Tan0021448 (gene) Snake gourd v1

BLAST of Tan0021448 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 178.7 bits (452), Expect = 9.3e-44
Identity = 101/261 (38.70%), Postives = 147/261 (56.32%), Query Frame = 0

Query: 25  PKNIFILAGQSNMAGRGGV-ENNQLGELVWDGYVPQECQPDPSILRLNPGRQWEIAREPI 84
           P  IFIL+GQSNMAGRGGV +++     VWD  +P EC P+ SILRL+   +WE A EP+
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 85  HEGIDIGKTVGIGPAMAFAHQLKAKAGPKAGVVGLVPCARGGTLIEQWIKNPSNPNATFY 144
           H  ID GK  G+GP MAFA+ +K +    + V+GLVPCA GGT I++W +      +  Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHLY 140

Query: 145 QNFIERIKASDKESGVVRALFWFQGESDAAMSDTASRYKGNLKNFLTDIRNDIKPRFLPI 204
           +  ++R + S K  G ++A+ W+QGESD      A  Y  N+   + ++R+D+    LPI
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 205 IVVKIALYDILMKHDTHDLPAVRAAEDAVQQELPDVVTIDSLKLPINFTTHEGFNQDHGH 264
           I V IA            +  VR A+  +  +L +VV +D+  LP+          D+ H
Sbjct: 201 IQVAIA-------SGGGYIDKVREAQ--LGLKLSNVVCVDAKGLPL--------KSDNLH 259

Query: 265 FNTTTEIALGKWLADTYLSHY 285
             T  ++ LG  LA  YLS++
Sbjct: 261 LTTEAQVQLGLSLAQAYLSNF 259

BLAST of Tan0021448 vs. NCBI nr
Match: XP_038886442.1 (probable carbohydrate esterase At4g34215 [Benincasa hispida])

HSP 1 Score: 510.0 bits (1312), Expect = 1.3e-140
Identity = 245/288 (85.07%), Postives = 261/288 (90.62%), Query Frame = 0

Query: 1   MTLLRLSILLCTMLFVSSLSGATSPKNIFILAGQSNMAGRGGVENNQLGELVWDGYVPQE 60
           M LL+LSILLCTMLF SSLS A SP NIFILAGQSNMAGRGGVENNQ+ EL WDG +P E
Sbjct: 1   MALLKLSILLCTMLFGSSLSRAASPNNIFILAGQSNMAGRGGVENNQVRELEWDGLIPPE 60

Query: 61  CQPDPSILRLNPGRQWEIAREPIHEGIDIGKTVGIGPAMAFAHQLKAKAGPKAGVVGLVP 120
           CQ DPSILRLNP  QWEIAREP+HEGIDI KTVGIGP M FAHQL  K GP+AG VGLVP
Sbjct: 61  CQSDPSILRLNPALQWEIAREPLHEGIDINKTVGIGPGMPFAHQLLTKVGPRAGTVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPNATFYQNFIERIKASDKESGVVRALFWFQGESDAAMSDTASR 180
           CARGGT+IEQWIKNPSNP+ATFY+NFIERIKASDKE GVVRALFWFQGESDAAMSDTA+R
Sbjct: 121 CARGGTIIEQWIKNPSNPDATFYKNFIERIKASDKEGGVVRALFWFQGESDAAMSDTANR 180

Query: 181 YKGNLKNFLTDIRNDIKPRFLPIIVVKIALYDILMKHDTHDLPAVRAAEDAVQQELPDVV 240
           YK NLKNF TDIRNDIKPRFLPII+VKIALYD +MKHDTHDLPAVRAA+DAV +ELPD+V
Sbjct: 181 YKDNLKNFFTDIRNDIKPRFLPIILVKIALYDFMMKHDTHDLPAVRAAQDAVSKELPDIV 240

Query: 241 TIDSLKLPINFTTHEGFNQDHGHFNTTTEIALGKWLADTYLSHYGHLL 289
           TID+LKLPIN  THEGFNQDHGHFNTTT+I LGKWLADTYLSHYGHLL
Sbjct: 241 TIDALKLPINVDTHEGFNQDHGHFNTTTQITLGKWLADTYLSHYGHLL 288

BLAST of Tan0021448 vs. NCBI nr
Match: XP_038886575.1 (probable carbohydrate esterase At4g34215 [Benincasa hispida])

