Tan0020852 (gene) Snake gourd v1

Overview


Name	Tan0020852
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Kunitz-type trypsin inhibitor
Location	LG08: 5141934 .. 5143788 (-)
RNA-Seq Expression	Tan0020852
Synteny	Tan0020852

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTAATGGAGAAAATCAGATAAGCTAACAAAATGGACTTTTGATCATTCTTGTCTTCTTCAAGCTGGCCATAAATAGCACTACACACATTGACATCCCCAAAACCATCAATTTTTCAGAACAATTTAAGTCTATAGTTGAAGAATTAGAAGATGATGTTGCATAAACCACTTGCCATCTTCAGCTACCTATGCATTTTCATGGCTATGTCCTCTACTGCCCAATCCGATGGTCCTCCGGTGCTCGACACCGAGGGCCGACCTCTGCAGCGCGATGTCGAGTACTTCGTCAAGCCCGCCATCACCGATGTTGCTGGTAATCTCACCCTAGTAACCCGCAAAGGTGATCAATGCCCGTTTCACGTTGGTCAAGTACCACTTCTTTCACAAGATATAGGTATTAAACCATAACTAAGCTCGACAAAAGTTTAATTTCATATATATATATATATATATATATATTAGGGGTGTTGAAAAAACCCGGTGATCCAAACAACTTGATCAGTACCCAACTTATATAGTTTGGGACGTTGGGTTATCTACTCATTTGAGTTAGACCGAGTTTAAATCAATAAAAATTTATGAGCTGAGTTGGTTGATGAGTTCACCTAAATTTTAAAAATGATTTTTTTACATACTAGGCTAGCTACTATACAATTACATATACCTGTAATTATTTTATTTTTATTTTATTTCATAACTATTTTTTAATTATTTATTCTTTAATAACTCTTAAAAATACATTTTTAAATCTTTAGGGTGTAATTTTTTATCATATAAATTGAAACTAAGTGGCTAATTTAATTCAATGTTTGAAAAACAAAATAAATAGATGATTTACATATTAACATTTTACTTTTTCTTAAAACCAAAATTTTAAATAAATGACTCAAACAACTCGAATCAACCAAACCTAAAAATGTTTCATGGTTGGGTTGGTTGGGTTCATTATTTAATAAGGATAAGAATTAGTTGGGTTGAAGAAATTTATAACTTGAACAATTAGATTGAGTCTAAAAAGTATCTCAACCCCAACCCAATCCAACTCATGTGAACACCCCTAATATATATATATATATATTACCTACATATATATACATACATACACACATTAAAACTAAAAATTTTGAATGGATAGGTTATGATAGGTTTAATTCTTAGCTTATATATATATTTTCTTAACAGGTTTTGGGGTCACCTTCACACCCTATCTAGAGGGTGAAGATACAATAAGGGAAGGTAGGGATTTGAAGGTTGTGTTTCAGGCGTTCTCAATTTGCATAACGTCGACTGCATGGAGAGTTGGGGAGACAGAGCCTAACACCGGAAGGAGGTTTGTAGAGACTGGGAGCGACGACACTGCCACTGGGTACTTCAAGATCGATAAGGATAATAATTTCGGAGTTTATAACATAGGATGGTGTCCCTCAGATGTCCCCTTTAAAGGAAGGCCAAGGTGTGGAAGGGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTGTTGGCTTTGGATGGCGCTGCGTTTCCTTTTGAGTTTGTGAGAGCTTGAGACAAGAAAATTACATGACTTTATCTTGCAAGAGAAAATGCTTCCAATTTGTTGCAATTGCATTAAATTAGATTAAATAATGAAACTCTGGTAAGAATTTACTTTATGTGATTATAAAAAAAAGTGTTACTTTATGTTGTTTTATTGTTATGATTAATGTGATTTAGCATTTGGTTTACGTTTACCATCGAATATATATGTGTGTGTTAAGGCTTATGAGTCCGTCATTTAATTGGTATGTTAGTTCTTATTAATATATGAAACAGATATCTAAAACAAATTATGTAGTTTGATAGAGGGTAGTGAGCAAC

