACCMNHDACVAAKNNDYLSEECSKSFLNCMEKFKRSKAVPFKGNKCEIS 129

Query: 127 EVMDVISFVMEAALIAGRVFHNP 150
           EV++VISFVMEAAL+AGRV H P
Sbjct: 130 EVVEVISFVMEAALLAGRVLHKP 152

[Close]

BLAST of Tan0021862 vs. NCBI nr
Match: TYK28548.1 (phospholipase A2-alpha [Cucumis melo var. makuwa])

HSP 1 Score: 266.2 bits (679), Expect = 1.8e-67
Identity = 127/147 (86.39%), Postives = 138/147 (93.88%), Query Frame = 0


Query: 3   LLLLLHVLCLAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGL 62
           LLLLL +L  +I   P+ ALNIGVQALDASIT+SKDCSRKCESEFCSVPPLLRYGKYCGL
Sbjct: 5   LLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGKYCGL 64

Query: 63  LYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKGNK 122
           LYSGCPGERPCDGLDACCMKHDACVVAKNDDYLS+ECS++FLNCMENFKRS+S PFKGNK
Sbjct: 65  LYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSRPFKGNK 124

Query: 123 CQVNEVMDVISFVMEAALIAGRVFHNP 150
           CQV+EV+DVISFVMEAALIAG+VFH P
Sbjct: 125 CQVDEVIDVISFVMEAALIAGKVFHKP 151

[Close]

BLAST of Tan0021862 vs. ExPASy TrEMBL
Match: A0A6J1F1D2 (phospholipase A2-alpha OS=Cucurbita moschata OX=3662 GN=LOC111438456 PE=4 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 1.0e-68
Identity = 124/141 (87.94%), Postives = 135/141 (95.74%), Query Frame = 0


Query: 9   VLCLAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP 68
           +LCLAIVSAPT ALN+GVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP
Sbjct: 12  LLCLAIVSAPTHALNVGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP 71

Query: 69  GERPCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKGNKCQVNEV 128
           GERPCDGLDACCM HDACV AKN+DYLSEECS++FLNCME FKRSKSAPFKGNKC+++EV
Sbjct: 72  GERPCDGLDACCMNHDACVAAKNNDYLSEECSKSFLNCMEKFKRSKSAPFKGNKCEISEV 131

Query: 129 MDVISFVMEAALIAGRVFHNP 150
           ++VISFVMEAAL+AGRV H P
Sbjct: 132 VEVISFVMEAALLAGRVLHKP 152

[Close]

BLAST of Tan0021862 vs. ExPASy TrEMBL
Match: A0A1S3BWG7 (phospholipase A2-alpha OS=Cucumis melo OX=3656 GN=LOC103494216 PE=4 SV=1)

HSP 1 Score: 266.9 bits (681), Expect = 5.0e-68
Identity = 128/149 (85.91%), Postives = 140/149 (93.96%), Query Frame = 0


Query: 1   MLLLLLLHVLCLAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYC 60
           +LLLLLL +L  +I   P+ ALNIGVQALDASIT+SKDCSRKCESEFCSVPPLLRYGKYC
Sbjct: 8   LLLLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGKYC 67

Query: 61  GLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKG 120
           GLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLS+ECS++FLNCMENFKRS+S PFKG
Sbjct: 68  GLLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSRPFKG 127

Query: 121 NKCQVNEVMDVISFVMEAALIAGRVFHNP 150
           NKCQV+EV+DVISFVMEAALIAG+VFH P
Sbjct: 128 NKCQVDEVIDVISFVMEAALIAGKVFHKP 156

[Close]

BLAST of Tan0021862 vs. ExPASy TrEMBL
Match: A0A6J1IA72 (phospholipase A2-alpha OS=Cucurbita maxima OX=3661 GN=LOC111472609 PE=4 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 6.5e-68
Identity = 122/141 (86.52%), Postives = 134/141 (95.04%), Query Frame = 0


Query: 9   VLCLAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP 68
           +LCLAIVSAPT ALN+GVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP
Sbjct: 12  LLCLAIVSAPTHALNVGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCP 71