HSP 1 Score: 501.5 bits (1290), Expect = 4.8e-138
Identity = 241/288 (83.68%), Postives = 261/288 (90.62%), Query Frame = 0

Query: 1   MTLLRLSILLCTMLFVSSLSGATSPKNIFILAGQSNMAGRGGVENNQLGELVWDGYVPQE 60
           M LL+L ILLCT+LF SSLS A SPKNIFILAGQSNMAGRGGVENNQ+G+L W+  +P E
Sbjct: 1   MALLKLLILLCTLLFGSSLSRAASPKNIFILAGQSNMAGRGGVENNQVGKLEWNRLIPPE 60

Query: 61  CQPDPSILRLNPGRQWEIAREPIHEGIDIGKTVGIGPAMAFAHQLKAKAGPKAGVVGLVP 120
           CQ D SILRLNP  QWE+AREP+HEGIDI KTVGIGP M FAHQL AK GPKAG+VGLVP
Sbjct: 61  CQSDTSILRLNPALQWEMAREPLHEGIDINKTVGIGPGMPFAHQLLAKVGPKAGIVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPNATFYQNFIERIKASDKESGVVRALFWFQGESDAAMSDTASR 180
           CA+GGT+IEQWIKNPSNP+ATFY++FIERIKASDKE GVVRALFWFQGESDAAM+DTASR
Sbjct: 121 CAKGGTIIEQWIKNPSNPDATFYKSFIERIKASDKEGGVVRALFWFQGESDAAMNDTASR 180

Query: 181 YKGNLKNFLTDIRNDIKPRFLPIIVVKIALYDILMKHDTHDLPAVRAAEDAVQQELPDVV 240
           YK NLKNF TDIRNDIKPRFLPII+VKIALYD +MKHDTHDLP VRAA+DAV +ELPDVV
Sbjct: 181 YKDNLKNFFTDIRNDIKPRFLPIILVKIALYDFMMKHDTHDLPVVRAAQDAVSKELPDVV 240

Query: 241 TIDSLKLPINFTTHEGFNQDHGHFNTTTEIALGKWLADTYLSHYGHLL 289
           TID+LKLPIN  THEGFNQDHGHFNTTTEI LGKWLADTYLSHYGHLL
Sbjct: 241 TIDALKLPINVDTHEGFNQDHGHFNTTTEITLGKWLADTYLSHYGHLL 288

BLAST of Tan0021448 vs. NCBI nr
Match: XP_023002177.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_023002870.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_023002871.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 489.6 bits (1259), Expect = 1.9e-134
Identity = 236/288 (81.94%), Postives = 256/288 (88.89%), Query Frame = 0

Query: 1   MTLLRLSILLCTMLFVSSLSGATSPKNIFILAGQSNMAGRGGVENNQLGELVWDGYVPQE 60
           M LL+LS LLC +LF  SLS ATSP NIFILAGQSNMAGRGGVENNQ G+L WDG VP E
Sbjct: 1   MILLKLSTLLCMILFHPSLSWATSPTNIFILAGQSNMAGRGGVENNQKGKLEWDGKVPLE 60

Query: 61  CQPDPSILRLNPGRQWEIAREPIHEGIDIGKTVGIGPAMAFAHQLKAKAGPKAGVVGLVP 120
           CQ DPSILRLNP RQWEIA+EP+H GIDIGKT GIGP + FAHQ KAKAG KAG+VGLVP
Sbjct: 61  CQSDPSILRLNPARQWEIAQEPLHLGIDIGKTPGIGPGIPFAHQFKAKAGQKAGIVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPNATFYQNFIERIKASDKESGVVRALFWFQGESDAAMSDTASR 180
           CARGGTLIEQWIKNPSNP+ATFYQNFIERIK S+KE GVVRALFW+QGESDAAMSDTA R
Sbjct: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMSDTAHR 180

Query: 181 YKGNLKNFLTDIRNDIKPRFLPIIVVKIALYDILMKHDTHDLPAVRAAEDAVQQELPDVV 240
           YK NLK F+TDIRNDIKPRFLP+I+VKI++YD  MKHDTHDLPAVRAAEDAVQ+ELPD++
Sbjct: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHDLPAVRAAEDAVQKELPDII 240