mRNA sequence

GTAATGGAGAAAATCAGATAAGCTAACAAAATGGACTTTTGATCATTCTTGTCTTCTTCAAGCTGGCCATAAATAGCACTACACACATTGACATCCCCAAAACCATCAATTTTTCAGAACAATTTAAGTCTATAGTTGAAGAATTAGAAGATGATGTTGCATAAACCACTTGCCATCTTCAGCTACCTATGCATTTTCATGGCTATGTCCTCTACTGCCCAATCCGATGGTCCTCCGGTGCTCGACACCGAGGGCCGACCTCTGCAGCGCGATGTCGAGTACTTCGTCAAGCCCGCCATCACCGATGTTGCTGGTAATCTCACCCTAGTAACCCGCAAAGGTGATCAATGCCCGTTTCACGTTGGTCAAGTACCACTTCTTTCACAAGATATAGGTTTTGGGGTCACCTTCACACCCTATCTAGAGGGTGAAGATACAATAAGGGAAGGTAGGGATTTGAAGGTTGTGTTTCAGGCGTTCTCAATTTGCATAACGTCGACTGCATGGAGAGTTGGGGAGACAGAGCCTAACACCGGAAGGAGGTTTGTAGAGACTGGGAGCGACGACACTGCCACTGGGTACTTCAAGATCGATAAGGATAATAATTTCGGAGTTTATAACATAGGATGGTGTCCCTCAGATGTCCCCTTTAAAGGAAGGCCAAGGTGTGGAAGGGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTGTTGGCTTTGGATGGCGCTGCGTTTCCTTTTGAGTTTGTGAGAGCTTGAGACAAGAAAATTACATGACTTTATCTTGCAAGAGAAAATGCTTCCAATTTGTTGCAATTGCATTAAATTAGATTAAATAATGAAACTCTGGTAAGAATTTACTTTATGTGATTATAAAAAAAAGTGTTACTTTATGTTGTTTTATTGTTATGATTAATGTGATTTAGCATTTGGTTTACGTTTACCATCGAATATATATGTGTGTGTTAAGGCTTATGAGTCCGTCATTTAATTGGTATGTTAGTTCTTATTAATATATGAAACAGATATCTAAAACAAATTATGTAGTTTGATAGAGGGTAGTGAGCAAC

Coding sequence (CDS)

ATGATGTTGCATAAACCACTTGCCATCTTCAGCTACCTATGCATTTTCATGGCTATGTCCTCTACTGCCCAATCCGATGGTCCTCCGGTGCTCGACACCGAGGGCCGACCTCTGCAGCGCGATGTCGAGTACTTCGTCAAGCCCGCCATCACCGATGTTGCTGGTAATCTCACCCTAGTAACCCGCAAAGGTGATCAATGCCCGTTTCACGTTGGTCAAGTACCACTTCTTTCACAAGATATAGGTTTTGGGGTCACCTTCACACCCTATCTAGAGGGTGAAGATACAATAAGGGAAGGTAGGGATTTGAAGGTTGTGTTTCAGGCGTTCTCAATTTGCATAACGTCGACTGCATGGAGAGTTGGGGAGACAGAGCCTAACACCGGAAGGAGGTTTGTAGAGACTGGGAGCGACGACACTGCCACTGGGTACTTCAAGATCGATAAGGATAATAATTTCGGAGTTTATAACATAGGATGGTGTCCCTCAGATGTCCCCTTTAAAGGAAGGCCAAGGTGTGGAAGGGCTGGAATTTTGATTGAGAAAGGTTTGAGGTTGTTGGCTTTGGATGGCGCTGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