Query: 69  GERPCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKGNKCQVNEV 128
           GERPCDGLDACCM HDACV AKN+DYLSEECS++FLNCME FKRSKSAPFKGNKC+++EV
Sbjct: 72  GERPCDGLDACCMNHDACVAAKNNDYLSEECSKSFLNCMEKFKRSKSAPFKGNKCEISEV 131

Query: 129 MDVISFVMEAALIAGRVFHNP 150
           ++VISFVM+ AL+AGRV H P
Sbjct: 132 VEVISFVMDVALLAGRVLHKP 152

[Close]

BLAST of Tan0021862 vs. ExPASy TrEMBL
Match: A0A5D3DYM2 (Phospholipase A2-alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G001950 PE=4 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 8.5e-68
Identity = 127/147 (86.39%), Postives = 138/147 (93.88%), Query Frame = 0


Query: 3   LLLLLHVLCLAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGL 62
           LLLLL +L  +I   P+ ALNIGVQALDASIT+SKDCSRKCESEFCSVPPLLRYGKYCGL
Sbjct: 5   LLLLLLLLLPSIAFLPSHALNIGVQALDASITMSKDCSRKCESEFCSVPPLLRYGKYCGL 64

Query: 63  LYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKGNK 122
           LYSGCPGERPCDGLDACCMKHDACVVAKNDDYLS+ECS++FLNCMENFKRS+S PFKGNK
Sbjct: 65  LYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSRPFKGNK 124

Query: 123 CQVNEVMDVISFVMEAALIAGRVFHNP 150
           CQV+EV+DVISFVMEAALIAG+VFH P
Sbjct: 125 CQVDEVIDVISFVMEAALIAGKVFHKP 151

[Close]

BLAST of Tan0021862 vs. ExPASy TrEMBL
Match: A0A0A0LUV2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G433610 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 6.1e-66
Identity = 125/148 (84.46%), Postives = 137/148 (92.57%), Query Frame = 0


Query: 2   LLLLLLHVLCLAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCG 61
           LLLLLL     +IV  P+ ALNIGVQALDAS+T+SKDCSRKCESEFCSVPPLLRYGKYCG
Sbjct: 5   LLLLLLP----SIVFLPSHALNIGVQALDASVTMSKDCSRKCESEFCSVPPLLRYGKYCG 64

Query: 62  LLYSGCPGERPCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKGN 121
           LLYSGCPGE PCDGLDACCMKHDACVVAKNDDYLS+ECS++FLNCMENFKRS+S+PFKGN
Sbjct: 65  LLYSGCPGEHPCDGLDACCMKHDACVVAKNDDYLSQECSQSFLNCMENFKRSRSSPFKGN 124

Query: 122 KCQVNEVMDVISFVMEAALIAGRVFHNP 150
           KCQV+EV++VIS VMEAALIAGRVFH P
Sbjct: 125 KCQVDEVIEVISLVMEAALIAGRVFHKP 148

[Close]

BLAST of Tan0021862 vs. TAIR 10
Match: AT2G06925.1 (Phospholipase A2 family protein )

HSP 1 Score: 219.2 bits (557), Expect = 2.3e-57
Identity = 98/138 (71.01%), Postives = 113/138 (81.88%), Query Frame = 0


Query: 12  LAIVSAPTQALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGER 71
           L   S    ALN+GVQ +  SI+L+K+CSRKCESEFCSVPP LRYGKYCGLLYSGCPGER
Sbjct: 11  LLFFSVSVSALNVGVQLIHPSISLTKECSRKCESEFCSVPPFLRYGKYCGLLYSGCPGER 70

Query: 72  PCDGLDACCMKHDACVVAKNDDYLSEECSENFLNCMENFKRSKSAPFKGNKCQVNEVMDV 131
           PCDGLD+CCMKHDACV +KN+DYLS+ECS+ F+NCM NF + K   FKGNKC  +EV+DV
Sbjct: 71  PCDGLDSCCMKHDACVQSKNNDYLSQECSQKFINCMNNFSQKKQPTFKGNKCDADEVIDV 130