Query: 241 TIDSLKLPINFTTHEGFNQDHGHFNTTTEIALGKWLADTYLSHYGHLL 289
           TIDS +LPINFTT EGF  DHGHFNT TEIALGKWLADTYL+HY HLL
Sbjct: 241 TIDSWELPINFTTFEGFCLDHGHFNTATEIALGKWLADTYLAHYSHLL 288

BLAST of Tan0021448 vs. NCBI nr
Match: XP_022131651.1 (probable carbohydrate esterase At4g34215 [Momordica charantia])

HSP 1 Score: 484.6 bits (1246), Expect = 6.1e-133
Identity = 237/288 (82.29%), Postives = 256/288 (88.89%), Query Frame = 0

Query: 1   MTLLRLSILLCTMLFVSSLSGATSPKNIFILAGQSNMAGRGGVENNQLGELVWDGYVPQE 60
           M LLRLSI+LC MLF  SLSGATSPKNIFILAGQSNMAGRGGVE N+ G+L WDGYVP E
Sbjct: 1   MALLRLSIMLCMMLFGPSLSGATSPKNIFILAGQSNMAGRGGVEKNRTGDLEWDGYVPPE 60

Query: 61  CQPDPSILRLNPGRQWEIAREPIHEGIDIGKTVGIGPAMAFAHQLKAKAGPKAGVVGLVP 120
            QPDPSILRLNP RQWE+AREP+H GIDIGKTVG+GPA+AFAHQL+AK G K G VGLVP
Sbjct: 61  SQPDPSILRLNPERQWEVAREPVHRGIDIGKTVGVGPAIAFAHQLQAKGGSKVGSVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPNATFYQNFIERIKASDKESGVVRALFWFQGESDAAMSDTASR 180
           CARGGTLIEQW+KNPSNPNATFY+NFIERI+ASD+E GVVRALFW QGESDAA SDTA R
Sbjct: 121 CARGGTLIEQWVKNPSNPNATFYKNFIERIQASDREGGVVRALFWLQGESDAASSDTAER 180

Query: 181 YKGNLKNFLTDIRNDIKPRFLPIIVVKIALYDILMKHDTHDLPAVRAAEDAVQQELPDVV 240
           YK NLK F TDIRNDIKPR LPII+VKIA+YD  MKHDTHDLPAVRAAEDAVQ+ELP+VV
Sbjct: 181 YKNNLKKFFTDIRNDIKPRVLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNVV 240

Query: 241 TIDSLKLPINFTTHEGFNQDHGHFNTTTEIALGKWLADTYLSHYGHLL 289
           TID+LKL +N TT EGFN D GHFN  TEIALGKWLADTYLS+YGHLL
Sbjct: 241 TIDALKL-VNTTTAEGFNLDRGHFNIKTEIALGKWLADTYLSNYGHLL 287

BLAST of Tan0021448 vs. NCBI nr
Match: XP_022973316.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_022973317.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 483.8 bits (1244), Expect = 1.0e-132
Identity = 233/290 (80.34%), Postives = 255/290 (87.93%), Query Frame = 0

Query: 1   MTLLRLSILLCTMLFVSSLSGATSPKNIFILAGQSNMAGRGGVENNQLGELVWDGYVPQE 60
           M LL+LSIL+CT+L   SLSGATSP NIFILAGQSNMAGRGGVE    GELVWDG VP E
Sbjct: 1   MVLLKLSILMCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPTGELVWDGKVPSE 60

Query: 61  CQPDPSILRLNPGRQWEIAREPIHEGIDIG--KTVGIGPAMAFAHQLKAKAGPKAGVVGL 120
           CQ DPSILR NP RQWEIA EP+H GID+G  KT GIGP + FAHQLK KAG KAG+VGL
Sbjct: 61  CQSDPSILRFNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGL 120

Query: 121 VPCARGGTLIEQWIKNPSNPNATFYQNFIERIKASDKESGVVRALFWFQGESDAAMSDTA 180
           VPCARGGTLIEQWIKNPSNP+ATFYQNFIERIK S+KE GVVRALFW+QGESDAAM+DTA
Sbjct: 121 VPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTA 180

