Tan0021329 (gene) Snake gourd v1

Overview


Name	Tan0021329
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Mediator of RNA polymerase II transcription subunit 20
Location	LG08: 441980 .. 443811 (+)
RNA-Seq Expression	Tan0021329
Synteny	Tan0021329

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGCTGAAATGGTAAAACCCCCGACCAAACACTTCTCCTCCCTTTCGCGTGGATTTGATTCTTGATCTAGTTTCTTTGTTTTCTTTTCCTTAGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACTGTTAACAGTCAGATCCTCAACGAAGTTTCCCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTACCCTCACTTTCTACAAACCCATCTTACGAGGTAATCGACCTCCTTTCCTAGAGGCGTTTGTCCGGATTTGATTACCTAAATGAATTTTAAATCTGTCAACAGACCAGAACTTACAGGCGGCCGACTTCCCGCGCGACTTTCTCGGAATTTCGCTGCCGGAGCAACCAAACAAGTATTATCTTATAATTCGTGGCCAACGGATCGTCTTGGAAGCTGATTTTTCGATTCAGACTATAATGGAGAAGCTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGTTTACTTGACCACCCAACTTTTCTGGTTCTGATGTACTCTGCATTTTCCCATATCCTGAAATTCAAATTTGAAGTTGGTTTTTGTAGCTCTCTGGTCTCTACTCTTTTGCCCTTTTGTTTCTAGTCGTGTGAGATGGATAAATGTTTGGTTACAGACAGTTCACCTGGTTGTTTTTCAGGGGTTCCAATACCAATTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCTAATCATTCTGAGAATTTGAGAGGAATAGTGATGGAGGTAAGACTCATTTCTTCATCATTTCATTTTAATTCTGTTCTTGTGTTATTGCATTCTCTTCTGGTTGGTTTTTTGTGTCTTTTTCTTTGTCTGAGCCAGCAGTTTCTTTTTCATGAGCTGTTACAAAACAATGTTTGGTCTCTGTTGTGCTTATGTTGAGAAGTAGTGAGATCTGATTAGGATTTCTTTGTAAAAGCCTGTGTAGGAATAGTTATGGAAGTCGTTGTGATATTGCACATGAATCGAAGTGTTTTGAGTAAATTCCTCTCCAAGAGTTGATTTTATTCTGTTTGGGATGTGCCTTTCAGTTCACTTGGATGCTTGATGACCGTGTATAGTCTTTGCTTGCGCTCGATTCCTTGTGATGCTCATCCTGAAACAAATAAAGCAAAGAAAGACTCCGATTTTGAACATCTGTTCTTATTTGTCTTTTCTTTTTTCCTCTTCATATTATTCTTCAAAAACACATTACCATGTTCTTTATTGTATTGTCAAGTCCAAGTAGTATATACTATCAAGTCTGTCAAACGTGCATTCTTAGATGGATTGCTTGCAGGCTGAAGTTGGAAACCTGAAACTTCTCTAGCAAATCTATTATTCACTTCTGTAATTTGTTTTTAATCTAATCTGATGAATGCCACATTGGAAGTATTGTTAAATTTGGGTTCAAGTGGGATTTCATTAGAAACTAGAATCTGTCTATCTAATGTGCAAATGTTATATTTTTGCACTGTGAATATGAATATTGCTGATTGTTGCTGATCCAATGAGACTGTATACCATTCCTGTTCTAGAATTGAAATGTATTCAATCCTGTATATTGCAGATTGAGTATCTCCCAATTTCATCAATGGAGAAATCAAGACAGGTGATGGAGGAGTTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCACTTCCAGGTCGTTTCATGCACATAGAACCAAACTTTGCTGAATATGGACTGTCCGATTACTACACTTCTCAACACACTGCGGTTCAGTATGCCAATGTCATGGCTCAGCTAATCGCTACTGTGCAAGCAGTTCAAGCAGTGAGAAATTAG

