Tan0020768 (gene) Snake gourd v1

Overview


Name	Tan0020768
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Thiamine thiazole synthase, chloroplastic
Location	LG01: 17852659 .. 17854740 (+)
RNA-Seq Expression	Tan0020768
Synteny	Tan0020768

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGGGTGCACCCCCAAGTATTTCTCTATAAAACCCCTCTTCCGTCCACCTTCTTCCCCATCCCATCTCCTCTGTTTTCCTCTGCTTGAAAATGGCCTCCATTGCCTCCACTCTTCCCACCAAGCTCCAAAGGCCCTCCTTACTCGATTCCTCTTTCCATGGCACCCCTTTGGCCCCTCCTCCTTCTCTCCGCCTCAAATCCTCCGCCGCCGCCTCTCTCTCCATCTCCGCCTCCGCCTCCGCCTCTCAACCGCCCTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATAGTCTCACGGGAGATGACCCGCCGCTACATGACCGACATGATCACTTACGCCGACACTGATGTTATCATCGTCGGTGCTGGCTCCGCCGGTCTCTCTTGCGCTTATGAGCTCAGCAAAGACCCCAATATCCGCATCGCCATCATCGAGCAGTCCGTCAGCCCCGGTGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTAATTATTTCATTTCTGACGCATAACGCCTCATTTATTCCTGTTTTTTTTTTCAAGCTTTAAATGCTCTGTTTTTTTTAAGAAAATACCCACCTAGTTTTTCACCTTCTAAGTCATGCATGCCGTAATAGGACTCGAGATTTCATCATTTTTTATCGGAGATCTGAAAGAAAGAAAGTTGAAACAAGTTTAGAGCATATTAATTTGGCACAAGCCCAAGAATTAAAATACTTGCAAGATTGGAAGATCTGATCTTTGAGACGGATGTATAATTTTCAAATCAGTTGAGATAGGTTCGGTGGGCTGGTTTTCGTTATTGTAGCATATTCAATCAGCAGCAGTTCTGATTCCAAAATCTTGAAGCGGATGGATTTGGGTGAAATTAATGTTGGATTCGTATTCTTTGACAGGTGGTACGGAAGCCAGCCCATAAATTCTTGGACGAGGTGGGTGTGGAGTACGACGAGCAAGATGACTACGTTGTAATCAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCAAAGCCCAACGTCAAGCTGTTCAACGCCGTCGCGGCGGAGGATTTGATCGTAAAGGGCGGCAGAGTCGGCGGCGTCGTCACCAACTGGGCGCTGGTGTCGATGAACCATGACACACAGTCCTGCATGGACCCCAATGTGATGGAGGCCAAGGTGGTGGTCAGCTCTTGCGGCCACGATGGGCCCTTCGGGGCCACCGGAGTCAAGCGCCTCAGGAGCATCGGCATGATTGATTCCATCCCCGGAATGAAGGCCCTCGACATGAACACGGCGGAGGACGCCATTGTTAGGCTTACCCGAGAGGTCGTGCCCGGCATGATCGTTACCGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGTAAAGAATTAAGTTTAAATTAGTAGAACTAAAAAACCCAATTTGGTAATCATTTGATTCCCGTATGATATATTTAATTTGGGATACAGGGGCCGACATTCGGAGCGATGATGATATCGGGGCAGAAGGCGGCGCAGTTGGCGTTGAAGTCGTTGGGGCGAGAAAATTGCATTCGAGAAGAGGTTTTGGTTGAAGAAGCAGAAATGCAACTGGCGGCGGGGGAGTCGCCGGAGGTTGCAGATGCTTGAATTGGTTTGATTTGGAAGAAAAATTTGCTTTTTTTGGGTTTGTTTGTTGTTTGTACTGTGTAGTGAGTTTTCAGTTTTCAGTTTTCAGTTTTCAGTGATGAATGGCTCTGTTCGTAGCATAGCACGAGGGAAAGGGTTAGGGTTTGGGGAAAAGACAAAATATCACTTCCCTCGTTTCTTTTCTCTCTTTTATTTTTTTTAATAAAAATGCGTTCCAATTCCAAGGACAAGTTATTCGGTGAGGCCCGTGGTAAGGGTAACCACTAAATCAACTTTTAATTTGATTATTTCTATACAGATACATATTATATGCAGTGTATATAAAAATTTAAAGCACTCAGGCTCCCTCTAAGCCTTAACAATCTGTCTTTTTCAGTTCTCTTCCTAAATTACTGAGTGAGACCGTCTCATGCTAAAATAAAATTAATGAA