Query: 181 SRYKGNLKNFLTDIRNDIKPRFLPIIVVKIALYDILMKHDTHDLPAVRAAEDAVQQELPD 240
            RYK NLK F+TDIRNDIKPRFLP+I+VKIALYD  MKHDTH+LPAVRAAEDAVQ+ELPD
Sbjct: 181 QRYKDNLKKFITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPD 240

Query: 241 VVTIDSLKLPINFTTHEGFNQDHGHFNTTTEIALGKWLADTYLSHYGHLL 289
           ++TIDS +LP+N TT EGF+ DHGHFNT TEIALGKWLADTYL+HYGHLL
Sbjct: 241 IITIDSWELPMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 290

BLAST of Tan0021448 vs. ExPASy TrEMBL
Match: A0A6J1KIR8 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111496116 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 9.1e-135
Identity = 236/288 (81.94%), Postives = 256/288 (88.89%), Query Frame = 0

Query: 1   MTLLRLSILLCTMLFVSSLSGATSPKNIFILAGQSNMAGRGGVENNQLGELVWDGYVPQE 60
           M LL+LS LLC +LF  SLS ATSP NIFILAGQSNMAGRGGVENNQ G+L WDG VP E
Sbjct: 1   MILLKLSTLLCMILFHPSLSWATSPTNIFILAGQSNMAGRGGVENNQKGKLEWDGKVPLE 60

Query: 61  CQPDPSILRLNPGRQWEIAREPIHEGIDIGKTVGIGPAMAFAHQLKAKAGPKAGVVGLVP 120
           CQ DPSILRLNP RQWEIA+EP+H GIDIGKT GIGP + FAHQ KAKAG KAG+VGLVP
Sbjct: 61  CQSDPSILRLNPARQWEIAQEPLHLGIDIGKTPGIGPGIPFAHQFKAKAGQKAGIVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPNATFYQNFIERIKASDKESGVVRALFWFQGESDAAMSDTASR 180
           CARGGTLIEQWIKNPSNP+ATFYQNFIERIK S+KE GVVRALFW+QGESDAAMSDTA R
Sbjct: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMSDTAHR 180

Query: 181 YKGNLKNFLTDIRNDIKPRFLPIIVVKIALYDILMKHDTHDLPAVRAAEDAVQQELPDVV 240
           YK NLK F+TDIRNDIKPRFLP+I+VKI++YD  MKHDTHDLPAVRAAEDAVQ+ELPD++
Sbjct: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHDLPAVRAAEDAVQKELPDII 240

Query: 241 TIDSLKLPINFTTHEGFNQDHGHFNTTTEIALGKWLADTYLSHYGHLL 289
           TIDS +LPINFTT EGF  DHGHFNT TEIALGKWLADTYL+HY HLL
Sbjct: 241 TIDSWELPINFTTFEGFCLDHGHFNTATEIALGKWLADTYLAHYSHLL 288

BLAST of Tan0021448 vs. ExPASy TrEMBL
Match: A0A6J1BQ38 (probable carbohydrate esterase At4g34215 OS=Momordica charantia OX=3673 GN=LOC111004778 PE=4 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 2.9e-133
Identity = 237/288 (82.29%), Postives = 256/288 (88.89%), Query Frame = 0

Query: 1   MTLLRLSILLCTMLFVSSLSGATSPKNIFILAGQSNMAGRGGVENNQLGELVWDGYVPQE 60
           M LLRLSI+LC MLF  SLSGATSPKNIFILAGQSNMAGRGGVE N+ G+L WDGYVP E
Sbjct: 1   MALLRLSIMLCMMLFGPSLSGATSPKNIFILAGQSNMAGRGGVEKNRTGDLEWDGYVPPE 60

Query: 61  CQPDPSILRLNPGRQWEIAREPIHEGIDIGKTVGIGPAMAFAHQLKAKAGPKAGVVGLVP 120
            QPDPSILRLNP RQWE+AREP+H GIDIGKTVG+GPA+AFAHQL+AK G K G VGLVP
Sbjct: 61  SQPDPSILRLNPERQWEVAREPVHRGIDIGKTVGVGPAIAFAHQLQAKGGSKVGSVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPNATFYQNFIERIKASDKESGVVRALFWFQGESDAAMSDTASR 180
           CARGGTLIEQW+KNPSNPNATFY+NFIERI+ASD+E GVVRALFW QGESDAA SDTA R
Sbjct: 121 CARGGTLIEQWVKNPSNPNATFYKNFIERIQASDREGGVVRALFWLQGESDAASSDTAER 180