Query: 132 ISFVMEAALIAGRVFHNP 150
           IS VMEAALIAG+V   P
Sbjct: 131 ISIVMEAALIAGKVLKKP 148

[Close]

BLAST of Tan0021862 vs. TAIR 10
Match: AT4G29470.1 (Phospholipase A2 family protein )

HSP 1 Score: 84.3 bits (207), Expect = 8.8e-17
Identity = 42/93 (45.16%), Postives = 56/93 (60.22%), Query Frame = 0


Query: 21  ALNIGVQALDASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGERPCDGLDACC 80
           AL + V  L A +   + CS+ C ++ C+V   +RYGKYCG+ Y GCPGE PCD LD CC
Sbjct: 11  ALGLTVFLLLAVVHSQEKCSKTCIAQKCNVLG-IRYGKYCGIGYFGCPGEPPCDDLDDCC 70

Query: 81  MKHDACVVAKNDDYLSEECSENFLNCMENFKRS 114
           M HD CV  K   Y+  +C + F  C+   K+S
Sbjct: 71  MTHDNCVDLKGMTYV--DCHKQFQRCVNELKQS 100

[Close]

BLAST of Tan0021862 vs. TAIR 10
Match: AT4G29460.1 (Phospholipase A2 family protein )

HSP 1 Score: 79.7 bits (195), Expect = 2.2e-15
Identity = 37/83 (44.58%), Postives = 50/83 (60.24%), Query Frame = 0


Query: 31  ASITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGERPCDGLDACCMKHDACVVAK 90
           A ++  + CS  C ++ C+    +RYGKYCG+ Y GCPGE PCD LDACCM HD CV  K
Sbjct: 21  AVVSSQEKCSNTCIAQNCNSLG-IRYGKYCGIGYFGCPGEPPCDDLDACCMTHDNCVDLK 80

Query: 91  NDDYLSEECSENFLNCMENFKRS 114
              Y++  C + F  C+    +S
Sbjct: 81  GMTYVN--CHKQFKRCVNKLSKS 100

[Close]

BLAST of Tan0021862 vs. TAIR 10
Match: AT2G19690.2 (phospholipase A2-beta )

HSP 1 Score: 79.3 bits (194), Expect = 2.8e-15
Identity = 41/121 (33.88%), Postives = 66/121 (54.55%), Query Frame = 0


Query: 33  ITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGERPCDGLDACCMKHDACVVAKND 92
           +  S++C+R C ++ C     +RYGKYCG+ +SGCPGE PCD LDACC  HD CV     
Sbjct: 25  VARSEECTRTCIAQNCDTLS-IRYGKYCGIGHSGCPGEEPCDDLDACCKIHDHCVELNGM 84

Query: 93  DYLSEECSENFLNCMEN----FKRSKSAPFK-GNKCQVNEVMDVISFVMEAALIAGRVFH 149
             +S  C + F  C+       K+SK+       KC  + V+  ++  M+  ++  ++ +
Sbjct: 85  TNIS--CHKKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQGMDIGILFSQLAY 142

[Close]

BLAST of Tan0021862 vs. TAIR 10
Match: AT2G19690.1 (phospholipase A2-beta )

HSP 1 Score: 78.6 bits (192), Expect = 4.8e-15
Identity = 41/114 (35.96%), Postives = 62/114 (54.39%), Query Frame = 0


Query: 33  ITLSKDCSRKCESEFCSVPPLLRYGKYCGLLYSGCPGERPCDGLDACCMKHDACVVAKND 92
           +  S++C+R C ++ C     +RYGKYCG+ +SGCPGE PCD LDACC  HD CV     
Sbjct: 25  VARSEECTRTCIAQNCDTLS-IRYGKYCGIGHSGCPGEEPCDDLDACCKIHDHCVELNGM 84

Query: 93  DYLSEECSENFLNCMEN----FKRSKSAPFK-GNKCQVNEVMDVISFVMEAALI 142
             +S  C + F  C+       K+SK+       KC  + V+  ++  M+  ++
Sbjct: 85  TNIS--CHKKFQRCVNRLSKAIKQSKNKKVGFSTKCPYSVVIPTVNQGMDIGIL 135