mRNA sequence

ATGCCGCTGAAATGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACTGTTAACAGTCAGATCCTCAACGAAGTTTCCCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTACCCTCACTTTCTACAAACCCATCTTACGAGACCAGAACTTACAGGCGGCCGACTTCCCGCGCGACTTTCTCGGAATTTCGCTGCCGGAGCAACCAAACAAGTATTATCTTATAATTCGTGGCCAACGGATCGTCTTGGAAGCTGATTTTTCGATTCAGACTATAATGGAGAAGCTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGGGTTCCAATACCAATTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCTAATCATTCTGAGAATTTGAGAGGAATAGTGATGGAGATTGAGTATCTCCCAATTTCATCAATGGAGAAATCAAGACAGGTGATGGAGGAGTTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCACTTCCAGGTCGTTTCATGCACATAGAACCAAACTTTGCTGAATATGGACTGTCCGATTACTACACTTCTCAACACACTGCGGTTCAGTATGCCAATGTCATGGCTCAGCTAATCGCTACTGTGCAAGCAGTTCAAGCAGTGAGAAATTAG

Coding sequence (CDS)

ATGCCGCTGAAATGGGTTTTGCATTGGCAACCGAATGCCGGCAGCACTGTTAACAGTCAGATCCTCAACGAAGTTTCCCAATGTGTTGAGAGTATCCATGGCGTTAAAGGGGGCAAGTGGAAAGCTACCCTCACTTTCTACAAACCCATCTTACGAGACCAGAACTTACAGGCGGCCGACTTCCCGCGCGACTTTCTCGGAATTTCGCTGCCGGAGCAACCAAACAAGTATTATCTTATAATTCGTGGCCAACGGATCGTCTTGGAAGCTGATTTTTCGATTCAGACTATAATGGAGAAGCTGCAGTCTTACAAATTGCGCGTTGCTCTCAATTTCGAGGGGTTCCAATACCAATTAGGGGATTTCCAACTGAGAGTGGGAAAAGTTGTTCCTAATCATTCTGAGAATTTGAGAGGAATAGTGATGGAGATTGAGTATCTCCCAATTTCATCAATGGAGAAATCAAGACAGGTGATGGAGGAGTTCTTCGAGGTATGGCAAGAAGCCATGTCAAAAAGTTCACTTCCAGGTCGTTTCATGCACATAGAACCAAACTTTGCTGAATATGGACTGTCCGATTACTACACTTCTCAACACACTGCGGTTCAGTATGCCAATGTCATGGCTCAGCTAATCGCTACTGTGCAAGCAGTTCAAGCAGTGAGAAATTAG

Protein sequence

MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAADFPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQAVRN

Homology

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BLAST of Tan0021329 vs. ExPASy Swiss-Prot
Match: Q6NPF4 (Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana OX=3702 GN=MED20A PE=1 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 7.1e-92
Identity = 164/219 (74.89%), Postives = 193/219 (88.13%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAAD 60
           MP+KWVLHWQPN GSTV+SQILNE +QCVESI+GVK G+WKATL +YKP+L+DQ  Q  +
Sbjct: 1   MPVKWVLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKDQANQ-LE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISL +QPNKYY IIR QRIVLEAD SIQ IMEKLQSYK +VAL F+GFQYQLG
Sbjct: 61  FPRDFLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQLG 120

Query: 121 DFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DF+LRVGKVVP HSEN+RGIVME+EYLPISSMEK+++VMEEF E+W EA++K SLPG+F+
Sbjct: 121 DFRLRVGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFV 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ 220
           +I+ NF E+GL D YT QHTAV+YA VMA +IATVQAV+
Sbjct: 181 NIDLNFGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVR 218

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BLAST of Tan0021329 vs. ExPASy Swiss-Prot
Match: Q9M0S0 (Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana OX=3702 GN=MED20B PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 3.9e-82
Identity = 144/217 (66.36%), Postives = 182/217 (83.87%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAAD 60
           MP+KW+LHWQPN GST +SQILNEV+Q +ES++GVK G WKATL +YKP+L+DQ  Q A+
Sbjct: 1   MPVKWLLHWQPNQGSTFSSQILNEVTQSIESLNGVKEGTWKATLNYYKPMLQDQANQ-AE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLG 120
           FPR+F+GISLPE+P+KYY +IR QRIV+EAD SIQ IME LQSYK +++  FEG +YQLG
Sbjct: 61  FPREFVGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFEGLEYQLG 120

Query: 121 DFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DF+LRVGKVVP H+E +RG+VME+EYLPISSM  ++++MEEF E+WQEAMSK SLPG+F+
Sbjct: 121 DFRLRVGKVVPTHAETIRGVVMEVEYLPISSMGMAKKLMEEFLEIWQEAMSKRSLPGKFV 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQA 218
           + E NF ++GL D YT QHTAV YA  MA L+A +QA
Sbjct: 181 NKELNFEKFGLGDNYTPQHTAVGYAFFMANLMAAIQA 216

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BLAST of Tan0021329 vs. ExPASy Swiss-Prot
Match: Q54FL3 (Putative mediator of RNA polymerase II transcription subunit 20 OS=Dictyostelium discoideum OX=44689 GN=med20 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.6e-09
Identity = 56/197 (28.43%), Postives = 92/197 (46.70%), Query Frame = 0


Query: 22  LNEVSQCVESIHGVKGGKWKATLTFYKPILRD-QNLQAADFPRDFLGISLPEQPNKYYLI 81
           L E+++ +E + G+K        + Y   + D  NL     PR+F  +   E+P K + +
Sbjct: 13  LPEMTKRIEFLGGIKVSTGTINCSLYLERVPDGSNL----IPREFHLLLYDEKPKKCFTV 72

Query: 82  IRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLGDFQLRVGKVVPNHSENLRGI 141
            R    ++E D  + +I+EK  SYK R        +Y+LGDF +RVG +V      +RGI
Sbjct: 73  CRD--TIVETDREMVSILEKTNSYKKRQTTEALISKYELGDFIIRVGPIV--FRTEIRGI 132

Query: 142 VMEIEY----LPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDY-- 201
           ++E+EY     P++S     +++ EF          +   G F   EP    +  S Y  
Sbjct: 133 LVEVEYTACSTPVTSPFNCTKLLSEFI---------NGNLGPFP--EPIQTSFDFSAYPT 190

Query: 202 ----YTSQHTAVQYANV 208
               Y+  HT+ QY N+
Sbjct: 193 LPKSYSDLHTSYQYVNL 190

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BLAST of Tan0021329 vs. NCBI nr
Match: XP_038889134.1 (mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Benincasa hispida])

HSP 1 Score: 436.4 bits (1121), Expect = 1.5e-118
Identity = 215/223 (96.41%), Postives = 220/223 (98.65%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAAD 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNL AA+
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADF+IQ IMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPN+SENLRGIVMEIEYLPISSMEKSR+VMEEFFEVWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRKVMEEFFEVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQAVRN 224
           HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ+ RN
Sbjct: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

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BLAST of Tan0021329 vs. NCBI nr
Match: XP_022946745.1 (mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata] >XP_022999230.1 mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita maxima] >XP_023546929.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023546930.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X2 [Cucurbita pepo subsp. pepo] >KAG6599532.1 Mediator of RNA polymerase II transcription subunit 20a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 431.0 bits (1107), Expect = 6.1e-117
Identity = 211/223 (94.62%), Postives = 218/223 (97.76%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAAD 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N+ A +
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADF+IQ IMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPN+SENLRGIVMEIEYLPISSMEKS+QVMEEFFEVWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQAVRN 224
           HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ+ RN
Sbjct: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of Tan0021329 vs. NCBI nr
Match: XP_004141069.1 (mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60509.1 hypothetical protein Csa_019466 [Cucumis sativus])

HSP 1 Score: 429.9 bits (1104), Expect = 1.4e-116
Identity = 212/223 (95.07%), Postives = 217/223 (97.31%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAAD 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN  AA+
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADF+IQ IMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPN+SENLRGIVMEIEYLPISSMEKSRQVMEEFF+VWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQAVRN 224
           HIE NFAEYGLSDYYTSQHTAVQY NVMAQLIATVQAVQ+ RN
Sbjct: 181 HIESNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of Tan0021329 vs. NCBI nr
Match: XP_008459711.1 (PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo])

