Tan0021276 (gene) Snake gourd v1

Overview


Name	Tan0021276
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Cystatin domain-containing protein
Location	LG03: 2025502 .. 2025959 (-)
RNA-Seq Expression	Tan0021276
Synteny	Tan0021276

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGGTTTAAAGGAGAGTTATAAATAGTGAAGTTGTGTTTGAATATTTCATACAAATCAAGTATTTTTCAATGAGTAATGTGGAAGCAGTTGAGGGTAATCCATTACCGATTAAGGATATCGATGCGCCGCAAGTGCAAGAAATTGGAAGTTTTGCCGTGAATGAATATAACAAGAAAGAAGGGGCGAATTTGAAGTTCAAACGCGTAGTGAATGGTTTGAGAACAACTCTTTTGCTTGCGGGTTTTCGTACATCAACAACATTTGATATTGTTGTGGAGGCCGAAAACAATGATGGAATTAATTGGACATATGTGGCTAAAGTCTCACATGTTACCGGATTCTTTCCCCCTACTTCTCTCCAGTCCTTTCAACCTATCCTTAAATACAACATCTAATACTTCAAATCCTGTCTTTTATATAATATCAATAAAATAATATCAATTATACTTATCTATTA

mRNA sequence

GAGGTTTAAAGGAGAGTTATAAATAGTGAAGTTGTGTTTGAATATTTCATACAAATCAAGTATTTTTCAATGAGTAATGTGGAAGCAGTTGAGGGTAATCCATTACCGATTAAGGATATCGATGCGCCGCAAGTGCAAGAAATTGGAAGTTTTGCCGTGAATGAATATAACAAGAAAGAAGGGGCGAATTTGAAGTTCAAACGCGTAGTGAATGGTTTGAGAACAACTCTTTTGCTTGCGGGTTTTCGTACATCAACAACATTTGATATTGTTGTGGAGGCCGAAAACAATGATGGAATTAATTGGACATATGTGGCTAAAGTCTCACATGTTACCGGATTCTTTCCCCCTACTTCTCTCCAGTCCTTTCAACCTATCCTTAAATACAACATCTAATACTTCAAATCCTGTCTTTTATATAATATCAATAAAATAATATCAATTATACTTATCTATTA

Coding sequence (CDS)

ATGAGTAATGTGGAAGCAGTTGAGGGTAATCCATTACCGATTAAGGATATCGATGCGCCGCAAGTGCAAGAAATTGGAAGTTTTGCCGTGAATGAATATAACAAGAAAGAAGGGGCGAATTTGAAGTTCAAACGCGTAGTGAATGGTTTGAGAACAACTCTTTTGCTTGCGGGTTTTCGTACATCAACAACATTTGATATTGTTGTGGAGGCCGAAAACAATGATGGAATTAATTGGACATATGTGGCTAAAGTCTCACATGTTACCGGATTCTTTCCCCCTACTTCTCTCCAGTCCTTTCAACCTATCCTTAAATACAACATCTAA

Protein sequence

MSNVEAVEGNPLPIKDIDAPQVQEIGSFAVNEYNKKEGANLKFKRVVNGLRTTLLLAGFRTSTTFDIVVEAENNDGINWTYVAKVSHVTGFFPPTSLQSFQPILKYNI

Homology

[Close]

BLAST of Tan0021276 vs. ExPASy Swiss-Prot
Match: Q41916 (Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 3.4e-07
Identity = 31/73 (42.47%), Postives = 47/73 (64.38%), Query Frame = 0


Query: 13 PIKDIDAPQVQEIGSFAVNEYNKKEGANLKFKRVVNGLRTTLLLAGFRTSTTFDIVVEAE 72
          PI ++  PQV EIG FAV+EYNK+  + LKF+ VV+G   T +++G    T + + V A 
Sbjct: 33 PISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSG--ETQVVSG----TNYRLKVAAN 92

Query: 73 NNDGINWTYVAKV 86
          + DG++  Y+A V
Sbjct: 93 DGDGVSKNYLAIV 99

[Close]

BLAST of Tan0021276 vs. NCBI nr
Match: KAE8649716.1 (hypothetical protein Csa_012476 [Cucumis sativus])

HSP 1 Score: 86.7 bits (213), Expect = 1.4e-13
Identity = 51/103 (49.51%), Postives = 64/103 (62.14%), Query Frame = 0