Query: 181 YKGNLKNFLTDIRNDIKPRFLPIIVVKIALYDILMKHDTHDLPAVRAAEDAVQQELPDVV 240
           YK NLK F TDIRNDIKPR LPII+VKIA+YD  MKHDTHDLPAVRAAEDAVQ+ELP+VV
Sbjct: 181 YKNNLKKFFTDIRNDIKPRVLPIILVKIAVYDTFMKHDTHDLPAVRAAEDAVQRELPNVV 240

Query: 241 TIDSLKLPINFTTHEGFNQDHGHFNTTTEIALGKWLADTYLSHYGHLL 289
           TID+LKL +N TT EGFN D GHFN  TEIALGKWLADTYLS+YGHLL
Sbjct: 241 TIDALKL-VNTTTAEGFNLDRGHFNIKTEIALGKWLADTYLSNYGHLL 287

BLAST of Tan0021448 vs. ExPASy TrEMBL
Match: A0A6J1I774 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111471873 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 5.0e-133
Identity = 233/290 (80.34%), Postives = 255/290 (87.93%), Query Frame = 0

Query: 1   MTLLRLSILLCTMLFVSSLSGATSPKNIFILAGQSNMAGRGGVENNQLGELVWDGYVPQE 60
           M LL+LSIL+CT+L   SLSGATSP NIFILAGQSNMAGRGGVE    GELVWDG VP E
Sbjct: 1   MVLLKLSILMCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPTGELVWDGKVPSE 60

Query: 61  CQPDPSILRLNPGRQWEIAREPIHEGIDIG--KTVGIGPAMAFAHQLKAKAGPKAGVVGL 120
           CQ DPSILR NP RQWEIA EP+H GID+G  KT GIGP + FAHQLK KAG KAG+VGL
Sbjct: 61  CQSDPSILRFNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGL 120

Query: 121 VPCARGGTLIEQWIKNPSNPNATFYQNFIERIKASDKESGVVRALFWFQGESDAAMSDTA 180
           VPCARGGTLIEQWIKNPSNP+ATFYQNFIERIK S+KE GVVRALFW+QGESDAAM+DTA
Sbjct: 121 VPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTA 180

Query: 181 SRYKGNLKNFLTDIRNDIKPRFLPIIVVKIALYDILMKHDTHDLPAVRAAEDAVQQELPD 240
            RYK NLK F+TDIRNDIKPRFLP+I+VKIALYD  MKHDTH+LPAVRAAEDAVQ+ELPD
Sbjct: 181 QRYKDNLKKFITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPD 240

Query: 241 VVTIDSLKLPINFTTHEGFNQDHGHFNTTTEIALGKWLADTYLSHYGHLL 289
           ++TIDS +LP+N TT EGF+ DHGHFNT TEIALGKWLADTYL+HYGHLL
Sbjct: 241 IITIDSWELPMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 290

BLAST of Tan0021448 vs. ExPASy TrEMBL
Match: A0A0A0LNC5 (SASA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G356040 PE=4 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 5.5e-132
Identity = 233/288 (80.90%), Postives = 252/288 (87.50%), Query Frame = 0

Query: 1   MTLLRLSILLCTMLFVSSLSGATSPKNIFILAGQSNMAGRGGVENNQLGELVWDGYVPQE 60
           M LLRLSI+LC ML+  SLSGA SPKNIFILAGQSNMAGRGGVENN  G L WDG VP E
Sbjct: 1   MVLLRLSIILCVMLYGPSLSGAASPKNIFILAGQSNMAGRGGVENNAQGNLQWDGLVPPE 60

Query: 61  CQPDPSILRLNPGRQWEIAREPIHEGIDIGKTVGIGPAMAFAHQLKAKAGPKAGVVGLVP 120
           CQP PSILRLNPG QWEIAREP+H GIDI +T GIGP +AFAH+L  KAGP AG VGLVP
Sbjct: 61  CQPQPSILRLNPGLQWEIAREPLHLGIDIKRTPGIGPGIAFAHELLVKAGPNAGAVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPNATFYQNFIERIKASDKESGVVRALFWFQGESDAAMSDTASR 180
           CARGGTLIEQWIKNPSNP+ATFYQNFIERIKASDK+ GVVRALFWFQGESDAAM+DTA R
Sbjct: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKASDKDGGVVRALFWFQGESDAAMNDTAIR 180