mRNA sequence

GTGGGTGCACCCCCAAGTATTTCTCTATAAAACCCCTCTTCCGTCCACCTTCTTCCCCATCCCATCTCCTCTGTTTTCCTCTGCTTGAAAATGGCCTCCATTGCCTCCACTCTTCCCACCAAGCTCCAAAGGCCCTCCTTACTCGATTCCTCTTTCCATGGCACCCCTTTGGCCCCTCCTCCTTCTCTCCGCCTCAAATCCTCCGCCGCCGCCTCTCTCTCCATCTCCGCCTCCGCCTCCGCCTCTCAACCGCCCTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATAGTCTCACGGGAGATGACCCGCCGCTACATGACCGACATGATCACTTACGCCGACACTGATGTTATCATCGTCGGTGCTGGCTCCGCCGGTCTCTCTTGCGCTTATGAGCTCAGCAAAGACCCCAATATCCGCATCGCCATCATCGAGCAGTCCGTCAGCCCCGGTGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTGGTACGGAAGCCAGCCCATAAATTCTTGGACGAGGTGGGTGTGGAGTACGACGAGCAAGATGACTACGTTGTAATCAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCAAAGCCCAACGTCAAGCTGTTCAACGCCGTCGCGGCGGAGGATTTGATCGTAAAGGGCGGCAGAGTCGGCGGCGTCGTCACCAACTGGGCGCTGGTGTCGATGAACCATGACACACAGTCCTGCATGGACCCCAATGTGATGGAGGCCAAGGTGGTGGTCAGCTCTTGCGGCCACGATGGGCCCTTCGGGGCCACCGGAGTCAAGCGCCTCAGGAGCATCGGCATGATTGATTCCATCCCCGGAATGAAGGCCCTCGACATGAACACGGCGGAGGACGCCATTGTTAGGCTTACCCGAGAGGTCGTGCCCGGCATGATCGTTACCGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGGGCCGACATTCGGAGCGATGATGATATCGGGGCAGAAGGCGGCGCAGTTGGCGTTGAAGTCGTTGGGGCGAGAAAATTGCATTCGAGAAGAGGTTTTGGTTGAAGAAGCAGAAATGCAACTGGCGGCGGGGGAGTCGCCGGAGGTTGCAGATGCTTGAATTGGTTTGATTTGGAAGAAAAATTTGCTTTTTTTGGGTTTGTTTGTTGTTTGTACTGTGTAGTGAGTTTTCAGTTTTCAGTTTTCAGTTTTCAGTGATGAATGGCTCTGTTCGTAGCATAGCACGAGGGAAAGGGTTAGGGTTTGGGGAAAAGACAAAATATCACTTCCCTCGTTTCTTTTCTCTCTTTTATTTTTTTTAATAAAAATGCGTTCCAATTCCAAGGACAAGTTATTCGGTGAGGCCCGTGGTAAGGGTAACCACTAAATCAACTTTTAATTTGATTATTTCTATACAGATACATATTATATGCAGTGTATATAAAAATTTAAAGCACTCAGGCTCCCTCTAAGCCTTAACAATCTGTCTTTTTCAGTTCTCTTCCTAAATTACTGAGTGAGACCGTCTCATGCTAAAATAAAATTAATGAA

Coding sequence (CDS)

ATGGCCTCCATTGCCTCCACTCTTCCCACCAAGCTCCAAAGGCCCTCCTTACTCGATTCCTCTTTCCATGGCACCCCTTTGGCCCCTCCTCCTTCTCTCCGCCTCAAATCCTCCGCCGCCGCCTCTCTCTCCATCTCCGCCTCCGCCTCCGCCTCTCAACCGCCCTACGATTTGAACTCCTTCAAATTCAACCCCATCAAGGAGTCCATAGTCTCACGGGAGATGACCCGCCGCTACATGACCGACATGATCACTTACGCCGACACTGATGTTATCATCGTCGGTGCTGGCTCCGCCGGTCTCTCTTGCGCTTATGAGCTCAGCAAAGACCCCAATATCCGCATCGCCATCATCGAGCAGTCCGTCAGCCCCGGTGGCGGTGCATGGCTCGGTGGCCAGCTCTTCTCTGCTATGGTGGTACGGAAGCCAGCCCATAAATTCTTGGACGAGGTGGGTGTGGAGTACGACGAGCAAGATGACTACGTTGTAATCAAGCACGCGGCTCTGTTCACCTCAACGATCATGAGCAAGCTGCTGGCAAAGCCCAACGTCAAGCTGTTCAACGCCGTCGCGGCGGAGGATTTGATCGTAAAGGGCGGCAGAGTCGGCGGCGTCGTCACCAACTGGGCGCTGGTGTCGATGAACCATGACACACAGTCCTGCATGGACCCCAATGTGATGGAGGCCAAGGTGGTGGTCAGCTCTTGCGGCCACGATGGGCCCTTCGGGGCCACCGGAGTCAAGCGCCTCAGGAGCATCGGCATGATTGATTCCATCCCCGGAATGAAGGCCCTCGACATGAACACGGCGGAGGACGCCATTGTTAGGCTTACCCGAGAGGTCGTGCCCGGCATGATCGTTACCGGTATGGAGGTGGCGGAGATCGACGGAGCTCCAAGAATGGGGCCGACATTCGGAGCGATGATGATATCGGGGCAGAAGGCGGCGCAGTTGGCGTTGAAGTCGTTGGGGCGAGAAAATTGCATTCGAGAAGAGGTTTTGGTTGAAGAAGCAGAAATGCAACTGGCGGCGGGGGAGTCGCCGGAGGTTGCAGATGCTTGA

Protein sequence

MASIASTLPTKLQRPSLLDSSFHGTPLAPPPSLRLKSSAAASLSISASASASQPPYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLAKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDGPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPRMGPTFGAMMISGQKAAQLALKSLGRENCIREEVLVEEAEMQLAAGESPEVADA

Homology

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BLAST of Tan0020768 vs. ExPASy Swiss-Prot
Match: O23787 (Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=2 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 1.8e-153
Identity = 291/357 (81.51%), Postives = 313/357 (87.68%), Query Frame = 0


Query: 1   MASIASTLPTKLQRPSLLDSSFHGTPLAPPPSLRL---KSSAAASLSISASASASQPPYD 60
           MAS A           L DSSFHG P++ P  LRL   KSS   +LSISASAS   PPYD
Sbjct: 4   MASTAFAPSVSSTTNKLFDSSFHGAPMS-PSLLRLQPIKSSRPNNLSISASAS---PPYD 63

Query: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRIAI 120
           LN+FKF+PIKESIVSREMTRRYMTDMITYADTDV++VGAGSAGLSCAYELSK+PNI+IAI
Sbjct: 64  LNTFKFDPIKESIVSREMTRRYMTDMITYADTDVVVVGAGSAGLSCAYELSKNPNIQIAI 123

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G++YDEQD+YVVIKHAALFTSTIMSK
Sbjct: 124 IEQSVSPGGGAWLGGQLFSAMVVRKPAHIFLDELGIDYDEQDNYVVIKHAALFTSTIMSK 183

Query: 181 LLAKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLA+PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 184 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 243

Query: 241 HDGPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRL+SIGMI+ +PGMKALDMN+AEDAIVRLTREVVPGMIVTGMEVAEIDG
Sbjct: 244 HDGPFGATGVKRLKSIGMIEEVPGMKALDMNSAEDAIVRLTREVVPGMIVTGMEVAEIDG 303

Query: 301 APRMGPTFGAMMISGQKAAQLALKSLGRENCI-REEVLVEEAEMQLAAGESPEVADA 354
           APRMGPTFGAMMISGQKAA LALKSLG+ N +    V     E+ LAA +S E ADA
Sbjct: 304 APRMGPTFGAMMISGQKAAHLALKSLGQPNALDGTYVGGVHPELILAAADSAETADA 356

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BLAST of Tan0020768 vs. ExPASy Swiss-Prot
Match: Q38709 (Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=2 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 3.2e-147
Identity = 274/354 (77.40%), Postives = 309/354 (87.29%), Query Frame = 0


Query: 4   IASTLPTKLQRPSLLD---SSFHGTPLAPPPSLRLKSSAAASLSISASASASQPPYDLNS 63
           +ASTL +K QR +L +   SSF+GTPLA P S+R++ + A +    + + A  PPYDL +
Sbjct: 1   MASTLTSKPQRLALFENSASSFYGTPLA-PSSIRVQPTKAGAKPSISMSGAPSPPYDLKA 60

Query: 64  FKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRIAIIEQ 123
           F F+PIKESIVSREMTRRYM DMITYADTDV++VGAGS+GL C YELSK+P++++AIIEQ
Sbjct: 61  FTFDPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSSGLVC-YELSKNPSVQVAIIEQ 120

Query: 124 SVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 183
           SVSPGGGAWLGGQLFS MVVRKPAH FLDE+G+EYDEQD+YVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSGMVVRKPAHLFLDELGIEYDEQDNYVVIKHAALFTSTIMSKLLA 180

Query: 184 KPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 243
           +PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 244 PFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPR 303
           PFGATGVK LRSIGMID++PGMKALDMN AEDAIVRLTRE+VPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKSLRSIGMIDTVPGMKALDMNVAEDAIVRLTREIVPGMIVTGMEVAEIDGAPR 300

Query: 304 MGPTFGAMMISGQKAAQLALKSLGRENCIREEVLVE-EAEMQLAAGESPEVADA 354
           MGPTFGAMMISGQKAA LALK+LG  N +    +     E+ LAA +S E+ADA
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGLPNALDGSYVGGIHPELILAAADSAEIADA 352

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BLAST of Tan0020768 vs. ExPASy Swiss-Prot
Match: F6H7K5 (Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 2.7e-146
Identity = 279/360 (77.50%), Postives = 308/360 (85.56%), Query Frame = 0


Query: 1   MASIASTLP--TKLQRPSLLD--SSFHGTPLAPPPSLRLKSSAAASLSISASASASQPPY 60
           MAS+A+TL   +   +P+  D  SSFHG+P+    S R+     +  S + S S S  PY
Sbjct: 1   MASMATTLTSLSSNPKPAFFDNKSSFHGSPI----SYRVLPIKVSHQSPTISMS-SVSPY 60

Query: 61  DLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRIA 120
           DL SFKF PIKESIV+REMTRRYM DMITYADTDV+IVGAGSAGLSCAYELSK+P+IR+A
Sbjct: 61  DLQSFKFEPIKESIVAREMTRRYMMDMITYADTDVVIVGAGSAGLSCAYELSKNPSIRVA 120

Query: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIMS 180
           IIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDE+G+EYDEQD+YVVIKHAALFTSTIMS
Sbjct: 121 IIEQSVSPGGGAWLGGQLFSAMVVRKPAHHFLDELGIEYDEQDNYVVIKHAALFTSTIMS 180

Query: 181 KLLAKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240
           KLLA+PNVKLFNAVAAEDLIVK  RV GVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC
Sbjct: 181 KLLARPNVKLFNAVAAEDLIVKEERVAGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSC 240

Query: 241 GHDGPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEID 300
           GHDGPFGATGVKRL+SIGMIDS+PGMKALDMNTAEDAIVRLTRE+VPGMIVTGMEVAEID
Sbjct: 241 GHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREIVPGMIVTGMEVAEID 300

Query: 301 GAPRMGPTFGAMMISGQKAAQLALKSLGRENCI---REEVLVEEAEMQLAAGESPEVADA 354
           GAPRMGPTFGAMMISGQKAA LAL++LG+ N I     E    + E+ LAA E+ E+ DA
Sbjct: 301 GAPRMGPTFGAMMISGQKAAHLALRALGQPNAIDGNYTEAETMQPELILAAAETGEIVDA 355

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BLAST of Tan0020768 vs. ExPASy Swiss-Prot
Match: F6H9A9 (Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1 PE=3 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 7.9e-146
Identity = 270/358 (75.42%), Postives = 309/358 (86.31%), Query Frame = 0


Query: 1   MASIASTLPTKLQRPSLLD---SSFHGTPLAPPPSLRLKSSAAASLSISASASASQPPYD 60
           MA++ S++ +K  + S+ D   SSFHG P+A    L    S   +L+++A+A     PYD
Sbjct: 1   MATLTSSICSK-PKASVFDPHKSSFHGVPIATQARLSPVKSTPVNLAVTAAAM----PYD 60

Query: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRIAI 120
           L SFKF PIKESIVSREMTRRYM DMITYADTDV++VGAGSAGLSCAYELSK+P++++AI
Sbjct: 61  LRSFKFEPIKESIVSREMTRRYMMDMITYADTDVVVVGAGSAGLSCAYELSKNPSVQVAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFS+MVVRKPAH+FLDE+G+EYDEQD+YVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSSMVVRKPAHRFLDELGLEYDEQDNYVVIKHAALFTSTIMSK 180

Query: 181 LLAKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLA+PNVKLFNAVAAEDLI+K G+VGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIIKEGKVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRLRS+GMIDS+PGMKALDMNTAED IVRLTREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLRSVGMIDSVPGMKALDMNTAEDEIVRLTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAQLALKSLGRENCIREEVL--VEEAEMQLAAGESPEVADA 354
           +PRMGPTFGAMMISGQKAA LALKSLG  N +    +  +    +  AA ++ E+A+A
Sbjct: 301 SPRMGPTFGAMMISGQKAAHLALKSLGLPNALDGTYIGNLHPELVLAAAADAAEIAEA 353

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BLAST of Tan0020768 vs. ExPASy Swiss-Prot
Match: Q38814 (Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI1 PE=1 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 3.0e-145
Identity = 273/356 (76.69%), Postives = 302/356 (84.83%), Query Frame = 0


Query: 1   MASIASTLPTKLQRPS-LLDSSFHGTPL-APPPSLRLKSSAAASLSISASASASQPPYDL 60
           MA+IASTL     +P  L DSSFHG+ + A P S+ LK         S S  A+   YDL
Sbjct: 1   MAAIASTLSLSSTKPQRLFDSSFHGSAISAAPISIGLKPR-------SFSVRATTAGYDL 60

Query: 61  NSFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRIAII 120
           N+F F+PIKESIVSREMTRRYMTDMITYA+TDV++VGAGSAGLS AYE+SK+PN+++AII
Sbjct: 61  NAFTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAII 120

Query: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL 180
           EQSVSPGGGAWLGGQLFSAM+VRKPAH FLDE+GV YDEQD YVV+KHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKL 180

Query: 181 LAKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240
           LA+PNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGH 240

Query: 241 DGPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA 300
           DGPFGATGVKRL+SIGMID +PGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA 300

Query: 301 PRMGPTFGAMMISGQKAAQLALKSLGRENCIREEVLVE-EAEMQLAAGESPEVADA 354
           PRMGPTFGAMMISGQKA QLALK+LG  N I   ++     E+ LAA +S E  DA
Sbjct: 301 PRMGPTFGAMMISGQKAGQLALKALGLPNAIDGTLVGNLSPELVLAAADSAETVDA 349

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BLAST of Tan0020768 vs. NCBI nr
Match: XP_004152916.1 (thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149.1 hypothetical protein Csa_011133 [Cucumis sativus])

HSP 1 Score: 611.7 bits (1576), Expect = 4.0e-171
Identity = 324/361 (89.75%), Postives = 336/361 (93.07%), Query Frame = 0


Query: 1   MASIASTLPTKLQRPSLL-DSSFHGTPLAPPPSLRLKSSAAASLSISASASASQPPYDLN 60
           MASIASTL TKLQ+PSLL DSSFHGTPLA P SLRLKS+AA SL+I  SASASQPPYDLN
Sbjct: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTI--SASASQPPYDLN 60

Query: 61  SFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRIAIIE 120
            FKFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSK+PNIRIAIIE
Sbjct: 61  QFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIE 120

Query: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL 180
           QSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL
Sbjct: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL 180

Query: 181 AKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240
           A+PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD
Sbjct: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240

Query: 241 GPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAP 300
           GPFGATGVKRL+SIGMIDS+PGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAP
Sbjct: 241 GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAP 300

Query: 301 RMGPTFGAMMISGQKAAQLALKSLGRENCIREE-------VLVEEAEMQLAAGESPEVAD 354
           RMGPTFGAMMISGQKAA LALKSLG  N I +E       V +EE E+ LAAGESPEVAD
Sbjct: 301 RMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVAD 359

[Close]

BLAST of Tan0020768 vs. NCBI nr
Match: XP_038905461.1 (thiamine thiazole synthase, chloroplastic [Benincasa hispida])

HSP 1 Score: 610.1 bits (1572), Expect = 1.2e-170
Identity = 323/361 (89.47%), Postives = 336/361 (93.07%), Query Frame = 0


Query: 1   MASIASTLPTKLQRPS-LLDSSFHGTPLAPPPSLRLKS--SAAASLSISASASASQPPYD 60
           MASIASTL TKLQRPS LLDSSF+GTPLAPPPSLRLKS  +  A+ S+S SASASQPPYD
Sbjct: 1   MASIASTLTTKLQRPSLLLDSSFNGTPLAPPPSLRLKSTTTTTAATSLSISASASQPPYD 60

Query: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRIAI 120
           LN FKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSK+P+IRIAI
Sbjct: 61  LNQFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPSIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180

Query: 181 LLAKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLA+PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRL+SIG+IDS+PGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAQLALKSLGRENCIR-----EEVLVEEAEMQLAAGESPEVAD 354
           APRMGPTFGAMMISGQKAA LALKSLG  N I      E  +VEE E+ LAAGESPEVAD
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGEANGIEDGEVGERKVVEEPELLLAAGESPEVAD 360

[Close]

BLAST of Tan0020768 vs. NCBI nr
Match: XP_008463440.1 (PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo])

HSP 1 Score: 609.8 bits (1571), Expect = 1.5e-170
Identity = 324/361 (89.75%), Postives = 334/361 (92.52%), Query Frame = 0


Query: 1   MASIASTLPTKLQRPSLL-DSSFHGTPLAPPPSLRLKSSAAASLSISASASASQPPYDLN 60
           MASIASTL TKLQ PSLL DSSFHGTPLA PPSLRLKS AA SL+I  SASASQPPYDLN
Sbjct: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTI--SASASQPPYDLN 60

Query: 61  SFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRIAIIE 120
            FKFNPI+ESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSK+PNIRIAIIE
Sbjct: 61  QFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIE 120

Query: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL 180
           QSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL
Sbjct: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL 180

Query: 181 AKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240
           A+PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD
Sbjct: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240

Query: 241 GPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAP 300
           GPFGATGVKRL+SIG+IDS+PGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAP
Sbjct: 241 GPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAP 300

Query: 301 RMGPTFGAMMISGQKAAQLALKSLGRENCI-------REEVLVEEAEMQLAAGESPEVAD 354
           RMGPTFGAMMISGQKAA LALKSLG  N I         +V +EE E+ LAAGESPEVAD
Sbjct: 301 RMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVAD 359

[Close]

BLAST of Tan0020768 vs. NCBI nr
Match: KAG7031400.1 (Thiamine thiazole synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 603.2 bits (1554), Expect = 1.4e-168
Identity = 320/357 (89.64%), Postives = 332/357 (93.00%), Query Frame = 0


Query: 1   MASIASTLPTKLQRP-----SLLDSSFHGTPLAPPPSLRLKSSAAASLSISASASASQPP 60
           MAS+ASTL TKLQ P     SLLDSSFHGTPLAPPPSLRLK +  AS SISASAS  Q P
Sbjct: 1   MASMASTLTTKLQTPSSSSSSLLDSSFHGTPLAPPPSLRLKPT-PASFSISASAS-QQQP 60

Query: 61  YDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRI 120
           YDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSK+P+IRI
Sbjct: 61  YDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRI 120

Query: 121 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIM 180
           AIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIM
Sbjct: 121 AIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIM 180

Query: 181 SKLLAKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240
           SKLLA+PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS
Sbjct: 181 SKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSS 240

Query: 241 CGHDGPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEI 300
           CGHDGPFGATGVKRL+SIGMIDS+PGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEI
Sbjct: 241 CGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEI 300

Query: 301 DGAPRMGPTFGAMMISGQKAAQLALKSLGRENCIREEVLVEEAEMQLAAGESPEVAD 353
           DGAPRMGPTFGAMMISGQKAA LALKSLG  NCI +E     A++ LAAGESPEVAD
Sbjct: 301 DGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA----ADLILAAGESPEVAD 351

[Close]

BLAST of Tan0020768 vs. NCBI nr
Match: KAG6600763.1 (Thiamine thiazole synthase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 600.5 bits (1547), Expect = 9.3e-168
Identity = 319/358 (89.11%), Postives = 331/358 (92.46%), Query Frame = 0


Query: 1   MASIASTLPTKLQRP------SLLDSSFHGTPLAPPPSLRLKSSAAASLSISASASASQP 60
           MAS+ASTL  KLQ P      SLLDSSFHGTPLAPPPSLRLK +  AS SISASAS  Q 
Sbjct: 1   MASMASTLTIKLQTPSSSSSSSLLDSSFHGTPLAPPPSLRLKPT-PASFSISASAS-QQQ 60

Query: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIR 120
           PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSK+P+IR
Sbjct: 61  PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIR 120

Query: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTI 180
           IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTI
Sbjct: 121 IAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTI 180

Query: 181 MSKLLAKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240
           MSKLLA+PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS
Sbjct: 181 MSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVS 240

Query: 241 SCGHDGPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300
           SCGHDGPFGATGVKRL+SIGMIDS+PGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE
Sbjct: 241 SCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAE 300