Protein sequence

MMLHKPLAIFSYLCIFMAMSSTAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLVTRKGDQCPFHVGQVPLLSQDIGFGVTFTPYLEGEDTIREGRDLKVVFQAFSICITSTAWRVGETEPNTGRRFVETGSDDTATGYFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGILIEKGLRLLALDGAAFPFEFVRA

Homology

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BLAST of Tan0020852 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 8.2e-39
Identity = 82/175 (46.86%), Postives = 113/175 (64.57%), Query Frame = 0


Query: 30  VLDTEGRPLQRDVEYFVKPAITDVAGNLTLVTRKGDQCPFHVGQVPLLSQDIGFGVTFTP 89
           V+DT G P++ D +YF++PAIT   G+LTLVTR  + CPF+VG  P   Q  GF V  +P
Sbjct: 27  VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTR--NSCPFNVGLDPDAPQ--GFAVLLSP 86

Query: 90  YLEG--EDTIREGRDLKVVFQAFSICITSTAWRVGETEPNTGRRFVETGSDDTATG---- 149
           ++    ED +R GRDL+V+FQA + C  ST WR+GE +  TGRRF+ TG DD+  G    
Sbjct: 87  FVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDATTGRRFIITGRDDSTVGSYGN 146

Query: 150 YFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGILIEKGLRLLALDGAAFPFEF 199
           +F+I +  + G++NI WCP++V    +  CG  GI+ E G  LLALDG+A P  F
Sbjct: 147 FFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRENGKILLALDGSALPVAF 197

[Close]

BLAST of Tan0020852 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.9e-35
Identity = 80/200 (40.00%), Postives = 115/200 (57.50%), Query Frame = 0


Query: 5   KPLAIFSYLCIFMAMSSTAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLVTRKG 64
           + L I +++C+F+  ++ AQ     VLDT G P++ D EYF++P IT+  G  T+V+R  
Sbjct: 8   RTLIILAHVCLFITTTTIAQF----VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVSR-N 67

Query: 65  DQCPFHVGQVPLLSQDIGFGVTFTPYLEGE--DTIREGRDLKVVFQAFSICITSTAWRVG 124
           + CP HVG + L     G  V FTP+      D +R  RDL++ FQA S C+ ST WR+G
Sbjct: 68  ESCPLHVG-LELTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEWRLG 127

Query: 125 ETEPNTGRRFVETGSDDTATG----YFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGI 184
           E +  +GRR + TG+D    G    +F+I +    G+YNI WCP++V    +  CG   +
Sbjct: 128 EKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGTVDM 187

Query: 185 LIEKGLRLLALDGAAFPFEF 199
           L E G  LLALDG   P  F
Sbjct: 188 LNENGKILLALDGGPLPLVF 201

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BLAST of Tan0020852 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.0e-33
Identity = 79/195 (40.51%), Postives = 110/195 (56.41%), Query Frame = 0


Query: 9   IFSYLCIFMAMSSTAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLVTRKGDQCP 68
           I +++ + MA +S AQ     V+DT G P++ D EYF++PAIT   G  TLVT  G  CP
Sbjct: 10  ILAHVWLLMATTSIAQF----VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVTGNG-PCP 69

Query: 69  FHVGQVPLLSQDIGFGVTFTPYL--EGEDTIREGRDLKVVFQAFSICITSTAWRVGETEP 128
            HVG +      +G  V FTP+     +D +R  RDL+V F   + C  ST WR+GE + 
Sbjct: 70  LHVG-LDNTEGTLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDWRLGEKDA 129

Query: 129 NTGRRFVETGSDDTA---TGYFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGILIEKG 188
            +GRR + TG D+ A     +F+I +    G YNI WCP++     + +CG  G++ E G
Sbjct: 130 TSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTVGVIRENG 189

Query: 189 LRLLALDGAAFPFEF 199
             LLALDG A P  F
Sbjct: 190 KNLLALDGDALPVVF 198

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BLAST of Tan0020852 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 95.1 bits (235), Expect = 9.5e-19
Identity = 66/190 (34.74%), Postives = 92/190 (48.42%), Query Frame = 0