Query: 181 YKGNLKNFLTDIRNDIKPRFLPIIVVKIALYDILMKHDTHDLPAVRAAEDAVQQELPDVV 240
           YK NLK F TDIR+DIKPRFLPIIVVKIALYD   +HDTH+LPAVR A++AV +ELPDVV
Sbjct: 181 YKDNLKKFFTDIRDDIKPRFLPIIVVKIALYDFFRQHDTHNLPAVREAKEAVSKELPDVV 240

Query: 241 TIDSLKLPINFTTHEGFNQDHGHFNTTTEIALGKWLADTYLSHYGHLL 289
            IDSLKLPIN+TT+EG N DHGHFNTTTEI LGKWLA+TYLSH+G LL
Sbjct: 241 AIDSLKLPINYTTNEGINLDHGHFNTTTEITLGKWLAETYLSHFGQLL 288

BLAST of Tan0021448 vs. ExPASy TrEMBL
Match: A0A6J1GJF6 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454406 PE=4 SV=1)

HSP 1 Score: 479.9 bits (1234), Expect = 7.2e-132
Identity = 231/288 (80.21%), Postives = 254/288 (88.19%), Query Frame = 0

Query: 1   MTLLRLSILLCTMLFVSSLSGATSPKNIFILAGQSNMAGRGGVENNQLGELVWDGYVPQE 60
           M LL+LSILLC +LF  SLSGATSP NIFILAGQSNMAGRGGVE  Q G+LVWDG VP E
Sbjct: 1   MVLLKLSILLCMILFNPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLE 60

Query: 61  CQPDPSILRLNPGRQWEIAREPIHEGIDIGKTVGIGPAMAFAHQLKAKAGPKAGVVGLVP 120
           CQ DPSILRLNP RQWEIA EP+H GIDI  T GIGP + FAHQ K KAG KAG+VGLVP
Sbjct: 61  CQFDPSILRLNPERQWEIAHEPLHLGIDISHTPGIGPGIPFAHQFKEKAGQKAGIVGLVP 120

Query: 121 CARGGTLIEQWIKNPSNPNATFYQNFIERIKASDKESGVVRALFWFQGESDAAMSDTASR 180
           CARGGTLIEQWIKNPSNP+ATFYQNFIERIK S+KE GVVRALFW+QGESDAAM+DTA R
Sbjct: 121 CARGGTLIEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQR 180

Query: 181 YKGNLKNFLTDIRNDIKPRFLPIIVVKIALYDILMKHDTHDLPAVRAAEDAVQQELPDVV 240
           YK NLK F+TDIRNDIKPRFLP+I+VKI+LYD  MKHDTH+LPAVRAAEDAVQ+ELPD++
Sbjct: 181 YKDNLKKFITDIRNDIKPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDII 240

Query: 241 TIDSLKLPINFTTHEGFNQDHGHFNTTTEIALGKWLADTYLSHYGHLL 289
           TIDS +LP+NFTT EGF+ DHGHFNT TEI LGKWLA+TYL+HYGHLL
Sbjct: 241 TIDSRELPVNFTTFEGFSWDHGHFNTETEIVLGKWLANTYLAHYGHLL 288

BLAST of Tan0021448 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 178.7 bits (452), Expect = 6.6e-45
Identity = 101/261 (38.70%), Postives = 147/261 (56.32%), Query Frame = 0

Query: 25  PKNIFILAGQSNMAGRGGV-ENNQLGELVWDGYVPQECQPDPSILRLNPGRQWEIAREPI 84
           P  IFIL+GQSNMAGRGGV +++     VWD  +P EC P+ SILRL+   +WE A EP+
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 85  HEGIDIGKTVGIGPAMAFAHQLKAKAGPKAGVVGLVPCARGGTLIEQWIKNPSNPNATFY 144
           H  ID GK  G+GP MAFA+ +K +    + V+GLVPCA GGT I++W +      +  Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHLY 140