Query: 301 IDGAPRMGPTFGAMMISGQKAAQLALKSLGRENCIREEVLVEEAEMQLAAGESPEVAD 353
           IDGAPRMGPTFGAMMISGQKAA LALKSLG  NCI +E     A++ LAAGESPEVAD
Sbjct: 301 IDGAPRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA----ADLILAAGESPEVAD 352

[Close]

BLAST of Tan0020768 vs. ExPASy TrEMBL
Match: A0A0A0L546 (Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=3 SV=1)

HSP 1 Score: 611.7 bits (1576), Expect = 1.9e-171
Identity = 324/361 (89.75%), Postives = 336/361 (93.07%), Query Frame = 0


Query: 1   MASIASTLPTKLQRPSLL-DSSFHGTPLAPPPSLRLKSSAAASLSISASASASQPPYDLN 60
           MASIASTL TKLQ+PSLL DSSFHGTPLA P SLRLKS+AA SL+I  SASASQPPYDLN
Sbjct: 1   MASIASTLTTKLQKPSLLHDSSFHGTPLASPSSLRLKSTAATSLTI--SASASQPPYDLN 60

Query: 61  SFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRIAIIE 120
            FKFNPI+ESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSK+PNIRIAIIE
Sbjct: 61  QFKFNPIRESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPNIRIAIIE 120

Query: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL 180
           QSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL
Sbjct: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL 180

Query: 181 AKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240
           A+PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD
Sbjct: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240

Query: 241 GPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAP 300
           GPFGATGVKRL+SIGMIDS+PGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAP
Sbjct: 241 GPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAP 300

Query: 301 RMGPTFGAMMISGQKAAQLALKSLGRENCIREE-------VLVEEAEMQLAAGESPEVAD 354
           RMGPTFGAMMISGQKAA LALKSLG  N I +E       V +EE E+ LAAGESPEVAD
Sbjct: 301 RMGPTFGAMMISGQKAAHLALKSLGEANSIGDEGEMGERKVGLEEEELLLAAGESPEVAD 359

[Close]

BLAST of Tan0020768 vs. ExPASy TrEMBL
Match: A0A1S3CJ81 (Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501605 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 7.4e-171
Identity = 324/361 (89.75%), Postives = 334/361 (92.52%), Query Frame = 0


Query: 1   MASIASTLPTKLQRPSLL-DSSFHGTPLAPPPSLRLKSSAAASLSISASASASQPPYDLN 60
           MASIASTL TKLQ PSLL DSSFHGTPLA PPSLRLKS AA SL+I  SASASQPPYDLN
Sbjct: 1   MASIASTLTTKLQIPSLLHDSSFHGTPLASPPSLRLKSPAATSLTI--SASASQPPYDLN 60

Query: 61  SFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRIAIIE 120
            FKFNPI+ESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSK+PNIRIAIIE
Sbjct: 61  QFKFNPIRESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKNPNIRIAIIE 120

Query: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL 180
           QSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL
Sbjct: 121 QSVSPGGGAWLGGQLFSAMVVRKPAHLFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLL 180

Query: 181 AKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240
           A+PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD
Sbjct: 181 ARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHD 240

Query: 241 GPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAP 300
           GPFGATGVKRL+SIG+IDS+PGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAP
Sbjct: 241 GPFGATGVKRLKSIGLIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAP 300

Query: 301 RMGPTFGAMMISGQKAAQLALKSLGRENCI-------REEVLVEEAEMQLAAGESPEVAD 354
           RMGPTFGAMMISGQKAA LALKSLG  N I         +V +EE E+ LAAGESPEVAD
Sbjct: 301 RMGPTFGAMMISGQKAAHLALKSLGEANSIGGEGEMGERKVGLEEEELLLAAGESPEVAD 359

[Close]

BLAST of Tan0020768 vs. ExPASy TrEMBL
Match: A0A6J1JNT3 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487533 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 7.7e-168
Identity = 321/360 (89.17%), Postives = 333/360 (92.50%), Query Frame = 0


Query: 1   MASIASTLPTKLQRP-----SLLDSSFHGTPLA---PPPSLRLKSSAAASLSISASASAS 60
           MAS+AST  TKLQ P     SLLDSSFHGTPLA   PPPSLRLKS+ AA  SISASAS  
Sbjct: 1   MASMASTFTTKLQTPSSSSSSLLDSSFHGTPLAPPPPPPSLRLKSTPAA-FSISASASQH 60

Query: 61  QPPYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPN 120
           Q PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSK+P+
Sbjct: 61  Q-PYDLNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPD 120

Query: 121 IRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTS 180
           IRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTS
Sbjct: 121 IRIAIIEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTS 180

Query: 181 TIMSKLLAKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVV 240
           TIMSKLLA+PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVV
Sbjct: 181 TIMSKLLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVV 240

Query: 241 VSSCGHDGPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEV 300
           VSSCGHDGPFGATGVKRL+SIGMIDS+PGMKALDMNTAEDAIVRLTREVVPGMIVTGMEV
Sbjct: 241 VSSCGHDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEV 300

Query: 301 AEIDGAPRMGPTFGAMMISGQKAAQLALKSLGRENCIREEVLVEEAEMQLAAGESPEVAD 353
           AEIDGAPRMGPTFGAMMISGQKAA LALKSLG ENCI +E     A++ LAAGESPEVAD
Sbjct: 301 AEIDGAPRMGPTFGAMMISGQKAAHLALKSLGLENCIEDEA----ADLILAAGESPEVAD 354

[Close]

BLAST of Tan0020768 vs. ExPASy TrEMBL
Match: A0A6J1J7L4 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483293 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 7.7e-168
Identity = 312/358 (87.15%), Postives = 332/358 (92.74%), Query Frame = 0


Query: 1   MASIASTLPTKLQRPSLLDSSFHGTPLAPPPSLRLKSSAAASLSISASASASQPPYDLNS 60
           MASIAS+LPTKL RPSLLDSSFHG+PL P PS RLK + AA+ S+S SASA+QPPYDLN 
Sbjct: 1   MASIASSLPTKLHRPSLLDSSFHGSPLLPTPSFRLKPTTAAA-SLSISASAAQPPYDLNQ 60

Query: 61  FKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRIAIIEQ 120
           FKFNPI+ESIVSREMTRRYMTDMITYADTDVI+VGAGSAGLSCAYELSKDP++R+AIIEQ
Sbjct: 61  FKFNPIRESIVSREMTRRYMTDMITYADTDVIVVGAGSAGLSCAYELSKDPSVRVAIIEQ 120

Query: 121 SVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKLLA 180
           SVSPGGGAWLGGQLFSAMVVRKPAH FLDE+ VEYDEQ+DYVVIKHAALFTSTIMSKLLA
Sbjct: 121 SVSPGGGAWLGGQLFSAMVVRKPAHYFLDELEVEYDEQEDYVVIKHAALFTSTIMSKLLA 180

Query: 181 KPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240
           +PNVKLFNAVAAEDLIVK GRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG
Sbjct: 181 RPNVKLFNAVAAEDLIVKEGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGHDG 240

Query: 241 PFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGAPR 300
           PFGATGVKRL+SIGMIDS+PGMKALDMNTAEDAIVR TREVVPGMIVTGMEVAEIDGAPR
Sbjct: 241 PFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRFTREVVPGMIVTGMEVAEIDGAPR 300

Query: 301 MGPTFGAMMISGQKAAQLALKSLGRENCIREE-----VLVEEAEMQLAAGESPEVADA 354
           MGPTFGAMMISGQKAA LALK+LG+ N I E+       VE AEM LAAGE+PEVADA
Sbjct: 301 MGPTFGAMMISGQKAAHLALKALGKANSIDEKGENGGRKVEAAEMVLAAGETPEVADA 357

[Close]

BLAST of Tan0020768 vs. ExPASy TrEMBL
Match: A0A6J1FWL6 (Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448035 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.3e-167
Identity = 319/355 (89.86%), Postives = 331/355 (93.24%), Query Frame = 0


Query: 1   MASIASTLPTKLQRP--SLLDSSFHGTPLA-PPPSLRLKSSAAASLSISASASASQPPYD 60
           MAS+ASTL TKLQ P  SLLDSSFHGTPLA PPPSLRLKS+ AA  SISAS    Q PYD
Sbjct: 1   MASVASTLTTKLQTPSSSLLDSSFHGTPLAPPPPSLRLKSTPAA-FSISAS---QQQPYD 60

Query: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRIAI 120
           LNSFKFNPIKESIVSREMTRRYMTDMITYADTDV+IVGAGSAGLSCAYELSK+P+IRIAI
Sbjct: 61  LNSFKFNPIKESIVSREMTRRYMTDMITYADTDVVIVGAGSAGLSCAYELSKNPDIRIAI 120

Query: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIMSK 180
           IEQSVSPGGGAWLGGQLFSAMVVRKPAH FLDEVGVEYDEQ+DYVVIKHAALFTSTIMSK
Sbjct: 121 IEQSVSPGGGAWLGGQLFSAMVVRKPAHCFLDEVGVEYDEQEDYVVIKHAALFTSTIMSK 180

Query: 181 LLAKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240
           LLA+PNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG
Sbjct: 181 LLARPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCG 240

Query: 241 HDGPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300
           HDGPFGATGVKRL+SIGMIDS+PGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG
Sbjct: 241 HDGPFGATGVKRLKSIGMIDSVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDG 300

Query: 301 APRMGPTFGAMMISGQKAAQLALKSLGRENCIREEVLVEEAEMQLAAGESPEVAD 353
           APRMGPTFGAMMISGQKAA LALKSLG  NCI +E     A++ LAAGESPEVAD
Sbjct: 301 APRMGPTFGAMMISGQKAAHLALKSLGLANCIEDEA----ADLILAAGESPEVAD 347

[Close]

BLAST of Tan0020768 vs. TAIR 10
Match: AT5G54770.1 (thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4) )

HSP 1 Score: 516.2 bits (1328), Expect = 2.1e-146
Identity = 273/356 (76.69%), Postives = 302/356 (84.83%), Query Frame = 0


Query: 1   MASIASTLPTKLQRPS-LLDSSFHGTPL-APPPSLRLKSSAAASLSISASASASQPPYDL 60
           MA+IASTL     +P  L DSSFHG+ + A P S+ LK         S S  A+   YDL
Sbjct: 1   MAAIASTLSLSSTKPQRLFDSSFHGSAISAAPISIGLKPR-------SFSVRATTAGYDL 60

Query: 61  NSFKFNPIKESIVSREMTRRYMTDMITYADTDVIIVGAGSAGLSCAYELSKDPNIRIAII 120
           N+F F+PIKESIVSREMTRRYMTDMITYA+TDV++VGAGSAGLS AYE+SK+PN+++AII
Sbjct: 61  NAFTFDPIKESIVSREMTRRYMTDMITYAETDVVVVGAGSAGLSAAYEISKNPNVQVAII 120

Query: 121 EQSVSPGGGAWLGGQLFSAMVVRKPAHKFLDEVGVEYDEQDDYVVIKHAALFTSTIMSKL 180
           EQSVSPGGGAWLGGQLFSAM+VRKPAH FLDE+GV YDEQD YVV+KHAALFTSTIMSKL
Sbjct: 121 EQSVSPGGGAWLGGQLFSAMIVRKPAHLFLDEIGVAYDEQDTYVVVKHAALFTSTIMSKL 180

Query: 181 LAKPNVKLFNAVAAEDLIVKGGRVGGVVTNWALVSMNHDTQSCMDPNVMEAKVVVSSCGH 240
           LA+PNVKLFNAVAAEDLIVKG RVGGVVTNWALV+ NH TQSCMDPNVMEAK+VVSSCGH
Sbjct: 181 LARPNVKLFNAVAAEDLIVKGNRVGGVVTNWALVAQNHHTQSCMDPNVMEAKIVVSSCGH 240

Query: 241 DGPFGATGVKRLRSIGMIDSIPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA 300
           DGPFGATGVKRL+SIGMID +PGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA
Sbjct: 241 DGPFGATGVKRLKSIGMIDHVPGMKALDMNTAEDAIVRLTREVVPGMIVTGMEVAEIDGA 300

Query: 301 PRMGPTFGAMMISGQKAAQLALKSLGRENCIREEVLVE-EAEMQLAAGESPEVADA 354
           PRMGPTFGAMMISGQKA QLALK+LG  N I   ++     E+ LAA +S E  DA
Sbjct: 301 PRMGPTFGAMMISGQKAGQLALKALGLPNAIDGTLVGNLSPELVLAAADSAETVDA 349

The following BLAST results are available for this feature:

BLAST of Tan0020768 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O23787	1.8e-153	81.51	Thiamine thiazole synthase, chloroplastic OS=Citrus sinensis OX=2711 GN=THI1 PE=...	[more]
Q38709	3.2e-147	77.40	Thiamine thiazole synthase, chloroplastic OS=Alnus glutinosa OX=3517 GN=THI1 PE=...	[more]
F6H7K5	2.7e-146	77.50	Thiamine thiazole synthase 2, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-2...	[more]
F6H9A9	7.9e-146	75.42	Thiamine thiazole synthase 1, chloroplastic OS=Vitis vinifera OX=29760 GN=THI1-1...	[more]
Q38814	3.0e-145	76.69	Thiamine thiazole synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THI...	[more]

BLAST of Tan0020768 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004152916.1	4.0e-171	89.75	thiamine thiazole synthase, chloroplastic [Cucumis sativus] >KGN56149.1 hypothet...	[more]
XP_038905461.1	1.2e-170	89.47	thiamine thiazole synthase, chloroplastic [Benincasa hispida]	[more]
XP_008463440.1	1.5e-170	89.75	PREDICTED: thiamine thiazole synthase, chloroplastic [Cucumis melo]	[more]
KAG7031400.1	1.4e-168	89.64	Thiamine thiazole synthase 2, chloroplastic [Cucurbita argyrosperma subsp. argyr...	[more]
KAG6600763.1	9.3e-168	89.11	Thiamine thiazole synthase 2, chloroplastic, partial [Cucurbita argyrosperma sub...	[more]

BLAST of Tan0020768 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L546	1.9e-171	89.75	Thiamine thiazole synthase, chloroplastic OS=Cucumis sativus OX=3659 GN=THI1 PE=...	[more]
A0A1S3CJ81	7.4e-171	89.75	Thiamine thiazole synthase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC10350160...	[more]
A0A6J1JNT3	7.7e-168	89.17	Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1J7L4	7.7e-168	87.15	Thiamine thiazole synthase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1FWL6	1.3e-167	89.86	Thiamine thiazole synthase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]

BLAST of Tan0020768 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54770.1	2.1e-146	76.69	thiazole biosynthetic enzyme, chloroplast (ARA6) (THI1) (THI4)	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF01946	Thi4	coord: 72..305 e-value: 2.5E-114 score: 380.4
None	No IPR available	GENE3D	6.10.250.2840		coord: 57..87 e-value: 2.4E-19 score: 70.6
None	No IPR available	PANTHER	PTHR43422	THIAMINE THIAZOLE SYNTHASE	coord: 1..353
None	No IPR available	PANTHER	PTHR43422:SF11	THIAMINE THIAZOLE SYNTHASE, CHLOROPLASTIC	coord: 1..353
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 88..323 e-value: 1.1E-121 score: 406.6
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 49..319
IPR002922	Thiazole biosynthetic enzyme Thi4 family	TIGRFAM	TIGR00292	TIGR00292	coord: 68..323 e-value: 1.6E-82 score: 274.6
IPR027495	Thiamine thiazole synthase	HAMAP	MF_03158	THI4	coord: 5..332 score: 68.120224

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0020768.1	Tan0020768.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009228	thiamine biosynthetic process
biological_process	GO:0052837	thiazole biosynthetic process
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005829	cytosol
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016763	pentosyltransferase activity