The following BLAST results are available for this feature:

BLAST of Tan0021862 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S8N6	3.2e-56	71.01	Phospholipase A2-alpha OS=Arabidopsis thaliana OX=3702 GN=PLA2-ALPHA PE=1 SV=1	[more]
Q10E50	5.3e-43	58.16	Phospholipase A2 homolog 3 OS=Oryza sativa subsp. japonica OX=39947 GN=PLA2-III ...	[more]
Q9XG81	1.5e-37	53.24	Probable phospholipase A2 homolog 2 OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
Q9XG80	3.3e-16	41.12	Probable phospholipase A2 homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
Q8GV50	1.2e-15	45.16	Phospholipase A2-delta OS=Arabidopsis thaliana OX=3702 GN=PLA2-DELTA PE=2 SV=1	[more]

BLAST of Tan0021862 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022932135.1	2.1e-68	87.94	phospholipase A2-alpha [Cucurbita moschata] >KAG6587708.1 Phospholipase A2-alpha...	[more]
XP_008453533.1	1.0e-67	85.91	PREDICTED: phospholipase A2-alpha [Cucumis melo]	[more]
XP_022973996.1	1.3e-67	86.52	phospholipase A2-alpha [Cucurbita maxima]	[more]
XP_023529843.1	1.3e-67	86.01	phospholipase A2-alpha [Cucurbita pepo subsp. pepo]	[more]
TYK28548.1	1.8e-67	86.39	phospholipase A2-alpha [Cucumis melo var. makuwa]	[more]

BLAST of Tan0021862 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F1D2	1.0e-68	87.94	phospholipase A2-alpha OS=Cucurbita moschata OX=3662 GN=LOC111438456 PE=4 SV=1	[more]
A0A1S3BWG7	5.0e-68	85.91	phospholipase A2-alpha OS=Cucumis melo OX=3656 GN=LOC103494216 PE=4 SV=1	[more]
A0A6J1IA72	6.5e-68	86.52	phospholipase A2-alpha OS=Cucurbita maxima OX=3661 GN=LOC111472609 PE=4 SV=1	[more]
A0A5D3DYM2	8.5e-68	86.39	Phospholipase A2-alpha OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold6...	[more]
A0A0A0LUV2	6.1e-66	84.46	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G433610 PE=4 SV=1	[more]

BLAST of Tan0021862 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G06925.1	2.3e-57	71.01	Phospholipase A2 family protein	[more]
AT4G29470.1	8.8e-17	45.16	Phospholipase A2 family protein	[more]
AT4G29460.1	2.2e-15	44.58	Phospholipase A2 family protein	[more]
AT2G19690.2	2.8e-15	33.88	phospholipase A2-beta	[more]
AT2G19690.1	4.8e-15	35.96	phospholipase A2-beta	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036444	Phospholipase A2 domain superfamily	GENE3D	1.20.90.10	Phospholipase A2 domain	coord: 20..149 e-value: 2.5E-46 score: 158.5
IPR036444	Phospholipase A2 domain superfamily	SUPERFAMILY	48619	Phospholipase A2, PLA2	coord: 54..148
None	No IPR available	PANTHER	PTHR11716:SF87	PHOSPHOLIPASE A2 HOMOLOG 2-RELATED	coord: 9..146
None	No IPR available	CDD	cd04706	PLA2_plant	coord: 33..149 e-value: 4.40587E-56 score: 169.536
IPR001211	Phospholipase A2	PANTHER	PTHR11716	PHOSPHOLIPASE A2 FAMILY MEMBER	coord: 9..146
IPR033113	Phospholipase A2, histidine active site	PROSITE	PS00118	PA2_HIS	coord: 79..86

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0021862.1	Tan0021862.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050482	arachidonic acid secretion
biological_process	GO:0016042	lipid catabolic process
biological_process	GO:0006644	phospholipid metabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0005794	Golgi apparatus
molecular_function	GO:0047498	calcium-dependent phospholipase A2 activity
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0005543	phospholipid binding
molecular_function	GO:0004623	phospholipase A2 activity