Query: 145 QNFIERIKASDKESGVVRALFWFQGESDAAMSDTASRYKGNLKNFLTDIRNDIKPRFLPI 204
           +  ++R + S K  G ++A+ W+QGESD      A  Y  N+   + ++R+D+    LPI
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 205 IVVKIALYDILMKHDTHDLPAVRAAEDAVQQELPDVVTIDSLKLPINFTTHEGFNQDHGH 264
           I V IA            +  VR A+  +  +L +VV +D+  LP+          D+ H
Sbjct: 201 IQVAIA-------SGGGYIDKVREAQ--LGLKLSNVVCVDAKGLPL--------KSDNLH 259

Query: 265 FNTTTEIALGKWLADTYLSHY 285
             T  ++ LG  LA  YLS++
Sbjct: 261 LTTEAQVQLGLSLAQAYLSNF 259

BLAST of Tan0021448 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 178.7 bits (452), Expect = 6.6e-45
Identity = 101/261 (38.70%), Postives = 147/261 (56.32%), Query Frame = 0

Query: 25  PKNIFILAGQSNMAGRGGV-ENNQLGELVWDGYVPQECQPDPSILRLNPGRQWEIAREPI 84
           P  IFIL+GQSNMAGRGGV +++     VWD  +P EC P+ SILRL+   +WE A EP+
Sbjct: 21  PNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLRWEEAHEPL 80

Query: 85  HEGIDIGKTVGIGPAMAFAHQLKAKAGPKAGVVGLVPCARGGTLIEQWIKNPSNPNATFY 144
           H  ID GK  G+GP MAFA+ +K +    + V+GLVPCA GGT I++W +      +  Y
Sbjct: 81  HVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER-----GSHLY 140

Query: 145 QNFIERIKASDKESGVVRALFWFQGESDAAMSDTASRYKGNLKNFLTDIRNDIKPRFLPI 204
           +  ++R + S K  G ++A+ W+QGESD      A  Y  N+   + ++R+D+    LPI
Sbjct: 141 ERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPSLPI 200

Query: 205 IVVKIALYDILMKHDTHDLPAVRAAEDAVQQELPDVVTIDSLKLPINFTTHEGFNQDHGH 264
           I V IA            +  VR A+  +  +L +VV +D+  LP+          D+ H
Sbjct: 201 IQVAIA-------SGGGYIDKVREAQ--LGLKLSNVVCVDAKGLPL--------KSDNLH 259

Query: 265 FNTTTEIALGKWLADTYLSHY 285
             T  ++ LG  LA  YLS++
Sbjct: 261 LTTEAQVQLGLSLAQAYLSNF 259

BLAST of Tan0021448 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 173.7 bits (439), Expect = 2.1e-43
Identity = 105/259 (40.54%), Postives = 149/259 (57.53%), Query Frame = 0

Query: 27  NIFILAGQSNMAGRGGVENN-QLGELVWDGYVPQECQPDPSILRLNPGRQWEIAREPIHE 86
           +IFILAGQSNMAGRGGV N+      VWDG +P EC+ +PSILRL    +W+ A+EP+H 
Sbjct: 30  SIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSKLEWKEAKEPLHV 89

Query: 87  GIDIGKTVGIGPAMAFAHQLKAKAGPKAGVVGLVPCARGGTLIEQWIKNPSNPNATFYQN 146
            IDI KT G+GP M FA+++  + G     VGLVPC+ GGT + QW K         Y+ 
Sbjct: 90  DIDINKTNGVGPGMPFANRVVNRFGQ----VGLVPCSIGGTKLSQWQK-----GEFLYEE 149

Query: 147 FIERIKA--SDKESGVVRALFWFQGESDAAMSDTASRYKGNLKNFLTDIRNDIKPRFLPI 206
            ++R KA  +    G  RA+ W+QGESD      AS YK  L  F +D+RND++   LPI
Sbjct: 150 TVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFFSDLRNDLQHPNLPI 209

Query: 207 IVVKIALYDILMKHDTHDLPAVRAAEDAVQQELPDVVTIDSLKLPINFTTHEGFNQDHGH 266
           I V +A            L AVR A+  ++ +L +V  +D+  LP+          D  H
Sbjct: 210 IQVALA------TGAGPYLDAVRKAQ--LKTDLENVYCVDARGLPL--------EPDGLH 263

Query: 267 FNTTTEIALGKWLADTYLS 283
             T++++ LG  +A+++L+
Sbjct: 270 LTTSSQVQLGHMIAESFLA 263