Query: 13  PIKDIDAPQVQEIGSFAVNEYNKKEGANLKFKRVVNGLRTTLLLAGF------RTSTTFD 72
           PIKDI+ PQVQEIG+  V+++NKK G NLKF RVVNG     L AGF         T ++
Sbjct: 9   PIKDINDPQVQEIGNLTVDDHNKKTGENLKFVRVVNG-----LFAGFVDPFSPLGGTVYE 68

Query: 73  IVVEAENNDGINWTYVAKVSHVTGFFP---PTSLQSFQPILKY 107
           +V+EA N   INWTY  KVS  T   P     + QSF+P+L+Y
Sbjct: 69  LVLEAINAGKINWTYKVKVSASTTIRPLGVVYNFQSFEPVLQY 106

[Close]

BLAST of Tan0021276 vs. NCBI nr
Match: KGN54823.1 (hypothetical protein Csa_012607 [Cucumis sativus])

HSP 1 Score: 77.4 bits (189), Expect = 8.4e-11
Identity = 42/96 (43.75%), Postives = 57/96 (59.38%), Query Frame = 0


Query: 13  PIKDIDAPQVQEIGSFAV-NEYNKKEGANLKFKRVVNGLRTTLLLA-GFRTSTTFDIVVE 72
           PI DI+ P VQ +G  AV N Y+K  G  LKF RVVNGL++   +  GF     + +V+E
Sbjct: 12  PIIDINDPHVQALGELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGPGFTEVILYHLVLE 71

Query: 73  AENNDGINWTYVAKVSHVTGFFPPTSLQSFQPILKY 107
           A+ N+ INWTY  K+  V+G        SF+P+L Y
Sbjct: 72  AKTNEEINWTYATKLEEVSGGLIRHVFLSFEPVLPY 107

[Close]

BLAST of Tan0021276 vs. NCBI nr
Match: XP_008442277.1 (PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa] >TYK05422.1 cysteine proteinase inhibitor A-like [Cucumis melo var. makuwa])

HSP 1 Score: 74.7 bits (182), Expect = 5.4e-10
Identity = 45/104 (43.27%), Postives = 62/104 (59.62%), Query Frame = 0


Query: 13  PIKDI-DAPQVQEIGSFAVNEYNKKEGANLKFKRVVNGLRTTLL-----LAGFRTSTTFD 72
           PI+DI D  +VQ++G+  V+++NKK G NLKF RVVNGL  +       L G      ++
Sbjct: 9   PIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG----VLYE 68

Query: 73  IVVEAENNDGINWTYVAKVSHVTGFFPP----TSLQSFQPILKY 107
           +V+EA     INWTY A+VS      PP     S QSF+P+L+Y
Sbjct: 69  LVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEY 108

[Close]

BLAST of Tan0021276 vs. NCBI nr
Match: KAA0040521.1 (Cystatin [Cucumis melo var. makuwa] >TYK05563.1 Cystatin [Cucumis melo var. makuwa])

HSP 1 Score: 70.9 bits (172), Expect = 7.8e-09
Identity = 37/86 (43.02%), Postives = 56/86 (65.12%), Query Frame = 0


Query: 1  MSNVEAVEGNPLPIKDIDAPQVQEIGSFAVNEYNKKEGANLKFKRVVNGLRTTLLLAGFR 60
          MS+ +    +P+PIKD+D+ ++Q +G FAV E+N+++G NL+F +V+NG        G R
Sbjct: 1  MSSEDLSNLDPVPIKDVDSEEIQGLGEFAVREHNEEKGENLEFVKVINGFE-----QGRR 60

Query: 61 -TSTTFDIVVEAENNDGINWTYVAKV 86
               F IV+ A+N +GI WTY AKV
Sbjct: 61 GVRIIFTIVLSAKNCNGIIWTYKAKV 81

[Close]

BLAST of Tan0021276 vs. NCBI nr
Match: XP_038891087.1 (uncharacterized protein LOC120080488 isoform X2 [Benincasa hispida])

HSP 1 Score: 68.2 bits (165), Expect = 5.1e-08
Identity = 35/76 (46.05%), Postives = 51/76 (67.11%), Query Frame = 0


Query: 10 NPLPIKDIDAPQVQEIGSFAVNEYNKKEGANLKFKRVVNGLRTTLLLAGFRT-STTFDIV 69
          +P+PIKD+ + ++Q  GSFAV E+N+  G  L+F+RV+NGL       G R+    F +V
Sbjct: 13 DPVPIKDVYSEEIQGCGSFAVREHNETTGEKLEFERVINGLE-----QGRRSIRIVFTLV 72

Query: 70 VEAENNDGINWTYVAK 85
          + A+N+ GINWTY AK
Sbjct: 73 LVAKNSCGINWTYTAK 83

[Close]