Query: 21  STAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTL--VTRKGD-QCPFHVGQVPLL 80
           S A S   PVLD +G  L+    Y++ P + D  G LT+   T  G   CP  V Q    
Sbjct: 27  SAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQT-RK 86

Query: 81  SQDIGFGVTFTPYLEGEDTIREGRDLKVVFQAFSIC--ITSTAWRVGETEPNTGRRFVET 140
             D    + F P    ED +R   DL + F AF  C   +ST WR+ + + +TG+ FV  
Sbjct: 87  EVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTI 146

Query: 141 G------SDDTATGYFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGILI-EKGLRLLA 199
           G        +T + +FKI++    G Y + +CP+ V    + +CG  GI I +KG R LA
Sbjct: 147 GGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPT-VCGSCKVKCGDVGIYIDQKGRRRLA 206

[Close]

BLAST of Tan0020852 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 80.9 bits (198), Expect = 1.8e-14
Identity = 55/138 (39.86%), Postives = 69/138 (50.00%), Query Frame = 0


Query: 3   LHKPLAIFSYLCIFMAMSSTAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLVTR 62
           L  PL + S L I  + S+      PPV DTEG  L  D  Y+V PA     G LT+  R
Sbjct: 4   LRLPLILLSLLAISFSCSAAP----PPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPR 63

Query: 63  KGDQCPFHVGQVPLLSQDIGFGVTFTPY----LEGEDTIREGRDLKVVFQAFSICITSTA 122
               CP  V Q     +  GF V FTP+       + TIR   D+++ F A +IC+ ST 
Sbjct: 64  V-LPCPLLVAQ-ETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTE 123

Query: 123 WRVGETEPNTGRRFVETG 137
           W VG+ EP TG R V TG
Sbjct: 124 WHVGD-EPLTGARRVVTG 134

[Close]

BLAST of Tan0020852 vs. NCBI nr
Match: XP_038888505.1 (kunitz type trypsin inhibitor 111-like [Benincasa hispida])

HSP 1 Score: 363.2 bits (931), Expect = 1.4e-96
Identity = 171/200 (85.50%), Postives = 185/200 (92.50%), Query Frame = 0


Query: 1   MMLHKPLAIFSYLCIFMAMSSTAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLV 60
           MM HK LAIFS LC FMA+SS AQ DGPPVLDTEGRPLQRDVEYF+KPAITDVAGNLTL+
Sbjct: 2   MMFHKSLAIFSSLCFFMAISSIAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLI 61

Query: 61  TRKGDQCPFHVGQVPLLSQDIGFGVTFTPYLEGEDTIREGRDLKVVFQAFSICITSTAWR 120
           TRKGDQCPF+VGQVPLLSQDIGFGVTFTPYL GEDTIREGRDLKVVFQAF+ICITST W+
Sbjct: 62  TRKGDQCPFYVGQVPLLSQDIGFGVTFTPYLAGEDTIREGRDLKVVFQAFTICITSTQWK 121

Query: 121 VGETEPNTGRRFVETGSDDTATGYFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGILI 180
           VGE +P TGRRFV+ G+DDTATGYF+IDK ++ GVYNIGWCPSDVP +GRPRCG AGILI
Sbjct: 122 VGEADPITGRRFVKLGNDDTATGYFRIDK-SDLGVYNIGWCPSDVPIRGRPRCGSAGILI 181

Query: 181 EKGLRLLALDGAAFPFEFVR 201
           EKGLRLLALDG+AFPFEFVR
Sbjct: 182 EKGLRLLALDGSAFPFEFVR 200

[Close]

BLAST of Tan0020852 vs. NCBI nr
Match: XP_022938134.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata])

HSP 1 Score: 362.8 bits (930), Expect = 1.9e-96
Identity = 174/201 (86.57%), Postives = 186/201 (92.54%), Query Frame = 0


Query: 1   MMLHKPLAIFSYLCIFMAMSSTAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLV 60
           MML K L  FSYLC+ MA+SS AQS  PPVLDTEGRPLQ +VEYF+KPA+TDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLL 60

Query: 61  TRKGDQCPFHVGQVPLLSQDIGFGVTFTPYLEGEDTIREGRDLKVVFQAFSICITSTAWR 120
           TRKGDQCPFHVGQVPL SQ+IGFGVTFTPYL GEDTIREGRDLKVVFQAFSICITSTAWR
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPNTGRRFVETGSDDTATGYFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGILI 180
           VGET+P TGRRFVETG DDTATGYF+ID+D NFGVYNIGWCPSDVP +GRPRCGRAGILI
Sbjct: 121 VGETDPTTGRRFVETGDDDTATGYFRIDRD-NFGVYNIGWCPSDVPIRGRPRCGRAGILI 180

Query: 181 EKGLRLLALDGAAFPFEFVRA 202
           EKGLRLLALDG+AFPFEFVRA
Sbjct: 181 EKGLRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Tan0020852 vs. NCBI nr
Match: KAG7021121.1 (hypothetical protein SDJN02_17809, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 361.7 bits (927), Expect = 4.1e-96
Identity = 173/200 (86.50%), Postives = 185/200 (92.50%), Query Frame = 0


Query: 2   MLHKPLAIFSYLCIFMAMSSTAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLVT 61
           ML K L  FSYLC+ MA+SS AQS  PPVLDTEGRPLQ +VEYF+KPA+TDVAGNLTL+T
Sbjct: 1   MLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLT 60

Query: 62  RKGDQCPFHVGQVPLLSQDIGFGVTFTPYLEGEDTIREGRDLKVVFQAFSICITSTAWRV 121
           RKGDQCPFHVGQVPL SQ+IGFGVTFTPYL GEDTIREGRDLKVVFQAFSICITSTAWRV
Sbjct: 61  RKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRV 120

Query: 122 GETEPNTGRRFVETGSDDTATGYFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGILIE 181
           GET+P TGRRFVETG DDTATGYF+ID+D NFGVYNIGWCPSDVP +GRPRCGRAGILIE
Sbjct: 121 GETDPTTGRRFVETGDDDTATGYFRIDRD-NFGVYNIGWCPSDVPIRGRPRCGRAGILIE 180

Query: 182 KGLRLLALDGAAFPFEFVRA 202
           KGLRLLALDG+AFPFEFVRA
Sbjct: 181 KGLRLLALDGSAFPFEFVRA 197

[Close]

BLAST of Tan0020852 vs. NCBI nr
Match: XP_023521354.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_023538197.1 alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360.9 bits (925), Expect = 7.0e-96
Identity = 173/201 (86.07%), Postives = 186/201 (92.54%), Query Frame = 0


Query: 1   MMLHKPLAIFSYLCIFMAMSSTAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLV 60
           MML K L  FSYLC+ MA+SS AQS  PPVLDTEGRPLQ +VEYF+KPAITDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAITDVAGNLTLL 60

Query: 61  TRKGDQCPFHVGQVPLLSQDIGFGVTFTPYLEGEDTIREGRDLKVVFQAFSICITSTAWR 120
           TRKGDQCPFHVGQVPL SQ+IGFGVTFTPYL GEDTIREGRDLKVVFQAFSICITSTAWR
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPNTGRRFVETGSDDTATGYFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGILI 180
           VGET+P TGRRFVETG +DTATGYF+ID+D NFG+YNIGWCPSDVP +GRPRCGRAGILI
Sbjct: 121 VGETDPTTGRRFVETGDNDTATGYFRIDRD-NFGIYNIGWCPSDVPIRGRPRCGRAGILI 180