HSP 1 Score: 424.9 bits (1091), Expect = 4.4e-115
Identity = 208/223 (93.27%), Postives = 216/223 (96.86%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAAD 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN  AA+
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADF+IQ IMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPN+SENLRGIVMEIEYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQAVRN 224
           HIE NFAEYGLSD+YT QHTAVQY NVMAQLIATVQAVQ+ RN
Sbjct: 181 HIESNFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of Tan0021329 vs. NCBI nr
Match: KAA0061469.1 (mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo var. makuwa] >TYK10805.1 mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 424.9 bits (1091), Expect = 4.4e-115
Identity = 208/223 (93.27%), Postives = 216/223 (96.86%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAAD 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN  AA+
Sbjct: 49  MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 108

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADF+IQ IMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 109 FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 168

Query: 121 DFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPN+SENLRGIVMEIEYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRFM
Sbjct: 169 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFM 228

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQAVRN 224
           HIE NFAEYGLSD+YT QHTAVQY NVMAQLIATVQAVQ+ RN
Sbjct: 229 HIESNFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 271

[Close]

BLAST of Tan0021329 vs. ExPASy TrEMBL
Match: A0A6J1KEU1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=3661 GN=LOC111493666 PE=3 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 3.0e-117
Identity = 211/223 (94.62%), Postives = 218/223 (97.76%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAAD 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N+ A +
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADF+IQ IMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPN+SENLRGIVMEIEYLPISSMEKS+QVMEEFFEVWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQAVRN 224
           HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ+ RN
Sbjct: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of Tan0021329 vs. ExPASy TrEMBL
Match: A0A6J1G4U3 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=3662 GN=LOC111450720 PE=3 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 3.0e-117
Identity = 211/223 (94.62%), Postives = 218/223 (97.76%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAAD 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRD N+ A +
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDTNIAAGE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGIS+PEQPNKYYLIIRGQRIVLEADF+IQ IMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISVPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPN+SENLRGIVMEIEYLPISSMEKS+QVMEEFFEVWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSKQVMEEFFEVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQAVRN 224
           HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ+ RN
Sbjct: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQSARN 223

[Close]

BLAST of Tan0021329 vs. ExPASy TrEMBL
Match: A0A0A0LHQ1 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=3659 GN=MED20 PE=3 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 6.6e-117
Identity = 212/223 (95.07%), Postives = 217/223 (97.31%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAAD 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN  AA+
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADF+IQ IMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPN+SENLRGIVMEIEYLPISSMEKSRQVMEEFF+VWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQVMEEFFDVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQAVRN 224
           HIE NFAEYGLSDYYTSQHTAVQY NVMAQLIATVQAVQ+ RN
Sbjct: 181 HIESNFAEYGLSDYYTSQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of Tan0021329 vs. ExPASy TrEMBL
Match: A0A5D3CH02 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. makuwa OX=1194695 GN=MED20 PE=3 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 2.1e-115
Identity = 208/223 (93.27%), Postives = 216/223 (96.86%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAAD 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN  AA+
Sbjct: 49  MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 108

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADF+IQ IMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 109 FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 168

Query: 121 DFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPN+SENLRGIVMEIEYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRFM
Sbjct: 169 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFM 228

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQAVRN 224
           HIE NFAEYGLSD+YT QHTAVQY NVMAQLIATVQAVQ+ RN
Sbjct: 229 HIESNFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 271

[Close]

BLAST of Tan0021329 vs. ExPASy TrEMBL
Match: A0A1S3CAW6 (Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 GN=LOC103498750 PE=3 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 2.1e-115
Identity = 208/223 (93.27%), Postives = 216/223 (96.86%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAAD 60
           MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQN  AA+
Sbjct: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNSPAAE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISLPEQPNKYYLIIRGQRIVLEADF+IQ IMEKLQSYKLRVALNFEGFQYQLG
Sbjct: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFTIQAIMEKLQSYKLRVALNFEGFQYQLG 120

Query: 121 DFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DFQLRVGKVVPN+SENLRGIVMEIEYLPISSMEKSRQ+M+EFF+VWQEAMSKSSLPGRFM
Sbjct: 121 DFQLRVGKVVPNNSENLRGIVMEIEYLPISSMEKSRQIMDEFFDVWQEAMSKSSLPGRFM 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQAVRN 224
           HIE NFAEYGLSD+YT QHTAVQY NVMAQLIATVQAVQ+ RN
Sbjct: 181 HIESNFAEYGLSDFYTPQHTAVQYGNVMAQLIATVQAVQSARN 223

[Close]

BLAST of Tan0021329 vs. TAIR 10
Match: AT2G28230.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 338.2 bits (866), Expect = 5.1e-93
Identity = 164/219 (74.89%), Postives = 193/219 (88.13%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAAD 60
           MP+KWVLHWQPN GSTV+SQILNE +QCVESI+GVK G+WKATL +YKP+L+DQ  Q  +
Sbjct: 1   MPVKWVLHWQPNQGSTVSSQILNEATQCVESINGVKEGRWKATLNYYKPMLKDQANQ-LE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLG 120
           FPRDFLGISL +QPNKYY IIR QRIVLEAD SIQ IMEKLQSYK +VAL F+GFQYQLG
Sbjct: 61  FPRDFLGISLADQPNKYYFIIRTQRIVLEADSSIQLIMEKLQSYKSKVALYFDGFQYQLG 120

Query: 121 DFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DF+LRVGKVVP HSEN+RGIVME+EYLPISSMEK+++VMEEF E+W EA++K SLPG+F+
Sbjct: 121 DFRLRVGKVVPTHSENVRGIVMEVEYLPISSMEKAQKVMEEFLEIWNEALAKRSLPGKFV 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQAVQ 220
           +I+ NF E+GL D YT QHTAV+YA VMA +IATVQAV+
Sbjct: 181 NIDLNFGEFGLGDIYTPQHTAVRYALVMAHMIATVQAVR 218

[Close]

BLAST of Tan0021329 vs. TAIR 10
Match: AT4G09070.1 (TATA-binding related factor (TRF) of subunit 20 of Mediator complex )

HSP 1 Score: 305.8 bits (782), Expect = 2.8e-83
Identity = 144/217 (66.36%), Postives = 182/217 (83.87%), Query Frame = 0


Query: 1   MPLKWVLHWQPNAGSTVNSQILNEVSQCVESIHGVKGGKWKATLTFYKPILRDQNLQAAD 60
           MP+KW+LHWQPN GST +SQILNEV+Q +ES++GVK G WKATL +YKP+L+DQ  Q A+
Sbjct: 1   MPVKWLLHWQPNQGSTFSSQILNEVTQSIESLNGVKEGTWKATLNYYKPMLQDQANQ-AE 60

Query: 61  FPRDFLGISLPEQPNKYYLIIRGQRIVLEADFSIQTIMEKLQSYKLRVALNFEGFQYQLG 120
           FPR+F+GISLPE+P+KYY +IR QRIV+EAD SIQ IME LQSYK +++  FEG +YQLG
Sbjct: 61  FPREFVGISLPEEPDKYYFVIRSQRIVVEADSSIQMIMESLQSYKCKLSFYFEGLEYQLG 120

Query: 121 DFQLRVGKVVPNHSENLRGIVMEIEYLPISSMEKSRQVMEEFFEVWQEAMSKSSLPGRFM 180
           DF+LRVGKVVP H+E +RG+VME+EYLPISSM  ++++MEEF E+WQEAMSK SLPG+F+
Sbjct: 121 DFRLRVGKVVPTHAETIRGVVMEVEYLPISSMGMAKKLMEEFLEIWQEAMSKRSLPGKFV 180

Query: 181 HIEPNFAEYGLSDYYTSQHTAVQYANVMAQLIATVQA 218
           + E NF ++GL D YT QHTAV YA  MA L+A +QA
Sbjct: 181 NKELNFEKFGLGDNYTPQHTAVGYAFFMANLMAAIQA 216

[Close]

BLAST of Tan0021329 vs. TAIR 10
Match: AT2G28020.1 (BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of subunit 20 of Mediator complex (TAIR:AT2G28230.1); Has 37 Blast hits to 37 proteins in 13 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 37; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 83.2 bits (204), Expect = 2.9e-16
Identity = 43/68 (63.24%), Postives = 55/68 (80.88%), Query Frame = 0


Query: 152 MEKSRQVMEEFFEVWQEAMSKSSLPGRFMHIEPNFAEYGLSDYYTSQHTAVQ-YANVMAQ 211
           ME +R+VMEEF E+W EA++K SLPG+F++ + NF E+GL D YT QHTAV+ YA VMA 
Sbjct: 1   MEIARKVMEEFLEIWNEALAKRSLPGKFVNKDLNFGEFGLGDNYTPQHTAVRNYALVMAH 60

Query: 212 LIATVQAV 219
           +IATVQAV
Sbjct: 61  MIATVQAV 68

The following BLAST results are available for this feature:

BLAST of Tan0021329 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NPF4	7.1e-92	74.89	Mediator of RNA polymerase II transcription subunit 20a OS=Arabidopsis thaliana ...	[more]
Q9M0S0	3.9e-82	66.36	Mediator of RNA polymerase II transcription subunit 20b OS=Arabidopsis thaliana ...	[more]
Q54FL3	2.6e-09	28.43	Putative mediator of RNA polymerase II transcription subunit 20 OS=Dictyostelium...	[more]

BLAST of Tan0021329 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889134.1	1.5e-118	96.41	mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Beninca...	[more]
XP_022946745.1	6.1e-117	94.62	mediator of RNA polymerase II transcription subunit 20a-like [Cucurbita moschata...	[more]
XP_004141069.1	1.4e-116	95.07	mediator of RNA polymerase II transcription subunit 20a [Cucumis sativus] >KGN60...	[more]
XP_008459711.1	4.4e-115	93.27	PREDICTED: mediator of RNA polymerase II transcription subunit 20a-like isoform ...	[more]
KAA0061469.1	4.4e-115	93.27	mediator of RNA polymerase II transcription subunit 20a-like isoform X1 [Cucumis...	[more]

BLAST of Tan0021329 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KEU1	3.0e-117	94.62	Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita maxima OX=36...	[more]
A0A6J1G4U3	3.0e-117	94.62	Mediator of RNA polymerase II transcription subunit 20 OS=Cucurbita moschata OX=...	[more]
A0A0A0LHQ1	6.6e-117	95.07	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis sativus OX=365...	[more]
A0A5D3CH02	2.1e-115	93.27	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo var. maku...	[more]
A0A1S3CAW6	2.1e-115	93.27	Mediator of RNA polymerase II transcription subunit 20 OS=Cucumis melo OX=3656 G...	[more]

BLAST of Tan0021329 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28230.1	5.1e-93	74.89	TATA-binding related factor (TRF) of subunit 20 of Mediator complex	[more]
AT4G09070.1	2.8e-83	66.36	TATA-binding related factor (TRF) of subunit 20 of Mediator complex	[more]
AT2G28020.1	2.9e-16	63.24	BEST Arabidopsis thaliana protein match is: TATA-binding related factor (TRF) of...	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013921	Mediator complex, subunit Med20	PFAM	PF08612	Med20	coord: 1..208 e-value: 3.6E-45 score: 154.1
IPR013921	Mediator complex, subunit Med20	PANTHER	PTHR12465	UBIQUITIN SPECIFIC PROTEASE HOMOLOG 49	coord: 6..217
None	No IPR available	PANTHER	PTHR12465:SF0	MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 20	coord: 6..217

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0021329.1	Tan0021329.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045893	positive regulation of transcription, DNA-templated
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0016592	mediator complex
molecular_function	GO:0003713	transcription coactivator activity
molecular_function	GO:0003712	transcription coregulator activity