BLAST of Tan0021276 vs. ExPASy TrEMBL
Match: A0A0A0KZ98 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.0e-11
Identity = 42/96 (43.75%), Postives = 57/96 (59.38%), Query Frame = 0


Query: 13  PIKDIDAPQVQEIGSFAV-NEYNKKEGANLKFKRVVNGLRTTLLLA-GFRTSTTFDIVVE 72
           PI DI+ P VQ +G  AV N Y+K  G  LKF RVVNGL++   +  GF     + +V+E
Sbjct: 12  PIIDINDPHVQALGELAVENLYSKNSGEKLKFVRVVNGLKSDKYIGPGFTEVILYHLVLE 71

Query: 73  AENNDGINWTYVAKVSHVTGFFPPTSLQSFQPILKY 107
           A+ N+ INWTY  K+  V+G        SF+P+L Y
Sbjct: 72  AKTNEEINWTYATKLEEVSGGLIRHVFLSFEPVLPY 107

[Close]

BLAST of Tan0021276 vs. ExPASy TrEMBL
Match: A0A5A7TJK0 (Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold83G001140 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.6e-10
Identity = 45/104 (43.27%), Postives = 62/104 (59.62%), Query Frame = 0


Query: 13  PIKDI-DAPQVQEIGSFAVNEYNKKEGANLKFKRVVNGLRTTLL-----LAGFRTSTTFD 72
           PI+DI D  +VQ++G+  V+++NKK G NLKF RVVNGL  +       L G      ++
Sbjct: 9   PIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG----VLYE 68

Query: 73  IVVEAENNDGINWTYVAKVSHVTGFFPP----TSLQSFQPILKY 107
           +V+EA     INWTY A+VS      PP     S QSF+P+L+Y
Sbjct: 69  LVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEY 108

[Close]

BLAST of Tan0021276 vs. ExPASy TrEMBL
Match: A0A1S3B5B9 (cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 2.6e-10
Identity = 45/104 (43.27%), Postives = 62/104 (59.62%), Query Frame = 0


Query: 13  PIKDI-DAPQVQEIGSFAVNEYNKKEGANLKFKRVVNGLRTTLL-----LAGFRTSTTFD 72
           PI+DI D  +VQ++G+  V+++NKK G NLKF RVVNGL  +       L G      ++
Sbjct: 9   PIEDINDNLEVQDLGNLTVDDHNKKTGENLKFVRVVNGLAASFSDPISPLGG----VLYE 68

Query: 73  IVVEAENNDGINWTYVAKVSHVTGFFPP----TSLQSFQPILKY 107
           +V+EA     INWTY A+VS      PP     S QSF+P+L+Y
Sbjct: 69  LVLEAITTSKINWTYKARVSKSKAITPPFHMLYSFQSFKPVLEY 108

[Close]

BLAST of Tan0021276 vs. ExPASy TrEMBL
Match: A0A0A0L191 (Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 1.3e-09
Identity = 42/108 (38.89%), Postives = 61/108 (56.48%), Query Frame = 0


Query: 3   NVEAVEGNPLPIKDIDAPQVQEIGSFAVNEYNK-KEGANLKFKRVVNGLRTTLLLA-GFR 62
           ++E +     PI DI+ P VQ IG  AV +++    G  LKF RVVNGL++   +  GF 
Sbjct: 2   SIEEIHHRLKPIIDINDPHVQIIGKLAVEDHDSHNPGEKLKFVRVVNGLKSDKYIGPGFT 61

Query: 63  TSTTFDIVVEAENNDGINWTYVAKVSHV-TGFFPPTSLQSFQPILKYN 108
               + +V+EA+ N+ INWTY  K+  V +G        SF+P+L YN
Sbjct: 62  EVILYHLVLEAKTNEEINWTYATKLEKVYSGCLIRHVFLSFEPVLPYN 109

[Close]

BLAST of Tan0021276 vs. ExPASy TrEMBL
Match: A0A5A7TFU6 (Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00010 PE=4 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 3.8e-09
Identity = 37/86 (43.02%), Postives = 56/86 (65.12%), Query Frame = 0


Query: 1  MSNVEAVEGNPLPIKDIDAPQVQEIGSFAVNEYNKKEGANLKFKRVVNGLRTTLLLAGFR 60
          MS+ +    +P+PIKD+D+ ++Q +G FAV E+N+++G NL+F +V+NG        G R
Sbjct: 1  MSSEDLSNLDPVPIKDVDSEEIQGLGEFAVREHNEEKGENLEFVKVINGFE-----QGRR 60