Query: 181 EKGLRLLALDGAAFPFEFVRA 202
           EKGLRLLALDG+AFPFEFVRA
Sbjct: 181 EKGLRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Tan0020852 vs. NCBI nr
Match: XP_022965952.1 (kunitz trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 352.4 bits (903), Expect = 2.5e-93
Identity = 170/201 (84.58%), Postives = 181/201 (90.05%), Query Frame = 0


Query: 1   MMLHKPLAIFSYLCIFMAMSSTAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLV 60
           MML K L  FSYLC+ MA SS AQS  PPVLD EGRPL+ +VEYF+KPAITDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAASSIAQS--PPVLDIEGRPLEINVEYFIKPAITDVAGNLTLL 60

Query: 61  TRKGDQCPFHVGQVPLLSQDIGFGVTFTPYLEGEDTIREGRDLKVVFQAFSICITSTAWR 120
           TRKGDQCPFHVGQVPL SQ+IGFGVTFTPYL  ED IREGRDLKVVFQAFSICITSTAWR
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPNTGRRFVETGSDDTATGYFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGILI 180
           VGE +P TGRRFVETG DDTATGYF+ID+D NFGVYNIGWCPSDVP +GRPRCGRAGILI
Sbjct: 121 VGEADPTTGRRFVETGDDDTATGYFRIDRD-NFGVYNIGWCPSDVPIRGRPRCGRAGILI 180

Query: 181 EKGLRLLALDGAAFPFEFVRA 202
           EKGLRLLALDG+AFPFEFVRA
Sbjct: 181 EKGLRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Tan0020852 vs. ExPASy TrEMBL
Match: A0A6J1FD70 (alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111444311 PE=3 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 9.0e-97
Identity = 174/201 (86.57%), Postives = 186/201 (92.54%), Query Frame = 0


Query: 1   MMLHKPLAIFSYLCIFMAMSSTAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLV 60
           MML K L  FSYLC+ MA+SS AQS  PPVLDTEGRPLQ +VEYF+KPA+TDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLL 60

Query: 61  TRKGDQCPFHVGQVPLLSQDIGFGVTFTPYLEGEDTIREGRDLKVVFQAFSICITSTAWR 120
           TRKGDQCPFHVGQVPL SQ+IGFGVTFTPYL GEDTIREGRDLKVVFQAFSICITSTAWR
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPNTGRRFVETGSDDTATGYFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGILI 180
           VGET+P TGRRFVETG DDTATGYF+ID+D NFGVYNIGWCPSDVP +GRPRCGRAGILI
Sbjct: 121 VGETDPTTGRRFVETGDDDTATGYFRIDRD-NFGVYNIGWCPSDVPIRGRPRCGRAGILI 180

Query: 181 EKGLRLLALDGAAFPFEFVRA 202
           EKGLRLLALDG+AFPFEFVRA
Sbjct: 181 EKGLRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Tan0020852 vs. ExPASy TrEMBL
Match: A0A6J1HLP6 (kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.2e-93
Identity = 170/201 (84.58%), Postives = 181/201 (90.05%), Query Frame = 0


Query: 1   MMLHKPLAIFSYLCIFMAMSSTAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLV 60
           MML K L  FSYLC+ MA SS AQS  PPVLD EGRPL+ +VEYF+KPAITDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAASSIAQS--PPVLDIEGRPLEINVEYFIKPAITDVAGNLTLL 60

Query: 61  TRKGDQCPFHVGQVPLLSQDIGFGVTFTPYLEGEDTIREGRDLKVVFQAFSICITSTAWR 120
           TRKGDQCPFHVGQVPL SQ+IGFGVTFTPYL  ED IREGRDLKVVFQAFSICITSTAWR
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPNTGRRFVETGSDDTATGYFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGILI 180
           VGE +P TGRRFVETG DDTATGYF+ID+D NFGVYNIGWCPSDVP +GRPRCGRAGILI
Sbjct: 121 VGEADPTTGRRFVETGDDDTATGYFRIDRD-NFGVYNIGWCPSDVPIRGRPRCGRAGILI 180

Query: 181 EKGLRLLALDGAAFPFEFVRA 202
           EKGLRLLALDG+AFPFEFVRA
Sbjct: 181 EKGLRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Tan0020852 vs. ExPASy TrEMBL
Match: A0A0A0LGB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1)

HSP 1 Score: 350.5 bits (898), Expect = 4.6e-93
Identity = 164/201 (81.59%), Postives = 182/201 (90.55%), Query Frame = 0


Query: 1   MMLHKPL-AIFSYLCIFMAMSSTAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTL 60
           M  HK L A+FS+LC FMA+ STAQ DGPPVLDTEGRPLQRDVEYF+KPAITDVAGNLTL
Sbjct: 2   MRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 61

Query: 61  VTRKGDQCPFHVGQVPLLSQDIGFGVTFTPYLEGEDTIREGRDLKVVFQAFSICITSTAW 120
           +TRKGDQCPF+VGQVPLLSQ+IGFGVT TPYLEG+DTIREGRDLK VFQAF+ICIT T W
Sbjct: 62  ITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQW 121

Query: 121 RVGETEPNTGRRFVETGSDDTATGYFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGIL 180
           +VGE +P +GRRFV+ G+D+TATGYF+IDK +N GVYNIGWCPSDVPFKGRPRCG AGIL
Sbjct: 122 KVGEEDPKSGRRFVKLGNDNTATGYFRIDK-SNLGVYNIGWCPSDVPFKGRPRCGSAGIL 181

Query: 181 IEKGLRLLALDGAAFPFEFVR 201
           IEKG+R LALDG AFPFEFVR
Sbjct: 182 IEKGVRFLALDGPAFPFEFVR 201

[Close]

BLAST of Tan0020852 vs. ExPASy TrEMBL
Match: A0A6J1DBS1 (miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018865 PE=3 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 9.9e-88
Identity = 159/201 (79.10%), Postives = 179/201 (89.05%), Query Frame = 0


Query: 1   MMLHKPLAIFSYLCIFMAMSSTAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLV 60
           M+L KPLAIFS L +FMA+ STAQS  PPVLDT+GRPLQR VEYF+KPA+TDVAGNLTL+
Sbjct: 3   MLLQKPLAIFSCLFLFMAIYSTAQS--PPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLL 62

Query: 61  TRKGDQCPFHVGQVPLLSQDIGFGVTFTPYLEGEDTIREGRDLKVVFQAFSICITSTAWR 120
           TRKGD CPFHVGQVPL S DIG+ +    +L GEDTIREGRDLKVVFQAFSICITSTAWR
Sbjct: 63  TRKGDPCPFHVGQVPLRSTDIGYYILNLRFLAGEDTIREGRDLKVVFQAFSICITSTAWR 122

Query: 121 VGETEPNTGRRFVETGSDDTATGYFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGILI 180
           VGETEP TGRR V TG+DDTATG+F+IDK ++ GVYN+GWCPSDVP +GRPRCGRAGIL+
Sbjct: 123 VGETEPTTGRRLVATGNDDTATGFFRIDK-SDLGVYNLGWCPSDVPIRGRPRCGRAGILV 182

Query: 181 EKGLRLLALDGAAFPFEFVRA 202
           EKG+RLLALDG+AFPFEFVRA
Sbjct: 183 EKGVRLLALDGSAFPFEFVRA 200

[Close]

BLAST of Tan0020852 vs. ExPASy TrEMBL
Match: A0A5A7V7Y2 (Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00540 PE=3 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 3.8e-87
Identity = 158/202 (78.22%), Postives = 173/202 (85.64%), Query Frame = 0


Query: 1   MMLHKPL-AIFSYLCIFMAMSSTA-QSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLT 60
           M  HK L AIFS LC FMA+ STA Q DGPPVLDTEGRPLQRD EYF+KPAITDVAGNLT
Sbjct: 2   MRFHKSLFAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLT 61

Query: 61  LVTRKGDQCPFHVGQVPLLSQDIGFGVTFTPYLEGEDTIREGRDLKVVFQAFSICITSTA 120
           L+TRKGDQCPF+VGQVPL SQ+ GFGV+  PY EGEDTIREGRDLK VFQ F+ICIT T 
Sbjct: 62  LITRKGDQCPFYVGQVPLFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQ 121

Query: 121 WRVGETEPNTGRRFVETGSDDTATGYFKIDKDNNFGVYNIGWCPSDVPFKGRPRCGRAGI 180
           W+VGE +P TGRRFV+ G D+TATGYF+IDK ++ GVYNIGWCPSDVP KGRPRCG AGI
Sbjct: 122 WKVGEADPKTGRRFVKLGYDNTATGYFRIDK-SDLGVYNIGWCPSDVPIKGRPRCGSAGI 181

Query: 181 LIEKGLRLLALDGAAFPFEFVR 201
           LIEKG+R LALDG AFPFEFVR
Sbjct: 182 LIEKGVRFLALDGPAFPFEFVR 202

[Close]

BLAST of Tan0020852 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 49.7 bits (117), Expect = 3.2e-06
Identity = 41/154 (26.62%), Postives = 66/154 (42.86%), Query Frame = 0


Query: 15  IFMAMSSTAQSDGPPVLDTEGRPLQRDVEYFVKPAITDVAGNLTLVTRK---GDQCPFHV 74
           IF A S+   +    VLD  G P+Q +V+Y++ PA     G L    R     D C  ++
Sbjct: 17  IFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLC-LNL 76

Query: 75  GQVPLLSQDI-GFGVTFTPYLEGEDTIREGRDLKVVFQA-FSICITSTAWRVGETEPNTG 134
             V   S  + G  VTF+P       ++    L + F +   +C  S  WR+ +      
Sbjct: 77  DIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRI-DHSVQLR 136

Query: 135 RRFVETGSD-DTATGYFKIDKDNNFGVYNIGWCP 163
           + FV  G        +F+I +D +   Y + +CP
Sbjct: 137 KSFVSIGGQKGKGNSWFQIQEDGD--AYKLMYCP 166

The following BLAST results are available for this feature:

BLAST of Tan0020852 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV7	8.2e-39	46.86	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
G7LCV1	1.9e-35	40.00	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
A0A072TH68	1.0e-33	40.51	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
P13087	9.5e-19	34.74	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P29421	1.8e-14	39.86	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]

BLAST of Tan0020852 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888505.1	1.4e-96	85.50	kunitz type trypsin inhibitor 111-like [Benincasa hispida]	[more]
XP_022938134.1	1.9e-96	86.57	alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata]	[more]
KAG7021121.1	4.1e-96	86.50	hypothetical protein SDJN02_17809, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023521354.1	7.0e-96	86.07	alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_0235381...	[more]
XP_022965952.1	2.5e-93	84.58	kunitz trypsin inhibitor 1-like [Cucurbita maxima]	[more]

BLAST of Tan0020852 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FD70	9.0e-97	86.57	alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HLP6	1.2e-93	84.58	kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3...	[more]
A0A0A0LGB4	4.6e-93	81.59	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1	[more]
A0A6J1DBS1	9.9e-88	79.10	miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018865 PE=3 SV=1	[more]
A0A5A7V7Y2	3.8e-87	78.22	Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa O...	[more]

BLAST of Tan0020852 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73325.1	3.2e-06	26.62	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 29..201 e-value: 9.8E-24 score: 94.9
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 30..199 e-value: 1.2E-37 score: 129.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..201
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 30..46
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 29..200 e-value: 3.8689E-35 score: 120.14
None	No IPR available	GENE3D	2.80.10.50		coord: 27..201 e-value: 4.2E-39 score: 136.1
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 8..201
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 28..200

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0020852.1	Tan0020852.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0050896	response to stimulus
molecular_function	GO:0004866	endopeptidase inhibitor activity