Query: 61 -TSTTFDIVVEAENNDGINWTYVAKV 86
               F IV+ A+N +GI WTY AKV
Sbjct: 61 GVRIIFTIVLSAKNCNGIIWTYKAKV 81

[Close]

BLAST of Tan0021276 vs. TAIR 10
Match: AT5G47550.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 55.8 bits (133), Expect = 2.4e-08
Identity = 31/73 (42.47%), Postives = 47/73 (64.38%), Query Frame = 0


Query: 13 PIKDIDAPQVQEIGSFAVNEYNKKEGANLKFKRVVNGLRTTLLLAGFRTSTTFDIVVEAE 72
          PI ++  PQV EIG FAV+EYNK+  + LKF+ VV+G   T +++G    T + + V A 
Sbjct: 33 PISNVTDPQVVEIGEFAVSEYNKRSESGLKFETVVSG--ETQVVSG----TNYRLKVAAN 92

Query: 73 NNDGINWTYVAKV 86
          + DG++  Y+A V
Sbjct: 93 DGDGVSKNYLAIV 99

[Close]

BLAST of Tan0021276 vs. TAIR 10
Match: AT4G16500.1 (Cystatin/monellin superfamily protein )

HSP 1 Score: 43.5 bits (101), Expect = 1.2e-04
Identity = 29/95 (30.53%), Postives = 50/95 (52.63%), Query Frame = 0


Query: 9   GNPLPIKDIDAPQVQEIGSFAVNEYNKKEGANLKFKRVVNGLRTTLLLAGFRTSTTFDIV 68
           G+  PIK++  P V  +  +A+ E+NK+    L F +VV G  TT +++G    T +D+ 
Sbjct: 30  GSRKPIKNVSDPDVVAVAKYAIEEHNKESKEKLVFVKVVEG--TTQVVSG----TKYDLK 89

Query: 69  VEAENNDGINWTYVAKVSHVTGFFPPTSLQSFQPI 104
           + A++  G    Y A V     +    SL+SF+ +
Sbjct: 90  IAAKDGGGKIKNYEAVVVEKL-WLHSKSLESFKAL 117

The following BLAST results are available for this feature:

BLAST of Tan0021276 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41916	3.4e-07	42.47	Cysteine proteinase inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=CYS5 PE=2 SV=...	[more]

BLAST of Tan0021276 vs. NCBI nr


Match Name	E-value	Identity	Description
KAE8649716.1	1.4e-13	49.51	hypothetical protein Csa_012476 [Cucumis sativus]	[more]
KGN54823.1	8.4e-11	43.75	hypothetical protein Csa_012607 [Cucumis sativus]	[more]
XP_008442277.1	5.4e-10	43.27	PREDICTED: cysteine proteinase inhibitor A-like [Cucumis melo] >KAA0041897.1 cys...	[more]
KAA0040521.1	7.8e-09	43.02	Cystatin [Cucumis melo var. makuwa] >TYK05563.1 Cystatin [Cucumis melo var. maku...	[more]
XP_038891087.1	5.1e-08	46.05	uncharacterized protein LOC120080488 isoform X2 [Benincasa hispida]	[more]

BLAST of Tan0021276 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KZ98	4.0e-11	43.75	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G508510 PE=4 SV=1	[more]
A0A5A7TJK0	2.6e-10	43.27	Cysteine proteinase inhibitor A-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3B5B9	2.6e-10	43.27	cysteine proteinase inhibitor A-like OS=Cucumis melo OX=3656 GN=LOC103486185 PE=...	[more]
A0A0A0L191	1.3e-09	38.89	Cystatin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G508520 PE...	[more]
A0A5A7TFU6	3.8e-09	43.02	Cystatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G00010 PE=4 S...	[more]

BLAST of Tan0021276 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G47550.1	2.4e-08	42.47	Cystatin/monellin superfamily protein	[more]
AT4G16500.1	1.2e-04	30.53	Cystatin/monellin superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 7..107 e-value: 3.9E-14 score: 54.5
None	No IPR available	PANTHER	PTHR47116:SF5	PHLOEM FILAMENT PROTEIN	coord: 10..104
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 10..103
IPR000010	Cystatin domain	PFAM	PF16845	SQAPI	coord: 14..102 e-value: 8.4E-14 score: 51.7
IPR000010	Cystatin domain	CDD	cd00042	CY	coord: 9..48 e-value: 2.45918E-5 score: 38.224
IPR027214	Cystatin	PANTHER	PTHR47116	PHLOEM FILAMENT PROTEIN	coord: 10..104

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0021276.1	Tan0021276.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity